SMED30000998

Overview
NameSMED30000998
Smed IDSMED30000998
Length (bp)4090
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30000998 (SMED30000998) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30000998 (SMED30000998) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30000998 (SMED30000998) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30000998 (SMED30000998) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30000998

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30000998SMESG000008901.1 SmedASXL_014575SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
gutSMED30000998SMESG000008901.1 dd_Smed_v4_15396_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30000998SMESG000008901.1 dd_Smed_v4_15396_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30000998SMESG000008901.1 dd_Smed_v6_16492_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
reproductive organSMED30000998SMESG000008901.1 Contig29814newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30000998SMESG000008901.1 Contig29814uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30000998SMESG000008901.1 Contig23664newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30000998SMESG000008901.1 Contig23664uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30000998 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001534:77043..104062 -20147
Homology
[Close]
BLAST of SMED30000998 vs. Planmine SMEST
Match: SMESG000008901.1 (SMESG000008901.1)

HSP 1 Score: 1862.81 bits (4824), Expect = 0.000e+0
Identity = 944/956 (98.74%), Postives = 944/956 (98.74%), Query Frame = 3 
Query:   75 MIDQGTNDSYNDCHNVLDFDDSDVLMSNNFDTHSCKENNNIKNGEMQSNIYGQIERLSNEKVLIQNQCNSKMIDLQAQIMKLTNNFERIETSKTELEVELTKCHKDLRQTQRNFQIKSNELENLKIEFSNKLNSLESDYMNKISHANNLNKSLQEQICEEKRNNKNNQDFLSDKINQMFSKIKEKDEELSNNLTIQNHLQKTLTELEQQISHLTKKIEESDHMNAFHQQELHEKKEQIESLRNSELKLKSLVTELTRKVTVVESKMFEEKHELNESKSYXXXXXXXXXXXXXXXVIKEKEYNILLDRITISNKKIKELEEALSNQMQISSNYHEHLKKFENKQKIQSIDIKDQNENLARKVAQLEETIKIQSRIIEETNEELSTSRECEGTAVSNLNLTLEELDKLIEEPKMYLKSPGKSRVIGSPKIVTEFLNDSFLSPSNSCLLAKSGDHCLSPTPNKRIIRNVKQIIRDFQEHIRSLDNDKLEGIDKIQTQDKEIKNLKIQVRKYTTQNKALEIDNTEKQSEIENLTEEISKLKSLIASLKIENAKNKTNYKENVENLKSSIVRVSEESQNNIESRDKYLQNISMIIKEALQNNFTSYLDWPKFYGCRNSNFTKSNLESAFNESATQTHFADNIAKENLELTGNMTGTFCFSNQLNPPPTTTFETNLKPVEKRSRIHENNHDKKAENRIITVNDERKKSAKKVPRLPDGKKDPYVDSAQSWPALREVISNELSALLGRCDIQRAQ------------CHNLMSELSTKENIIARLLAYQNSSSFNNQGPVDTTSQCPNENAVEIPTNNNKTLIVKPNFSNQNNQTRSNIDLKKKTKVSLKSRVDNMSSNTSNKRCNKVENQLSLTNAPCCCGDNETRYQFTNEPDRKICTYKQESNNNIHQNPRKINWKSIEQQPNNDPVTNEIISEQLVCDETDYNLTSEYAASDFEVSKDC 2906
