Calpain-B
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30000946 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 8
Homology
BLAST of Calpain-B vs. Ensembl Human
Match: CAPN9 (calpain 9 [Source:HGNC Symbol;Acc:HGNC:1486]) HSP 1 Score: 573.548 bits (1477), Expect = 0.000e+0 Identity = 292/638 (45.77%), Postives = 417/638 (65.36%), Query Frame = 1
Query: 403 FYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEA-DPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILDVSFK-RENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYI 2310
F ++ + +LFED FPA+N+SL+Y E P WKRP E+ +N FI G +R D+ QGELGDCWLLAA++SL++ + +VIP DQ F Y G F F+ WQ W DVV+DDRLPT R RLV+LHS D NEFWSALLEKAYAK+ GSYEALKGGS+ EAMEDFTGG+ E F AP N ++I+ K + R SL+GC I+ + EA+ GLIKGHAYS+T + V+ + R+ L+R+RNPWG EW G++SD SPEW + E+KRL T DDGEFWM F+DF +F K+EIC+L+P++ LE A KWE+ + G W + +AGGCRNF++TF NPQ K+ + + DE E + ++ LMQK+ R ++ G ++ TIGYAIY+ D L+K +F+ AS A+S TFINLREV R KLP G+Y+++PSTF +EA F LRIFSE+ ++ +D +I D + P +E + + F+ ++G D E+ A+EL+ +L+ + +++ KF S +C++++++MDT +G+L F+EFKV WD L+ W +F +FD D+SGT ++YE+R AL GF++S+ + I+ RYA+++ + FDD++
Sbjct: 29 FEQMRQECLQRGTLFEDADFPASNSSLFYSERPQIPFVWKRPGEIVKNPEFILGGATRTDICQGELGDCWLLAAIASLTLNQKALARVIPQDQSFGPGYAGIFHFQFWQHSEWLDVVIDDRLPTFRDRLVFLHSADHNEFWSALLEKAYAKLNGSYEALKGGSAIEAMEDFTGGVAETFQT-KEAPENFYEILEKALKRGSLLGCFIDTRSAAESEARTPFGLIKGHAYSVTGIDQVSFRGQRIELIRIRNPWGQ-VEWNGSWSDSSPEWRSVGPAEQKRLCHTALDDGEFWMAFKDFKAHFDKVEICNLTPDA-LEEDA---IHKWEVTVHQGSWVRGSTAGGCRNFLDTFWTNPQIKLSLTEKDEGQEE--CSFLVALMQKDRRKLKRFGANVLTIGYAIYECPDK---DEHLNKDFFRYHASRARSKTFINLREVSDRFKLPPGEYILIPSTFEPHQEADFCLRIFSEKKAITRDMDGNVDI---DLPEPPKPTPPDQETEEEQRFRALFEQVAGEDMEVTAEELEYVLNAVLQKKKDIKFKKLSLISCKNIISLMDTSGNGKLEFDEFKVFWDKLKQWINLFLRFDADKSGTMSTYELRTALKAAGFQLSSHLLQLIVLRYADEELQLDFDDFL 652
BLAST of Calpain-B vs. Ensembl Human
Match: CAPN3 (calpain 3 [Source:HGNC Symbol;Acc:HGNC:1480]) HSP 1 Score: 570.466 bits (1469), Expect = 0.000e+0 Identity = 299/669 (44.69%), Postives = 436/669 (65.17%), Query Frame = 1
Query: 433 KNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEA-DPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNEN--TGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQM-KDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIP-PEVKREEDANNVKLQNAFKVISGADGEIDADELQQILD-VSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYIXXXXXXXXXXXXXKAQQKTSKGEAIFPQDEFMQCVLY 2421
K L+ DP FP + TSL+Y + P + WKRP E+ +N FI DG +R D+ QGELGDCW LAA++ L++ L+ +VIP DQ F +Y G F F+ W++G W DVV+DD LPT +LV+ S NEFWSALLEKAYAK+ GSYEALKGG+++EAMEDFTGG+ E F++ AP +++ IM K + R SLMGCSI+ P + E + GL++GHAYS+T + V K +V LVR+RNPWG EW G++SD+ +W+ + E+ RL +DGEFWM+++DF+ +FTKLEIC+L+ ++ +K W + + G W + SAGGCRNF +TF NPQ+++K+++ D++ +++ + ++ LMQK R RK G LFTIG+AIY++ K+ +K L K +F AS A+S T+IN+REV R +LP +YVIVPST+ +E +F+LR+FSE+ S++++ + P +P P +E + +N FK I+G D EI ADEL+++L+ V K ++ K +GF+ E+CRSM+A+MDTD SG+L +EF LW+ ++ W+ +FK +D DQSGT NSYE+R A+N GF ++N+++ I RYA+K I FD +I VRL +F F A K G E++Q +Y
Sbjct: 71 KKVLYVDPEFPPDETSLFYSQKFPIQFVWKRPPEICENPRFIIDGANRTDICQGELGDCWFLAAIACLTLNQHLLFRVIPHDQSFIENYAGIFHFQFWRYGEWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVAEFFEI-RDAPSDMYKIMKKAIERGSLMGCSIDTIIPVQYETRMACGLVRGHAYSVTGLDEVPFKGEKVKLVRLRNPWGQ-VEWNGSWSDRWKDWSFVDKDEKARLQHQVTEDGEFWMSYEDFIYHFTKLEICNLTADALQSDKL----QTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDDPDDSEVICSFLVALMQKNRRKDRKLGASLFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASKARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEVENTISVDRP-----VPQPGSSDQESEEQQQFRNIFKQIAGDDMEICADELKKVLNTVVNKHKDLKTHGFTLESCRSMIALMDTDGSGKLNLQEFHHLWNKIKAWQKIFKHYDTDQSGTINSYEMRNAVNDAGFHLNNQLYDIITMRYADKHMNIDFDSFICCFVRLEGMFRAFHAFDKDGDGIIKLNVLEWLQLTMY 728
BLAST of Calpain-B vs. Ensembl Human
Match: CAPN3 (calpain 3 [Source:HGNC Symbol;Acc:HGNC:1480]) HSP 1 Score: 562.762 bits (1449), Expect = 0.000e+0 Identity = 290/671 (43.22%), Postives = 425/671 (63.34%), Query Frame = 1
Query: 433 KNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEA-DPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNEN--TGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQM-KDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEI-------------------------------------VAPDWKKEIP---PEVKREEDANNVKLQNAFKVISGADGEIDADELQQILD-VSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYI 2310
K L+ DP FP + TSL+Y + P + WKRP E+ +N FI DG +R D+ QGELGDCW LAA++ L++ L+ +VIP DQ F +Y G F F+ W++G W DVV+DD LPT +LV+ S NEFWSALLEKAYAK+ GSYEALKGG+++EAMEDFTGG+ E F++ AP +++ IM K + R SLMGCSI+ P + E + GL++GHAYS+T + V K +V LVR+RNPWG EW G++SD+ +W+ + E+ RL +DGEFWM+++DF+ +FTKLEIC+L+ ++ +K W + + G W + SAGGCRNF +TF NPQ+++K+++ D++ +++ + ++ LMQK R RK G LFTIG+AIY++ K+ +K L K +F AS A+S T+IN+REV R +LP +YVIVPST+ +E +F+LR+FSE+ S++++ + +PD +K+ P P +E + +N FK I+G D EI ADEL+++L+ V K ++ K +GF+ E+CRSM+A+MDTD SG+L +EF LW+ ++ W+ +FK +D DQSGT NSYE+R A+N GF ++N+++ I RYA+K I FD +I
Sbjct: 71 KKVLYVDPEFPPDETSLFYSQKFPIQFVWKRPPEICENPRFIIDGANRTDICQGELGDCWFLAAIACLTLNQHLLFRVIPHDQSFIENYAGIFHFQFWRYGEWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVAEFFEI-RDAPSDMYKIMKKAIERGSLMGCSIDTIIPVQYETRMACGLVRGHAYSVTGLDEVPFKGEKVKLVRLRNPWGQ-VEWNGSWSDRWKDWSFVDKDEKARLQHQVTEDGEFWMSYEDFIYHFTKLEICNLTADALQSDKL----QTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDDPDDSEVICSFLVALMQKNRRKDRKLGASLFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASKARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEVENTISVDRPVKKKKTKPIIFVSDRANSNKELGVDQESEEGKGKTSPDKQKQSPQPQPGSSDQESEEQQQFRNIFKQIAGDDMEICADELKKVLNTVVNKHKDLKTHGFTLESCRSMIALMDTDGSGKLNLQEFHHLWNKIKAWQKIFKHYDTDQSGTINSYEMRNAVNDAGFHLNNQLYDIITMRYADKHMNIDFDSFI 735
BLAST of Calpain-B vs. Ensembl Human
Match: CAPN1 (calpain 1 [Source:HGNC Symbol;Acc:HGNC:1476]) HSP 1 Score: 552.747 bits (1423), Expect = 0.000e+0 Identity = 291/649 (44.84%), Postives = 425/649 (65.49%), Query Frame = 1
Query: 406 YNQKRMLKAKN-SLFEDPFFPANNTSLYYREAPPQK-----IEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEADPT-KVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKV---VDPDE-NDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSL--LDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILD-VSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYI 2310
Y Q R+ ++ +LF D FP SL Y++ P I+WKRP E+ N FI DG +R D+ QG LGDCWLLAA++SL++ L+H+V+P Q F Y G F F+LWQFG W DVVVDD LP G+LV++HS + NEFWSALLEKAYAKV GSYEAL GGS+SE EDFTGG+TE ++L AP +L+ I++K + R SL+GCSI+ +EA K L+KGHAYS+T K VN + V+L+R+RNPWG EWTGA+SD S EWN + ER +L + E DGEFWM+F+DF++ FT+LEIC+L+P++ L+++ KW + G W++ +AGGCRN+ TF +NPQFK+++ DPD+ D E+ + ++ LMQK R R+ G+D+ TIG+A+Y++ + L + +F AS A+S FINLREV R +LP G+YV+VPSTF +E F+LR FSE+ + ++D++ + PD +V EE+ + + F+ ++G D EI EL+ IL+ + K ++ + GFS E+CRSMV +MD D +G+LG EF +LW+ +R + ++F+KFD D+SG+ ++YE+R A+ GF+++ +++ IITRY+ + FD+++
Sbjct: 42 YEQLRVRCLQSGTLFRDEAFPPVPQSLGYKDLGPNSSKTYGIKWKRPTELLSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNDTLLHRVVPHGQSFQNGYAGIFHFQLWQFGEWVDVVVDDLLPIKDGKLVFVHSAEGNEFWSALLEKAYAKVNGSYEALSGGSTSEGFEDFTGGVTEWYEL-RKAPSDLYQIILKALERGSLLGCSIDISSVLDMEAITFKKLVKGHAYSVTGAKQVNYRGQVVSLIRMRNPWGE-VEWTGAWSDSSSEWNNVDPYERDQLRVKME-DGEFWMSFRDFMREFTRLEICNLTPDA-LKSRT---IRKWNTTLYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLDETDDPDDYGDRESGCSFVLALMQKHRRRERRFGRDMETIGFAVYEVPPELVGQPAVHLKRDFFLANASRARSEQFINLREVSTRFRLPPGEYVVVPSTFEPNKEGDFVLRFFSEKSAGTVELDDQIQANLPD------EQVLSEEEIDE-NFKALFRQLAGEDMEISVKELRTILNRIISKHKDLRTKGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLSIFRKFDLDKSGSMSAYEMRMAIESAGFKLNKKLYELIITRYSEPDLAVDFDNFV 676
BLAST of Calpain-B vs. Ensembl Human
Match: CAPN1 (calpain 1 [Source:HGNC Symbol;Acc:HGNC:1476]) HSP 1 Score: 552.747 bits (1423), Expect = 0.000e+0 Identity = 291/649 (44.84%), Postives = 425/649 (65.49%), Query Frame = 1
Query: 406 YNQKRMLKAKN-SLFEDPFFPANNTSLYYREAPPQK-----IEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEADPT-KVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKV---VDPDE-NDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSL--LDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILD-VSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYI 2310
Y Q R+ ++ +LF D FP SL Y++ P I+WKRP E+ N FI DG +R D+ QG LGDCWLLAA++SL++ L+H+V+P Q F Y G F F+LWQFG W DVVVDD LP G+LV++HS + NEFWSALLEKAYAKV GSYEAL GGS+SE EDFTGG+TE ++L AP +L+ I++K + R SL+GCSI+ +EA K L+KGHAYS+T K VN + V+L+R+RNPWG EWTGA+SD S EWN + ER +L + E DGEFWM+F+DF++ FT+LEIC+L+P++ L+++ KW + G W++ +AGGCRN+ TF +NPQFK+++ DPD+ D E+ + ++ LMQK R R+ G+D+ TIG+A+Y++ + L + +F AS A+S FINLREV R +LP G+YV+VPSTF +E F+LR FSE+ + ++D++ + PD +V EE+ + + F+ ++G D EI EL+ IL+ + K ++ + GFS E+CRSMV +MD D +G+LG EF +LW+ +R + ++F+KFD D+SG+ ++YE+R A+ GF+++ +++ IITRY+ + FD+++
Sbjct: 42 YEQLRVRCLQSGTLFRDEAFPPVPQSLGYKDLGPNSSKTYGIKWKRPTELLSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNDTLLHRVVPHGQSFQNGYAGIFHFQLWQFGEWVDVVVDDLLPIKDGKLVFVHSAEGNEFWSALLEKAYAKVNGSYEALSGGSTSEGFEDFTGGVTEWYEL-RKAPSDLYQIILKALERGSLLGCSIDISSVLDMEAITFKKLVKGHAYSVTGAKQVNYRGQVVSLIRMRNPWGE-VEWTGAWSDSSSEWNNVDPYERDQLRVKME-DGEFWMSFRDFMREFTRLEICNLTPDA-LKSRT---IRKWNTTLYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLDETDDPDDYGDRESGCSFVLALMQKHRRRERRFGRDMETIGFAVYEVPPELVGQPAVHLKRDFFLANASRARSEQFINLREVSTRFRLPPGEYVVVPSTFEPNKEGDFVLRFFSEKSAGTVELDDQIQANLPD------EQVLSEEEIDE-NFKALFRQLAGEDMEISVKELRTILNRIISKHKDLRTKGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLSIFRKFDLDKSGSMSAYEMRMAIESAGFKLNKKLYELIITRYSEPDLAVDFDNFV 676
BLAST of Calpain-B vs. Ensembl Celegans
Match: clp-1 (Calpain clp-1 [Source:UniProtKB/Swiss-Prot;Acc:P34308]) HSP 1 Score: 476.863 bits (1226), Expect = 1.064e-156 Identity = 235/491 (47.86%), Postives = 336/491 (68.43%), Query Frame = 1
Query: 376 KSLKESKDWFYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEADPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPE--SELENKAGIKKSK--WEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQI 1836
+ E +D++ + + L++K LFEDP F AN++SL++ + PP+++EW RP E+ + I +G SRFDV+QGELGDCWLLAA ++L++ L ++V+P DQ F +Y G F F+ WQ+G W DVV+DDRLPT G L+Y+HS NEFWSALLEKAYAK+ GSYEALKGG++SEA+ED TGG+TE DL + P NL +MM+G SL GCSIEADP EAK GL+KGHAYSIT + V+ G+ ++R+RNPWGN EW G +SD S EW + D ++ +GL F+ DGEFWM+F DF++ F K+EIC+L P+ E+ G+K + W +G W +N +AGGCRN+I TF NPQF+V++ D D +D++ T+I ++QK R+ +++G D IG+A+Y M + ++ L K++F S +S FINLRE+ GR ++P G YV+VPSTF EEA+F+LR+++ +S+++
Sbjct: 249 RPTTEPQDFYELRDQCLESK-RLFEDPQFLANDSSLFFSKRPPKRVEWLRPGEITREPQLITEGHSRFDVIQGELGDCWLLAAAANLTLKDELFYRVVPPDQSFTENYAGIFHFQFWQYGKWVDVVIDDRLPTSNGELLYMHSASNNEFWSALLEKAYAKLFGSYEALKGGTTSEALEDMTGGLTEFIDL-KNPPRNLMQMMMRGFEMGSLFGCSIEADPNVWEAKMSNGLVKGHAYSITGCRIVDGPNGQTCILRIRNPWGNEQEWNGPWSDNSREWRSVPDSVKQDMGLKFDHDGEFWMSFDDFMRNFEKMEICNLGPDVMDEVYQMTGVKAAGMVWAANTHDGAWVRNQTAGGCRNYINTFANNPQFRVQLTDSDPDDDDELCTVIFAVLQKYRRNLKQDGLDNVPIGFAVYDMNNAGNNRGRLSKQFFAANKSAMRSAAFINLREMTGRFRVPPGNYVVVPSTFEPNEEAEFMLRVYTNGFIESEEL 737
BLAST of Calpain-B vs. Ensembl Celegans
Match: clp-1 (Calpain clp-1 [Source:UniProtKB/Swiss-Prot;Acc:P34308]) HSP 1 Score: 476.863 bits (1226), Expect = 1.064e-156 Identity = 235/491 (47.86%), Postives = 336/491 (68.43%), Query Frame = 1
Query: 376 KSLKESKDWFYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEADPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPE--SELENKAGIKKSK--WEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQI 1836
+ E +D++ + + L++K LFEDP F AN++SL++ + PP+++EW RP E+ + I +G SRFDV+QGELGDCWLLAA ++L++ L ++V+P DQ F +Y G F F+ WQ+G W DVV+DDRLPT G L+Y+HS NEFWSALLEKAYAK+ GSYEALKGG++SEA+ED TGG+TE DL + P NL +MM+G SL GCSIEADP EAK GL+KGHAYSIT + V+ G+ ++R+RNPWGN EW G +SD S EW + D ++ +GL F+ DGEFWM+F DF++ F K+EIC+L P+ E+ G+K + W +G W +N +AGGCRN+I TF NPQF+V++ D D +D++ T+I ++QK R+ +++G D IG+A+Y M + ++ L K++F S +S FINLRE+ GR ++P G YV+VPSTF EEA+F+LR+++ +S+++
Sbjct: 249 RPTTEPQDFYELRDQCLESK-RLFEDPQFLANDSSLFFSKRPPKRVEWLRPGEITREPQLITEGHSRFDVIQGELGDCWLLAAAANLTLKDELFYRVVPPDQSFTENYAGIFHFQFWQYGKWVDVVIDDRLPTSNGELLYMHSASNNEFWSALLEKAYAKLFGSYEALKGGTTSEALEDMTGGLTEFIDL-KNPPRNLMQMMMRGFEMGSLFGCSIEADPNVWEAKMSNGLVKGHAYSITGCRIVDGPNGQTCILRIRNPWGNEQEWNGPWSDNSREWRSVPDSVKQDMGLKFDHDGEFWMSFDDFMRNFEKMEICNLGPDVMDEVYQMTGVKAAGMVWAANTHDGAWVRNQTAGGCRNYINTFANNPQFRVQLTDSDPDDDDELCTVIFAVLQKYRRNLKQDGLDNVPIGFAVYDMNNAGNNRGRLSKQFFAANKSAMRSAAFINLREMTGRFRVPPGNYVVVPSTFEPNEEAEFMLRVYTNGFIESEEL 737
BLAST of Calpain-B vs. Ensembl Celegans
Match: clp-1 (Calpain clp-1 [Source:UniProtKB/Swiss-Prot;Acc:P34308]) HSP 1 Score: 476.863 bits (1226), Expect = 1.064e-156 Identity = 235/491 (47.86%), Postives = 336/491 (68.43%), Query Frame = 1
Query: 376 KSLKESKDWFYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEADPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPE--SELENKAGIKKSK--WEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQI 1836
+ E +D++ + + L++K LFEDP F AN++SL++ + PP+++EW RP E+ + I +G SRFDV+QGELGDCWLLAA ++L++ L ++V+P DQ F +Y G F F+ WQ+G W DVV+DDRLPT G L+Y+HS NEFWSALLEKAYAK+ GSYEALKGG++SEA+ED TGG+TE DL + P NL +MM+G SL GCSIEADP EAK GL+KGHAYSIT + V+ G+ ++R+RNPWGN EW G +SD S EW + D ++ +GL F+ DGEFWM+F DF++ F K+EIC+L P+ E+ G+K + W +G W +N +AGGCRN+I TF NPQF+V++ D D +D++ T+I ++QK R+ +++G D IG+A+Y M + ++ L K++F S +S FINLRE+ GR ++P G YV+VPSTF EEA+F+LR+++ +S+++
Sbjct: 249 RPTTEPQDFYELRDQCLESK-RLFEDPQFLANDSSLFFSKRPPKRVEWLRPGEITREPQLITEGHSRFDVIQGELGDCWLLAAAANLTLKDELFYRVVPPDQSFTENYAGIFHFQFWQYGKWVDVVIDDRLPTSNGELLYMHSASNNEFWSALLEKAYAKLFGSYEALKGGTTSEALEDMTGGLTEFIDL-KNPPRNLMQMMMRGFEMGSLFGCSIEADPNVWEAKMSNGLVKGHAYSITGCRIVDGPNGQTCILRIRNPWGNEQEWNGPWSDNSREWRSVPDSVKQDMGLKFDHDGEFWMSFDDFMRNFEKMEICNLGPDVMDEVYQMTGVKAAGMVWAANTHDGAWVRNQTAGGCRNYINTFANNPQFRVQLTDSDPDDDDELCTVIFAVLQKYRRNLKQDGLDNVPIGFAVYDMNNAGNNRGRLSKQFFAANKSAMRSAAFINLREMTGRFRVPPGNYVVVPSTFEPNEEAEFMLRVYTNGFIESEEL 737
BLAST of Calpain-B vs. Ensembl Celegans
Match: clp-1 (Calpain clp-1 [Source:UniProtKB/Swiss-Prot;Acc:P34308]) HSP 1 Score: 470.7 bits (1210), Expect = 3.886e-154 Identity = 235/491 (47.86%), Postives = 335/491 (68.23%), Query Frame = 1
Query: 376 KSLKESKDWFYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEADPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPE--SELENKAGIKKSK--WEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQI 1836
+ E +D++ + + L++K LFEDP F AN++SL++ + PP+++EW RP E+ + I +G SRFDV+QGELGDCWLLAA ++L++ L ++V+P DQ F +Y G F F+ WQ+G W DVV+DDRLPT G L+Y+HS NEFWSALLEKAYAK+ GSYEALKGG++SEA+ED TGG+TE DL + P NL +MM+G SL GCSIEADP EAK GL+KGHAYSIT + V+ G+ ++R+RNPWGN EW G +SD S EW + D ++ +GL F+ DGEFWM+F DF++ F K+EIC+L P+ E+ G+K + W +G W +N +AGGCRN+I TF NPQF+V++ D D +D++ T+I ++QK R+ +++G D IG+A+Y +N + L K++F S +S FINLRE+ GR ++P G YV+VPSTF EEA+F+LR+++ +S+++
Sbjct: 274 RPTTEPQDFYELRDQCLESK-RLFEDPQFLANDSSLFFSKRPPKRVEWLRPGEITREPQLITEGHSRFDVIQGELGDCWLLAAAANLTLKDELFYRVVPPDQSFTENYAGIFHFQFWQYGKWVDVVIDDRLPTSNGELLYMHSASNNEFWSALLEKAYAKLFGSYEALKGGTTSEALEDMTGGLTEFIDL-KNPPRNLMQMMMRGFEMGSLFGCSIEADPNVWEAKMSNGLVKGHAYSITGCRIVDGPNGQTCILRIRNPWGNEQEWNGPWSDNSREWRSVPDSVKQDMGLKFDHDGEFWMSFDDFMRNFEKMEICNLGPDVMDEVYQMTGVKAAGMVWAANTHDGAWVRNQTAGGCRNYINTFANNPQFRVQLTDSDPDDDDELCTVIFAVLQKYRRNLKQDGLDNVPIGFAVYDAGNN---RGRLSKQFFAANKSAMRSAAFINLREMTGRFRVPPGNYVVVPSTFEPNEEAEFMLRVYTNGFIESEEL 759
