Glutathione S-transferase

Overview
NameGlutathione S-transferase
Smed IDSMED30000883
Length (bp)1042
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Glutathione S-transferase (SMED30000883) t-SNE clustered cells

Violin plots show distribution of expression levels for Glutathione S-transferase (SMED30000883) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Glutathione S-transferase (SMED30000883) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Glutathione S-transferase (SMED30000883) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30000883

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
protonephridiaSMED30000883h1SMcG0019812 dd_Smed_v4_1517_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30000883h1SMcG0019812 dd_Smed_v4_1517_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30000883h1SMcG0019812 dd_Smed_v4_1517_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30000883h1SMcG0019812 dd_Smed_v4_1517_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30000883h1SMcG0019812 dd_Smed_v4_1517_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30000883h1SMcG0019812 dd_Smed_v4_1517_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Glutathione S-transferase vs. Ensembl Human
Match: GSTM4 (glutathione S-transferase mu 4 [Source:HGNC Symbol;Acc:HGNC:4636])

HSP 1 Score: 245.358 bits (625), Expect = 2.676e-80
Identity = 111/216 (51.39%), Postives = 160/216 (74.07%), Query Frame = 3
Query:  135 MAPMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFG 782
            M+  LGYW IRGLA  IRLLLEYT   Y+EK Y +G+APD++R +WLNEKF LGL FPNLPYLI+G  K+TQS+AIL YIA KH++ G + EE+  V+++ ++  D+     R+CYSPDF KLK +Y+ +LP+ ++ FS FLG + W +G+K+++ DF  YD+LD  ++  PNCLD F N+K +I++FE L+ I+ Y+KS++++ KPL  +VA +G
Sbjct:    1 MSMTLGYWDIRGLAHAIRLLLEYTDSSYEEKKYTMGDAPDYDRSQWLNEKFKLGLDFPNLPYLIDGAHKITQSNAILCYIARKHNLCGETEEEKIRVDILENQAMDVSNQLARVCYSPDFEKLKPEYLEELPTMMQHFSQFLGKRPWFVGDKITFVDFLAYDVLDLHRIFEPNCLDAFPNLKDFISRFEGLEKISAYMKSSRFLPKPLYTRVAVWG 216          
BLAST of Glutathione S-transferase vs. Ensembl Human
Match: GSTM4 (glutathione S-transferase mu 4 [Source:HGNC Symbol;Acc:HGNC:4636])

HSP 1 Score: 243.047 bits (619), Expect = 2.738e-79
Identity = 109/216 (50.46%), Postives = 160/216 (74.07%), Query Frame = 3
Query:  135 MAPMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFG 782
            M+  LGYW IRGLA  IRLLLEYT   Y+EK Y +G+APD++R +WLNEKF LGL FPNLPYLI+G  K+TQS+AIL YIA KH++ G + EE+  V+++ ++  D+     R+CYSPDF KLK +Y+ +LP+ ++ FS FLG + W +G+K+++ DF  YD+LD  ++  PNCLD F N+K +I++FE L+ I+ Y+KS++++ +P+  K+A +G
Sbjct:    1 MSMTLGYWDIRGLAHAIRLLLEYTDSSYEEKKYTMGDAPDYDRSQWLNEKFKLGLDFPNLPYLIDGAHKITQSNAILCYIARKHNLCGETEEEKIRVDILENQAMDVSNQLARVCYSPDFEKLKPEYLEELPTMMQHFSQFLGKRPWFVGDKITFVDFLAYDVLDLHRIFEPNCLDAFPNLKDFISRFEGLEKISAYMKSSRFLPRPVFTKMAVWG 216          
BLAST of Glutathione S-transferase vs. Ensembl Human
Match: GSTM3 (glutathione S-transferase mu 3 [Source:HGNC Symbol;Acc:HGNC:4635])

HSP 1 Score: 240.736 bits (613), Expect = 2.463e-78
Identity = 111/214 (51.87%), Postives = 153/214 (71.50%), Query Frame = 3
Query:  144 MLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFGG 785
            +LGYW IRGLA  IRLLLE+T   Y+EK Y  G APD++R +WL+ KF L L FPNLPYL++G  K+TQS+AILRYIA KH+M G + EE+  V+++ +++ D R   +RLCYS D  KLK  Y+ +LP +LK FS FLG   W  GEKL++ DF  YDILD  ++  P CLD+F N+K ++ +FE L+ IA YL+S+++   P+NNK+A++G 
Sbjct:    8 VLGYWDIRGLAHAIRLLLEFTDTSYEEKRYTCGEAPDYDRSQWLDVKFKLDLDFPNLPYLLDGKNKITQSNAILRYIARKHNMCGETEEEKIRVDIIENQVMDFRTQLIRLCYSSDHEKLKPQYLEELPGQLKQFSMFLGKFSWFAGEKLTFVDFLTYDILDQNRIFDPKCLDEFPNLKAFMCRFEALEKIAAYLQSDQFCKMPINNKMAQWGN 221          
BLAST of Glutathione S-transferase vs. Ensembl Human
Match: GSTM3 (glutathione S-transferase mu 3 [Source:HGNC Symbol;Acc:HGNC:4635])

HSP 1 Score: 240.736 bits (613), Expect = 2.463e-78
Identity = 111/214 (51.87%), Postives = 153/214 (71.50%), Query Frame = 3
Query:  144 MLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFGG 785
            +LGYW IRGLA  IRLLLE+T   Y+EK Y  G APD++R +WL+ KF L L FPNLPYL++G  K+TQS+AILRYIA KH+M G + EE+  V+++ +++ D R   +RLCYS D  KLK  Y+ +LP +LK FS FLG   W  GEKL++ DF  YDILD  ++  P CLD+F N+K ++ +FE L+ IA YL+S+++   P+NNK+A++G 
Sbjct:    8 VLGYWDIRGLAHAIRLLLEFTDTSYEEKRYTCGEAPDYDRSQWLDVKFKLDLDFPNLPYLLDGKNKITQSNAILRYIARKHNMCGETEEEKIRVDIIENQVMDFRTQLIRLCYSSDHEKLKPQYLEELPGQLKQFSMFLGKFSWFAGEKLTFVDFLTYDILDQNRIFDPKCLDEFPNLKAFMCRFEALEKIAAYLQSDQFCKMPINNKMAQWGN 221          
BLAST of Glutathione S-transferase vs. Ensembl Human
Match: GSTM2 (glutathione S-transferase mu 2 [Source:HGNC Symbol;Acc:HGNC:4634])

HSP 1 Score: 240.35 bits (612), Expect = 2.830e-78
Identity = 110/216 (50.93%), Postives = 158/216 (73.15%), Query Frame = 3
Query:  135 MAPMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFG 782
            M   LGYW IRGLA  IRLLLEYT   Y+EK Y +G+APD++R +WLNEKF LGL FPNLPYLI+G  K+TQS+AILRYIA KH++ G S +E+   +++ ++  D RM   +LCY PDF KLK +Y+  LP  LKL+S FLG + W +G+K+++ DF  YD+L+  ++  P+CLD F N+K +I++FE L+ I+ Y+KS++++ +P+  K+A +G
Sbjct:    1 MPMTLGYWNIRGLAHSIRLLLEYTDSSYEEKKYTMGDAPDYDRSQWLNEKFKLGLDFPNLPYLIDGTHKITQSNAILRYIARKHNLCGESEKEQIREDILENQFMDSRMQLAKLCYDPDFEKLKPEYLQALPEMLKLYSQFLGKQPWFLGDKITFVDFIAYDVLERNQVFEPSCLDAFPNLKDFISRFEGLEKISAYMKSSRFLPRPVFTKMAVWG 216          
BLAST of Glutathione S-transferase vs. Ensembl Celegans
Match: gst-1 (Glutathione S-transferase P [Source:UniProtKB/Swiss-Prot;Acc:P10299])

HSP 1 Score: 90.8929 bits (224), Expect = 1.988e-21
Identity = 64/215 (29.77%), Postives = 107/215 (49.77%), Query Frame = 3
Query:  135 MAPMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQ-LPSRL----KLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNN 764
            M   L Y+ I GLA+PIRLLL      Y++             ++W + K  +   F  +P L+ GD ++ QS AI+R++A  + + GS+  E   ++M  + ++DL   +  + Y  ++   K  YI   LP  L    KLF  +   + ++IG+K SY D+  ++ LD   +L+PN LD    +K +  +F +   I  YL     I+ P+N 
Sbjct:    1 MTLKLTYFDIHGLAEPIRLLLADKQVAYEDH--------RVTYEQWADIKPKM--IFGQVPCLLSGDEEIVQSGAIIRHLARLNGLNGSNETETTFIDMFYEGLRDLHTKYTTMIYR-NYEDGKAPYIKDVLPGELARLEKLFHTYKNGEHYVIGDKESYADYVLFEELDIHLILTPNALDGVPALKKFHERFAERPNIKAYLNKRAAINPPVNG 204          
BLAST of Glutathione S-transferase vs. Ensembl Celegans
Match: gst-1 (Glutathione S-transferase P [Source:UniProtKB/Swiss-Prot;Acc:P10299])

HSP 1 Score: 90.8929 bits (224), Expect = 1.988e-21
Identity = 64/215 (29.77%), Postives = 107/215 (49.77%), Query Frame = 3
Query:  135 MAPMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQ-LPSRL----KLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNN 764
            M   L Y+ I GLA+PIRLLL      Y++             ++W + K  +   F  +P L+ GD ++ QS AI+R++A  + + GS+  E   ++M  + ++DL   +  + Y  ++   K  YI   LP  L    KLF  +   + ++IG+K SY D+  ++ LD   +L+PN LD    +K +  +F +   I  YL     I+ P+N 
Sbjct:    1 MTLKLTYFDIHGLAEPIRLLLADKQVAYEDH--------RVTYEQWADIKPKM--IFGQVPCLLSGDEEIVQSGAIIRHLARLNGLNGSNETETTFIDMFYEGLRDLHTKYTTMIYR-NYEDGKAPYIKDVLPGELARLEKLFHTYKNGEHYVIGDKESYADYVLFEELDIHLILTPNALDGVPALKKFHERFAERPNIKAYLNKRAAINPPVNG 204          
BLAST of Glutathione S-transferase vs. Ensembl Celegans
Match: gst-23 (Glutathione S-Transferase [Source:UniProtKB/TrEMBL;Acc:P91505])

