SMED30000943
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30000943 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 4
Homology
BLAST of SMED30000943 vs. TrEMBL
Match: A0A5J4NYQ5 (Uncharacterized protein OS=Paragonimus westermani OX=34504 GN=DEA37_0013730 PE=4 SV=1) HSP 1 Score: 100.138 bits (248), Expect = 1.904e-18 Identity = 71/243 (29.22%), Postives = 107/243 (44.03%), Query Frame = 1 Query: 148 VLLPYNSDLCNSSINGINKYQEFTINQQLNFETSSNTSDVLRSINDNINTTDMSSYLPWQQIEHENSQYYFCRVLVKPMGLKSQLHTEQGEYSFWDIRARINRLQPPVKCSDRLLLQFRHNQNLISTLDSHSARTVNITCKDSLPIIIPSSIRKLYITYF----STDPDSEGFM-INITTFIYFQGDFPISFQNETCRLQDGFWCGNKTNACIYRDMICDGLDSCFDGGSDENNCLYSSNNTV 861 ++LP NS+ C+ ++ F++++Q++F T S S W N Y C+V V P G Q QG S+W R R+ + C D L + F + L + N+TC ++ P I S R + + + S D S + + ++ ++ F+G F I+ + C GFWCGN+T C+Y M CD D CFDGGSDE C N TV Sbjct: 25 IILPENSEFCSE----YPVFRRFSLSRQMSFTTGSYNSS-------------------W------NHLLYVCKVRVHPNGRLLQYSYPQGHTSYWFHRLRVEIHF--ISCDDFLTVGFLPEEITQPALHT---EMRNLTCHETYPQIFDSKHRIVSLAWHTSSGSIDSASASQLDVQMSAYMRFEGTFGIADKILRCEAGSGFWCGNQTAVCVYGQMRCDQADGCFDGGSDEVGCPPPRNYTV 233
BLAST of SMED30000943 vs. TrEMBL
Match: A0A4V3S7F8 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_011354 PE=4 SV=1) HSP 1 Score: 99.7525 bits (247), Expect = 4.000e-18 Identity = 67/243 (27.57%), Postives = 104/243 (42.80%), Query Frame = 1 Query: 151 LLPYNSDLCNSSINGINKYQEFTINQQLNFETSSNTSDVLRSINDNINTTDMSSYLPWQQIEHENSQYYFCRVLVKPMGLKSQLHTEQGEYSFWDIRARIN-RLQPPVKCSDRLLLQFRHNQNLISTLDSHSARTVNITCKDSLPIIIPSSIRKLYITYF----STDPDSEGFMINITTFIYFQGDFPISFQNETCRLQDGFWCGNKTNACIYRDMICDGLDSCFDGGSDENNCLYSSNNTVP 864 +LP NS C ++ F++++Q++F T + W N +Y C+V + P G QG S+W R R++ R C D L + F + L + +TC ++ P I S R + +++ STD + + ++ ++ F+G+F + + + C GFWCGN T CIY M CD D CFDGGSDE C N T P Sbjct: 28 ILPENSIYCKD----YQSFRRFSLSRQMSFTTGIYNAS-------------------W------NHLFYVCKVRIHPNGRLLHYSYPQGHTSYWYHRLRVDIRF---ASCGDVLTVGFIPEETTQQLLQPEE---LQLTCHEAFPKIFDSKHRIISLSWHTETGSTDLITSELDVQMSAYMRFEGNFGLVDKIQRCEAGSGFWCGNDTAVCIYGQMRCDQADGCFDGGSDEVGCPPPRNYTAP 235
BLAST of SMED30000943 vs. TrEMBL
Match: A0A4S2JMJ1 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_011354 PE=4 SV=1) HSP 1 Score: 99.7525 bits (247), Expect = 4.247e-18 Identity = 67/243 (27.57%), Postives = 104/243 (42.80%), Query Frame = 1 Query: 151 LLPYNSDLCNSSINGINKYQEFTINQQLNFETSSNTSDVLRSINDNINTTDMSSYLPWQQIEHENSQYYFCRVLVKPMGLKSQLHTEQGEYSFWDIRARIN-RLQPPVKCSDRLLLQFRHNQNLISTLDSHSARTVNITCKDSLPIIIPSSIRKLYITYF----STDPDSEGFMINITTFIYFQGDFPISFQNETCRLQDGFWCGNKTNACIYRDMICDGLDSCFDGGSDENNCLYSSNNTVP 864 +LP NS C ++ F++++Q++F T + W N +Y C+V + P G QG S+W R R++ R C D L + F + L + +TC ++ P I S R + +++ STD + + ++ ++ F+G+F + + + C GFWCGN T CIY M CD D CFDGGSDE C N T P Sbjct: 28 ILPENSIYCKD----YQSFRRFSLSRQMSFTTGIYNAS-------------------W------NHLFYVCKVRIHPNGRLLHYSYPQGHTSYWYHRLRVDIRF---ASCGDVLTVGFIPEETTQQLLQPEE---LQLTCHEAFPKIFDSKHRIISLSWHTETGSTDLITSELDVQMSAYMRFEGNFGLVDKIQRCEAGSGFWCGNDTAVCIYGQMRCDQADGCFDGGSDEVGCPPPRNYTAP 235