BLAST of Calpain-B vs. Ensembl Celegans
Match: clp-1 (Calpain clp-1 [Source:UniProtKB/Swiss-Prot;Acc:P34308]) HSP 1 Score: 471.085 bits (1211), Expect = 4.764e-154 Identity = 235/491 (47.86%), Postives = 335/491 (68.23%), Query Frame = 1
Query: 376 KSLKESKDWFYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEADPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPE--SELENKAGIKKSK--WEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQI 1836
+ E +D++ + + L++K LFEDP F AN++SL++ + PP+++EW RP E+ + I +G SRFDV+QGELGDCWLLAA ++L++ L ++V+P DQ F +Y G F F+ WQ+G W DVV+DDRLPT G L+Y+HS NEFWSALLEKAYAK+ GSYEALKGG++SEA+ED TGG+TE DL + P NL +MM+G SL GCSIEADP EAK GL+KGHAYSIT + V+ G+ ++R+RNPWGN EW G +SD S EW + D ++ +GL F+ DGEFWM+F DF++ F K+EIC+L P+ E+ G+K + W +G W +N +AGGCRN+I TF NPQF+V++ D D +D++ T+I ++QK R+ +++G D IG+A+Y +N + L K++F S +S FINLRE+ GR ++P G YV+VPSTF EEA+F+LR+++ +S+++
Sbjct: 295 RPTTEPQDFYELRDQCLESK-RLFEDPQFLANDSSLFFSKRPPKRVEWLRPGEITREPQLITEGHSRFDVIQGELGDCWLLAAAANLTLKDELFYRVVPPDQSFTENYAGIFHFQFWQYGKWVDVVIDDRLPTSNGELLYMHSASNNEFWSALLEKAYAKLFGSYEALKGGTTSEALEDMTGGLTEFIDL-KNPPRNLMQMMMRGFEMGSLFGCSIEADPNVWEAKMSNGLVKGHAYSITGCRIVDGPNGQTCILRIRNPWGNEQEWNGPWSDNSREWRSVPDSVKQDMGLKFDHDGEFWMSFDDFMRNFEKMEICNLGPDVMDEVYQMTGVKAAGMVWAANTHDGAWVRNQTAGGCRNYINTFANNPQFRVQLTDSDPDDDDELCTVIFAVLQKYRRNLKQDGLDNVPIGFAVYDAGNN---RGRLSKQFFAANKSAMRSAAFINLREMTGRFRVPPGNYVVVPSTFEPNEEAEFMLRVYTNGFIESEEL 780
BLAST of Calpain-B vs. Ensembl Fly
Match: CalpB (gene:FBgn0025866 transcript:FBtr0076343) HSP 1 Score: 649.818 bits (1675), Expect = 0.000e+0 Identity = 322/687 (46.87%), Postives = 455/687 (66.23%), Query Frame = 1
Query: 391 SKDWFYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEADPTKVEAKAEKGLIKGHAYSITDVK----SVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQID------EKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILDVSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYIXXXXXXXXXXXXXKAQQKTSKGEAIFPQDEFMQCVLY 2421
SK F + + A ++FEDP FPA N SL Y P + EW RP ++ + F +G SRFDV QGELGDCWLLAA ++L+ L +VIP DQDF +Y G F F+ WQ+G W +VV+DDRLPT G L+Y+HS + NEFWSALLEKAYAK+ GSYEALKGG++ EAMEDFTGG+TE +D+ APPNL+ IMMK R S+MGCS+E DP +EA+ +GLI+GHAYSIT V S +++G++ ++R+RNPWGN EW+G +SD SPEW I + ++ +GL F+ DGEFWM+FQDF+ +F ++EIC+LSP+S E++ + KWEM + GEW V+AGGCRNF+ETF NPQ+ + + DPD+ D++ T I+ LMQK RS+R G D TIG+AIY + D +F+ AS A+SP FIN REVC R KLP G Y+IVPSTF+ EE +F++R+FSE + ++ D E + +AP PP K E+D + L+ F ++G+D E+D EL++ILD S + +GFS++ RSMVAM+D D+SGRLGFEEF+ L D+ W+ VFK +D ++G+ + + +R ALN G+ ++NR+ A+ RY +++G I FDD+++ +++RT F+ + + A F D++++ +Y
Sbjct: 242 SKGDFQSLRDSCLANGTMFEDPDFPATNASLMYSRRPDRYYEWLRPGDIADDPQFFVEGYSRFDVQQGELGDCWLLAAAANLTQDSTLFFRVIPPDQDFQENYAGIFHFKFWQYGKWVEVVIDDRLPTYNGELIYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGTTCEAMEDFTGGVTEWYDI-KEAPPNLFSIMMKAAERGSMMGCSLEPDPHVLEAETPQGLIRGHAYSITKVCLMDISTPNRQGKLPMIRMRNPWGNDAEWSGPWSDSSPEWRFIPEHTKEEIGLNFDRDGEFWMSFQDFLNHFDRVEICNLSPDSLTEDQQHSSRRKWEMSMFEGEWTSGVTAGGCRNFLETFWHNPQYIISLEDPDDEDDDGKCTAIVALMQKNRRSKRNVGIDCLTIGFAIYHLTDRDMQVKPQGLNFFKYRASVARSPHFINTREVCARFKLPPGHYLIVPSTFDPNEEGEFIIRVFSETRNNMEENDDEVGFGETDDRIAPSLP---PPTPKEEDDPQRIALRRLFDSVAGSDEEVDWQELKRILDHSMRDVMVGSDGFSKDAVRSMVAMLDKDRSGRLGFEEFEALLTDIAKWRAVFKLYDTRRTGSIDGFHLRGALNSAGYHLNNRLLNALAHRYGSREGQIPFDDFLMCAIKVRTFIEMFRERDTDNSDTAFFNLDDWLERTIY 924
BLAST of Calpain-B vs. Ensembl Fly
Match: CalpB (gene:FBgn0025866 transcript:FBtr0304675) HSP 1 Score: 649.818 bits (1675), Expect = 0.000e+0 Identity = 322/687 (46.87%), Postives = 455/687 (66.23%), Query Frame = 1
Query: 391 SKDWFYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEADPTKVEAKAEKGLIKGHAYSITDVK----SVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQID------EKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILDVSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYIXXXXXXXXXXXXXKAQQKTSKGEAIFPQDEFMQCVLY 2421
SK F + + A ++FEDP FPA N SL Y P + EW RP ++ + F +G SRFDV QGELGDCWLLAA ++L+ L +VIP DQDF +Y G F F+ WQ+G W +VV+DDRLPT G L+Y+HS + NEFWSALLEKAYAK+ GSYEALKGG++ EAMEDFTGG+TE +D+ APPNL+ IMMK R S+MGCS+E DP +EA+ +GLI+GHAYSIT V S +++G++ ++R+RNPWGN EW+G +SD SPEW I + ++ +GL F+ DGEFWM+FQDF+ +F ++EIC+LSP+S E++ + KWEM + GEW V+AGGCRNF+ETF NPQ+ + + DPD+ D++ T I+ LMQK RS+R G D TIG+AIY + D +F+ AS A+SP FIN REVC R KLP G Y+IVPSTF+ EE +F++R+FSE + ++ D E + +AP PP K E+D + L+ F ++G+D E+D EL++ILD S + +GFS++ RSMVAM+D D+SGRLGFEEF+ L D+ W+ VFK +D ++G+ + + +R ALN G+ ++NR+ A+ RY +++G I FDD+++ +++RT F+ + + A F D++++ +Y
Sbjct: 242 SKGDFQSLRDSCLANGTMFEDPDFPATNASLMYSRRPDRYYEWLRPGDIADDPQFFVEGYSRFDVQQGELGDCWLLAAAANLTQDSTLFFRVIPPDQDFQENYAGIFHFKFWQYGKWVEVVIDDRLPTYNGELIYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGTTCEAMEDFTGGVTEWYDI-KEAPPNLFSIMMKAAERGSMMGCSLEPDPHVLEAETPQGLIRGHAYSITKVCLMDISTPNRQGKLPMIRMRNPWGNDAEWSGPWSDSSPEWRFIPEHTKEEIGLNFDRDGEFWMSFQDFLNHFDRVEICNLSPDSLTEDQQHSSRRKWEMSMFEGEWTSGVTAGGCRNFLETFWHNPQYIISLEDPDDEDDDGKCTAIVALMQKNRRSKRNVGIDCLTIGFAIYHLTDRDMQVKPQGLNFFKYRASVARSPHFINTREVCARFKLPPGHYLIVPSTFDPNEEGEFIIRVFSETRNNMEENDDEVGFGETDDRIAPSLP---PPTPKEEDDPQRIALRRLFDSVAGSDEEVDWQELKRILDHSMRDVMVGSDGFSKDAVRSMVAMLDKDRSGRLGFEEFEALLTDIAKWRAVFKLYDTRRTGSIDGFHLRGALNSAGYHLNNRLLNALAHRYGSREGQIPFDDFLMCAIKVRTFIEMFRERDTDNSDTAFFNLDDWLERTIY 924
BLAST of Calpain-B vs. Ensembl Fly
Match: CalpA (gene:FBgn0012051 transcript:FBtr0340532) HSP 1 Score: 563.533 bits (1451), Expect = 0.000e+0 Identity = 288/614 (46.91%), Postives = 393/614 (64.01%), Query Frame = 1
Query: 313 LSPDPKQTDSESIYGSRFHVSKSL---KESKDWFYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEADPTKVEAKAEKGLIKGHAYSITDVKSVN----SKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDE------KTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILDVSFKRENFK---FNGFSRETC-RSMVAMMDTDQSGRLGF 2103
+ P Q++S YG+ S SL E +D+ L A SLFEDP FPA+N SL + P + IEW RP E+ +N F +G SRFDV QGELGDCWLLAA ++L+ L +VIP++Q F+ +Y G F FR WQ+G W DV++DDRLPT G L+Y+HS + NEFWSALLEKAYAK+ GSYEALKGGS+ EAMEDFTGG++E +DL AP NL+ I+ K R S+MGCSIE DP EA+ +GLI+GHAYSIT V ++ +++G++ ++R+RNPWGN EW G +SD SPEW I + ++ +GLTF+ DGEFWM+FQDF+ +F ++EIC+LSP+S E++ K KWEM + GEW V+AGGCRNF++TF NPQ+ + +VDPDE D E T+I+ LMQK RS+R G + TIG+AIY + D + +F+ +S +SP FIN REVC R KLP G Y+IVPSTF+ EE +F++R+FSE + ++ D+ K + + P + P + ++E L+ F I+G D E+D EL++ILD S + + K FN FS + A D +G G
Sbjct: 58 VQPLQSQSNSTLYYGNLGEKSSSLGPYSEVQDYETILNSCL-ASGSLFEDPLFPASNESLQFSRRPDRHIEWLRPHEIAENPQFFVEGYSRFDVQQGELGDCWLLAATANLTQESNLFFRVIPAEQSFEENYAGIFHFRFWQYGKWVDVIIDDRLPTYNGELMYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGSTCEAMEDFTGGVSEWYDL-KEAPGNLFTILQKAAERNSMMGCSIEPDPNVTEAETPQGLIRGHAYSITKVCLIDIVTPNRQGKIPMIRMRNPWGNEAEWNGPWSDSSPEWRYIPEEQKAEIGLTFDRDGEFWMSFQDFLNHFDRVEICNLSPDSLTEDQQNSGKRKWEMSMYEGEWTPGVTAGGCRNFLDTFWHNPQYIITLVDPDEEDEEGQCTVIVALMQKNRRSKRNMGMECLTIGFAIYSLNDRELENRPQGLNFFRYKSSVGRSPHFINTREVCARFKLPPGHYLIVPSTFDPNEEGEFIIRVFSETQNNMEENDDHVGYGGKADTITPGFPTPKPIDPQKE------GLRRLFDSIAGKDMEVDWMELKRILDHSMRDDLPKPVVFNRFSNNMAFETQAAGPGDDGAGACGL 663 HSP 2 Score: 107.071 bits (266), Expect = 2.156e-23 Identity = 51/131 (38.93%), Postives = 86/131 (65.65%), Query Frame = 1
Query: 2029 NGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYIXXXXXXXXXXXXXKAQQKTSKGEAIFPQDEFMQCVLY 2421
+GFS++ CRSMVAM+D D+SG+LGFEEF+ L ++ WK +FK +D + +G + +++R+ALN G+ ++NRV + RY ++ G I FDD+I+ V+++T FK + A F +E+++ +Y
Sbjct: 712 HGFSKDVCRSMVAMLDADKSGKLGFEEFETLLSEIAKWKAIFKVYDVENTGRVSGFQLREALNSAGYHLNNRVLNVLGHRYGSRDGKIAFDDFIMCAVKIKTYIDIFKERDTEKNETATFTLEEWIERTIY 842
BLAST of Calpain-B vs. Ensembl Fly
Match: CalpA (gene:FBgn0012051 transcript:FBtr0112869) HSP 1 Score: 561.222 bits (1445), Expect = 0.000e+0 Identity = 277/572 (48.43%), Postives = 376/572 (65.73%), Query Frame = 1
Query: 430 AKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEADPTKVEAKAEKGLIKGHAYSITDVKSVN----SKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDE------KTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILDVSFKRENFK---FNGFSRETC-RSMVAMMDTDQSGRLGF 2103
A SLFEDP FPA+N SL + P + IEW RP E+ +N F +G SRFDV QGELGDCWLLAA ++L+ L +VIP++Q F+ +Y G F FR WQ+G W DV++DDRLPT G L+Y+HS + NEFWSALLEKAYAK+ GSYEALKGGS+ EAMEDFTGG++E +DL AP NL+ I+ K R S+MGCSIE DP EA+ +GLI+GHAYSIT V ++ +++G++ ++R+RNPWGN EW G +SD SPEW I + ++ +GLTF+ DGEFWM+FQDF+ +F ++EIC+LSP+S E++ K KWEM + GEW V+AGGCRNF++TF NPQ+ + +VDPDE D E T+I+ LMQK RS+R G + TIG+AIY + D + +F+ +S +SP FIN REVC R KLP G Y+IVPSTF+ EE +F++R+FSE + ++ D+ K + + P + P + ++E L+ F I+G D E+D EL++ILD S + + K FN FS + A D +G G
Sbjct: 84 ASGSLFEDPLFPASNESLQFSRRPDRHIEWLRPHEIAENPQFFVEGYSRFDVQQGELGDCWLLAATANLTQESNLFFRVIPAEQSFEENYAGIFHFRFWQYGKWVDVIIDDRLPTYNGELMYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGSTCEAMEDFTGGVSEWYDL-KEAPGNLFTILQKAAERNSMMGCSIEPDPNVTEAETPQGLIRGHAYSITKVCLIDIVTPNRQGKIPMIRMRNPWGNEAEWNGPWSDSSPEWRYIPEEQKAEIGLTFDRDGEFWMSFQDFLNHFDRVEICNLSPDSLTEDQQNSGKRKWEMSMYEGEWTPGVTAGGCRNFLDTFWHNPQYIITLVDPDEEDEEGQCTVIVALMQKNRRSKRNMGMECLTIGFAIYSLNDRELENRPQGLNFFRYKSSVGRSPHFINTREVCARFKLPPGHYLIVPSTFDPNEEGEFIIRVFSETQNNMEENDDHVGYGGKADTITPGFPTPKPIDPQKE------GLRRLFDSIAGKDMEVDWMELKRILDHSMRDDLPKPVVFNRFSNNMAFETQAAGPGDDGAGACGL 648 HSP 2 Score: 107.457 bits (267), Expect = 1.936e-23 Identity = 51/131 (38.93%), Postives = 86/131 (65.65%), Query Frame = 1
Query: 2029 NGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYIXXXXXXXXXXXXXKAQQKTSKGEAIFPQDEFMQCVLY 2421
+GFS++ CRSMVAM+D D+SG+LGFEEF+ L ++ WK +FK +D + +G + +++R+ALN G+ ++NRV + RY ++ G I FDD+I+ V+++T FK + A F +E+++ +Y
Sbjct: 697 HGFSKDVCRSMVAMLDADKSGKLGFEEFETLLSEIAKWKAIFKVYDVENTGRVSGFQLREALNSAGYHLNNRVLNVLGHRYGSRDGKIAFDDFIMCAVKIKTYIDIFKERDTEKNETATFTLEEWIERTIY 827
BLAST of Calpain-B vs. Ensembl Fly
Match: CalpA (gene:FBgn0012051 transcript:FBtr0086529) HSP 1 Score: 561.222 bits (1445), Expect = 0.000e+0 Identity = 277/572 (48.43%), Postives = 376/572 (65.73%), Query Frame = 1
Query: 430 AKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEADPTKVEAKAEKGLIKGHAYSITDVKSVN----SKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDE------KTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILDVSFKRENFK---FNGFSRETC-RSMVAMMDTDQSGRLGF 2103
A SLFEDP FPA+N SL + P + IEW RP E+ +N F +G SRFDV QGELGDCWLLAA ++L+ L +VIP++Q F+ +Y G F FR WQ+G W DV++DDRLPT G L+Y+HS + NEFWSALLEKAYAK+ GSYEALKGGS+ EAMEDFTGG++E +DL AP NL+ I+ K R S+MGCSIE DP EA+ +GLI+GHAYSIT V ++ +++G++ ++R+RNPWGN EW G +SD SPEW I + ++ +GLTF+ DGEFWM+FQDF+ +F ++EIC+LSP+S E++ K KWEM + GEW V+AGGCRNF++TF NPQ+ + +VDPDE D E T+I+ LMQK RS+R G + TIG+AIY + D + +F+ +S +SP FIN REVC R KLP G Y+IVPSTF+ EE +F++R+FSE + ++ D+ K + + P + P + ++E L+ F I+G D E+D EL++ILD S + + K FN FS + A D +G G
Sbjct: 84 ASGSLFEDPLFPASNESLQFSRRPDRHIEWLRPHEIAENPQFFVEGYSRFDVQQGELGDCWLLAATANLTQESNLFFRVIPAEQSFEENYAGIFHFRFWQYGKWVDVIIDDRLPTYNGELMYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGSTCEAMEDFTGGVSEWYDL-KEAPGNLFTILQKAAERNSMMGCSIEPDPNVTEAETPQGLIRGHAYSITKVCLIDIVTPNRQGKIPMIRMRNPWGNEAEWNGPWSDSSPEWRYIPEEQKAEIGLTFDRDGEFWMSFQDFLNHFDRVEICNLSPDSLTEDQQNSGKRKWEMSMYEGEWTPGVTAGGCRNFLDTFWHNPQYIITLVDPDEEDEEGQCTVIVALMQKNRRSKRNMGMECLTIGFAIYSLNDRELENRPQGLNFFRYKSSVGRSPHFINTREVCARFKLPPGHYLIVPSTFDPNEEGEFIIRVFSETQNNMEENDDHVGYGGKADTITPGFPTPKPIDPQKE------GLRRLFDSIAGKDMEVDWMELKRILDHSMRDDLPKPVVFNRFSNNMAFETQAAGPGDDGAGACGL 648 HSP 2 Score: 107.457 bits (267), Expect = 1.936e-23 Identity = 51/131 (38.93%), Postives = 86/131 (65.65%), Query Frame = 1
Query: 2029 NGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYIXXXXXXXXXXXXXKAQQKTSKGEAIFPQDEFMQCVLY 2421
+GFS++ CRSMVAM+D D+SG+LGFEEF+ L ++ WK +FK +D + +G + +++R+ALN G+ ++NRV + RY ++ G I FDD+I+ V+++T FK + A F +E+++ +Y
Sbjct: 697 HGFSKDVCRSMVAMLDADKSGKLGFEEFETLLSEIAKWKAIFKVYDVENTGRVSGFQLREALNSAGYHLNNRVLNVLGHRYGSRDGKIAFDDFIMCAVKIKTYIDIFKERDTEKNETATFTLEEWIERTIY 827
BLAST of Calpain-B vs. Ensembl Zebrafish
Match: zgc:55262 (zgc:55262 [Source:ZFIN;Acc:ZDB-GENE-040426-2814]) HSP 1 Score: 586.645 bits (1511), Expect = 0.000e+0 Identity = 294/632 (46.52%), Postives = 419/632 (66.30%), Query Frame = 1
Query: 442 LFEDPFFPANNTSLYYREAPP-----QKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEADPT-KVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKS--LLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILD-VSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYI 2310
LFEDP F A+++SL + E P Q + W RPKEM FI DG +R D+ QG LGDCWLLAA++SL++ L+H+V+P QDFD Y G F F+ WQFG W DVV+DDRLPT G+L+++HS + EFWSALLEKAYAK+ G YEAL GGS+ E EDFTGG+TEM++L AP +L+ I+ + + R SL+GCSI+ +EA K L+KGHAYS+T + V + LVR+RNPWG EWTGA+SD S EW+ + R RL +DGEFWM+F DF++ FT+LEIC+L+ ++ ++ KW NGEW++ +AGGCRN+ TF +NPQFKV + PD + ++ + ++ LMQK+ R +R+EGQD+ TIG+AIY++ +S L + +F AS A+S FINLREV R +LP G+Y+IVPSTF +EA F+LR+FSE+P +S+++D+K EIP E + +E + ++ F+ ++GAD EI ELQ IL+ + K ++ K +GF +E+CRSM+ +MDTD SG+LG EF VLW+ ++ + +F+ D D+SGT +SYE+R+AL GF+++N +F II RY + + FD+++
Sbjct: 50 LFEDPMFTASSSSLGFNELGPRSSKTQGVRWMRPKEMCARPQFIVDGATRTDICQGALGDCWLLAAIASLTLNDNLLHRVVPHGQDFDSRYAGIFHFQFWQFGEWVDVVIDDRLPTKDGKLLFVHSAEGGEFWSALLEKAYAKLNGCYEALSGGSTCEGFEDFTGGVTEMYEL-KKAPADLFSIIGRAIERGSLLGCSIDITSKFDMEAVTFKKLVKGHAYSVTGAEEVVYRGNMTKLVRIRNPWGE-VEWTGAWSDNSREWDNVDRSVRGRLQ-NRSEDGEFWMSFSDFLREFTRLEICNLTADALQASQV----KKWSTANYNGEWRRGSTAGGCRNYPATFWINPQFKVALKHPD-SPGQSECSFLVALMQKDRRKKRREGQDMETIGFAIYEVPREFLGQSGVHLKRDFFLTHASSARSELFINLREVSSRFRLPAGEYIIVPSTFEPNKEADFVLRVFSEKPANSEEMDDKV-------MAEIPEEQRLDESQIDAGFKSLFRQLAGADMEISVTELQTILNRIIAKHKDLKTDGFGKESCRSMINLMDTDGSGKLGLVEFHVLWEKIKRYLQIFRDHDVDKSGTMSSYEMRKALETAGFKLNNHLFQLIILRYTEEDLSVDFDNFV 666
BLAST of Calpain-B vs. Ensembl Zebrafish
Match: capn9 (calpain 9 [Source:ZFIN;Acc:ZDB-GENE-010724-2]) HSP 1 Score: 577.785 bits (1488), Expect = 0.000e+0 Identity = 310/666 (46.55%), Postives = 432/666 (64.86%), Query Frame = 1
Query: 415 KRMLKAKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIE-ADPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQIL-DVSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYIXXXXXXXXXXXXXKAQQKTSKGEAIFPQDEFM 2406
+R K LFEDP FPA + SL+Y + P EWKRP E+ + FI G +R D+ QG+LGDCWLLAA++SL++ +++V+P+DQ FDR Y G F F+ WQ W DVVVDDRLP VR +LV+LHS D NEFWSALLEKAYAK+ GSYEALKGGS+ EAMEDFTGG+ EM++ + P NL+ I+ K + R S++GCSI+ + EA+ GL+KGHAYS+T V+ VN + +V L+RVRNPWG EW G +SD S EW I E+KRL DDGEFWM F DF + K+EIC+LSP++ ++ + +W + + G W + +AGGCRN+I+TF NPQFK+ + +PD D + ++I+ LMQK R RKEG DL TIG+AIY+ D D+ K +F+ S A+S T+IN+REV R +LP G Y++VP+TF +EA FL+R+FSE+ + ++ K E P+ K PPE + +E+ L+ F+ ++G D I A ELQ +L +V +R+ KF+G S TC S++ +MD D SG + F EFKV WD L+ W +F +D D+SGT +SYE+R ALN G +++NR+ + R+A+++ I FDDY+ +VRL +F F+A KGE +F+
Sbjct: 31 RRECLEKGKLFEDPDFPAVDGSLFYSQRVPVNFEWKRPGEICKEPQFITGGATRTDICQGQLGDCWLLAAIASLTLKEETLNRVVPNDQTFDRGYAGIFHFQFWQHNKWMDVVVDDRLPCVRDKLVFLHSADNNEFWSALLEKAYAKLNGSYEALKGGSTMEAMEDFTGGVGEMYET-KNGPNNLFLILKKAIERGSMLGCSIDITSSAESEAQTSTGLVKGHAYSVTGVEEVNYRGAKVQLIRVRNPWG-QVEWNGPWSDNSREWTVIDSSEKKRLLQNSLDDGEFWMEFGDFKSNYDKVEICNLSPDALSDDVS----KQWAVNLFEGSWIRGSTAGGCRNYIDTFWTNPQFKLHLENPD--DGDKLCSVIVALMQKNRRRLRKEGLDLETIGFAIYEAPD---DQDHQGKDFFRYNPSKARSRTYINVREVSERFRLPPGNYLLVPTTFQPHKEADFLIRLFSEKKAGAIEVGNKIEADLPEPPKPNPPEEETDEEKG---LRKLFEQVAGPDNVISARELQHVLNNVLGRRKEVKFDGLSLNTCISIINLMDVDGSGMMEFSEFKVFWDKLKKWIMLFLSYDVDRSGTMSSYELRSALNAAGMKLNNRILQLLGLRFADEKLEIDFDDYLTCIVRLENMFRIFQALDAQKKGEVSLNMHQFL 682