HSP 1 Score: 90.1225 bits (222), Expect = 4.750e-21
Identity = 65/203 (32.02%), Postives = 109/203 (53.69%), Query Frame = 3
Query:  135 MAPMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQ-LPSRL----KLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYL 728
            M   L Y+ IRGLA+PIRLLL       QE  +E       +R+EW   K ++   F  LP L  G   + QS+AILR++   + + GS+  E + ++M ++ ++DLR  +  +  + D+   K+ YI++ LP  L    KLF  F   + ++ G+KLSY D+  +++LD   +L+P  L+    +K +  +F +   +  YL
Sbjct:    1 MTLKLTYFDIRGLAEPIRLLLND-----QEIPFEDNR---ISREEWAEMKSTM--IFAQLPCLHIGTETIVQSAAILRHLGRIYGLNGSTESETSFIDMFSEGLRDLRTKYFTMIKT-DYEDGKEAYITETLPGELLRIEKLFCTFNNGENFVSGDKLSYADYALFELLDIHLILTPTALNGTPALKRFHGRFAERPNMRLYL 192          
BLAST of Glutathione S-transferase vs. Ensembl Celegans
Match: gst-25 (Glutathione S-Transferase [Source:UniProtKB/TrEMBL;Acc:O61750])

HSP 1 Score: 84.3445 bits (207), Expect = 5.188e-19
Identity = 58/215 (26.98%), Postives = 108/215 (50.23%), Query Frame = 3
Query:  135 MAPMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQ-LPSRL----KLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNN 764
            M     Y+  RG+ +PIR+LL  T +E     +       F+ ++W + K ++   F + P L  GD ++ QS  I+R++A  H + G++ EE   ++M  + ++DL  ++  + Y  ++   +  +I   LP  L    KLF  +   + ++ G  +SY D+  ++ LD  K+LSP  LD    +K +  +F +   + +YLK    +  P+N 
Sbjct:    1 MTLKFSYFGTRGIGEPIRMLL--TDQEVAFIDHR------FSWEQWADIKPTM--IFGSCPCLTMGDKEIVQSGTIIRHLARIHGLNGTNEEETIFIDMFYEGVRDLHATYATMIYQ-NYENGRQPFIQDVLPEHLARLEKLFRTYENGENFVAGANISYVDYVLFEELDIQKILSPTALDRTPQLKKFHEQFGERKNLTEYLKKRAIMKTPVNG 204          
BLAST of Glutathione S-transferase vs. Ensembl Celegans
Match: gst-10 (Glutathione S-transferase P 10 [Source:UniProtKB/Swiss-Prot;Acc:Q9N4X8])

HSP 1 Score: 83.9593 bits (206), Expect = 7.596e-19
Identity = 56/204 (27.45%), Postives = 105/204 (51.47%), Query Frame = 3
Query:  138 APMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQ-LPSRLKLFSDFL--GSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNK 740
             P L Y+ IRG  + IRLL    G ++++  +      D+  +EW  ++F  G+    LP L     ++ Q+ AI+R++   H + GS+ +E   ++M  + ++D+RM +VR  Y  +    ++D +++ +P  L    +     S  ++IG K+SY D+  ++ LD   +L  N LD F  +K++  +  K   +  YL+  K
Sbjct:    3 VPQLYYFTIRGFGEYIRLLFLDNGIKFEDIRF------DYEGNEW--QEFKKGMLLGQLPCLKVDGQEIVQTGAIMRHLGRVHGLNGSNEQEATFLDMFFEGVRDVRMKYVRYIYYDE--GTREDCVNKTIPEALVKLEELFKAHSGDFIIGNKISYADYILFEELDVYHVLDANILDKFPTLKSFWERMWKRPNLNAYLEKRK 196          
BLAST of Glutathione S-transferase vs. Ensembl Fly
Match: GstS1 (gene:FBgn0010226 transcript:FBtr0087005)

HSP 1 Score: 56.9954 bits (136), Expect = 4.658e-9
Identity = 46/202 (22.77%), Postives = 96/202 (47.52%), Query Frame = 3
Query:  147 LGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRL------KLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIK-MLSPNCLDDFENIKTYINKFEKLDPIAKYLK 731
            L Y+ ++ LA+P+R L  Y  +EY++            RDEW   K ++ +    +P L     ++ QS ++ R++A+   + G++  E   +++V D I D R+    + Y P+  ++K+  +  L + +      KL      +   L   KL++ D  F  I D +  M+  + L+ +  ++  ++    L+PI  +++
Sbjct:   52 LFYFNVKALAEPLRYLFAYGNQEYEDV--------RVTRDEWPALKPTMPMG--QMPVLEVDGKRVHQSISMARFLAKTVGLCGATPWEDLQIDIVVDTINDFRLKIAVVSYEPED-EIKEKKLVTLNAEVIPFYLEKLEQTVKDNDGHLALGKLTWADVYFAGITDYMNYMVKRDLLEPYPALRGVVDAVNALEPIKAWIE 242          
BLAST of Glutathione S-transferase vs. Ensembl Fly
Match: GstS1 (gene:FBgn0010226 transcript:FBtr0100258)

HSP 1 Score: 56.9954 bits (136), Expect = 4.658e-9
Identity = 46/202 (22.77%), Postives = 96/202 (47.52%), Query Frame = 3
Query:  147 LGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRL------KLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIK-MLSPNCLDDFENIKTYINKFEKLDPIAKYLK 731
            L Y+ ++ LA+P+R L  Y  +EY++            RDEW   K ++ +    +P L     ++ QS ++ R++A+   + G++  E   +++V D I D R+    + Y P+  ++K+  +  L + +      KL      +   L   KL++ D  F  I D +  M+  + L+ +  ++  ++    L+PI  +++
Sbjct:   52 LFYFNVKALAEPLRYLFAYGNQEYEDV--------RVTRDEWPALKPTMPMG--QMPVLEVDGKRVHQSISMARFLAKTVGLCGATPWEDLQIDIVVDTINDFRLKIAVVSYEPED-EIKEKKLVTLNAEVIPFYLEKLEQTVKDNDGHLALGKLTWADVYFAGITDYMNYMVKRDLLEPYPALRGVVDAVNALEPIKAWIE 242          
BLAST of Glutathione S-transferase vs. Ensembl Fly
Match: GstS1 (gene:FBgn0010226 transcript:FBtr0087006)

HSP 1 Score: 56.9954 bits (136), Expect = 4.658e-9
Identity = 46/202 (22.77%), Postives = 96/202 (47.52%), Query Frame = 3
Query:  147 LGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRL------KLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIK-MLSPNCLDDFENIKTYINKFEKLDPIAKYLK 731
            L Y+ ++ LA+P+R L  Y  +EY++            RDEW   K ++ +    +P L     ++ QS ++ R++A+   + G++  E   +++V D I D R+    + Y P+  ++K+  +  L + +      KL      +   L   KL++ D  F  I D +  M+  + L+ +  ++  ++    L+PI  +++
Sbjct:   52 LFYFNVKALAEPLRYLFAYGNQEYEDV--------RVTRDEWPALKPTMPMG--QMPVLEVDGKRVHQSISMARFLAKTVGLCGATPWEDLQIDIVVDTINDFRLKIAVVSYEPED-EIKEKKLVTLNAEVIPFYLEKLEQTVKDNDGHLALGKLTWADVYFAGITDYMNYMVKRDLLEPYPALRGVVDAVNALEPIKAWIE 242          
BLAST of Glutathione S-transferase vs. Ensembl Fly
Match: GstS1 (gene:FBgn0010226 transcript:FBtr0333551)

HSP 1 Score: 53.1434 bits (126), Expect = 7.812e-8
Identity = 47/203 (23.15%), Postives = 98/203 (48.28%), Query Frame = 3
Query:  147 LGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVR-LCYSPDFIKLKDDYISQLPSRL------KLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIK-MLSPNCLDDFENIKTYINKFEKLDPIAKYLK 731
            L Y+ ++ LA+P+R L  Y  +EY++            RDEW   K ++ +    +P L     ++ QS ++ R++A+   + G++  E   +++V D I D R+S  + + Y P+  ++K+  +  L + +      KL      +   L   KL++ D  F  I D +  M+  + L+ +  ++  ++    L+PI  +++
Sbjct:   52 LFYFNVKALAEPLRYLFAYGNQEYEDV--------RVTRDEWPALKPTMPMG--QMPVLEVDGKRVHQSISMARFLAKTVGLCGATPWEDLQIDIVVDTINDFRLSSEQFVSYEPE-DEIKEKKLVTLNAEVIPFYLEKLEQTVKDNDGHLALGKLTWADVYFAGITDYMNYMVKRDLLEPYPALRGVVDAVNALEPIKAWIE 243          
BLAST of Glutathione S-transferase vs. Ensembl Zebrafish
Match: gstm.3 (glutathione S-transferase mu tandem duplicate 3 [Source:ZFIN;Acc:ZDB-GENE-050309-24])

HSP 1 Score: 243.817 bits (621), Expect = 8.598e-80
Identity = 112/216 (51.85%), Postives = 158/216 (73.15%), Query Frame = 3
Query:  135 MAPMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFG 782
            MA  L YW IRG+AQP+RLLLE+TG +Y+EK Y  G APD+++  W NEK  LGL+FPNLPYL +GD K+ QS+AILRYIA K+++ G + EE+  V+++ ++  D R  F++LCY  DF K K  Y  +LP  LK FSDFLG ++W  G+K+++ DF  YD+LD  +ML P CLDD+ N++++++ FE L+ I  Y+KSNKY+  P+NNK+AK+G
Sbjct:    1 MAMKLAYWDIRGIAQPVRLLLEFTGTKYEEKFYSCGEAPDYDKSCWFNEKNKLGLAFPNLPYLEDGDTKVVQSNAILRYIARKNNLCGETEEEQTRVDILENQAMDFRNGFIQLCYG-DFDKNKPCYCEKLPGSLKQFSDFLGDRKWFAGDKITFVDFIMYDLLDLHRMLHPECLDDYRNLRSFLDHFESLEKIVAYMKSNKYMKTPVNNKMAKWG 215          
BLAST of Glutathione S-transferase vs. Ensembl Zebrafish
Match: gstm.1 (glutathione S-transferase mu, tandem duplicate 1 [Source:ZFIN;Acc:ZDB-GENE-030911-2])

HSP 1 Score: 242.662 bits (618), Expect = 2.494e-79
Identity = 111/216 (51.39%), Postives = 157/216 (72.69%), Query Frame = 3
Query:  135 MAPMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFG 782
            MA  L YW IRGLAQPIRLLLEYTG +Y+EK Y  G AP++++  W NEK  LG+ FPNLPYL +GD K+ QS+AI+RYIA KH++ G + EE+  V+++ ++  D R  FV+LCY  DF K K  Y  +L   LK FSDFLG ++W  G+K+++ DF  Y++LD  +M  P CLDDF+N++ +++ FE L+ IA+Y+KSN+++  P+NNK+AK+G
Sbjct:    1 MAMKLAYWDIRGLAQPIRLLLEYTGTKYEEKFYTCGEAPNYDKSCWFNEKSKLGMDFPNLPYLEDGDRKIVQSNAIMRYIARKHNLCGETEEEQMRVDILENQAMDFRNGFVQLCY-LDFDKNKSSYCEKLSGTLKQFSDFLGDRKWFAGDKITFVDFIMYELLDQHRMFEPACLDDFKNLRCFLDHFESLEKIAEYMKSNRFMKTPVNNKMAKWG 215          
BLAST of Glutathione S-transferase vs. Ensembl Zebrafish
Match: gstm.2 (glutathione S-transferase mu tandem duplicate 2 [Source:ZFIN;Acc:ZDB-GENE-080218-30])