            MIDQGTNDSYNDCHNVLDFDDSDVLMSNNFDTHSCKENNNIKNGEMQSNIYGQIERLSNEKVLIQNQCNSKMIDLQAQIMKLTNNFERIETSKTELEVELTKCHKDLRQTQRNFQIKSNELENLKIEFSNKLNSLESDYMNKISHANNLNKSLQEQICEEKRNNKNNQDFLSDKINQMFSKIKEKDEELSNNLTIQNHLQKTLTELEQQISHLTKKIEESDHMNAFHQQELHEKKEQIESLRNSELKLKSLVTELTRKVTVVESKMFEEKHELNESKSYQNNLQAQLLDLQQAQVIKEKEYNILLDRITISNKKIKELEEALSNQMQISSNYHEHLKKFENKQKIQSIDIKDQNENLARKVAQLEETIKIQSRIIEETNEELSTSRECEGTAVSNLNLTLEELDKLIEEPKMYLKSPGKSRVIGSPKIVTEFLNDSFLSPSNSCLLAKSGDHCLSPTPNKRIIRNVKQIIRDFQEHIRSLDNDKLEGIDKIQTQDKEIKNLKIQVRKYTTQNKALEIDNTEKQSEIENLTEEISKLKSLIASLKIENAKNKTNYKENVENLKSSIVRVSEESQNNIESRDKYLQNISMIIKEALQNNFTSYLDWPKFYGCRNSNFTKSNLESAFNESATQTHFADNIAKENLELTGNMTGTFCFSNQLNPPPTTTFETNLKPVEKRSRIHENNHDKKAENRIITVNDERKKSAKKVPRLPDGKKDPYVDSAQSWPALREVISNELSALLGRCDIQRAQ            CHNLMSELSTKENIIARLLAYQNSSSFNNQGPVDTTSQCPNENAVEIPTNNNKTLIVKPNFSNQNNQTRSNIDLKKKTKVSLKSRVDNMSSNTSNKRCNKVENQLSLTNAPCCCGDNETRYQFTNEPDRKICTYKQESNNNIHQNPRKINWKSIEQQPNNDPVTNEIISEQLVCDETDYNLTSEYAASDFEVSKDC
Sbjct:    1 MIDQGTNDSYNDCHNVLDFDDSDVLMSNNFDTHSCKENNNIKNGEMQSNIYGQIERLSNEKVLIQNQCNSKMIDLQAQIMKLTNNFERIETSKTELEVELTKCHKDLRQTQRNFQIKSNELENLKIEFSNKLNSLESDYMNKISHANNLNKSLQEQICEEKRNNKNNQDFLSDKINQMFSKIKEKDEELSNNLTIQNHLQKTLTELEQQISHLTKKIEESDHMNAFHQQELHEKKEQIESLRNSELKLKSLVTELTRKVTVVESKMFEEKHELNESKSYQNNLQAQLLDLQQAQVIKEKEYNILLDRITISNKKIKELEEALSNQMQISSNYHEHLKKFENKQKIQSIDIKDQNENLARKVAQLEETIKIQSRIIEETNEELSTSRECEGTAVSNLNLTLEELDKLIEEPKMYLKSPGKSRVIGSPKIVTEFLNDSFLSPSNSCLLAKSGDHCLSPTPNKRIIRNVKQIIRDFQEHIRSLDNDKLEGIDKIQTQDKEIKNLKIQVRKYTTQNKALEIDNTEKQSEIENLTEEISKLKSLIASLKIENAKNKTNYKENVENLKSSIVRVSEESQNNIESRDKYLQNISMIIKEALQNNFTSYLDWPKFYGCRNSNFTKSNLESAFNESATQTHFADNIAKENLELTGNMTGTFCFSNQLNPPPTTTFETNLKPVEKRSRIHENNHDKKAENRIITVNDERKKSAKKVPRLPDGKKDPYVDSAQSWPALREVISNELSALLGRCDIQRAQCRIVPNEAINVNCHNLMSELSTKENIIARLLAYQNSSSFNNQGPVDTTSQCPNENAVEIPTNNNKTLIVKPNFSNQNNQTRSNIDLKKKTKVSLKSRVDNMSSNTSNKRCNKVENQLSLTNAPCCCGDNETRYQFTNEPDRKICTYKQESNNNIHQNPRKINWKSIEQQPNNDPVTNEIISEQLVCDETDYNLTSEYAASDFEVSKDC 956
[Close]
BLAST of SMED30000998 vs. Planmine SMEST
Match: SMESG000008901.1 (SMESG000008901.1)

HSP 1 Score: 1659.81 bits (4297), Expect = 0.000e+0
Identity = 854/870 (98.16%), Postives = 855/870 (98.28%), Query Frame = 3 
Query:   75 MIDQGTNDSYNDCHNVLDFDDSDVLMSNNFDTHSCKENNNIKNGEMQSNIYGQIERLSNEKVLIQNQCNSKMIDLQAQIMKLTNNFERIETSKTELEVELTKCHKDLRQTQRNFQIKSNELENLKIEFSNKLNSLESDYMNKISHANNLNKSLQEQICEEKRNNKNNQDFLSDKINQMFSKIKEKDEELSNNLTIQNHLQKTLTELEQQISHLTKKIEESDHMNAFHQQELHEKKEQIESLRNSELKLKSLVTELTRKVTVVESKMFEEKHELNESKSYXXXXXXXXXXXXXXXVIKEKEYNILLDRITISNKKIKELEEALSNQMQISSNYHEHLKKFENKQKIQSIDIKDQNENLARKVAQLEETIKIQSRIIEETNEELSTSRECEGTAVSNLNLTLEELDKLIEEPKMYLKSPGKSRVIGSPKIVTEFLNDSFLSPSNSCLLAKSGDHCLSPTPNKRIIRNVKQIIRDFQEHIRSLDNDKLEGIDKIQTQDKEIKNLKIQVRKYTTQNKALEIDNTEKQSEIENLTEEISKLKSLIASLKIENAKNKTNYKENVENLKSSIVRVSEESQNNIESRDKYLQNISMIIKEALQNNFTSYLDWPKFYGCRNSNFTKSNLESAFNESATQTHFADNIAKENLELTGNMTGTFCFSNQLNPPPTTTFETNLKPVEKRSRIHENNHDKKAENRIITVNDERKKSAKKVPRLPDGKKDPYVDSAQSWPALREVISNELSALLGRCDIQRAQ------------CHNLMSELSTKENIIARLLAYQNSSSFNNQGPVDTTSQCPNENAVEIPTNNNKTLIVKPNFSNQNNQTRSNIDLKKKTKVSLKSRVDNMSSNTSNKRCNKVENQLSLTNA 2648
            MIDQGTNDSYNDCHNVLDFDDSDVLMSNNFDTHSCKENNNIKNGEMQSNIYGQIERLSNEKVLIQNQCNSKMIDLQAQIMKLTNNFERIETSKTELEVELTKCHKDLRQTQRNFQIKSNELENLKIEFSNKLNSLESDYMNKISHANNLNKSLQEQICEEKRNNKNNQDFLSDKINQMFSKIKEKDEELSNNLTIQNHLQKTLTELEQQISHLTKKIEESDHMNAFHQQELHEKKEQIESLRNSELKLKSLVTELTRKVTVVESKMFEEKHELNESKSYQNNLQAQLLDLQQAQVIKEKEYNILLDRITISNKKIKELEEALSNQMQISSNYHEHLKKFENKQKIQSIDIKDQNENLARKVAQLEETIKIQSRIIEETNEELSTSRECEGTAVSNLNLTLEELDKLIEEPKMYLKSPGKSRVIGSPKIVTEFLNDSFLSPSNSCLLAKSGDHCLSPTPNKRIIRNVKQIIRDFQEHIRSLDNDKLEGIDKIQTQDKEIKNLKIQVRKYTTQNKALEIDNTEKQSEIENLTEEISKLKSLIASLKIENAKNKTNYKENVENLKSSIVRVSEESQNNIESRDKYLQNISMIIKEALQNNFTSYLDWPKFYGCRNSNFTKSNLESAFNESATQTHFADNIAKENLELTGNMTGTFCFSNQLNPPPTTTFETNLKPVEKRSRIHENNHDKKAENRIITVNDERKKSAKKVPRLPDGKKDPYVDSAQSWPALREVISNELSALLGRCDIQRAQ            CHNLMSELSTKENIIARLLAYQNSSSFNNQGPVDTTSQCPNENAVEIPTNNNKTLIVKPNFSNQNNQTRSNIDLKKKTKVSLKSRVDNMSSNTSNKRCNKVENQ   TN+
Sbjct:    1 MIDQGTNDSYNDCHNVLDFDDSDVLMSNNFDTHSCKENNNIKNGEMQSNIYGQIERLSNEKVLIQNQCNSKMIDLQAQIMKLTNNFERIETSKTELEVELTKCHKDLRQTQRNFQIKSNELENLKIEFSNKLNSLESDYMNKISHANNLNKSLQEQICEEKRNNKNNQDFLSDKINQMFSKIKEKDEELSNNLTIQNHLQKTLTELEQQISHLTKKIEESDHMNAFHQQELHEKKEQIESLRNSELKLKSLVTELTRKVTVVESKMFEEKHELNESKSYQNNLQAQLLDLQQAQVIKEKEYNILLDRITISNKKIKELEEALSNQMQISSNYHEHLKKFENKQKIQSIDIKDQNENLARKVAQLEETIKIQSRIIEETNEELSTSRECEGTAVSNLNLTLEELDKLIEEPKMYLKSPGKSRVIGSPKIVTEFLNDSFLSPSNSCLLAKSGDHCLSPTPNKRIIRNVKQIIRDFQEHIRSLDNDKLEGIDKIQTQDKEIKNLKIQVRKYTTQNKALEIDNTEKQSEIENLTEEISKLKSLIASLKIENAKNKTNYKENVENLKSSIVRVSEESQNNIESRDKYLQNISMIIKEALQNNFTSYLDWPKFYGCRNSNFTKSNLESAFNESATQTHFADNIAKENLELTGNMTGTFCFSNQLNPPPTTTFETNLKPVEKRSRIHENNHDKKAENRIITVNDERKKSAKKVPRLPDGKKDPYVDSAQSWPALREVISNELSALLGRCDIQRAQCRIVPNEAINVNCHNLMSELSTKENIIARLLAYQNSSSFNNQGPVDTTSQCPNENAVEIPTNNNKTLIVKPNFSNQNNQTRSNIDLKKKTKVSLKSRVDNMSSNTSNKRCNKVENQKPDTNS 870

HSP 2 Score: 455.292 bits (1170), Expect = 1.578e-137
Identity = 226/226 (100.00%), Postives = 226/226 (100.00%), Query Frame = 2 
Query: 3368 FDCDLVSKDFLDSILNDFVNFCFDHNNRYLLVSKPSKCVTSNIRVSSNTIVLDKLKSNVHTIQTIIETQISKKFYMSKKKLYQNDVQTLIVKIKEMGQIIDVQNLVIEQRQNSIVQLEEKLIQQNDENLHLISSMRQVESLNQLQILEIESLKLLIDKTKAYLIALVEMCNQYSNCEIFNCAQLMEEEASIFHSIISDDCNDFINFVRNGIRAALRLSCSVKSIFI 4045
