Caltractin

Overview
NameCaltractin
Smed IDSMED30000846
Length (bp)849
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Caltractin (SMED30000846) t-SNE clustered cells

Violin plots show distribution of expression levels for Caltractin (SMED30000846) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Caltractin (SMED30000846) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Caltractin (SMED30000846) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30000846

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30000846SMESG000029908.1 SmedASXL_009616SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
pharynxSMED30000846SMESG000029908.1 dd_Smed_v4_8753_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30000846SMESG000029908.1 dd_Smed_v4_8753_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30000846SMESG000029908.1 dd_Smed_v4_8753_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30000846SMESG000029908.1 dd_Smed_v6_8753_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
epidermisSMED30000846SMESG000029908.1 dd_Smed_v4_8753_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Caltractin vs. Ensembl Human
Match: CETN2 (centrin 2 [Source:HGNC Symbol;Acc:HGNC:1867])

HSP 1 Score: 113.62 bits (283), Expect = 1.987e-30
Identity = 62/165 (37.58%), Postives = 107/165 (64.85%), Query Frame = 3
Query:  267 NMVISSKALK-----ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNLF 743
            NM  SS+  +     EL  E+ ++++  FD+FD++  G + + EL++++RALGF+    + ++++ E+   G   ++F  FL  + +   E +DT EE+++ FK FD  +TG IS  NLK ++ EL   L+  +LQ+MI+EAD +GDG VSE+EF++IM +T+L+
Sbjct:    9 NMASSSQRKRMSPKPELTEEQKQEIREAFDLFDADGTGTIDVKELKVAMRALGFEPKKEEIKKMISEIDKEGTGKMNFGDFLTVMTQKMSE-KDTKEEILKAFKLFDDDETGKISFKNLKRVAKELGENLTDEELQEMIDEADRDGDGEVSEQEFLRIMKKTSLY 172          
BLAST of Caltractin vs. Ensembl Human
Match: CETN1 (centrin 1 [Source:HGNC Symbol;Acc:HGNC:1866])

HSP 1 Score: 109.383 bits (272), Expect = 9.552e-29
Identity = 58/150 (38.67%), Postives = 99/150 (66.00%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNLF 743
            EL  ++ ++++  FD+FD +  G +   EL++++RALGF+    + ++++ E+   G   I FN FL  + +   E +DT EE+++ F+ FD  +TG IS  NLK ++ EL   L+  +LQ+MI+EAD +GDG V+EEEF++IM +T+L+
Sbjct:   24 ELTEDQKQEVREAFDLFDVDGSGTIDAKELKVAMRALGFEPRKEEMKKMISEVDREGTGKISFNDFLAVMTQKMSE-KDTKEEILKAFRLFDDDETGKISFKNLKRVANELGENLTDEELQEMIDEADRDGDGEVNEEEFLRIMKKTSLY 172          
BLAST of Caltractin vs. Ensembl Human
Match: CETN3 (centrin 3 [Source:HGNC Symbol;Acc:HGNC:1868])

HSP 1 Score: 92.4337 bits (228), Expect = 2.294e-22
Identity = 55/155 (35.48%), Postives = 93/155 (60.00%), Query Frame = 3
Query:  279 SSKALKELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELME----LSTNGIKIIDFNGFL-NCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMT 728
              K  +EL  E+ +++K  F++FD++ D  +   EL++++RALGF    A   ++++     +T  I   DFN  + + ++E     RD  EE+++ FK FD  D+G ISL NL+ ++ EL   +S  +L+ MI E D +GDG +++EEF+ IMT
Sbjct:   15 KRKKRRELSEEQKQEIKDAFELFDTDKDEAIDYHELKVAMRALGFDVKKADVLKILKDYDREATGKITFEDFNEVVTDWILE-----RDPHEEILKAFKLFDDDDSGKISLRNLRRVARELGENMSDEELRAMIEEFDKDGDGEINQEEFIAIMT 164          
BLAST of Caltractin vs. Ensembl Human
Match: CALM1 (calmodulin 1 [Source:HGNC Symbol;Acc:HGNC:1442])

HSP 1 Score: 88.1965 bits (217), Expect = 4.685e-21
Identity = 51/145 (35.17%), Postives = 86/145 (59.31%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMT 728
            +L  E+I + K  F +FD + DG ++  EL   +R+LG   + A+ ++++ E+  +G   IDF  FL  +    K++ D+ EE+ + F+ FD    G+IS   L+ +   L   L+  ++ +MI EADI+GDG V+ EEFV++MT
Sbjct:    5 QLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDT-DSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMT 148          
BLAST of Caltractin vs. Ensembl Human
Match: CALM2 (calmodulin 2 [Source:HGNC Symbol;Acc:HGNC:1445])

HSP 1 Score: 88.1965 bits (217), Expect = 4.964e-21
Identity = 51/145 (35.17%), Postives = 86/145 (59.31%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMT 728
            +L  E+I + K  F +FD + DG ++  EL   +R+LG   + A+ ++++ E+  +G   IDF  FL  +    K++ D+ EE+ + F+ FD    G+IS   L+ +   L   L+  ++ +MI EADI+GDG V+ EEFV++MT
Sbjct:    4 QLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDT-DSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMT 147          
BLAST of Caltractin vs. Ensembl Celegans
Match: cmd-1 (Calmodulin [Source:UniProtKB/Swiss-Prot;Acc:O16305])

HSP 1 Score: 88.1965 bits (217), Expect = 1.816e-21
Identity = 52/145 (35.86%), Postives = 85/145 (58.62%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMT 728
            +L  E+I + K  F +FD + DG ++  EL   +R+LG   + A+ ++++ E+  +G   IDF  FL  +    K++ D+ EE+ + F+ FD    GFIS   L+ +   L   L+  ++ +MI EADI+GDG V+ EEFV +MT
Sbjct:    4 QLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDT-DSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVTMMT 147          
BLAST of Caltractin vs. Ensembl Celegans
Match: cal-1 (Calmodulin-like protein [Source:UniProtKB/Swiss-Prot;Acc:P04630])

HSP 1 Score: 74.7146 bits (182), Expect = 2.855e-16
Identity = 46/154 (29.87%), Postives = 82/154 (53.25%), Query Frame = 3
Query:  270 MVISSKALKELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMT 728
            M  S   +K+L PEEI + +  F +FD + +G +S  EL +++R+LG   +  +  E++ E+  +G   I+F  F  CV+  +       E + + F+ FD    G I+    +     + +  S  ++ +MI E D++GDG +  EEFVK+M+
Sbjct:    8 MQFSEDIIKQLTPEEIDEFREAFMMFDKDGNGTISTKELGIAMRSLGQNPTEQEILEMINEVDIDGNGQIEFPEF--CVMMKRMMKETDSEMIREAFRVFDKDGNGVITAQEFRYFMVHMGMQFSEEEVDEMIKEVDVDGDGEIDYEEFVKMMS 159          
BLAST of Caltractin vs. Ensembl Celegans
Match: cal-1 (Calmodulin-like protein [Source:UniProtKB/Swiss-Prot;Acc:P04630])

HSP 1 Score: 74.3294 bits (181), Expect = 5.996e-16
Identity = 46/154 (29.87%), Postives = 82/154 (53.25%), Query Frame = 3
Query:  270 MVISSKALKELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMT 728
            M  S   +K+L PEEI + +  F +FD + +G +S  EL +++R+LG   +  +  E++ E+  +G   I+F  F  CV+  +       E + + F+ FD    G I+    +     + +  S  ++ +MI E D++GDG +  EEFVK+M+
Sbjct:   27 MQFSEDIIKQLTPEEIDEFREAFMMFDKDGNGTISTKELGIAMRSLGQNPTEQEILEMINEVDIDGNGQIEFPEF--CVMMKRMMKETDSEMIREAFRVFDKDGNGVITAQEFRYFMVHMGMQFSEEEVDEMIKEVDVDGDGEIDYEEFVKMMS 178          
BLAST of Caltractin vs. Ensembl Celegans
Match: cmd-1 (Calmodulin [Source:UniProtKB/Swiss-Prot;Acc:O16305])

HSP 1 Score: 68.5514 bits (166), Expect = 1.464e-14
Identity = 41/112 (36.61%), Postives = 67/112 (59.82%), Query Frame = 3
Query:  396 LRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMT 728
            +R+LG   + A+ ++++ E+  +G   IDF  FL  +    K++ D+ EE+ + F+ FD    GFIS   L+ +   L   L+  ++ +MI EADI+GDG V+ EEFV +MT
Sbjct:    1 MRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDT-DSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVTMMT 111          
BLAST of Caltractin vs. Ensembl Celegans
Match: cal-8 (CALmodulin related genes [Source:UniProtKB/TrEMBL;Acc:Q09980])

HSP 1 Score: 65.855 bits (159), Expect = 3.708e-13
Identity = 46/139 (33.09%), Postives = 80/139 (57.55%), Query Frame = 3
Query:  321 DLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELMELST-NGIKIIDFNGFLNCV---IENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIM 725
            +++ VF  FD N DG ++  EL ++L  LG K S+++ E ++E +  +G   ID + FLN +   I + KE R    E+   F  FD +  G IS+D+L  +  +L   L+  + ++MI + D++ DG++  +EFV I+
Sbjct:    8 EIREVFREFDKNGDGRITRQELEVALLQLGEKASNSKIETMIEQADLDGNGCIDIDEFLNVLRRQICDPKEER----ELRDVFNVFDKNGDGVISIDDLIFVMCQLGEKLTETEAKEMIKQGDLDHDGMIDFQEFVNII 142          