BLAST of SMED30000943 vs. TrEMBL
Match: G7YEA9 (Uncharacterized protein OS=Clonorchis sinensis OX=79923 GN=CLF_105851 PE=4 SV=1) HSP 1 Score: 99.3673 bits (246), Expect = 5.101e-18 Identity = 57/179 (31.84%), Postives = 84/179 (46.93%), Query Frame = 1 Query: 343 NSQYYFCRVLVKPMGLKSQLHTEQGEYSFWDIRARIN-RLQPPVKCSDRLLLQFRHNQNLISTLDSHSARTVNITCKDSLPIIIPSSIRKLYITY----FSTDPDSEGFMINITTFIYFQGDFPISFQNETCRLQDGFWCGNKTNACIYRDMICDGLDSCFDGGSDENNCLYSSNNTVP 864 N +Y C+V + P G QG S+W R R++ R C D L + F + L + +TC ++ P I S R + +++ STD + + ++ ++ F+G+F + + + C GFWCGN T CIY M CD D CFDGGSDE C N T P Sbjct: 13 NHLFYVCKVRIHPNGRLLHYSYPQGHTSYWFHRLRVDIRF---ASCGDVLTIGFIPEETTQQLLQPEE---LQLTCHEAFPKIFDSKHRIISLSWRTETGSTDLITSELDVQMSAYMRFEGNFGLVDKIQRCEAGSGFWCGNDTAVCIYGQMRCDQADGCFDGGSDEVGCPPPRNYTAP 185
BLAST of SMED30000943 vs. TrEMBL
Match: A0A419PUQ0 (Uncharacterized protein (Fragment) OS=Clonorchis sinensis OX=79923 GN=CSKR_2368s PE=4 SV=1) HSP 1 Score: 98.2117 bits (243), Expect = 6.002e-18 Identity = 57/179 (31.84%), Postives = 84/179 (46.93%), Query Frame = 1 Query: 343 NSQYYFCRVLVKPMGLKSQLHTEQGEYSFWDIRARIN-RLQPPVKCSDRLLLQFRHNQNLISTLDSHSARTVNITCKDSLPIIIPSSIRKLYITY----FSTDPDSEGFMINITTFIYFQGDFPISFQNETCRLQDGFWCGNKTNACIYRDMICDGLDSCFDGGSDENNCLYSSNNTVP 864 N +Y C+V + P G QG S+W R R++ R C D L + F + L + +TC ++ P I S R + +++ STD + + ++ ++ F+G+F + + + C GFWCGN T CIY M CD D CFDGGSDE C N T P Sbjct: 13 NHLFYVCKVRIHPNGRLLHYSYPQGHTSYWFHRLRVDIRF---ASCGDVLTIGFIPEETTQQLLQPEE---LQLTCHEAFPKIFDSKHRIISLSWRTESGSTDLITSELDVQMSAYMRFEGNFGLVDKIQRCEAGSGFWCGNDTAVCIYGQMRCDQADGCFDGGSDEVGCPPPRNYTAP 185
BLAST of SMED30000943 vs. Planmine SMEST
Match: SMESG000049530.1 (SMESG000049530.1) HSP 1 Score: 950.658 bits (2456), Expect = 0.000e+0 Identity = 510/515 (99.03%), Postives = 511/515 (99.22%), Query Frame = 1 Query: 76 MINRXXXXXXXXXXXXXQCLAKRLVLLPYNSDLCNSSINGINKYQEFTINQQLNFETSSNTSDVLRSINDNINTTDMSSYLPWQQIEHENSQYYFCRVLVKPMGLKSQLHTEQGEYSFWDIRARINRLQPPVKCSDRLLLQFRHNQNLISTLDSHSARTVNITCKDSLPIIIPSSIRKLYITYFSTDPDSEGFMINITTFIYFQGDFPISFQNETCRLQDGFWCGNKTNACIYRDMICDGLDSCFDGGSDENNCLYSSNNTVPFRNVTHLTRTKGAFIDYXXXXXXXXXXXXXXXXXVCICNQHRSEVFFDDEEDNLDVEKSQEENADHMWSSLDALDQLEKPENDKEDNGNYCEEDGRLMHLTGKEPLIGLQTINCPATIKAEITASCTGNSLNKXXXXXXXXXXXXAKGDRGSTNLPASNSLTDLEIKEKRPDLQQMDFLTNTTSNVMTISQITDDDYVLEDVGSLLRKVDKSKIPEELRISSMNSSPQPEGEKPNSSVQETAIDYRSDYILV 1620 MINRKILILIFKFEIILQCL+KRLVLLPYNSDLCNSSINGINKYQEFTINQQLNFETSSNTSDVLRSINDNINTTDMSSYLPWQQIEHENSQYYFCRVLVKPMGLKSQLHTEQGEYSFWDIRARIN LQPPVKCS RLLLQFRHNQNLISTLDSHSARTVNITCKDSLPIIIPSSIRKLYITYFSTDPDSEGFMINITTFIYFQGDFPISFQNETCRLQDGFWCGNKTNACIYRDMICDGLDSCFDGGSDENNCLYSSNNTVPFRNVTHLTR KGAFIDYLPWLALAILPLILLMALVCICNQHRSEVFFDDEEDNLDVEKSQEENADHMWSSLDALDQLEKPENDKEDNGNYCEEDGRLMHLTGKEPLIGLQTINCPATIKAEITASCTGNSLNKDYEDIDEEPIIIAKGDRGSTNLPASNSLTDLEIKEKRPDL QMDFLTNTTSNVMTISQITDDDYVLEDVGSLLRKVDKSKIPEELRISSMNSSPQPEGEKPNSSVQETAIDYRSDYILV