HSP 1 Score: 241.506 bits (615), Expect = 6.558e-79
Identity = 112/216 (51.85%), Postives = 159/216 (73.61%), Query Frame = 3
Query:  135 MAPMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFG 782
            MA  L YW IRGLAQPIRLLLEYTG +Y+EKLY  G AP+++R  WLN+K  L + FPNLPYL +GD K+ QS+AI+RYIA KH++ G + EE+  V+++ ++  D R  FV+LCY  DF K K  Y  +LP  LK FSDFLG ++W  G+K+++ DF  Y++LD  +M  P CLDDF+N++ +++ FE L+ IA+Y+KSN+++  P+N+K+AK+G
Sbjct:    1 MAMKLVYWDIRGLAQPIRLLLEYTGTKYEEKLYSCGEAPNYDRSCWLNDKSKLKMDFPNLPYLEDGDRKIVQSNAIMRYIARKHNLCGETEEEQIRVDILENQAMDFRNGFVQLCYF-DFDKNKSSYCEKLPGTLKQFSDFLGDRKWFAGDKITFVDFIMYELLDQHRMFEPACLDDFKNLRCFLDHFENLEKIAEYMKSNRFMKTPVNSKMAKWG 215          
BLAST of Glutathione S-transferase vs. Ensembl Zebrafish
Match: gstm.3 (glutathione S-transferase mu tandem duplicate 3 [Source:ZFIN;Acc:ZDB-GENE-050309-24])

HSP 1 Score: 144.436 bits (363), Expect = 1.996e-42
Identity = 65/121 (53.72%), Postives = 90/121 (74.38%), Query Frame = 3
Query:  135 MAPMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFI 497
            MA  L YW IRG+AQP+RLLLE+TG +Y+EK Y  G APD+++  W NEK  LGL+FPNLPYL +GD K+ QS+AILRYIA K+++ G + EE+  V+++ ++  D R  F++LCY  DF+
Sbjct:    1 MAMKLAYWDIRGIAQPVRLLLEFTGTKYEEKFYSCGEAPDYDKSCWFNEKNKLGLAFPNLPYLEDGDTKVVQSNAILRYIARKNNLCGETEEEQTRVDILENQAMDFRNGFIQLCYG-DFV 120          
BLAST of Glutathione S-transferase vs. Ensembl Zebrafish
Match: gstp1 (glutathione S-transferase pi 1 [Source:ZFIN;Acc:ZDB-GENE-020806-4])

HSP 1 Score: 116.316 bits (290), Expect = 1.557e-30
Identity = 60/213 (28.17%), Postives = 119/213 (55.87%), Query Frame = 3
Query:  135 MAP-MLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQ--WLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNN 764
            MAP  L Y+ ++G    ++++L    ++ +E L           +EW+         F  LP   +GD  L QS+A+LR++  KH   G +  E ++++++ D ++DLR+ +++L Y  ++   K+ +I  LP+ LK F + L   +  +L+G+++S+ D+N +D+L  +K+LSP+CLD F ++K++++K      +   L+   +   P+N 
Sbjct:    1 MAPYTLTYFAVKGRCGALKIMLADKDQQLKENLVTF--------EEWMKGDLKATCVFGQLPKFEDGDLVLFQSNAMLRHLGRKHAAYGKNDSEASLIDVMNDGVEDLRLKYIKLIYQ-EYETGKEAFIKDLPNHLKCFENVLAKNKTGFLVGDQISFADYNLFDLLLNLKVLSPSCLDSFPSLKSFVDKISARPKVKALLECENFKKLPING 204          
BLAST of Glutathione S-transferase vs. Ensembl Xenopus
Match: krt8.2 (keratin 8 [Source:Xenbase;Acc:XB-GENE-876686])

HSP 1 Score: 219.935 bits (559), Expect = 2.414e-70
Identity = 101/213 (47.42%), Postives = 150/213 (70.42%), Query Frame = 3
Query:  144 MLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFG 782
            +LGYW IRGLA  IRLLLEYTG +Y+EK Y  G+AP++++ +WL+EK  LGL FPNLPYL++GD KL+QS+AILRYIA KH + G S +E+  V ++  +I   R + +    +P +  LK  Y+ +LP  LK FS FLG + W  G+K+++ DF  YD+LD  ++L P CL +F+N++ ++ +FE L  I  Y+KS++++  PLNN++A +G
Sbjct:    2 ILGYWDIRGLAHSIRLLLEYTGTQYEEKKYITGDAPNYDKTQWLDEKEKLGLDFPNLPYLLDGDVKLSQSNAILRYIARKHGLCGESEKEKMYVLLLHTQIDAFRSTIIIFAQAPRYETLKGPYLEKLPIALKRFSCFLGDRSWFAGDKITFVDFVVYDVLDQHQILEPTCLQNFKNLQDFLKRFEALPAIDAYMKSSRFMKMPLNNRMASWG 214          
BLAST of Glutathione S-transferase vs. Ensembl Xenopus
Match: nemp1 (nuclear envelope integral membrane protein 1 [Source:Xenbase;Acc:XB-GENE-974573])

HSP 1 Score: 117.087 bits (292), Expect = 1.228e-30
Identity = 71/211 (33.65%), Postives = 111/211 (52.61%), Query Frame = 3
Query:  153 YWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSL--GLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLG----SKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNK 767
            Y+ +RG A+ IRLLL   G +++E   ++         +W++ K        F  LP   +GDF L QS++ILRY+  KH + GS   ERA+V+MV   ++DLR  + R  Y  ++   KD Y+  LP++L LF   L        +++G K+SY D+N  D L     LSP CL ++  +  Y+ +      +AKYL S+    +P+  K
Sbjct:    8 YFPVRGRAEAIRLLLADQGIDWEEDEVQIA--------DWVSGKDGRKKDAVFGQLPGFRDGDFVLYQSNSILRYLGNKHGLDGSDAHERAVVDMVNSGVEDLRQKYGRFLYF-EYETGKDKYLETLPAQLDLFERILSKNANGSAFIVGNKISYADYNLLDTLHCHIDLSPTCLSNYPLLSAYVERLVSRPKLAKYLNSDVCKRRPITPK 209          
BLAST of Glutathione S-transferase vs. Ensembl Xenopus
Match: gnrhr (gonadotropin releasing hormone receptor [Source:Xenbase;Acc:XB-GENE-5753573])

HSP 1 Score: 114.39 bits (285), Expect = 1.657e-29
Identity = 76/225 (33.78%), Postives = 114/225 (50.67%), Query Frame = 3
Query:  114 ILLYLFNMAP--MLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFL-----GSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNK 767
            +L Y F +     L Y+ +RG A+ IRLLL      ++E   +  +    N DE     F        LP    GDF L QS++ILRY+A KH + G + EE A ++MV D ++DLR  + R  Y     K K  Y+  LP +L+ F   L     GSK +++G+K+SY D+N  D+L     LSP CL  F  ++ Y+ +      ++ YL S+    +P+  K
Sbjct:    9 LLYYFFPLVSGYTLTYYPVRGRAEAIRLLLADQDIPWEEDEVQWQDWCSGNHDERKKAVFG------RLPQFQNGDFVLCQSNSILRYLAHKHGLTGDNDEESAHIDMVNDSVEDLRKKYGRFIYFTCQEKGKGKYLEGLPQQLEFFERVLSKNHNGSK-FVVGQKISYADYNLVDLLQCHLDLSPECLSAFPLLRAYLERLVSQPTLSDYLNSDARKRRPITLK 226          
BLAST of Glutathione S-transferase vs. Ensembl Xenopus
Match: gnrhr (gonadotropin releasing hormone receptor [Source:Xenbase;Acc:XB-GENE-5753573])

HSP 1 Score: 113.235 bits (282), Expect = 3.950e-29
Identity = 73/213 (34.27%), Postives = 109/213 (51.17%), Query Frame = 3
Query:  144 MLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFL-----GSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNK 767
             L Y+ +RG A+ IRLLL      ++E   +  +    N DE     F        LP    GDF L QS++ILRY+A KH + G + EE A ++MV D ++DLR  + R  Y     K K  Y+  LP +L+ F   L     GSK +++G+K+SY D+N  D+L     LSP CL  F  ++ Y+ +      ++ YL S+    +P+  K
Sbjct:    5 TLTYFPVRGRAEAIRLLLADQDIPWEEDEVQWQDWCSGNHDERKKAVFG------RLPQFQNGDFVLCQSNSILRYLAHKHGLTGDNDEESAHIDMVNDSVEDLRKKYGRFIYFTCQEKGKGKYLEGLPQQLEFFERVLSKNHNGSK-FVVGQKISYADYNLVDLLQCHLDLSPECLSAFPLLRAYLERLVSQPTLSDYLNSDARKRRPITLK 210          
BLAST of Glutathione S-transferase vs. Ensembl Xenopus
Match: gnrhr (gonadotropin releasing hormone receptor [Source:Xenbase;Acc:XB-GENE-5753573])

HSP 1 Score: 104.375 bits (259), Expect = 5.850e-26
Identity = 72/213 (33.80%), Postives = 115/213 (53.99%), Query Frame = 3
Query:  144 MLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFL-----GSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNK 767
             L Y+ +RG A+ IRLLL   G  +++   E    P +   +   +K ++   F  LP    GD+ L QS++ILRY+  KH + GS+ EE   ++MV D ++DLR  +VRL ++ ++   KD Y+  LP +L+ F   L     GSK +++G+K+SY D+N  D+L     LSP  L  F  +  Y+ +      + +YLKS     +P+  K
Sbjct:    5 TLTYFPVRGRAEAIRLLLADQGVAFKD---EEAQIPLWFAGKDDRKKHAV---FGQLPQFKNGDYTLYQSNSILRYLGRKHGLNGSNDEELGHIDMVNDGVEDLRQKYVRLIFT-EYDTGKDKYLEALPQQLEFFERILSKNHNGSK-FIVGQKISYADYNLLDLLHCHLDLSPKSLSAFPLLSAYVERLNSRPKLNEYLKSEARNRRPITPK 209          
BLAST of Glutathione S-transferase vs. Ensembl Mouse
Match: Gstm4 (glutathione S-transferase, mu 4 [Source:MGI Symbol;Acc:MGI:95862])