            FDCDLVSKDFLDSILNDFVNFCFDHNNRYLLVSKPSKCVTSNIRVSSNTIVLDKLKSNVHTIQTIIETQISKKFYMSKKKLYQNDVQTLIVKIKEMGQIIDVQNLVIEQRQNSIVQLEEKLIQQNDENLHLISSMRQVESLNQLQILEIESLKLLIDKTKAYLIALVEMCNQYSNCEIFNCAQLMEEEASIFHSIISDDCNDFINFVRNGIRAALRLSCSVKSIFI
Sbjct: 1081 FDCDLVSKDFLDSILNDFVNFCFDHNNRYLLVSKPSKCVTSNIRVSSNTIVLDKLKSNVHTIQTIIETQISKKFYMSKKKLYQNDVQTLIVKIKEMGQIIDVQNLVIEQRQNSIVQLEEKLIQQNDENLHLISSMRQVESLNQLQILEIESLKLLIDKTKAYLIALVEMCNQYSNCEIFNCAQLMEEEASIFHSIISDDCNDFINFVRNGIRAALRLSCSVKSIFI 1306

HSP 3 Score: 452.595 bits (1163), Expect = 1.691e-136
Identity = 219/231 (94.81%), Postives = 221/231 (95.67%), Query Frame = 1 
Query: 2659 VAIMKPDTNSQMNRIGKFAHTNKRATTISTKIPERSIGSPSNNNPTMIQLPTKSSVNNLCVTKPIIISLPSTPPVTSRCRKTVNDESPQFNELIVDQRLTALILGTLINVIHLRSELETNKSLIKFYKLLYLRSVDCSQLISVNEHLECMISRSHLHSRHVNVQRKPKRFRIYAHVVMACCYISKRLLASKLIICTDPEVMISDDLKGFVHNVINSVPDSVIILELGFDFI 3351
            V   KPDTNSQMNRIGKFAHTNKRATTISTKIPERSIGSPSNNNPTMIQLPTKSSVNNLCVTKPIIISLPSTPPVTSRCRKTVNDESPQFNELIVDQRLTALILGTLINVIHLRSELETNKSLIKFYKLLYLRSVDCSQLISVNEHLECMISRSHLHSRHVNVQRKPKRFRIYAHVVMACCYISKRLLASKLIICTDPEVMISDDLKGFVHNVINSVPDS     +  DF+
Sbjct:  861 VENQKPDTNSQMNRIGKFAHTNKRATTISTKIPERSIGSPSNNNPTMIQLPTKSSVNNLCVTKPIIISLPSTPPVTSRCRKTVNDESPQFNELIVDQRLTALILGTLINVIHLRSELETNKSLIKFYKLLYLRSVDCSQLISVNEHLECMISRSHLHSRHVNVQRKPKRFRIYAHVVMACCYISKRLLASKLIICTDPEVMISDDLKGFVHNVINSVPDSFDCDLVSKDFL 1091
[Close]
BLAST of SMED30000998 vs. Planmine SMEST
Match: SMESG000008901.1 (SMESG000008901.1)

HSP 1 Score: 1623.99 bits (4204), Expect = 0.000e+0
Identity = 833/845 (98.58%), Postives = 833/845 (98.58%), Query Frame = 3 
Query:   75 MIDQGTNDSYNDCHNVLDFDDSDVLMSNNFDTHSCKENNNIKNGEMQSNIYGQIERLSNEKVLIQNQCNSKMIDLQAQIMKLTNNFERIETSKTELEVELTKCHKDLRQTQRNFQIKSNELENLKIEFSNKLNSLESDYMNKISHANNLNKSLQEQICEEKRNNKNNQDFLSDKINQMFSKIKEKDEELSNNLTIQNHLQKTLTELEQQISHLTKKIEESDHMNAFHQQELHEKKEQIESLRNSELKLKSLVTELTRKVTVVESKMFEEKHELNESKSYXXXXXXXXXXXXXXXVIKEKEYNILLDRITISNKKIKELEEALSNQMQISSNYHEHLKKFENKQKIQSIDIKDQNENLARKVAQLEETIKIQSRIIEETNEELSTSRECEGTAVSNLNLTLEELDKLIEEPKMYLKSPGKSRVIGSPKIVTEFLNDSFLSPSNSCLLAKSGDHCLSPTPNKRIIRNVKQIIRDFQEHIRSLDNDKLEGIDKIQTQDKEIKNLKIQVRKYTTQNKALEIDNTEKQSEIENLTEEISKLKSLIASLKIENAKNKTNYKENVENLKSSIVRVSEESQNNIESRDKYLQNISMIIKEALQNNFTSYLDWPKFYGCRNSNFTKSNLESAFNESATQTHFADNIAKENLELTGNMTGTFCFSNQLNPPPTTTFETNLKPVEKRSRIHENNHDKKAENRIITVNDERKKSAKKVPRLPDGKKDPYVDSAQSWPALREVISNELSALLGRCDIQRAQ------------CHNLMSELSTKENIIARLLAYQNSSSFNNQGPVDTTSQCPNENAVEIPTNNNKTLIVKPNFSNQNNQTRSNIDLKKKTKVSLKSR 2573