HSP 2 Score: 45.8246 bits (107), Expect = 5.150e-6
Identity = 26/69 (37.68%), Postives = 44/69 (63.77%), Query Frame = 3
Query:  306 PEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM---ELSTNGIKIIDFNGFLNCV 503
            P+E ++L+ VF+VFD N DG +SI +L   +  LG K +  +A+E++   +L  +G  +IDF  F+N +
Sbjct:   76 PKEERELRDVFNVFDKNGDGVISIDDLIFVMCQLGEKLTETEAKEMIKQGDLDHDG--MIDFQEFVNII 142          
BLAST of Caltractin vs. Ensembl Fly
Match: CG17493 (gene:FBgn0040010 transcript:FBtr0332630)

HSP 1 Score: 102.064 bits (253), Expect = 4.011e-26
Identity = 54/142 (38.03%), Postives = 94/142 (66.20%), Query Frame = 3
Query:  321 DLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNLF 743
            D+K  FD+FD+   G++ + EL++++RALGF+    + + ++ ++  +    I FN FL  +     E +DT EE+++ F+ FD  DTG IS  NLK ++ EL   L+  +L++MI+EAD++ DG V++EEF++IM +T+L+
Sbjct:   42 DIKEAFDLFDNEGTGYIEVKELKVAIRALGFEPKKEEIKRMISDIDKDCSGRIAFNVFLQLMTIKMAE-KDTKEEILKAFRLFDDDDTGKISFRNLKRVARELGETLTDEELREMIDEADLDNDGEVNQEEFLRIMKKTSLY 182          
BLAST of Caltractin vs. Ensembl Fly
Match: CG17493 (gene:FBgn0040010 transcript:FBtr0332632)

HSP 1 Score: 102.064 bits (253), Expect = 4.011e-26
Identity = 54/142 (38.03%), Postives = 94/142 (66.20%), Query Frame = 3
Query:  321 DLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNLF 743
            D+K  FD+FD+   G++ + EL++++RALGF+    + + ++ ++  +    I FN FL  +     E +DT EE+++ F+ FD  DTG IS  NLK ++ EL   L+  +L++MI+EAD++ DG V++EEF++IM +T+L+
Sbjct:   42 DIKEAFDLFDNEGTGYIEVKELKVAIRALGFEPKKEEIKRMISDIDKDCSGRIAFNVFLQLMTIKMAE-KDTKEEILKAFRLFDDDDTGKISFRNLKRVARELGETLTDEELREMIDEADLDNDGEVNQEEFLRIMKKTSLY 182          
BLAST of Caltractin vs. Ensembl Fly
Match: CG17493 (gene:FBgn0040010 transcript:FBtr0332631)

HSP 1 Score: 102.064 bits (253), Expect = 4.011e-26
Identity = 54/142 (38.03%), Postives = 94/142 (66.20%), Query Frame = 3
Query:  321 DLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNLF 743
            D+K  FD+FD+   G++ + EL++++RALGF+    + + ++ ++  +    I FN FL  +     E +DT EE+++ F+ FD  DTG IS  NLK ++ EL   L+  +L++MI+EAD++ DG V++EEF++IM +T+L+
Sbjct:   42 DIKEAFDLFDNEGTGYIEVKELKVAIRALGFEPKKEEIKRMISDIDKDCSGRIAFNVFLQLMTIKMAE-KDTKEEILKAFRLFDDDDTGKISFRNLKRVARELGETLTDEELREMIDEADLDNDGEVNQEEFLRIMKKTSLY 182          
BLAST of Caltractin vs. Ensembl Fly
Match: CG31802 (gene:FBgn0051802 transcript:FBtr0081058)

HSP 1 Score: 101.679 bits (252), Expect = 5.710e-26
Identity = 63/165 (38.18%), Postives = 99/165 (60.00%), Query Frame = 3
Query:  276 ISSKALKELIPE---------EIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNL 740
            ISS+A K   P+         +  D+K  FD+FD+   GF+   ELR+++RALGF+      + +M E+  +    I FN FL  +     E +D+ ++MM+ F +FD   TG IS  NLK ++ EL   L+  +LQ+MI+EA+++GDG VS+EEF+ ++ +TNL
Sbjct:   22 ISSRAKKSKKPKLPTFDLSLSQKVDIKKAFDLFDTQCTGFIETKELRVAIRALGFEPKKEDIKRMMDEIDKDKTGRIAFNDFLYLMRLKMAE-KDSNQDMMKAFSFFDDDRTGGISFLNLKRVAKELGEQLTDEELQEMIDEANVSGDGEVSKEEFLNLIKKTNL 185          
BLAST of Caltractin vs. Ensembl Fly
Match: Cam (gene:FBgn0000253 transcript:FBtr0304964)

HSP 1 Score: 88.1965 bits (217), Expect = 2.403e-21
Identity = 52/145 (35.86%), Postives = 85/145 (58.62%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMT 728
            +L  E+I + K  F +FD + DG ++  EL   +R+LG   + A+ ++++ E+  +G   IDF  FL  +    K++ D+ EE+ + F+ FD    GFIS   L+ +   L   L+  ++ +MI EADI+GDG V+ EEFV +MT
Sbjct:    4 QLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDT-DSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVTMMT 147          
BLAST of Caltractin vs. Ensembl Zebrafish
Match: cetn4 (centrin 4 [Source:ZFIN;Acc:ZDB-GENE-030826-21])

HSP 1 Score: 109.768 bits (273), Expect = 3.750e-29
Identity = 56/150 (37.33%), Postives = 102/150 (68.00%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNLF 743
            EL  E+ +++K  FD+FD++  G + + EL++++RALGF+    + ++++ ++   G  +I F+ FL+ + +   E +D+ EE+++ F+ FD   TG IS  NLK ++ EL   L+  +LQ+MI+EAD +GDG ++E+EF++IM +TNL+
Sbjct:   23 ELTEEQKQEIKEAFDLFDTDGSGTIDVKELKVAMRALGFEPKKEEIKKMIADIDKEGSGVIGFSDFLSMMTQKMSE-KDSKEEILKAFRLFDDDCTGKISFKNLKRVAKELGENLTDEELQEMIDEADRDGDGEINEQEFLRIMKKTNLY 171          
BLAST of Caltractin vs. Ensembl Zebrafish
Match: cetn2 (centrin, EF-hand protein, 2 [Source:ZFIN;Acc:ZDB-GENE-091118-94])

HSP 1 Score: 107.457 bits (267), Expect = 3.523e-28
Identity = 53/150 (35.33%), Postives = 101/150 (67.33%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNLF 743
            EL  E+ ++++  F++FD++  G++ + EL++++RALGF+    + ++++ E+       I F  FL+ + +   E +D+ EE+++ F+ FD  +TG IS  NLK ++ EL   L+  +LQ+MI+EAD +GDG V+++EF++IM +T+L+
Sbjct:   24 ELTEEQKQEIREAFELFDTDGSGYIEVKELKVAMRALGFEPKKEEIKKMIAEVDKEATGKISFTDFLSVMTQKMAE-KDSKEEILKAFRLFDDDETGKISFRNLKRVAKELGENLTDEELQEMIDEADRDGDGEVNQQEFLRIMKKTSLY 172          
BLAST of Caltractin vs. Ensembl Zebrafish
Match: cetn3 (centrin 3 [Source:ZFIN;Acc:ZDB-GENE-050522-152])

HSP 1 Score: 90.1225 bits (222), Expect = 1.043e-21
Identity = 53/151 (35.10%), Postives = 88/151 (58.28%), Query Frame = 3
Query:  279 SSKALKELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMT 728
              K  +EL  E+  ++K  F++FD++ D  +   EL++++RALGF+       +++ +    G   I F  F   V +   E RD  EE+++ FK FD  +TG ISL NL+ ++ EL   +S   L+ MI+E D +GDG ++++EF+ IMT
Sbjct:   11 KRKKRRELTDEQKDEIKEAFELFDTDKDKEIDYHELKVAMRALGFEVKKVDVLKILKDYDREGTGKISFEDFREVVTDMILE-RDPKEEILKAFKLFDDDETGKISLRNLRRVARELGEDMSDEDLRAMIDEFDTDGDGEINQDEFISIMT 160          
BLAST of Caltractin vs. Ensembl Zebrafish
Match: cetn3 (centrin 3 [Source:ZFIN;Acc:ZDB-GENE-050522-152])

HSP 1 Score: 90.1225 bits (222), Expect = 1.071e-21
Identity = 53/149 (35.57%), Postives = 88/149 (59.06%), Query Frame = 3
Query:  285 KALKELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMT 728
            K  +EL  E+  ++K  F++FD++ D  +   EL++++RALGF+       +++ +    G   I F  F   V +   E RD  EE+++ FK FD  +TG ISL NL+ ++ EL   +S   L+ MI+E D +GDG ++++EF+ IMT
Sbjct:   17 KKRRELTDEQKDEIKEAFELFDTDKDKEIDYHELKVAMRALGFEVKKVDVLKILKDYDREGTGKISFEDFREVVTDMILE-RDPKEEILKAFKLFDDDETGKISLRNLRRVARELGEDMSDEDLRAMIDEFDTDGDGEINQDEFISIMT 164          
BLAST of Caltractin vs. Ensembl Zebrafish
Match: calm2a (calmodulin 2a (phosphorylase kinase, delta) [Source:ZFIN;Acc:ZDB-GENE-030804-3])