HSP 1 Score: 249.98 bits (637), Expect = 3.017e-82
Identity = 114/216 (52.78%), Postives = 156/216 (72.22%), Query Frame = 3
Query:  135 MAPMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFG 782
            M   LGYW IRGLA  IRLLLEYTG  Y+EK Y +G+APD++R +WL+EKF LGL FPNLPYLI+G  K+TQS+AILRYIA KH++ G + EE+  V+++ ++  D+     R+CYSPDF KLK +Y+ QLP  +KLFS FLG + W +GEK+++ DF  YDILD   +  P CLD F N+K ++ +FE L  I+ Y+K+++++  PL  KVA +G
Sbjct:    1 MPMTLGYWDIRGLAHAIRLLLEYTGSSYEEKRYTMGDAPDYDRSQWLSEKFKLGLDFPNLPYLIDGSHKITQSNAILRYIARKHNLCGETEEEKIRVDILENQAMDVSNQLARVCYSPDFEKLKVEYLEQLPGMVKLFSQFLGQRTWFVGEKITFVDFLAYDILDLHLIFEPTCLDAFPNLKDFVARFEVLKRISAYMKTSRFLRTPLYTKVATWG 216          
BLAST of Glutathione S-transferase vs. Ensembl Mouse
Match: Gstm1 (glutathione S-transferase, mu 1 [Source:MGI Symbol;Acc:MGI:95860])

HSP 1 Score: 245.743 bits (626), Expect = 1.721e-80
Identity = 109/216 (50.46%), Postives = 155/216 (71.76%), Query Frame = 3
Query:  135 MAPMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFG 782
            M  +LGYW +RGL  PIR+LLEYT   Y EK Y +G+APDF+R +WLNEKF LGL FPNLPYLI+G  K+TQS+AILRY+A KH + G + EER   ++V +++ D RM  + LCY+PDF K K +++  +P ++KL+S+FLG + W  G+K++Y DF  YDILD  +M  P CLD F N++ ++ +FE L  I+ Y+KS++YI  P+ +K+A + 
Sbjct:    1 MPMILGYWNVRGLTHPIRMLLEYTDSSYDEKRYTMGDAPDFDRSQWLNEKFKLGLDFPNLPYLIDGSHKITQSNAILRYLARKHHLDGETEEERIRADIVENQVMDTRMQLIMLCYNPDFEKQKPEFLKTIPEKMKLYSEFLGKRPWFAGDKVTYVDFLAYDILDQYRMFEPKCLDAFPNLRDFLARFEGLKKISAYMKSSRYIATPIFSKMAHWS 216          
BLAST of Glutathione S-transferase vs. Ensembl Mouse
Match: Gstm5 (glutathione S-transferase, mu 5 [Source:MGI Symbol;Acc:MGI:1309466])

HSP 1 Score: 238.039 bits (606), Expect = 1.765e-77
Identity = 110/214 (51.40%), Postives = 154/214 (71.96%), Query Frame = 3
Query:  144 MLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFGG 785
            +LGYW IRGLA  IR+LLE+T   Y+EK Y  G APD++R +WL+ KF L L FPNLPYL++G  K+TQS+AILRYIA KH+M G + EE+  V+++ ++I D RM  VRLCY+ +   LK  Y+ QLP++LK FS FLG   W  GEKL++ DF  YD+LD  ++  P CLD+F N+K ++ +FE L+ IA +L+S+++   P+NNK+AK+G 
Sbjct:    7 VLGYWDIRGLAHAIRMLLEFTDTSYEEKRYICGEAPDYDRSQWLDVKFKLDLDFPNLPYLMDGKNKITQSNAILRYIARKHNMCGDTEEEKIRVDIMENQIMDFRMQLVRLCYNSNHENLKPQYLEQLPAQLKQFSLFLGKFTWFAGEKLTFVDFLTYDVLDQNRIFEPKCLDEFPNLKAFMCRFEALEKIAAFLQSDRFFKMPINNKMAKWGN 220          
BLAST of Glutathione S-transferase vs. Ensembl Mouse
Match: Gstm2 (glutathione S-transferase, mu 2 [Source:MGI Symbol;Acc:MGI:95861])

HSP 1 Score: 236.113 bits (601), Expect = 8.059e-77
Identity = 107/213 (50.23%), Postives = 153/213 (71.83%), Query Frame = 3
Query:  135 MAPMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVA 773
            M   LGYW IRGLA  IRLLLEYT   Y++K Y +G+APD++R +WL+EKF LGL FPNLPYLI+G  K+TQS+AILRY+A KH++ G + EER  V+++ ++  D R+    +CYSPDF K K +Y+  LP ++KL+S+FLG + W  G K++Y DF  YD+LD  ++  P CLD F N+K ++ +FE L  I+ Y+KS++++ KP+  K+A
Sbjct:    1 MPMTLGYWDIRGLAHAIRLLLEYTDTSYEDKKYTMGDAPDYDRSQWLSEKFKLGLDFPNLPYLIDGSHKITQSNAILRYLARKHNLCGETEEERIRVDILENQAMDTRIQLAMVCYSPDFEKKKPEYLEGLPEKMKLYSEFLGKQPWFAGNKVTYVDFLVYDVLDQHRIFEPKCLDAFPNLKDFMGRFEGLKKISDYMKSSRFLSKPIFAKMA 213          
BLAST of Glutathione S-transferase vs. Ensembl Mouse
Match: Gstm3 (glutathione S-transferase, mu 3 [Source:MGI Symbol;Acc:MGI:106026])

HSP 1 Score: 236.113 bits (601), Expect = 1.012e-76
Identity = 104/218 (47.71%), Postives = 156/218 (71.56%), Query Frame = 3
Query:  135 MAPMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFGGD 788
            M   LGYW  RGL   IRLLLEYT   Y+EK Y +G+AP+F+R +WL+EKF+LGL FPNLPYLI+G  K+TQS+AILRY+  KH++ G + EER  V+ + +++ D R+  + +C SPDF K K +++  +P ++KL+S+FLG + W  G+K++Y DF  YDILD  +M  P CLD F N++ ++ +FE L  I+ Y+KS++++ +P+  K+A++G D
Sbjct:    1 MPMTLGYWNTRGLTHSIRLLLEYTDSSYEEKRYVMGDAPNFDRSQWLSEKFNLGLDFPNLPYLIDGSHKVTQSNAILRYLGRKHNLCGETEEERIRVDTLENQVMDTRIQLMIVCCSPDFEKQKPEFLKAIPEKMKLYSEFLGKRPWFAGDKVTYVDFLAYDILDQYRMFEPKCLDAFPNLRDFLARFEGLKKISAYMKSSRFLPRPVFTKIAQWGTD 218          
BLAST of Glutathione S-transferase vs. UniProt/SwissProt
Match: sp|Q9N0V4|GSTM1_BOVIN (Glutathione S-transferase Mu 1 OS=Bos taurus OX=9913 GN=GSTM1 PE=1 SV=3)

HSP 1 Score: 248.44 bits (633), Expect = 8.486e-81
Identity = 113/216 (52.31%), Postives = 161/216 (74.54%), Query Frame = 3
Query:  135 MAPMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFG 782
            M  +LGYW IRGLA  IRLLLEYT   Y+E+ Y +G+APD++R +WLNEKF LGL FPNLPYLI+G  KLTQS+AILRYIA KH++ G + EE   V+++ +++ D+R++  R+CYSPDF KLK  ++ ++P ++KLFS+FLG + W  G+KL+Y DF  YD+LD  ++  P CLD F N+K +I++FE L  I+ Y+KS++++  PL  K+A +G
Sbjct:    1 MPMILGYWDIRGLAHAIRLLLEYTDTNYEERQYSVGDAPDYDRSQWLNEKFKLGLDFPNLPYLIDGTHKLTQSNAILRYIARKHNLCGETEEEMIRVDILENQVMDVRLAMARICYSPDFEKLKPGFLKEIPEKIKLFSEFLGKRPWFAGDKLTYVDFLVYDVLDMHRIFEPKCLDAFPNLKDFISRFEGLKKISAYMKSSRFLPGPLFMKLAVWG 216          
BLAST of Glutathione S-transferase vs. UniProt/SwissProt
Match: sp|P10649|GSTM1_MOUSE (Glutathione S-transferase Mu 1 OS=Mus musculus OX=10090 GN=Gstm1 PE=1 SV=2)

HSP 1 Score: 245.743 bits (626), Expect = 1.204e-79
Identity = 109/216 (50.46%), Postives = 155/216 (71.76%), Query Frame = 3
Query:  135 MAPMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFG 782
            M  +LGYW +RGL  PIR+LLEYT   Y EK Y +G+APDF+R +WLNEKF LGL FPNLPYLI+G  K+TQS+AILRY+A KH + G + EER   ++V +++ D RM  + LCY+PDF K K +++  +P ++KL+S+FLG + W  G+K++Y DF  YDILD  +M  P CLD F N++ ++ +FE L  I+ Y+KS++YI  P+ +K+A + 
Sbjct:    1 MPMILGYWNVRGLTHPIRMLLEYTDSSYDEKRYTMGDAPDFDRSQWLNEKFKLGLDFPNLPYLIDGSHKITQSNAILRYLARKHHLDGETEEERIRADIVENQVMDTRMQLIMLCYNPDFEKQKPEFLKTIPEKMKLYSEFLGKRPWFAGDKVTYVDFLAYDILDQYRMFEPKCLDAFPNLRDFLARFEGLKKISAYMKSSRYIATPIFSKMAHWS 216          
BLAST of Glutathione S-transferase vs. UniProt/SwissProt
Match: sp|Q03013|GSTM4_HUMAN (Glutathione S-transferase Mu 4 OS=Homo sapiens OX=9606 GN=GSTM4 PE=1 SV=3)

HSP 1 Score: 245.358 bits (625), Expect = 1.285e-79
Identity = 111/216 (51.39%), Postives = 160/216 (74.07%), Query Frame = 3
Query:  135 MAPMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFG 782
            M+  LGYW IRGLA  IRLLLEYT   Y+EK Y +G+APD++R +WLNEKF LGL FPNLPYLI+G  K+TQS+AIL YIA KH++ G + EE+  V+++ ++  D+     R+CYSPDF KLK +Y+ +LP+ ++ FS FLG + W +G+K+++ DF  YD+LD  ++  PNCLD F N+K +I++FE L+ I+ Y+KS++++ KPL  +VA +G
Sbjct:    1 MSMTLGYWDIRGLAHAIRLLLEYTDSSYEEKKYTMGDAPDYDRSQWLNEKFKLGLDFPNLPYLIDGAHKITQSNAILCYIARKHNLCGETEEEKIRVDILENQAMDVSNQLARVCYSPDFEKLKPEYLEELPTMMQHFSQFLGKRPWFVGDKITFVDFLAYDVLDLHRIFEPNCLDAFPNLKDFISRFEGLEKISAYMKSSRFLPKPLYTRVAVWG 216          
BLAST of Glutathione S-transferase vs. UniProt/SwissProt
Match: sp|P16413|GSTMU_CAVPO (Glutathione S-transferase B OS=Cavia porcellus OX=10141 GN=GSTM1 PE=1 SV=1)