BLAST of Calpain-B vs. Ensembl Zebrafish
Match: capn3b (calpain 3b [Source:ZFIN;Acc:ZDB-GENE-041001-149]) HSP 1 Score: 564.303 bits (1453), Expect = 0.000e+0 Identity = 305/671 (45.45%), Postives = 436/671 (64.98%), Query Frame = 1
Query: 433 KNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEA-DPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGR---VNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTE---IVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILD-VSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYIXXXXXXXXXXXXXKAQQKTSKGEAIFPQDEFMQCVLY 2421
K LFEDP FPA ++SL+Y E P K+EWKRP E+ +N FI G +R D+ QG+LGDCWLLAA++ L++ ++ +V+P DQ F +Y G F F+ W++G W DVV+DDRLPT + +LV+ S NEFWSAL+EKAYAK+ GSYEALKGG+S EAMEDFTGG+TE +++ + AP L++IM K + R SLMGC+I+ PT K GL++GHAYS+T V+ K G+ + LVRVR+PWG + + KS +W E++ E+ + L + GEFWM F++F K FTKLEIC+L+P++ LE+ + KW + + G W K SAGGCRNF +T+ NPQF++ +++ D E T T+++ LMQK R R G L IG+AIY+MK N + L K +F AS A+S +++N+REV R L G+YVI+PSTF+ +E++FLLR+FSE SK ID + E + + KK+I P + EE + + F+ I+G D EI+A+EL+ +L+ V K + K GFS E+CRSM+A++DTD +G L +EFK LW+ ++ WK VF +FD D+S T +S+E+R AL GF+++N+++ I RYAN+ + FD YI LVRL +F F+A K G E++Q +Y
Sbjct: 62 KKVLFEDPLFPAQDSSLFYSEKFPLKLEWKRPMEICKNPQFILGGANRTDICQGDLGDCWLLAAIACLTLNDVILKRVVPHDQSFTENYAGIFHFQFWRYGEWVDVVIDDRLPTYKNKLVFTRSGQNNEFWSALMEKAYAKLHGSYEALKGGNSLEAMEDFTGGVTEFYEI-TEAPKELYNIMRKALKRGSLMGCAIDTLVPTAQRTKTASGLVRGHAYSVTGVEQGKRKDGKDLMIRLVRVRDPWGVA----PPPACKSNDWVELAKTEQDKQRLQPGEQGEFWMCFEEFQKNFTKLEICNLTPDT-LEDD---QMFKWNVTVHEGRWVKGCSAGGCRNFPDTYWTNPQFRLVLLEADA--KEKTCTVVVALMQKGRRVERCSGATLHNIGFAIYEMKGNQQQQ--LSKDFFLYNASTARSKSYVNIREVTERFCLKPGEYVIIPSTFDPHKESEFLLRVFSENRSTSKVIDAEIEAEPMQVDNVKKKIKP-FEEEETEEEKQFRAIFQQIAGDDMEINANELRTVLNRVVAKHKELKTEGFSLESCRSMIALLDTDGTGHLNLQEFKHLWNKIKQWKLVFTRFDTDKSSTISSFEMRNALTEAGFQLNNQLYDIICMRYANEHMELDFDSYISCLVRLEGMFRAFRAFDKDGDGLIKLNVFEWLQLTMY 718
BLAST of Calpain-B vs. Ensembl Zebrafish
Match: si:dkeyp-50d11.2 (si:dkeyp-50d11.2 [Source:ZFIN;Acc:ZDB-GENE-091113-22]) HSP 1 Score: 559.296 bits (1440), Expect = 0.000e+0 Identity = 289/642 (45.02%), Postives = 418/642 (65.11%), Query Frame = 1
Query: 412 QKRMLKAKNSLFEDPFFPANNTSLYYREAPP-----QKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEADP-TKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDK--SLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILD-VSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYI 2310
+ R L++ + LFED FPA +SL ++E P + + W RP E + FI DG +R D+ QG LGDCWLLAA++ L++ L+ +V+P Q F + Y G F F+ WQFG W DVV+DDRLP G+L+++HS + +EFWSAL+EKAYAK+ G YEAL GGS+SE EDFTGG+TEM++L +APPNL+ I+ + V R SLMGCSI+ +EA K L+KGHAYSIT V V +R + L+R+RNPWG EWTG +SD+S EW EI R RL ++DGEFWM F DF + F++LEIC+L+ ++ L+++ + KW + GEW++ +AGGCRN+ TF +NPQFK+ + +PD DN+ T ++ LMQK R R+EG+D+ TIG+AIY++ + L + +F S A+S FINLREV R LP G+Y+IVPSTF +E F+LR+FSE+ DS+++D+ EI A ++P E +E + + F ++G + EI+ +L+ IL+ V K ++ K +GF +ETCR M+ +MDT +G+LG +F VLW+ + + VF++FD D+SGT +SYE+R AL GF+++N +F II RYA + FD+++
Sbjct: 41 RTRCLQSGH-LFEDDIFPAQQSSLGFKELGPNSSKTRGVRWMRPTEFCSDPHFIVDGATRTDICQGALGDCWLLAAIACLTLNEPLLRRVVPHGQSFHQQYAGIFHFQFWQFGDWVDVVIDDRLPVRDGKLLFVHSAEGSEFWSALVEKAYAKLNGCYEALSGGSTSEGFEDFTGGVTEMYEL-KNAPPNLFSIITRAVERGSLMGCSIDITSFFDMEAITFKKLVKGHAYSITGVDEVEYRRSQTKLLRIRNPWGE-VEWTGPWSDESKEWREIDASVRSRLH-NCQEDGEFWMAFSDFKREFSRLEICNLTADA-LQSR---EVKKWNSSLYPGEWRRGSTAGGCRNYPATFWINPQFKIVLKEPD-TDNQEGCTFLVALMQKNRRQLRREGKDMETIGFAIYEVPKEYLGRVGVHLKRDFFLKHTSSARSELFINLREVSSRFSLPAGEYIIVPSTFEPQKEGDFVLRVFSEKATDSQELDD--EISA-----DVPEEPVLQESDIDEGFKALFAKLAGPEMEINVAKLEMILNRVVSKHKDIKTDGFGKETCRGMINLMDTTGTGKLGLTDFHVLWEKFKRYLAVFREFDIDKSGTMSSYEMRLALESAGFKLTNNLFQLIILRYAKSDLNVDFDNFV 666
BLAST of Calpain-B vs. Ensembl Zebrafish
Match: capn3b (calpain 3b [Source:ZFIN;Acc:ZDB-GENE-041001-149]) HSP 1 Score: 558.14 bits (1437), Expect = 0.000e+0 Identity = 305/675 (45.19%), Postives = 436/675 (64.59%), Query Frame = 1
Query: 433 KNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEA-DPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGR---VNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIY----QMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTE---IVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILD-VSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYIXXXXXXXXXXXXXKAQQKTSKGEAIFPQDEFMQCVLY 2421
K LFEDP FPA ++SL+Y E P K+EWKRP E+ +N FI G +R D+ QG+LGDCWLLAA++ L++ ++ +V+P DQ F +Y G F F+ W++G W DVV+DDRLPT + +LV+ S NEFWSAL+EKAYAK+ GSYEALKGG+S EAMEDFTGG+TE +++ + AP L++IM K + R SLMGC+I+ PT K GL++GHAYS+T V+ K G+ + LVRVR+PWG + + KS +W E++ E+ + L + GEFWM F++F K FTKLEIC+L+P++ LE+ + KW + + G W K SAGGCRNF +T+ NPQF++ +++ D E T T+++ LMQK R R G L IG+AIY +MK N + L K +F AS A+S +++N+REV R L G+YVI+PSTF+ +E++FLLR+FSE SK ID + E + + KK+I P + EE + + F+ I+G D EI+A+EL+ +L+ V K + K GFS E+CRSM+A++DTD +G L +EFK LW+ ++ WK VF +FD D+S T +S+E+R AL GF+++N+++ I RYAN+ + FD YI LVRL +F F+A K G E++Q +Y
Sbjct: 62 KKVLFEDPLFPAQDSSLFYSEKFPLKLEWKRPMEICKNPQFILGGANRTDICQGDLGDCWLLAAIACLTLNDVILKRVVPHDQSFTENYAGIFHFQFWRYGEWVDVVIDDRLPTYKNKLVFTRSGQNNEFWSALMEKAYAKLHGSYEALKGGNSLEAMEDFTGGVTEFYEI-TEAPKELYNIMRKALKRGSLMGCAIDTLVPTAQRTKTASGLVRGHAYSVTGVEQGKRKDGKDLMIRLVRVRDPWGVA----PPPACKSNDWVELAKTEQDKQRLQPGEQGEFWMCFEEFQKNFTKLEICNLTPDT-LEDD---QMFKWNVTVHEGRWVKGCSAGGCRNFPDTYWTNPQFRLVLLEADA--KEKTCTVVVALMQKGRRVERCSGATLHNIGFAIYEVPKEMKGNQQQQ--LSKDFFLYNASTARSKSYVNIREVTERFCLKPGEYVIIPSTFDPHKESEFLLRVFSENRSTSKVIDAEIEAEPMQVDNVKKKIKP-FEEEETEEEKQFRAIFQQIAGDDMEINANELRTVLNRVVAKHKELKTEGFSLESCRSMIALLDTDGTGHLNLQEFKHLWNKIKQWKLVFTRFDTDKSSTISSFEMRNALTEAGFQLNNQLYDIICMRYANEHMELDFDSYISCLVRLEGMFRAFRAFDKDGDGLIKLNVFEWLQLTMY 722
BLAST of Calpain-B vs. Ensembl Xenopus
Match: tgfbrap1 (transforming growth factor beta receptor associated protein 1 [Source:Xenbase;Acc:XB-GENE-1009020]) HSP 1 Score: 614.764 bits (1584), Expect = 0.000e+0 Identity = 321/665 (48.27%), Postives = 433/665 (65.11%), Query Frame = 1
Query: 433 KNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIE-ADPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILDVSFKR-ENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYIXXXXXXXXXXXXXKAQQKTSKGEAI-FPQDEFMQCVL 2418
K LFED FPAN++SL+Y E P WKRPKEM + FI G SR DV QG+LGDCWLLAA++SL++ ++++V+P DQ F Y G F F+ WQ W DVV+DDRLPT R RLV++HS DLNEFWSALLEKAYAK+ GSYEALKGGS+ EAMEDFTGG+TE +++ SAP NL+DI+ K + + S++GCSI+ + EAK +GL+KGHAYSIT VN K +V L+R+RNPWG EW GA+SD S EWN I E+ RL DDGEFWM+F+DF ++F KLEIC+L+P+S L++ A K KWE+ + G W + +AGGCRNF+ETF NPQ K+ + + D D + T I LMQK R RK G DL TIGY IYQ + +S+L K +F+ S A+S T++NLRE+ R KLP G Y+ +P+TF +EA F LRIFSE+ D K++D + I D P +E + + F+ ISG D E+ A+ELQ +L+ +R +N KF S +C +++++MD +G+LGF+EFK+ WD L+ W +F ++D D+SGT +SYE+R AL GF ++N V I+ RYA+ Q I FDD++ LVRL + FK ++ E I Q EF+ +
Sbjct: 39 KGVLFEDVDFPANDSSLFYSEKPSVPFVWKRPKEMVADPEFILGGASRTDVCQGDLGDCWLLAAIASLTLNDKILYRVVPPDQSFSTGYAGIFHFQFWQHNEWLDVVIDDRLPTFRNRLVFVHSADLNEFWSALLEKAYAKLNGSYEALKGGSTLEAMEDFTGGVTETYEI-RSAPANLFDILDKAIKKGSMLGCSIDITSSAETEAKTPQGLVKGHAYSITGADVVNFKGQKVKLIRIRNPWGQ-VEWNGAWSDNSSEWNIIGAAEKNRLSQASLDDGEFWMDFEDFKRHFDKLEICNLTPDS-LDDDA---KHKWEVTVHEGSWIRGSTAGGCRNFLETFWSNPQIKLSLTEQD--DGQKECTFIAALMQKNRRKLRKTGADLLTIGYTIYQCPGS---ESILSKDFFKFNPSKARSKTYVNLREISQRFKLPPGDYMFIPTTFEPHQEADFCLRIFSEKKADIKEMDGEVNI---DLPDPPAPTPPHKETEEERQFRTLFEQISGQDLEVSAEELQYVLNAVLRRTKNIKFKEMSLLSCNNIISLMDASGNGKLGFDEFKIFWDKLKTWIGIFMQYDLDKSGTMSSYELRLALKSAGFHLNNIVLQLIVLRYADDQFQIEFDDFLNCLVRLENSYRVFKQLNRSDTTETIQLNQAEFIALTM 689
BLAST of Calpain-B vs. Ensembl Xenopus
Match: tgfbrap1 (transforming growth factor beta receptor associated protein 1 [Source:Xenbase;Acc:XB-GENE-1009020]) HSP 1 Score: 612.838 bits (1579), Expect = 0.000e+0 Identity = 310/628 (49.36%), Postives = 417/628 (66.40%), Query Frame = 1
Query: 433 KNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIE-ADPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILDVSFKR-ENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYI 2310
K LFED FPAN++SL+Y E P WKRPKEM + FI G SR DV QG+LGDCWLLAA++SL++ ++++V+P DQ F Y G F F+ WQ W DVV+DDRLPT R RLV++HS DLNEFWSALLEKAYAK+ GSYEALKGGS+ EAMEDFTGG+TE +++ SAP NL+DI+ K + + S++GCSI+ + EAK +GL+KGHAYSIT VN K +V L+R+RNPWG EW GA+SD S EWN I E+ RL DDGEFWM+F+DF ++F KLEIC+L+P+S L++ A K KWE+ + G W + +AGGCRNF+ETF NPQ K+ + + D D + T I LMQK R RK G DL TIGY IYQ + +S+L K +F+ S A+S T++NLRE+ R KLP G Y+ +P+TF +EA F LRIFSE+ D K++D + I D P +E + + F+ ISG D E+ A+ELQ +L+ +R +N KF S +C +++++MD +G+LGF+EFK+ WD L+ W +F ++D D+SGT +SYE+R AL GF ++N V I+ RYA+ Q I FDD++
Sbjct: 39 KGVLFEDVDFPANDSSLFYSEKPSVPFVWKRPKEMVADPEFILGGASRTDVCQGDLGDCWLLAAIASLTLNDKILYRVVPPDQSFSTGYAGIFHFQFWQHNEWLDVVIDDRLPTFRNRLVFVHSADLNEFWSALLEKAYAKLNGSYEALKGGSTLEAMEDFTGGVTETYEI-RSAPANLFDILDKAIKKGSMLGCSIDITSSAETEAKTPQGLVKGHAYSITGADVVNFKGQKVKLIRIRNPWGQ-VEWNGAWSDNSSEWNIIGAAEKNRLSQASLDDGEFWMDFEDFKRHFDKLEICNLTPDS-LDDDA---KHKWEVTVHEGSWIRGSTAGGCRNFLETFWSNPQIKLSLTEQD--DGQKECTFIAALMQKNRRKLRKTGADLLTIGYTIYQCPGS---ESILSKDFFKFNPSKARSKTYVNLREISQRFKLPPGDYMFIPTTFEPHQEADFCLRIFSEKKADIKEMDGEVNI---DLPDPPAPTPPHKETEEERQFRTLFEQISGQDLEVSAEELQYVLNAVLRRTKNIKFKEMSLLSCNNIISLMDASGNGKLGFDEFKIFWDKLKTWIGIFMQYDLDKSGTMSSYELRLALKSAGFHLNNIVLQLIVLRYADDQFQIEFDDFL 652
BLAST of Calpain-B vs. Ensembl Xenopus
Match: tgfbrap1 (transforming growth factor beta receptor associated protein 1 [Source:Xenbase;Acc:XB-GENE-1009020]) HSP 1 Score: 571.622 bits (1472), Expect = 0.000e+0 Identity = 301/629 (47.85%), Postives = 403/629 (64.07%), Query Frame = 1
Query: 433 KNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIE-ADPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILDVSFK--RENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYI 2310
K LFED FPAN++SL+Y E P WKRPKEM + FI G SR DV QG+LGDCWLLAA++SL++ ++++V+P DQ F Y G F F+ WQ W DVV+DDRLPT R RLV++HS DLNEFWSALLEKAYAK+ GSYEALKGGS+ EAMEDFTGG+TE +++ SAP NL+DI+ K + + S++GCSI+ + EAK +GL+KGHAYSIT VN K +V L+R+RNPWG EW GA+SD S EWN I E+ RL DDGEFWM+F+DF ++F KLEIC+L+P+S L++ A K KWE+ + G W + +AGGCRNF+ETF NPQ K+ + + D D + T I LMQK R RK G DL TIGY IYQ + +S+L K +F+ S A+S T++NLRE+ R KLP G Y+ +P+TF +EA F LRIFSE+ D K++D + I D P +E + + F+ ISG Q+ +K + ++ F S+E R + +G+LGF+EFK+ WD L+ W +F ++D D+SGT +SYE+R AL GF ++N V I+ RYA+ Q I FDD++
Sbjct: 39 KGVLFEDVDFPANDSSLFYSEKPSVPFVWKRPKEMVADPEFILGGASRTDVCQGDLGDCWLLAAIASLTLNDKILYRVVPPDQSFSTGYAGIFHFQFWQHNEWLDVVIDDRLPTFRNRLVFVHSADLNEFWSALLEKAYAKLNGSYEALKGGSTLEAMEDFTGGVTETYEI-RSAPANLFDILDKAIKKGSMLGCSIDITSSAETEAKTPQGLVKGHAYSITGADVVNFKGQKVKLIRIRNPWGQ-VEWNGAWSDNSSEWNIIGAAEKNRLSQASLDDGEFWMDFEDFKRHFDKLEICNLTPDS-LDDDA---KHKWEVTVHEGSWIRGSTAGGCRNFLETFWSNPQIKLSLTEQD--DGQKECTFIAALMQKNRRKLRKTGADLLTIGYTIYQCPGS---ESILSKDFFKFNPSKARSKTYVNLREISQRFKLPPGDYMFIPTTFEPHQEADFCLRIFSEKKADIKEMDGEVNI---DLPDPPAPTPPHKETEEERQFRTLFEQISG-----------QVSTTQYKAVQGSYLFKNKSKE--RKGIG------NGKLGFDEFKIFWDKLKTWIGIFMQYDLDKSGTMSSYELRLALKSAGFHLNNIVLQLIVLRYADDQFQIEFDDFL 634
BLAST of Calpain-B vs. Ensembl Xenopus
Match: CAPN3 (calpain 3 [Source:NCBI gene;Acc:100497532]) HSP 1 Score: 570.466 bits (1469), Expect = 0.000e+0 Identity = 287/668 (42.96%), Postives = 428/668 (64.07%), Query Frame = 1
Query: 412 QKRMLKAKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEA-DPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDEN--DNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQM-KDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAP----------DWKKEIPPEVKREEDANNV--------------------KLQNAFKVISGADGEIDADELQQIL-DVSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYI 2310
+K+ L+ +N F+D FP + +SL+Y ++ P K EWKRP E+ +N FI G +R D+ QG+LGDCW LAA++ L++ ++ +VIP DQ+F +Y G F F+ W++G W DV++DD LPT LV+ S NEFWSALLEKAYAK+ GSYEALKGG+++EAMEDFTGG+TE ++L AP ++++IM K R SL+GCSI+ P ++E + GL+KGHAYS+T V+ K + LVR+RNPWG EW GA+ D + EW + E+ RL ++DGEFWM++ DF+K FTK EIC+L+P++ + + W + + G W + SAGGCRN+ +T+ NPQ+++K+++ D++ DNE + ++ LMQK R RK G +LFTIG+AIY++ K+ + L K +F AS AK ++IN+REVC R +LP +YVI+PST+ +E +F+LR+FSE+ S++++ K E P + KE+ + +ED + + +N F+ I+G D EI ADELQ +L +V K + K +GF+ E+CRSM+A+MDTD GRL +EF LW ++ W+ +F +FD DQSGT +S+E+R A+N GF ++N+++ I RYANK+ + FD +I
Sbjct: 56 RKKCLQ-RNFHFQDREFPPDESSLFYSQSFPIKFEWKRPPEICENPQFILGGANRTDICQGDLGDCWFLAAIACLTLNEKVLFRVIPPDQNFTDNYAGIFHFQFWRYGDWVDVIIDDYLPTYNNELVFTKSSQRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTEFYEL-KEAPKDMYNIMKKAFERGSLIGCSIDTLVPVQLETRMANGLVKGHAYSVTGVEETKLKGKPIKLVRLRNPWG-QVEWNGAWGDNAKEWTMVDKSEKTRLQHQVQEDGEFWMSYDDFMKNFTKAEICNLTPDALDSDSLQV----WTVSVNEGRWVRGCSAGGCRNYPDTYWTNPQYRLKLLEEDDDPADNEVVCSFVVALMQKNRRKDRKAGANLFTIGFAIYEVPKEMHGNNQHLQKDFFLYNASKAKCKSYINMREVCQRFRLPPSEYVIIPSTYEPHQEGEFILRVFSEKRNISEEVENKIEAEKPIPIIFVSDRSNSNKELNVDGATDEDQKKMDAEKKDKTSASTDTETEEEKQFRNIFQQIAGDDMEISADELQSVLNNVVNKHKTLKSSGFTLESCRSMIALMDTDGCGRLNLQEFYHLWQKIKQWQKIFLRFDSDQSGTISSFEMRNAINEAGFHLNNQLYDIITMRYANKRMDLDFDSFI 716
BLAST of Calpain-B vs. Ensembl Xenopus
Match: capn1 (calpain 1, (mu/I) large subunit [Source:NCBI gene;Acc:541455]) HSP 1 Score: 570.081 bits (1468), Expect = 0.000e+0 Identity = 298/672 (44.35%), Postives = 425/672 (63.24%), Query Frame = 1
Query: 328 KQTDSESIYGSRFHVSKSLKESKDWFYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPPQK-----IEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIE-ADPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDEN---DNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKS--LLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILD-VSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDY 2307
KQ D E G + K L + D ++ +LF+D FP + SL ++E P ++W RP ++ N FI DG +R D+ QG LGDCWLLAA++SL++ ++H+V+P Q F +Y G F F+LWQFG W DVV+DD LP G+LV++HS NEFWSALLEKAYAK G YEAL GGS+SE EDFTGG+TE +++ AP +L+ I+MK V R SLMGCSI+ T +EA K L+KGHAYS+T K +N + L+R+RNPWG EWTGA+SD S EWN + + + L + E DGEFWM F+DF++ F++LEIC+L+P++ K KW + NG W+K +AGGCRNF TF +NPQFK+K+ + D++ +E T ++ LMQK+ R R+ G+D+ TIG+A+Y++ + +S L + +F AS A+S FINLREV RHKLP G+Y++VPSTF E F++R FSE + S ++D+ ++PPE E + +N FK ++GAD EI A ELQ IL+ + K ++ + +GFS E+CRSMV +MD D +G+LG EF VLW+ ++ + TVF+KFD D+SG+ ++YE+R A+ GF+++N + IITRY+ + FD +
Sbjct: 20 KQRDQELGVGKHENAVKFLGQDYDRILHE---CLQNRTLFKDDTFPPSAYSLGFKELGPNSSKTYGVKWVRPSQLVSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNDTILHRVVPHGQSFQDNYAGVFHFQLWQFGEWVDVVIDDYLPVKDGKLVFVHSASGNEFWSALLEKAYAKANGCYEALSGGSTSEGFEDFTGGVTEWYEM-KKAPKDLFSIIMKAVERGSLMGCSIDITSATDMEAVTFKKLVKGHAYSVTGAKEINFRGRSQKLIRMRNPWGE-VEWTGAWSDNSSEWNSVDAADSQDLRIKME-DGEFWMAFEDFLREFSRLEICNLTPDA----LTARKYRKWNTTVFNGNWRKGSTAGGCRNFPATFWINPQFKIKLDEEDDDDDFGSEKGCTFLLALMQKDKRKERRFGKDMETIGFAVYEVPSQFHGQSGVHLKRDFFLQNASRARSEQFINLREVSTRHKLPPGEYIVVPSTFEPNVEGDFVVRFFSENKHGSVEMDDPLS-------ADLPPEEVLSEAEIDQGFKNLFKQLAGADNEISATELQSILNKIMSKHKDLRTSGFSLESCRSMVNLMDKDGNGKLGLVEFNVLWNKIKNYLTVFRKFDMDKSGSMSAYEMRLAVESAGFKLTNSLHQLIITRYSEPDMAVNFDSF 674
BLAST of Calpain-B vs. Ensembl Mouse
Match: Capn9 (calpain 9 [Source:MGI Symbol;Acc:MGI:1920897]) HSP 1 Score: 580.482 bits (1495), Expect = 0.000e+0 Identity = 296/648 (45.68%), Postives = 417/648 (64.35%), Query Frame = 1
Query: 373 SKSLKESKDWFYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEA-DPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILDVSF-KRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYI 2310
