Tgs-1
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30000949 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 8
Homology
BLAST of Tgs-1 vs. TrEMBL
Match: A0A2Z4ELZ5 (Tgs-1 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 901.738 bits (2329), Expect = 0.000e+0 Identity = 451/451 (100.00%), Postives = 451/451 (100.00%), Query Frame = 1 Query: 82 MEQIYFNDFLSSCGASSTPIMKIHRQNLGVDKDNNIPLTNKELVIQLLEAKEEWPMAFSRVTQNRLNELKLGFDEDFLLKQHEQRKPRRMSPMIINHNRNCGRNSNRDRSMSPSGAIKMKESKWERRSEIAMLLIDHILMKFEDLPLNFLKDQPIWKTQSKQLIDTLGKSIENKTKIEKIRESNLHIIGQSLRIILNKMGMLFPKVISEQILQLKFLRTRSGNAIVKDRSKIEAVQILLLHISTPNRILINKLLEMFRIISKIRKIQMSGEELGKILCQSFFEELNSESIKLCKDMIVLVEHLFLAPLGILSALKVSINTRTAPTNLIIDFPEKKLKLNKPISTPTKPNIKIFHKPTTPITKSISGVPIVSFFPNSKWNSQQESVILTAFNKINSPEQIKKPSVPMIFTPTLKNQTLKRKIIVKSLSPLKWNKKCIVPNLNEFKKRKLAAN 1434 MEQIYFNDFLSSCGASSTPIMKIHRQNLGVDKDNNIPLTNKELVIQLLEAKEEWPMAFSRVTQNRLNELKLGFDEDFLLKQHEQRKPRRMSPMIINHNRNCGRNSNRDRSMSPSGAIKMKESKWERRSEIAMLLIDHILMKFEDLPLNFLKDQPIWKTQSKQLIDTLGKSIENKTKIEKIRESNLHIIGQSLRIILNKMGMLFPKVISEQILQLKFLRTRSGNAIVKDRSKIEAVQILLLHISTPNRILINKLLEMFRIISKIRKIQMSGEELGKILCQSFFEELNSESIKLCKDMIVLVEHLFLAPLGILSALKVSINTRTAPTNLIIDFPEKKLKLNKPISTPTKPNIKIFHKPTTPITKSISGVPIVSFFPNSKWNSQQESVILTAFNKINSPEQIKKPSVPMIFTPTLKNQTLKRKIIVKSLSPLKWNKKCIVPNLNEFKKRKLAAN Sbjct: 1 MEQIYFNDFLSSCGASSTPIMKIHRQNLGVDKDNNIPLTNKELVIQLLEAKEEWPMAFSRVTQNRLNELKLGFDEDFLLKQHEQRKPRRMSPMIINHNRNCGRNSNRDRSMSPSGAIKMKESKWERRSEIAMLLIDHILMKFEDLPLNFLKDQPIWKTQSKQLIDTLGKSIENKTKIEKIRESNLHIIGQSLRIILNKMGMLFPKVISEQILQLKFLRTRSGNAIVKDRSKIEAVQILLLHISTPNRILINKLLEMFRIISKIRKIQMSGEELGKILCQSFFEELNSESIKLCKDMIVLVEHLFLAPLGILSALKVSINTRTAPTNLIIDFPEKKLKLNKPISTPTKPNIKIFHKPTTPITKSISGVPIVSFFPNSKWNSQQESVILTAFNKINSPEQIKKPSVPMIFTPTLKNQTLKRKIIVKSLSPLKWNKKCIVPNLNEFKKRKLAAN 451
BLAST of Tgs-1 vs. Planmine SMEST
