tRNA (Uracil-5-)-methyltransferase homolog A
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30000955 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 4
Homology
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Human
Match: TRMT2A (tRNA methyltransferase 2 homolog A [Source:HGNC Symbol;Acc:HGNC:24974]) HSP 1 Score: 306.605 bits (784), Expect = 1.703e-95 Identity = 183/521 (35.12%), Postives = 262/521 (50.29%), Query Frame = 1
Query: 220 KRDEAISKVTGHNWKGNVLLAKVAKGRADPFLQKRKEMENVTEISDCKKIKLENDDISNEEKLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKENILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIG---DTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTRRKNNSDP-LDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYNNFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTRL 1770
+RD+A+ + G WKG L ++A+ +ADP ++R++ E + ++ D V W PY QL K+ + + +LQ + +I N + + N CPL + PSP YRNKCEF +G ++ G GKY+GG A+ DT +P Q+ K P S + P T GH + +T+RT + + I HPQ LS EL + A++F G G+ VT L P + + + G ++E L+ L FRIS +AFFQVNT AAE LY+ I+ +D+ S +LDVCCGTGTIGL +A V +VIGVE+CPE+VEDA N + N ++ FH G+AED++ L+S + VAI+DPPRAGLH VI A+RR + ++++++VSCNP AA NF+D R S R G PF P AV VDLFP TPH E+++L R+
Sbjct: 121 ERDKALRVLHGALWKGRPLSVRLARPKADPMARRRRQ---------------EGESEPPVTRVADVVTPLWTVPYAEQLERKQLECEQVLQKLAKEIGSTNRALLPWL-LEQRHKHNKACCPLEGVRPSPQQTEYRNKCEFLVGVGVDGEDNTVGCRLGKYKGGTCAVAAPFDTVHIPEATKQVVKAFQEFIRSTP----------YSAYDPETYTGHWKQLTVRTSRRH-------------QAMAIAYFHPQKLSPEELAELKTSLAQHFTAGPGRASGVTCLYFVEEGQRKTPSQEGLPLEHVAGDRCIHEDLLGLTFRISPHAFFQVNTPAAEVLYTVIQDWA---QLDAGS----MVLDVCCGTGTIGLALARKVKRVIGVELCPEAVEDARVNAQDNELSNVEFHCGRAEDLVPTLVSRLAS------QHLVAILDPPRAGLHSKVILAIRRAKNLRRLLYVSCNPRAAMGNFVDLCRAPSNRVKGIPFRPVKAVAVDLFPQTPHCEMLILFERV 589 HSP 2 Score: 51.9878 bits (123), Expect = 2.630e-6 Identity = 24/59 (40.68%), Postives = 38/59 (64.41%), Query Frame = 3
Query: 54 YGYLDKGEFTSELFKIEIKNLHPKVGFSQLKQRLKSIGLNPHKVKITNE--CSFVTFKT 224
Y Y+ FTSE+FK+E++N+ FS +++ L GL PHK K+ + C+FVTF++
Sbjct: 60 YSYIRDDLFTSEIFKLELQNVPRHASFSDVRRFLGRFGLQPHKTKLFGQPPCAFVTFRS 118
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Human
Match: TRMT2A (tRNA methyltransferase 2 homolog A [Source:HGNC Symbol;Acc:HGNC:24974]) HSP 1 Score: 306.605 bits (784), Expect = 1.703e-95 Identity = 183/521 (35.12%), Postives = 262/521 (50.29%), Query Frame = 1
Query: 220 KRDEAISKVTGHNWKGNVLLAKVAKGRADPFLQKRKEMENVTEISDCKKIKLENDDISNEEKLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKENILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIG---DTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTRRKNNSDP-LDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYNNFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTRL 1770
+RD+A+ + G WKG L ++A+ +ADP ++R++ E + ++ D V W PY QL K+ + + +LQ + +I N + + N CPL + PSP YRNKCEF +G ++ G GKY+GG A+ DT +P Q+ K P S + P T GH + +T+RT + + I HPQ LS EL + A++F G G+ VT L P + + + G ++E L+ L FRIS +AFFQVNT AAE LY+ I+ +D+ S +LDVCCGTGTIGL +A V +VIGVE+CPE+VEDA N + N ++ FH G+AED++ L+S + VAI+DPPRAGLH VI A+RR + ++++++VSCNP AA NF+D R S R G PF P AV VDLFP TPH E+++L R+
Sbjct: 121 ERDKALRVLHGALWKGRPLSVRLARPKADPMARRRRQ---------------EGESEPPVTRVADVVTPLWTVPYAEQLERKQLECEQVLQKLAKEIGSTNRALLPWL-LEQRHKHNKACCPLEGVRPSPQQTEYRNKCEFLVGVGVDGEDNTVGCRLGKYKGGTCAVAAPFDTVHIPEATKQVVKAFQEFIRSTP----------YSAYDPETYTGHWKQLTVRTSRRH-------------QAMAIAYFHPQKLSPEELAELKTSLAQHFTAGPGRASGVTCLYFVEEGQRKTPSQEGLPLEHVAGDRCIHEDLLGLTFRISPHAFFQVNTPAAEVLYTVIQDWA---QLDAGS----MVLDVCCGTGTIGLALARKVKRVIGVELCPEAVEDARVNAQDNELSNVEFHCGRAEDLVPTLVSRLAS------QHLVAILDPPRAGLHSKVILAIRRAKNLRRLLYVSCNPRAAMGNFVDLCRAPSNRVKGIPFRPVKAVAVDLFPQTPHCEMLILFERV 589 HSP 2 Score: 51.9878 bits (123), Expect = 2.630e-6 Identity = 24/59 (40.68%), Postives = 38/59 (64.41%), Query Frame = 3
Query: 54 YGYLDKGEFTSELFKIEIKNLHPKVGFSQLKQRLKSIGLNPHKVKITNE--CSFVTFKT 224
Y Y+ FTSE+FK+E++N+ FS +++ L GL PHK K+ + C+FVTF++
Sbjct: 60 YSYIRDDLFTSEIFKLELQNVPRHASFSDVRRFLGRFGLQPHKTKLFGQPPCAFVTFRS 118
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Human
Match: TRMT2A (tRNA methyltransferase 2 homolog A [Source:HGNC Symbol;Acc:HGNC:24974]) HSP 1 Score: 295.434 bits (755), Expect = 4.189e-91 Identity = 183/539 (33.95%), Postives = 262/539 (48.61%), Query Frame = 1
Query: 220 KRDEAISKVTGHNWKGNVLLAKVAKGRADPFLQKRKEMENVTEISDCKKIKLENDDISNEEKLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKENILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIG---DTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTRRKNNSDP-LDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMA------------------DLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYNNFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTRL 1770
+RD+A+ + G WKG L ++A+ +ADP ++R++ E + ++ D V W PY QL K+ + + +LQ + +I N + + N CPL + PSP YRNKCEF +G ++ G GKY+GG A+ DT +P Q+ K P S + P T GH + +T+RT + + I HPQ LS EL + A++F G G+ VT L P + + + G ++E L+ L FRIS +AFFQVNT AAE LY+ I+ +D+ S +LDVCCGTGTIGL +A V +VIGVE+CPE+VEDA N + N ++ FH G+AED++ L+S + VAI+DPPRAGLH VI A+RR + ++++++VSCNP AA NF+D R S R G PF P AV VDLFP TPH E+++L R+
Sbjct: 121 ERDKALRVLHGALWKGRPLSVRLARPKADPMARRRRQ---------------EGESEPPVTRVADVVTPLWTVPYAEQLERKQLECEQVLQKLAKEIGSTNRALLPWL-LEQRHKHNKACCPLEGVRPSPQQTEYRNKCEFLVGVGVDGEDNTVGCRLGKYKGGTCAVAAPFDTVHIPEATKQVVKAFQEFIRSTP----------YSAYDPETYTGHWKQLTVRTSRRH-------------QAMAIAYFHPQKLSPEELAELKTSLAQHFTAGPGRASGVTCLYFVEEGQRKTPSQEGLPLEHVAGDRCIHEDLLGLTFRISPHAFFQVNTPAAEVLYTVIQDWA---QLDAGS----MVLDVCCGTGTIGLALARGPMYSPPWVGRHHAFLFQKVKRVIGVELCPEAVEDARVNAQDNELSNVEFHCGRAEDLVPTLVSRLAS------QHLVAILDPPRAGLHSKVILAIRRAKNLRRLLYVSCNPRAAMGNFVDLCRAPSNRVKGIPFRPVKAVAVDLFPQTPHCEMLILFERV 607 HSP 2 Score: 51.9878 bits (123), Expect = 2.687e-6 Identity = 24/59 (40.68%), Postives = 38/59 (64.41%), Query Frame = 3
Query: 54 YGYLDKGEFTSELFKIEIKNLHPKVGFSQLKQRLKSIGLNPHKVKITNE--CSFVTFKT 224
Y Y+ FTSE+FK+E++N+ FS +++ L GL PHK K+ + C+FVTF++
Sbjct: 60 YSYIRDDLFTSEIFKLELQNVPRHASFSDVRRFLGRFGLQPHKTKLFGQPPCAFVTFRS 118
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Human
Match: TRMT2A (tRNA methyltransferase 2 homolog A [Source:HGNC Symbol;Acc:HGNC:24974]) HSP 1 Score: 268.085 bits (684), Expect = 1.583e-81 Identity = 163/481 (33.89%), Postives = 238/481 (49.48%), Query Frame = 1
Query: 220 KRDEAISKVTGHNWKGNVLLAKVAKGRADPFLQKRKEMENVTEISDCKKIKLENDDISNEEKLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKENILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIG---DTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTRRKNNSDP-LDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYNNFID 1650
+RD+A+ + G WKG L ++A+ +ADP ++R++ E + ++ D V W PY QL K+ + + +LQ + +I N + + N CPL + PSP YRNKCEF +G ++ G GKY+GG A+ DT +P Q+ K P S + P T GH + +T+RT + + I HPQ LS EL + A++F G G+ VT L P + + + G ++E L+ L FRIS +AFFQVNT AAE LY+ I+ +D+ S +LDVCCGTGTIGL +A V +VIGVE+CPE+VEDA N + N ++ FH G+AED++ L+S + VAI+DPPRAGLH VI A+RR + ++++++VSCNP AA NF+D
Sbjct: 121 ERDKALRVLHGALWKGRPLSVRLARPKADPMARRRRQ---------------EGESEPPVTRVADVVTPLWTVPYAEQLERKQLECEQVLQKLAKEIGSTNRALLPWL-LEQRHKHNKACCPLEGVRPSPQQTEYRNKCEFLVGVGVDGEDNTVGCRLGKYKGGTCAVAAPFDTVHIPEATKQVVKAFQEFIRSTP----------YSAYDPETYTGHWKQLTVRTSRRH-------------QAMAIAYFHPQKLSPEELAELKTSLAQHFTAGPGRASGVTCLYFVEEGQRKTPSQEGLPLEHVAGDRCIHEDLLGLTFRISPHAFFQVNTPAAEVLYTVIQDWA---QLDAGS----MVLDVCCGTGTIGLALARKVKRVIGVELCPEAVEDARVNAQDNELSNVEFHCGRAEDLVPTLVSRLAS------QHLVAILDPPRAGLHSKVILAIRRAKNLRRLLYVSCNPRAAMGNFVD 549 HSP 2 Score: 51.9878 bits (123), Expect = 2.561e-6 Identity = 24/59 (40.68%), Postives = 38/59 (64.41%), Query Frame = 3
Query: 54 YGYLDKGEFTSELFKIEIKNLHPKVGFSQLKQRLKSIGLNPHKVKITNE--CSFVTFKT 224
Y Y+ FTSE+FK+E++N+ FS +++ L GL PHK K+ + C+FVTF++
Sbjct: 60 YSYIRDDLFTSEIFKLELQNVPRHASFSDVRRFLGRFGLQPHKTKLFGQPPCAFVTFRS 118
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Human
Match: TRMT2B (tRNA methyltransferase 2 homolog B [Source:HGNC Symbol;Acc:HGNC:25748]) HSP 1 Score: 259.61 bits (662), Expect = 6.255e-79 Identity = 167/464 (35.99%), Postives = 234/464 (50.43%), Query Frame = 1
Query: 385 DISNEEKLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKENILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIGDTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTRRKNNSDPLDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEA-AYNNFIDFARP--ISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTR 1767
D S +E+L D V WR Y+ QL K K ILQ + I N + + + + C L PIIPSPV + YRNK F++ + GF G +R G ++ L IP + S+V + L Q P L VF G G+ R +T+RT S + II HPQ LS+ EL + E+F+ G G C +TSL + + +FG +++E+L++L RIS +AFFQ+NT AE LY T+ +L + LLD+CCGTG IGL +A +V+G+E+ ++VEDA + N IT + FH G+AE ++ LL + E Q VA+V+P RAGLH VIQA+R I ++FVSC + N I+ P +K+ G PF+ + AVPVDLFPHTPH ELV+L TR
Sbjct: 77 DGSWQERLADVVTPLWRLSYEEQLKVKFAAQKKILQRLESYIQMLNGVSVTT----AVPKSERLSCLLHPIIPSPVINGYRNKSTFSVNRGPDGNPKTVGFYLGTWRDGNVVCVQSNHLKNIPEKHSQVAQYYEVFLRQ---SPLEPCL-VFHEG---GYWRELTVRTNSQG-------------HTMAIITFHPQKLSQEELHVQKEIVKEFFIRGPGAACGLTSLYFQESTMTRCSHQQSPYQLLFGEPYIFEELLSLKIRISPDAFFQINTAGAEMLYRTVGEL-------TGVNSDTILLDICCGTGVIGLSLAQHTSRVLGIELLEQAVEDARWTAAFNGITNSEFHTGQAEKILPGLLKSKEDGQ-----SIVAVVNPARAGLHYKVIQAIRNFRAIHTLVFVSCKLHGESTRNVIELCCPPDPAKKLLGEPFVLQQAVPVDLFPHTPHCELVLLFTR 504
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Celegans