++++ S F ++ +LFED FPA+N SL+Y E P WKRP E+ + FI G +R D+ QGELGDCWLLAA++SL++ + +V+P DQ F Y G F F+ WQ W DVV+DDRLPT + RLV+LHS D NEFWSALLEKAYAK+ GSYEALKGGS+ EAMEDFTGG+ E F + AP + ++I+ K + R SL+GCSI+ + ++ EA+ GLIKGHAY++T + VN R+ L+RVRNPWG EW G +SD SPEW + E+KRLG T DDGEFWM F+DF +F K+EIC+L+P++ LE+ A +WE+ I G W + +AGGCRNF++TF NPQ K+ + + DE + T + LMQK+ R ++ G ++ TIGYAIYQ D L + +F+ AS A+S TFINLREV R +LP G Y+++PSTF +EA F LRIFSE+ ++ +DE +I P+ K + EE + + F+ ++G D E+ A+EL+ +L+ K+ KF S +CR+++++MDT +G+L FEEF+V WD L+ W +F +FD D+SGT +SYE+R AL GF++ + I+ RYA++ + FDDY+
Sbjct: 19 ARTIHSSGQSFEQLRQGCLQSGTLFEDADFPASNVSLFYSERPQVPFVWKRPGEIVEKPEFILGGATRTDICQGELGDCWLLAAIASLTLNQKALTRVVPQDQGFGSGYAGIFHFQFWQHSEWLDVVIDDRLPTFKDRLVFLHSADHNEFWSALLEKAYAKLNGSYEALKGGSAIEAMEDFTGGVAENFQI-REAPEDFFEILEKALKRGSLLGCSIDTLNASESEARTSLGLIKGHAYTVTGLDQVNFHGQRIKLIRVRNPWGQ-VEWNGPWSDSSPEWRSVDLEEQKRLGHTALDDGEFWMAFKDFKIHFDKVEICNLTPDA-LEDSA---LHRWEVTIHQGSWVRGSTAGGCRNFLDTFWTNPQIKLSLTERDE--GQEGCTFLAALMQKDRRRLKRFGANMLTIGYAIYQCPDK---DGHLSRDFFRYHASLARSKTFINLREVSERFQLPPGDYILIPSTFEPHQEADFCLRIFSEKRAVTRDLDENIDIDLPELPKP---TPQEEETEEEQQFRALFQRVAGEDMEVSAEELEYVLNAVLQKKTALKFKRLSLLSCRNIISLMDTSGNGKLEFEEFRVFWDKLKHWMDLFLQFDVDKSGTMSSYELRTALKAAGFQLGGHLLQLIVLRYADEDLQLDFDDYL 652
BLAST of Calpain-B vs. Ensembl Mouse
Match: Capn1 (calpain 1 [Source:MGI Symbol;Acc:MGI:88263]) HSP 1 Score: 562.762 bits (1449), Expect = 0.000e+0 Identity = 303/673 (45.02%), Postives = 438/673 (65.08%), Query Frame = 1
Query: 328 KQTDSESIYGSRFHVSKSLKESKDWFYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPPQK-----IEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIE-ADPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVK---VVDPDENDNENTG-TLIIGLMQKEMRSRRKEGQDLFTIGYAIYQM-KDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILD-VSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYI 2310
K+ D E G + K L +D+ + R L++ LF+D FP + SL ++E P I+WKRP E+ N FI DG +R D+ QG LGDCWLLAA++SL++ ++H+V+P Q F Y G F F+LWQFG W DVV+DD LPT G+LV++HS NEFWSALLEKAYAKV GSYEAL GG +SEA EDFTGG+TE +DL AP +L+ I++K + R SL+GCSI +D +EA K L++GHAYS+T K V + RVNL+R+RNPWG EW G +SD S EWN++ ER++L + E DGEFWM+F+DF++ FTKLEIC+L+P++ L+++ W G W++ +AGGCRN+ TF +NPQFK++ V D D+ DN +G + ++ LMQK R R+ G+D+ TIG+A+YQ+ ++ A L + +F AS A+S FINLREV R +LP G+Y++VPSTF +E FLLR FSE+ ++++D++ + PD K V EE+ ++ + F ++G D EI ELQ IL+ + K ++ + NGFS E+CRSMV +MD D +G+LG EF +LW+ +R + T+F+KFD D+SG+ ++YE+R A+ GF+++ ++ IITRY+ + FD+++
Sbjct: 20 KKRDKELGLGRHENAIKYL--GQDYETLRARCLQS-GVLFQDEAFPPVSHSLGFKELGPHSSKTYGIKWKRPTELMSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNETILHRVVPYGQSFQDGYAGIFHFQLWQFGEWVDVVIDDLLPTKDGKLVFVHSAQGNEFWSALLEKAYAKVNGSYEALSGGCTSEAFEDFTGGVTEWYDL-QKAPSDLYQIILKALERGSLLGCSINISDIRDLEAITFKNLVRGHAYSVTGAKQVTYQGQRVNLIRMRNPWG-EVEWKGPWSDSSYEWNKVDPYEREQLRVKME-DGEFWMSFRDFIREFTKLEICNLTPDA-LKSRT---LRNWNTTFYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLEEVDDADDYDNRESGCSFLLALMQKHRRRERRFGRDMETIGFAVYQVPRELAGQPVHLKRDFFLANASRAQSEHFINLREVSNRIRLPPGEYIVVPSTFEPNKEGDFLLRFFSEKKAGTQELDDQIQANLPDEK------VLSEEEIDD-NFKTLFSKLAGDDMEISVKELQTILNRIISKHKDLRTNGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLTIFRKFDLDKSGSMSAYEMRMAIEAAGFKLNKKLHELIITRYSEPDLAVDFDNFV 675
BLAST of Calpain-B vs. Ensembl Mouse
Match: Capn1 (calpain 1 [Source:MGI Symbol;Acc:MGI:88263]) HSP 1 Score: 562.762 bits (1449), Expect = 0.000e+0 Identity = 303/673 (45.02%), Postives = 438/673 (65.08%), Query Frame = 1
Query: 328 KQTDSESIYGSRFHVSKSLKESKDWFYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPPQK-----IEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIE-ADPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVK---VVDPDENDNENTG-TLIIGLMQKEMRSRRKEGQDLFTIGYAIYQM-KDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILD-VSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYI 2310
K+ D E G + K L +D+ + R L++ LF+D FP + SL ++E P I+WKRP E+ N FI DG +R D+ QG LGDCWLLAA++SL++ ++H+V+P Q F Y G F F+LWQFG W DVV+DD LPT G+LV++HS NEFWSALLEKAYAKV GSYEAL GG +SEA EDFTGG+TE +DL AP +L+ I++K + R SL+GCSI +D +EA K L++GHAYS+T K V + RVNL+R+RNPWG EW G +SD S EWN++ ER++L + E DGEFWM+F+DF++ FTKLEIC+L+P++ L+++ W G W++ +AGGCRN+ TF +NPQFK++ V D D+ DN +G + ++ LMQK R R+ G+D+ TIG+A+YQ+ ++ A L + +F AS A+S FINLREV R +LP G+Y++VPSTF +E FLLR FSE+ ++++D++ + PD K V EE+ ++ + F ++G D EI ELQ IL+ + K ++ + NGFS E+CRSMV +MD D +G+LG EF +LW+ +R + T+F+KFD D+SG+ ++YE+R A+ GF+++ ++ IITRY+ + FD+++
Sbjct: 20 KKRDKELGLGRHENAIKYL--GQDYETLRARCLQS-GVLFQDEAFPPVSHSLGFKELGPHSSKTYGIKWKRPTELMSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNETILHRVVPYGQSFQDGYAGIFHFQLWQFGEWVDVVIDDLLPTKDGKLVFVHSAQGNEFWSALLEKAYAKVNGSYEALSGGCTSEAFEDFTGGVTEWYDL-QKAPSDLYQIILKALERGSLLGCSINISDIRDLEAITFKNLVRGHAYSVTGAKQVTYQGQRVNLIRMRNPWG-EVEWKGPWSDSSYEWNKVDPYEREQLRVKME-DGEFWMSFRDFIREFTKLEICNLTPDA-LKSRT---LRNWNTTFYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLEEVDDADDYDNRESGCSFLLALMQKHRRRERRFGRDMETIGFAVYQVPRELAGQPVHLKRDFFLANASRAQSEHFINLREVSNRIRLPPGEYIVVPSTFEPNKEGDFLLRFFSEKKAGTQELDDQIQANLPDEK------VLSEEEIDD-NFKTLFSKLAGDDMEISVKELQTILNRIISKHKDLRTNGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLTIFRKFDLDKSGSMSAYEMRMAIEAAGFKLNKKLHELIITRYSEPDLAVDFDNFV 675
BLAST of Calpain-B vs. Ensembl Mouse
Match: Capn1 (calpain 1 [Source:MGI Symbol;Acc:MGI:88263]) HSP 1 Score: 562.762 bits (1449), Expect = 0.000e+0 Identity = 303/673 (45.02%), Postives = 438/673 (65.08%), Query Frame = 1
Query: 328 KQTDSESIYGSRFHVSKSLKESKDWFYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPPQK-----IEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIE-ADPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVK---VVDPDENDNENTG-TLIIGLMQKEMRSRRKEGQDLFTIGYAIYQM-KDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILD-VSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYI 2310
K+ D E G + K L +D+ + R L++ LF+D FP + SL ++E P I+WKRP E+ N FI DG +R D+ QG LGDCWLLAA++SL++ ++H+V+P Q F Y G F F+LWQFG W DVV+DD LPT G+LV++HS NEFWSALLEKAYAKV GSYEAL GG +SEA EDFTGG+TE +DL AP +L+ I++K + R SL+GCSI +D +EA K L++GHAYS+T K V + RVNL+R+RNPWG EW G +SD S EWN++ ER++L + E DGEFWM+F+DF++ FTKLEIC+L+P++ L+++ W G W++ +AGGCRN+ TF +NPQFK++ V D D+ DN +G + ++ LMQK R R+ G+D+ TIG+A+YQ+ ++ A L + +F AS A+S FINLREV R +LP G+Y++VPSTF +E FLLR FSE+ ++++D++ + PD K V EE+ ++ + F ++G D EI ELQ IL+ + K ++ + NGFS E+CRSMV +MD D +G+LG EF +LW+ +R + T+F+KFD D+SG+ ++YE+R A+ GF+++ ++ IITRY+ + FD+++
Sbjct: 20 KKRDKELGLGRHENAIKYL--GQDYETLRARCLQS-GVLFQDEAFPPVSHSLGFKELGPHSSKTYGIKWKRPTELMSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNETILHRVVPYGQSFQDGYAGIFHFQLWQFGEWVDVVIDDLLPTKDGKLVFVHSAQGNEFWSALLEKAYAKVNGSYEALSGGCTSEAFEDFTGGVTEWYDL-QKAPSDLYQIILKALERGSLLGCSINISDIRDLEAITFKNLVRGHAYSVTGAKQVTYQGQRVNLIRMRNPWG-EVEWKGPWSDSSYEWNKVDPYEREQLRVKME-DGEFWMSFRDFIREFTKLEICNLTPDA-LKSRT---LRNWNTTFYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLEEVDDADDYDNRESGCSFLLALMQKHRRRERRFGRDMETIGFAVYQVPRELAGQPVHLKRDFFLANASRAQSEHFINLREVSNRIRLPPGEYIVVPSTFEPNKEGDFLLRFFSEKKAGTQELDDQIQANLPDEK------VLSEEEIDD-NFKTLFSKLAGDDMEISVKELQTILNRIISKHKDLRTNGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLTIFRKFDLDKSGSMSAYEMRMAIEAAGFKLNKKLHELIITRYSEPDLAVDFDNFV 675
BLAST of Calpain-B vs. Ensembl Mouse
Match: Capn3 (calpain 3 [Source:MGI Symbol;Acc:MGI:107437]) HSP 1 Score: 561.607 bits (1446), Expect = 0.000e+0 Identity = 301/679 (44.33%), Postives = 434/679 (63.92%), Query Frame = 1
Query: 403 FYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEAD-PTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNEN--TGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQM-KDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIP-PEVKREEDANNVKLQNAFKVISGADGEIDADELQQILD-VSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYIXXXXXXXXXXXXXKAQQKTSKGEAIFPQDEFMQCVLY 2421
F +R K L+ DP FP + TSL+Y + P + WKRP E+ +N FI G +R D+ QG+LGDCW LAA++ L++ L+ +VIP DQ F +Y G F F+ W++G W DVV+DD LPT +LV+ S NEFWSALLEKAYAK+ GSYEALKGG+++EAMEDFTGG+TE F++ AP +++ IM K + R SLMGCSI+ P + E + GL+KGHAYS+T ++ K +V LVR+RNPWG EW G++SD +W+ + E+ RL +DGEFWM++ DFV +FTKLEIC+L+ ++ LE+ K W + + G W + SAGGCRNF +TF NPQ+++K+++ D++ ++ + ++ LMQK R RK G +LFTIG+AIY++ K+ +K L K +F AS A+S T+IN+REV R +LP +YVIVPST+ +E +F+LR+FSE+ S++ + + P +P P +E + +N F+ I+G D EI ADEL+ +L+ V K ++ K GF+ E+CRSM+A+MDTD SGRL +EF LW ++ W+ +FK +D D SGT NSYE+R A+N GF ++++++ I RYA+K I FD +I VRL +F F A K G E++Q +Y
Sbjct: 61 FEQLRRKCLEKKVLYLDPEFPPDETSLFYSQKFPIQFVWKRPPEICENPRFIIGGANRTDICQGDLGDCWFLAAIACLTLNERLLFRVIPHDQSFTENYAGIFHFQFWRYGDWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTEFFEI-KDAPSDMYKIMRKAIERGSLMGCSIDTIVPVQYETRMACGLVKGHAYSVTGLEEALFKGEKVKLVRLRNPWGQ-VEWNGSWSDGWKDWSFVDKDEKARLQHQVTEDGEFWMSYDDFVYHFTKLEICNLTADA-LESD---KLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDDPEDSEVICSFLVALMQKNRRKDRKLGANLFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASKARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEAENTISVDRP-----VPRPGHTDQESEEQQQFRNIFRQIAGDDMEICADELKNVLNTVVNKHKDLKTQGFTLESCRSMIALMDTDGSGRLNLQEFHHLWKKIKAWQKIFKHYDTDHSGTINSYEMRNAVNDAGFHLNSQLYDIITMRYADKHMNIDFDSFICCFVRLEGMFRAFNAFDKDGDGIIKLNVLEWLQLTMY 728
BLAST of Calpain-B vs. UniProt/SwissProt
Match: sp|Q9VT65|CANB_DROME (Calpain-B OS=Drosophila melanogaster OX=7227 GN=CalpB PE=1 SV=2) HSP 1 Score: 649.818 bits (1675), Expect = 0.000e+0 Identity = 322/687 (46.87%), Postives = 455/687 (66.23%), Query Frame = 1
Query: 391 SKDWFYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEADPTKVEAKAEKGLIKGHAYSITDVK----SVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQID------EKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILDVSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYIXXXXXXXXXXXXXKAQQKTSKGEAIFPQDEFMQCVLY 2421
SK F + + A ++FEDP FPA N SL Y P + EW RP ++ + F +G SRFDV QGELGDCWLLAA ++L+ L +VIP DQDF +Y G F F+ WQ+G W +VV+DDRLPT G L+Y+HS + NEFWSALLEKAYAK+ GSYEALKGG++ EAMEDFTGG+TE +D+ APPNL+ IMMK R S+MGCS+E DP +EA+ +GLI+GHAYSIT V S +++G++ ++R+RNPWGN EW+G +SD SPEW I + ++ +GL F+ DGEFWM+FQDF+ +F ++EIC+LSP+S E++ + KWEM + GEW V+AGGCRNF+ETF NPQ+ + + DPD+ D++ T I+ LMQK RS+R G D TIG+AIY + D +F+ AS A+SP FIN REVC R KLP G Y+IVPSTF+ EE +F++R+FSE + ++ D E + +AP PP K E+D + L+ F ++G+D E+D EL++ILD S + +GFS++ RSMVAM+D D+SGRLGFEEF+ L D+ W+ VFK +D ++G+ + + +R ALN G+ ++NR+ A+ RY +++G I FDD+++ +++RT F+ + + A F D++++ +Y
Sbjct: 242 SKGDFQSLRDSCLANGTMFEDPDFPATNASLMYSRRPDRYYEWLRPGDIADDPQFFVEGYSRFDVQQGELGDCWLLAAAANLTQDSTLFFRVIPPDQDFQENYAGIFHFKFWQYGKWVEVVIDDRLPTYNGELIYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGTTCEAMEDFTGGVTEWYDI-KEAPPNLFSIMMKAAERGSMMGCSLEPDPHVLEAETPQGLIRGHAYSITKVCLMDISTPNRQGKLPMIRMRNPWGNDAEWSGPWSDSSPEWRFIPEHTKEEIGLNFDRDGEFWMSFQDFLNHFDRVEICNLSPDSLTEDQQHSSRRKWEMSMFEGEWTSGVTAGGCRNFLETFWHNPQYIISLEDPDDEDDDGKCTAIVALMQKNRRSKRNVGIDCLTIGFAIYHLTDRDMQVKPQGLNFFKYRASVARSPHFINTREVCARFKLPPGHYLIVPSTFDPNEEGEFIIRVFSETRNNMEENDDEVGFGETDDRIAPSLP---PPTPKEEDDPQRIALRRLFDSVAGSDEEVDWQELKRILDHSMRDVMVGSDGFSKDAVRSMVAMLDKDRSGRLGFEEFEALLTDIAKWRAVFKLYDTRRTGSIDGFHLRGALNSAGYHLNNRLLNALAHRYGSREGQIPFDDFLMCAIKVRTFIEMFRERDTDNSDTAFFNLDDWLERTIY 924
BLAST of Calpain-B vs. UniProt/SwissProt
Match: sp|O35920|CAN9_RAT (Calpain-9 OS=Rattus norvegicus OX=10116 GN=Capn9 PE=2 SV=2) HSP 1 Score: 598.201 bits (1541), Expect = 0.000e+0 Identity = 306/666 (45.95%), Postives = 428/666 (64.26%), Query Frame = 1
Query: 319 PDPKQTDSESIYGSRFHVSKSLKESKDWFYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEA-DPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILDVSF-KRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYI 2310
P P D+ +++ S H + L++S +LFED FPA+N+SL+Y E P WKRP E+ +N FI G +R D+ QGELGDCWLLAA++SL++ + +V+P DQ F Y G F F+ WQ W DVV+DDRLPT R RLV+LHS D NEFWSALLEKAYAK+ GSYEALKGGS+ EAMEDFTGG+ E F + AP + ++I+ K + R SL+GCSI+ + ++ EA+ GLIKGHAY++T + VN R+ L+RVRNPWG EW G +SD SPEW +S E+KRLG T DDGEFWM F+DF +F K+EIC+L+P++ +N KWE+ I G W + +AGGCRNF++TF NPQ K+ + + DE + T + LMQK+ R ++ G ++ TIGYAIYQ D L++ +F+ AS A+S TFINLREV GR +LP G Y+++PSTF +EA F LRIFSE+ ++ +DE +I P+ K P + EE + + F+ I+G D E+ A+EL+ +L+ K+ KF S +CR+++++MDT +G++ FEEF+V WD LR W +F +FD D+SGT +SYE+R AL GF++ + + I+ RYA++ + FDDY+
Sbjct: 12 PQPVPRDARTVHSSG-HSFEQLRQS----------CLQSGTLFEDADFPASNSSLFYSERPQVPFVWKRPGEIVENPEFILGGATRTDICQGELGDCWLLAAIASLTLNQKALARVVPQDQGFGSGYAGIFHFQFWQHSEWLDVVIDDRLPTFRDRLVFLHSADHNEFWSALLEKAYAKLNGSYEALKGGSAIEAMEDFTGGVAENFQI-REAPEDFYEILEKALRRGSLLGCSIDTLNASESEARTPLGLIKGHAYTVTGLDQVNFHGQRIKLIRVRNPWGQ-VEWNGPWSDSSPEWRSMSLEEQKRLGHTALDDGEFWMAFEDFKTHFDKVEICNLTPDALEDNTL----HKWEVTIHQGSWVRGSTAGGCRNFLDTFWTNPQIKLSLTERDE--GQEGCTFLAALMQKDRRRLKRFGANMLTIGYAIYQCPDK---DGHLNRDFFRYHASLARSKTFINLREVSGRFQLPPGDYILIPSTFEPHQEADFCLRIFSEKKAVTQDLDENIDIDLPELPKPTP---QEEETEEERQFRALFRRIAGEDMEVSAEELEYVLNAVLQKKTALKFKRLSLLSCRNIISLMDTSGNGKMEFEEFRVFWDKLRYWMDLFLQFDVDKSGTMSSYELRTALKAAGFQLGSHLLQLIVLRYADENLQLDFDDYL 652
BLAST of Calpain-B vs. UniProt/SwissProt
Match: sp|Q9D805|CAN9_MOUSE (Calpain-9 OS=Mus musculus OX=10090 GN=Capn9 PE=2 SV=2) HSP 1 Score: 580.482 bits (1495), Expect = 0.000e+0 Identity = 296/648 (45.68%), Postives = 417/648 (64.35%), Query Frame = 1
Query: 373 SKSLKESKDWFYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEA-DPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILDVSF-KRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYI 2310
++++ S F ++ +LFED FPA+N SL+Y E P WKRP E+ + FI G +R D+ QGELGDCWLLAA++SL++ + +V+P DQ F Y G F F+ WQ W DVV+DDRLPT + RLV+LHS D NEFWSALLEKAYAK+ GSYEALKGGS+ EAMEDFTGG+ E F + AP + ++I+ K + R SL+GCSI+ + ++ EA+ GLIKGHAY++T + VN R+ L+RVRNPWG EW G +SD SPEW + E+KRLG T DDGEFWM F+DF +F K+EIC+L+P++ LE+ A +WE+ I G W + +AGGCRNF++TF NPQ K+ + + DE + T + LMQK+ R ++ G ++ TIGYAIYQ D L + +F+ AS A+S TFINLREV R +LP G Y+++PSTF +EA F LRIFSE+ ++ +DE +I P+ K + EE + + F+ ++G D E+ A+EL+ +L+ K+ KF S +CR+++++MDT +G+L FEEF+V WD L+ W +F +FD D+SGT +SYE+R AL GF++ + I+ RYA++ + FDDY+
Sbjct: 19 ARTIHSSGQSFEQLRQGCLQSGTLFEDADFPASNVSLFYSERPQVPFVWKRPGEIVEKPEFILGGATRTDICQGELGDCWLLAAIASLTLNQKALTRVVPQDQGFGSGYAGIFHFQFWQHSEWLDVVIDDRLPTFKDRLVFLHSADHNEFWSALLEKAYAKLNGSYEALKGGSAIEAMEDFTGGVAENFQI-REAPEDFFEILEKALKRGSLLGCSIDTLNASESEARTSLGLIKGHAYTVTGLDQVNFHGQRIKLIRVRNPWGQ-VEWNGPWSDSSPEWRSVDLEEQKRLGHTALDDGEFWMAFKDFKIHFDKVEICNLTPDA-LEDSA---LHRWEVTIHQGSWVRGSTAGGCRNFLDTFWTNPQIKLSLTERDE--GQEGCTFLAALMQKDRRRLKRFGANMLTIGYAIYQCPDK---DGHLSRDFFRYHASLARSKTFINLREVSERFQLPPGDYILIPSTFEPHQEADFCLRIFSEKRAVTRDLDENIDIDLPELPKP---TPQEEETEEEQQFRALFQRVAGEDMEVSAEELEYVLNAVLQKKTALKFKRLSLLSCRNIISLMDTSGNGKLEFEEFRVFWDKLKHWMDLFLQFDVDKSGTMSSYELRTALKAAGFQLGGHLLQLIVLRYADEDLQLDFDDYL 652
BLAST of Calpain-B vs. UniProt/SwissProt
Match: sp|O14815|CAN9_HUMAN (Calpain-9 OS=Homo sapiens OX=9606 GN=CAPN9 PE=1 SV=1) HSP 1 Score: 573.548 bits (1477), Expect = 0.000e+0 Identity = 292/638 (45.77%), Postives = 417/638 (65.36%), Query Frame = 1
Query: 403 FYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEA-DPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILDVSFK-RENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYI 2310