Match: SMESG000058876.1 (SMESG000058876.1) HSP 1 Score: 848.195 bits (2190), Expect = 0.000e+0 Identity = 430/451 (95.34%), Postives = 431/451 (95.57%), Query Frame = 1 Query: 82 MEQIYFNDFLSSCGASSTPIMKIHRQNLGVDKDNNIPLTNKELVIQLLEAKEEWPMAFSRVTQNRLNELKLGFDEDFLLKQHEQRKPRRMSPMIINHNRNCGRNSNRDRSMSPSGAIKMKESKWERRSEIAMLLIDHILMKFEDLPLNFLKDQPIWKTQSKQLIDTLGKSIENKTKIEKIRESNLHIIGQSLRIILNKMGMLFPKVISEQILQLKFLRTRSGNAIVKDRSKIEAVQILLLHISTPNRILINKLLEMFRIISKIRKIQMSGEELGKILCQSFFEELNSESIKLCKDMIVLVEHLFLAPLGILSALKVSINTRTAPTNLIIDFPEKKLKLNKPISTPTKPNIKIFHKPTTPITKSISGVPIVSFFPNSKWNSQQESVILTAFNKINSPEQIKKPSVPMIFTPTLKNQTLKRKIIVKSLSPLKWNKKCIVPNLNEFKKRKLAAN 1434 MEQIYFNDFLSSCGASSTPIMKIHRQNLGVDKDNNI LTNKELVIQLLEAKEEWPMAFSRVTQNRLNELKLG DEDFLLKQHEQRKPRRMSPMIINHNRNCGRNSNRDRSMSPSGAIKMKESKWERRSEIAMLLIDHILMKFEDLPLNFLKDQPIWKTQSKQLIDTLGKSIENKTKIEKIRESNLHIIGQSLRIILNKMGMLFPKVISEQILQLKFLRTRSGNAIVKDRSKIEAVQILLLHISTPNRILINKLLEMFRIISKIRKIQMSGEELGKILCQSFFEELNSE+ PLGILSALKVSINTRT PTNLIIDFPEKKLKLNKPISTPTKPNIKIFHKPTTPITKSISGVPIVSFFPNSKWNSQQESVILTAFNKINSPEQIKKPSVPMIFTPTLKNQTLKRKIIVKSLSPLKWNKKCIVPNLNEFKKRKLAAN Sbjct: 1 MEQIYFNDFLSSCGASSTPIMKIHRQNLGVDKDNNILLTNKELVIQLLEAKEEWPMAFSRVTQNRLNELKLGLDEDFLLKQHEQRKPRRMSPMIINHNRNCGRNSNRDRSMSPSGAIKMKESKWERRSEIAMLLIDHILMKFEDLPLNFLKDQPIWKTQSKQLIDTLGKSIENKTKIEKIRESNLHIIGQSLRIILNKMGMLFPKVISEQILQLKFLRTRSGNAIVKDRSKIEAVQILLLHISTPNRILINKLLEMFRIISKIRKIQMSGEELGKILCQSFFEELNSET-----------------PLGILSALKVSINTRTTPTNLIIDFPEKKLKLNKPISTPTKPNIKIFHKPTTPITKSISGVPIVSFFPNSKWNSQQESVILTAFNKINSPEQIKKPSVPMIFTPTLKNQTLKRKIIVKSLSPLKWNKKCIVPNLNEFKKRKLAAN 434
BLAST of Tgs-1 vs. Planmine SMEST
Match: SMESG000058876.1 (SMESG000058876.1) HSP 1 Score: 836.639 bits (2160), Expect = 0.000e+0 Identity = 426/451 (94.46%), Postives = 427/451 (94.68%), Query Frame = 1 Query: 82 MEQIYFNDFLSSCGASSTPIMKIHRQNLGVDKDNNIPLTNKELVIQLLEAKEEWPMAFSRVTQNRLNELKLGFDEDFLLKQHEQRKPRRMSPMIINHNRNCGRNSNRDRSMSPSGAIKMKESKWERRSEIAMLLIDHILMKFEDLPLNFLKDQPIWKTQSKQLIDTLGKSIENKTKIEKIRESNLHIIGQSLRIILNKMGMLFPKVISEQILQLKFLRTRSGNAIVKDRSKIEAVQILLLHISTPNRILINKLLEMFRIISKIRKIQMSGEELGKILCQSFFEELNSESIKLCKDMIVLVEHLFLAPLGILSALKVSINTRTAPTNLIIDFPEKKLKLNKPISTPTKPNIKIFHKPTTPITKSISGVPIVSFFPNSKWNSQQESVILTAFNKINSPEQIKKPSVPMIFTPTLKNQTLKRKIIVKSLSPLKWNKKCIVPNLNEFKKRKLAAN 1434 MEQIYFNDFLSSCGASSTPIMKIHRQNLGVDKDNNI LTNKELVIQLLEAKEEWPMAFSRVTQNRLNELKLG