Match: trm-2A (TRna (tRNA) Methyltransferase homolog [Source:UniProtKB/TrEMBL;Acc:Q9N5L0]) HSP 1 Score: 122.865 bits (307), Expect = 5.253e-31 Identity = 67/165 (40.61%), Postives = 94/165 (56.97%), Query Frame = 1
Query: 1291 LLDVCCGTGTIGLVMADLVDK-----VIGVEMCPESVEDANYNKELNNI-TKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYNNFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTR 1767
LLD+CCGTGTIG + + K IG+EM E+VEDA N + N + T + AGKAED ++ + V ++DPPRAG+H VI A R E ++++IFVSC P AA +N ++ RP S++ G F PVD+FP T H+E ++ L R
Sbjct: 121 LLDICCGTGTIGQCLLKNIQKSQKVCCIGIEMIAEAVEDAKQNAKQNKMETCCKYIAGKAEDTFRSMRYHLPNGFDLRKSRVVGVLDPPRAGMHEKVILACREMETMRRLIFVSCEPNAAISNIVNLCRPSSRKYTGDAFSVTRVQPVDMFPQTNHMEWIIQLDR 285 HSP 2 Score: 49.2914 bits (116), Expect = 4.185e-6 Identity = 22/45 (48.89%), Postives = 29/45 (64.44%), Query Frame = 1
Query: 1102 DNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTI 1236
D K I G+ ++YE L++ FR+S AFFQ N+ AA LYSTI
Sbjct: 6 DTPNFKLIGGTPYIYESLLDCRFRVSPPAFFQTNSQAAAVLYSTI 50
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Celegans
Match: trm-2A (TRna (tRNA) Methyltransferase homolog [Source:UniProtKB/TrEMBL;Acc:Q9N5L0]) HSP 1 Score: 124.02 bits (310), Expect = 1.150e-29 Identity = 67/165 (40.61%), Postives = 94/165 (56.97%), Query Frame = 1
Query: 1291 LLDVCCGTGTIGLVMADLVDK-----VIGVEMCPESVEDANYNKELNNI-TKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYNNFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTR 1767
LLD+CCGTGTIG + + K IG+EM E+VEDA N + N + T + AGKAED ++ + V ++DPPRAG+H VI A R E ++++IFVSC P AA +N ++ RP S++ G F PVD+FP T H+E ++ L R
Sbjct: 439 LLDICCGTGTIGQCLLKNIQKSQKVCCIGIEMIAEAVEDAKQNAKQNKMETCCKYIAGKAEDTFRSMRYHLPNGFDLRKSRVVGVLDPPRAGMHEKVILACREMETMRRLIFVSCEPNAAISNIVNLCRPSSRKYTGDAFSVTRVQPVDMFPQTNHMEWIIQLDR 603 HSP 2 Score: 86.6557 bits (213), Expect = 9.133e-20 Identity = 84/338 (24.85%), Postives = 148/338 (43.79%), Query Frame = 1
Query: 229 EAISKVTGHNWKGNVLLAKVAKGRADPFLQKRKEMENVTEISDCKKIKLENDDISNEEKLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKI--NKENILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIGDTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTRRKNNSDPLDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTI 1236
+AI+ V G+++K +L A++A TE+ + EN + + ++ ++ V Y QL K+ G+++ ++ ++ NK L K+++ F ++ L I+P+P T YRNKCEFTIG L N GF G++ + + I+ QM ++ +++ + + L F +G R +T+R + D+ + + + + E+Q + F T K K V S+ + + SD K I G+ ++YE L++ FR+S AFFQ N+ AA LYSTI
Sbjct: 78 KAINLVDGYDYKKFILSAQLA----------------ATEVKSFRPANPENTEPAQQKTARESVTPLAEMEYGKQLELKQQNSVGVVEKLYRQLIQNKVAHLGKQTQ-FRDRV--------LREILPAPKTAGYRNKCEFTIGHDL-NGEICVGFVGGRFSKNQHFVIPPVDVDIVSTQMMAIVGDVHEFVKE-------SALEPFNEFDRKGFWRMLTIR-----------EFGGDVMLIFTVFPLEDGVERMEEIQKKIETRFLDFETFKEKKYRVCSIYWQQMVHISD---TPNFKLIGGTPYIYESLLDCRFRVSPPAFFQTNSQAAAVLYSTI 368 HSP 3 Score: 29.6462 bits (65), Expect = 9.133e-20 Identity = 19/54 (35.19%), Postives = 32/54 (59.26%), Query Frame = 3
Query: 69 KGEFTSELFKIEIKNLHPKVGFSQLKQRLKSI--GLNPHKVKITNECSFVTFKT 224
K E +L +I IKN+ +GF Q K+ L+ I + KV+ ++ ++VTF+T
Sbjct: 19 KEEKAIDLDRIHIKNIPTFIGFKQFKKVLEKILGDVKSKKVRHMSDYAYVTFET 72
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Celegans
Match: trm-2A (TRna (tRNA) Methyltransferase homolog [Source:UniProtKB/TrEMBL;Acc:Q9N5L0]) HSP 1 Score: 124.02 bits (310), Expect = 1.150e-29 Identity = 67/165 (40.61%), Postives = 94/165 (56.97%), Query Frame = 1
Query: 1291 LLDVCCGTGTIGLVMADLVDK-----VIGVEMCPESVEDANYNKELNNI-TKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYNNFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTR 1767
LLD+CCGTGTIG + + K IG+EM E+VEDA N + N + T + AGKAED ++ + V ++DPPRAG+H VI A R E ++++IFVSC P AA +N ++ RP S++ G F PVD+FP T H+E ++ L R
Sbjct: 439 LLDICCGTGTIGQCLLKNIQKSQKVCCIGIEMIAEAVEDAKQNAKQNKMETCCKYIAGKAEDTFRSMRYHLPNGFDLRKSRVVGVLDPPRAGMHEKVILACREMETMRRLIFVSCEPNAAISNIVNLCRPSSRKYTGDAFSVTRVQPVDMFPQTNHMEWIIQLDR 603 HSP 2 Score: 86.6557 bits (213), Expect = 9.133e-20 Identity = 84/338 (24.85%), Postives = 148/338 (43.79%), Query Frame = 1
Query: 229 EAISKVTGHNWKGNVLLAKVAKGRADPFLQKRKEMENVTEISDCKKIKLENDDISNEEKLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKI--NKENILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIGDTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTRRKNNSDPLDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTI 1236
+AI+ V G+++K +L A++A TE+ + EN + + ++ ++ V Y QL K+ G+++ ++ ++ NK L K+++ F ++ L I+P+P T YRNKCEFTIG L N GF G++ + + I+ QM ++ +++ + + L F +G R +T+R + D+ + + + + E+Q + F T K K V S+ + + SD K I G+ ++YE L++ FR+S AFFQ N+ AA LYSTI
Sbjct: 78 KAINLVDGYDYKKFILSAQLA----------------ATEVKSFRPANPENTEPAQQKTARESVTPLAEMEYGKQLELKQQNSVGVVEKLYRQLIQNKVAHLGKQTQ-FRDRV--------LREILPAPKTAGYRNKCEFTIGHDL-NGEICVGFVGGRFSKNQHFVIPPVDVDIVSTQMMAIVGDVHEFVKE-------SALEPFNEFDRKGFWRMLTIR-----------EFGGDVMLIFTVFPLEDGVERMEEIQKKIETRFLDFETFKEKKYRVCSIYWQQMVHISD---TPNFKLIGGTPYIYESLLDCRFRVSPPAFFQTNSQAAAVLYSTI 368 HSP 3 Score: 29.6462 bits (65), Expect = 9.133e-20 Identity = 19/54 (35.19%), Postives = 32/54 (59.26%), Query Frame = 3
Query: 69 KGEFTSELFKIEIKNLHPKVGFSQLKQRLKSI--GLNPHKVKITNECSFVTFKT 224
K E +L +I IKN+ +GF Q K+ L+ I + KV+ ++ ++VTF+T
Sbjct: 19 KEEKAIDLDRIHIKNIPTFIGFKQFKKVLEKILGDVKSKKVRHMSDYAYVTFET 72
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Celegans
Match: trm-2A (TRna (tRNA) Methyltransferase homolog [Source:UniProtKB/TrEMBL;Acc:Q9N5L0]) HSP 1 Score: 97.8265 bits (242), Expect = 6.378e-24 Identity = 52/134 (38.81%), Postives = 75/134 (55.97%), Query Frame = 1
Query: 1369 MCPESVEDANYNKELNNI-TKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYNNFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTR 1767
M E+VEDA N + N + T + AGKAED ++ + V ++DPPRAG+H VI A R E ++++IFVSC P AA +N ++ RP S++ G F PVD+FP T H+E ++ L R
Sbjct: 1 MIAEAVEDAKQNAKQNKMETCCKYIAGKAEDTFRSMRYHLPNGFDLRKSRVVGVLDPPRAGMHEKVILACREMETMRRLIFVSCEPNAAISNIVNLCRPSSRKYTGDAFSVTRVQPVDMFPQTNHMEWIIQLDR 134
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Celegans
Match: trm-2A (TRna (tRNA) Methyltransferase homolog [Source:UniProtKB/TrEMBL;Acc:Q9N5L0]) HSP 1 Score: 57.3806 bits (137), Expect = 1.338e-10 Identity = 25/61 (40.98%), Postives = 38/61 (62.30%), Query Frame = 1
Query: 1585 ELIKKVIFVSCNPEAAYNNFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTR 1767
E ++++IFVSC P AA +N ++ RP S++ G F PVD+FP T H+E ++ L R
Sbjct: 2 ETMRRLIFVSCEPNAAISNIVNLCRPSSRKYTGDAFSVTRVQPVDMFPQTNHMEWIIQLDR 62
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Fly
Match: CG3808 (gene:FBgn0036838 transcript:FBtr0075041) HSP 1 Score: 253.062 bits (645), Expect = 1.101e-75 Identity = 174/523 (33.27%), Postives = 261/523 (49.90%), Query Frame = 1
Query: 223 RDEAISKVTGHNWKGNVLLAKVAKGRADPFLQKRKEMENVTEISDCKKIKLENDDISNEEKLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKENILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIA-GFCHGKYRGGFDAIGDTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENEL----QSIGDFFAEYFLTGKGKTCNVTSLLLTRRKNNS--DPLDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQE-NNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYNNFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTR 1767
+ A+ + G+ WKG VL A VAK ADP +KR E +E +K K + + L H+ YD Q+ K ++ +L ++ K N K + + Q ++ ++PSP + YRNK EFT+G + G + GF G Y G + + + LP +P Q D++ + +F P F P + GH R + +R S + L+++ I+ LSE+E Q + F+ + K ++ + R+ +P+++ I GS + + + L FRIS AFFQ+NT A LY Q ID V + K T+LD+CCGTGTI L A KV+GVE+ P++++DA +N E N I A F G A+D I +++ A QE + +A+VDPPRAGLH I A+R + I ++++VSCNP +A NFI+ ARP SK+ G PF P+ AV VD+FPHT H ELV+L R
Sbjct: 110 QQRALEILNGYKWKGKVLKAHVAKASADPLQKKRAAEEQESE----RKPKKQRTAVEATCPL-SHIA------YDQQIKQKSEEMSALLAKYTQELRKVNPRAKPHLDKF-QFHE---------VLPSPTVNGYRNKNEFTVG--KNSVGEVVVGFRLGCYSDGSVEVAEVADLPHLPEQAKWAARSFQDLVRK---SKFLP----FNPDGNIGHFRQLMVRCSSATGE------------LMLVAGIYSSNLSEDEQVELKQELKSFYEDLANDAPYKCSSLYYQDVKHREAGQTINPVEH-----ISGSTHITDTIQGLQFRISPLAFFQINTEGANVLY----QKAIDLVAPT---KDTTMLDICCGTGTITLAFAKHCKKVMGVEIVPDAIKDAEFNAEANGIKNAKFFTGNADDFIKSMVREALYDQEPGKPLDLIAVVDPPRAGLHHRSIAAIRSADAINRLVYVSCNPHSAKRNFIELARPESKQYKGEPFYPKSAVAVDMFPHTMHTELVILFER 578
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Zebrafish
Match: trmt2a (tRNA methyltransferase 2 homolog A [Source:ZFIN;Acc:ZDB-GENE-030131-6876]) HSP 1 Score: 334.724 bits (857), Expect = 1.568e-106 Identity = 195/524 (37.21%), Postives = 282/524 (53.82%), Query Frame = 1
Query: 220 KRDEAISKVTGHNWKGNVLLAKVAKGRADPFLQKRKEMENVTEISDCKKIKLENDDISNEE------KLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKENILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIGDTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTRR-KNNSDPLDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYNNFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTRL 1770
+RD+A+ V G WK NVL ++AK +ADP L+KRK+ E K + + EE ++ + V W+ PY+ QL+ KE +++G+LQ + +I N + F + N + CPL I PSP+ YRNKCEF IG + GF GKY+GG SC + P + + V ++ T +V+ P T +GH R +T+RT + Q++ M+ +PQ L E EL+ + +YF G+GK C VTSL R + S ++ + + G +++E+L+ L FRIS ++FFQ NT AAE LYS + + +D S T+LDVCCGTGTIG+ +A V KVIG+E+C E+V+DA N + N +T FH GKAED+ +L+ AIVDPPRAGLH VI A+R+ E +K++++V+CN +AA NNFID R S R G F P A+ VDLFP T H E ++L R+