HSP 1 Score: 244.202 bits (622), Expect = 4.109e-79
Identity = 113/212 (53.30%), Postives = 157/212 (74.06%), Query Frame = 3
Query:  141 PM-LGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVA 773
            PM LGYW IRGL  PIRL+LEYT   Y+EK Y +G+APD++R +WLNEKF LGL FPNLPYLI+G  KLTQS+AILRYIA KH++ G + EE   ++++ +++ D+RM  + LCYSPDF + K +++  +P ++KLFS FLG   W  G KL+Y DF  YD+LD  +ML P CL+ F N+K +I++FE L+ I+ Y+KS++++ KPL +K A
Sbjct:    1 PMTLGYWNIRGLTHPIRLILEYTNSGYEEKRYNMGDAPDYDRSQWLNEKFKLGLDFPNLPYLIDGTHKLTQSNAILRYIARKHNLCGVTEEETIRMDILENQVMDIRMQLIMLCYSPDFEQKKAEFLEGIPDKMKLFSQFLGKLPWFAGNKLTYVDFLAYDVLDQYRMLEPKCLEAFPNLKDFISRFEGLEKISSYMKSSRFLPKPLFSKFA 212          
BLAST of Glutathione S-transferase vs. UniProt/SwissProt
Match: sp|Q9BEB0|GSTM2_MACFU (Glutathione S-transferase Mu 2 OS=Macaca fuscata fuscata OX=9543 GN=GSTM2 PE=2 SV=3)

HSP 1 Score: 243.817 bits (621), Expect = 6.145e-79
Identity = 111/216 (51.39%), Postives = 160/216 (74.07%), Query Frame = 3
Query:  135 MAPMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFG 782
            M   LGYW IRGLA  IRLLLEYTG  Y+EK Y +G+APD++R +WLNEKF LGL FPNLPYLI+G  K+TQS+AILRYIA KH++ G + +E+   +++ +++ D RM   RLCY PDF KLK +Y+  LP  LKL+S FLG + W +G+K+++ DF  YD+L+  ++  P+CLD F N+K +I++FE L+ I+ Y+KS++++ +P+  K+A +G
Sbjct:    1 MPMTLGYWNIRGLAHSIRLLLEYTGSSYEEKKYTMGDAPDYDRSQWLNEKFKLGLDFPNLPYLIDGTHKITQSNAILRYIARKHNLCGETEKEKIREDILENQLMDNRMQLARLCYDPDFEKLKPEYLEGLPEMLKLYSQFLGKQPWFLGDKITFVDFIAYDVLERNQVFEPSCLDAFPNLKDFISRFEGLEKISAYMKSSRFLPRPVFTKMAVWG 216          
BLAST of Glutathione S-transferase vs. TrEMBL
Match: G9BRW2 (Glutathione S-transferase OS=Dugesia japonica OX=6161 GN=GST PE=2 SV=1)

HSP 1 Score: 355.91 bits (912), Expect = 1.585e-120
Identity = 167/219 (76.26%), Postives = 194/219 (88.58%), Query Frame = 3
Query:  135 MAPMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFGGDT 791
            MAP+LGYWKIRGLAQ IRLLLEYTGEEY EK YELGN  DFNRD+WLNEKFSLGLSFPNLPYLI+GD KLTQSSAILRY+AEKH+MVG + EERA   M+A+E+QDLRM F RLCY+PDF  LK +Y+SQLPSRLKLFSDF+G+K WL+GEKL+YPDF+FYD+LD +K+LSP CLD+F+N+K Y+  FEKL+PIAKY+KS+KYI KPLNNKV KFGGD 
Sbjct:    1 MAPLLGYWKIRGLAQSIRLLLEYTGEEYNEKYYELGN--DFNRDDWLNEKFSLGLSFPNLPYLIDGDLKLTQSSAILRYLAEKHNMVGETSEERARTMMLAEEVQDLRMGFARLCYNPDFANLKHEYLSQLPSRLKLFSDFIGTKHWLMGEKLTYPDFHFYDMLDSLKILSPTCLDEFDNLKNYLENFEKLEPIAKYMKSDKYIQKPLNNKVVKFGGDC 217          
BLAST of Glutathione S-transferase vs. TrEMBL
Match: A0A151N8K8 (Glutathione S-transferase Mu 3 OS=Alligator mississippiensis OX=8496 GN=GSTM3 PE=4 SV=1)

HSP 1 Score: 273.478 bits (698), Expect = 4.433e-88
Identity = 122/216 (56.48%), Postives = 166/216 (76.85%), Query Frame = 3
Query:  135 MAPMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFG 782
            MA +LGYW IRGLA  IRLLLEYTG  Y++KLY  G APD+++ +W+NEK  LGL FPNLPYLI+G  KLTQS+AILRYIA KHDM G + EE+  ++M+ +++ D RMS V +CY+PDF KLK  Y+ QLP +LKLFS FLG ++W  GEK+++ DF  YD+LD  +M  P CLD F+N+K ++++FE LD IA Y++S++++  P+NNK+AK+G
Sbjct:    1 MAAVLGYWDIRGLAHAIRLLLEYTGTTYEDKLYSCGEAPDYDKSQWINEKEKLGLDFPNLPYLIDGKTKLTQSNAILRYIARKHDMCGETEEEKVRLDMLENQVMDFRMSLVMVCYNPDFEKLKPGYLEQLPGKLKLFSQFLGDRKWFAGEKITFVDFLMYDVLDQNRMFEPKCLDQFKNLKDFLDRFEALDKIAAYMRSDRFMKTPINNKMAKWG 216          
BLAST of Glutathione S-transferase vs. TrEMBL
Match: F8TTE3 (Glutathione S-transferase mu 3 OS=Meleagris gallopavo OX=9103 PE=2 SV=1)

HSP 1 Score: 259.61 bits (662), Expect = 1.366e-82
Identity = 115/214 (53.74%), Postives = 162/214 (75.70%), Query Frame = 3
Query:  144 MLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFGG 785
            +LGYW IRGLA  IRLLLE+TG  Y++KLY  G APD+++ +W+NEK  LGL FPNLPY I+GD KLTQS+AILRYIA KH+M G + EER  V+++ +++ D RMS V +CYSPDF KLK  Y+ QLP +L+LFS FLG ++W  GEKL++ DF  +D+LD  ++ +P CL+ + N++ ++ +F  L+ +A YLKS+++   P+NNK+AK+GG
Sbjct:    3 VLGYWDIRGLAHAIRLLLEHTGTPYEDKLYSCGEAPDYDKSQWINEKEKLGLDFPNLPYFIDGDVKLTQSNAILRYIARKHNMCGDTEEERQRVDVLENQVMDFRMSLVMVCYSPDFEKLKPGYLEQLPGKLRLFSTFLGDRKWFAGEKLTFVDFLMFDVLDQNRIFAPRCLEPYSNLRAFMERFGALEKVAAYLKSSRFQKMPINNKMAKWGG 216          
BLAST of Glutathione S-transferase vs. TrEMBL
Match: I1SZ68 (Glutathione S-transferase 1 OS=Spirometra erinaceieuropaei OX=99802 PE=2 SV=1)

HSP 1 Score: 258.84 bits (660), Expect = 2.257e-82
Identity = 118/217 (54.38%), Postives = 160/217 (73.73%), Query Frame = 3
Query:  141 PMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFGGDT 791
            P+L YWKIRGLAQ IRLL  Y G+EY E+LYE G  PDF+R+ WL  KF+ GL FPNLPYLI+  FK+TQS AIL Y+A+KH M+ +S +ERA+++M+ +E+   R S+ RL Y+PDF KLK  ++ +LP +L+L+S++LG KQWL G+K+ YPDFN YD LD ++ L P+CL DF N++ Y+ +FE LD I ++ KS+ YI  P NN VA + GD 
Sbjct:    5 PVLEYWKIRGLAQAIRLLFHYLGQEYVEELYEAGGPPDFSREPWLKHKFNKGLDFPNLPYLIDDGFKITQSGAILEYVADKHGMLPTSAKERAVLHMLQNELSGFRASYGRLVYNPDFSKLKQGFLDELPQKLRLWSNYLGDKQWLTGDKIQYPDFNLYDFLDALQNLEPSCLKDFPNLQRYMKRFEDLDKIKEFQKSDAYIAHPFNNTVAAWRGDA 221          
BLAST of Glutathione S-transferase vs. TrEMBL
Match: A0A098LZU6 (Glutathione S-transferase Mu 1-like protein OS=Hypsiglena sp. JMG-2014 OX=1550645 PE=4 SV=1)

HSP 1 Score: 258.455 bits (659), Expect = 3.026e-82
Identity = 115/216 (53.24%), Postives = 162/216 (75.00%), Query Frame = 3
Query:  135 MAPMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFG 782
            M  +LGYW IRGLA  IRLLLEYTG  Y++KLY  G APD+++ +W+NEK  LGL FPNLPYLI+G  KLTQS+AILRYIA KH + G + EE   ++M+ +++ D RM+ V +CY+PDF KLK  Y+ QLP +LKLFS FLG ++W  G+K+++ DF  YD+LD  +M  P CLD F+N++ ++++FE L+ IA Y+ S++++  P+NNK+AK+G
Sbjct:    1 MTMILGYWDIRGLAHSIRLLLEYTGTPYEDKLYSCGEAPDYDKSQWINEKEKLGLDFPNLPYLIDGKTKLTQSNAILRYIARKHHLCGKTEEELIRIDMLENQVMDFRMALVMICYNPDFEKLKPGYLEQLPGKLKLFSQFLGDRKWFAGDKITFVDFLMYDVLDQNRMFEPKCLDQFKNLQDFLSRFEALEKIAAYMSSSRFMKTPVNNKMAKWG 216          
BLAST of Glutathione S-transferase vs. Ensembl Cavefish
Match: ENSAMXT00000030565.1 (glutathione S-transferase Mu 3 [Source:NCBI gene;Acc:103025109])

HSP 1 Score: 223.787 bits (569), Expect = 5.376e-72
Identity = 102/212 (48.11%), Postives = 153/212 (72.17%), Query Frame = 3
Query:  147 LGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFG 782
            L YW IRGLAQPIRLLLEYTG +Y++K Y  G APD+++  W NEK  L + FPNLPYL +GD K++QS+AI+RYI  KH++ G + +E+  V+++ ++  D R  FV LCY  ++ + K  Y+  LP  LK FSDFLG ++W  G+K+++ DF  Y++LD  +M  P CLD+F+N++  +++FE L+ IA Y+KS++++  P+NNK+AK+G
Sbjct:    3 LHYWDIRGLAQPIRLLLEYTGVKYEDKFYSCGEAPDYDKSCWFNEKPKLKMDFPNLPYLEDGDRKISQSNAIMRYIGRKHNLCGETEDEKVRVDILENQAMDFRNGFVMLCYL-NYQEKKAGYLEALPGVLKQFSDFLGDRKWFAGDKITFVDFIMYELLDQHRMFEPKCLDNFKNLQDLLDRFEALEKIAAYMKSSRFMKTPVNNKMAKWG 213          
BLAST of Glutathione S-transferase vs. Ensembl Cavefish
Match: ENSAMXT00000048694.1 (glutathione S-transferase Mu 3 [Source:NCBI gene;Acc:103025109])