Sbjct: 1 MINRKILILIFKFEIILQCLSKRLVLLPYNSDLCNSSINGINKYQEFTINQQLNFETSSNTSDVLRSINDNINTTDMSSYLPWQQIEHENSQYYFCRVLVKPMGLKSQLHTEQGEYSFWDIRARINHLQPPVKCSGRLLLQFRHNQNLISTLDSHSARTVNITCKDSLPIIIPSSIRKLYITYFSTDPDSEGFMINITTFIYFQGDFPISFQNETCRLQDGFWCGNKTNACIYRDMICDGLDSCFDGGSDENNCLYSSNNTVPFRNVTHLTRIKGAFIDYLPWLALAILPLILLMALVCICNQHRSEVFFDDEEDNLDVEKSQEENADHMWSSLDALDQLEKPENDKEDNGNYCEEDGRLMHLTGKEPLIGLQTINCPATIKAEITASCTGNSLNKDYEDIDEEPIIIAKGDRGSTNLPASNSLTDLEIKEKRPDLPQMDFLTNTTSNVMTISQITDDDYVLEDVGSLLRKVDKSKIPEELRISSMNSSPQPEGEKPNSSVQETAIDYRSDYILV 515
BLAST of SMED30000943 vs. Planmine SMEST
Match: SMESG000002838.1 (SMESG000002838.1) HSP 1 Score: 107.457 bits (267), Expect = 2.375e-24 Identity = 80/222 (36.04%), Postives = 123/222 (55.41%), Query Frame = 1 Query: 343 NSQYYFCRVLVKPMGLKSQLHTEQGEYSFWDIRARINRLQPPVKCSDRLLLQF-RHNQNLISTLDSHSARTV--NITCKDSLPIIIPSSIRKLYITYFSTD-PDSEGFMINI--TTFIYFQGDFPISFQNETCRLQDGFWCGNKTNACIYRDMICDGLDSCFDGGSDENNCLYSSNNTVPFRNVTHLTRTKGAFIDYXXXXXXXXXXXXXXXXXVCICNQHR 990 NS + C V+VKP G G SFW +R + + + CS+ L++ F + ++ S D +++ N +C D PI++ ++ RKL I+++S+D D E MI+I +T++ F G+F + + C + +G+WC N CIY M CD D CFDGGSDE C N T N + + + ++ YLPW+ L +LPLIL+ +LV ICNQ R Sbjct: 66 NSLRFVCSVIVKPFGKPITPPDVDGVLSFWKLRFKA---RTKLSCSNMLMMHFLKEDKTYDSPNDIIEEKSMMYNYSCNDKEPIMLNTNHRKLLISWYSSDNTDLEQQMIDIQLSTYMSFHGNFTKAQKESKCLMYNGYWCDNHVQVCIYAQMRCDQADGCFDGGSDEKGCPIPPNVTY---NGLNSRQRRDSWSGYLPWIVLGVLPLILIFSLVLICNQRR 281 The following BLAST results are available for this feature:
BLAST of SMED30000943 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30000943 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30000943 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30000943 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30000943 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30000943 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30000943 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30000943 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30000943 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30000943 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30000943 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30000943 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30000943 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30000943 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 2
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30000943 ID=SMED30000943|Name=SMED30000943|organism=Schmidtea mediterranea sexual|type=transcript|length=1925bpback to top protein sequence of SMED30000943-orf-1 >SMED30000943-orf-1 ID=SMED30000943-orf-1|Name=SMED30000943-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=516bp MINRKILILIFKFEIILQCLAKRLVLLPYNSDLCNSSINGINKYQEFTINback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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