Sbjct: 122 ERDKAMKAVHGMQWKSNVLSVRLAKPKADPILKKRKQEEEADSGQPGAKRNTGSQEAEKEEEEPPSIQIANAVTPLWKVPYEEQLMMKEKEVEGVLQKLAREIGNNNKAMLPWL-FVQKEKHNKMCCPLEAIRPSPLQTGYRNKCEFVIGVGADGEDRTVGFRLGKYKGG-------SCAVVSPSETTNVCSEAKRVVQCFQQFIRTTPYAVYSPETYEGHWRQLTVRTTRLK------------QTMAMVF-FNPQKLQEKELEELKQNLGQYFTEGEGKECAVTSLYFVRMGQRTSAGTEDLPCEHVTGEEWIHEELLGLKFRISPHSFFQTNTPAAEILYSAVGEWA---QLDQDS----TVLDVCCGTGTIGISLAKRVKKVIGIELCQEAVKDAEANAKANGLTNVEFHCGKAEDVFPTILNAVVSPN------VTAIVDPPRAGLHSKVILAIRKAEHLKRLVYVACNAKAAMNNFIDLCRAPSNRVRGAAFRPVKAMAVDLFPQTMHCETILLFERV 611 HSP 2 Score: 53.5286 bits (127), Expect = 5.153e-7 Identity = 27/60 (45.00%), Postives = 42/60 (70.00%), Query Frame = 3
Query: 54 YGYLDKGEFTSELFKIEIKNLHPKVGFSQLKQRLKSIGLNPHKVKI--TNECSFVTFKTR 227
Y Y+ + FTSE++K+EI+NL +GF+ LK+ L G+NPHK+K+ + +FVTFK +
Sbjct: 61 YRYIKEDLFTSEIYKVEIQNLPKYIGFNDLKKFLNKHGINPHKIKLLGRQKFAFVTFKNQ 120
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Zebrafish
Match: trmt2a (tRNA methyltransferase 2 homolog A [Source:ZFIN;Acc:ZDB-GENE-030131-6876]) HSP 1 Score: 334.724 bits (857), Expect = 1.568e-106 Identity = 195/524 (37.21%), Postives = 282/524 (53.82%), Query Frame = 1
Query: 220 KRDEAISKVTGHNWKGNVLLAKVAKGRADPFLQKRKEMENVTEISDCKKIKLENDDISNEE------KLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKENILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIGDTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTRR-KNNSDPLDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYNNFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTRL 1770
+RD+A+ V G WK NVL ++AK +ADP L+KRK+ E K + + EE ++ + V W+ PY+ QL+ KE +++G+LQ + +I N + F + N + CPL I PSP+ YRNKCEF IG + GF GKY+GG SC + P + + V ++ T +V+ P T +GH R +T+RT + Q++ M+ +PQ L E EL+ + +YF G+GK C VTSL R + S ++ + + G +++E+L+ L FRIS ++FFQ NT AAE LYS + + +D S T+LDVCCGTGTIG+ +A V KVIG+E+C E+V+DA N + N +T FH GKAED+ +L+ AIVDPPRAGLH VI A+R+ E +K++++V+CN +AA NNFID R S R G F P A+ VDLFP T H E ++L R+
Sbjct: 122 ERDKAMKAVHGMQWKSNVLSVRLAKPKADPILKKRKQEEEADSGQPGAKRNTGSQEAEKEEEEPPSIQIANAVTPLWKVPYEEQLMMKEKEVEGVLQKLAREIGNNNKAMLPWL-FVQKEKHNKMCCPLEAIRPSPLQTGYRNKCEFVIGVGADGEDRTVGFRLGKYKGG-------SCAVVSPSETTNVCSEAKRVVQCFQQFIRTTPYAVYSPETYEGHWRQLTVRTTRLK------------QTMAMVF-FNPQKLQEKELEELKQNLGQYFTEGEGKECAVTSLYFVRMGQRTSAGTEDLPCEHVTGEEWIHEELLGLKFRISPHSFFQTNTPAAEILYSAVGEWA---QLDQDS----TVLDVCCGTGTIGISLAKRVKKVIGIELCQEAVKDAEANAKANGLTNVEFHCGKAEDVFPTILNAVVSPN------VTAIVDPPRAGLHSKVILAIRKAEHLKRLVYVACNAKAAMNNFIDLCRAPSNRVRGAAFRPVKAMAVDLFPQTMHCETILLFERV 611 HSP 2 Score: 53.5286 bits (127), Expect = 5.153e-7 Identity = 27/60 (45.00%), Postives = 42/60 (70.00%), Query Frame = 3
Query: 54 YGYLDKGEFTSELFKIEIKNLHPKVGFSQLKQRLKSIGLNPHKVKI--TNECSFVTFKTR 227
Y Y+ + FTSE++K+EI+NL +GF+ LK+ L G+NPHK+K+ + +FVTFK +
Sbjct: 61 YRYIKEDLFTSEIYKVEIQNLPKYIGFNDLKKFLNKHGINPHKIKLLGRQKFAFVTFKNQ 120
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Zebrafish
Match: trmt2a (tRNA methyltransferase 2 homolog A [Source:ZFIN;Acc:ZDB-GENE-030131-6876]) HSP 1 Score: 334.339 bits (856), Expect = 2.353e-106 Identity = 195/524 (37.21%), Postives = 282/524 (53.82%), Query Frame = 1
Query: 220 KRDEAISKVTGHNWKGNVLLAKVAKGRADPFLQKRKEMENVTEISDCKKIKLENDDISNEE------KLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKENILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIGDTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTRR-KNNSDPLDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYNNFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTRL 1770
+RD+A+ V G WK NVL ++AK +ADP L+KRK+ E K + + EE ++ + V W+ PY+ QL+ KE +++G+LQ + +I N + F + N + CPL I PSP+ YRNKCEF IG + GF GKY+GG SC + P + + V ++ T +V+ P T +GH R +T+RT + Q++ M+ +PQ L E EL+ + +YF G+GK C VTSL R + S ++ + + G +++E+L+ L FRIS ++FFQ NT AAE LYS + + +D S T+LDVCCGTGTIG+ +A V KVIG+E+C E+V+DA N + N +T FH GKAED+ +L+ AIVDPPRAGLH VI A+R+ E +K++++V+CN +AA NNFID R S R G F P A+ VDLFP T H E ++L R+
Sbjct: 124 ERDKAMKAVHGMQWKSNVLSVRLAKPKADPILKKRKQEEEADSGQPGAKRNTGSQEAEKEEEEPPSIQIANAVTPLWKVPYEEQLMMKEKEVEGVLQKLAREIGNNNKAMLPWL-FVQKEKHNKMCCPLEAIRPSPLQTGYRNKCEFVIGVGADGEDRTVGFRLGKYKGG-------SCAVVSPSETTNVCSEAKRVVQCFQQFIRTTPYAVYSPETYEGHWRQLTVRTTRLK------------QTMAMVF-FNPQKLQEKELEELKQNLGQYFKEGEGKECAVTSLYFVRMGQRTSAGTEDLPCEHVTGEEWIHEELLGLKFRISPHSFFQTNTPAAEILYSAVGEWA---QLDQDS----TVLDVCCGTGTIGISLAKRVKKVIGIELCQEAVKDAEANAKANGLTNVEFHCGKAEDVFPTILNAVVSPN------VTAIVDPPRAGLHSKVILAIRKAEHLKRLVYVACNAKAAMNNFIDLCRAPSNRVRGAAFRPVRAMAVDLFPQTMHCETILLFERV 613 HSP 2 Score: 53.5286 bits (127), Expect = 5.251e-7 Identity = 27/58 (46.55%), Postives = 41/58 (70.69%), Query Frame = 3
Query: 54 YGYLDKGEFTSELFKIEIKNLHPKVGFSQLKQRLKSIGLNPHKVKI--TNECSFVTFK 221
Y Y+ + FTSE++K+EI+NL +GF+ LK+ L G+NPHK+K+ + +FVTFK
Sbjct: 63 YRYIKEDLFTSEIYKVEIQNLPKYIGFNDLKKFLNKHGINPHKIKLLGRQKFAFVTFK 120
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Zebrafish
Match: trmt2b (tRNA methyltransferase 2 homolog B [Source:NCBI gene;Acc:606657]) HSP 1 Score: 249.98 bits (637), Expect = 1.018e-75 Identity = 160/478 (33.47%), Postives = 235/478 (49.16%), Query Frame = 1
Query: 352 SDCKKIKLENDDISNEEKLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKENILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIGDTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGT---DQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTRRKNNSDPLDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEA-AYNNFIDFARPIS--KRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTR 1767
S+ KK KL +S EE+L D V WR Y++QL K K IL + +++++ ++ F +N PLLPI+ SPV D YRNK F++ ++ GF G + G L IP + V D + LS +P D GH R IT+RT S + I+ HPQ L+ E EYF G G C + SL + + + ++G +YE+++ FRIS ++FFQVN AAE LY T+ +L S TLLDVCCGTG IG+ ++ +++VIG+E+ ++VEDA +N LN + F AGKAE ++ +L+ + A+V+P RAGLH V++ALR I++++++SC P+ A NF + + +R G F P +AVPVDLFPHTPH ELV++ R
Sbjct: 48 SNAKKPKLPTTKLSWEERLSDTVTPLWRMTYEDQLQWKYEHQKKILLKMMKELSQD-----PTRAFSDHLN-----FPLLPIVASPVRDGYRNKSTFSVNKGIDGNPKTLGFYIGTGKAGNIVCVHADHLLSIPSKHKMVARCYEDFI----------RLSPLRPCILFDDGGHWREITIRTNSTG-------------HTMAIVYFHPQSLTPEETDIHKAALVEYFTQGPGAICQLDSLYFQETTMTRCSHEQSQYQLLYGQTHIYEEVLGFKFRISPDSFFQVNREAAEALYKTVAEL-------SQPCVGGTLLDVCCGTGAIGISLSPQMERVIGIELIEQAVEDAKFNAALNRVCNCEFLAGKAEVVLPDLMGSLSSDG-----GLTAVVNPSRAGLHYRVVRALRNHSAIRRLVYISCKPDGEAMRNFRELCCSVGLVRRITGEAFKPVVAVPVDLFPHTPHCELVLVFER 480
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Zebrafish
Match: trmt2b (tRNA methyltransferase 2 homolog B [Source:NCBI gene;Acc:606657]) HSP 1 Score: 249.21 bits (635), Expect = 1.420e-75 Identity = 160/478 (33.47%), Postives = 235/478 (49.16%), Query Frame = 1
Query: 352 SDCKKIKLENDDISNEEKLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKENILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIGDTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGT---DQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTRRKNNSDPLDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEA-AYNNFIDFARPIS--KRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTR 1767
S+ KK KL +S EE+L D V WR Y++QL K K IL + +++++ ++ F +N PLLPI+ SPV D YRNK F++ ++ GF G + G L IP + V D + LS +P D GH R IT+RT S + I+ HPQ L+ E EYF G G C + SL + + + ++G +YE+++ FRIS ++FFQVN AAE LY T+ +L S TLLDVCCGTG IG+ ++ +++VIG+E+ ++VEDA +N LN + F AGKAE ++ +L+ + A+V+P RAGLH V++ALR I++++++SC P+ A NF + + +R G F P +AVPVDLFPHTPH ELV++ R
Sbjct: 41 SNAKKPKLPTTKLSWEERLSDTVTPLWRMTYEDQLQWKYEHQKKILLKMMKELSQD-----PTRAFSDHLN-----FPLLPIVASPVRDGYRNKSTFSVNKGIDGNPKTLGFYIGTGKAGNIVCVHADHLLSIPSKHKMVARCYEDFI----------RLSPLRPCILFDDGGHWREITIRTNSTG-------------HTMAIVYFHPQSLTPEETDIHKAALVEYFTQGPGAICQLDSLYFQETTMTRCSHEQSQYQLLYGQTHIYEEVLGFKFRISPDSFFQVNREAAEALYKTVAEL-------SQPCVGGTLLDVCCGTGAIGISLSPQMERVIGIELIEQAVEDAKFNAALNRVCNCEFLAGKAEVVLPDLMGSLSSDG-----GLTAVVNPSRAGLHYRVVRALRNHSAIRRLVYISCKPDGEAMRNFRELCCSVGLVRRITGEAFKPVVAVPVDLFPHTPHCELVLVFER 473
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Xenopus
Match: hspa12b (heat shock protein family A (Hsp70) member 12B [Source:Xenbase;Acc:XB-GENE-1003961]) HSP 1 Score: 328.561 bits (841), Expect = 6.199e-104 Identity = 200/528 (37.88%), Postives = 278/528 (52.65%), Query Frame = 1
Query: 220 KRDEAISKVTGHNWKGNVLLAKVAKGRADPFLQKRKEMENVTEISDCKKIKLENDDISNEEKLK----DHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKENILIK----ESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIGDTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTRRKNNSDP-LDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYNNFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTRLAP 1776
+RD+A+ + G WK L ++AK RADP +QKRK+ E E + K+ ND + EE L D V W+ PY+ QL KE + +L+ + +I N + E K Y N + CPL +IPSP+ YRNK EF IG+ + G KY+GG A+ + P + + V ++ + I TP V+ P T GH + +T+RT ++ I+ +PQ L + +L + + YF G GK VTSL P L++ + I G ++YE L+ L FRIS +AFFQVNT AAE LYS I + ++ T+LDVCCGTGTIG+ +A V KV+G+E+C E++ DA N +LNN+ F GKAED+ L+ + + +AIVDPPRAGLH VI A+R+ E +K++I+VSCNP+AA NNFID R S R G PF P A+ VDLFP TPH ELV+L R P
Sbjct: 127 ERDKAMKVIHGVKWKNRTLSVRLAKPRADPIMQKRKQEEGDGEQPESKRQAAHND--AEEEPLSKTISDVVTPLWQVPYEEQLKIKEEECCKVLKQLTKEIGVNNKPLAPWLFEQKQKY-----NRLCCPLERVIPSPIQTEYRNKSEFLIGYGVNQEDKTVGCRLSKYKGGTCAVVE----PFDTINIPPVTKNVVRAFQEYIRS--TP-YDVYSPETYAGHWKQLTVRTSRKG-------------QVMAIVYFNPQKLPKEDLSELKTSLSMYFTEGSGKESGVTSLYFVEEGQRKSPHLEDLPVEHIAGDQYIYEDLLGLTFRISPHAFFQVNTPAAEVLYSAIADW-------AQLDQNTTVLDVCCGTGTIGISLAKKVKKVVGIELCQEAIADAKANAQLNNLDNVEFRCGKAEDIFPTLIYSF------TFPSPLAIVDPPRAGLHSKVIIAIRKAEHLKQLIYVSCNPKAAMNNFIDLCRAPSNRVKGRPFRPIKALAVDLFPQTPHFELVILFERTEP 614 HSP 2 Score: 55.0694 bits (131), Expect = 2.700e-7 Identity = 29/59 (49.15%), Postives = 41/59 (69.49%), Query Frame = 3