HSP 1 Score: 223.787 bits (569), Expect = 5.376e-72
Identity = 102/212 (48.11%), Postives = 153/212 (72.17%), Query Frame = 3
Query:  147 LGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFG 782
            L YW IRGLAQPIRLLLEYTG +Y++K Y  G APD+++  W NEK  L + FPNLPYL +GD K++QS+AI+RYI  KH++ G + +E+  V+++ ++  D R  FV LCY  ++ + K  Y+  LP  LK FSDFLG ++W  G+K+++ DF  Y++LD  +M  P CLD+F+N++  +++FE L+ IA Y+KS++++  P+NNK+AK+G
Sbjct:    3 LHYWDIRGLAQPIRLLLEYTGVKYEDKFYSCGEAPDYDKSCWFNEKPKLKMDFPNLPYLEDGDRKISQSNAIMRYIGRKHNLCGETEDEKVRVDILENQAMDFRNGFVMLCYL-NYQEKKAGYLEALPGVLKQFSDFLGDRKWFAGDKITFVDFIMYELLDQHRMFEPKCLDNFKNLQDLLDRFEALEKIAAYMKSSRFMKTPVNNKMAKWG 213          
BLAST of Glutathione S-transferase vs. Ensembl Cavefish
Match: GSTM3 (glutathione S-transferase Mu 3-like [Source:NCBI gene;Acc:103025412])

HSP 1 Score: 218.779 bits (556), Expect = 5.031e-70
Identity = 99/216 (45.83%), Postives = 149/216 (68.98%), Query Frame = 3
Query:  135 MAPMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFG 782
            M   L YW IRG AQ IRLLLEYTG +Y+ K Y  G APD++R  W N+K  L + F NLPYL +GD K+ QS+AI+RYIA K+++ G + +E+  V+++ ++  D R  F+RLCY       K  Y+  LP  LK FSDFLG ++W  G+K+++ DF  Y++LD  +M  P CLD+F+N++ ++++FE L+ I+ Y+KS++++  P+NNK+AK+G
Sbjct:    9 MTMKLYYWDIRGAAQHIRLLLEYTGAKYENKFYSCGEAPDYDRSSWFNDKAKLKMDFTNLPYLEDGDRKIPQSNAIMRYIARKYNLCGETEDEKVRVDILENQAMDFRNGFIRLCYMA--YDNKSAYLEALPGVLKQFSDFLGGRKWFAGDKITFVDFTMYELLDQHRMFEPKCLDNFKNLRDFLDRFEALEKISAYMKSSRFMKTPVNNKMAKWG 222          
BLAST of Glutathione S-transferase vs. Ensembl Cavefish
Match: ENSAMXT00000048867.1 (glutathione S-transferase Mu 3 [Source:NCBI gene;Acc:103025109])

HSP 1 Score: 210.69 bits (535), Expect = 3.214e-66
Identity = 96/204 (47.06%), Postives = 147/204 (72.06%), Query Frame = 3
Query:  171 LAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFG 782
            LAQPIRLLLEYTG +Y++K Y  G APD+++  W NEK  L + FPNLPYL +GD K++QS+AI+RYI  KH++ G + +E+  V+++ ++  D R  FV LCY  ++ + K  Y+  LP  LK FSDFLG ++W  G+K+++ DF  Y++LD  +M  P CLD+F+N++  +++FE L+ IA Y+KS++++  P+NNK+AK+G
Sbjct:   65 LAQPIRLLLEYTGVKYEDKFYSCGEAPDYDKSCWFNEKPKLKMDFPNLPYLEDGDRKISQSNAIMRYIGRKHNLCGETEDEKVRVDILENQAMDFRNGFVMLCYL-NYQEKKAGYLEALPGVLKQFSDFLGDRKWFAGDKITFVDFIMYELLDQHRMFEPKCLDNFKNLQDLLDRFEALEKIAAYMKSSRFMKTPVNNKMAKWG 267          
BLAST of Glutathione S-transferase vs. Ensembl Cavefish
Match: GSTP1 (glutathione S-transferase pi 1 [Source:NCBI gene;Acc:103038276])

HSP 1 Score: 102.449 bits (254), Expect = 1.967e-25
Identity = 59/213 (27.70%), Postives = 106/213 (49.77%), Query Frame = 3
Query:  135 MAP-MLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQ--WLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNN 764
            MAP  + Y+ +RG     R++L   G+E++E +           ++W+         F  LP   +G   L QS+  LR++  KH   G +  E A ++M  D ++DLR  ++++ Y  ++   KD YI  LP+ L  F   L   +  +L+G+ +S+ D++ +++L    +LSP CLD    +K++++       I  +L S  Y   P+N 
Sbjct:    1 MAPYTITYFAVRGRCGATRIMLADQGQEWKEIVVSF--------EDWMKGDLKKTCVFGQLPKFEDGGLTLFQSNTFLRHLGRKHGAYGKNDCEAAQIDMFNDGVEDLRTKYLKMIYQ-EYETGKDAYIKDLPNHLCKFEAVLAKNKSGFLVGDSISFADYSLFEVLLNHLVLSPTCLDSLPALKSFVDTLSARPKIKAFLDSEDYKKLPING 204          
BLAST of Glutathione S-transferase vs. Ensembl Nematostella
Match: EDO42590 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S183])

HSP 1 Score: 214.542 bits (545), Expect = 6.795e-69
Identity = 106/220 (48.18%), Postives = 150/220 (68.18%), Query Frame = 3
Query:  135 MAPMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGD---FKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYS-PDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFG 782
            M  + GYW IRGLAQPIRLLL+Y GEE+++K Y+       +R  W NEKFSLGL FPNLPY I+GD    K+TQS+AILRYI  K+DM G + EE+ IV+++ +++ D R  FVRLCY+   F + K  YI    S +K F+DFLG K +L G+ L++ DF  Y++LD  ++  P+ LD   N+K ++++FEKL  IA Y+ SN++I +P+N+ +A F 
Sbjct:    1 MPSVFGYWAIRGLAQPIRLLLKYNGEEFEDKRYD-----GHDRTSWTNEKFSLGLDFPNLPYYIDGDKKHIKITQSNAILRYIGRKYDMCGKTEEEKVIVDILENQLMDFRNGFVRLCYNNAAFEENKPAYIQSAQSSIKAFADFLGQKSYLAGDNLTFVDFVLYELLDQHRIFEPSLLDAHPNLKAFLDRFEKLPAIAAYMSSNEFIKRPINSPIAAFA 215          
BLAST of Glutathione S-transferase vs. Ensembl Nematostella
Match: EDO47273 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RMI2])

HSP 1 Score: 202.601 bits (514), Expect = 3.092e-64
Identity = 95/214 (44.39%), Postives = 148/214 (69.16%), Query Frame = 3
Query:  138 APMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKF 779
             P+LGYW IRGL QPIRLLL+YT E++++K Y L +     ++EW  EK++LGL FPNLPY I+GD  +TQS+AILRYIA +H+M G + +E  IV+M+ +++ ++   F R C+ P   K K  Y+  + + +K F+DFLG K +L GE+L++ DF  Y+++D  ++  P+ L   EN+K ++N+ E+L  +A+Y+KS ++IH+P+N   A F
Sbjct:    4 VPILGYWPIRGLGQPIRLLLKYTNEDFEDKRYALASGG--KQNEWFIEKYNLGLDFPNLPYFIDGDIMITQSNAILRYIARRHNMCGKTKKEMVIVDMLENQVMNI---FYR-CFKPTIFKNKAVYLKDIKAVIKRFADFLGEKTYLAGEELTFVDFVMYELMDHHRLFEPSLLQPHENLKMFLNRIEQLPAVAEYMKSEEFIHRPINAPFASF 211          
BLAST of Glutathione S-transferase vs. Ensembl Nematostella
Match: EDO42589 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S182])

HSP 1 Score: 192.2 bits (487), Expect = 4.538e-60
Identity = 90/217 (41.47%), Postives = 147/217 (67.74%), Query Frame = 3
Query:  135 MAPMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCY-SPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFG 782
            MAP+ GYWK+RGLA+ +R LL YT  EY+ K Y  G+APDFN+D WL+EK ++GL  PN+PY ++GD KLTQ+SAI+R+IA +HD+ G + EE+ + +M+  EI+D    F +  Y + +F +    Y+  + + +K F++FLG+  +  G+ L++ DFN ++ LD ++ L P  LD  +N+K ++ + ++L  + +Y+ S  Y   P++NK+AK+G
Sbjct:    1 MAPIFGYWKLRGLAEGVRYLLAYTNTEYERKDYVTGDAPDFNKDGWLSEKENIGLLLPNVPYNMDGDIKLTQTSAIMRHIARQHDLCGKTEEEKCLCDMIDYEIKDFFPGFFKATYFTNNFEEDIKVYLKGIRTLVKRFANFLGTNPYFAGQNLTFADFNVWEALDQLRTLDPTILDGHDNLKGFMKRIQELPGVREYMASEFYHSLPISNKMAKWG 217          
BLAST of Glutathione S-transferase vs. Ensembl Nematostella
Match: EDO38608 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SCI4])

HSP 1 Score: 67.3958 bits (163), Expect = 2.967e-13
Identity = 50/169 (29.59%), Postives = 84/169 (49.70%), Query Frame = 3
Query:  147 LGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGD-FKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPD----FIKLKDDY-ISQLPSRLKLFSDFL--GSKQWLIGEKLSYPDFNFYDILD 629
            L Y+  RG A+P RL     G EY++K +E        R+EWL  K  L   F  +P L+  D  KL QS AIL Y+A +  +  ++  + A+ +M+     DL    ++     D     +KL+++   + +P+ L+  S FL      +L+ +KL+Y D   +++ D
Sbjct:    6 LHYFNARGRAEPARLAFAAAGIEYEDKRFE-------GREEWLRVKPELDPPFGQVPLLVIDDKIKLAQSMAILAYVAREGGLAPTNSLDIAMCDMIVGVEGDLNSKGMKWFMEKDEKKKVLKLREELDTTHIPAFLEAMSKFLEKNGTGFLVTDKLTYADILVFNVCD 167          
BLAST of Glutathione S-transferase vs. Ensembl Nematostella
Match: EDO33767 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SR87])