F ++ + +LFED FPA+N+SL+Y E P WKRP E+ +N FI G +R D+ QGELGDCWLLAA++SL++ + +VIP DQ F Y G F F+ WQ W DVV+DDRLPT R RLV+LHS D NEFWSALLEKAYAK+ GSYEALKGGS+ EAMEDFTGG+ E F AP N ++I+ K + R SL+GC I+ + EA+ GLIKGHAYS+T + V+ + R+ L+R+RNPWG EW G++SD SPEW + E+KRL T DDGEFWM F+DF +F K+EIC+L+P++ LE A KWE+ + G W + +AGGCRNF++TF NPQ K+ + + DE E + ++ LMQK+ R ++ G ++ TIGYAIY+ D L+K +F+ AS A+S TFINLREV R KLP G+Y+++PSTF +EA F LRIFSE+ ++ +D +I D + P +E + + F+ ++G D E+ A+EL+ +L+ + +++ KF S +C++++++MDT +G+L F+EFKV WD L+ W +F +FD D+SGT ++YE+R AL GF++S+ + I+ RYA+++ + FDD++
Sbjct: 29 FEQMRQECLQRGTLFEDADFPASNSSLFYSERPQIPFVWKRPGEIVKNPEFILGGATRTDICQGELGDCWLLAAIASLTLNQKALARVIPQDQSFGPGYAGIFHFQFWQHSEWLDVVIDDRLPTFRDRLVFLHSADHNEFWSALLEKAYAKLNGSYEALKGGSAIEAMEDFTGGVAETFQT-KEAPENFYEILEKALKRGSLLGCFIDTRSAAESEARTPFGLIKGHAYSVTGIDQVSFRGQRIELIRIRNPWGQ-VEWNGSWSDSSPEWRSVGPAEQKRLCHTALDDGEFWMAFKDFKAHFDKVEICNLTPDA-LEEDA---IHKWEVTVHQGSWVRGSTAGGCRNFLDTFWTNPQIKLSLTEKDEGQEE--CSFLVALMQKDRRKLKRFGANVLTIGYAIYECPDK---DEHLNKDFFRYHASRARSKTFINLREVSDRFKLPPGEYILIPSTFEPHQEADFCLRIFSEKKAITRDMDGNVDI---DLPEPPKPTPPDQETEEEQRFRALFEQVAGEDMEVTAEELEYVLNAVLQKKKDIKFKKLSLISCKNIISLMDTSGNGKLEFDEFKVFWDKLKQWINLFLRFDADKSGTMSTYELRTALKAAGFQLSSHLLQLIVLRYADEELQLDFDDFL 652
BLAST of Calpain-B vs. UniProt/SwissProt
Match: sp|P97571|CAN1_RAT (Calpain-1 catalytic subunit OS=Rattus norvegicus OX=10116 GN=Capn1 PE=1 SV=1) HSP 1 Score: 564.688 bits (1454), Expect = 0.000e+0 Identity = 303/673 (45.02%), Postives = 435/673 (64.64%), Query Frame = 1
Query: 328 KQTDSESIYGSRFHVSKSLKESKDWFYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPPQK-----IEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIE-ADPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVK---VVDPDENDNENTG-TLIIGLMQKEMRSRRKEGQDLFTIGYAIYQM-KDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILD-VSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYI 2310
KQ D E G + K L + + N + LF+D FP + SL ++E P I+WKRP E+ N FI DG +R D+ QG LGDCWLLAA++SL++ ++H+V+P Q F Y G F F+LWQFG W DVVVDD LPT G+LV++HS NEFWSALLEKAYAKV GSYEAL GG +SEA EDFTGG+TE +DL AP +L+ I++K + R SL+GCSI +D +EA K L++GHAYS+TD K V + RVNL+R+RNPWG EW G +SD S EWN++ ER++L + E DGEFWM+F+DF++ FTKLEIC+L+P++ L+++ W G W++ +AGGCRN+ TF +NPQFK++ V D D+ D+ +G + ++ LMQK R R+ G+D+ TIG+A+YQ+ ++ A L + +F AS A+S FINLREV R +LP G+Y++VPSTF +E FLLR FSE+ ++++D++ + PD K V EE+ ++ + F ++G D EI ELQ IL+ + K ++ + NGFS E+CRSMV +MD D +G+LG EF +LW+ +R + T+F+KFD D+SG+ ++YE+R A+ GF+++ ++ IITRY+ + FD+++
Sbjct: 20 KQRDKELGLGRHENAIKYLGQD---YENLRARCLQNGVLFQDDAFPPVSHSLGFKELGPNSSKTYGIKWKRPTELLSNPQFIVDGATRTDICQGALGDCWLLAAIASLTLNETILHRVVPYGQSFQEGYAGIFHFQLWQFGEWVDVVVDDLLPTKDGKLVFVHSAQGNEFWSALLEKAYAKVNGSYEALSGGCTSEAFEDFTGGVTEWYDL-QKAPSDLYQIILKALERGSLLGCSINISDIRDLEAITFKNLVRGHAYSVTDAKQVTYQGQRVNLIRMRNPWG-EVEWKGPWSDNSYEWNKVDPYEREQLRVKME-DGEFWMSFRDFIREFTKLEICNLTPDA-LKSRT---LRNWNTTFYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLEEVDDADDYDSRESGCSFLLALMQKHRRRERRFGRDMETIGFAVYQVPRELAGQPVHLKRDFFLANASRAQSEHFINLREVSNRIRLPPGEYIVVPSTFEPNKEGDFLLRFFSEKKAGTQELDDQIQANLPDEK------VLSEEEIDD-NFKTLFSKLAGDDMEISVKELQTILNRIISKHKDLRTNGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLTIFRKFDLDKSGSMSAYEMRMAIEAAGFKLNKKLHELIITRYSEPDLAVDFDNFV 675
BLAST of Calpain-B vs. TrEMBL
Match: A0A1I8HRD3 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=3 SV=1) HSP 1 Score: 824.313 bits (2128), Expect = 0.000e+0 Identity = 415/720 (57.64%), Postives = 525/720 (72.92%), Query Frame = 1
Query: 295 INRFTPLSPDPKQTDSESIYGSRFHVSKSLKESKDWFYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDF-DRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEADPTKVEAKAEKGLIKGHAYSITDVKSV----NSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGI---KKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPP--EVKREEDANNVKLQNAFKVISGADGEIDADELQQILDVSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYIXXXXXXXXXXXXXKAQQKTSKGEAIFPQDEFMQCVLYM 2424
NR P S + + S S+ S + K F K+ L +K L+EDP FPA S++Y PP+ EWKRP E+ N F G SRFDV QGELGDCWLLAAV+SLSM+P L V+P DQDF D DY G FR WQFG W DV+VDDRLPT RLV++HS D NEFWSALLEKAYAK+ GSYEALKGGS+SEAMEDFTGGITE +DL S PP+L+ M+K R+SLMGCSIEADP ++EA+ GLI GHAYSITDV++V K+G V L+R+RNPWGN EW GA+ D SPEW+ +S+ E+K++GL+F+DDGEFWM+FQDF F KLEICHL P+S E + G K +WE N W+K VSAGGCRN+++TF NPQF+VKV+DPDE+D+EN G++I+GLMQK MR +R EG DL TIGYAIY++KD D LD ++F+ TAS AK+P FINLREVCGRHKL G YVIVPSTF +EA+FLLRI+SE +S ++D+ T + +E+ P E +E A L+ AF+ I+G D E+DA EL+ I++ +F++E FKF+GFS ETCRSMVA+MD DQSG+LGF+EFK LW+DLRLWKTVFKK+D D+SG FNSYE+RQALN +GFRV+N++F+ ++ RYA ++GII FDDY+L VRL+TVF TFKAQ K +G+A F Q+EF+Q ++Y+
Sbjct: 4 FNRNPPASAAASRGPATSRAASKKSAEISSGDEK--FRALKKELSSKKQLYEDPDFPAQPKSIFYSRQPPRPFEWKRPHELCDNPRFFVGGASRFDVRQGELGDCWLLAAVASLSMHPQLFDLVVPQDQDFTDADYCGVVRFRFWQFGEWTDVLVDDRLPTYNNRLVFMHSTDSNEFWSALLEKAYAKMMGSYEALKGGSASEAMEDFTGGITEFYDLRQSTPPSLFQTMVKANKRSSLMGCSIEADPNQIEAQLSNGLIMGHAYSITDVRTVEVNLRGKKGAVQLLRIRNPWGNEAEWKGAWGDSSPEWSILSEDEKKKMGLSFDDDGEFWMSFQDFCSNFQKLEICHLGPDSLNEEQLGAPDKNKRRWEALTHNSAWRKRVSAGGCRNYLDTFWTNPQFRVKVIDPDEDDDENKGSIIVGLMQKNMRKKRSEGLDLHTIGYAIYRIKDG--DDRPLDLKFFKCTASVAKAPAFINLREVCGRHKLDPGTYVIVPSTFEPNQEAEFLLRIYSETKNESGEMDDVTTV------EEVQPDREPTAQEKAQEKALKEAFQKIAGEDMEVDAYELRDIVNTAFQKE-FKFDGFSVETCRSMVALMDVDQSGKLGFDEFKTLWNDLRLWKTVFKKYDSDKSGNFNSYELRQALNAVGFRVNNKIFSCLVMRYATREGIIEFDDYVLCCVRLKTVFETFKAQPKDGQGKATFSQEEFLQTIIYV 712
BLAST of Calpain-B vs. TrEMBL
Match: A0A267EIH1 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig019924g1 PE=3 SV=1) HSP 1 Score: 822.387 bits (2123), Expect = 0.000e+0 Identity = 414/720 (57.50%), Postives = 525/720 (72.92%), Query Frame = 1
Query: 295 INRFTPLSPDPKQTDSESIYGSRFHVSKSLKESKDWFYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDF-DRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEADPTKVEAKAEKGLIKGHAYSITDVKSV----NSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGI---KKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPP--EVKREEDANNVKLQNAFKVISGADGEIDADELQQILDVSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYIXXXXXXXXXXXXXKAQQKTSKGEAIFPQDEFMQCVLYM 2424
NR P S + + S S+ S + K F K+ L +K L+EDP FPA S++Y PP+ EWKRP E+ N F G SRFDV QGELGDCWLLAAV+SLSM+P L V+P DQDF D DY G FR WQFG W DV+VDDRLPT RLV++HS D NEFWSALLEKAYAK+ GSYEALKGGS+SEAMEDFTGGITE +DL + PP+L+ M+K R+SLMGCSIEADP ++EA+ GLI GHAYSITDV++V K+G V L+R+RNPWGN EW GA+ D SPEW+ +S+ E+K++GL+F+DDGEFWM+FQDF F KLEICHL P+S E + G K +WE N W+K VSAGGCRN+++TF NPQF+VKV+DPDE+D+EN G++I+GLMQK MR +R EG DL TIGYAIY++KD D LD ++F+ TAS AK+P FINLREVCGRHKL G YVIVPSTF +EA+FLLRI+SE +S ++D+ T + +E+ P E +E A L+ AF+ I+G D E+DA EL+ I++ +F++E FKF+GFS ETCRSMVA+MD DQSG+LGF+EFK LW+DLRLWKTVFKK+D D+SG FNSYE+RQALN +GFRV+N++F+ ++ RYA ++GII FDDY+L VRL+TVF TFKAQ K +G+A F Q+EF+Q ++Y+
Sbjct: 4 FNRNPPASAAASRGPATSRAASKKSAEISSGDEK--FRALKKELSSKKQLYEDPDFPAQPKSIFYSRQPPRPFEWKRPHELCDNPRFFVGGASRFDVRQGELGDCWLLAAVASLSMHPQLFDLVVPQDQDFTDADYCGVVRFRFWQFGEWTDVLVDDRLPTYNNRLVFMHSTDSNEFWSALLEKAYAKMMGSYEALKGGSASEAMEDFTGGITEFYDLRQNTPPSLFQTMVKANKRSSLMGCSIEADPNQIEAQLSNGLIMGHAYSITDVQTVEVNLRGKKGAVQLLRIRNPWGNEAEWKGAWGDSSPEWSILSEDEKKKMGLSFDDDGEFWMSFQDFCSNFQKLEICHLGPDSLNEEQLGAPDKNKRRWEALTHNSAWRKRVSAGGCRNYLDTFWTNPQFRVKVIDPDEDDDENKGSIIVGLMQKNMRKKRSEGLDLHTIGYAIYRIKDG--DDRPLDLKFFKCTASVAKAPAFINLREVCGRHKLDPGTYVIVPSTFEPNQEAEFLLRIYSETKNESGEMDDVTTV------EEVQPDREPTAQEKAQEKALKEAFQKIAGEDMEVDAYELRDIVNTAFQKE-FKFDGFSVETCRSMVALMDVDQSGKLGFDEFKTLWNDLRLWKTVFKKYDSDKSGNFNSYELRQALNAVGFRVNNKIFSCLVMRYATREGIIEFDDYVLCCVRLKTVFETFKAQPKDGQGKATFSQEEFLQTIIYV 712
BLAST of Calpain-B vs. TrEMBL
Match: A0A5J4NR82 (Calpain, invertebrate OS=Paragonimus westermani OX=34504 GN=DEA37_0012115 PE=3 SV=1) HSP 1 Score: 778.474 bits (2009), Expect = 0.000e+0 Identity = 389/747 (52.07%), Postives = 509/747 (68.14%), Query Frame = 1
Query: 208 IVFSPIGFREMPRFNDSSNTNESVGSRYSINRFTPLSPDPKQTDSESIYGSRFHVSKSLKESKDWFYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEADPTKVEAKAEKGLIKGHAYSITDVKSVN----SKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILDVSFKRENFKFNGFSRETCRSMVAMMDT----DQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYIXXXXXXXXXXXXXKAQQKTSKGEAIFPQDEFMQCVLYM 2424
I F P G + PRF D V R + N + PD T ++ + +R S S D K L+ + LFEDP FPA + SLYY +PP+K++W RP ++ N FI G SRFDV QGELGDCWLLAA++ LSM+ L Q++P DQ F +Y G F F W +G W +VVVDDRLPTVRG L+Y+HS + NEFWSAL+EKAYAK+ GSYEALKGG++SEA+EDFTGGI EMF+L PP+L IM+K R SLM CSI+ADP ++EA+ GLI GHAYS+T VK VN + G + LVRVRNPWGN EW GA+SDKS EW+ +SD R+ +G+TF+DDGEFWM+++DFV+ F KLEICHL P+S L ++ + WEMC+E G W+ VSAGGCRNF+ETF +NPQ++V VVDPDEND++N GT+I+GLMQK R RK+G+DL TIGYA+Y++ + LD +F+ AS A+SP FINLREVCGRHKL G YVI+PSTF EEA F+LR+FSE+P S+++DE + P E + E L+ AF ++G DGEID +EL+ IL+V+F R+ FKF+GF+ E+CRSMVAMMD D+S L FEEFK LW+ LRLWKT FKKFD DQSG NS+E+R AL +GF V+N +F ++ R++ + G I FDDY++ RL+T+F + + K ++ +A+F + F+ VLYM
Sbjct: 38 IEFRPTGIIQFPRFKDPR-----VALRNAQNNKVVIPPDYGITSNKPVPSTRGGDLASTSLSYDQL---KAQLQQQGRLFEDPEFPAVDGSLYYSRSPPRKVDWLRPHQICSNPEFITGGASRFDVRQGELGDCWLLAAIACLSMHKNLFEQIVPEDQSFTSEYCGLFRFHFWHYGEWKEVVVDDRLPTVRGSLIYIHSTEKNEFWSALMEKAYAKLCGSYEALKGGTTSEALEDFTGGIAEMFELRDKCPPDLLKIMLKSQERCSLMACSIQADPNRIEAELPNGLIMGHAYSVTSVKVVNVSIPGRSGDIPLVRVRNPWGNEAEWKGAWSDKSREWSLLSDATRREVGITFDDDGEFWMSYEDFVRNFEKLEICHLGPQS-LGGESETRGRHWEMCVEQGSWKPRVSAGGCRNFLETFWINPQYRVTVVDPDENDDDNLGTMIVGLMQKNRRCLRKQGEDLLTIGYAVYELPQTS--GGTLDMNFFKYNASKARSPAFINLREVCGRHKLKPGNYVIIPSTFQPNEEADFMLRVFSEKPQTSEELDENVGVKDPIATVSTLNEQQVE------ALKRAFTKVAGEDGEIDCEELRDILNVAFTRD-FKFDGFTLESCRSMVAMMDVSFAFDRSSMLSFEEFKNLWNLLRLWKTAFKKFDVDQSGCMNSFELRNALKAVGFSVNNAIFATLVMRFSRRDGSILFDDYVICCARLKTLFDIYASTSKNTEDKAVFDETSFVNTVLYM 766
BLAST of Calpain-B vs. TrEMBL
Match: A0A4S2LND8 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_007626 PE=3 SV=1) HSP 1 Score: 778.089 bits (2008), Expect = 0.000e+0 Identity = 388/731 (53.08%), Postives = 505/731 (69.08%), Query Frame = 1
Query: 235 EMPRFNDSSNTNESVGSRYSINRFTPLSPDPKQTDSESIYGSRFHVSKSLKESKDWFYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEADPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGR-VNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILDVSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYIXXXXXXXXXXXXXKAQQKTSKGEAIFPQDEFMQCVLYM 2424
+ PRF D + R + + L D +T + + +R S +S D K L + LFEDP FP ++SLYY + PP+ ++W RP E+ QN FI G SRFDV QG+LGDCWLLAA++ LSM+ L QV+P DQ F Y G F F W+FG W +VVVDDRLPT L+Y+HS + NEFWSAL+EKAYAK+ GSYEALKGG++SEA+EDFTGGI EMF+L S PP+L+ IM+K R SLM CSI+ADP + EA+ GLI GHAYS+T VK +++ R V LVRVRNPWG+ EW GA+SDKSPEWN+I R+ +GLTFEDDGEFWM++ DF+ F KLEICHL P+S L + KWEMC+E+G W VSAGGCRNF+ETF +NPQF V +VDPDE+D+ N GT+IIGLMQK R RK+G+DL TIGYAIY + ++ LD +F+ AS A+SP FINLREVCGRHKLP G+Y I+PSTF EEA+F+LRIF+E P S++ID+ + A D I P + + +A L+ AF ++G DGEID++EL+ IL+V+F R +FKF+GF+ E+CRSM++MMD D+SG L F EFK LW+ LRLWKT FKKFD D+SG NS+E+R AL +GF ++N +F ++ R+A + G I FDDY++ RL+T+F FKA KT++G AIF + F+ +LYM
Sbjct: 46 KFPRFEDPR-----IALRKAKDNKVVLPFDAGETAKKPVPVTRGGALASTTQSYD---ELKVQLLQQGRLFEDPEFPPLDSSLYYSQPPPRPVQWLRPHEICQNPEFITGGASRFDVRQGQLGDCWLLAAIACLSMHKELFEQVVPGDQTFGSGYCGLFRFHFWRFGEWKEVVVDDRLPTYGKSLIYIHSTEKNEFWSALMEKAYAKLCGSYEALKGGTTSEALEDFTGGIAEMFELRSKCPPDLFKIMLKSQERCSLMACSIQADPNRYEARLANGLIMGHAYSVTSVKMIDTHTARQVTLVRVRNPWGDEAEWKGAWSDKSPEWNQIPAETRQLVGLTFEDDGEFWMSYTDFISNFEKLEICHLGPQS-LGGAGELPGRKWEMCVEHGSWVPRVSAGGCRNFLETFWINPQFSVNLVDPDEDDDVNEGTMIIGLMQKHRRKLRKQGEDLLTIGYAIYALPES--HSGTLDMNFFKYNASKARSPAFINLREVCGRHKLPPGRYAIIPSTFQPNEEAEFMLRIFAEHPQTSEEIDDA--VAAADTSALIQPLNEAQVEA----LKRAFTKVAGVDGEIDSEELRDILNVAFTR-DFKFDGFTLESCRSMISMMDFDRSGMLSFNEFKTLWNLLRLWKTAFKKFDVDKSGCMNSFELRNALKAVGFSINNSIFNTLVMRFARRDGSIAFDDYVICCARLQTLFEIFKASPKTTEGRAIFDETNFVNTLLYM 758
BLAST of Calpain-B vs. TrEMBL
Match: A0A4S2LFH5 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_007626 PE=3 SV=1) HSP 1 Score: 777.704 bits (2007), Expect = 0.000e+0 Identity = 388/731 (53.08%), Postives = 505/731 (69.08%), Query Frame = 1
Query: 235 EMPRFNDSSNTNESVGSRYSINRFTPLSPDPKQTDSESIYGSRFHVSKSLKESKDWFYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEADPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGR-VNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILDVSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYIXXXXXXXXXXXXXKAQQKTSKGEAIFPQDEFMQCVLYM 2424
+ PRF D + R + + L D +T + + +R S +S D K L + LFEDP FP ++SLYY + PP+ ++W RP E+ QN FI G SRFDV QG+LGDCWLLAA++ LSM+ L QV+P DQ F Y G F F W+FG W +VVVDDRLPT L+Y+HS + NEFWSAL+EKAYAK+ GSYEALKGG++SEA+EDFTGGI EMF+L S PP+L+ IM+K R SLM CSI+ADP + EA+ GLI GHAYS+T VK +++ R V LVRVRNPWG+ EW GA+SDKSPEWN+I R+ +GLTFEDDGEFWM++ DF+ F KLEICHL P+S L + KWEMC+E+G W VSAGGCRNF+ETF +NPQF V +VDPDE+D+ N GT+IIGLMQK R RK+G+DL TIGYAIY + ++ LD +F+ AS A+SP FINLREVCGRHKLP G+Y I+PSTF EEA+F+LRIF+E P S++ID+ + A D I P + + +A L+ AF ++G DGEID++EL+ IL+V+F R +FKF+GF+ E+CRSM++MMD D+SG L F EFK LW+ LRLWKT FKKFD D+SG NS+E+R AL +GF ++N +F ++ R+A + G I FDDY++ RL+T+F FKA KT++G AIF + F+ +LYM
Sbjct: 36 KFPRFEDPR-----IALRKAKDNKVVLPFDAGETAKKPVPVTRGGALASTTQSYD---ELKVQLLQQGRLFEDPEFPPLDSSLYYSQPPPRPVQWLRPHEICQNPEFITGGASRFDVRQGQLGDCWLLAAIACLSMHKELFEQVVPGDQTFGSGYCGLFRFHFWRFGEWKEVVVDDRLPTYGKSLIYIHSTEKNEFWSALMEKAYAKLCGSYEALKGGTTSEALEDFTGGIAEMFELRSKCPPDLFKIMLKSQERCSLMACSIQADPNRYEARLANGLIMGHAYSVTSVKMIDTHTARQVTLVRVRNPWGDEAEWKGAWSDKSPEWNQIPAETRQLVGLTFEDDGEFWMSYTDFISNFEKLEICHLGPQS-LGGAGELPGRKWEMCVEHGSWVPRVSAGGCRNFLETFWINPQFSVNLVDPDEDDDVNEGTMIIGLMQKHRRKLRKQGEDLLTIGYAIYALPES--HSGTLDMNFFKYNASKARSPAFINLREVCGRHKLPPGRYAIIPSTFQPNEEAEFMLRIFAEHPQTSEEIDDA--VAAADTSALIQPLNEAQVEA----LKRAFTKVAGVDGEIDSEELRDILNVAFTR-DFKFDGFTLESCRSMISMMDFDRSGMLSFNEFKTLWNLLRLWKTAFKKFDVDKSGCMNSFELRNALKAVGFSINNSIFNTLVMRFARRDGSIAFDDYVICCARLQTLFEIFKASPKTTEGRAIFDETNFVNTLLYM 748
BLAST of Calpain-B vs. Ensembl Cavefish
Match: zgc:55262 (calpain-1 catalytic subunit-like [Source:NCBI gene;Acc:103042634]) HSP 1 Score: 598.971 bits (1543), Expect = 0.000e+0 Identity = 305/643 (47.43%), Postives = 431/643 (67.03%), Query Frame = 1
Query: 421 MLKAK----NSLFEDPFFPANNTSLYYREAPP-----QKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIE-ADPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKS--LLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILD-VSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYI 2310
MLKA+ SLFED FPA+++SL + E P Q ++WKRP E+ FI DG +R D+ QG LGDCWLLAA++SL++ L+H+V+P Q F Y G F F+ WQFG W DVV+DDRLP G+L+++HS + EFWSAL+EKAYAK+ G YEAL GGS+SE EDFTGG+TEM++L AP +L++I+ + V R SL+GCSI+ +EA K L+KGHAYS+T V V + LVR+RNPWG EWTGA+SD S EW+ + R RL + E DGEFWM+F+DF++ F++LEIC+L+ ++ LE AG K KW C+ NGEW++ +AGGCRNF TF LNPQFKV + PD+ + + + ++ LMQK+ R +RKEG+D+ TIG+AIY++ +S L + +F AS A+S FINLREV R +LP G+Y+IVPSTF +EA F+LR+FSE+P ++++D+ D E+P E + +E + ++ F+ ++G+D EI ELQ IL+ + K ++ K +GF E CRSM+ +MDTD SG+LG EF VLW+ ++ + TVF++FD D+SGT +SYE+R AL GF+++N +F II RY + + FD+++
Sbjct: 39 MLKARCLQSRSLFEDSLFPASSSSLGFNELGPRSSKTQGVQWKRPPEICSRPQFIVDGATRTDICQGALGDCWLLAAIASLTLNDNLLHRVVPHGQSFGSGYAGIFHFQFWQFGEWMDVVIDDRLPVKDGKLLFVHSAEGGEFWSALVEKAYAKLNGCYEALSGGSTSEGFEDFTGGVTEMYEL-KKAPSDLYNIIQRAVDRGSLLGCSIDITSKFDMEAVTFKKLVKGHAYSVTAVHEVVYRGTPTKLVRIRNPWGE-VEWTGAWSDNSREWDSVDSSSRARLQVRSE-DGEFWMSFRDFLQEFSRLEICNLTADA-LE--AGQVK-KWSTCLYNGEWRRGSTAGGCRNFPATFWLNPQFKVNLTHPDKAGSSDC-SFLVALMQKDRRKKRKEGKDMETIGFAIYEVPGQYVGQSGVHLKRDFFLTNASSARSELFINLREVSSRFRLPAGEYIIVPSTFEPQKEADFVLRVFSEKPASAEELDD-------DVTAELPSENQLDESQIDAGFKSLFRQLAGSDMEISVTELQTILNRIISKHKDLKTDGFGPEACRSMINLMDTDGSGKLGLTEFHVLWEKIKRYLTVFRQFDLDKSGTMSSYEMRMALEAAGFKLTNHLFQLIILRYTEEDLTVDFDNFV 666