HSP 1 Score: 88.5817 bits (218), Expect = 2.786e-21
Identity = 51/145 (35.17%), Postives = 86/145 (59.31%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMT 728
            +L  E+I + K  F +FD + DG ++  EL   +R+LG   + A+ ++++ E+  +G   IDF  FL  +    K++ D+ EE+ + F+ FD    G+IS   L+ +   L   L+  ++ +MI EADI+GDG V+ EEFV++MT
Sbjct:    4 QLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDT-DSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMT 147          
BLAST of Caltractin vs. Ensembl Xenopus
Match: dtl (denticleless E3 ubiquitin protein ligase homolog [Source:Xenbase;Acc:XB-GENE-985623])

HSP 1 Score: 113.235 bits (282), Expect = 2.558e-30
Identity = 62/169 (36.69%), Postives = 109/169 (64.50%), Query Frame = 3
Query:  237 THPVGEKEVYNMVISSKALKELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNL 740
            T+P G        I +K   EL  E+ ++++  FD+FD++  G + + EL++++RALGF+    + ++++ +   +G  IIDF  FL+ + +   E +D+ EE+M+ F+ FD  +TG IS  NLK ++ EL   L+  +LQ+MI+EAD +GDG ++E+EF++IM +T+L
Sbjct:    6 TNPPGLGATQRRRIGAKP--ELTEEQKQEIREAFDLFDTDGSGTIDVKELKVAMRALGFEPKKEEMKKIISDTDKDGSGIIDFEDFLSLMTQKMSE-KDSKEEIMKAFRLFDDDNTGKISFKNLKRVAKELGENLTDEELQEMIDEADRDGDGEINEQEFLRIMRKTSL 171          
BLAST of Caltractin vs. Ensembl Xenopus
Match: dtl (denticleless E3 ubiquitin protein ligase homolog [Source:Xenbase;Acc:XB-GENE-985623])

HSP 1 Score: 111.694 bits (278), Expect = 1.044e-29
Identity = 58/152 (38.16%), Postives = 103/152 (67.76%), Query Frame = 3
Query:  288 ALKELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNL 740
            A  EL  E+ ++++  FD+FD++  G + + EL++++RALGF+    + ++++ +   +G  IIDF  FL+ + +   E +D+ EE+M+ F+ FD  +TG IS  NLK ++ EL   L+  +LQ+MI+EAD +GDG ++E+EF++IM +T+L
Sbjct:   20 AKPELTEEQKQEIREAFDLFDTDGSGTIDVKELKVAMRALGFEPKKEEMKKIISDTDKDGSGIIDFEDFLSLMTQKMSE-KDSKEEIMKAFRLFDDDNTGKISFKNLKRVAKELGENLTDEELQEMIDEADRDGDGEINEQEFLRIMRKTSL 170          
BLAST of Caltractin vs. Ensembl Xenopus
Match: cetn1 (centrin 1 [Source:NCBI gene;Acc:549903])

HSP 1 Score: 109.383 bits (272), Expect = 4.605e-29
Identity = 55/150 (36.67%), Postives = 101/150 (67.33%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNLF 743
            EL  E+ ++++  FD+FD++  G + + EL++++RALGF+    + ++++ ++   G   I F  F++ + +   E +D+ EE+M+ F+ FD  +TG IS  NLK ++ EL   L+  +LQ+MI+EAD +GDG V+E+EF++IM +T+L+
Sbjct:    9 ELTEEQKQEIREAFDLFDTDGAGTIDVKELKVAMRALGFEPKKEEIKKMIADIDKEGTGKISFGDFMSAMTQKMAE-KDSKEEIMKAFRLFDDDETGKISFKNLKRVAKELGENLTDEELQEMIDEADRDGDGEVNEQEFLRIMKKTSLY 157          
BLAST of Caltractin vs. Ensembl Xenopus
Match: cetn1 (centrin 1 [Source:NCBI gene;Acc:549903])

HSP 1 Score: 109.768 bits (273), Expect = 5.608e-29
Identity = 55/150 (36.67%), Postives = 101/150 (67.33%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNLF 743
            EL  E+ ++++  FD+FD++  G + + EL++++RALGF+    + ++++ ++   G   I F  F++ + +   E +D+ EE+M+ F+ FD  +TG IS  NLK ++ EL   L+  +LQ+MI+EAD +GDG V+E+EF++IM +T+L+
Sbjct:   24 ELTEEQKQEIREAFDLFDTDGAGTIDVKELKVAMRALGFEPKKEEIKKMIADIDKEGTGKISFGDFMSAMTQKMAE-KDSKEEIMKAFRLFDDDETGKISFKNLKRVAKELGENLTDEELQEMIDEADRDGDGEVNEQEFLRIMKKTSLY 172          
BLAST of Caltractin vs. Ensembl Xenopus
Match: rab38 (RAB38, member RAS oncogene family [Source:Xenbase;Acc:XB-GENE-480756])

HSP 1 Score: 91.6633 bits (226), Expect = 4.365e-22
Identity = 54/154 (35.06%), Postives = 92/154 (59.74%), Query Frame = 3
Query:  279 SSKALKELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELME----LSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMT 728
              K  +EL  E+ +++K  F++FD++ D  +   EL++++RALGF    A   ++++     +T  I   DFN  +  +I +    RD  EE+++ FK FD  D+G ISL NL+ ++ EL   ++  +L+ MI E D +GDG +++EEF+ IMT
Sbjct:   15 KKKKRRELTEEQKQEIKDAFELFDTDKDKAIDYHELKVAMRALGFDVKKADVLKILKDYDGETTGKITFDDFNEVVTDLILD----RDPQEEILKAFKLFDDDDSGKISLRNLRRVARELGENMTDEELRAMIEEFDKDGDGEINQEEFLSIMT 164          
BLAST of Caltractin vs. Ensembl Mouse
Match: Cetn1 (centrin 1 [Source:MGI Symbol;Acc:MGI:1347086])

HSP 1 Score: 114.39 bits (285), Expect = 8.278e-31
Identity = 60/150 (40.00%), Postives = 100/150 (66.67%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNLF 743
            EL  ++ ++++  FD+FDS+  G + + EL++++RALGF+    + ++++ E+       I FN FL  + +   E +DT EE+++ F+ FD  +TG IS  NLK ++ EL   L+  +LQ+MI+EAD +GDG V+EEEF+KIM +TNL+
Sbjct:   24 ELTEDQKQEVREAFDLFDSDGSGTIDVKELKVAMRALGFEPRKEEMKKMISEVDKEATGKISFNDFLAVMTQKMAE-KDTKEEILKAFRLFDDDETGKISFKNLKRVANELGESLTDEELQEMIDEADRDGDGEVNEEEFLKIMKKTNLY 172          
BLAST of Caltractin vs. Ensembl Mouse
Match: Cetn1 (centrin 1 [Source:MGI Symbol;Acc:MGI:1347086])

HSP 1 Score: 114.39 bits (285), Expect = 8.278e-31
Identity = 60/150 (40.00%), Postives = 100/150 (66.67%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNLF 743
            EL  ++ ++++  FD+FDS+  G + + EL++++RALGF+    + ++++ E+       I FN FL  + +   E +DT EE+++ F+ FD  +TG IS  NLK ++ EL   L+  +LQ+MI+EAD +GDG V+EEEF+KIM +TNL+
Sbjct:   24 ELTEDQKQEVREAFDLFDSDGSGTIDVKELKVAMRALGFEPRKEEMKKMISEVDKEATGKISFNDFLAVMTQKMAE-KDTKEEILKAFRLFDDDETGKISFKNLKRVANELGESLTDEELQEMIDEADRDGDGEVNEEEFLKIMKKTNLY 172          
BLAST of Caltractin vs. Ensembl Mouse
Match: Cetn1 (centrin 1 [Source:MGI Symbol;Acc:MGI:1347086])

HSP 1 Score: 114.39 bits (285), Expect = 8.278e-31
Identity = 60/150 (40.00%), Postives = 100/150 (66.67%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNLF 743
            EL  ++ ++++  FD+FDS+  G + + EL++++RALGF+    + ++++ E+       I FN FL  + +   E +DT EE+++ F+ FD  +TG IS  NLK ++ EL   L+  +LQ+MI+EAD +GDG V+EEEF+KIM +TNL+
Sbjct:   24 ELTEDQKQEVREAFDLFDSDGSGTIDVKELKVAMRALGFEPRKEEMKKMISEVDKEATGKISFNDFLAVMTQKMAE-KDTKEEILKAFRLFDDDETGKISFKNLKRVANELGESLTDEELQEMIDEADRDGDGEVNEEEFLKIMKKTNLY 172          
BLAST of Caltractin vs. Ensembl Mouse
Match: Cetn2 (centrin 2 [Source:MGI Symbol;Acc:MGI:1347085])