Query: 54 YGYLDKGEFTSELFKIEIKNLHPKVGFSQLKQRLKSIGLNPHKVKITNEC--SFVTFKT 224
Y Y+ FTSE+FK+EI+NL + F+ LK+ L GLNPHK+K+ N+ +FVTFK+
Sbjct: 66 YRYIKDDLFTSEIFKVEIQNLPKYICFNDLKKFLGKYGLNPHKIKLMNKQTFAFVTFKS 124
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Xenopus
Match: hspa12b (heat shock protein family A (Hsp70) member 12B [Source:Xenbase;Acc:XB-GENE-1003961]) HSP 1 Score: 327.405 bits (838), Expect = 1.525e-103 Identity = 200/528 (37.88%), Postives = 278/528 (52.65%), Query Frame = 1
Query: 220 KRDEAISKVTGHNWKGNVLLAKVAKGRADPFLQKRKEMENVTEISDCKKIKLENDDISNEEKLK----DHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKENILIK----ESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIGDTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTRRKNNSDP-LDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYNNFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTRLAP 1776
+RD+A+ + G WK L ++AK RADP +QKRK+ E E + K+ ND + EE L D V W+ PY+ QL KE + +L+ + +I N + E K Y N + CPL +IPSP+ YRNK EF IG+ + G KY+GG A+ + P + + V ++ + I TP V+ P T GH + +T+RT ++ I+ +PQ L + +L + + YF G GK VTSL P L++ + I G ++YE L+ L FRIS +AFFQVNT AAE LYS I + ++ T+LDVCCGTGTIG+ +A V KV+G+E+C E++ DA N +LNN+ F GKAED+ L+ + + +AIVDPPRAGLH VI A+R+ E +K++I+VSCNP+AA NNFID R S R G PF P A+ VDLFP TPH ELV+L R P
Sbjct: 89 ERDKAMKVIHGVKWKNRTLSVRLAKPRADPIMQKRKQEEGDGEQPESKRQAAHND--AEEEPLSKTISDVVTPLWQVPYEEQLKIKEEECCKVLKQLTKEIGVNNKPLAPWLFEQKQKY-----NRLCCPLERVIPSPIQTEYRNKSEFLIGYGVNQEDKTVGCRLSKYKGGTCAVVE----PFDTINIPPVTKNVVRAFQEYIRS--TP-YDVYSPETYAGHWKQLTVRTSRKG-------------QVMAIVYFNPQKLPKEDLSELKTSLSMYFTEGSGKESGVTSLYFVEEGQRKSPHLEDLPVEHIAGDQYIYEDLLGLTFRISPHAFFQVNTPAAEVLYSAIADW-------AQLDQNTTVLDVCCGTGTIGISLAKKVKKVVGIELCQEAIADAKANAQLNNLDNVEFRCGKAEDIFPTLIYSF------TFPSPLAIVDPPRAGLHSKVIIAIRKAEHLKQLIYVSCNPKAAMNNFIDLCRAPSNRVKGRPFRPIKALAVDLFPQTPHFELVILFERTEP 576 HSP 2 Score: 55.0694 bits (131), Expect = 2.671e-7 Identity = 29/59 (49.15%), Postives = 41/59 (69.49%), Query Frame = 3
Query: 54 YGYLDKGEFTSELFKIEIKNLHPKVGFSQLKQRLKSIGLNPHKVKITNEC--SFVTFKT 224
Y Y+ FTSE+FK+EI+NL + F+ LK+ L GLNPHK+K+ N+ +FVTFK+
Sbjct: 28 YRYIKDDLFTSEIFKVEIQNLPKYICFNDLKKFLGKYGLNPHKIKLMNKQTFAFVTFKS 86
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Xenopus
Match: hspa12b (heat shock protein family A (Hsp70) member 12B [Source:Xenbase;Acc:XB-GENE-1003961]) HSP 1 Score: 318.931 bits (816), Expect = 1.463e-100 Identity = 196/524 (37.40%), Postives = 271/524 (51.72%), Query Frame = 1
Query: 220 KRDEAISKVTGHNWKGNVLLAKVAKGRADPFLQKRKEMENVTEISDCKKIKLENDDISNEEKLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKEN----ILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIGDTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTRRKNNSDP-LDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYNNFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTRLAP 1776
+RD+A+ + G WK L ++AK RADP +QKRK+ E E K I D V W+ PY+ QL KE + +L+ + +I N + E K Y N + CPL +IPSP+ YRNK EF IG+ + G KY+GG A+ + P + + V ++ + I TP V+ P T GH + +T+RT ++ I+ +PQ L + +L + + YF G GK VTSL P L++ + I G ++YE L+ L FRIS +AFFQVNT AAE LYS I + ++ T+LDVCCGTGTIG+ +A V KV+G+E+C E++ DA N +LNN+ F GKAED+ L+ + + +AIVDPPRAGLH VI A+R+ E +K++I+VSCNP+AA NNFID R S R G PF P A+ VDLFP TPH ELV+L R P
Sbjct: 103 ERDKAMKVIHGVKWKNRTLSVRLAKPRADPIMQKRKQEEGDEEEPLSKTIS-------------DVVTPLWQVPYEEQLKIKEEECCKVLKQLTKEIGVNNKPLAPWLFEQKQKY-----NRLCCPLERVIPSPIQTEYRNKSEFLIGYGVNQEDKTVGCRLSKYKGGTCAVVE----PFDTINIPPVTKNVVRAFQEYIRS--TP-YDVYSPETYAGHWKQLTVRTSRKG-------------QVMAIVYFNPQKLPKEDLSELKTSLSMYFTEGSGKESGVTSLYFVEEGQRKSPHLEDLPVEHIAGDQYIYEDLLGLTFRISPHAFFQVNTPAAEVLYSAIADW-------AQLDQNTTVLDVCCGTGTIGISLAKKVKKVVGIELCQEAIADAKANAQLNNLDNVEFRCGKAEDIFPTLIYSF------TFPSPLAIVDPPRAGLHSKVIIAIRKAEHLKQLIYVSCNPKAAMNNFIDLCRAPSNRVKGRPFRPIKALAVDLFPQTPHFELVILFERTEP 575 HSP 2 Score: 55.0694 bits (131), Expect = 2.984e-7 Identity = 29/59 (49.15%), Postives = 41/59 (69.49%), Query Frame = 3
Query: 54 YGYLDKGEFTSELFKIEIKNLHPKVGFSQLKQRLKSIGLNPHKVKITNEC--SFVTFKT 224
Y Y+ FTSE+FK+EI+NL + F+ LK+ L GLNPHK+K+ N+ +FVTFK+
Sbjct: 42 YRYIKDDLFTSEIFKVEIQNLPKYICFNDLKKFLGKYGLNPHKIKLMNKQTFAFVTFKS 100
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Xenopus
Match: hspa12b (heat shock protein family A (Hsp70) member 12B [Source:Xenbase;Acc:XB-GENE-1003961]) HSP 1 Score: 314.309 bits (804), Expect = 3.890e-99 Identity = 192/524 (36.64%), Postives = 268/524 (51.15%), Query Frame = 1
Query: 220 KRDEAISKVTGHNWKGNVLLAKVAKGRADPFLQKRKEMENVTEISDCKKIKLENDDISNEEKLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKENILIK----ESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIGDTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTRRKNNSDP-LDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYNNFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTRLAP 1776
+RD+A+ + G WK L ++AK RADP +QKR N + + D V W+ PY+ QL KE + +L+ + +I N + E K Y N + CPL +IPSP+ YRNK EF IG+ + G KY+GG A+ + P + + V ++ + I TP V+ P T GH + +T+RT ++ I+ +PQ L + +L + + YF G GK VTSL P L++ + I G ++YE L+ L FRIS +AFFQVNT AAE LYS I + ++ T+LDVCCGTGTIG+ +A V KV+G+E+C E++ DA N +LNN+ F GKAED+ L+ + + +AIVDPPRAGLH VI A+R+ E +K++I+VSCNP+AA NNFID R S R G PF P A+ VDLFP TPH ELV+L R P
Sbjct: 91 ERDKAMKVIHGVKWKNRTLSVRLAKPRADPIMQKR-----------------------NNKTISDVVTPLWQVPYEEQLKIKEEECCKVLKQLTKEIGVNNKPLAPWLFEQKQKY-----NRLCCPLERVIPSPIQTEYRNKSEFLIGYGVNQEDKTVGCRLSKYKGGTCAVVE----PFDTINIPPVTKNVVRAFQEYIRS--TP-YDVYSPETYAGHWKQLTVRTSRKG-------------QVMAIVYFNPQKLPKEDLSELKTSLSMYFTEGSGKESGVTSLYFVEEGQRKSPHLEDLPVEHIAGDQYIYEDLLGLTFRISPHAFFQVNTPAAEVLYSAIADW-------AQLDQNTTVLDVCCGTGTIGISLAKKVKKVVGIELCQEAIADAKANAQLNNLDNVEFRCGKAEDIFPTLIYSF------TFPSPLAIVDPPRAGLHSKVIIAIRKAEHLKQLIYVSCNPKAAMNNFIDLCRAPSNRVKGRPFRPIKALAVDLFPQTPHFELVILFERTEP 553 HSP 2 Score: 55.0694 bits (131), Expect = 2.345e-7 Identity = 29/59 (49.15%), Postives = 41/59 (69.49%), Query Frame = 3
Query: 54 YGYLDKGEFTSELFKIEIKNLHPKVGFSQLKQRLKSIGLNPHKVKITNEC--SFVTFKT 224
Y Y+ FTSE+FK+EI+NL + F+ LK+ L GLNPHK+K+ N+ +FVTFK+
Sbjct: 30 YRYIKDDLFTSEIFKVEIQNLPKYICFNDLKKFLGKYGLNPHKIKLMNKQTFAFVTFKS 88
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Xenopus
Match: hspa12b (heat shock protein family A (Hsp70) member 12B [Source:Xenbase;Acc:XB-GENE-1003961]) HSP 1 Score: 314.309 bits (804), Expect = 4.103e-99 Identity = 192/524 (36.64%), Postives = 268/524 (51.15%), Query Frame = 1
Query: 220 KRDEAISKVTGHNWKGNVLLAKVAKGRADPFLQKRKEMENVTEISDCKKIKLENDDISNEEKLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKEN----ILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIGDTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTRRKNNSDP-LDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYNNFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTRLAP 1776
+RD+A+ + G WK L ++AK RADP +QKR N + + D V W+ PY+ QL KE + +L+ + +I N + E K Y N + CPL +IPSP+ YRNK EF IG+ + G KY+GG A+ + P + + V ++ + I TP V+ P T GH + +T+RT ++ I+ +PQ L + +L + + YF G GK VTSL P L++ + I G ++YE L+ L FRIS +AFFQVNT AAE LYS I + ++ T+LDVCCGTGTIG+ +A V KV+G+E+C E++ DA N +LNN+ F GKAED+ L+ + + +AIVDPPRAGLH VI A+R+ E +K++I+VSCNP+AA NNFID R S R G PF P A+ VDLFP TPH ELV+L R P
Sbjct: 91 ERDKAMKVIHGVKWKNRTLSVRLAKPRADPIMQKR-----------------------NNKTISDVVTPLWQVPYEEQLKIKEEECCKVLKQLTKEIGVNNKPLAPWLFEQKQKY-----NRLCCPLERVIPSPIQTEYRNKSEFLIGYGVNQEDKTVGCRLSKYKGGTCAVVE----PFDTINIPPVTKNVVRAFQEYIRS--TP-YDVYSPETYAGHWKQLTVRTSRKG-------------QVMAIVYFNPQKLPKEDLSELKTSLSMYFTEGSGKESGVTSLYFVEEGQRKSPHLEDLPVEHIAGDQYIYEDLLGLTFRISPHAFFQVNTPAAEVLYSAIADW-------AQLDQNTTVLDVCCGTGTIGISLAKKVKKVVGIELCQEAIADAKANAQLNNLDNVEFRCGKAEDIFPTLIYSF------TFPSPLAIVDPPRAGLHSKVIIAIRKAEHLKQLIYVSCNPKAAMNNFIDLCRAPSNRVKGRPFRPIKALAVDLFPQTPHFELVILFERTEP 553 HSP 2 Score: 55.4546 bits (132), Expect = 2.265e-7 Identity = 29/59 (49.15%), Postives = 41/59 (69.49%), Query Frame = 3
Query: 54 YGYLDKGEFTSELFKIEIKNLHPKVGFSQLKQRLKSIGLNPHKVKITNEC--SFVTFKT 224
Y Y+ FTSE+FK+EI+NL + F+ LK+ L GLNPHK+K+ N+ +FVTFK+
Sbjct: 30 YRYIKDDLFTSEIFKVEIQNLPKYICFNDLKKFLGKYGLNPHKIKLMNKQTFAFVTFKS 88
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Mouse
Match: Trmt2a (TRM2 tRNA methyltransferase 2A [Source:MGI Symbol;Acc:MGI:96270]) HSP 1 Score: 309.686 bits (792), Expect = 5.235e-97 Identity = 187/521 (35.89%), Postives = 260/521 (49.90%), Query Frame = 1
Query: 220 KRDEAISKVTGHNWKGNVLLAKVAKGRADPFLQKRKEMENVTEISDCKKIKLENDDISNEEKLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKENILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIG---DTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTRRKNNSDP-LDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYNNFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTRL 1770
+RD+A+ + G WKG L ++A+ +ADP +KR++ E D + ++ D V W PY QL K + + +LQ + +I N + Q N CPL + PSP YRNKCEF +G ++ G GKY+GG A+ DT +P Q+ K P S + P T GH + +T+RT S + I HPQ LS E+ + +F+ G GK VTSL P + + + G + E L+ L FRIS +AFFQVNT AAE LY+ I++ +D S T+LDVCCGTGTIGL +A V +V+G+E+C E+VEDA N N ++ FH G+AED++ L+S Q VA++DPPRAGLH VI A+R+ E IK++++VSCNP AA NF+D R S R G PF P AV VDLFP TPH E+++L R+