            MIDQGTNDSYNDCHNVLDFDDSDVLMSNNFDTHSCKENNNIKNGEMQSNIYGQIERLSNEKVLIQNQCNSKMIDLQAQIMKLTNNFERIETSKTELEVELTKCHKDLRQTQRNFQIKSNELENLKIEFSNKLNSLESDYMNKISHANNLNKSLQEQICEEKRNNKNNQDFLSDKINQMFSKIKEKDEELSNNLTIQNHLQKTLTELEQQISHLTKKIEESDHMNAFHQQELHEKKEQIESLRNSELKLKSLVTELTRKVTVVESKMFEEKHELNESKSYQNNLQAQLLDLQQAQVIKEKEYNILLDRITISNKKIKELEEALSNQMQISSNYHEHLKKFENKQKIQSIDIKDQNENLARKVAQLEETIKIQSRIIEETNEELSTSRECEGTAVSNLNLTLEELDKLIEEPKMYLKSPGKSRVIGSPKIVTEFLNDSFLSPSNSCLLAKSGDHCLSPTPNKRIIRNVKQIIRDFQEHIRSLDNDKLEGIDKIQTQDKEIKNLKIQVRKYTTQNKALEIDNTEKQSEIENLTEEISKLKSLIASLKIENAKNKTNYKENVENLKSSIVRVSEESQNNIESRDKYLQNISMIIKEALQNNFTSYLDWPKFYGCRNSNFTKSNLESAFNESATQTHFADNIAKENLELTGNMTGTFCFSNQLNPPPTTTFETNLKPVEKRSRIHENNHDKKAENRIITVNDERKKSAKKVPRLPDGKKDPYVDSAQSWPALREVISNELSALLGRCDIQRAQ            CHNLMSELSTKENIIARLLAYQNSSSFNNQGPVDTTSQCPNENAVEIPTNNNKTLIVKPNFSNQNNQTRSNIDLKKKTKVSLKSR
Sbjct:    1 MIDQGTNDSYNDCHNVLDFDDSDVLMSNNFDTHSCKENNNIKNGEMQSNIYGQIERLSNEKVLIQNQCNSKMIDLQAQIMKLTNNFERIETSKTELEVELTKCHKDLRQTQRNFQIKSNELENLKIEFSNKLNSLESDYMNKISHANNLNKSLQEQICEEKRNNKNNQDFLSDKINQMFSKIKEKDEELSNNLTIQNHLQKTLTELEQQISHLTKKIEESDHMNAFHQQELHEKKEQIESLRNSELKLKSLVTELTRKVTVVESKMFEEKHELNESKSYQNNLQAQLLDLQQAQVIKEKEYNILLDRITISNKKIKELEEALSNQMQISSNYHEHLKKFENKQKIQSIDIKDQNENLARKVAQLEETIKIQSRIIEETNEELSTSRECEGTAVSNLNLTLEELDKLIEEPKMYLKSPGKSRVIGSPKIVTEFLNDSFLSPSNSCLLAKSGDHCLSPTPNKRIIRNVKQIIRDFQEHIRSLDNDKLEGIDKIQTQDKEIKNLKIQVRKYTTQNKALEIDNTEKQSEIENLTEEISKLKSLIASLKIENAKNKTNYKENVENLKSSIVRVSEESQNNIESRDKYLQNISMIIKEALQNNFTSYLDWPKFYGCRNSNFTKSNLESAFNESATQTHFADNIAKENLELTGNMTGTFCFSNQLNPPPTTTFETNLKPVEKRSRIHENNHDKKAENRIITVNDERKKSAKKVPRLPDGKKDPYVDSAQSWPALREVISNELSALLGRCDIQRAQCRIVPNEAINVNCHNLMSELSTKENIIARLLAYQNSSSFNNQGPVDTTSQCPNENAVEIPTNNNKTLIVKPNFSNQNNQTRSNIDLKKKTKVSLKSR 845

HSP 2 Score: 421.009 bits (1081), Expect = 2.281e-127
Identity = 200/201 (99.50%), Postives = 201/201 (100.00%), Query Frame = 1 
Query: 2617 KWKINYPLLMHLVVVAIMKPDTNSQMNRIGKFAHTNKRATTISTKIPERSIGSPSNNNPTMIQLPTKSSVNNLCVTKPIIISLPSTPPVTSRCRKTVNDESPQFNELIVDQRLTALILGTLINVIHLRSELETNKSLIKFYKLLYLRSVDCSQLISVNEHLECMISRSHLHSRHVNVQRKPKRFRIYAHVVMACCYISKRL 3219
            +WKINYPLLMHLVVVAIMKPDTNSQMNRIGKFAHTNKRATTISTKIPERSIGSPSNNNPTMIQLPTKSSVNNLCVTKPIIISLPSTPPVTSRCRKTVNDESPQFNELIVDQRLTALILGTLINVIHLRSELETNKSLIKFYKLLYLRSVDCSQLISVNEHLECMISRSHLHSRHVNVQRKPKRFRIYAHVVMACCYISKRL
Sbjct:  845 RWKINYPLLMHLVVVAIMKPDTNSQMNRIGKFAHTNKRATTISTKIPERSIGSPSNNNPTMIQLPTKSSVNNLCVTKPIIISLPSTPPVTSRCRKTVNDESPQFNELIVDQRLTALILGTLINVIHLRSELETNKSLIKFYKLLYLRSVDCSQLISVNEHLECMISRSHLHSRHVNVQRKPKRFRIYAHVVMACCYISKRL 1045
The following BLAST results are available for this feature:
BLAST of SMED30000998 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000998 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000998 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000998 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000998 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000998 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000998 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000998 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000998 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000998 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000998 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000998 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000998 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000998 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000008901.10.000e+098.74SMESG000008901.1[more]
SMESG000008901.11.578e-13798.16SMESG000008901.1[more]
SMESG000008901.12.281e-12798.58SMESG000008901.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30000998 ID=SMED30000998|Name=SMED30000998|organism=Schmidtea mediterranea sexual|type=transcript|length=4090bp
TTACAGCATTTGCAGCGGTAAATTGAATACTTAGAAACCTGAAGGATATC
CGATAATTGTAAAAATTTTCTTCAATGATAGACCAAGGAACAAATGATTC
TTATAATGATTGTCATAACGTCTTAGATTTTGATGATTCAGATGTTTTAA
TGTCAAACAATTTTGATACACATTCTTGTAAAGAAAACAACAATATAAAA
AATGGAGAAATGCAATCTAATATTTATGGTCAAATAGAGCGACTTTCGAA
TGAAAAGGTATTAATTCAAAATCAATGCAATTCGAAAATGATTGATTTGC
AAGCTCAAATAATGAAGTTAACAAACAATTTTGAACGAATTGAAACATCT
AAAACCGAATTAGAGGTAGAATTAACAAAGTGTCACAAAGATTTACGCCA
AACTCAAAGAAATTTTCAAATAAAATCCAATGAACTTGAAAATCTTAAAA
TCGAATTTTCTAACAAACTCAACTCACTGGAGTCTGATTACATGAACAAG
ATTTCCCATGCCAATAATTTAAACAAATCTCTTCAAGAGCAAATCTGTGA
AGAAAAACGAAACAATAAAAACAATCAAGATTTTCTATCTGATAAAATAA
ATCAAATGTTCTCGAAAATCAAAGAAAAAGACGAAGAACTTTCTAATAAT
CTCACAATTCAAAACCATCTCCAGAAAACGTTAACGGAACTGGAGCAGCA
AATATCTCATCTTACAAAGAAGATTGAAGAATCCGATCATATGAACGCAT
TCCATCAGCAAGAACTGCACGAGAAAAAGGAACAAATTGAATCACTTAGG
AATTCGGAATTGAAATTGAAAAGCCTCGTGACAGAGTTGACCCGAAAGGT
TACTGTGGTAGAGTCGAAAATGTTCGAGGAAAAACACGAATTGAATGAAT
CAAAAAGCTATCAAAACAATTTACAAGCCCAGCTACTCGATCTGCAACAA
GCTCAAGTGATAAAGGAGAAGGAATATAATATTTTGCTGGACAGAATCAC
TATTTCGAATAAAAAAATTAAAGAACTCGAAGAAGCATTAAGCAATCAAA
TGCAGATCTCTTCCAATTATCATGAACATCTTAAAAAATTCGAAAACAAA
CAGAAAATTCAGAGCATTGATATAAAAGATCAAAATGAAAATCTGGCTAG
GAAAGTAGCTCAACTCGAAGAGACGATAAAAATTCAAAGCCGAATTATTG
AAGAAACAAATGAAGAATTATCAACATCTAGGGAATGTGAAGGTACCGCT
GTATCAAATCTAAATTTGACTCTGGAAGAACTAGATAAATTAATTGAGGA
ACCGAAAATGTATTTGAAATCCCCAGGAAAATCTCGAGTTATCGGTTCGC
CAAAGATTGTTACAGAATTCCTCAATGATTCCTTCCTTTCGCCGTCTAAT
AGTTGCTTACTTGCCAAATCTGGAGATCATTGTTTAAGTCCTACGCCAAA
TAAAAGAATTATTAGAAATGTCAAACAGATTATCCGAGATTTTCAAGAGC
ACATAAGATCATTGGATAATGACAAACTGGAAGGTATTGATAAAATACAA