BLAST of Calpain-B vs. Ensembl Cavefish
Match: zgc:55262 (calpain-1 catalytic subunit-like [Source:NCBI gene;Acc:103042634]) HSP 1 Score: 561.607 bits (1446), Expect = 0.000e+0 Identity = 294/643 (45.72%), Postives = 419/643 (65.16%), Query Frame = 1
Query: 421 MLKAK----NSLFEDPFFPANNTSLYYREAPP-----QKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIE-ADPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILDVSFKRENFKFNGFSR---ETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYI 2310
MLKA+ SLFED FPA+++SL + E P Q ++WKRP E+ FI DG +R D+ QG LGDCWLLAA++SL++ L+H+V+P Q F Y G F F+ WQFG W DVV+DDRLP G+L+++HS + EFWSAL+EKAYAK+ G YEAL GGS+SE EDFTGG+TEM++L AP +L++I+ + V R SL+GCSI+ +EA K L+KGHAYS+T +V + LVR+RNPWG EWTGA+SD S EW+ + R RL + E DGEFWM+F+DF++ F++LEIC+L+ ++ LE AG K KW C+ NGEW++ +AGGCRNF TF LNPQFKV + PD+ + + + ++ LMQK+ R +RKEG+D+ TIG+AIY+ + L + +F AS A+S FINLREV R +LP G+Y+IVPSTF +EA F+LR+FSE+P ++++D+ D E+P E + +E + ++ F+ ++G+D EI ELQ IL+ + F + + + C + TD SG+LG EF VLW+ ++ + TVF++FD D+SGT +SYE+R AL GF+++N +F II RY + + FD+++
Sbjct: 39 MLKARCLQSRSLFEDSLFPASSSSLGFNELGPRSSKTQGVQWKRPPEICSRPQFIVDGATRTDICQGALGDCWLLAAIASLTLNDNLLHRVVPHGQSFGSGYAGIFHFQFWQFGEWMDVVIDDRLPVKDGKLLFVHSAEGGEFWSALVEKAYAKLNGCYEALSGGSTSEGFEDFTGGVTEMYEL-KKAPSDLYNIIQRAVDRGSLLGCSIDITSKFDMEAVTFKKLVKGHAYSVT---AVVYRGTPTKLVRIRNPWGE-VEWTGAWSDNSREWDSVDSSSRARLQVRSE-DGEFWMSFRDFLQEFSRLEICNLTADA-LE--AGQVK-KWSTCLYNGEWRRGSTAGGCRNFPATFWLNPQFKVNLTHPDKAGSSDC-SFLVALMQKDRRKKRKEGKDMETIGFAIYEYVGQS--GVHLKRDFFLTNASSARSELFINLREVSSRFRLPAGEYIIVPSTFEPQKEADFVLRVFSEKPASAEELDD-------DVTAELPSENQLDESQIDAGFKSLFRQLAGSDMEISVTELQTILNRIISKREFSDSLYCLIFFDRC------IQTDGSGKLGLTEFHVLWEKIKRYLTVFRQFDLDKSGTMSSYEMRMALEAAGFKLTNHLFQLIILRYTEEDLTVDFDNFV 655
BLAST of Calpain-B vs. Ensembl Cavefish
Match: si:dkeyp-50d11.2 (si:dkeyp-50d11.2 [Source:ZFIN;Acc:ZDB-GENE-091113-22]) HSP 1 Score: 555.058 bits (1429), Expect = 0.000e+0 Identity = 284/632 (44.94%), Postives = 407/632 (64.40%), Query Frame = 1
Query: 442 LFEDPFFPANNTSLYYREAPP-----QKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEADP-TKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKS--LLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILD-VSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYI 2310
LFED FPA +SL ++E P + + W RP E + FI DG +R D+ QG LGDCWLLAA++ L++ L+H+V+P Q F Y G F F+ WQFG W DVV+DDRLP G+L+++HS + EFWSAL+EKAYAK+ G YEAL GG +SE EDFTGG+TEM++L APPNL+ I+ + V R SL+GCSI+ +EA K L+KGHAYS+T V V + L+R+RNPWG EWTGA+SD+S EW+ I+ R RL E DGEFWM+F DF+K F++LEIC+L+ ++ ++ KW + GEW++ +AGGCRN+ TF +NPQ+K+ + DPD + ++ + ++ LMQK R R+EG+D+ TIG+AIY++ + L + +F S A+S FINLREV R LP G+Y+IVPSTF +E F+LR+FSE+ DS+++D+ E+ A ++P E E + + F ++G D EID +LQ IL+ V K ++ K +GF +E CR M+++MDT GRLG +F VLW+ ++ + T+F++FD D+SGT +SYE+R AL GF+++N +F II RYA + FD+++
Sbjct: 50 LFEDETFPAQPSSLGFKELAPGSAKTRGVRWMRPTEFCDDPHFIVDGATRTDICQGALGDCWLLAAIACLTLNEPLLHRVVPHGQSFHYQYAGIFHFQFWQFGDWVDVVIDDRLPVRDGKLLFVHSDEGAEFWSALVEKAYAKLNGCYEALSGGCTSEGFEDFTGGVTEMYEL-RKAPPNLFSIIRRAVERGSLLGCSIDITSFFDMEAVTFKKLVKGHAYSVTGVDEVEFRGNLTKLIRIRNPWGE-VEWTGAWSDESREWSGINPALRSRLHNRAE-DGEFWMSFSDFLKEFSRLEICNLTADALQSSEL----KKWNSSLYTGEWRRGSTAGGCRNYPATFWINPQYKIMLKDPD-TEGQDGCSFLVALMQKNRRQLRREGKDMETIGFAIYEVPLQFLGMAGVHLKRDFFLTNTSSARSELFINLREVSSRFCLPAGEYIIVPSTFEPQKEGDFVLRVFSEKAADSQELDD--ELTA-----DLPEEDALPESEIDEGFKALFAKLAGKDMEIDVPKLQMILNRVISKHKDLKTDGFGKEACRCMISLMDTSGCGRLGLTDFHVLWEKIKRYLTIFREFDLDKSGTMSSYEMRLALESAGFKLTNNLFQLIILRYAKPDLNVDFDNFV 666
BLAST of Calpain-B vs. Ensembl Cavefish
Match: capn1a (calpain-1 catalytic subunit-like [Source:NCBI gene;Acc:103024720]) HSP 1 Score: 546.969 bits (1408), Expect = 0.000e+0 Identity = 280/634 (44.16%), Postives = 421/634 (66.40%), Query Frame = 1
Query: 442 LFEDPFFPANNTSLYYREAPPQK-----IEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEADPT-KVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNEN--TGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKD--NAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILD-VSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYI 2310
LFEDP FPA SL ++E P +EW RP E+ + FI G +R D+ QG LGDCWLLAA++SL++ L+H+V+P Q F DY G F F+ WQFG W D+V+DDRLP G L+++HS + NEFWSAL+EKAYAK+ GSYEAL GGS++E EDFTGG++EM++L P +L+ I+ K + SL+GCSI+ T +EA K L+KGHAYS+T ++ VN + L+R+RNPWG EWTGA+SD S EW+EI ER+ L L E DGEFWM+F +F++ F++LEIC+L+P++ ++ S W +G W++ +AGGCRN TF +NPQ+K+ +++ D++ ++ + ++ L+QK+ R R++GQD+ TIG+A+Y++ + L K +F +S A+S TFINLREV R +LP G+Y+IVPSTF +EA F+LR+F+E+ +++++D+ EI A ++ E + ED + ++ F ++G D EI EL+ IL+ V + ++ K +GFS E+CR+MV +MD D S RLG EF++LW+ +R W ++++FD D+SGT +SYE+R A+ GF+++NR+ ++ RYA + +I FD++I
Sbjct: 48 LFEDPCFPAEPPSLGFKELGPHSSKTSGVEWMRPTELCDDPQFILGGATRTDICQGALGDCWLLAAIASLTLNEKLLHRVVPHGQSFQDDYAGIFHFQFWQFGEWVDIVIDDRLPVKDGELMFVHSAEGNEFWSALIEKAYAKLNGSYEALSGGSTTEGFEDFTGGVSEMYEL-RKPPRDLYRIISKALELGSLLGCSIDITSTFDMEAVTFKKLVKGHAYSVTGLRQVNFRGDLQRLIRIRNPWGQ-VEWTGAWSDNSSEWDEIDPTEREDLCLQME-DGEFWMSFNEFLRQFSRLEICNLTPDALSDDGL----SHWNTIQFHGSWRRGSTAGGCRNHPNTFWINPQYKITLLEEDDDPEDDEVACSFLVALLQKDRRRYRRQGQDMHTIGFAVYEIPEEFRGCQNVHLKKNFFLSHSSCARSETFINLREVSARLRLPPGEYLIVPSTFEPSKEADFVLRVFTEKQSETEELDD--EISA-----DLEDEEEITEDDIDDSFKSMFAQLAGEDMEISVHELRTILNRVVLRHKDLKTDGFSMESCRTMVNLMDKDGSARLGLVEFQILWNKIRKWLVIYRQFDLDKSGTMSSYEMRLAVESAGFKLNNRLNQILVARYAENE-VIDFDNFI 666
BLAST of Calpain-B vs. Ensembl Cavefish
Match: capn3b (calpain 3b [Source:ZFIN;Acc:ZDB-GENE-041001-149]) HSP 1 Score: 540.421 bits (1391), Expect = 0.000e+0 Identity = 284/659 (43.10%), Postives = 425/659 (64.49%), Query Frame = 1
Query: 358 SRFHVSKSLKESKDWFYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEA-DPTKVEAKAEKGLIKGHAYSITDVKSVNSKRG---RVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVV--DPDENDNENTG-TLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILD-VSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYI 2310
SR K K KD+ + + ++ K LF+DP FPA+++SLYY + P K+ WKRP E+ +N FI G +R D+ QG+LGDCWLLAA++ L++ L+++V+P+DQ F +Y G F F+ W++G W DVVVDDRLPT LV+ S + NEFWSALLEKAYAK+ G+YEALKGG++ EAMEDFTGG+TE +++ S AP +++IM K + R SLMGCSI++ P+ E K + GL++GHAYS+T V+ + G R+ LVR+R+PWG + S S +W+ ++ E+ + L + GEFWM F++F + FTKLEIC+L+P++ +++ KW++ + G W + SAGGCRN+ +TF NPQ+++++ D DE D+ G T+++ LMQK R R G L TIG+AIY+M N K L K +F TAS A+ ++INLREV R L G+YVIVPSTF+ E+ F+LR+FSE+ S+ ++ D + P+ + EE + + F+ I+G D +I+A EL+ + + + K + K GFS ++CRSM+A+MDTD +GRL EEFK LW ++ WK +F ++D D+S + +S+E+R A+ GF+++ ++ I RYA++ + FD YI
Sbjct: 52 SRNEAVKDAKRLKDFLELRDKYVR-KKVLFKDPLFPADDSSLYYNQKFPLKLVWKRPSEICKNPQFIVGGANRTDICQGDLGDCWLLAAIACLTLSETLLYRVVPADQSFSENYAGIFHFQFWRYGEWVDVVVDDRLPTSNNHLVFTRSGNKNEFWSALLEKAYAKLHGTYEALKGGNTLEAMEDFTGGVTEFYEI-SDAPREIYNIMKKALNRGSLMGCSIDSLVPSASETKTDTGLVRGHAYSVTAVEQGKQEDGSDTRIRLVRLRDPWGVA----PPPSRMSCDWSSLATSEQDQQRLKPVEAGEFWMGFEEFQQTFTKLEICNLTPDALQDDEL----LKWDVSVNEGRWVRGCSAGGCRNYPDTFWTNPQYRLRLTEEDDDEPDHGEKGCTVVVALMQKGRRRERCTGGTLLTIGFAIYEMHGN---KQHLPKDFFLYTASKARCKSYINLREVTERFCLSPGEYVIVPSTFDPNNESDFILRVFSEKTSTSEL---SGAVIEAD--PCVLPKSEDEESDEEKQFRAMFQQIAGDDMQINAKELRSVFNKLLTKYKGLKTEGFSLDSCRSMIALMDTDGTGRLNLEEFKHLWSKIQQWKLIFSRYDRDKSSSISSFEMRNAVADAGFQLNPQLHDIIAMRYASEHLYLDFDSYI 692
BLAST of Calpain-B vs. Ensembl Sea Lamprey
Match: capn3a (calpain 3a, (p94) [Source:ZFIN;Acc:ZDB-GENE-040912-97]) HSP 1 Score: 548.125 bits (1411), Expect = 0.000e+0 Identity = 304/765 (39.74%), Postives = 451/765 (58.95%), Query Frame = 1
Query: 403 FYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIE---------------------------------------------ADPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKV--VDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAY-DKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQ-----------------------------------------PYDSKQIDEKTEIVAPDWKKEIPPEVKREEDAN--NVKLQNAFKVISGADGEIDADELQQILD-VSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYIXXXXXXXXXXXXXKAQQKTSKGEAIFPQDEFMQCVLY 2421
F + + + + LFEDP FPA+N S+Y+ + P + +W RPKE+ + + + + + +G+LGDCW+LAA++ ++M L+ +VIP+ Q F+ +Y G F F+ W+FG W DVVVDDRLPT +LV++ S + NEFWSALLEKAYAKV GSYEALKGG+++EAMEDFTGG+TE F++ + P +L+ IM+K +R SLMGCSI+ A T E++ GL++ HAYSIT + V + V LVR+RNPWG+ EW G +SD S EWNEI+ E++RL L +DGEFW++FQDF + FTKLEIC+LSP++ +++ +KW + + G W + SAGGCRN+ +TF NPQ+++++ D D +DNE + I+ LMQK R R++G + TIG+AIY++ + + L K +F AS A+ ++INLREV R +LP G+YVIVPSTF +E +FLLR+F+E+ P +S+ D E P K PE +E D + + + FK ++G D E+ A +L IL+ V K ++ K +GFS E+CR+MVA+MDTD SG+LG+ +FK LWD L+ W+ +F+K+D D+SG+ SYE+R A+N G++++N+++ I R+A++ I FD++I L+RL +F FKA K G E++Q +Y
Sbjct: 53 FEELRTLCLSHDVLFEDPDFPAHNNSIYFSQNAPMQFQWMRPKELCVPRSIVIEA-NHTHICEGDLGDCWVLAAIACMTMNEELLARVIPAGQSFEENYAGIFHFQFWRFGEWVDVVVDDRLPTFNNQLVFVQSAERNEFWSALLEKAYAKVHGSYEALKGGNTTEAMEDFTGGVTEYFEM-KNPPKDLYKIMVKATSRRSLMGCSIDDGMCWRCGVPAPDSIGLVRSNMSPPILRHIQLTPIGIEDAKKRKIAMLTHAESRTPMGLVRNHAYSITGLDEVKHRSKIVQLVRLRNPWGH-VEWIGPWSDASKEWNEITPSEKQRLQLISAEDGEFWISFQDFCRNFTKLEICNLSPDALADDRT----NKWTVSVSEGRWVRGCSAGGCRNYPDTFWTNPQYRLQLYDEDDDPDDNELLCSFIVALMQKNRRKERRQGSTMLTIGFAIYEVTEELRGSREHLPKDFFLYNASRARCNSYINLREVTERFRLPPGEYVIVPSTFEAHQEGEFLLRVFAEKHNVSEEMDDIIGVKKKSKKKKNKPIVFISDRTKKGQQQPESPDESEPEDGNEEESEPKSNKLAEPENPKEPDLTPEDKQFKTIFKQLAGDDMEVGAADLHNILNKVITKHKDLKTSGFSLESCRTMVALMDTDLSGKLGYNDFKYLWDKLKFWQQIFRKYDSDKSGSMTSYEMRLAINEAGYQLNNQLYQIITLRHADENLNIGFDNFIACLIRLEAMFRAFKAFDKDGDGIIRLNVLEWLQLTMY 810
BLAST of Calpain-B vs. Ensembl Sea Lamprey
Match: capn9 (calpain 9 [Source:ZFIN;Acc:ZDB-GENE-010724-2]) HSP 1 Score: 541.576 bits (1394), Expect = 0.000e+0 Identity = 277/581 (47.68%), Postives = 388/581 (66.78%), Query Frame = 1
Query: 511 IEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMF--RLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIE-ADPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNEN---TGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEK--TEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILD-VSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIG 2226
++W +E+ QN FI DG +R D+ QG LGDCWLLAA++SL++ L+ +V+P Q+F Y G F F + W FG W DV +DDRLPT G+LV++HS D EFWSALLEKAY+K+ GSYEALKGGS +EAMEDFTGG+ E+ DL +APP+L+ IM K + S+MGCSI+ + ++ EA+ GL+KGHAYSIT V VN + +V L+R+RNPWG EW GA+SD +PEW + ER RL +DGEFWM+F DF+++FTK+EIC+L+P+ +E + + W + + +G W K +AGGCRNF ETF NPQF++K+ + D+ +E + I+ LMQK M RK+ +++ T+GYAIY++ D + K +F AS A+SP+FINLREV R +LP G+YVIVPSTF +EA FL+R+F+E+P SK+ D+K EI D K P + E+D K + F ++G D EI ELQ +L+ ++ K + GFS CRS+VA+ D D SG+LG +EF++LW+ ++ W+ +F+K DED SGT +SYE++ ALN G
Sbjct: 2 VKWGLSREIVQNPEFIVDGATRTDIKQGALGDCWLLAAIASLTLNDQLLAKVVPPGQNFRDGYAGIFHFQQKFWHFGEWMDVFIDDRLPTKNGQLVFVHSADKREFWSALLEKAYSKLNGSYEALKGGSIAEAMEDFTGGVYELLDL-KTAPPDLFRIMCKARQKGSMMGCSIDVTNASESEARTSNGLVKGHAYSITGVDEVNYRGQKVKLLRIRNPWG-EVEWNGAWSDHAPEWKALDQGERNRLQQVMAEDGEFWMSFDDFMRHFTKMEICNLTPD-RVEREGDPR--YWTVTVHDGRWLKGCTAGGCRNFPETFWTNPQFRLKLHNEDDEADETGSKACSFIVALMQKNM---RKQQRNMLTMGYAIYKIPDQT---GPMPKEFFLYNASKARSPSFINLREVSQRFRLPAGEYVIVPSTFEPHQEADFLVRVFAEKPNRSKESDDKIGAEIDLNDVAK---PGTETEDDR---KFKTLFGRLAGEDMEISPKELQDMLNKITAKHGELESTGFSMHACRSIVALTDVDGSGKLGLQEFRILWNKIKKWQAIFRKHDEDLSGTMSSYEMKNALNAAG 565
BLAST of Calpain-B vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003998.1 (pep scaffold:Pmarinus_7.0:GL501031:16584:50555:1 gene:ENSPMAG00000003440.1 transcript:ENSPMAT00000003998.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 496.123 bits (1276), Expect = 6.232e-165 Identity = 298/737 (40.43%), Postives = 420/737 (56.99%), Query Frame = 1
Query: 520 KRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEADPT-------------KVEAK-----------AEKGLIK--GHA-----------------------------YSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNEN--TGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDK-SLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQ-----------------------------------PYDSKQIDEK---------TEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILD-VSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYIXXXXXXXXXXXXXKAQQKTSKGEAIFPQDEFMQCVLY 2421
+ P+E+ N FI +G +R D+ QG LGDCWLLAA++SL+++P L+ +V+P Q F Y G F F+ WQFG W DVV+DD LPT +L +LHS D NEFWSALLEKAYAK+ GSYEALKGGS +EAMEDFTGG+ E ++L APP+L+ IM KG+ +AS+MGCSI+ T K+ A AE+ L GHA YSIT + V + V LVR+RNPWG EW GA+SD S EW + ER+RL +DGEFWM+F DF + F+K+EIC+LSP++ L ++G + W + G W + +AGGCRN+ TF NPQF +++ + D++ +++ + I+ LMQK MR +++ DL TIGYAIY++ A + L +F +S A+S FINLREV R +LP G YVIVPST+ +EA FLLR++ E+ P + +DE +E V + +P EV E + + FK ++G D EI ELQ +L+ V+ K + FK +GFS C+SMVA+MD+D SG+LG EFK+LW+ +++W+ +F+K+DED+SGT +SYE+R ALN GF ++N+++ I RYA++ I F+DYI +VRL +F F A K +G ++Q +Y
Sbjct: 1 RAPQEICYNPQFIVNGATRTDIKQGALGDCWLLAAIASLTLHPRLLARVVPEGQSFSDGYAGIFHFQFWQFGEWVDVVIDDLLPTQNNKLAFLHSTDRNEFWSALLEKAYAKLNGSYEALKGGSIAEAMEDFTGGVFEFYNL-KKAPPDLYRIMRKGLEKASMMGCSIDEGSTFKYRSTPRAINMSKMTATFRAWSSGIRRFAERFLPSRLGHARLLASSGIEITSMFDLEARLPNGLVKGHAYSITGLDQVFFEGQDVRLVRIRNPWGE-VEWNGAWSDNSWEWRVVDVSERRRLEQVRAEDGEFWMSFSDFKRNFSKMEICNLSPDT-LRTQSGSR--HWTVTAHEGRWLRGCTAGGCRNYAGTFWTNPQFHLRLHEEDDDPDDDEVACSFIVALMQKNMRRQKR---DLLTIGYAIYEIPLKAPPGCTHLPGEFFVRNSSRARSHNFINLREVSDRFRLPPGHYVIVPSTYEPHQEADFLLRVYCEKMNRSRGETKHNSVSKGRPIFRGAFKQVSNLGGCGGPNCYRVVDEHIADEILKSNSERVGEAVQPSMPVEVV--ETDEDRRFTQVFKKLAGEDMEISPRELQDLLNKVTAKLKVFKSDGFSLYACQSMVALMDSDGSGKLGLPEFKLLWEKIKMWQAIFRKYDEDRSGTMSSYEMRAALNAAGFHLNNQMYQLITMRYADESMTIKFNDYITCMVRLEAMFRIFHALDKDKRGVIRLGLGLWLQLTMY 727
BLAST of Calpain-B vs. Ensembl Sea Lamprey
Match: capn1a (calpain 1, (mu/I) large subunit a [Source:ZFIN;Acc:ZDB-GENE-040426-1263]) HSP 1 Score: 445.662 bits (1145), Expect = 8.175e-146 Identity = 255/657 (38.81%), Postives = 377/657 (57.38%), Query Frame = 1
Query: 376 KSLKESKDWFYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPP------QKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIE--ADPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCR--NFIETFHLNPQFKVKVVDPDENDNENTGT-LIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILDVSFKR-ENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYI 2310
++ K F K+ + LFEDP FPA+ +SL+ R P IEWKRP E+ ++ FI G +R D+ QG L DCWLL+A++SL++YP L+ +V+P Q F DY G F+FR Q+G W DV VDDRLPT +G LV++ S NEFWSALLEKAYAK+ G YEAL G EA+ DFTGG+ E F + ++AP + WD + + + + +++ C IE A + E GL++GHAYSIT V + V L+RVRNP+G E+TG +SD S WNE+ + ++KRL + E DGEFW++ +DF K F +EICH E E+E+ +K+ W + N W K ++AGG R + F LN QF + +++ D + G ++ L QK R RK G D IG IY+ L + + +SP FI REV R LP G Y+I+P T + G+EA+FLLRIF E+ + + + E V P + + + P A N ++ +F ++G D I AD+LQ ++ + ++ + FS E+CR +VA+ D D SG+LG+EEF ++W+ +++ +K D D+SGT +S E+ AL H F+V + I+ R++ I+FD ++
Sbjct: 21 RNFKTKSKTFQEIKKECLQRKILFEDPDFPASLSSLHIRVQPTVITQSNNFIEWKRPWELVRHPEFIVSGATRADINQGTLPDCWLLSAIASLTLYPDLLAKVVPMGQSFHEDYAGIFLFRFCQYGEWVDVHVDDRLPTKKGELVFVQSGTGNEFWSALLEKAYAKLNGCYEALAYGFPVEALCDFTGGVGETFTM-NNAPRDYWDGISRAIKKGAIICCGIETLAASGRGEKVTPMGLVEGHAYSITGVIKLKFPGKTVQLIRVRNPYGK-VEYTGPWSDGSTTWNEVPEEDQKRLHVRAE-DGEFWISDKDFRKNFHLVEICHQGSEVEIEH----RKNLWICELHNSMWLKGLNAGGSRRHRVFKEFPLNYQFFLTLLEEDNVMDVCHGCHFLLSLTQKHRRKFRKIGIDFVPIGIYIYKA---VVPGKKLTPQQLRKEKPLVESPEFIARREVTMRMTLPPGNYIIIPCTESAGQEAQFLLRIFMEKAQQHQGLASQLE-VTPYFPRMVSP-----PPAENFDMEESFLQVAGKDKLIKADKLQALITLEIQKYTTVVVDKFSMESCRVLVALFDIDNSGQLGYEEFLLVWEFIKMTLKTYKMCDVDKSGTLSSTELPMALEHSRFKVGKLMQRLIMMRFSEPNLTISFDSFV 661