Sbjct: 111 ERDKALRVLHGALWKGCPLSVRLARPKADPMARKRRQ---------------EGDSEPSVTQIADVVTPLWTVPYTEQLEQKRLECERVLQKLAKEIGNTNRALLPWLLLQRQ-QHNKACCPLEGVKPSPQQTEYRNKCEFLVGVGVDGKDNTVGCRLGKYKGGTCAVAAPFDTVHIPEATKQVVKAFQEFIRSTP----------YSAYDPETYTGHWKQLTVRTSSRG-------------QAMAIAYFHPQKLSSEEVAGLKASLVCHFMEGPGKASGVTSLYFVEEGQRKTPSQEGLPLEHMAGDQCIQEDLLGLTFRISPHAFFQVNTPAAEVLYTVIQEWA---QLDGGS----TVLDVCCGTGTIGLALAPKVKRVVGIELCQEAVEDARMNALTNELSNVEFHCGRAEDLVPGLVSRLSSHQ------LVAVLDPPRAGLHSKVILAIRKAENIKRLLYVSCNPRAAMGNFVDLCRAPSNRVKGTPFHPVKAVAVDLFPQTPHCEMLILFERM 579
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Mouse
Match: Trmt2a (TRM2 tRNA methyltransferase 2A [Source:MGI Symbol;Acc:MGI:96270]) HSP 1 Score: 309.686 bits (792), Expect = 5.235e-97 Identity = 187/521 (35.89%), Postives = 260/521 (49.90%), Query Frame = 1
Query: 220 KRDEAISKVTGHNWKGNVLLAKVAKGRADPFLQKRKEMENVTEISDCKKIKLENDDISNEEKLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKENILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIG---DTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTRRKNNSDP-LDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYNNFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTRL 1770
+RD+A+ + G WKG L ++A+ +ADP +KR++ E D + ++ D V W PY QL K + + +LQ + +I N + Q N CPL + PSP YRNKCEF +G ++ G GKY+GG A+ DT +P Q+ K P S + P T GH + +T+RT S + I HPQ LS E+ + +F+ G GK VTSL P + + + G + E L+ L FRIS +AFFQVNT AAE LY+ I++ +D S T+LDVCCGTGTIGL +A V +V+G+E+C E+VEDA N N ++ FH G+AED++ L+S Q VA++DPPRAGLH VI A+R+ E IK++++VSCNP AA NF+D R S R G PF P AV VDLFP TPH E+++L R+
Sbjct: 111 ERDKALRVLHGALWKGCPLSVRLARPKADPMARKRRQ---------------EGDSEPSVTQIADVVTPLWTVPYTEQLEQKRLECERVLQKLAKEIGNTNRALLPWLLLQRQ-QHNKACCPLEGVKPSPQQTEYRNKCEFLVGVGVDGKDNTVGCRLGKYKGGTCAVAAPFDTVHIPEATKQVVKAFQEFIRSTP----------YSAYDPETYTGHWKQLTVRTSSRG-------------QAMAIAYFHPQKLSSEEVAGLKASLVCHFMEGPGKASGVTSLYFVEEGQRKTPSQEGLPLEHMAGDQCIQEDLLGLTFRISPHAFFQVNTPAAEVLYTVIQEWA---QLDGGS----TVLDVCCGTGTIGLALAPKVKRVVGIELCQEAVEDARMNALTNELSNVEFHCGRAEDLVPGLVSRLSSHQ------LVAVLDPPRAGLHSKVILAIRKAENIKRLLYVSCNPRAAMGNFVDLCRAPSNRVKGTPFHPVKAVAVDLFPQTPHCEMLILFERM 579
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Mouse
Match: Trmt2a (TRM2 tRNA methyltransferase 2A [Source:MGI Symbol;Acc:MGI:96270]) HSP 1 Score: 309.301 bits (791), Expect = 7.351e-97 Identity = 187/521 (35.89%), Postives = 260/521 (49.90%), Query Frame = 1
Query: 220 KRDEAISKVTGHNWKGNVLLAKVAKGRADPFLQKRKEMENVTEISDCKKIKLENDDISNEEKLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKENILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIG---DTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTRRKNNSDP-LDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYNNFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTRL 1770
+RD+A+ + G WKG L ++A+ +ADP +KR++ E D + ++ D V W PY QL K + + +LQ + +I N + Q N CPL + PSP YRNKCEF +G ++ G GKY+GG A+ DT +P Q+ K P S + P T GH + +T+RT S + I HPQ LS E+ + +F+ G GK VTSL P + + + G + E L+ L FRIS +AFFQVNT AAE LY+ I++ +D S T+LDVCCGTGTIGL +A V +V+G+E+C E+VEDA N N ++ FH G+AED++ L+S Q VA++DPPRAGLH VI A+R+ E IK++++VSCNP AA NF+D R S R G PF P AV VDLFP TPH E+++L R+
Sbjct: 111 ERDKALRVLHGALWKGCPLSVRLARPKADPMARKRRQ---------------EGDSEPSVTQIADVVTPLWTVPYTEQLEQKRLECERVLQKLAKEIGNTNRALLPWLLLQRQ-QHNKACCPLEGVKPSPQQTEYRNKCEFLVGVGVDGKDNTVGCRLGKYKGGTCAVAAPFDTVHIPEATKQVVKAFQEFIRSTP----------YSAYDPETYTGHWKQLTVRTSSRG-------------QAMAIAYFHPQKLSSEEVAGLKASLVCHFMEGPGKASGVTSLYFVEEGQRKTPSQEGLPLEHMAGDQCIQEDLLGLTFRISPHAFFQVNTPAAEVLYTVIQEWA---QLDGGS----TVLDVCCGTGTIGLALAPKVKRVVGIELCQEAVEDARMNALTNELSNVEFHCGRAEDLVPGLVSRLSSHQ------LVAVLDPPRAGLHSKVILAIRKAENIKRLLYVSCNPRAAMGNFVDLCRAPSNRVKGTPFHPVKAVAVDLFPQTPHCEMLILFERM 579
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Mouse
Match: Trmt2a (TRM2 tRNA methyltransferase 2A [Source:MGI Symbol;Acc:MGI:96270]) HSP 1 Score: 309.301 bits (791), Expect = 7.351e-97 Identity = 187/521 (35.89%), Postives = 260/521 (49.90%), Query Frame = 1
Query: 220 KRDEAISKVTGHNWKGNVLLAKVAKGRADPFLQKRKEMENVTEISDCKKIKLENDDISNEEKLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKENILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIG---DTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTRRKNNSDP-LDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYNNFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTRL 1770
+RD+A+ + G WKG L ++A+ +ADP +KR++ E D + ++ D V W PY QL K + + +LQ + +I N + Q N CPL + PSP YRNKCEF +G ++ G GKY+GG A+ DT +P Q+ K P S + P T GH + +T+RT S + I HPQ LS E+ + +F+ G GK VTSL P + + + G + E L+ L FRIS +AFFQVNT AAE LY+ I++ +D S T+LDVCCGTGTIGL +A V +V+G+E+C E+VEDA N N ++ FH G+AED++ L+S Q VA++DPPRAGLH VI A+R+ E IK++++VSCNP AA NF+D R S R G PF P AV VDLFP TPH E+++L R+
Sbjct: 111 ERDKALRVLHGALWKGCPLSVRLARPKADPMARKRRQ---------------EGDSEPSVTQIADVVTPLWTVPYTEQLEQKRLECERVLQKLAKEIGNTNRALLPWLLLQRQ-QHNKACCPLEGVKPSPQQTEYRNKCEFLVGVGVDGKDNTVGCRLGKYKGGTCAVAAPFDTVHIPEATKQVVKAFQEFIRSTP----------YSAYDPETYTGHWKQLTVRTSSRG-------------QAMAIAYFHPQKLSSEEVAGLKASLVCHFMEGPGKASGVTSLYFVEEGQRKTPSQEGLPLEHMAGDQCIQEDLLGLTFRISPHAFFQVNTPAAEVLYTVIQEWA---QLDGGS----TVLDVCCGTGTIGLALAPKVKRVVGIELCQEAVEDARMNALTNELSNVEFHCGRAEDLVPGLVSRLSSHQ------LVAVLDPPRAGLHSKVILAIRKAENIKRLLYVSCNPRAAMGNFVDLCRAPSNRVKGTPFHPVKAVAVDLFPQTPHCEMLILFERM 579
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Mouse
Match: Trmt2a (TRM2 tRNA methyltransferase 2A [Source:MGI Symbol;Acc:MGI:96270]) HSP 1 Score: 251.521 bits (641), Expect = 2.380e-75 Identity = 170/521 (32.63%), Postives = 236/521 (45.30%), Query Frame = 1
Query: 220 KRDEAISKVTGHNWKGNVLLAKVAKGRADPFLQKRKEMENVTEISDCKKIKLENDDISNEEKLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKENILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIG---DTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTRRKNNSDP-LDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYNNFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTRL 1770
+RD+A+ + G WKG L ++A+ +ADP +KR++ E D + ++ D V W PY QL K + + +LQ + +I N + Q N CPL + PSP YRNKCEF +G ++ G GKY+GG A+ DT +P Q+ K P S + P T GH + +T+RT S + I HPQ LS E+ + +F+ G GK VTSL P + + + G + E L+ L FRIS +AFFQVNT AAE LY+ I++ +D S T+LDVCCGTGTIGL +A V +V+G+E+C E+VEDA N L N +K VI A+R+ E IK++++VSCNP AA NF+D R S R G PF P AV VDLFP TPH E+++L R+
Sbjct: 111 ERDKALRVLHGALWKGCPLSVRLARPKADPMARKRRQ---------------EGDSEPSVTQIADVVTPLWTVPYTEQLEQKRLECERVLQKLAKEIGNTNRALLPWLLLQRQ-QHNKACCPLEGVKPSPQQTEYRNKCEFLVGVGVDGKDNTVGCRLGKYKGGTCAVAAPFDTVHIPEATKQVVKAFQEFIRSTP----------YSAYDPETYTGHWKQLTVRTSSRG-------------QAMAIAYFHPQKLSSEEVAGLKASLVCHFMEGPGKASGVTSLYFVEEGQRKTPSQEGLPLEHMAGDQCIQEDLLGLTFRISPHAFFQVNTPAAEVLYTVIQEWA---QLDGGS----TVLDVCCGTGTIGLALAPKVKRVVGIELCQEAVEDARMNA-LTNDSK--------------------------------------------VILAIRKAENIKRLLYVSCNPRAAMGNFVDLCRAPSNRVKGTPFHPVKAVAVDLFPQTPHCEMLILFERM 540
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. UniProt/SwissProt
Match: sp|Q8IZ69|TRM2A_HUMAN (tRNA (uracil-5-)-methyltransferase homolog A OS=Homo sapiens OX=9606 GN=TRMT2A PE=1 SV=2) HSP 1 Score: 306.605 bits (784), Expect = 8.180e-95 Identity = 183/521 (35.12%), Postives = 262/521 (50.29%), Query Frame = 1
Query: 220 KRDEAISKVTGHNWKGNVLLAKVAKGRADPFLQKRKEMENVTEISDCKKIKLENDDISNEEKLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKENILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIG---DTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTRRKNNSDP-LDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYNNFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTRL 1770
+RD+A+ + G WKG L ++A+ +ADP ++R++ E + ++ D V W PY QL K+ + + +LQ + +I N + + N CPL + PSP YRNKCEF +G ++ G GKY+GG A+ DT +P Q+ K P S + P T GH + +T+RT + + I HPQ LS EL + A++F G G+ VT L P + + + G ++E L+ L FRIS +AFFQVNT AAE LY+ I+ +D+ S +LDVCCGTGTIGL +A V +VIGVE+CPE+VEDA N + N ++ FH G+AED++ L+S + VAI+DPPRAGLH VI A+RR + ++++++VSCNP AA NF+D R S R G PF P AV VDLFP TPH E+++L R+
Sbjct: 121 ERDKALRVLHGALWKGRPLSVRLARPKADPMARRRRQ---------------EGESEPPVTRVADVVTPLWTVPYAEQLERKQLECEQVLQKLAKEIGSTNRALLPWL-LEQRHKHNKACCPLEGVRPSPQQTEYRNKCEFLVGVGVDGEDNTVGCRLGKYKGGTCAVAAPFDTVHIPEATKQVVKAFQEFIRSTP----------YSAYDPETYTGHWKQLTVRTSRRH-------------QAMAIAYFHPQKLSPEELAELKTSLAQHFTAGPGRASGVTCLYFVEEGQRKTPSQEGLPLEHVAGDRCIHEDLLGLTFRISPHAFFQVNTPAAEVLYTVIQDWA---QLDAGS----MVLDVCCGTGTIGLALARKVKRVIGVELCPEAVEDARVNAQDNELSNVEFHCGRAEDLVPTLVSRLAS------QHLVAILDPPRAGLHSKVILAIRRAKNLRRLLYVSCNPRAAMGNFVDLCRAPSNRVKGIPFRPVKAVAVDLFPQTPHCEMLILFERV 589
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. UniProt/SwissProt
Match: sp|Q96GJ1|TRM2_HUMAN (tRNA (uracil(54)-C(5))-methyltransferase homolog OS=Homo sapiens OX=9606 GN=TRMT2B PE=1 SV=1) HSP 1 Score: 259.61 bits (662), Expect = 3.004e-78 Identity = 167/464 (35.99%), Postives = 234/464 (50.43%), Query Frame = 1
Query: 385 DISNEEKLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKENILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIGDTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTRRKNNSDPLDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEA-AYNNFIDFARP--ISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTR 1767
D S +E+L D V WR Y+ QL K K ILQ + I N + + + + C L PIIPSPV + YRNK F++ + GF G +R G ++ L IP + S+V + L Q P L VF G G+ R +T+RT S + II HPQ LS+ EL + E+F+ G G C +TSL + + +FG +++E+L++L RIS +AFFQ+NT AE LY T+ +L + LLD+CCGTG IGL +A +V+G+E+ ++VEDA + N IT + FH G+AE ++ LL + E Q VA+V+P RAGLH VIQA+R I ++FVSC + N I+ P +K+ G PF+ + AVPVDLFPHTPH ELV+L TR
