SMED30000928

Overview
NameSMED30000928
Smed IDSMED30000928
Length (bp)1882
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30000928 (SMED30000928) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30000928 (SMED30000928) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30000928

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
testis primordiumSMED30000928SMESG000032160.1 PL08001A2H03_SE3GPL10652PMID:20844018
Wang et al., 2010
whole organism juvenile cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Alignments
SMED30000928 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.003604:35219..37063 +9216
Homology
BLAST of SMED30000928 vs. Planmine SMEST
Match: SMESG000032160.1 (SMESG000032160.1)

HSP 1 Score: 1130.93 bits (2924), Expect = 0.000e+0
Identity = 596/597 (99.83%), Postives = 596/597 (99.83%), Query Frame = 1
Query:   70 MSVSSIFKYINNLLSDAEDETEDDFSEMSFSAIEKNSKQKSTKISHFNNKIRKKYSICKQKKYFSKGLILSNKNLIIFAKAFSKIDKSPTKILHLPFWTEMFSWCNSQVLVKSLISYKCISIPNTILRPFPIGSGHSSLKFANKIKIKQEENFTTLNEKKFLLPKQFKSKIKIAVVEEILAKEVFNSYFIGEIQKIVLMNSGVQTIKTKKYHQDFVLEIFFNISEIMKEKLPSLFFIGDIEIDNVILSKSEILFSFLTARLTSSNSFSPKLISIHSFLKKNNPNEMTLKXXXXXXXXXXXXXVSLISPDQTEFQNSLKIENVNEKYSKKDSKNLSAFSLSNSSIKRXXXXXXXXXXXXXPNSREHHQLQSKKVTSISKNTIPYFDQKLLNSLTDISYQKYEKYTKKLNQIIRFPFLVAIQNRKKHFEFPNTDQISENSYRSSPYNEWLLAVFPRGLSRGINNSLKGISYKFSIYVLEDVNCHKLFLHFHDISSVNMTKQQFQQWCTEKCQFNTAEIYDDLSKIVSVKNRSNHKQNDQNILKIIETKFNCLVLLSDRLMDRNYGYCRAIQFVGVNKNSILESLKFFIKDVRGIELKTS 1860
            MSVSSIFKYINNLLSDAEDETEDDFSEMSFSAIEKNSKQKSTKISHFNNKIRKKYSICKQKKYFSKGLILSNKNLIIFAKAFSKIDKSPTKILHLPFWTEMFSWCNSQVLVKSLISYKCISIPNTILRPFPIGSGHSSLKFANKIKIKQEENFTTLNEKKFLLPKQFKSKIKIAVVEEILAKEVFNSYFIGEIQKIVLMNSGVQTIKTKKYHQDFVLEIFFNISEIMKEKLPSLFFIGDIEIDNVILSKSEILFSFLTARLTSSNSFSPKLISIHSFLKKNNPNEMTLKNPHHHNNIIPIINVSLISPDQTEFQNSLKIENVNEKYS KDSKNLSAFSLSNSSIKRKLSSELKTLEKKLPNSREHHQLQSKKVTSISKNTIPYFDQKLLNSLTDISYQKYEKYTKKLNQIIRFPFLVAIQNRKKHFEFPNTDQISENSYRSSPYNEWLLAVFPRGLSRGINNSLKGISYKFSIYVLEDVNCHKLFLHFHDISSVNMTKQQFQQWCTEKCQFNTAEIYDDLSKIVSVKNRSNHKQNDQNILKIIETKFNCLVLLSDRLMDRNYGYCRAIQFVGVNKNSILESLKFFIKDVRGIELKTS
Sbjct:    1 MSVSSIFKYINNLLSDAEDETEDDFSEMSFSAIEKNSKQKSTKISHFNNKIRKKYSICKQKKYFSKGLILSNKNLIIFAKAFSKIDKSPTKILHLPFWTEMFSWCNSQVLVKSLISYKCISIPNTILRPFPIGSGHSSLKFANKIKIKQEENFTTLNEKKFLLPKQFKSKIKIAVVEEILAKEVFNSYFIGEIQKIVLMNSGVQTIKTKKYHQDFVLEIFFNISEIMKEKLPSLFFIGDIEIDNVILSKSEILFSFLTARLTSSNSFSPKLISIHSFLKKNNPNEMTLKNPHHHNNIIPIINVSLISPDQTEFQNSLKIENVNEKYSNKDSKNLSAFSLSNSSIKRKLSSELKTLEKKLPNSREHHQLQSKKVTSISKNTIPYFDQKLLNSLTDISYQKYEKYTKKLNQIIRFPFLVAIQNRKKHFEFPNTDQISENSYRSSPYNEWLLAVFPRGLSRGINNSLKGISYKFSIYVLEDVNCHKLFLHFHDISSVNMTKQQFQQWCTEKCQFNTAEIYDDLSKIVSVKNRSNHKQNDQNILKIIETKFNCLVLLSDRLMDRNYGYCRAIQFVGVNKNSILESLKFFIKDVRGIELKTS 597          
The following BLAST results are available for this feature:
BLAST of SMED30000928 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000928 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000928 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000928 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000928 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000928 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000928 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000928 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000928 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000928 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000928 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000928 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000928 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000928 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000032160.10.000e+099.83SMESG000032160.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30000928 ID=SMED30000928|Name=SMED30000928|organism=Schmidtea mediterranea sexual|type=transcript|length=1882bp
TCGTCCTTGAAATATCCCCCGGGATTGATTAATTAATTAAAATAAAGTTT
GATTAATTATAGAAACCTAATGTCAGTCAGTAGCATTTTCAAATATATAA
ATAATCTCCTGTCTGATGCTGAGGATGAAACTGAGGATGATTTCAGTGAA
ATGTCCTTTAGTGCAATTGAGAAAAATTCAAAACAAAAATCTACTAAAAT
CAGCCATTTTAATAACAAAATCCGTAAAAAATACTCAATTTGCAAACAGA
AAAAATATTTCAGCAAAGGACTGATTTTGTCCAACAAAAATTTGATTATT
TTCGCAAAAGCATTTTCGAAAATCGACAAATCTCCGACAAAAATCCTTCA
TTTGCCATTTTGGACGGAAATGTTTAGTTGGTGTAATTCTCAAGTCCTGG
TGAAAAGTCTCATTTCATACAAATGCATATCTATCCCGAATACAATTCTG
CGCCCATTTCCTATTGGCTCCGGCCACAGTTCACTTAAATTCGCCAATAA
GATCAAAATAAAACAAGAAGAGAACTTTACAACTTTAAATGAAAAGAAAT
TCCTTCTGCCGAAACAATTTAAATCGAAAATAAAGATCGCAGTTGTTGAA
GAAATTTTAGCCAAAGAAGTTTTCAATTCGTATTTCATTGGAGAAATTCA
AAAAATCGTATTAATGAATTCAGGCGTTCAAACGATTAAAACAAAGAAAT
ATCATCAAGATTTTGTTTTAGAAATATTTTTTAATATCAGTGAAATTATG
AAAGAAAAATTGCCGAGTCTTTTCTTTATAGGCGATATTGAAATTGACAA
TGTGATACTTAGCAAAAGTGAAATCCTATTTTCCTTTCTAACAGCCAGAC
TTACATCGAGCAATTCATTTTCCCCCAAATTAATTTCTATTCATTCCTTC
TTGAAAAAAAATAATCCAAATGAAATGACACTGAAAAATCCCCATCATCA
TAATAATATTATTCCGATAATAAATGTATCCTTAATCAGTCCTGACCAAA
CCGAATTTCAAAATTCTCTAAAAATTGAAAATGTAAACGAAAAATATTCC
AAAAAAGATTCCAAAAATTTGTCCGCATTTTCATTGTCTAATTCAAGCAT
AAAAAGAAAACTTTCATCGGAATTGAAAACCCTGGAGAAAAAATTACCAA
ACTCACGGGAGCATCACCAATTGCAGTCAAAAAAGGTGACTAGCATCAGC
AAAAATACCATTCCTTATTTTGATCAAAAGCTTTTGAATTCTCTTACCGA
TATCTCGTATCAAAAGTATGAAAAATATACGAAAAAATTAAACCAAATTA
TTCGATTTCCATTTTTAGTGGCAATTCAAAATAGAAAAAAACATTTCGAA
TTCCCCAACACCGATCAAATTTCGGAGAATTCCTACCGATCCTCTCCTTA
CAATGAATGGTTGTTAGCCGTTTTTCCTAGGGGCCTCAGCCGAGGCATTA
ATAATAGTTTAAAAGGAATTAGTTATAAATTTTCGATCTATGTTTTGGAA
GACGTGAATTGCCACAAACTTTTTCTCCATTTTCATGACATTTCTTCGGT
AAACATGACTAAGCAACAGTTTCAACAGTGGTGCACTGAGAAATGCCAAT
TTAATACTGCTGAAATTTATGATGATTTATCTAAAATAGTCTCCGTAAAA
AACCGTTCAAATCACAAGCAAAATGATCAAAACATTCTAAAAATCATAGA
AACCAAGTTCAATTGCCTGGTATTGCTATCTGATAGATTGATGGACAGAA
ATTACGGATATTGCCGAGCTATTCAATTTGTAGGAGTTAACAAGAACTCA
ATTTTAGAATCTTTAAAATTTTTTATAAAAGATGTCAGAGGGATTGAATT
AAAAACATCTTAAAATTTAAAAAAAATTAAAA
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protein sequence of SMED30000928-orf-1