DEDFLLKQHEQRKPRRMSPMIINHNRNCGRNSNRDRSMSPSGAIKMKESKWERRSEIAMLLIDHILMKFEDLPLNFLKDQPIWKTQSKQLIDTLGKSIENKTKIEKIRESNLHIIGQSLRIILNKMGMLFPKVISEQILQLKFLRTRSGNAIVKDRSKIEAVQILLLHISTPNRILINKLLEMFRIISKIRKIQMSGEELGKILCQSFFEELNSE+ PLGILSALKVSINTRT PTNLIIDFPEKKLKLNKPISTPTKPNIKIFHKPTTPITKSISGVPIVSFFPNSKWNSQQESVILTAFNKINSPEQIKKPSVPMIFTPTLKNQTLKR KSLSPLKWNKKCIVPNLNEFKKRKLAAN Sbjct: 1 MEQIYFNDFLSSCGASSTPIMKIHRQNLGVDKDNNILLTNKELVIQLLEAKEEWPMAFSRVTQNRLNELKLGLDEDFLLKQHEQRKPRRMSPMIINHNRNCGRNSNRDRSMSPSGAIKMKESKWERRSEIAMLLIDHILMKFEDLPLNFLKDQPIWKTQSKQLIDTLGKSIENKTKIEKIRESNLHIIGQSLRIILNKMGMLFPKVISEQILQLKFLRTRSGNAIVKDRSKIEAVQILLLHISTPNRILINKLLEMFRIISKIRKIQMSGEELGKILCQSFFEELNSET-----------------PLGILSALKVSINTRTTPTNLIIDFPEKKLKLNKPISTPTKPNIKIFHKPTTPITKSISGVPIVSFFPNSKWNSQQESVILTAFNKINSPEQIKKPSVPMIFTPTLKNQTLKR----KSLSPLKWNKKCIVPNLNEFKKRKLAAN 430
BLAST of Tgs-1 vs. Planmine SMEST
Match: SMESG000058876.1 (SMESG000058876.1) HSP 1 Score: 803.897 bits (2075), Expect = 0.000e+0 Identity = 410/431 (95.13%), Postives = 411/431 (95.36%), Query Frame = 1 Query: 142 MKIHRQNLGVDKDNNIPLTNKELVIQLLEAKEEWPMAFSRVTQNRLNELKLGFDEDFLLKQHEQRKPRRMSPMIINHNRNCGRNSNRDRSMSPSGAIKMKESKWERRSEIAMLLIDHILMKFEDLPLNFLKDQPIWKTQSKQLIDTLGKSIENKTKIEKIRESNLHIIGQSLRIILNKMGMLFPKVISEQILQLKFLRTRSGNAIVKDRSKIEAVQILLLHISTPNRILINKLLEMFRIISKIRKIQMSGEELGKILCQSFFEELNSESIKLCKDMIVLVEHLFLAPLGILSALKVSINTRTAPTNLIIDFPEKKLKLNKPISTPTKPNIKIFHKPTTPITKSISGVPIVSFFPNSKWNSQQESVILTAFNKINSPEQIKKPSVPMIFTPTLKNQTLKRKIIVKSLSPLKWNKKCIVPNLNEFKKRKLAAN 1434 MKIHRQNLGVDKDNNI LTNKELVIQLLEAKEEWPMAFSRVTQNRLNELKLG DEDFLLKQHEQRKPRRMSPMIINHNRNCGRNSNRDRSMSPSGAIKMKESKWERRSEIAMLLIDHILMKFEDLPLNFLKDQPIWKTQSKQLIDTLGKSIENKTKIEKIRESNLHIIGQSLRIILNKMGMLFPKVISEQILQLKFLRTRSGNAIVKDRSKIEAVQILLLHISTPNRILINKLLEMFRIISKIRKIQMSGEELGKILCQSFFEELNSE+ PLGILSALKVSINTRT PTNLIIDFPEKKLKLNKPISTPTKPNIKIFHKPTTPITKSISGVPIVSFFPNSKWNSQQESVILTAFNKINSPEQIKKPSVPMIFTPTLKNQTLKRKIIVKSLSPLKWNKKCIVPNLNEFKKRKLAAN Sbjct: 1 MKIHRQNLGVDKDNNILLTNKELVIQLLEAKEEWPMAFSRVTQNRLNELKLGLDEDFLLKQHEQRKPRRMSPMIINHNRNCGRNSNRDRSMSPSGAIKMKESKWERRSEIAMLLIDHILMKFEDLPLNFLKDQPIWKTQSKQLIDTLGKSIENKTKIEKIRESNLHIIGQSLRIILNKMGMLFPKVISEQILQLKFLRTRSGNAIVKDRSKIEAVQILLLHISTPNRILINKLLEMFRIISKIRKIQMSGEELGKILCQSFFEELNSET-----------------PLGILSALKVSINTRTTPTNLIIDFPEKKLKLNKPISTPTKPNIKIFHKPTTPITKSISGVPIVSFFPNSKWNSQQESVILTAFNKINSPEQIKKPSVPMIFTPTLKNQTLKRKIIVKSLSPLKWNKKCIVPNLNEFKKRKLAAN 414