HSP 1 Score: 63.1586 bits (152), Expect = 1.074e-11
Identity = 63/217 (29.03%), Postives = 95/217 (43.78%), Query Frame = 3
Query:  135 MAPM--LGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYL-IEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDF----IKLKDDYISQLPSRL----KLFSDFLGSKQWLIGEKLSYPDFNFYDILDGI----KMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNK 740
            MAP   + Y+  R  A+ IR+LL      + +   E    PD     W   K S    F  LP L I    KL QS AI R++A++H +  S    +A  +M  D  +D+        +  D      K K+ +   LP RL    KLF +  G K +L+G+K++Y D +F+   +G     K+  P  L  +  +    N+      I +YLK  K
Sbjct:    1 MAPTYRVVYFDARARAECIRVLLHLADVPFTD---ERVAPPD-----WAAMKTSGRCPFGELPLLEISDGRKLAQSHAIFRFLAKEHGLAPSDSYSQARCDMFLDNTKDMIEKVGTAFWEKDEKLKEQKSKEFHEETLPKRLEFINKLFQENKGGKGYLVGDKITYADIDFFCFFNGFINSGKLDVPEQLSKYPLLVDLYNRVMNEKKILEYLKQRK 209          
BLAST of Glutathione S-transferase vs. Ensembl Medaka
Match: gstm.1 (glutathione S-transferase Mu 3 [Source:NCBI gene;Acc:101157367])

HSP 1 Score: 239.965 bits (611), Expect = 1.784e-77
Identity = 107/216 (49.54%), Postives = 158/216 (73.15%), Query Frame = 3
Query:  135 MAPMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFG 782
            MA  L YW IRGLAQPIRLLLEYTG  Y++K Y  G AP++++  W + K  LG+ FPNLPYL++GD K+ QS+AI+RYIA KH++ G + +E+  V+++ ++  D R  FVR+CY+ D  K+K  Y+  LP  LK FSDFLG + W  GEK+++ DF  Y++LD  +M  P+CLDDF+N+K ++++FE L+ I+ Y+KS++++  P+NNK+AK+G
Sbjct:   66 MAMTLAYWDIRGLAQPIRLLLEYTGTSYEDKFYVCGEAPNYDKSCWFDIKEKLGMDFPNLPYLLDGDRKIVQSNAIMRYIARKHNLCGETEDEKVRVDIIENQAMDFRNGFVRMCYT-DLDKMKPGYLKSLPEVLKQFSDFLGDRDWFAGEKITFVDFLMYELLDQHRMFHPSCLDDFKNLKDFLDRFEALEKISAYMKSDRFMKTPVNNKMAKWG 280          
BLAST of Glutathione S-transferase vs. Ensembl Medaka
Match: GSTM4 (glutathione S-transferase Mu 5 [Source:NCBI gene;Acc:101157858])

HSP 1 Score: 234.958 bits (598), Expect = 1.904e-76
Identity = 104/216 (48.15%), Postives = 159/216 (73.61%), Query Frame = 3
Query:  135 MAPMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFG 782
            M+  LGYW IRGLA P RLLLEYTG +Y+EK Y  G AP++++  W + KF LGL FPNLPYL++GD K+ QS+AI RY+A K+ + G + EE+  ++++ + + D RMSF+R+CY+ D+  LK +Y+  LP  LK FSDFLG+++W  G++L++ DF  YD+LD  ++  P CLD F+N+K ++++FE L+ I+ ++KS +YI  P++N++AK+G
Sbjct:    1 MSMTLGYWDIRGLAHPTRLLLEYTGAKYEEKTYVCGEAPNYDKSGWFSVKFKLGLDFPNLPYLVDGDKKIVQSNAIQRYLARKNKLCGETEEEKIRLDILENVVMDFRMSFIRMCYT-DYENLKPEYLKTLPDVLKQFSDFLGNRKWFAGDRLTFGDFIMYDVLDQQRIFLPTCLDAFKNLKDFLDRFESLERISAFMKSGRYIRTPIHNRMAKWG 215          
BLAST of Glutathione S-transferase vs. Ensembl Medaka
Match: GSTM4 (glutathione S-transferase Mu 5 [Source:NCBI gene;Acc:101157858])

HSP 1 Score: 219.164 bits (557), Expect = 1.114e-69
Identity = 98/216 (45.37%), Postives = 155/216 (71.76%), Query Frame = 3
Query:  135 MAPMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFG 782
            +  +L Y +   LA P RLLLEYTG +Y+EK Y  G AP++++  W + KF LGL FPNLPYL++GD K+ QS+AI RY+A K+ + G + EE+  ++++ + + D RMSF+R+CY+ D+  LK +Y+  LP  LK FSDFLG+++W  G++L++ DF  YD+LD  ++  P CLD F+N+K ++++FE L+ I+ ++KS +YI  P++N++AK+G
Sbjct:   36 VTKLLSYSEPVQLAHPTRLLLEYTGAKYEEKTYVCGEAPNYDKSGWFSVKFKLGLDFPNLPYLVDGDKKIVQSNAIQRYLARKNKLCGETEEEKIRLDILENVVMDFRMSFIRMCYT-DYENLKPEYLKTLPDVLKQFSDFLGNRKWFAGDRLTFGDFIMYDVLDQQRIFLPTCLDAFKNLKDFLDRFESLERISAFMKSGRYIRTPIHNRMAKWG 250          
BLAST of Glutathione S-transferase vs. Ensembl Medaka
Match: ENSORLT00000012133.2 (glutathione S-transferase alpha 4 [Source:NCBI gene;Acc:101174370])

HSP 1 Score: 74.7146 bits (182), Expect = 2.945e-15
Identity = 57/197 (28.93%), Postives = 96/197 (48.73%), Query Frame = 3
Query:  144 MLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSR-LKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLK 731
            +L Y+  RG  + IR LL     E+ E           +RD++        L F  +P +     KL Q+ AIL YIAEK+++ G  ++ERA+VNM A+ + DL    + L +  D     D+  S+   R L +F   L    +L+G KLSY D    +    ++      L DF ++K++  +  K+  ++++L+
Sbjct:    6 VLHYFNGRGKMESIRWLLTVVEAEFDEMYLT-------SRDQYEKLLNDGSLMFQQVPLVEIDGMKLVQTKAILHYIAEKYNLHGKDIKERAMVNMYAEGLIDLMEMMMMLPFVADPKPKLDNIQSKAKERYLPVFEKALSGPVYLVGGKLSYADVLLLECTLMLEEKFTGILGDFPSVKSFQGRMIKIPAVSRFLQ 195          
BLAST of Glutathione S-transferase vs. Planmine SMEST
Match: SMESG000015099.1 (SMESG000015099.1)

HSP 1 Score: 444.121 bits (1141), Expect = 6.791e-159
Identity = 218/220 (99.09%), Postives = 218/220 (99.09%), Query Frame = 3
Query:  135 MAPMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFGGDTD 794
            MAPMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGD KLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWL GEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFGGDTD
Sbjct:    1 MAPMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDLKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLNGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFGGDTD 220          
BLAST of Glutathione S-transferase vs. Planmine SMEST
Match: SMESG000015099.1 (SMESG000015099.1)

HSP 1 Score: 437.187 bits (1123), Expect = 3.069e-156
Identity = 215/217 (99.08%), Postives = 215/217 (99.08%), Query Frame = 3
Query:  144 MLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFGGDTD 794
            MLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGD KLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWL GEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFGGDTD
Sbjct:    1 MLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDLKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLNGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFGGDTD 217          
BLAST of Glutathione S-transferase vs. Planmine SMEST
Match: SMESG000014182.1 (SMESG000014182.1)

HSP 1 Score: 425.246 bits (1092), Expect = 2.063e-151
Identity = 208/220 (94.55%), Postives = 213/220 (96.82%), Query Frame = 3
Query:  135 MAPMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFGGDTD 794
            MAPMLGYWK+RGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSF RLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDF FYDILDGIKMLSPNCL+DFENIKTY+ +FEKLDPIAKYLKSNKYI KPL +K A FGGDTD
Sbjct:    1 MAPMLGYWKLRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFARLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFIFYDILDGIKMLSPNCLNDFENIKTYMIRFEKLDPIAKYLKSNKYIRKPLYSKAASFGGDTD 220          
BLAST of Glutathione S-transferase vs. Planmine SMEST
Match: SMESG000014182.1 (SMESG000014182.1)

HSP 1 Score: 417.927 bits (1073), Expect = 1.224e-148
Identity = 205/217 (94.47%), Postives = 210/217 (96.77%), Query Frame = 3
Query:  144 MLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFGGDTD 794
            MLGYWK+RGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSF RLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDF FYDILDGIKMLSPNCL+DFENIKTY+ +FEKLDPIAKYLKSNKYI KPL +K A FGGDTD
Sbjct:    1 MLGYWKLRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFARLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFIFYDILDGIKMLSPNCLNDFENIKTYMIRFEKLDPIAKYLKSNKYIRKPLYSKAASFGGDTD 217          
BLAST of Glutathione S-transferase vs. Planmine SMEST
Match: SMESG000015094.1 (SMESG000015094.1)