ACACAAGATAAAGAGATAAAGAACCTAAAAATACAAGTTCGCAAATATAC
GACTCAAAACAAAGCTCTAGAAATCGATAATACAGAAAAGCAAAGTGAGA
TTGAAAACCTGACAGAAGAAATTTCAAAGTTAAAATCTCTTATTGCTTCT
TTAAAAATTGAAAATGCTAAAAATAAGACAAATTATAAAGAAAATGTTGA
AAATCTCAAAAGTTCCATAGTTCGGGTATCGGAAGAATCTCAAAACAATA
TCGAAAGTCGAGATAAATATTTGCAAAACATATCAATGATAATAAAAGAA
GCTCTTCAGAACAACTTTACTTCCTATTTAGACTGGCCAAAATTCTATGG
TTGTCGCAATTCTAATTTCACCAAAAGTAATTTGGAAAGCGCATTTAATG
AATCTGCTACTCAGACTCATTTCGCTGATAATATAGCAAAGGAGAATTTA
GAATTAACTGGCAATATGACTGGGACATTCTGTTTCAGTAATCAGTTGAA
TCCTCCTCCTACTACTACATTTGAAACCAATTTGAAACCAGTTGAAAAAC
GCTCTCGTATTCATGAAAACAATCATGATAAAAAAGCAGAAAATCGTATC
ATAACAGTCAATGATGAAAGAAAAAAATCTGCAAAAAAGGTTCCACGACT
TCCTGATGGTAAAAAAGACCCCTATGTGGATTCGGCACAAAGTTGGCCAG
CACTTAGAGAAGTGATTTCTAATGAATTATCTGCTTTATTAGGCCGATGC
GATATTCAAAGAGCGCAGTGCCATAATTTAATGTCAGAACTGTCTACGAA
AGAGAATATTATTGCGCGCTTGTTAGCATATCAGAATTCTAGTTCTTTCA
ATAATCAAGGACCTGTTGATACAACATCTCAGTGCCCGAATGAAAACGCT
GTCGAAATCCCAACTAATAACAATAAGACATTGATTGTAAAACCCAACTT
TTCCAATCAAAATAATCAGACCAGGAGCAATATTGACTTAAAAAAGAAAA
CAAAAGTGTCATTGAAATCTCGGGTTGATAACATGTCATCTAACACATCG
AATAAACGATGTAATAAAGTGGAAAATCAACTATCCCTTACTAATGCACC
TTGTTGTTGTGGCGATAATGAAACCAGATACCAATTCACAAATGAACCGG
ATCGGAAAATTTGCACATACAAACAAGAGAGCAACAACAATATCCACCAA
AATCCCAGAAAGATCAATTGGAAGTCCATCGAACAACAACCCAACAATGA
TCCAGTTACCAACGAAATCATCAGTGAACAACTTGTGTGTGACGAAACCG
ATTATAATCTCACTTCCGAGTACGCCGCCAGTGACTTCGAGGTGTCGAAA
GACTGTTAATGATGAATCTCCACAATTTAATGAACTAATCGTTGATCAAC
GTTTGACTGCGTTAATTTTAGGAACTCTTATCAATGTGATTCATTTGCGT
TCTGAATTGGAAACCAATAAAAGTTTGATAAAATTTTATAAATTGTTGTA
TCTCCGATCTGTTGATTGTTCTCAGTTGATTTCAGTAAATGAGCATCTCG
AATGCATGATAAGTCGATCGCATTTACATTCTAGACACGTAAATGTGCAA
CGGAAACCTAAAAGGTTCAGAATTTACGCTCATGTCGTGATGGCTTGTTG
TTATATTTCCAAACGATTGTTAGCTTCCAAATTGATCATTTGCACAGATC
CTGAAGTTATGATTTCAGATGACCTTAAGGGGTTTGTCCATAATGTGATA
AATAGTGTTCCTGATTCTGTAATTATTTTGGAACTTGGATTTGATTTTAT
TATTTTTCCGGATTCAGTTTGATTGTGATCTCGTTTCTAAGGATTTTTTA
GACTCGATTTTGAATGATTTCGTTAATTTCTGTTTTGATCACAATAATAG
ATATTTGTTAGTATCAAAACCATCAAAATGCGTAACAAGTAATATTCGTG
TGTCTTCCAATACGATTGTTCTTGATAAATTAAAATCAAATGTTCATACC
ATCCAAACCATCATAGAAACTCAAATATCGAAAAAATTTTACATGTCAAA
AAAGAAATTGTATCAAAATGATGTACAAACGTTAATTGTCAAGATCAAAG
AAATGGGTCAAATTATTGATGTTCAAAATTTGGTTATTGAACAGCGACAA
AACTCAATAGTCCAACTTGAAGAAAAATTAATTCAACAAAATGATGAAAA
CTTGCACCTTATAAGCTCAATGAGACAAGTTGAAAGTTTAAATCAACTCC
AAATTCTAGAAATTGAAAGTCTGAAATTATTAATCGACAAAACGAAAGCA
TATTTAATTGCACTGGTAGAAATGTGCAATCAATATTCAAACTGTGAAAT
ATTTAATTGTGCACAACTGATGGAGGAAGAGGCATCAATATTCCACAGCA
TTATATCCGATGATTGCAATGATTTTATTAATTTTGTGAGAAACGGAATA
AGAGCAGCCTTGCGATTGTCTTGTTCTGTAAAAAGTATTTTTATTTGATT