BLAST of Tgs-1 vs. Planmine SMEST
Match: SMESG000023662.1 (SMESG000023662.1) HSP 1 Score: 136.346 bits (342), Expect = 5.506e-35 Identity = 120/427 (28.10%), Postives = 214/427 (50.12%), Query Frame = 1 Query: 160 NLGVDKDNNIPLTNKELVIQLLEAKEEWPMAFSRVTQNRLNELKLGFDEDFLLKQHEQRKPRRMSPMIINHNRNCGRNSNRDRSMSPSGAIKMKESKWERRSEIAMLLIDHILMKFEDLPLNFLKDQPIWKTQSKQLIDTLGKSIENKTKIEKIRESNLHIIGQSLRIILNKMGMLFPKVISEQILQLKFLRTRSGNAIVKDRSKIEAVQILLLHISTPNRILINKLLEMFRIISKIRKIQMSGEELGKILCQSFFE-ELNSESIKLCKDMIVLVEHLFLAPL---GILSALKVSINTRTAPTNLIIDFPEKKLKLNKPISTPTKPNIKIFHKPTTPITKSISGVPIVSFFPNSKWNSQQES--VILTAFNKINS---PEQIKKPSVPMIFTPTLKNQTLKRKIIVKSLSPLKWNKKCIVPNLNEFK 1413 N+G + +++ ++ ++ +K +P AF +++Q LN L L D+ FL + ++ R ++N R R R RSMSPSG++ E WE EIA I+++L + LP+NF+ + K + K+++D L ++ K E ++ L +IG++++ I+ K+G+L P IS +I LK L N I K +S I AVQILLL+I +R L+ K++++F I++ + + E+L ++L S F+ E N ++L KD I L H+F P I++ ++I P LI K T P KS + I +F +SK N + + +++ + +K ++ P + +P + T++N+T++R I K S +K K+ + ++ FK Sbjct: 14 NVGGPPSDRCNQSSQSMMEYVVRSKNLFPTAFKKISQELLNRLDLELDKSFLPELTAGKRSRSRIASVVNK-RIPSRT--RQRSMSPSGSLNKAEKSWENNFEIANATIEYLLENIDLLPINFMNYSNMDK-EVKRVLDDLKGIKLSERKTEYLKRQKLFVIGETMKTIIQKLGVLLPAKISTEIESLKSLTCLKNNKIKKQKSHILAVQILLLNIPEQSRTLLQKIIKLFNILANHAALNRNKEQLYELLKLSLFDGENNYNKLQLSKDFINLSNHIFDCPSELHKIVNIYMIAIVNSNQPCELI--------------------------KGTEPSNKSTNST-IDCYFSHSKINRETNADLLLVDSSSKADAKLPPIKSGNGKIPQL---TMENKTVRRLSIRKKRSFVKTAKQVLTKTMSPFK 406
BLAST of Tgs-1 vs. Planmine SMEST