>SMED30000928-orf-1 ID=SMED30000928-orf-1|Name=SMED30000928-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=598bp
MSVSSIFKYINNLLSDAEDETEDDFSEMSFSAIEKNSKQKSTKISHFNNK
IRKKYSICKQKKYFSKGLILSNKNLIIFAKAFSKIDKSPTKILHLPFWTE
MFSWCNSQVLVKSLISYKCISIPNTILRPFPIGSGHSSLKFANKIKIKQE
ENFTTLNEKKFLLPKQFKSKIKIAVVEEILAKEVFNSYFIGEIQKIVLMN
SGVQTIKTKKYHQDFVLEIFFNISEIMKEKLPSLFFIGDIEIDNVILSKS
EILFSFLTARLTSSNSFSPKLISIHSFLKKNNPNEMTLKNPHHHNNIIPI
INVSLISPDQTEFQNSLKIENVNEKYSKKDSKNLSAFSLSNSSIKRKLSS
ELKTLEKKLPNSREHHQLQSKKVTSISKNTIPYFDQKLLNSLTDISYQKY
EKYTKKLNQIIRFPFLVAIQNRKKHFEFPNTDQISENSYRSSPYNEWLLA
VFPRGLSRGINNSLKGISYKFSIYVLEDVNCHKLFLHFHDISSVNMTKQQ
FQQWCTEKCQFNTAEIYDDLSKIVSVKNRSNHKQNDQNILKIIETKFNCL
VLLSDRLMDRNYGYCRAIQFVGVNKNSILESLKFFIKDVRGIELKTS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0003304testis primordium
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 345..365