BLAST of Calpain-B vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007888.1 (pep scaffold:Pmarinus_7.0:GL477427:174304:193458:1 gene:ENSPMAG00000007039.1 transcript:ENSPMAT00000007888.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 374.785 bits (961), Expect = 2.383e-120 Identity = 212/530 (40.00%), Postives = 301/530 (56.79%), Query Frame = 1
Query: 853 KVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIE-ADPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVV--DPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDN--AYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPD--------WKKEIPPE----------------VKREEDANNV-----------KLQNAFKVISGADGEIDADELQQILD--VSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIIT--FDDYI 2310
++ GSYEAL GGS++E EDFTGG+ E +L + P NLW ++ + R SLMGCSI EA + L+KGHAYS+T V+ + V LVR+RNPWG EWTGA+SD +P+W +SD +R RL ++ ++DGEFWM+F+DF++ F++LEIC+L+P++ ++ G W G W + SAGGCRNFI +F +K D D +DNE T I+ LMQKE R +R++G D+ T+GYAIY++ + + L K YFQ +S A+S FINLREV R LP G+Y++VPSTF +EA F+LRIFSE+ + T +P IP + R DA + F ++G D EI A ELQ IL+ VS +GF E CRSMV++MD D + +LG +EF LW ++ W+ +F ++D D +GT NS E+R AL GFR++ ++ + TRYA+ + IT FD+Y+
Sbjct: 1 RLQGSYEALSGGSTTEGFEDFTGGVAESIEL-NKPPSNLWRMLQQATLRGSLMGCSINITSQADSEAITSRKLVKGHAYSLTGTTEVDYQGQMVRLVRIRNPWGQ-VEWTGAWSDNAPQWGYVSDEDRGRLQVS-QEDGEFWMSFEDFLRQFSRLEICNLTPDALDDDSLG----HWSANNFEGSWVRGCSAGGCRNFIVHLWEKWKFHLKHTEEDDDPHDNEAGCTFIVALMQKERRKKRRQGGDVLTVGYAIYEVPEQFVGQPQVKLKKDYFQYHSSKARSEVFINLREVSQRFTLPAGEYIVVPSTFEPNQEADFILRIFSEKQSVTVSARTTTSNTSPHSPTHTCTYTNTRIPHDGCSHTRERAHSRARTPTPRHSDAPTSFTSFKFSSTCPRFYKLFDRLAGEDQEISAKELQSILNRVVSKLHSTVTTDGFGLEACRSMVSLMDKDGTAKLGVKEFNFLWKKIKNWQQIFLEYDTDSTGTMNSMEMRLALEAAGFRLNEKLNQIVTTRYADGENGITIDFDNYV 523
BLAST of Calpain-B vs. Ensembl Nematostella
Match: EDO40193 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S7W7]) HSP 1 Score: 618.616 bits (1594), Expect = 0.000e+0 Identity = 314/673 (46.66%), Postives = 446/673 (66.27%), Query Frame = 1
Query: 415 KRMLKAKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSD---QDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEADPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGR----VNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREE-DANNVKLQNAFKVISGADGEIDADELQQILDVSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYIXXXXXXXXXXXXXKAQQKTSKGEAIFPQDEFMQ 2409
+R +++ FED F A ++S+++ + PP+ EWKRP ++ ++ G SR D+ QG LGDCWLLAA+++L+ + ALM++V+P D DR+Y GA F +WQ+G W DV++DDRLPT G+LV++HS + NEFWSALLEKAYAK+ GSYE+LKGG + EA+EDFTGG+ E FDL + P +L+ IM R +LMGCSI A ++EAK GL+KGHAY++T + V +K R V LVRVRNPWGN EW GA+ D S EW +SD E+K +GLTFEDDGEFWM+F DF K+FT LEIC L +S +E + +E E+G W++ ++AGGCRNFI+TFH NPQF++ + ++D ++ T+II LMQK+ R +K G + TIG++IY++++ + LD +FQ AS A+SP FIN REV GR+ LP G+YV++P+TFN EE F+LR++SE+ + + ++DEKTE AP ++ + ++ RE+ D N K + F +SG DGEIDA ELQQIL + K + FS E RS+++M D D SG+LGFEEFK W ++ W +F+ FDED+SG ++YE+R AL GF +SN V A+ RY+ G + DD++ +L RL + F+ Q A F D+F++
Sbjct: 10 RRQVRSTGKPFEDDEFLAIDSSIFFSKRPPRPFEWKRPSDICEDPHMFVGGASRLDIQQGMLGDCWLLAAIAALTQHSALMNKVVPQDYVANPNDRNYTGALKFCIWQYGEWKDVIIDDRLPTYNGKLVFVHSSERNEFWSALLEKAYAKLNGSYESLKGGQTGEALEDFTGGLAESFDLNKNVPKDLFCIMQTAEKRHALMGCSIAAKANEIEAKLNNGLVKGHAYTVTGCRQVKAKTRRGDLDVELVRVRNPWGNEREWKGAWGDGSSEWQLLSDYEKKSIGLTFEDDGEFWMSFDDFKKHFTTLEICMLGADSAMEGSS----VGFESRSEHGMWRRGLTAGGCRNFIKTFHKNPQFRI-TLQDSDDDADDLCTVIIALMQKDRRKEKKHGINPLTIGFSIYKLEEEKDKQGNLDMEFFQYHASTARSPVFINSREVVGRYALPPGEYVVIPTTFNPNEEGNFVLRMYSEKAHKTCEVDEKTEF-APQHQRRV--QISREQHDTLNSKFKEFFDNLSGRDGEIDAKELQQILSTALK-NDLGGKPFSLEGARSIISMYDEDASGKLGFEEFKETWLQVKKWMKIFQVFDEDKSGEMDTYELRGALKEAGFTLSNSVLYAVSARYSTGDGKVNVDDFMEILTRLNILSAAFQKQAGQGGRRAAFDIDQFLE 673
BLAST of Calpain-B vs. Ensembl Nematostella
Match: EDO48633 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJ83]) HSP 1 Score: 574.704 bits (1480), Expect = 0.000e+0 Identity = 300/657 (45.66%), Postives = 425/657 (64.69%), Query Frame = 1
Query: 370 VSKSLKESKDWFYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDR-DYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEA---DPTKVEAKAEKGLIKGHAYSITDVK--SVNSKRGR--VNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREEDANNVKLQ--NAFKVISGADGEIDADELQQILDVSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYI 2310
+S+ ++ F + +R L LFED FPA+N L+ R Q I WKRP E+ ++ FIA G S DV QG + +CW +AA++ L+ L+H V P DQ F+ Y G F F WQFG W +++VDD LPT GRL + S D +EFWSALLEKAYAK+ GSYEA+ G SEA++D TGG+ E DL + P L+++MMK R SLMGCSIE + E E GLI HAYSIT ++ +++S RGR V+L+R+RNP NS EW GA+SD+SPEWN +S E++ L LTF+DDGEFWM+F DF+ FT+LEIC L+P+S +N + KW++ E G WQK V+AGGCRNFI +F+ NPQF+V + +PD++D+E T+I+ LM+ + S R + D IG+AIY+ + LD+ +F+ + A + +F LRE+ R++L G+YV++PSTFN G+E FLLRIFSE+P + +ID++T++ + P V ++DA ++ + + F ++G DGE+D ELQ+IL SF RE N F+ E CRSM++M DTD+SG LG+ EF+ L L WK F K+D D SG N+YE+R ALN +G+++SN ++++ RY NK+G I+FD +I
Sbjct: 1 MSRRALHKRERFASLQRQLLNSKRLFEDRDFPASNRVLFTRRRSVQLI-WKRPHEISKSPQFIARGFSSSDVAQGRIRNCWFVAALADLTTNIDLLHHVCPPDQSFEPGSYCGMFRFNFWQFGEWVEIIVDDYLPTYNGRLYFTKSDDPDEFWSALLEKAYAKLCGSYEAMSLGRISEALQDMTGGMIENVDL-QNPPHGLFNVMMKAYERQSLMGCSIEVKKYSSDQYEGTLENGLITRHAYSITGLRKATLSSLRGRPEVDLIRLRNPHANSSEWRGAWSDRSPEWNMLSSQEKRELNLTFDDDGEFWMSFDDFLNNFTRLEICMLTPDSMEQN----DRRKWDVQNEKGRWQKGVTAGGCRNFIASFYTNPQFRVCLKNPDDDDDE-LCTVIVSLMRVD-HSHRVKIPDRAPIGFAIYKAPSDMRKGDRLDRAFFEYNKAVAMTTSFSKLREISSRYRLHAGEYVVIPSTFNPGQEGDFLLRIFSERPQYASEIDQETKMTSG------PKVVITQDDAKSIDHEFWDVFHQMAGEDGEVDQYELQEILSKSFTRE-LNNNLFALEACRSMISMFDTDRSGALGYNEFRALMILLEKWKNHFHKYDRDHSGDMNAYELRDALNGLGYQLSNSAMSSLVLRYHNKRGTISFDTFI 642
BLAST of Calpain-B vs. Ensembl Nematostella
Match: EDO34860 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SN59]) HSP 1 Score: 354.369 bits (908), Expect = 3.493e-111 Identity = 204/524 (38.93%), Postives = 302/524 (57.63%), Query Frame = 1
Query: 400 WFYNQK-RMLKAK----NSLFEDPFFPANNTSLYY-REAPPQKIEWKRPKEMQQNVA--FIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSD--QDFD----RDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPP-------NLWDIMMKGVTRASLMGCSIEADP-TKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNL--------VRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKK--SKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKS--PTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDW 1869
+F NQ+ LKA+ LF D F +SLYY R PP+ +EWKRP E+ DG DV QG+LG+CW +AA SSL++ P L+++VIP Q++D +Y G F FR ++ G W +VVVDD LPTV G+LVY+HSK+ NEFWSAL+EKAYAK+AG+YEAL GS+++A+ DFTGG+ E L + L+ + +G SLM SI+ + ++E + + GLIKGHAY +T VK V G ++L +R+RNPWG EW G +SD+S EW ++ +RK++G+ FE+DGEFWM F DF + FT +CH+ NKA I K +W + N W + +AGGC +TF NPQ+ + D N+ E+ ++ LMQ++ R + +G TIG+ I ++++N R +++ + F K+ FIN REV R L G+Y++VPST+ F++RIF+ + ++ +D+ + + W
Sbjct: 6 YFANQRYSKLKAECEKNEQLFVDSVFKPETSSLYYSRSCPPEPVEWKRPSEICHPAVPHLFVDGADSHDVTQGKLGNCWFVAACSSLALRPFLLYKVIPDKDKQEWDPKKPENYQGIFRFRFYRQGEWTEVVVDDLLPTVNGKLVYIHSKEKNEFWSALIEKAYAKLAGTYEALAAGSTADALVDFTGGVAEAISLSNGGYAEDQEKRLQLFKTLERGHKEKSLMSASIKINNRNEMEQRLDTGLIKGHAYGVTMVKKVTVGEGLLSLFNKKHFYMIRLRNPWGEK-EWNGRWSDESEEWKKLKPGDRKKMGIVFENDGEFWMEFDDFCREFTDCTLCHVI------NKAMIHKVFKQWHIYKHNYNWTQGQNAGGCVQNQDTFLKNPQYAFSITD---NEGEDA---MVSLMQEDRRKDKSKGVGNLTIGFYIMKVEEN---------RKYRVHSMFPKAGDSIFINAREVMNRFFLAAGRYLVVPSTYEPHTPGHFMMRIFTRKRSNAMFLDQDHSVGSKIW 507
BLAST of Calpain-B vs. Ensembl Nematostella
Match: EDO48536 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJ84]) HSP 1 Score: 328.946 bits (842), Expect = 8.046e-102 Identity = 192/506 (37.94%), Postives = 265/506 (52.37%), Query Frame = 1
Query: 529 KEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSD----QDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEADPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQI--------------TASFA--KSPTFINLREVCG---------------------------------------RHKLPIGKYVIVP-------STFNQGEEAKFLLRIFSEQPYDSKQIDEKT 1848
+E+ F DG SR D+ QG LG+CWLLAA+S L+ + L+ +++P F Y G +F+LWQ+G W V++DDRLPT +G+LV++HS + NEFW ALLEKAYAK+ GSYEALKGG + EA+EDFTGG++E+F L AP NL+ IM + R++LMGCSI S RG V L+R+RNPWG+ EWTGA+ D+SPEW E+S E++ L L F+ DGEFWM+F+DF +F KLE+C L E+ ++ S+ +ENG W+K+VSAGGCRNF+ETFH NPQF++ E TG + + + M EG IG+A+Y++ + K L + F + FA K + R +CG + +G++ + P STF Q EA FLLRIFSE+ S + E T
Sbjct: 11 QEICSEPRFYVDGASRLDIKQGVLGNCWLLAAMSVLAQHKPLVDRIMPEGVYEADRFSPRYTGKVVFKLWQYGEWVTVIIDDRLPTYKGKLVFVHSPEKNEFWCALLEKAYAKIHGSYEALKGGLTGEALEDFTGGLSEVFVL-EDAPANLFQIMQRAEKRSALMGCSINV--------------------------FLSTRGGVELIRIRNPWGDEKEWTGAWGDRSPEWQELSQDEKRSLDLEFDVDGEFWMSFEDFKSHFDKLEVCMLDVENAIDKTQISFNSR----LENGFWRKHVSAGGCRNFLETFHTNPQFRLVSRVMSRMICEGTGFALYKVSNRVMSREICEG-----IGFALYKVSNRVISKVLCEGTGFALYKVSNRVISRVICEGTGFALYKVSNRVISRVICGGIGFALYKIDDITKRKYGVSRLDKEYFQFNKSTERSTFINSREVVGRFELAPGEYVIVPSTFIQNAEADFLLRIFSEKAVSSSALTEST 480
BLAST of Calpain-B vs. Ensembl Nematostella
Match: EDO37991 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SE78]) HSP 1 Score: 152.91 bits (385), Expect = 1.049e-38 Identity = 110/327 (33.64%), Postives = 164/327 (50.15%), Query Frame = 1
Query: 427 KAKNSLFEDPFFPANNTSLYY---REAPPQKIEWKRPKEM------QQNVAFIADGLSR-FDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPT-VRGRLVY--LHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDL-------GSSAPPN---LWDIMMKGVTRASLMGCSIEAD--PTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWN-EISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHL 1329
K + F D FP SLY P+ +W RP E+ + N+ + R D++QG LGDCW L++++ L+ P L+ +++ + + + GA+ RL + G W +++DD LP RG+ VY H + L W L+EKA AK+ GSYEAL G S E + TG E L G++ P + +W ++ T LMG S D P + GL HAYS+ DV+ V+ R L+R+RNPWG WTG +SD S W E+ R L + +G FWM+ +DF+KYF +++C +
Sbjct: 38 KTQEIQFVDDSFPPVLKSLYINPRHSEHPRVADWLRPNEIDNFSTCRTNIPWAVFRNPRPSDIIQGVLGDCWFLSSLAVLAERPELLEKILITREVSPQ---GAYQVRLCKDGKWTVILIDDLLPCNQRGQPVYSWAHRRQL---WVPLIEKAMAKLHGSYEALTAGRSIEGLATLTGAPCESIALHESQNNSGNTEPIDKDLIWAQLISSRTAGFLMGASCGGDTQPEEEPGYNAVGLQTHHAYSVLDVRDVHGIR----LLRLRNPWGQY-SWTGDWSDASSLWTPEL----RHELMVHGAQEGVFWMSLEDFMKYFDSVDVCKI 349
BLAST of Calpain-B vs. Ensembl Medaka
Match: zgc:55262 (calpain-1 catalytic subunit [Source:NCBI gene;Acc:101155642]) HSP 1 Score: 573.933 bits (1478), Expect = 0.000e+0 Identity = 291/632 (46.04%), Postives = 419/632 (66.30%), Query Frame = 1
Query: 442 LFEDPFFPANNTSLYYREAPPQK-----IEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIE-ADPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKS--LLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILD-VSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYI 2310
LFED FP +SL + E P+ + W RP E+ + FI DG +R D+ QG LGDCWLLAA++SL++ L+H+V+P Q F + Y G F F+ WQFG W DVV+DDRLP G+L+++HS + EFWSALLEKAYAK+ G YEAL GGS+SE EDFTGG+TEM+DL PP+L+ I+ + V R SL+GCSI+ T EA K L+KGHAYS+T V V + LVR+RNPWG EWTG +SD S EW+ + R RL +DGEFWM F DF++ F+++EIC+L+ ++ L+N + KW + GEW++ +AGGCRN+ TF LNPQFK+ + PD + + + ++GLMQK+ R +R+EG+D+ TIG+A+Y++ D S L + +F AS A+S FINLREV R +LP G+Y++VPSTF +EA F+LR+FSE+P DS+++D+ ++VA ++P E+ +E + ++ FK ++G D EI ELQ IL+ + K ++ K +GF+ E CRSM+ +MDTD SG+LG +EF VLW+ ++ + T+F++FD D+SGT +SYE+R AL GF+++N +F II RY + + FD+++
Sbjct: 51 LFEDNLFPCAASSLGFNELGPRSSKTYGVRWMRPTELCKRPEFIVDGATRTDICQGALGDCWLLAAIASLTLNDNLLHRVVPHGQCFKQGYAGIFHFQFWQFGEWVDVVIDDRLPAKDGKLLFVHSVEGTEFWSALLEKAYAKLNGCYEALSGGSTSEGFEDFTGGVTEMYDL-IKPPPDLFGIISRAVERGSLLGCSIDITSSTDREAVTFKKLVKGHAYSLTGVDEVMYRGNLTRLVRMRNPWGE-MEWTGPWSDDSKEWSYVDSSVRDRLQ-NRSEDGEFWMAFSDFLREFSRVEICNLTADA-LDN---TQMKKWSSSLFQGEWRRGSTAGGCRNYPATFWLNPQFKISLQHPDVSGKPDC-SFLVGLMQKDRRKKRQEGKDMETIGFALYEVPDEFKGSSNVHLKRDFFLTHASSARSELFINLREVSSRLRLPAGEYIVVPSTFEPNKEADFVLRVFSEKPADSEELDD--DVVA-----DLPKEILLDESQIDAGFKSLFKQLAGPDMEISVTELQNILNKIISKHKDLKTDGFTPEACRSMINLMDTDGSGKLGLKEFHVLWEKIKRYLTIFRQFDLDKSGTMSSYEMRIALESAGFKLTNHLFQLIILRYTEEDMSVDFDNFV 667
BLAST of Calpain-B vs. Ensembl Medaka
Match: capn3b (calpain-3 [Source:NCBI gene;Acc:101169329]) HSP 1 Score: 560.836 bits (1444), Expect = 0.000e+0 Identity = 298/694 (42.94%), Postives = 429/694 (61.82%), Query Frame = 1
Query: 322 DPKQTDSESIYGSRFHVSKSLKESKDW--FYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEADPTK-VEAKAEKGLIKGHAYSITDVKSVN--SKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENT-GTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQM-KDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAP--------DWKKEIPPEVKR---------------EEDANNVKLQNAFKVISGADGEIDADELQQIL-DVSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYI 2310
D T + SIY + ++++K++K F + K LFEDP FPAN++SL+Y P KIEWKRP E+ +N FI DG +R D+ QGELGDCWLLAA++ L++ L+++VIP +Q F +Y G F F+ W++G W D+VVDDR+PT LV+ S NEFWSALLEKAYAK+ GSYEALKGG++ +AMEDFTGG+TE+F+L S AP L+ IM K + R SLMGCSI+ +E++ +GL++GHAYSI ++ + S+ G++ L+R+RNPWG W G +S S EW+ +S +R L + EFWM+F DF K FTKLE+C+L+P++ G ++ W + I G W + SAGGCRNF +TF NPQ+++++ + D+ + E T+++ LMQK R +R G TIG++IY++ K+ L K +F AS AK +INLREV R +LP G+YVI+P+TF EE +FLLR+FSE S++ + + + P KEI E R EE + + ++ I+G D EI A EL I+ +V K K +GFS ETCRSM+A+MDTD SG+L +EFK LW ++ W+ +FK++D+D + +S+E+R A+N GF ++ +++ I RYA+K I FD YI
Sbjct: 35 DYSLTGANSIYSAILSRNEAVKDAKRLKTFLELRDKYVKKKVLFEDPLFPANDSSLFYSHKPSMKIEWKRPTEICENPQFIIDGANRTDICQGELGDCWLLAAIACLTLSEKLLYRVIPPEQSFTENYAGIFHFQFWRYGEWIDIVVDDRIPTCNNLLVFTKSFRENEFWSALLEKAYAKLHGSYEALKGGNTLDAMEDFTGGVTELFEL-SEAPKGLFIIMKKALERGSLMGCSIDVTTANDIESRTNQGLVRGHAYSIIALEECDQVSEDGKIRLIRLRNPWGWVL-WKGPWSVNSKEWSTVSTADRDNLKKQTLEMSEFWMSFDDFKKNFTKLEMCNLTPDA----LTGDERQSWTVSINEGRWVRGSSAGGCRNFPDTFWTNPQYRLRLYEEDDPEEEQVLCTVVVALMQKGRRMQRHAGAKFLTIGFSIYEVPKELQGQNQHLQKDFFLYNASKAKCKAYINLREVTERFRLPPGEYVILPTTFKAHEEGEFLLRVFSENKSTSEEAETSIDKIEPIVFVSDRARTNKEIEHEGIRGEKKKKPKRKPLQAVEETEEEKQFRVIYQKIAGEDMEICASELMAIMKNVLSKHSEIKSDGFSLETCRSMIALMDTDGSGKLNLQEFKHLWKKIKEWQLIFKRYDKDGKCSISSFEMRNAVNDAGFNLNKQLYDIIAMRYADKHLNIDFDSYI 722
BLAST of Calpain-B vs. Ensembl Medaka
Match: zgc:55262 (calpain-1 catalytic subunit [Source:NCBI gene;Acc:101155642]) HSP 1 Score: 548.51 bits (1412), Expect = 0.000e+0 Identity = 286/632 (45.25%), Postives = 402/632 (63.61%), Query Frame = 1
Query: 442 LFEDPFFPANNTSLYYREAPPQK-----IEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIE-ADPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKS--LLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILD-VSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYI 2310
LFED FP +SL + E P+ + W RP E+ + FI DG +R D+ QG LGDCWLLAA++SL++ L+H+V+P Q F + Y G F F+ WQFG W DVV+DDRLP G+L+++HS + EFWSALLEKAYAK+ G YEAL GGS+SE EDFTGG+TEM+DL PP+L+ I+ + V R SL+GCSI+ T EA K L+KGHAYS+T V V + LVR+RNPWG EWTG +SD S EW+ + R RL +DGEFWM F DF++ F+++EIC+L+ ++ L+N + KW + GEW++ +AGGCRN+ TF LNPQFK+ + PD + + + ++GLMQK+ R +R+EG+D+ TIG+A+Y++ D S L + +F AS A+S FINLREV R +LP G+Y++VPSTF +EA F+LR+FSE+P DS + +IV R D + D EI ELQ IL+ + K ++ K +GF+ E CRSM+ +MDTD SG+LG +EF VLW+ ++ + T+F++FD D+SGT +SYE+R AL GF+++N +F II RY + + FD+++
Sbjct: 51 LFEDNLFPCAASSLGFNELGPRSSKTYGVRWMRPTELCKRPEFIVDGATRTDICQGALGDCWLLAAIASLTLNDNLLHRVVPHGQCFKQGYAGIFHFQFWQFGEWVDVVIDDRLPAKDGKLLFVHSVEGTEFWSALLEKAYAKLNGCYEALSGGSTSEGFEDFTGGVTEMYDL-IKPPPDLFGIISRAVERGSLLGCSIDITSSTDREAVTFKKLVKGHAYSLTGVDEVMYRGNLTRLVRMRNPWGEM-EWTGPWSDDSKEWSYVDSSVRDRLQ-NRSEDGEFWMAFSDFLREFSRVEICNLTADA-LDN---TQMKKWSSSLFQGEWRRGSTAGGCRNYPATFWLNPQFKISLQHPDVSGKPDC-SFLVGLMQKDRRKKRQEGKDMETIGFALYEVPDEFKGSSNVHLKRDFFLTHASSARSELFINLREVSSRLRLPAGEYIVVPSTFEPNKEADFVLRVFSEKPADSDCLCLLLDIV-------------RVTDL----------CLCQQDMEISVTELQNILNKIISKHKDLKTDGFTPEACRSMINLMDTDGSGKLGLKEFHVLWEKIKRYLTIFRQFDLDKSGTMSSYEMRIALESAGFKLTNHLFQLIILRYTEEDMSVDFDNFV 651