HSP 1 Score: 110.538 bits (275), Expect = 1.651e-29
Identity = 57/150 (38.00%), Postives = 102/150 (68.00%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNLF 743
            EL  ++ ++++  FD+FD++  G + I EL++++RALGF+    + ++++ E+   G   ++F+ FL  + +   E +DT EE+++ FK FD  +TG IS  NLK ++ EL   L+  +LQ+MI+EAD +GDG V+E+EF++IM +T+L+
Sbjct:   15 ELTEDQKQEIREAFDLFDADGTGTIDIKELKVAMRALGFEPKKEEIKKMISEIDKEGTGKMNFSDFLTVMTQKMSE-KDTKEEILKAFKLFDDDETGKISFKNLKRVAKELGENLTDEELQEMIDEADRDGDGEVNEQEFLRIMKKTSLY 163          
BLAST of Caltractin vs. Ensembl Mouse
Match: Cetn2 (centrin 2 [Source:MGI Symbol;Acc:MGI:1347085])

HSP 1 Score: 110.538 bits (275), Expect = 1.651e-29
Identity = 57/150 (38.00%), Postives = 102/150 (68.00%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNLF 743
            EL  ++ ++++  FD+FD++  G + I EL++++RALGF+    + ++++ E+   G   ++F+ FL  + +   E +DT EE+++ FK FD  +TG IS  NLK ++ EL   L+  +LQ+MI+EAD +GDG V+E+EF++IM +T+L+
Sbjct:   15 ELTEDQKQEIREAFDLFDADGTGTIDIKELKVAMRALGFEPKKEEIKKMISEIDKEGTGKMNFSDFLTVMTQKMSE-KDTKEEILKAFKLFDDDETGKISFKNLKRVAKELGENLTDEELQEMIDEADRDGDGEVNEQEFLRIMKKTSLY 163          
BLAST of Caltractin vs. UniProt/SwissProt
Match: sp|P41209|CETN1_MOUSE (Centrin-1 OS=Mus musculus OX=10090 GN=Cetn1 PE=1 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 5.791e-30
Identity = 60/150 (40.00%), Postives = 100/150 (66.67%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNLF 743
            EL  ++ ++++  FD+FDS+  G + + EL++++RALGF+    + ++++ E+       I FN FL  + +   E +DT EE+++ F+ FD  +TG IS  NLK ++ EL   L+  +LQ+MI+EAD +GDG V+EEEF+KIM +TNL+
Sbjct:   24 ELTEDQKQEVREAFDLFDSDGSGTIDVKELKVAMRALGFEPRKEEMKKMISEVDKEATGKISFNDFLAVMTQKMAE-KDTKEEILKAFRLFDDDETGKISFKNLKRVANELGESLTDEELQEMIDEADRDGDGEVNEEEFLKIMKKTNLY 172          
BLAST of Caltractin vs. UniProt/SwissProt
Match: sp|P41208|CETN2_HUMAN (Centrin-2 OS=Homo sapiens OX=9606 GN=CETN2 PE=1 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 9.542e-30
Identity = 62/165 (37.58%), Postives = 107/165 (64.85%), Query Frame = 3
Query:  267 NMVISSKALK-----ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNLF 743
            NM  SS+  +     EL  E+ ++++  FD+FD++  G + + EL++++RALGF+    + ++++ E+   G   ++F  FL  + +   E +DT EE+++ FK FD  +TG IS  NLK ++ EL   L+  +LQ+MI+EAD +GDG VSE+EF++IM +T+L+
Sbjct:    9 NMASSSQRKRMSPKPELTEEQKQEIREAFDLFDADGTGTIDVKELKVAMRALGFEPKKEEIKKMISEIDKEGTGKMNFGDFLTVMTQKMSE-KDTKEEILKAFKLFDDDETGKISFKNLKRVAKELGENLTDEELQEMIDEADRDGDGEVSEQEFLRIMKKTSLY 172          
BLAST of Caltractin vs. UniProt/SwissProt
Match: sp|Q32LE3|CETN1_BOVIN (Centrin-1 OS=Bos taurus OX=9913 GN=CETN1 PE=2 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 1.218e-29
Identity = 58/150 (38.67%), Postives = 100/150 (66.67%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNLF 743
            EL  E+ ++++  FD+FD++  G + + EL++++RALGF+    + + ++ ++   G   I FN FL  + +   E +DT EE+++ F+ FD  +TG IS  NLK ++ EL   L+  +LQ+MI+EAD +GDG V+E+EF++IM +TNL+
Sbjct:   24 ELTEEQKQEVREAFDLFDADGSGTIDVKELKVAMRALGFEPRKEEMKRMIADVDKEGTGKISFNDFLAVMTQKMAE-KDTKEEILKAFRLFDDDETGKISFKNLKRVAKELGENLTDEELQEMIDEADRDGDGEVNEDEFLRIMKKTNLY 172          
BLAST of Caltractin vs. UniProt/SwissProt
Match: sp|P43646|CATR_TETST (Caltractin (Fragment) OS=Tetraselmis striata OX=3165 PE=2 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 2.545e-29
Identity = 59/149 (39.60%), Postives = 98/149 (65.77%), Query Frame = 3
Query:  300 LIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNLF 743
            L  E+ +D++  FD+FD++  G +   EL++++RALGF+    + ++++ ++  +G   IDF  FL  +     E RD+ EE+M+ F+ FD  +TG IS  NLK ++ EL   ++  +LQ+MI+EAD +GDG V+EEEF +IM +T+LF
Sbjct:    1 LTEEQKQDIREAFDLFDTDGSGTIDAKELKVAMRALGFEPKKEEIKKMIADIDKDGSGTIDFEEFLQMMTAKMGE-RDSREEIMKAFRLFDDDETGKISFKNLKRVAKELGENMTDEELQEMIDEADRDGDGEVNEEEFFRIMKKTSLF 148          
BLAST of Caltractin vs. UniProt/SwissProt
Match: sp|Q2TBN3|CETN2_BOVIN (Centrin-2 OS=Bos taurus OX=9913 GN=CETN2 PE=2 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 3.306e-29
Identity = 58/150 (38.67%), Postives = 102/150 (68.00%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNLF 743
            EL  E+ ++++  FD+FD++  G + + EL++++RALGF+    + ++++ E+   G   ++F+ FL  + +   E +DT EE+++ FK FD  +TG IS  NLK ++ EL   LS  +LQ+MI+EAD +GDG V+E+EF++IM +T+L+
Sbjct:   24 ELTEEQKQEIREAFDLFDADGTGTIDVKELKVAMRALGFEPKKEEIKKMISEIDKEGTGKMNFSDFLTVMTQKMSE-KDTKEEILKAFKLFDDDETGKISFKNLKRVAKELGENLSDEELQEMIDEADRDGDGEVNEQEFLRIMKKTSLY 172          
BLAST of Caltractin vs. TrEMBL
Match: A0A267E013 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig015188g2 PE=4 SV=1)

HSP 1 Score: 188.348 bits (477), Expect = 2.059e-55
Identity = 98/195 (50.26%), Postives = 131/195 (67.18%), Query Frame = 3
Query:  159 SEGYLIKDPKPIYVPLPGENLLKVLTTHPVGEKEVYNMVISSKALKELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELMELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNLF 743
            S   L+  P P  +PL GENLL VL TH V EKE++NM I  +AL EL P+EI+DLK+VFDVFDS+ DG LS+ E+R++LRALGFK    Q  ++      G+   DFN FLN VI+ Q + RD  +E++ GFK+FDL  TG ++L NLK L  +  V +   +L++MI EAD NGD  V  EEFVK+M QT+++
Sbjct:   37 SGSVLMPKPLPPNIPLLGENLLHVLKTHEVEEKELHNMKIGVRALDELTPQEIRDLKLVFDVFDSDLDGNLSLAEVRLALRALGFKTDMDQVHKMATELNKGLVATDFNAFLNFVIDRQGDVRDLHDEIVLGFKHFDLDGTGKVTLANLKKLCRDYKVRIPDSELEEMIQEADTNGDNAVDVEEFVKVMMQTHMY 231          
BLAST of Caltractin vs. TrEMBL
Match: A0A1I8G6T3 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 186.808 bits (473), Expect = 2.116e-55
Identity = 96/187 (51.34%), Postives = 128/187 (68.45%), Query Frame = 3
Query:  183 PKPIYVPLPGENLLKVLTTHPVGEKEVYNMVISSKALKELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELMELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNLF 743
            P P  +PL GENLL VL TH V EKE++NM I  +AL EL P+EI+DLK+VFDVFDS+ DG LS+ E+R++LRALGFK    Q  ++      G+   DFN FLN VI+ Q + RD  +E++ GFK+FDL  TG ++L NLK L  +  V +   +L++MI EAD NGD  V  EEFVK+M QT+++
Sbjct:    4 PLPPNIPLLGENLLHVLKTHEVEEKELHNMKIGVRALDELTPQEIRDLKLVFDVFDSDLDGNLSLAEVRLALRALGFKTDMDQVHKMATELNKGLVATDFNAFLNFVIDRQGDVRDLHDEIVLGFKHFDLDGTGKVTLANLKKLCRDYKVRIPDSELEEMIQEADTNGDNAVDVEEFVKVMMQTHMY 190          
BLAST of Caltractin vs. TrEMBL
Match: A0A267FW94 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig015188g3 PE=4 SV=1)