Sbjct: 77 DGSWQERLADVVTPLWRLSYEEQLKVKFAAQKKILQRLESYIQMLNGVSVTT----AVPKSERLSCLLHPIIPSPVINGYRNKSTFSVNRGPDGNPKTVGFYLGTWRDGNVVCVQSNHLKNIPEKHSQVAQYYEVFLRQ---SPLEPCL-VFHEG---GYWRELTVRTNSQG-------------HTMAIITFHPQKLSQEELHVQKEIVKEFFIRGPGAACGLTSLYFQESTMTRCSHQQSPYQLLFGEPYIFEELLSLKIRISPDAFFQINTAGAEMLYRTVGEL-------TGVNSDTILLDICCGTGVIGLSLAQHTSRVLGIELLEQAVEDARWTAAFNGITNSEFHTGQAEKILPGLLKSKEDGQ-----SIVAVVNPARAGLHYKVIQAIRNFRAIHTLVFVSCKLHGESTRNVIELCCPPDPAKKLLGEPFVLQQAVPVDLFPHTPHCELVLLFTR 504
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. UniProt/SwissProt
Match: sp|Q5RFM7|TRM2_PONAB (tRNA (uracil(54)-C(5))-methyltransferase homolog OS=Pongo abelii OX=9601 GN=TRMT2B PE=2 SV=1) HSP 1 Score: 255.758 bits (652), Expect = 5.255e-77 Identity = 165/464 (35.56%), Postives = 234/464 (50.43%), Query Frame = 1
Query: 385 DISNEEKLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKENILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIGDTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTRRKNNSDPLDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEA-AYNNFIDFARP--ISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTR 1767
D S +E+L D V WR Y+ QL K K ILQ + I N + + S+ + C L PIIPSPV + YRNK F++ + GF G +R G ++ L IP + S+V + L Q P L VF G G+ R +T+RT S + II HPQ LS+ E + E+F+ G G C++TSL + + +FG +++E+L++L RIS +AFFQ+NT AE LY T+ +L + LLD+CCGTG IGL ++ +V+G+E+ ++VEDA + N IT + FH G+AE ++ LL + E Q VA+V+P RAGLH VIQA+R I ++FVSC + N I+ P +K+ G PF+ + VPVDLFPHTPH ELV+L TR
Sbjct: 59 DGSWQERLADVVTPLWRLSYEEQLKVKFAAQKKILQRLESYIQMLNGVSVTTAVPKSE----RLSCLLHPIIPSPVINGYRNKSTFSVNRGPDGNPKTVGFYLGTWRDGNMVCVQSNHLKNIPEKHSQVAQYYEVFLRQ---SPLEPCL-VFHEG---GYWRELTVRTNSQG-------------HTMAIITFHPQNLSQEEFHVQKEIVKEFFIRGPGAACDLTSLYFQESTMTRCSHQQSPYQLLFGEPYIFEELLSLKIRISPDAFFQINTAGAEMLYRTVGEL-------TGVNSDTILLDICCGTGVIGLSLSQHTSRVLGIELVEQAVEDARWTAAFNGITNSEFHTGRAEKILPGLLKSKEDGQ-----SIVAVVNPARAGLHYKVIQAIRNFRAIHTLVFVSCKLHGESTRNVIELCCPPDPAKKLLGEPFVLQQVVPVDLFPHTPHCELVLLFTR 486
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. UniProt/SwissProt
Match: sp|A4QP75|TRM2B_DANRE (tRNA (uracil(54)-C(5))-methyltransferase homolog-B OS=Danio rerio OX=7955 GN=trmt2b PE=2 SV=2) HSP 1 Score: 249.98 bits (637), Expect = 7.684e-75 Identity = 160/478 (33.47%), Postives = 235/478 (49.16%), Query Frame = 1
Query: 352 SDCKKIKLENDDISNEEKLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKENILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIGDTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGT---DQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTRRKNNSDPLDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEA-AYNNFIDFARPIS--KRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTR 1767
S+ KK KL +S EE+L D V WR Y++QL K K IL + +++++ ++ F +N PLLPI+ SPV D YRNK F++ ++ GF G + G L IP + V D + LS +P D GH R IT+RT S + I+ HPQ L+ E EYF G G C + SL + + + ++G +YE+++ FRIS ++FFQVN AAE LY T+ +L S TLLDVCCGTG IG+ ++ +++VIG+E+ ++VEDA +N LN + F AGKAE ++ +L+ + A+V+P RAGLH V++ALR I++++++SC P+ A NF + + +R G F P +AVPVDLFPHTPH ELV++ R
Sbjct: 48 SNAKKPKLPTTKLSWEERLSDTVTPLWRMTYEDQLQWKYEHQKKILLKMMKELSQD-----PTRAFSDHLN-----FPLLPIVASPVRDGYRNKSTFSVNKGIDGNPKTLGFYIGTGKAGNIVCVHADHLLSIPSKHKMVARCYEDFI----------RLSPLRPCILFDDGGHWREITIRTNSTG-------------HTMAIVYFHPQSLTPEETDIHKAALVEYFTQGPGAICQLDSLYFQETTMTRCSHEQSQYQLLYGQTHIYEEVLGFKFRISPDSFFQVNREAAEALYKTVAEL-------SQPCVGGTLLDVCCGTGAIGISLSPQMERVIGIELIEQAVEDAKFNAALNRVCNCEFLAGKAEVVLPDLMGSLSSDG-----GLTAVVNPSRAGLHYRVVRALRNHSAIRRLVYISCKPDGEAMRNFRELCCSVGLVRRITGEAFKPVVAVPVDLFPHTPHCELVLVFER 480
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. UniProt/SwissProt
Match: sp|Q8BNV1|TRM2A_MOUSE (tRNA (uracil-5-)-methyltransferase homolog A OS=Mus musculus OX=10090 GN=Trmt2a PE=1 SV=1) HSP 1 Score: 251.521 bits (641), Expect = 1.665e-74 Identity = 170/521 (32.63%), Postives = 236/521 (45.30%), Query Frame = 1
Query: 220 KRDEAISKVTGHNWKGNVLLAKVAKGRADPFLQKRKEMENVTEISDCKKIKLENDDISNEEKLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKENILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIG---DTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTRRKNNSDP-LDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYNNFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTRL 1770
+RD+A+ + G WKG L ++A+ +ADP +KR++ E D + ++ D V W PY QL K + + +LQ + +I N + Q N CPL + PSP YRNKCEF +G ++ G GKY+GG A+ DT +P Q+ K P S + P T GH + +T+RT S + I HPQ LS E+ + +F+ G GK VTSL P + + + G + E L+ L FRIS +AFFQVNT AAE LY+ I++ +D S T+LDVCCGTGTIGL +A V +V+G+E+C E+VEDA N L N +K VI A+R+ E IK++++VSCNP AA NF+D R S R G PF P AV VDLFP TPH E+++L R+
Sbjct: 111 ERDKALRVLHGALWKGCPLSVRLARPKADPMARKRRQ---------------EGDSEPSVTQIADVVTPLWTVPYTEQLEQKRLECERVLQKLAKEIGNTNRALLPWLLLQRQ-QHNKACCPLEGVKPSPQQTEYRNKCEFLVGVGVDGKDNTVGCRLGKYKGGTCAVAAPFDTVHIPEATKQVVKAFQEFIRSTP----------YSAYDPETYTGHWKQLTVRTSSRG-------------QAMAIAYFHPQKLSSEEVAGLKASLVCHFMEGPGKASGVTSLYFVEEGQRKTPSQEGLPLEHMAGDQCIQEDLLGLTFRISPHAFFQVNTPAAEVLYTVIQEWA---QLDGGS----TVLDVCCGTGTIGLALAPKVKRVVGIELCQEAVEDARMNA-LTNDSK--------------------------------------------VILAIRKAENIKRLLYVSCNPRAAMGNFVDLCRAPSNRVKGTPFHPVKAVAVDLFPQTPHCEMLILFERM 540
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. TrEMBL
Match: A0A1S3J1N9 (tRNA (Uracil-5-)-methyltransferase homolog A OS=Lingula unguis OX=7574 GN=LOC106169006 PE=3 SV=1) HSP 1 Score: 363.229 bits (931), Expect = 3.229e-114 Identity = 209/529 (39.51%), Postives = 297/529 (56.14%), Query Frame = 1
Query: 223 RDEAISKVTGHNWKGNVLLAKVAKGRADPFLQKRKEMENV-TEISDCKKIK-----LENDDISNEEKLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKENILIK---ESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIGDTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTRRKNNSDPLDNTTT-KTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEA-AYNNFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTRLAP 1776
R + ++ + GH WKG VL AK A ADPF++++ E++ T+ KK + E D + +E+L + V W+ PY+ Q+ K+ ++ GIL+ I K N+ +K +++ Q+ C LL I PSP+ + YRNKCEFTIG + G + GF G+YR G + + S +P M + + D + G F P L V GH R +T RT + L+ II HPQ L+E EL EYF++G GK+C +TSL + D+ + + G + E+L+ L FRIS +AFFQVNTLA E LY + ++++ VT+LD+CCGTGTIGL +A V KVIG+EM P+++EDA +N +LN+I+ FH GKAED++ L+S+ + VA+VDPPRAGLH VI A+RRC + +VIFVSC+P++ +NF+DF RP SK+ G PFIP A+PVDLFPHT H ELVVL R P
Sbjct: 126 RQKGLNVLNGHQWKGQVLQAKKANPAADPFIKRQLEVQQQDTDEPQAKKARSGTDNQEEDKLPPQERLNNVVTPLWQVPYEEQIQTKQTEMDGILKKFAHVIEKGNLDLKSWIQNQRKTHQLK----CCTLLEIKPSPILESYRNKCEFTIG-KGSDGGKVVGFRMGRYRDGTCVVTEPSGCINLPQSMKDLAKALQDYIQTSKYGVFDPELQV-------GHYRQLTARTCQG-------------KDLMAIIIFHPQSLTEEELALEKQSLVEYFVSGGGKSCGITSLYFQASSSRVTGGDSEPAFELLHGEQHISEELLGLTFRISPDAFFQVNTLATEVLYQVVGDWCLENI-----NTDVTVLDICCGTGTIGLTLAKRVKKVIGIEMVPQAIEDAKHNAQLNDISNVEFHCGKAEDVLPELVSS-----RPCWGNAVAVVDPPRAGLHPKVIHAIRRCHALNRVIFVSCSPKSVVQSNFLDFVRPESKKMKGPPFIPVKAIPVDLFPHTSHCELVVLFERYNP 619 HSP 2 Score: 60.8474 bits (146), Expect = 6.986e-6 Identity = 31/61 (50.82%), Postives = 43/61 (70.49%), Query Frame = 3
Query: 48 NPYGYLDKGEFTSELFKIEIKNLHPKVGFSQLKQRLKSIGLNPHKVKITN---ECSFVTFK 221
+PY YL + FTSE+FKIE++NL K GFSQLK+RL ++ LNP K+K + +VTF+
Sbjct: 61 DPYEYLKREGFTSEIFKIEVRNLPRKFGFSQLKKRLINLELNPVKIKAASANGSVGYVTFR 121
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. TrEMBL
Match: A0A3B3SEU7 (tRNA methyltransferase 2 homolog A OS=Paramormyrops kingsleyae OX=1676925 PE=3 SV=1) HSP 1 Score: 362.844 bits (930), Expect = 4.392e-114 Identity = 207/519 (39.88%), Postives = 293/519 (56.45%), Query Frame = 1
Query: 220 KRDEAISKVTGHNWKGNVLLAKVAKGRADPFLQKRKEME-NVTEISDCKKIKLENDDISNEEKLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKENILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIGDTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTRRKNNSDP-LDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYNNFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTRL 1770
+RD A+ V G WKG VL ++AK +ADP L+KRK+ E +E DCK+ +L D +++ D V W PY+ QL K +++G+LQ + +I+ N + F + NN+ CPL I PSPV YRNKCEF IG GF GKY+GG A+ S +P + +V+ H D + T SV+ P T +GH + +T+RT + ++ ++ +PQ L+E EL + E+F G GK VTSL R + P L++ + + G +++E+L+ L FRIS ++FFQ+NT AAE LYST+ + +D S T+LDVCCGTGTIGL +A V KVIG+E+C E+VEDA N +N +T FH GKAED+ +LL+ ++ AIVDPPRAGLH VI A+RR E +K++++V+CNP+AA NNFID R S R G PF P + VDLFP T H E+++LL R+
Sbjct: 109 ERDRAMKAVHGMLWKGRVLSVRLAKPKADPILKKRKQEEAESSEQPDCKR-ELATDAEPLSKQIADVVTPLWNIPYEEQLNRKLREVEGVLQRLAREISNSNKAMLPWL-FVQKQKYNNMCCPLEGIRPSPVQTEYRNKCEFLIGTGANGEDKTIGFRLGKYKGGSCAVVGPSDTVHVPQEAKRVVTHFQDYI-------RTTKYSVYSPETYEGHWKQLTVRTSRTS-------------QIMAMVFFNPQKLAEEELNVLKSSIREHFTEGAGKESGVTSLYFVREGQRTSPNLEDLPCEHVAGEQWIHEELLGLKFRISPHSFFQINTPAAEVLYSTVGEWA---QLDQDS----TVLDVCCGTGTIGLSLAKRVKKVIGIELCQEAVEDAKVNAHINGVTNVEFHCGKAEDVFPSLLNAVISNK------LTAIVDPPRAGLHSKVILAIRRAEHLKRLVYVACNPKAAMNNFIDLCRAPSNRVRGAPFRPVRTMAVDLFPQTMHCEMLLLLERV 592
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. TrEMBL
Match: A0A4E0RVK1 (tRNA uracil 5 methyltransferase A OS=Fasciola hepatica OX=6192 GN=D915_007674 PE=3 SV=1) HSP 1 Score: 362.073 bits (928), Expect = 2.909e-113 Identity = 208/535 (38.88%), Postives = 303/535 (56.64%), Query Frame = 1