BLAST of Calpain-B vs. Ensembl Medaka
Match: capn3a (calpain-3 [Source:NCBI gene;Acc:101157263]) HSP 1 Score: 548.125 bits (1411), Expect = 0.000e+0 Identity = 295/657 (44.90%), Postives = 430/657 (65.45%), Query Frame = 1
Query: 370 VSKSLKESKDWFYNQKRMLKAKNSLFEDPFFPANNTSLYYREA-----PPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEA-DPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNEN--TGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIP-PEVKREEDANNVKLQNAFKVISGADGEIDADELQQILD-VSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYI 2310
V+K L + F ++ + LFED FPA SL ++E + I WKRPKE+ N FI G SR D+ QG+LGDCWLLAA++ L++ L+ +V+P +Q F Y G F F+ W++G W DVVVDDR+PT+ +LV+ S + NEFWSALLEKAYAK+ GSYEALKGG+++EAMEDFTGG+TE +++ AP L+ M K + R SLMGCSI++ P + E + GL+KGHAYS+T V K +V LVR+RNPWG EW G +SD S EW +S E+++L +DGEFWM+F+DF K +TK+EIC+L+P++ LE+ K KW + + G W + SAGGCRN+ +TF NPQ+++++++ D++ ++N T ++ LMQK R RK G +LFTIG++IY+M N K L K +F + +S A+S ++INLREV R +L G+YVIVPST+ +E +F+LR+FSE+ S++I+ + E P +P P EE + + F+ I+G D EI A++L+ +L+ V +R++ GFS E+CRSM+A+MD D +GRL +EF+ LW+ ++ W+ +FK ++ DQSG NSYE+R A+N GFR++N+++ I RYAN+ I FD +I
Sbjct: 26 VTKPLHFNGQDFSTLRQECLQRKGLFEDDSFPATVESLGFKELGHKSNKVKNIVWKRPKEICDNPQFIVGGASRTDICQGDLGDCWLLAAIACLTLNEKLLFRVVPQEQSFSESYAGIFHFQFWRYGDWVDVVVDDRIPTLNNQLVFTKSAERNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTEFYEM-KEAPKELYKTMKKALERGSLMGCSIDSLVPARFETRTTTGLVKGHAYSVTAVDE--QKESKVRLVRLRNPWG-QVEWNGPWSDNSKEWATLSKAEKEKLQHQSAEDGEFWMSFEDFKKNYTKIEICNLTPDT-LEDD---KIHKWTVSVNEGRWLRGCSAGGCRNYPDTFWTNPQYRLRLLEEDDDPDDNEVACTFVVSLMQKNRRRERKMGANLFTIGFSIYEMHGN---KQHLQKDFFLLNSSKARSKSYINLREVTQRFRLSPGEYVIVPSTYEPHQEGEFILRVFSEKRNTSEEIENRIEADHP-----VPAPASVGEESEEDHHFRTIFQEIAGEDMEITANKLKNVLNRVITERKDLNTVGFSLESCRSMIALMDMDGTGRLNLQEFRHLWNKIKQWEGIFKHYNADQSGIINSYEMRNAVNDAGFRLNNQLYHIITLRYANENMNIDFDSFI 666
BLAST of Calpain-B vs. Ensembl Medaka
Match: capn3a (calpain-3 [Source:NCBI gene;Acc:101157263]) HSP 1 Score: 546.969 bits (1408), Expect = 0.000e+0 Identity = 305/698 (43.70%), Postives = 450/698 (64.47%), Query Frame = 1
Query: 370 VSKSLKESKDWFYNQKRMLKAKNSLFEDPFFPANNTSLYYREA-----PPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEA-DPTKVEAKAEKGLIKGHAYSITDV---KSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNEN--TGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQM-KDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIP-PEVKREEDANNVKLQNAFKVISGADGEIDADELQQILD-VSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYIXXXXXXXXXXXXXKAQQKTSKGEAIFPQDEFMQCVLY 2421
V+K L + F ++ + LFED FPA SL ++E + I WKRPKE+ N FI G SR D+ QG+LGDCWLLAA++ L++ L+ +V+P +Q F Y G F F+ W++G W DVVVDDR+PT+ +LV+ S + NEFWSALLEKAYAK+ GSYEALKGG+++EAMEDFTGG+TE +++ AP L+ M K + R SLMGCSI++ P + E + GL+KGHAYS+T V + K +V LVR+RNPWG EW G +SD S EW +S E+++L +DGEFWM+F+DF K +TK+EIC+L+P++ LE+ K KW + + G W + SAGGCRN+ +TF NPQ+++++++ D++ ++N T ++ LMQK R RK G +LFTIG++IY++ K+ +K L K +F + +S A+S ++INLREV R +L G+YVIVPST+ +E +F+LR+FSE+ S++I+ + E P +P P EE + + F+ I+G D EI A++L+ +L+ V +R++ GFS E+CRSM+A+MD D +GRL +EF+ LW+ ++ W+ +FK ++ DQSG NSYE+R A+N GFR++N+++ I RYAN+ I FD +I LVRL +F F+A + G E++Q +Y
Sbjct: 26 VTKPLHFNGQDFSTLRQECLQRKGLFEDDSFPATVESLGFKELGHKSNKVKNIVWKRPKEICDNPQFIVGGASRTDICQGDLGDCWLLAAIACLTLNEKLLFRVVPQEQSFSESYAGIFHFQFWRYGDWVDVVVDDRIPTLNNQLVFTKSAERNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTEFYEM-KEAPKELYKTMKKALERGSLMGCSIDSLVPARFETRTTTGLVKGHAYSVTAVDECRPSQQKESKVRLVRLRNPWG-QVEWNGPWSDNSKEWATLSKAEKEKLQHQSAEDGEFWMSFEDFKKNYTKIEICNLTPDT-LEDD---KIHKWTVSVNEGRWLRGCSAGGCRNYPDTFWTNPQYRLRLLEEDDDPDDNEVACTFVVSLMQKNRRRERKMGANLFTIGFSIYEVPKEMHGNKQHLQKDFFLLNSSKARSKSYINLREVTQRFRLSPGEYVIVPSTYEPHQEGEFILRVFSEKRNTSEEIENRIEADHP-----VPAPASVGEESEEDHHFRTIFQEIAGEDMEITANKLKNVLNRVITERKDLNTVGFSLESCRSMIALMDMDGTGRLNLQEFRHLWNKIKQWEGIFKHYNADQSGIINSYEMRNAVNDAGFRLNNQLYHIITLRYANENMNIDFDSFISCLVRLEAMFRAFQAFDQDGDGTIRLSVLEWLQLTMY 712
BLAST of Calpain-B vs. Planmine SMEST
Match: SMESG000067761.1 (SMESG000067761.1) HSP 1 Score: 1477.61 bits (3824), Expect = 0.000e+0 Identity = 725/729 (99.45%), Postives = 728/729 (99.86%), Query Frame = 1
Query: 238 MPRFNDSSNTNESVGSRYSINRFTPLSPDPKQTDSESIYGSRFHVSKSLKESKDWFYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEADPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILDVSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYIXXXXXXXXXXXXXKAQQKTSKGEAIFPQDEFMQCVLYM 2424
MPRFNDSSNTNESVGSRYSINRFTPLSPDPKQTDSESIYGSRFHVSKSLKESKDWFYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALM+QVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEADPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILDVSFKR+ FKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYILLLVRLRTVFLT+KAQQKTSKGEAIFPQDEFMQCVLYM
Sbjct: 1 MPRFNDSSNTNESVGSRYSINRFTPLSPDPKQTDSESIYGSRFHVSKSLKESKDWFYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMYQVIPSDQDFDRDYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEADPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILDVSFKRD-FKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYILLLVRLRTVFLTYKAQQKTSKGEAIFPQDEFMQCVLYM 728
BLAST of Calpain-B vs. Planmine SMEST
Match: SMESG000039299.1 (SMESG000039299.1) HSP 1 Score: 836.254 bits (2159), Expect = 0.000e+0 Identity = 419/688 (60.90%), Postives = 535/688 (77.76%), Query Frame = 1
Query: 379 SLKESKDWFYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDR-DYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEADPTKVEAKAEKGLIKGHAYSITDVKSVN----SKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPES-ELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILDVSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYIXXXXXXXXXXXXXKAQQKTSKGEAIFPQDEFMQCVLYM 2424
S+ S+D + N K L+ K L+EDP FPA N SLY+ + PP+KI+WKRP E+ QN FI++G SRFDV QGELGDCWLLAAV+ LS++ L +QV+PSDQ+F + Y G F FR W+FG W +V+VDDRLPT +LVY+HS D NEFWSALLEKAYAK++GSYE+LKGGS+SEA+EDFTGG+TE FD+ P NL+DIM K +R SLMGCSIEADP K EA+ GLI GHAYSITDVK VN K G++ L+R+RNPWGN EW G++SDKSPEWN IS +R +GLTF+DDGEFWM++QDF+K F K+EICHL P+S E + G KKS+WEMCIENGEW+K ++AGGCRNF++TF NPQF+VKVVDPDE+D+EN GT+IIGLMQK+MR RRKEG + +TIGYAIYQ+KD ++ LD YF+ S AK+P+FINLREVCGRHKLP G YVIVPSTF +EA FL+RIFSE+P +S ++DE+T ++ + +K + P +DA + L+ +F I+G D EIDA ELQ IL+ +FK++ FKF+GFS ET +SMVAMMD DQSG+LGF+EFKVLW DLRLWK FKKFD+D+SG FNSYE+R LN +GFR+SN++ +++ R+ANK+G++ FDDYIL VRL+T+F T+K+Q SK A+F QD F+QC++Y+
Sbjct: 94 SVGPSQD-YNNVKNSLRGK--LYEDPDFPAVNESLYFSQRPPRKIDWKRPHEICQNPEFISEGASRFDVRQGELGDCWLLAAVACLSLHSELFNQVVPSDQNFSKPSYCGMFRFRFWRFGAWKEVLVDDRLPTYNNKLVYMHSTDSNEFWSALLEKAYAKISGSYESLKGGSTSEALEDFTGGVTEFFDMKKEVPKNLFDIMFKAFSRCSLMGCSIEADPRKTEAQLPNGLIMGHAYSITDVKMVNINIPGKSGKIQLIRIRNPWGNEAEWKGSWSDKSPEWNYISQADRNTMGLTFDDDGEFWMSYQDFIKNFEKMEICHLGPQSLEADQLGGKKKSRWEMCIENGEWKKRLNAGGCRNFLDTFWTNPQFRVKVVDPDEDDDENLGTIIIGLMQKDMRKRRKEGVEFYTIGYAIYQLKDE--NQGPLDINYFKYNGSVAKAPSFINLREVCGRHKLPPGTYVIVPSTFEPNQEASFLMRIFSEKPNESGELDEQTAMIPTEPEKVVSP----ADDAQIMALRQSFNQIAGQDLEIDAYELQDILNTAFKKD-FKFDGFSIETSKSMVAMMDYDQSGKLGFDEFKVLWTDLRLWKEAFKKFDQDKSGNFNSYELRLCLNSVGFRLSNKILNSLVMRFANKEGLVLFDDYILCCVRLKTIFETYKSQPHDSKDRAMFDQDTFIQCIMYV 771
BLAST of Calpain-B vs. Planmine SMEST
Match: SMESG000039299.1 (SMESG000039299.1) HSP 1 Score: 834.713 bits (2155), Expect = 0.000e+0 Identity = 420/699 (60.09%), Postives = 535/699 (76.54%), Query Frame = 1
Query: 379 SLKESKDWFYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDR-DYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEADPTKVEAKAEKGLIKGHAYSITDVKSVN----SKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPES-ELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILDVSFKR-----------ENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYIXXXXXXXXXXXXXKAQQKTSKGEAIFPQDEFMQCVLYM 2424
S+ S+D + N K L+ K L+EDP FPA N SLY+ + PP+KI+WKRP E+ QN FI++G SRFDV QGELGDCWLLAAV+ LS++ L +QV+PSDQ+F + Y G F FR W+FG W +V+VDDRLPT +LVY+HS D NEFWSALLEKAYAK++GSYE+LKGGS+SEA+EDFTGG+TE FD+ P NL+DIM K +R SLMGCSIEADP K EA+ GLI GHAYSITDVK VN K G++ L+R+RNPWGN EW G++SDKSPEWN IS +R +GLTF+DDGEFWM++QDF+K F K+EICHL P+S E + G KKS+WEMCIENGEW+K ++AGGCRNF++TF NPQF+VKVVDPDE+D+EN GT+IIGLMQK+MR RRKEG + +TIGYAIYQ+KD ++ LD YF+ S AK+P+FINLREVCGRHKLP G YVIVPSTF +EA FL+RIFSE+P +S ++DE+T ++ + +K + P +DA + L+ +F I+G D EIDA ELQ IL+ +FK+ NFKF+GFS ET +SMVAMMD DQSG+LGF+EFKVLW DLRLWK FKKFD+D+SG FNSYE+R LN +GFR+SN++ +++ R+ANK+G++ FDDYIL VRL+T+F T+K+Q SK A+F QD F+QC++Y+
Sbjct: 94 SVGPSQD-YNNVKNSLRGK--LYEDPDFPAVNESLYFSQRPPRKIDWKRPHEICQNPEFISEGASRFDVRQGELGDCWLLAAVACLSLHSELFNQVVPSDQNFSKPSYCGMFRFRFWRFGAWKEVLVDDRLPTYNNKLVYMHSTDSNEFWSALLEKAYAKISGSYESLKGGSTSEALEDFTGGVTEFFDMKKEVPKNLFDIMFKAFSRCSLMGCSIEADPRKTEAQLPNGLIMGHAYSITDVKMVNINIPGKSGKIQLIRIRNPWGNEAEWKGSWSDKSPEWNYISQADRNTMGLTFDDDGEFWMSYQDFIKNFEKMEICHLGPQSLEADQLGGKKKSRWEMCIENGEWKKRLNAGGCRNFLDTFWTNPQFRVKVVDPDEDDDENLGTIIIGLMQKDMRKRRKEGVEFYTIGYAIYQLKDE--NQGPLDINYFKYNGSVAKAPSFINLREVCGRHKLPPGTYVIVPSTFEPNQEASFLMRIFSEKPNESGELDEQTAMIPTEPEKVVSP----ADDAQIMALRQSFNQIAGQDLEIDAYELQDILNTAFKKGVTRAGMEVNPANFKFDGFSIETSKSMVAMMDYDQSGKLGFDEFKVLWTDLRLWKEAFKKFDQDKSGNFNSYELRLCLNSVGFRLSNKILNSLVMRFANKEGLVLFDDYILCCVRLKTIFETYKSQPHDSKDRAMFDQDTFIQCIMYV 783
BLAST of Calpain-B vs. Planmine SMEST
Match: SMESG000039299.1 (SMESG000039299.1) HSP 1 Score: 829.321 bits (2141), Expect = 0.000e+0 Identity = 421/707 (59.55%), Postives = 535/707 (75.67%), Query Frame = 1
Query: 379 SLKESKDWFYNQKRMLKAKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDR-DYVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPNLWDIMMKGVTRASLMGCSIEADPTKVEAKAEKGLIKGHAYSITDVKSVN----SKRGRVNLVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSPES-ELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIP------PE--VKREEDANNVKLQNAFKVISGADGEIDADELQQILDVSFKR-----------ENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYIXXXXXXXXXXXXXKAQQKTSKGEAIFPQDEFMQCVLYM 2424
S+ S+D + N K L+ K L+EDP FPA N SLY+ + PP+KI+WKRP E+ QN FI++G SRFDV QGELGDCWLLAAV+ LS++ L +QV+PSDQ+F + Y G F FR W+FG W +V+VDDRLPT +LVY+HS D NEFWSALLEKAYAK++GSYE+LKGGS+SEA+EDFTGG+TE FD+ P NL+DIM K +R SLMGCSIEADP K EA+ GLI GHAYSITDVK VN K G++ L+R+RNPWGN EW G++SDKSPEWN IS +R +GLTF+DDGEFWM++QDF+K F K+EICHL P+S E + G KKS+WEMCIENGEW+K ++AGGCRNF++TF NPQF+VKVVDPDE+D+EN GT+IIGLMQK+MR RRKEG + +TIGYAIYQ+KD ++ LD YF+ S AK+P+FINLREVCGRHKLP G YVIVPSTF +EA FL+RIFSE+P +S ++DE+T ++ + + PE V +DA + L+ +F I+G D EIDA ELQ IL+ +FK+ NFKF+GFS ET +SMVAMMD DQSG+LGF+EFKVLW DLRLWK FKKFD+D+SG FNSYE+R LN +GFR+SN++ +++ R+ANK+G++ FDDYIL VRL+T+F T+K+Q SK A+F QD F+QC++Y+
Sbjct: 94 SVGPSQD-YNNVKNSLRGK--LYEDPDFPAVNESLYFSQRPPRKIDWKRPHEICQNPEFISEGASRFDVRQGELGDCWLLAAVACLSLHSELFNQVVPSDQNFSKPSYCGMFRFRFWRFGAWKEVLVDDRLPTYNNKLVYMHSTDSNEFWSALLEKAYAKISGSYESLKGGSTSEALEDFTGGVTEFFDMKKEVPKNLFDIMFKAFSRCSLMGCSIEADPRKTEAQLPNGLIMGHAYSITDVKMVNINIPGKSGKIQLIRIRNPWGNEAEWKGSWSDKSPEWNYISQADRNTMGLTFDDDGEFWMSYQDFIKNFEKMEICHLGPQSLEADQLGGKKKSRWEMCIENGEWKKRLNAGGCRNFLDTFWTNPQFRVKVVDPDEDDDENLGTIIIGLMQKDMRKRRKEGVEFYTIGYAIYQLKDE--NQGPLDINYFKYNGSVAKAPSFINLREVCGRHKLPPGTYVIVPSTFEPNQEASFLMRIFSEKPNESGELDEQTAMIPTESNPVVSNTATPVPEKVVSPADDAQIMALRQSFNQIAGQDLEIDAYELQDILNTAFKKGVTRAGMEVNPANFKFDGFSIETSKSMVAMMDYDQSGKLGFDEFKVLWTDLRLWKEAFKKFDQDKSGNFNSYELRLCLNSVGFRLSNKILNSLVMRFANKEGLVLFDDYILCCVRLKTIFETYKSQPHDSKDRAMFDQDTFIQCIMYV 795
BLAST of Calpain-B vs. Planmine SMEST
Match: SMESG000017238.1 (SMESG000017238.1) HSP 1 Score: 710.294 bits (1832), Expect = 0.000e+0 Identity = 373/685 (54.45%), Postives = 468/685 (68.32%), Query Frame = 1
Query: 415 KRMLKAKNSLFEDPFFPANNTSLYYREAPPQKIEWKRPKEMQQNVAFIADGLSRFDVVQGELGDCWLLAAVSSLSMYPALMHQVIPSDQDFDRD-YVGAFMFRLWQFGTWYDVVVDDRLPTVRGRLVYLHSKDLNEFWSALLEKAYAKVAGSYEALKGGSSSEAMEDFTGGITEMFDLGSSAPPN------LWDIMMKGVTRASLMGCSIEADPTKVEAKAEKGLIKGHAYSITDVKSVNSKRGRVN-----LVRVRNPWGNSCEWTGAYSDKSPEWNEISDVERKRLGLTFEDDGEFWMNFQDFVKYFTKLEICHLSP----ESELENKAGIKKSKWEMCIENGEWQKNVSAGGCRNFIETFHLNPQFKVKVVDPDENDNENTGTLIIGLMQKEMRSRRKEGQDLFTIGYAIYQMKDNAYDKSLLDKRYFQITASFAKSPTFINLREVCGRHKLPIGKYVIVPSTFNQGEEAKFLLRIFSEQPYDSKQIDEKTEIVAPDWKKEIPPEVKREEDANNVKLQNAFKVISGADGEIDADELQQILDVSFKRENFKFNGFSRETCRSMVAMMDTDQSGRLGFEEFKVLWDDLRLWKTVFKKFDEDQSGTFNSYEIRQALNHIGFRVSNRVFTAIITRYANKQGIITFDDYIXXXXXXXXXXXXXKAQQKTSKGEAIFPQDEFMQCVLY 2421
K L AK +L+ED FPAN S+Y+ +WKRP E+ + F G SRFDV QGELGDCWLLAAV+SLSM L QV P+DQ F + Y GAF F W +G W ++++DDRLPT RLV++HS+D NEFWSALLEKAYAK+ GSYE LKGGSSSEAMEDFTGGITE+ DL N L+ IM++ ++ SLMGCSI+A+P EAK GLI GHAYSIT VK +++K N LVR+RNPWG S EW G + D S W+ ISD ER LGL F++DGEFWM+F DFV F KLEICHL P ES L+N K WE ++ G W+K SAGGC NF ETF NPQF++ V DPD++D +N GT+IIGL+QK MRS + G DLF IGY +Y++ + D LDK +F+ A SP FIN+REVCGRH+LP G Y I+PSTF +EA FLLRIFSE+ K+IDE +++ D K + P E + +L++AFK ++G D EIDA EL+ +LD +F+RE F F F+ +TCRSMVAMMD D SGRLGFEEF+V+W DL+LWKTVFKK D+D SG+ +SYE+R LN IGFRVSN++FT ++ R+A K G I FDD++ VRL+TVF TFKAQ+K + AI QD F+Q +Y
Sbjct: 96 KEKLVAKGTLYEDEDFPANGESIYFTSNKGGNFQWKRPHEICSDPQFFVGGASRFDVCQGELGDCWLLAAVASLSMQTMLFSQVAPNDQSFAKGKYCGAFHFNFWHYGEWKEIIIDDRLPTRNNRLVFMHSRDSNEFWSALLEKAYAKLCGSYELLKGGSSSEAMEDFTGGITEIIDLKGDNRKNNESKEKLFQIMIQSHSKMSLMGCSIDANPKVTEAKLGNGLIMGHAYSITAVKQISAKVPGYNGDIIKLVRIRNPWGQS-EWNGEWGDSSDSWHHISDKERGVLGLNFDNDGEFWMSFNDFVNNFEKLEICHLGPQSVGESLLKNS---NKRTWESSVQQGAWKKGASAGGCLNFQETFWSNPQFRIVVTDPDKDDTDNMGTVIIGLLQKNMRSIKNRGNDLFPIGYVLYKLP--STDCGPLDKVFFRTNRKVAMSPAFINMREVCGRHRLPPGSYAIIPSTFEVNQEADFLLRIFSERANCGKEIDETSKM---DDKNLVLPVPVNESEIE--QLRSAFKAVAGDDMEIDASELKDVLDSAFRREAF-FKEFTIDTCRSMVAMMDIDLSGRLGFEEFQVMWLDLKLWKTVFKKEDKDGSGSLSSYELRTLLNAIGFRVSNKIFTNLVVRFATKDGTIEFDDFVHCCVRLKTVFQTFKAQKKDAHNNAIIDQDTFIQSSIY 768 The following BLAST results are available for this feature:
BLAST of Calpain-B vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Calpain-B vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Calpain-B vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Calpain-B vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Calpain-B vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Calpain-B vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Calpain-B vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Calpain-B vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Calpain-B vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Calpain-B vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Calpain-B vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Calpain-B vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Calpain-B vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Calpain-B vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30000946 ID=SMED30000946|Name=Calpain-B|organism=Schmidtea mediterranea sexual|type=transcript|length=2590bpback to top protein sequence of SMED30000946-orf-1 >SMED30000946-orf-1 ID=SMED30000946-orf-1|Name=SMED30000946-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=730bp MPRFNDSSNTNESVGSRYSINRFTPLSPDPKQTDSESIYGSRFHVSKSLKback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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