HSP 1 Score: 187.963 bits (476), Expect = 2.997e-55
Identity = 98/195 (50.26%), Postives = 131/195 (67.18%), Query Frame = 3
Query:  159 SEGYLIKDPKPIYVPLPGENLLKVLTTHPVGEKEVYNMVISSKALKELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELMELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNLF 743
            S   L+  P P  +PL GENLL VL TH V EKE++NM I  +AL EL P+EI+DLK+VFDVFDS+ DG LS+ E+R++LRALGFK    Q  ++      G+   DFN FLN VI+ Q + RD  +E++ GFK+FDL  TG ++L NLK L  +  V +   +L++MI EAD NGD  V  EEFVK+M QT+++
Sbjct:   44 SGSVLMPKPLPPNIPLLGENLLHVLKTHEVEEKELHNMKIGVRALDELTPQEIRDLKLVFDVFDSDLDGNLSLAEVRLALRALGFKTDMDQVHKMATELNKGLVATDFNAFLNFVIDRQGDVRDLHDEIVLGFKHFDLDGTGKVTLANLKKLCRDYKVRIPDSELEEMIQEADTNGDNAVDVEEFVKVMMQTHMY 238          
BLAST of Caltractin vs. TrEMBL
Match: A0A1I8GNX6 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 187.578 bits (475), Expect = 3.618e-55
Identity = 98/195 (50.26%), Postives = 131/195 (67.18%), Query Frame = 3
Query:  159 SEGYLIKDPKPIYVPLPGENLLKVLTTHPVGEKEVYNMVISSKALKELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELMELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNLF 743
            S   L+  P P  +PL GENLL VL TH V EKE++NM I  +AL EL P+EI+DLK+VFDVFDS+ DG LS+ E+R++LRALGFK    Q  ++      G+   DFN FLN VI+ Q + RD  +E++ GFK+FDL  TG ++L NLK L  +  V +   +L++MI EAD NGD  V  EEFVK+M QT+++
Sbjct:   42 SGSVLMPKPLPPNIPLLGENLLHVLKTHEVEEKELHNMKIGVRALDELTPQEIRDLKLVFDVFDSDLDGNLSLAEVRLALRALGFKTDMDQVHKMATELNKGLVATDFNAFLNFVIDRQGDVRDLHDEIVLGFKHFDLDGTGKVTLANLKKLCRDYKVRIPDSELEEMIQEADTNGDNAVDVEEFVKVMMQTHMY 236          
BLAST of Caltractin vs. TrEMBL
Match: V4B6X7 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_196719 PE=4 SV=1)

HSP 1 Score: 185.652 bits (470), Expect = 1.542e-54
Identity = 92/189 (48.68%), Postives = 134/189 (70.90%), Query Frame = 3
Query:  183 PKPIYVP-LPGENLLKVLTTHPVGEKEVYNMVISSKALKELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELMELSTN-GIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNLF 743
            P+ +  P LP E L+ +LT++PV EKEV+   +S++ L EL P+EI+DL++VFD+FD NNDG +   ELR SL+ALGFK S A A +L+  S+N G  +I+F  FL  VI+ Q ++RD ++E+++GF+ FDL  TG ISLDNL  +  E  +  +   L++MI EAD+NGDG++ + EF++IM QTNLF
Sbjct:   33 PEAVAQPILPREKLVNLLTSNPVDEKEVHGFKVSTRVLNELTPQEIRDLRVVFDLFDINNDGLIGAAELRRSLKALGFKVSKADAHKLIADSSNKGSSLINFTEFLETVIDRQGDTRDVYDEIIKGFQMFDLEKTGSISLDNLIKICQETGLKFTKKDLEEMIVEADVNGDGLIDQNEFIRIMLQTNLF 221          
BLAST of Caltractin vs. Ensembl Cavefish
Match: cetn4 (caltractin [Source:NCBI gene;Acc:103037928])

HSP 1 Score: 110.153 bits (274), Expect = 2.586e-29
Identity = 55/150 (36.67%), Postives = 102/150 (68.00%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNLF 743
            +L  E+ ++++  FD+FD++  G + + EL++++RALGF+    + ++++ ++   G   I+FN FL+ + +   E +D+ EE+++ F+ FD   TG IS  NLK ++ EL   L+  +LQ+MI+EAD +GDG ++E+EF++IM +TNL+
Sbjct:   24 DLTEEQKQEIREAFDLFDTDGSGTIDVKELKVAMRALGFEPKKEEIKKMIADIDKEGSGTINFNDFLSMMTQKMSE-KDSKEEILKAFRLFDDDGTGKISFKNLKRVAKELGENLTDEELQEMIDEADRDGDGEINEQEFLRIMKKTNLY 172          
BLAST of Caltractin vs. Ensembl Cavefish
Match: cetn4 (caltractin [Source:NCBI gene;Acc:103037928])

HSP 1 Score: 109.768 bits (273), Expect = 2.951e-29
Identity = 55/150 (36.67%), Postives = 102/150 (68.00%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNLF 743
            +L  E+ ++++  FD+FD++  G + + EL++++RALGF+    + ++++ ++   G   I+FN FL+ + +   E +D+ EE+++ F+ FD   TG IS  NLK ++ EL   L+  +LQ+MI+EAD +GDG ++E+EF++IM +TNL+
Sbjct:   23 DLTEEQKQEIREAFDLFDTDGSGTIDVKELKVAMRALGFEPKKEEIKKMIADIDKEGSGTINFNDFLSMMTQKMSE-KDSKEEILKAFRLFDDDGTGKISFKNLKRVAKELGENLTDEELQEMIDEADRDGDGEINEQEFLRIMKKTNLY 171          
BLAST of Caltractin vs. Ensembl Cavefish
Match: cetn2 (centrin-1 [Source:NCBI gene;Acc:103028142])

HSP 1 Score: 105.145 bits (261), Expect = 1.879e-27
Identity = 54/150 (36.00%), Postives = 100/150 (66.67%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNLF 743
            EL  E  ++++  F++FD++  G + + EL++++RALGF+    + ++++ ++  +G   I FN FL  + +   E +D+ EE+++ F+ FD  +TG IS  NLK ++ EL   L+  +LQ+MI+EAD +GDG V+++EF++IM +T L+
Sbjct:   24 ELTEEMKQEIREAFELFDTDGSGHIEVKELKVAMRALGFEPKKEEIKKMIADVDKDGTGKISFNDFLTIMTQKMAE-KDSKEEILKAFRLFDDDETGKISFRNLKRVAKELGENLTDEELQEMIDEADRDGDGEVNQQEFLRIMKKTCLY 172          
BLAST of Caltractin vs. Ensembl Cavefish
Match: cetn3 (centrin 3 [Source:NCBI gene;Acc:103024517])

HSP 1 Score: 92.8189 bits (229), Expect = 8.854e-23
Identity = 54/150 (36.00%), Postives = 89/150 (59.33%), Query Frame = 3
Query:  282 SKALKELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMT 728
             K  +EL  E+  ++K  F++FD++ D  +   EL++++RALGF+       +++ +    G   I F  F   V +   E RD  EE+++ FK FD  ++G ISL NL+ ++ EL   +S  +L+ MI+E D +GDG +++EEFV IMT
Sbjct:   16 RKKRRELTEEQKDEIKEAFELFDTDKDKEIDYHELKVAMRALGFEVKKVDVLKILKDYDREGTGKITFEDFREVVTDRILE-RDPKEEILKAFKLFDDDESGKISLRNLRRVARELGEDMSDEELRAMIDEFDTDGDGEINQEEFVSIMT 164          
BLAST of Caltractin vs. Ensembl Cavefish
Match: calm3a (calmodulin [Source:NCBI gene;Acc:103037741])

HSP 1 Score: 88.1965 bits (217), Expect = 2.676e-21
Identity = 51/145 (35.17%), Postives = 86/145 (59.31%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMT 728
            +L  E+I + K  F +FD + DG ++  EL   +R+LG   + A+ ++++ E+  +G   IDF  FL  +    K++ D+ EE+ + F+ FD    G+IS   L+ +   L   L+  ++ +MI EADI+GDG V+ EEFV++MT
Sbjct:    4 QLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDT-DSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMT 147          
BLAST of Caltractin vs. Ensembl Sea Lamprey
Match: CETN1 (centrin 1 [Source:HGNC Symbol;Acc:HGNC:1866])

HSP 1 Score: 110.923 bits (276), Expect = 3.846e-30
Identity = 56/150 (37.33%), Postives = 102/150 (68.00%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNLF 743
            EL  E+ +++K  FD+FD++  G + + EL++++RALGF+    + ++++ +   +G   I+FN FL  + +   E +D+ EE+++ F+ FD  +TG IS  NLK ++ EL   L+  +LQ+MI+EAD +GDG ++EEEF+++M +T+L+
Sbjct:   35 ELSEEQKQEIKEAFDLFDADGSGTIDVKELKVAMRALGFEPKKEEIKKMIADFDKDGSGKINFNDFLAMMTQKMSE-KDSREEILKAFRLFDDDETGKISFKNLKRVAKELGETLTDEELQEMIDEADRDGDGEINEEEFLRVMKKTSLY 183          
BLAST of Caltractin vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006008.1 (pep scaffold:Pmarinus_7.0:GL476713:142259:146509:-1 gene:ENSPMAG00000005430.1 transcript:ENSPMAT00000006008.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 89.7373 bits (221), Expect = 1.704e-22
Identity = 51/145 (35.17%), Postives = 87/145 (60.00%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMT 728
            +L  E+I + K  F +FD + DG +++ EL   +R+LG   + A+ ++++ E+  +G   IDF  FL  +    K++ D+ EE+ + F+ FD    G+IS   L+ +   L   L+  ++ +MI EADI+GDG V+ EEFV++MT
Sbjct:    4 QLTEEQIAEFKEAFSLFDKDGDGTITVKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDT-DSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMT 147          
BLAST of Caltractin vs. Ensembl Sea Lamprey
Match: CALML3 (calmodulin like 3 [Source:HGNC Symbol;Acc:HGNC:1452])