HSP 1 Score: 412.535 bits (1059), Expect = 1.718e-146
Identity = 204/220 (92.73%), Postives = 211/220 (95.91%), Query Frame = 3
Query:  135 MAPMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEKFSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMVADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIGEKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKSNKYIHKPLNNKVAKFGGDTD 794
            MAPMLGYW+IRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNR+EWLNEKFSLGLSFPNLPYLIEGD KLTQSSAILRYIAEKHDMVGSSV++RAIVNMVADEIQDLR+S  +LCYSPDFIKLKDDYISQLP RLKLFSDFLGSKQWLI EKL+YPDFNFYDILDGIK LSPNCLDDFENIKTYI KFEKLDPIAKYLKSNKYIHKPLN  VAKFGGDTD
Sbjct:    1 MAPMLGYWRIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRNEWLNEKFSLGLSFPNLPYLIEGDLKLTQSSAILRYIAEKHDMVGSSVKKRAIVNMVADEIQDLRISLGKLCYSPDFIKLKDDYISQLPKRLKLFSDFLGSKQWLIDEKLNYPDFNFYDILDGIKKLSPNCLDDFENIKTYIYKFEKLDPIAKYLKSNKYIHKPLNT-VAKFGGDTD 219          
The following BLAST results are available for this feature:
BLAST of Glutathione S-transferase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
GSTM42.676e-8051.39glutathione S-transferase mu 4 [Source:HGNC Symbol... [more]
GSTM42.738e-7950.46glutathione S-transferase mu 4 [Source:HGNC Symbol... [more]
GSTM32.463e-7851.87glutathione S-transferase mu 3 [Source:HGNC Symbol... [more]
GSTM32.463e-7851.87glutathione S-transferase mu 3 [Source:HGNC Symbol... [more]
GSTM22.830e-7850.93glutathione S-transferase mu 2 [Source:HGNC Symbol... [more]
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BLAST of Glutathione S-transferase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
gst-11.988e-2129.77Glutathione S-transferase P [Source:UniProtKB/Swi... [more]
gst-11.988e-2129.77Glutathione S-transferase P [Source:UniProtKB/Swi... [more]
gst-234.750e-2132.02Glutathione S-Transferase [Source:UniProtKB/TrEMB... [more]
gst-255.188e-1926.98Glutathione S-Transferase [Source:UniProtKB/TrEMB... [more]
gst-107.596e-1927.45Glutathione S-transferase P 10 [Source:UniProtKB/... [more]
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BLAST of Glutathione S-transferase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 4
Match NameE-valueIdentityDescription
GstS14.658e-922.77gene:FBgn0010226 transcript:FBtr0087005[more]
GstS14.658e-922.77gene:FBgn0010226 transcript:FBtr0100258[more]
GstS14.658e-922.77gene:FBgn0010226 transcript:FBtr0087006[more]
GstS17.812e-823.15gene:FBgn0010226 transcript:FBtr0333551[more]
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BLAST of Glutathione S-transferase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
gstm.38.598e-8051.85glutathione S-transferase mu tandem duplicate 3 [S... [more]
gstm.12.494e-7951.39glutathione S-transferase mu, tandem duplicate 1 [... [more]
gstm.26.558e-7951.85glutathione S-transferase mu tandem duplicate 2 [S... [more]
gstm.31.996e-4253.72glutathione S-transferase mu tandem duplicate 3 [S... [more]
gstp11.557e-3028.17glutathione S-transferase pi 1 [Source:ZFIN;Acc:ZD... [more]
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BLAST of Glutathione S-transferase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
krt8.22.414e-7047.42keratin 8 [Source:Xenbase;Acc:XB-GENE-876686][more]
nemp11.228e-3033.65nuclear envelope integral membrane protein 1 [Sour... [more]
gnrhr1.657e-2933.78gonadotropin releasing hormone receptor [Source:Xe... [more]
gnrhr3.950e-2934.27gonadotropin releasing hormone receptor [Source:Xe... [more]
gnrhr5.850e-2633.80gonadotropin releasing hormone receptor [Source:Xe... [more]
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BLAST of Glutathione S-transferase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Gstm43.017e-8252.78glutathione S-transferase, mu 4 [Source:MGI Symbol... [more]
Gstm11.721e-8050.46glutathione S-transferase, mu 1 [Source:MGI Symbol... [more]
Gstm51.765e-7751.40glutathione S-transferase, mu 5 [Source:MGI Symbol... [more]
Gstm28.059e-7750.23glutathione S-transferase, mu 2 [Source:MGI Symbol... [more]
Gstm31.012e-7647.71glutathione S-transferase, mu 3 [Source:MGI Symbol... [more]
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BLAST of Glutathione S-transferase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9N0V4|GSTM1_BOVIN8.486e-8152.31Glutathione S-transferase Mu 1 OS=Bos taurus OX=99... [more]
sp|P10649|GSTM1_MOUSE1.204e-7950.46Glutathione S-transferase Mu 1 OS=Mus musculus OX=... [more]
sp|Q03013|GSTM4_HUMAN1.285e-7951.39Glutathione S-transferase Mu 4 OS=Homo sapiens OX=... [more]
sp|P16413|GSTMU_CAVPO4.109e-7953.30Glutathione S-transferase B OS=Cavia porcellus OX=... [more]
sp|Q9BEB0|GSTM2_MACFU6.145e-7951.39Glutathione S-transferase Mu 2 OS=Macaca fuscata f... [more]
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BLAST of Glutathione S-transferase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
G9BRW21.585e-12076.26Glutathione S-transferase OS=Dugesia japonica OX=6... [more]
A0A151N8K84.433e-8856.48Glutathione S-transferase Mu 3 OS=Alligator missis... [more]
F8TTE31.366e-8253.74Glutathione S-transferase mu 3 OS=Meleagris gallop... [more]
I1SZ682.257e-8254.38Glutathione S-transferase 1 OS=Spirometra erinacei... [more]
A0A098LZU63.026e-8253.24Glutathione S-transferase Mu 1-like protein OS=Hyp... [more]
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BLAST of Glutathione S-transferase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000030565.15.376e-7248.11glutathione S-transferase Mu 3 [Source:NCBI gene;A... [more]
ENSAMXT00000048694.15.376e-7248.11glutathione S-transferase Mu 3 [Source:NCBI gene;A... [more]
GSTM35.031e-7045.83glutathione S-transferase Mu 3-like [Source:NCBI g... [more]
ENSAMXT00000048867.13.214e-6647.06glutathione S-transferase Mu 3 [Source:NCBI gene;A... [more]
GSTP11.967e-2527.70glutathione S-transferase pi 1 [Source:NCBI gene;A... [more]
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BLAST of Glutathione S-transferase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Glutathione S-transferase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Glutathione S-transferase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO425906.795e-6948.18Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO472733.092e-6444.39Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO425894.538e-6041.47Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO386082.967e-1329.59Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO337671.074e-1129.03Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Glutathione S-transferase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 4
Match NameE-valueIdentityDescription
gstm.11.784e-7749.54glutathione S-transferase Mu 3 [Source:NCBI gene;A... [more]
GSTM41.904e-7648.15glutathione S-transferase Mu 5 [Source:NCBI gene;A... [more]
GSTM41.114e-6945.37glutathione S-transferase Mu 5 [Source:NCBI gene;A... [more]
ENSORLT00000012133.22.945e-1528.93glutathione S-transferase alpha 4 [Source:NCBI gen... [more]
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BLAST of Glutathione S-transferase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000015099.16.791e-15999.09SMESG000015099.1[more]
SMESG000015099.13.069e-15699.08SMESG000015099.1[more]
SMESG000014182.12.063e-15194.55SMESG000014182.1[more]
SMESG000014182.11.224e-14894.47SMESG000014182.1[more]
SMESG000015094.11.718e-14692.73SMESG000015094.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30000883 ID=SMED30000883|Name=Glutathione S-transferase|organism=Schmidtea mediterranea sexual|type=transcript|length=1042bp
GTAACTTCAAAAAATATAAGAAAACTGCTTTTATAGCTTATGTATTTTTT
TAATGAAAATTCGTTTTAATATATTTGTGTAATTTTAATTCCTTAAAATC
AATCAACTTTCCAATATTATTGTATTTGTTTAACATGGCTCCTATGTTAG
GTTACTGGAAGATTCGAGGACTAGCTCAACCTATTAGACTTTTACTGGAA
TATACAGGAGAAGAATACCAAGAAAAGTTATACGAATTGGGGAACGCGCC
GGATTTCAATAGAGATGAATGGTTAAATGAAAAATTTAGTTTAGGATTAA
GTTTTCCAAATCTGCCATATTTAATTGAAGGAGATTTTAAATTAACTCAA
TCTTCTGCTATTTTGCGTTACATAGCAGAAAAGCATGACATGGTTGGATC
TTCTGTTGAAGAAAGAGCTATCGTAAATATGGTCGCAGATGAAATCCAGG
ATCTTCGAATGAGTTTTGTAAGATTGTGTTATAGCCCGGATTTTATCAAA
TTAAAAGATGATTATATATCGCAATTACCAAGTCGTTTGAAATTATTTTC
CGACTTTTTAGGAAGTAAACAATGGCTGATTGGTGAAAAGTTAAGTTATC
CCGACTTTAATTTCTATGACATACTAGACGGCATAAAAATGCTTTCACCA
AATTGTTTGGACGATTTTGAAAATATCAAAACATATATAAATAAATTTGA
GAAATTGGATCCGATTGCGAAGTATTTGAAGTCTAATAAATATATACACA
AACCGTTGAATAATAAAGTCGCAAAATTTGGAGGTGATACCGATTAAATA
TTATCGGTAAAATGTATATCAAACTTGCCTTGATTTATGCTTATTAATAA
ATTTGATTAATTTTTAATTAAATCAAAAAAAGTTGTTCTTTTGCATGATT
ATTATAATACATGCTAAGATAAATGTTTTTACGAGATTTATCTTGATTGA
TTGATTATGTGCATCCTCAAAATATTAACATTCAGACCTCCATTGAATAT
ATTATTATATTTTAGCAAGGCTATTTAAATAAAAAAAATCGA
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protein sequence of SMED30000883-orf-1

>SMED30000883-orf-1 ID=SMED30000883-orf-1|Name=SMED30000883-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=221bp
MAPMLGYWKIRGLAQPIRLLLEYTGEEYQEKLYELGNAPDFNRDEWLNEK
FSLGLSFPNLPYLIEGDFKLTQSSAILRYIAEKHDMVGSSVEERAIVNMV
ADEIQDLRMSFVRLCYSPDFIKLKDDYISQLPSRLKLFSDFLGSKQWLIG
EKLSYPDFNFYDILDGIKMLSPNCLDDFENIKTYINKFEKLDPIAKYLKS
NKYIHKPLNNKVAKFGGDTD*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR036249Thioredoxin-like_sf
IPR040079Glutathione_S-Trfase
IPR036282Glutathione-S-Trfase_C_sf
IPR003081GST_mu
IPR010987Glutathione-S-Trfase_C-like
IPR004046GST_C
IPR012336Thioredoxin-like_fold
IPR004045Glutathione_S-Trfase_N
Vocabulary: biological process
TermDefinition
GO:0008152metabolic process
Vocabulary: molecular function
TermDefinition
GO:0004364glutathione transferase activity
GO:0005515protein binding
GO:0016740transferase activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003081Glutathione S-transferase, Mu classPRINTSPR01267GSTRNSFRASEMcoord: 31..43
score: 53.15
coord: 44..56
score: 61.89
coord: 139..152
score: 50.97
NoneNo IPR availableGENE3DG3DSA:1.20.1050.10coord: 87..191
e-value: 8.4E-86
score: 288.8
NoneNo IPR availableSFLDSFLDG01205AMPS.1coord: 5..200
e-value: 0.0
score: 186.2
NoneNo IPR availablePANTHERPTHR11571GLUTATHIONE S-TRANSFERASEcoord: 2..213
NoneNo IPR availablePANTHERPTHR11571:SF137GLUTATHIONE S-TRANSFERASE MU 1coord: 2..213
NoneNo IPR availableCDDcd03075GST_N_Mucoord: 3..84
e-value: 3.43455E-48
score: 151.0
IPR004046Glutathione S-transferase, C-terminalPFAMPF14497GST_C_3coord: 106..200
e-value: 1.3E-14
score: 54.2
IPR004045Glutathione S-transferase, N-terminalPFAMPF02798GST_Ncoord: 5..82
e-value: 3.0E-17
score: 62.7
IPR004045Glutathione S-transferase, N-terminalPROSITEPS50404GST_NTERcoord: 1..88
score: 27.885
IPR036249Thioredoxin-like superfamilyGENE3DG3DSA:3.40.30.10coord: 5..208
e-value: 8.4E-86
score: 288.8
IPR036249Thioredoxin-like superfamilySUPERFAMILYSSF52833Thioredoxin-likecoord: 5..85
IPR040079Glutathione Transferase familySFLDSFLDS00019Glutathione_Transferase_(cytocoord: 5..200
e-value: 0.0
score: 186.2
IPR010987Glutathione S-transferase, C-terminal-likePROSITEPS50405GST_CTERcoord: 90..208
score: 20.843
IPR036282Glutathione S-transferase, C-terminal domain superfamilySUPERFAMILYSSF47616GST C-terminal domain-likecoord: 87..217