Match: SMESG000062596.1 (SMESG000062596.1) HSP 1 Score: 103.605 bits (257), Expect = 1.725e-23 Identity = 91/259 (35.14%), Postives = 145/259 (55.98%), Query Frame = 1 Query: 220 LLEAKEEWPMAFSRVTQNRLNELKLGFDEDFLLKQHEQRKPRRMSPMIINHNRNCGRNSNRDRSMSPSGAIKMKESKWERRSEIAMLLIDHILMKFEDLPLNFLKDQPIWKTQSKQLIDTLGKSIENKTKIEKIRESNLHIIGQSLRIILNKMGMLFPKVISEQILQLKFLRTRSGNAIVKDRSKIEAVQILLLHISTPNRILINKLLEMFRIISKIRKIQMSGEELGKILCQSFFE-ELNSESIKLCKDMIVLVEHLF 993 +LE K +P AF +V Q LN+L L D+ FL + + R +IN R+ +R RS+SPSG++ E WE E+A +I+ I LP NFL +QP + K L+D L + + E+++ + L ++G++++ IL K+G+L P IS +I LK LR N + K +S I A QILLL+I +R LI K++E+F +I + + + ++ E+L ++L S F+ E N ++L KD I L +F Sbjct: 31 VLECKFFYPSAFRKVCQELLNQLNLQLDKSFLTELKIVTRSRSKLAKVINSRT---RSRSRQRSISPSGSLNKTEKSWESNIEVACAIIEDIRENINLLPENFL-NQPGVDKEVKMLLDDLKVIKTTEKRRERLKRNELTVVGEAMKKILAKLGVLLPTRISTEIELLKSLRCFQNNQLKKRKSHILATQILLLNIPEQSRNLIQKIVELFNMILEHKTVNVNEEQLYELLKISLFDGENNYSKLQLSKDFIHLSNRIF 285 The following BLAST results are available for this feature:
BLAST of Tgs-1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of Tgs-1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of Tgs-1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Tgs-1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of Tgs-1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of Tgs-1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of Tgs-1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of Tgs-1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 1
BLAST of Tgs-1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of Tgs-1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Tgs-1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Tgs-1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of Tgs-1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of Tgs-1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30000949 ID=SMED30000949|Name=Tgs-1|organism=Schmidtea mediterranea sexual|type=transcript|length=1610bpback to top protein sequence of SMED30000949-orf-1 >SMED30000949-orf-1 ID=SMED30000949-orf-1|Name=SMED30000949-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=452bp MEQIYFNDFLSSCGASSTPIMKIHRQNLGVDKDNNIPLTNKELVIQLLEAback to top Annotated Terms
The following terms have been associated with this transcript:
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