HSP 1 Score: 88.5817 bits (218), Expect = 4.790e-22
Identity = 51/145 (35.17%), Postives = 86/145 (59.31%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMT 728
            +L  E+I + K  F +FD + DG ++  EL   +R+LG   + A+ ++++ E+  +G   IDF  FL  +    K++ D+ EE+ + F+ FD    G+IS   L+ +   L   L+  ++ +MI EADI+GDG V+ EEFV++MT
Sbjct:    4 QLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDT-DSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMT 147          
BLAST of Caltractin vs. Ensembl Sea Lamprey
Match: CALM3 (calmodulin 3 [Source:HGNC Symbol;Acc:HGNC:1449])

HSP 1 Score: 88.1965 bits (217), Expect = 5.429e-22
Identity = 51/145 (35.17%), Postives = 86/145 (59.31%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMT 728
            +L  E+I + K  F +FD + DG ++  EL   +R+LG   + A+ ++++ E+  +G   IDF  FL  +    K++ D+ EE+ + F+ FD    G+IS   L+ +   L   L+  ++ +MI EADI+GDG V+ EEFV++MT
Sbjct:    4 QLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDT-DSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMT 147          
BLAST of Caltractin vs. Ensembl Sea Lamprey
Match: cetn3 (centrin 3 [Source:ZFIN;Acc:ZDB-GENE-050522-152])

HSP 1 Score: 88.9669 bits (219), Expect = 5.571e-22
Identity = 47/145 (32.41%), Postives = 90/145 (62.07%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMT 728
            E+  E+ ++++  F++FD++ DG +   EL++++RALGF+ S A   ++M +        I ++ F   + E   E RD  +E+++ F+ FD  ++G I + +L+ ++ EL   +S  +L+ MI+E D +GDG +++EEF+ IMT
Sbjct:   22 EMTEEQKQEIQEAFELFDTDKDGAIDYHELKVAIRALGFEVSKADVIKIMKDYDREDTGKISYDDFCEVMTERVLE-RDPLDEVLKAFRLFDEGESGRIGVRDLRRVARELGETVSDEELRAMIDEFDTDGDGEINQEEFIAIMT 165          
BLAST of Caltractin vs. Ensembl Yeast
Match: CDC31 (Calcium-binding component of the spindle pole body (SPB) half-bridge; required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation [Source:SGD;Acc:S000005783])

HSP 1 Score: 91.2781 bits (225), Expect = 4.004e-23
Identity = 51/147 (34.69%), Postives = 90/147 (61.22%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQT 734
            EL+ E+ +++   F +FD NNDGFL   EL+++++ALGF+    +  +L+ E  + G  ++ ++ F   V+  +   RD  +E+ + F+ FD   TG IS+ NL+ ++ EL   L+  +L+ MI E D++GDG ++E EF+ I T +
Sbjct:   16 ELLEEQKQEIYEAFSLFDMNNDGFLDYHELKVAMKALGFELPKREILDLIDEYDSEGRHLMKYDDFY-IVMGEKILKRDPLDEIKRAFQLFDDDHTGKISIKNLRRVAKELGETLTDEELRAMIEEFDLDGDGEINENEFIAICTDS 161          
BLAST of Caltractin vs. Ensembl Yeast
Match: CMD1 (Calmodulin; Ca2+ binding protein that regulates Ca2+ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca2+ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin; binds to the Hog1p MAPK in response to hyperosmotic stress; potentiates membrane tubulation and constriction mediated by the Rvs161p-Rvs167p complex; human CALM1 or CALM2 functionally complement repression induced inviability [Source:SGD;Acc:S000000313])

HSP 1 Score: 68.1662 bits (165), Expect = 8.190e-15
Identity = 44/145 (30.34%), Postives = 82/145 (56.55%), Query Frame = 3
Query:  300 LIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQ 731
            L  E+I + K  F +FD +N+G +S  EL   +R+LG   S A+  +LM E+  +G   I+F+ FL  ++  Q +S D+ +E+++ FK FD +  G IS   LK +   +   L+  ++  M+ E   +G G ++ ++F  ++++
Sbjct:    5 LTEEQIAEFKEAFALFDKDNNGSISSSELATVMRSLGLSPSEAEVNDLMNEIDVDGNHQIEFSEFL-ALMSRQLKSNDSEQELLEAFKVFDKNGDGLISAAELKHVLTSIGEKLTDAEVDDMLREVS-DGSGEINIQQFAALLSK 147          
BLAST of Caltractin vs. Ensembl Nematostella
Match: EDO41859 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S2Y1])

HSP 1 Score: 125.176 bits (313), Expect = 1.002e-35
Identity = 64/157 (40.76%), Postives = 102/157 (64.97%), Query Frame = 3
Query:  276 ISSKALKELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQ-AEELMELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNLF 743
            + ++A + L P+EI+DLK+VFD FD++  G +   ELR ++R LGFK S    A  + +L  +    I+F+ FL+ +I  Q + RD  +E++QGFK FD   +G I+L+NLK +S    V L+  +L++MI EAD +GD  + ++EF+ +M +TNLF
Sbjct:    5 VGARAYQHLTPQEIRDLKLVFDTFDTDKSGSIDGRELRKAMRTLGFKISKEGIAGMIADLDADQSGRIEFDEFLDFIISKQSDGRDVHDEIVQGFKMFDTDQSGRITLENLKQVSRMCGVKLNETELKEMILEADKDGDSSIDQDEFINMMLKTNLF 161          
BLAST of Caltractin vs. Ensembl Nematostella
Match: EDO40775 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S690])

HSP 1 Score: 110.153 bits (274), Expect = 6.968e-30
Identity = 57/150 (38.00%), Postives = 100/150 (66.67%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNLF 743
            EL  E+ ++++  FD+FD++  G +   EL++++RALGF+    + ++++ ++  +G   IDFN FL  +     E +D+ EE+++ FK FD  +TG IS  NLK ++ EL   L+  +LQ+MI+EAD +GDG ++E EF++IM +T+L+
Sbjct:   14 ELTEEQKQEIREAFDLFDTDGSGTIDAKELKVAMRALGFEPKKEEIKKMISDIDKDGSGTIDFNEFLQMMTAKMSE-KDSKEEILKAFKLFDDDETGKISFKNLKRVAKELGENLTDEELQEMIDEADRDGDGEINEAEFLRIMKKTSLY 162          
BLAST of Caltractin vs. Ensembl Nematostella
Match: EDO46518 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RPI8])

HSP 1 Score: 90.5077 bits (223), Expect = 1.430e-22
Identity = 53/145 (36.55%), Postives = 86/145 (59.31%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMT 728
            +L  E+I + K  F +FD + DG ++  EL   +R+LG   + A+ ++++ E+  +G   IDF  FL  +    K++ D+ EE+ + F+ FD    GFIS   L+ +   L   L+  ++ +MI EADI+GDG V+ EEFVK+MT
Sbjct:    4 QLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDT-DSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVKMMT 147          
BLAST of Caltractin vs. Ensembl Nematostella
Match: EDO44944 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RU39])

HSP 1 Score: 89.3521 bits (220), Expect = 6.410e-22
Identity = 55/154 (35.71%), Postives = 91/154 (59.09%), Query Frame = 3
Query:  279 SSKALKELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELME----LSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMT 728
            S    +EL  E+ +++K  F++FD++ D  +   EL++++RALGF    A   ++M+     ST  I   DFN     V+ +    RD  EE+ + F+ FD  D+G ISL NL+ ++ EL   ++  +L+ MI+E D +GDG ++E+EF+ IMT
Sbjct:   16 SKPKRRELGEEQRQEIKEAFNLFDTDKDQAIDYHELKVAMRALGFDVKKADVLKIMKDYDRESTGKISFDDFN----EVMTDWMLDRDPQEEVFKAFRLFDDDDSGKISLRNLRRVARELGENMTDEELRAMIDEFDKDGDGEINEDEFLAIMT 165          
BLAST of Caltractin vs. Ensembl Nematostella
Match: EDO46606 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RPN8])

HSP 1 Score: 82.8037 bits (203), Expect = 7.973e-20
Identity = 49/139 (35.25%), Postives = 81/139 (58.27%), Query Frame = 3
Query:  315 IKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMT 728
            + + K  F +FD + DG ++  EL   +R+LG   + A+ ++++ E+  +G   IDF  FL  +    K + D+ EE+ + F+ FD    GFIS   L+ +   L   L+  ++ +MI EADI+GDG V+ +EFVK+MT
Sbjct:    1 MTEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKNT-DSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYDEFVKMMT 138          
BLAST of Caltractin vs. Ensembl Medaka
Match: cetn2 (caltractin [Source:NCBI gene;Acc:101170769])