ATTGTAGCAAATTATAAAATAAAATTCATATCTATCCTAG
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protein sequence of SMED30000998-orf-1

>SMED30000998-orf-1 ID=SMED30000998-orf-1|Name=SMED30000998-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=152bp
MSKKKLYQNDVQTLIVKIKEMGQIIDVQNLVIEQRQNSIVQLEEKLIQQN
DENLHLISSMRQVESLNQLQILEIESLKLLIDKTKAYLIALVEMCNQYSN
CEIFNCAQLMEEEASIFHSIISDDCNDFINFVRNGIRAALRLSCSVKSIF
I*
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protein sequence of SMED30000998-orf-2

>SMED30000998-orf-2 ID=SMED30000998-orf-2|Name=SMED30000998-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=243bp
MHLVVVAIMKPDTNSQMNRIGKFAHTNKRATTISTKIPERSIGSPSNNNP
TMIQLPTKSSVNNLCVTKPIIISLPSTPPVTSRCRKTVNDESPQFNELIV
DQRLTALILGTLINVIHLRSELETNKSLIKFYKLLYLRSVDCSQLISVNE
HLECMISRSHLHSRHVNVQRKPKRFRIYAHVVMACCYISKRLLASKLIIC
TDPEVMISDDLKGFVHNVINSVPDSVIILELGFDFIIFPDSV*
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protein sequence of SMED30000998-orf-3

>SMED30000998-orf-3 ID=SMED30000998-orf-3|Name=SMED30000998-orf-3|organism=Schmidtea mediterranea sexual|type=polypeptide|length=945bp
MIDQGTNDSYNDCHNVLDFDDSDVLMSNNFDTHSCKENNNIKNGEMQSNI
YGQIERLSNEKVLIQNQCNSKMIDLQAQIMKLTNNFERIETSKTELEVEL
TKCHKDLRQTQRNFQIKSNELENLKIEFSNKLNSLESDYMNKISHANNLN
KSLQEQICEEKRNNKNNQDFLSDKINQMFSKIKEKDEELSNNLTIQNHLQ
KTLTELEQQISHLTKKIEESDHMNAFHQQELHEKKEQIESLRNSELKLKS
LVTELTRKVTVVESKMFEEKHELNESKSYQNNLQAQLLDLQQAQVIKEKE
YNILLDRITISNKKIKELEEALSNQMQISSNYHEHLKKFENKQKIQSIDI
KDQNENLARKVAQLEETIKIQSRIIEETNEELSTSRECEGTAVSNLNLTL
EELDKLIEEPKMYLKSPGKSRVIGSPKIVTEFLNDSFLSPSNSCLLAKSG
DHCLSPTPNKRIIRNVKQIIRDFQEHIRSLDNDKLEGIDKIQTQDKEIKN
LKIQVRKYTTQNKALEIDNTEKQSEIENLTEEISKLKSLIASLKIENAKN
KTNYKENVENLKSSIVRVSEESQNNIESRDKYLQNISMIIKEALQNNFTS
YLDWPKFYGCRNSNFTKSNLESAFNESATQTHFADNIAKENLELTGNMTG
TFCFSNQLNPPPTTTFETNLKPVEKRSRIHENNHDKKAENRIITVNDERK
KSAKKVPRLPDGKKDPYVDSAQSWPALREVISNELSALLGRCDIQRAQCH
NLMSELSTKENIIARLLAYQNSSSFNNQGPVDTTSQCPNENAVEIPTNNN
KTLIVKPNFSNQNNQTRSNIDLKKKTKVSLKSRVDNMSSNTSNKRCNKVE
NQLSLTNAPCCCGDNETRYQFTNEPDRKICTYKQESNNNIHQNPRKINWK
SIEQQPNNDPVTNEIISEQLVCDETDYNLTSEYAASDFEVSKDC*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000418head
PLANA:0002089reproductive organ
Vocabulary: molecular function
TermDefinition
GO:0046872metal ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 72..138
NoneNo IPR availableCOILSCoilCoilcoord: 301..321
NoneNo IPR availableCOILSCoilCoilcoord: 491..546
NoneNo IPR availableCOILSCoilCoilcoord: 551..571
NoneNo IPR availableCOILSCoilCoilcoord: 347..381
NoneNo IPR availableCOILSCoilCoilcoord: 196..244
NoneNo IPR availablePANTHERPTHR34491FAMILY NOT NAMEDcoord: 53..853