Query: 223 RDEAISKVTGHNWKGNVLLAKVAKGRADPFLQKR-KEMENVTEISDCKKIKLEN-----DDISNEEKLKDHVVKFWRKPYD-NQLIAKENKIKGILQNIFLKINKENILIKESKNFYSQINDNNV--VCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIGDTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLT-GKGKTCNVTSLLLTRRKNNSDPLDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHV-----IDSSSG---KHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYNNFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTRLA 1773
RD AI+ + G+ ++ L A++A GRADP LQKR ++ E D K+ LE D+ +L+D V WR YD +QL +K + L + KI N +K+ + ++ VCPL+ +IPSPVT+ YRNK E TIG LE GP+ GF KYR G A+G L I+P ++L + + Q E S + P T QGH R + +R + + L+++D+HP ++E+ L + D +F G G + +TSL L+ RK+ S+ D++ T+ ++G ++ E L FRIS +AFFQVNTLA E LY + +L + ID++S +HV L+DVCCGTGTIGL +A D+VIG+EM +V DA +N LN + A F+ GKAED++ +++ T + + A+VDPPRAGLH +V+Q LRRC + ++++VSCN EAA +NF++FARP S R G PFIP +A VDLFP T HVE ++LL R+
Sbjct: 123 RDAAITILDGYMFRNQKLEARLALGRADPLLQKRLPQVAAGEESVDRKRACLEPFDSDIKDVDEASRLRDTVTPLWRINYDPDQLDSKRASLLSSLAIVRCKIISCNSTLKKHLPHTDKCGEHERLPVCPLIELIPSPVTEGYRNKSELTIGCDLEGKGPLIGFRFTKYRDGLTAVGSYRHLNILPPSTVQMLDSLQKFIDQYSPCEPHERFSAYDPITHQGHWRQVLVRETRL-------------EDRLILLDVHPDGINEDSLNHLSDSLRTWFADDGPGNSVRITSLFLSTRKSTSERFDSSNTRLLYGEQYITELCCCLKFRISLDAFFQVNTLATELLYEQVAKLATESRNTSDDIDAASKIKREHV-LIDVCCGTGTIGLCLAKNFDRVIGIEMVSSAVADAKFNMVLNGVENARFYTGKAEDVMHDVVKTISEEAD-----ITAVVDPPRAGLHTTVVQVLRRCHRLHRIVYVSCNLEAAVHNFVEFARPTSNRFQGAPFIPILAKAVDLFPQTRHVEALILLERVG 638
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. TrEMBL
Match: A0A2J7PM02 (Uncharacterized protein OS=Cryptotermes secundus OX=105785 GN=B7P43_G02983 PE=3 SV=1) HSP 1 Score: 352.829 bits (904), Expect = 5.385e-110 Identity = 200/528 (37.88%), Postives = 296/528 (56.06%), Query Frame = 1
Query: 223 RDEAISKVTGHNWKGNVLLAKVAKGRADPFLQKRK-EMENVTEISDCKKIKLENDDISNEEKLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKENILIK----ESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIGDTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLR--TGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLL---LTRRKNNSD--PLDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYNNFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTRL 1770
R A+ + G WK L A++AK DP ++KR+ E ++ D KK K+ DD S EE+LK + FW PYD Q+ K+ I+ IL + + K N +K + ++ Y N + C L + SPV D YRNKCEFT+G E GF G Y GF +G CL IP +M + + +L VF P T +G+ R + +R TGS L++++ +HPQ LS+ EL+ + D ++F G+GKTCNV SL + RR+ + PL++ + G +YE L+ L FR+S AFFQ+NT AAE LY+++ +L S ++ T++D+CCGTG+IGL +A +V+GVE+ ++V+DA N + N+IT F G+AED++S++++ A K + +A+VDPPRAGLH + LRR E + K++F+SC+P+AA NF+D +RP SK HG P +P AVPVD+FPHT H EL+V L R+
Sbjct: 117 RQNALRALDGFVWKNKTLSAEIAKPAPDPLVKKRRQESHEKRDVEDAKKQKI--DDKSQEERLKASTIPFWDVPYDKQIEQKQANIRQILVKMGHDMVKGNPALKPWVDKQRSKY-----NGLPCELWDVRASPVCDGYRNKCEFTVGINEETGERTVGFRLGSYVQGFTGVGPVDCLRHIPDRMKEAAKTFEKFVQ-------ASDLGVFNPETHEGYWRQLMVRLSTGS--------------GQLMLVVGMHPQTLSQEELRKVKDDLKDFFENGEGKTCNVASLYFQHIMRRQAGKELPPLEH-----LMGKTHIYETLLGLKFRVSPEAFFQINTSAAEVLYNSVAEL-------SCPTENTTVIDICCGTGSIGLCLAKKCGQVLGVELLAQAVDDAKSNAKDNDITNCDFFVGRAEDILSSVMNRAVKG------DILAVVDPPRAGLHHRALIHLRRAEKLSKLVFLSCDPKAAAKNFVDLSRPTSKTYHGAPLVPVRAVPVDMFPHTSHCELIVYLERV 598
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. TrEMBL
Match: A0A3B3SFP7 (tRNA methyltransferase 2 homolog A OS=Paramormyrops kingsleyae OX=1676925 PE=3 SV=1) HSP 1 Score: 348.977 bits (894), Expect = 8.121e-109 Identity = 202/519 (38.92%), Postives = 286/519 (55.11%), Query Frame = 1
Query: 220 KRDEAISKVTGHNWKGNVLLAKVAKGRADPFLQKRKEMENVTEISDCKKIKLENDDISNEEKLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKENILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIGDTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTR--RKNNSDPLDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYNNFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTRL 1770
+RD A+ V G WKG VL ++AK +ADP L+KRK+ E E+ +S + + D V W PY+ QL K +++G+LQ + +I+ N + F + NN+ CPL I PSPV YRNKCEF IG GF GKY+GG A+ S +P + +V+ H D + T SV+ P T +GH + +T+RT + ++ ++ +PQ L+E EL + E+F G GK VTSL R ++ + N + + G +++E+L+ L FRIS ++FFQ+NT AAE LYST+ + +D S T+LDVCCGTGTIGL +A V KVIG+E+C E+VEDA N +N +T FH GKAED+ +LL+ ++ AIVDPPRAGLH VI A+RR E +K++++V+CNP+AA NNFID R S R G PF P + VDLFP T H E+++LL R+
Sbjct: 109 ERDRAMKAVHGMLWKGRVLSVRLAKPKADPILKKRKQEE------------AESKPLSKQ--IADVVTPLWNIPYEEQLNRKLREVEGVLQRLAREISNSNKAMLPWL-FVQKQKYNNMCCPLEGIRPSPVQTEYRNKCEFLIGTGANGEDKTIGFRLGKYKGGSCAVVGPSDTVHVPQEAKRVVTHFQDYIR-------TTKYSVYSPETYEGHWKQLTVRTSRTS-------------QIMAMVFFNPQKLAEEELNVLKSSIREHFTEGAGKESGVTSLYFVREGQRYQTTSYSNLPCEHVAGEQWIHEELLGLKFRISPHSFFQINTPAAEVLYSTVGEWA---QLDQDS----TVLDVCCGTGTIGLSLAKRVKKVIGIELCQEAVEDAKVNAHINGVTNVEFHCGKAEDVFPSLLNAVISNK------LTAIVDPPRAGLHSKVILAIRRAEHLKRLVYVACNPKAAMNNFIDLCRAPSNRVRGAPFRPVRTMAVDLFPQTMHCEMLLLLERV 579
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Cavefish
Match: trmt2a (tRNA methyltransferase 2 homolog A [Source:NCBI gene;Acc:103033829]) HSP 1 Score: 341.658 bits (875), Expect = 1.592e-108 Identity = 198/532 (37.22%), Postives = 283/532 (53.20%), Query Frame = 1
Query: 220 KRDEAISKVTGHNWKGNVLLAKVAKGRADPFLQKRKEMENVTEISD-CKKIKLENDDISNEEKLKDHVVK----FWRKPYDNQLIAKENKIKGILQNIFLKINKEN------ILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAI---GDTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTRR-KNNSDPLDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYNNFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTRL 1770
+RD A+ V G WKG VL ++AK +ADP ++KRK+ E K E D +E L + + + W+ PY+ QL KE +++ +LQN+ +I N + +++ KN N+ CPL PI PSP YRNKCEF IG + GF GKY+GG A+ DT P +++ + P +V+ P T +GH R +T+RT N ++ ++ +PQ LSE EL S+ +YF G G+ +TSL R + S ++ + + G +++E+L+ L FRIS ++FFQ NT AAE LYS + + +D S T+LDVCCGTGTIG+ +A V KVIG+E+C E+VEDA N + NN+T FH GKAED+ +L+ AIVDPPRAGLH VI A+RR E +K++++V+CN +AA NNF+D R S R G PF P A+ VDLFP T H E ++L R+
Sbjct: 134 ERDRAMKLVHGMQWKGKVLSVRLAKPKADPIVRKRKQDEGTQGGGQPVPKRPAEEQDAEDETPLSEQIARAVTPLWKIPYEEQLRRKEQEVETVLQNLAREIGNNNKAMLPWLFVQKEKN-------KNMCCPLEPIRPSPQQTEYRNKCEFVIGMGADGEDKTVGFRLGKYKGGSCAVVSPADTVHTPKSAIRVVQAFQRYIRTTP----------YAVYSPETYEGHWRQLTVRTSRTN-------------QIMAMVFFNPQKLSEEELGSLKKSLGQYFTEGDGRESRITSLHFVRMGQRTSAGTEDLPCELVAGDEWIHEELLGLKFRISPHSFFQTNTPAAEVLYSAVGEWA---QLDQES----TVLDVCCGTGTIGISLAKRVKKVIGIELCQEAVEDAKVNAKANNLTNVEFHCGKAEDIFPTVLNAVVSPN------VTAIVDPPRAGLHSKVILAIRRAEHLKRLVYVACNAKAAKNNFVDLCRAPSNRVRGSPFRPVRAMAVDLFPQTMHCETILLFERV 622 HSP 2 Score: 55.4546 bits (132), Expect = 1.225e-7 Identity = 29/58 (50.00%), Postives = 40/58 (68.97%), Query Frame = 3
Query: 54 YGYLDKGEFTSELFKIEIKNLHPKVGFSQLKQRLKSIGLNPHKVKITNEC--SFVTFK 221
Y Y+ + FTSE++K+EI+NL +GF+ LK+ L GLNPHK+KI +FVTFK
Sbjct: 73 YRYIKEDLFTSEIYKVEIQNLPRFIGFNDLKKFLNKHGLNPHKIKIVGRQTFAFVTFK 130
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Yeast
Match: TRM2 (tRNA methyltransferase; 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair [Source:SGD;Acc:S000001764]) HSP 1 Score: 73.559 bits (179), Expect = 3.328e-14 Identity = 59/213 (27.70%), Postives = 96/213 (45.07%), Query Frame = 1
Query: 1141 VYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSG---KHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYNNFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTRL 1770
V E + F S FFQ N ++ + + + D++ + G K L+D CG+G + + VDKVIGVE+ +SV A N + N + F GKAE + ++ + +E + I+DPPR G ++ L K+I++SCN + + F + + +G E D FP T HVE V ++ R+
Sbjct: 443 VTEYVDGYTFNFSAGEFFQNNN----SILPIVTKYVRDNLQAPAKGDDNKTKFLVDAYCGSGLFSICSSKGVDKVIGVEISADSVSFAEKNAKANGVENCRFIVGKAEKLFESIDTPSENTS--------VILDPPRKGCDELFLKQLAAYNPA-KIIYISCNVHSQARDVEYF---LKETENGSAHQIESIRGFDFFPQTHHVESVCIMKRI 639
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Nematostella
Match: EDO47401 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RLV8]) HSP 1 Score: 273.863 bits (699), Expect = 5.618e-83 Identity = 180/532 (33.83%), Postives = 264/532 (49.62%), Query Frame = 1
Query: 220 KRDEAISKVTGHNWKGNVLLAKVAKGRADPFLQKRKEMEN------VTEIS----DCKKIKLEND--DISNEEKLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKENILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIGDTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLL----TRRKNNSDPLDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYNNFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTR 1767
+R +A+ ++G W+G +L K+AK DP + K+K E + E + D K+ K D + +L D V W KPY QL K KI+ L+ + +I K+ + +K + N + C L SP+ D YRNKCEFTI + + GF G Y+ G+ + + P +SK + + I P + GH + +T+R+ + + I+ +PQ +S E+ + D YFL G+GK CN+TSL TR P T + G ++E + ++ F IS NAFFQVN+ AAE + I + + + K TLLD+CCGTG+IGL +A V KVIGVE+ +VEDA N +N IT A + GKAED + + + + + + I+DPPRAG+H SVI A+R+ + + K+I+VSC P+ A N +D RP SKR PF AVPVDLFPHT H EL++L R
Sbjct: 201 ERMKALKILSGSKWRGKILRTKLAKALKDPVVVKQKAAEGKERKRMIQEAAERNPDAKRAKTGGDKNQVDFFARLNDSVTPLWSKPYSEQLEFKNQKIEEFLKKLTKQI-KDMMELKGTWVEEESKVRNGICCELQKCEESPIVDGYRNKCEFTIAEGPKGAKTV-GFRVGSYKEGYSGVIE----PFNCCHISKTAKKVASAFQKYIRNSPLPEYDLC---FHTGHWQTLTVRSTVLG-------------ESMAIVQYNPQKMSATEIATENDKIRRYFLEGEGKECNLTSLYAQVNATRAVGCQSPAPFTH---LLGEKVIHEVICDVKFGISPNAFFQVNSKAAEKMIHIISRWMGEQ-------KETTLLDICCGTGSIGLCLAKYVSKVIGVEVIQSAVEDAKQNAIMNGITNAEYVCGKAED----VFTYKSMKRLRRFTQCIGIMDPPRAGVHKSVIDAIRKYDKLDKLIYVSCAPDHAIPNLLDLCRPTSKRVKSVPFRLVSAVPVDLFPHTKHCELLMLFQR 696
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Medaka