HSP 1 Score: 106.301 bits (264), Expect = 7.727e-28
Identity = 54/150 (36.00%), Postives = 100/150 (66.67%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNLF 743
            EL  E+ ++++  F++FD++  GF+ + +L++++RALGF+    + ++++ E+  +    I F  FL  + +   E +D+ EE+++ F+ FD  +TG IS  NLK ++ EL   L+  +LQ+MI+EAD +GDG V++EEF++IM +T L+
Sbjct:   27 ELTEEQKQEIREAFELFDTDGSGFIDVKDLKVAMRALGFEPKKEEIKKMIGEVDKDATGKISFADFLTVMTQKMAE-KDSKEEILKAFRLFDDDETGKISFRNLKRVAKELGENLTDEELQEMIDEADRDGDGEVNQEEFLRIMKKTCLY 175          
BLAST of Caltractin vs. Ensembl Medaka
Match: cetn2 (caltractin [Source:NCBI gene;Acc:101170769])

HSP 1 Score: 106.301 bits (264), Expect = 7.727e-28
Identity = 54/150 (36.00%), Postives = 100/150 (66.67%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNLF 743
            EL  E+ ++++  F++FD++  GF+ + +L++++RALGF+    + ++++ E+  +    I F  FL  + +   E +D+ EE+++ F+ FD  +TG IS  NLK ++ EL   L+  +LQ+MI+EAD +GDG V++EEF++IM +T L+
Sbjct:   27 ELTEEQKQEIREAFELFDTDGSGFIDVKDLKVAMRALGFEPKKEEIKKMIGEVDKDATGKISFADFLTVMTQKMAE-KDSKEEILKAFRLFDDDETGKISFRNLKRVAKELGENLTDEELQEMIDEADRDGDGEVNQEEFLRIMKKTCLY 175          
BLAST of Caltractin vs. Ensembl Medaka
Match: calm2a (calmodulin 2 [Source:NCBI gene;Acc:100049420])

HSP 1 Score: 88.1965 bits (217), Expect = 2.660e-21
Identity = 51/145 (35.17%), Postives = 86/145 (59.31%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMT 728
            +L  E+I + K  F +FD + DG ++  EL   +R+LG   + A+ ++++ E+  +G   IDF  FL  +    K++ D+ EE+ + F+ FD    G+IS   L+ +   L   L+  ++ +MI EADI+GDG V+ EEFV++MT
Sbjct:    4 QLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDT-DSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMT 147          
BLAST of Caltractin vs. Ensembl Medaka
Match: ENSORLT00000008599.2 (calmodulin 3 [Source:NCBI gene;Acc:100049421])

HSP 1 Score: 88.1965 bits (217), Expect = 2.660e-21
Identity = 51/145 (35.17%), Postives = 86/145 (59.31%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMT 728
            +L  E+I + K  F +FD + DG ++  EL   +R+LG   + A+ ++++ E+  +G   IDF  FL  +    K++ D+ EE+ + F+ FD    G+IS   L+ +   L   L+  ++ +MI EADI+GDG V+ EEFV++MT
Sbjct:    4 QLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDT-DSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMT 147          
BLAST of Caltractin vs. Ensembl Medaka
Match: calm1b (calmodulin [Source:NCBI gene;Acc:100049419])

HSP 1 Score: 88.1965 bits (217), Expect = 2.660e-21
Identity = 51/145 (35.17%), Postives = 86/145 (59.31%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMT 728
            +L  E+I + K  F +FD + DG ++  EL   +R+LG   + A+ ++++ E+  +G   IDF  FL  +    K++ D+ EE+ + F+ FD    G+IS   L+ +   L   L+  ++ +MI EADI+GDG V+ EEFV++MT
Sbjct:    4 QLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDT-DSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMT 147          
BLAST of Caltractin vs. Planmine SMEST
Match: SMESG000029908.1 (SMESG000029908.1)

HSP 1 Score: 397.127 bits (1019), Expect = 2.273e-141
Identity = 196/198 (98.99%), Postives = 196/198 (98.99%), Query Frame = 3
Query:  153 VLSEGYLIKDPKPIYVPLPGENLLKVLTTHPVGEKEVYNMVISSKALKELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELMELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNLFK 746
            VLSEGYLIKDPKPIYVPLPGENLLKVLTTHPVGEKEVYNMVISSKALKELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELMELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLS ELNVPLSVHQLQ MINEADINGDGVVSEEEFVKIMTQTNLFK
Sbjct:   23 VLSEGYLIKDPKPIYVPLPGENLLKVLTTHPVGEKEVYNMVISSKALKELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELMELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSVELNVPLSVHQLQNMINEADINGDGVVSEEEFVKIMTQTNLFK 220          
BLAST of Caltractin vs. Planmine SMEST
Match: SMESG000015458.1 (SMESG000015458.1)

HSP 1 Score: 92.4337 bits (228), Expect = 8.343e-23
Identity = 53/150 (35.33%), Postives = 94/150 (62.67%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNLF 743
            EL  E+ ++++  FD+FD++  G + I EL++++RALGF+    +  +L+ +    G  II+F+ FL+ + +   E +DT EE+++ FK FD  +T    L   K     +   L+  +LQ+MI+EAD + DG ++E+EF++IM +TNL+
Sbjct:   25 ELTEEQKQEVREAFDLFDADGSGTIDIKELKVAMRALGFEPKKEEVRKLIADYDIEGKGIIEFSDFLSMMTQKMAE-KDTKEEILKAFKLFDDDETEKFLL-KFKKSCKRIRRNLTDEELQEMIDEADRDNDGEINEQEFLRIMKKTNLY 172          
BLAST of Caltractin vs. Planmine SMEST
Match: SMESG000034095.1 (SMESG000034095.1)

HSP 1 Score: 88.1965 bits (217), Expect = 1.904e-21
Identity = 52/145 (35.86%), Postives = 85/145 (58.62%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMT 728
            +L  E+I + K  F +FD + DG ++  EL   +R+LG   + A+ ++++ E+  +G   IDF  FL  +    K++ D+ EE+ + F+ FD    GFIS   L+ +   L   L+  ++ +MI EADI+GDG V+ EEFV +MT
Sbjct:    4 QLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDT-DSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVTMMT 147          
BLAST of Caltractin vs. Planmine SMEST
Match: SMESG000010769.1 (SMESG000010769.1)

HSP 1 Score: 88.1965 bits (217), Expect = 1.904e-21
Identity = 52/145 (35.86%), Postives = 85/145 (58.62%), Query Frame = 3
Query:  297 ELIPEEIKDLKMVFDVFDSNNDGFLSIPELRMSLRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMT 728
            +L  E+I + K  F +FD + DG ++  EL   +R+LG   + A+ ++++ E+  +G   IDF  FL  +    K++ D+ EE+ + F+ FD    GFIS   L+ +   L   L+  ++ +MI EADI+GDG V+ EEFV +MT
Sbjct:    4 QLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDT-DSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVTMMT 147          
BLAST of Caltractin vs. Planmine SMEST
Match: SMESG000052182.1 (SMESG000052182.1)

HSP 1 Score: 87.0409 bits (214), Expect = 2.109e-21
Identity = 48/117 (41.03%), Postives = 78/117 (66.67%), Query Frame = 3
Query:  396 LRALGFKFSSAQAEELM-ELSTNGIKIIDFNGFLNCVIENQKESRDTFEEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADINGDGVVSEEEFVKIMTQTNLF 743
            +RALGF+    +  +L+ +    G  II+F+ FL+ + +   E +DT EE+++ FK FD  +TG IS  NLK ++ EL   L+  +LQ+MI+EAD + DG ++E+EF++IM +TNL+
Sbjct:    1 MRALGFEPKKEEVRKLIADYDIEGKGIIEFSDFLSMMTQKMAE-KDTKEEILKAFKLFDDDETGKISFKNLKRVAKELGENLTDEELQEMIDEADRDNDGEINEQEFLRIMKKTNLY 116          
The following BLAST results are available for this feature:
BLAST of Caltractin vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CETN21.987e-3037.58centrin 2 [Source:HGNC Symbol;Acc:HGNC:1867][more]
CETN19.552e-2938.67centrin 1 [Source:HGNC Symbol;Acc:HGNC:1866][more]
CETN32.294e-2235.48centrin 3 [Source:HGNC Symbol;Acc:HGNC:1868][more]
CALM14.685e-2135.17calmodulin 1 [Source:HGNC Symbol;Acc:HGNC:1442][more]
CALM24.964e-2135.17calmodulin 2 [Source:HGNC Symbol;Acc:HGNC:1445][more]
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BLAST of Caltractin vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
cmd-11.816e-2135.86Calmodulin [Source:UniProtKB/Swiss-Prot;Acc:O1630... [more]
cal-12.855e-1629.87Calmodulin-like protein [Source:UniProtKB/Swiss-P... [more]
cal-15.996e-1629.87Calmodulin-like protein [Source:UniProtKB/Swiss-P... [more]
cmd-11.464e-1436.61Calmodulin [Source:UniProtKB/Swiss-Prot;Acc:O1630... [more]
cal-83.708e-1333.09CALmodulin related genes [Source:UniProtKB/TrEMBL... [more]
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BLAST of Caltractin vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG174934.011e-2638.03gene:FBgn0040010 transcript:FBtr0332630[more]
CG174934.011e-2638.03gene:FBgn0040010 transcript:FBtr0332632[more]
CG174934.011e-2638.03gene:FBgn0040010 transcript:FBtr0332631[more]
CG318025.710e-2638.18gene:FBgn0051802 transcript:FBtr0081058[more]
Cam2.403e-2135.86gene:FBgn0000253 transcript:FBtr0304964[more]
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BLAST of Caltractin vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
cetn43.750e-2937.33centrin 4 [Source:ZFIN;Acc:ZDB-GENE-030826-21][more]
cetn23.523e-2835.33centrin, EF-hand protein, 2 [Source:ZFIN;Acc:ZDB-G... [more]
cetn31.043e-2135.10centrin 3 [Source:ZFIN;Acc:ZDB-GENE-050522-152][more]
cetn31.071e-2135.57centrin 3 [Source:ZFIN;Acc:ZDB-GENE-050522-152][more]
calm2a2.786e-2135.17calmodulin 2a (phosphorylase kinase, delta) [Sourc... [more]
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BLAST of Caltractin vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
dtl2.558e-3036.69denticleless E3 ubiquitin protein ligase homolog [... [more]
dtl1.044e-2938.16denticleless E3 ubiquitin protein ligase homolog [... [more]
cetn14.605e-2936.67centrin 1 [Source:NCBI gene;Acc:549903][more]
cetn15.608e-2936.67centrin 1 [Source:NCBI gene;Acc:549903][more]
rab384.365e-2235.06RAB38, member RAS oncogene family [Source:Xenbase;... [more]
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BLAST of Caltractin vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Cetn18.278e-3140.00centrin 1 [Source:MGI Symbol;Acc:MGI:1347086][more]
Cetn18.278e-3140.00centrin 1 [Source:MGI Symbol;Acc:MGI:1347086][more]
Cetn18.278e-3140.00centrin 1 [Source:MGI Symbol;Acc:MGI:1347086][more]
Cetn21.651e-2938.00centrin 2 [Source:MGI Symbol;Acc:MGI:1347085][more]
Cetn21.651e-2938.00centrin 2 [Source:MGI Symbol;Acc:MGI:1347085][more]
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BLAST of Caltractin vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P41209|CETN1_MOUSE5.791e-3040.00Centrin-1 OS=Mus musculus OX=10090 GN=Cetn1 PE=1 S... [more]
sp|P41208|CETN2_HUMAN9.542e-3037.58Centrin-2 OS=Homo sapiens OX=9606 GN=CETN2 PE=1 SV... [more]
sp|Q32LE3|CETN1_BOVIN1.218e-2938.67Centrin-1 OS=Bos taurus OX=9913 GN=CETN1 PE=2 SV=1[more]
sp|P43646|CATR_TETST2.545e-2939.60Caltractin (Fragment) OS=Tetraselmis striata OX=31... [more]
sp|Q2TBN3|CETN2_BOVIN3.306e-2938.67Centrin-2 OS=Bos taurus OX=9913 GN=CETN2 PE=2 SV=1[more]
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BLAST of Caltractin vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267E0132.059e-5550.26Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A1I8G6T32.116e-5551.34Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267FW942.997e-5550.26Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A1I8GNX63.618e-5550.26Uncharacterized protein OS=Macrostomum lignano OX=... [more]
V4B6X71.542e-5448.68Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
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BLAST of Caltractin vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
cetn42.586e-2936.67caltractin [Source:NCBI gene;Acc:103037928][more]
cetn42.951e-2936.67caltractin [Source:NCBI gene;Acc:103037928][more]
cetn21.879e-2736.00centrin-1 [Source:NCBI gene;Acc:103028142][more]
cetn38.854e-2336.00centrin 3 [Source:NCBI gene;Acc:103024517][more]
calm3a2.676e-2135.17calmodulin [Source:NCBI gene;Acc:103037741][more]
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BLAST of Caltractin vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CETN13.846e-3037.33centrin 1 [Source:HGNC Symbol;Acc:HGNC:1866][more]
ENSPMAT00000006008.11.704e-2235.17pep scaffold:Pmarinus_7.0:GL476713:142259:146509:-... [more]
CALML34.790e-2235.17calmodulin like 3 [Source:HGNC Symbol;Acc:HGNC:145... [more]
CALM35.429e-2235.17calmodulin 3 [Source:HGNC Symbol;Acc:HGNC:1449][more]
cetn35.571e-2232.41centrin 3 [Source:ZFIN;Acc:ZDB-GENE-050522-152][more]
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BLAST of Caltractin vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2
Match NameE-valueIdentityDescription
CDC314.004e-2334.69Calcium-binding component of the spindle pole body... [more]
CMD18.190e-1530.34Calmodulin; Ca2+ binding protein that regulates Ca... [more]
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BLAST of Caltractin vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO418591.002e-3540.76Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO407756.968e-3038.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO465181.430e-2236.55Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO449446.410e-2235.71Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO466067.973e-2035.25Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Caltractin vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
cetn27.727e-2836.00caltractin [Source:NCBI gene;Acc:101170769][more]
cetn27.727e-2836.00caltractin [Source:NCBI gene;Acc:101170769][more]
calm2a2.660e-2135.17calmodulin 2 [Source:NCBI gene;Acc:100049420][more]
ENSORLT00000008599.22.660e-2135.17calmodulin 3 [Source:NCBI gene;Acc:100049421][more]
calm1b2.660e-2135.17calmodulin [Source:NCBI gene;Acc:100049419][more]
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BLAST of Caltractin vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000029908.12.273e-14198.99SMESG000029908.1[more]
SMESG000015458.18.343e-2335.33SMESG000015458.1[more]
SMESG000034095.11.904e-2135.86SMESG000034095.1[more]
SMESG000010769.11.904e-2135.86SMESG000010769.1[more]
SMESG000052182.12.109e-2141.03SMESG000052182.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30000846 ID=SMED30000846|Name=Caltractin|organism=Schmidtea mediterranea sexual|type=transcript|length=849bp
AGCAACGCTATTTCAATATTTATAATTAAATTGATAAAATAGTTATTCAT
ATATTAAATCTATATTCTATTTGAAATTTCAATAAAATGCCGAAAAAAGG
TGGTAAAAAAACGGGTAGAAAATCTACTAAAACTAAATCTCAGACAAAGT
CAGTCTTATCTGAAGGATATTTAATAAAAGATCCAAAACCTATTTATGTA
CCACTTCCTGGAGAAAATCTGCTAAAGGTCCTAACAACACATCCAGTTGG
TGAAAAGGAAGTTTATAATATGGTAATTAGTTCGAAAGCTCTGAAAGAAT
TAATTCCCGAAGAAATAAAAGATTTGAAAATGGTTTTCGATGTTTTCGAT
TCGAATAATGACGGATTCCTTTCGATTCCTGAATTGCGAATGAGTCTCAG
AGCTTTGGGATTCAAATTCTCGTCGGCTCAAGCCGAGGAATTGATGGAAT
TATCCACCAATGGAATCAAAATCATTGATTTCAATGGATTTCTAAATTGC
GTAATCGAAAATCAAAAAGAATCGAGAGATACGTTCGAGGAAATGATGCA
AGGATTCAAATATTTCGACTTGCATGATACTGGCTTTATTAGCTTGGACA
ATCTCAAAACTTTGTCCGCAGAACTCAACGTACCTCTGTCGGTGCACCAA
TTGCAGAAGATGATAAATGAGGCTGATATCAATGGAGATGGGGTTGTTTC
TGAGGAGGAATTCGTGAAAATAATGACACAAACTAATTTGTTCAAATAAA
TTTACTTTCTAATATCTCTTTTCTAAATTTGTTTTAATGCTGAAAATTTT
TGTCAAATAGAAAACATTGATTTGTTGTCGAATACTAAATGGAGATGAA
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protein sequence of SMED30000846-orf-1

>SMED30000846-orf-1 ID=SMED30000846-orf-1|Name=SMED30000846-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=221bp
MPKKGGKKTGRKSTKTKSQTKSVLSEGYLIKDPKPIYVPLPGENLLKVLT
THPVGEKEVYNMVISSKALKELIPEEIKDLKMVFDVFDSNNDGFLSIPEL
RMSLRALGFKFSSAQAEELMELSTNGIKIIDFNGFLNCVIENQKESRDTF
EEMMQGFKYFDLHDTGFISLDNLKTLSAELNVPLSVHQLQKMINEADING
DGVVSEEEFVKIMTQTNLFK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: molecular function
TermDefinition
GO:0005509calcium ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 152..180
e-value: 120.0
score: 1.0
coord: 188..216
e-value: 2.8E-6
score: 36.9
coord: 79..107
e-value: 0.0047
score: 26.1
IPR002048EF-hand domainPFAMPF13405EF-hand_6coord: 80..108
e-value: 3.8E-5
score: 23.3
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 150..213
e-value: 4.6E-11
score: 43.0
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 148..183
score: 7.428
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 75..110
score: 14.011
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 184..219
score: 12.895
IPR002048EF-hand domainCDDcd00051EFhcoord: 152..214
e-value: 1.18269E-11
score: 56.0169
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 70..134
e-value: 4.6E-13
score: 51.3
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 135..219
e-value: 1.0E-18
score: 69.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..15
NoneNo IPR availablePANTHERPTHR23050:SF329coord: 62..219
NoneNo IPR availablePANTHERPTHR23050CALCIUM BINDING PROTEINcoord: 62..219
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 88..100
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 197..209
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 74..215