Match: trmt2a (tRNA methyltransferase 2 homolog A [Source:NCBI gene;Acc:101155696]) HSP 1 Score: 323.168 bits (827), Expect = 2.750e-102 Identity = 191/520 (36.73%), Postives = 276/520 (53.08%), Query Frame = 1
Query: 220 KRDEAISKVTGHNWKGNVLLAKVAKGRADPFLQKRKEM--ENVTEISDCKKIKLENDDISNEEKLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKENILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIGDTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTRRKNNSDP-LDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYNNFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTRL 1770
+RD+A V G WKG VL ++AK + DP L+KRK+ E K+ + + +++ ++ + V W Y+ QL KE ++ G+LQ + +I N + F ++ N + CPL I PSP YRNKCEF I + GF GKY+GG SC + P V ++ + T S + P T +GH + +T+RT T + E + ++ +PQ L E +L ++ +F G GK VTSL R P ++ + + G ++E+L+ L FRIS ++FFQVNT AAE LYST+ +D S T+LDVCCGTGTIG+ +A V KVIG+E+C E+VEDA N +LN ++ FH GKAED+ ++L+ A+VDPPRAGLH VI A+RR E +K++++V+CN +AA NFID R S R HG PF P A+ VDLFP T HVE+++L R+
Sbjct: 123 ERDKAHKLVHGMQWKGQVLSVRLAKPKEDPILKKRKQEVGEGGAGQPASKRAEGDEEELPLSVQIANVVTPLWNSTYEEQLKMKEQEVVGVLQKLAKEIGSTNKAMLPWL-FAQKVKHNKMCCPLEAIRPSPTQTEYRNKCEFLISVGADGEDKTIGFRLGKYKGG-------SCAVVGPSATCHVSAAAKTVVREFQKFIRTTPYSAYSPETYEGHWKQLTVRT---------TRAKE----AMAVVFFNPQKLEEEDLNALKTSMRTFFTEGDGKDSGVTSLYFVRDGQRKSPNPEDLPCELVAGESCIHEELLGLRFRISPHSFFQVNTAAAEVLYSTVGDWA---QLDQDS----TVLDVCCGTGTIGISLAKRVKKVIGIELCQEAVEDAKVNAKLNGLSNVEFHCGKAEDVFPDILNALVSPS------LTAVVDPPRAGLHPKVILAIRRAEHLKRLVYVACNAKAAMTNFIDLCRAPSNRVHGSPFRPVRAMAVDLFPQTIHVEMLLLFERV 608 HSP 2 Score: 51.2174 bits (121), Expect = 2.673e-6 Identity = 27/63 (42.86%), Postives = 42/63 (66.67%), Query Frame = 3
Query: 39 STNNPYGYLDKGEFTSELFKIEIKNLHPKVGFSQLKQRLKSIGLNPHKVKITNEC--SFVTFK 221
S + Y Y+ + FTSE++K+EI+NL +GF+ LK+ L +NPHK+K+ + +FVTFK
Sbjct: 57 SDPDKYRYIKEDLFTSEIYKVEIRNLPKFIGFNDLKKFLAKHSINPHKIKLIGKHTFAFVTFK 119
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Medaka
Match: trmt2b (tRNA methyltransferase 2 homolog B [Source:NCBI gene;Acc:101156035]) HSP 1 Score: 244.973 bits (624), Expect = 7.973e-74 Identity = 161/474 (33.97%), Postives = 235/474 (49.58%), Query Frame = 1
Query: 361 KKIKLENDDISNEEKLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKENILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIGDTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTRRKNNSDPLDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSS-SGKHV-TLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEA-AYNNFIDFARP--ISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTR 1767
+K L +D++S EE+L D V WR YD QL K+ + L + ++ + KN + PLLPI+PSPV D YRNK F++ + GF G + G + L +P + V D + Q P L +F G GH R +T+RT + + I+ HPQ L+ E+ +YF+ G G C + SL + + + ++G +YE ++ FRIS +AFFQVN AAE LY T+R L+ + GK TLLDVCCGTG IG+ + V+KVIG+E+ ++VEDA +N LNN+ F +GKAE ++ L+ + A+V+P RAGLH V++ALR I+++++VSC P+ A NF + P K+ G F P +A+PVD+FPHTPH ELV+L R
Sbjct: 41 QKNHLWSDNLSWEERLADAVTPLWRLSYDEQLELKQKHQETTLSQLVGHLSGDPKSSSPVKN--------KLTFPLLPILPSPVRDGYRNKSTFSVNRGADGNTKTVGFYLGTGKQGNIVCVNGDHLLNMPEKHKLVARCYQDFIRQ---SPLDPCL-LFHSG---GHWREVTVRTNATG-------------HTMAIVYFHPQTLTPEEVAVHQTDLVDYFMRGPGSVCQLDSLFFQESSMTRCTHEKSPYQLLYGQPHIYETVLGFKFRISPDAFFQVNQGAAEVLYGTLRDLISANTEKGEKQGKPTDTLLDVCCGTGAIGITTSPRVEKVIGIELIEQAVEDAVHNAALNNVLNCKFISGKAEAVLPGLIVDLSCTGGG----LTAVVNPARAGLHYRVVRALRNQPNIRRLVYVSCKPDGEAMRNFRELCCPPDPQKKLTGEAFSPILAIPVDMFPHTPHCELVLLFER 482
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Medaka
Match: trmt2a (tRNA methyltransferase 2 homolog A [Source:NCBI gene;Acc:101155696]) HSP 1 Score: 244.973 bits (624), Expect = 3.582e-73 Identity = 154/447 (34.45%), Postives = 226/447 (50.56%), Query Frame = 1
Query: 220 KRDEAISKVTGHNWKGNVLLAKVAKGRADPFLQKRKEM--ENVTEISDCKKIKLENDDISNEEKLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKENILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIGDTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTRRKNNSDP-LDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGL 1551
+RD+A V G WKG VL ++AK + DP L+KRK+ E K+ + + +++ ++ + V W Y+ QL KE ++ G+LQ + +I N + F ++ N + CPL I PSP YRNKCEF I + GF GKY+GG SC + P V ++ + T S + P T +GH + +T+RT T + E + ++ +PQ L E +L ++ +F G GK VTSL R P ++ + + G ++E+L+ L FRIS ++FFQVNT AAE LYST+ +D S T+LDVCCGTGTIG+ +A V KVIG+E+C E+VEDA N +LN ++ FH GKAED+ ++L+ A+VDPPRAGL
Sbjct: 123 ERDKAHKLVHGMQWKGQVLSVRLAKPKEDPILKKRKQEVGEGGAGQPASKRAEGDEEELPLSVQIANVVTPLWNSTYEEQLKMKEQEVVGVLQKLAKEIGSTNKAMLPWL-FAQKVKHNKMCCPLEAIRPSPTQTEYRNKCEFLISVGADGEDKTIGFRLGKYKGG-------SCAVVGPSATCHVSAAAKTVVREFQKFIRTTPYSAYSPETYEGHWKQLTVRT---------TRAKE----AMAVVFFNPQKLEEEDLNALKTSMRTFFTEGDGKDSGVTSLYFVRDGQRKSPNPEDLPCELVAGESCIHEELLGLRFRISPHSFFQVNTAAAEVLYSTVGDWA---QLDQDS----TVLDVCCGTGTIGISLAKRVKKVIGIELCQEAVEDAKVNAKLNGLSNVEFHCGKAEDVFPDILNALVSPS------LTAVVDPPRAGL 535 HSP 2 Score: 50.8322 bits (120), Expect = 3.582e-73 Identity = 27/63 (42.86%), Postives = 42/63 (66.67%), Query Frame = 3
Query: 39 STNNPYGYLDKGEFTSELFKIEIKNLHPKVGFSQLKQRLKSIGLNPHKVKITNE--CSFVTFK 221
S + Y Y+ + FTSE++K+EI+NL +GF+ LK+ L +NPHK+K+ + +FVTFK
Sbjct: 57 SDPDKYRYIKEDLFTSEIYKVEIRNLPKFIGFNDLKKFLAKHSINPHKIKLIGKHTFAFVTFK 119
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Planmine SMEST
Match: SMESG000008614.1 (SMESG000008614.1) HSP 1 Score: 1070.84 bits (2768), Expect = 0.000e+0 Identity = 519/520 (99.81%), Postives = 520/520 (100.00%), Query Frame = 1
Query: 220 KRDEAISKVTGHNWKGNVLLAKVAKGRADPFLQKRKEMENVTEISDCKKIKLENDDISNEEKLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKENILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIGDTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTRRKNNSDPLDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYNNFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTRLAPL 1779
+RDEAISKVTGHNWKGNVLLAKVAKGRADPFLQKRKEMENVTEISDCKKIKLENDDISNEEKLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKENILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIGDTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTRRKNNSDPLDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYNNFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTRLAPL
Sbjct: 68 ERDEAISKVTGHNWKGNVLLAKVAKGRADPFLQKRKEMENVTEISDCKKIKLENDDISNEEKLKDHVVKFWRKPYDNQLIAKENKIKGILQNIFLKINKENILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIGDTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQGHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTGKGKTCNVTSLLLTRRKNNSDPLDNTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYNNFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTRLAPL 587 HSP 2 Score: 133.65 bits (335), Expect = 7.083e-33 Identity = 64/65 (98.46%), Postives = 65/65 (100.00%), Query Frame = 3
Query: 30 MESSTNNPYGYLDKGEFTSELFKIEIKNLHPKVGFSQLKQRLKSIGLNPHKVKITNECSFVTFKT 224
MESSTNNPYGYLDKGEFTSELFKIEIKNLHPKVGFSQLKQRLKSIGLNPHKVKITNECSFVTFK+
Sbjct: 1 MESSTNNPYGYLDKGEFTSELFKIEIKNLHPKVGFSQLKQRLKSIGLNPHKVKITNECSFVTFKS 65
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Planmine SMEST
Match: SMESG000077010.1 (SMESG000077010.1) HSP 1 Score: 159.844 bits (403), Expect = 7.480e-43 Identity = 129/448 (28.79%), Postives = 212/448 (47.32%), Query Frame = 1
Query: 439 PYDNQLIAKENKIKGILQNIFLKINKENILIKESKNFYSQINDNNVVCPLLPIIPSPVTDCYRNKCEFTIGFTLENTGPIAGFCHGKYRGGFDAIGDTSCLPIIPMQMSKVLHHINDILPQLISGEFTPNLSVFKPGTDQ--GHLRGITLRTGSINLDKSPTDSSEDIQSLLMIIDIHPQLLSENELQSIGDFFAEYFLTG-KGKTCNVTSLLLTRRKNNSDPLD-NTTTKTIFGSGFVYEKLMNLNFRISYNAFFQVNTLAAETLYSTIRQLLIDHVIDSSSGKHVTLLDVCCGTGTIGLVMADLVDKVIGVEMCPESVEDANYNKELNNITKATFHAGKAEDMISNLLSTAEKSQENNYREFVAIVDPPRAGLHISVIQALRRCELIKKVIFVSCNPEAAYN-NFIDFARPISKRTHGCPFIPEIAVPVDLFPHTPHVELVVLLTR 1767
PYD QLI K+ KI +QNI + K N L E + S+ + ++C + I SPV + YRNK +GF E G H FD T + L + IL + +F S F P + +GIT+R+ N ++ I + + + I ++ FLT + ++ NV SL + +++ + + K I+G ++ E + F IS +FFQVN AE +Y +QL+ D+ S SGK + LLD+CCGTGT+G++++D+V++VIGV++ P ++E+A +N + NN+ E+ + L++ + +N VA+++P R GL + LR E +K ++++SC+ E N PF +P+D FPHT HVE++ +TR
Sbjct: 15 PYDAQLITKQYKIFSAMQNISSLLRKSNTLSPEILEYCSK--NKGLLCKIEDIYKSPVIEGYRNKMGAIVGFD-EMGNLTVG--HAMTFSNFDK---TRKKLVTFSHFCDKLDKKHHILIAKLFEKFIKEDSQFIPNQKKKISLYKGITIRSNRKN-------------ETMINIHFRARFTGIDRILGISKELSDQFLTSLQNESINVRSLYCQQMESHFEHTHAQLSCKLIYGDKYLMENVCGKTFYISPLSFFQVNVEVAEMIYQKAKQLVEDY--KSKSGKKILLLDLCCGTGTLGIILSDIVEQVIGVDIVPSAIENAKFNAKANNVNNVEHFCKPVEEFLPEFLNSDIAKEYDN---VVAVLNPSRDGLSTEFCETLRSFESLKMIVYISCHVEGQTTLNICTLCTVKGDSNPEVPFSLSKIIPLDPFPHTTHVEVIFQMTR 436 The following BLAST results are available for this feature:
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 1
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 1
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 1
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 3
BLAST of tRNA (Uracil-5-)-methyltransferase homolog A vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 2
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30000955 ID=SMED30000955|Name=tRNA (Uracil-5-)-methyltransferase homolog A|organism=Schmidtea mediterranea sexual|type=transcript|length=1788bpback to top protein sequence of SMED30000955-orf-1 >SMED30000955-orf-1 ID=SMED30000955-orf-1|Name=SMED30000955-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=484bp MENVTEISDCKKIKLENDDISNEEKLKDHVVKFWRKPYDNQLIAKENKIKback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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