SMED30000834

Overview
NameSMED30000834
Smed IDSMED30000834
Length (bp)2097
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30000834 (SMED30000834) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30000834 (SMED30000834) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30000834 (SMED30000834) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30000834 (SMED30000834) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30000834

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30000834SMESG000070604.1 SMESG000070322.1 SMESG000017548.1 SmedASXL_009478SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30000834SMESG000070604.1 SMESG000070322.1 SMESG000017548.1 SmedASXL_009478SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
nervous systemSMED30000834SMESG000070604.1 SMESG000070322.1 SMESG000017548.1 dd_Smed_v4_2059_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30000834SMESG000070604.1 SMESG000070322.1 SMESG000017548.1 dd_Smed_v4_2059_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30000834SMESG000070604.1 SMESG000070322.1 SMESG000017548.1 dd_Smed_v4_2059_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30000834SMESG000070604.1 SMESG000070322.1 SMESG000017548.1 dd_Smed_v4_2059_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30000834SMESG000070604.1 SMESG000070322.1 SMESG000017548.1 dd_Smed_v4_2059_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30000834SMESG000017548.1 SMESG000070604.1 SMESG000070322.1 dd_Smed_v6_2059_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30000834 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.004746:18204..21994 +8409
Homology
[Close]
BLAST of SMED30000834 vs. Planmine SMEST
Match: SMESG000070604.1 (SMESG000070604.1)

HSP 1 Score: 1268.06 bits (3280), Expect = 0.000e+0
Identity = 627/643 (97.51%), Postives = 638/643 (99.22%), Query Frame = 2 
Query:   29 MNTDLLYVSNIPSSMKPVLLLHELENKWSRMLYLENSFSGMRDALIYNPSSSMQQFYNGVQKLRSKVFDDNKCLSVSYVWFPPPNVFITTMIPDGASILSYIFVENLHKEGNVAELTADLPLQQHDIKLWDIPERQDKCLIIKLPPSSNTAMRSYVLGTLRNRKIKGFFPQVYEMIISDTPELSKVMVPKTIQTTESSESLMANFLFLHNIPSKLNVKSLVDSTKIDNIKGCRVFFLETINKLKMGFIYIPKTLPYNKFIGLFEKLRTNEYTQDDGTSSRIDLDEVWFQPADADPPNLTDEEQRSLVPPEFVLMNYLYLHNIPPNFVASQLLMGSPFAVKLYVFTVNDLSTQIGFVVLRGLSPENCQMLIEHFRNLVIGGLPILVDEIYLKCYNLADIETRDAEYIPRPQSTRVYNKRSIDNSTNGAVEXXXXXXXXXXXXXXPPEGWPLMKSEFICLSNLPNSMNRSAILFNIGVAVGGCVLTTCSDGKPYAFVYLPDCEPTLWENVISKLASKNIQGNFMDIHEIYLQSNDDCMAIIGNEYRPSPFIFIYNFPSTPVEDERSLLYHELIFDIAFMDTDNGGRCGLATLYNGTEEGQIIDCLNHIRTIKIQGKPLFAEIMWFADVKVDVDQPPEKKVRCLME 1957
            MNTDLLYVSNIPS+MKPVLLLHELENKWSRMLYLENSFSG+RDALIYNP SS+Q FYNGVQ+LRSKVFDDNKCLSVSYVWFPPPNVFITTMIPDGASILSYIFVEN HKEGNVAELTADLPLQQHDIK+WDIPERQDKCL+IKLP SS+TAMRSYVLGTLRNRK+KG+FP+VYEMIIS+T ELSKVMVPKTIQTTESSESLMANFLFLHNIPSKLNVKSLVDSTKIDNIKGCRVFFLETINKLKMGFIYIPKTLPYNKFIGLFEKLRTNEYTQDDGTSSRIDLDEVWFQPADADPPNLTDEEQRSLVPPEFVLMNYLYLHNIPPNFVASQLLMGSPFAVKLYVFTVNDLSTQIGFVVLRGLSPENCQMLIEHFRNLVIGGLPILVDEIYLKCYNLADIETRDAEYIPRPQSTRVYNKRSIDNSTNGAVESTKRRISKIKLSKSPPEGWPLMKSEFICLSNLPNSMNRSAILFNIGVAVGGCVLTTCSDGKPYAFVYLPDCEPTLWENVISKLASKNIQGNFMDIHEIYLQSNDDCMAIIGNEYRPSPFIFIYNFPSTPVEDERSLLYHELIFDIAFMDTDNGGRCGLATLYNGTEEGQIIDCLNHIRTIKIQGKPLFAEIMWFADVKVDVDQPPEKKVRCLME
Sbjct:    1 MNTDLLYVSNIPSTMKPVLLLHELENKWSRMLYLENSFSGVRDALIYNPLSSIQIFYNGVQRLRSKVFDDNKCLSVSYVWFPPPNVFITTMIPDGASILSYIFVENFHKEGNVAELTADLPLQQHDIKIWDIPERQDKCLVIKLPLSSSTAMRSYVLGTLRNRKVKGYFPRVYEMIISNTIELSKVMVPKTIQTTESSESLMANFLFLHNIPSKLNVKSLVDSTKIDNIKGCRVFFLETINKLKMGFIYIPKTLPYNKFIGLFEKLRTNEYTQDDGTSSRIDLDEVWFQPADADPPNLTDEEQRSLVPPEFVLMNYLYLHNIPPNFVASQLLMGSPFAVKLYVFTVNDLSTQIGFVVLRGLSPENCQMLIEHFRNLVIGGLPILVDEIYLKCYNLADIETRDAEYIPRPQSTRVYNKRSIDNSTNGAVESTKRRISKIKLSKSPPEGWPLMKSEFICLSNLPNSMNRSAILFNIGVAVGGCVLTTCSDGKPYAFVYLPDCEPTLWENVISKLASKNIQGNFMDIHEIYLQSNDDCMAIIGNEYRPSPFIFIYNFPSTPVEDERSLLYHELIFDIAFMDTDNGGRCGLATLYNGTEEGQIIDCLNHIRTIKIQGKPLFAEIMWFADVKVDVDQPPEKKVRCLME 643
[Close]
BLAST of SMED30000834 vs. Planmine SMEST
Match: SMESG000070322.1 (SMESG000070322.1)

HSP 1 Score: 1266.91 bits (3277), Expect = 0.000e+0
Identity = 626/643 (97.36%), Postives = 637/643 (99.07%), Query Frame = 2 
Query:   29 MNTDLLYVSNIPSSMKPVLLLHELENKWSRMLYLENSFSGMRDALIYNPSSSMQQFYNGVQKLRSKVFDDNKCLSVSYVWFPPPNVFITTMIPDGASILSYIFVENLHKEGNVAELTADLPLQQHDIKLWDIPERQDKCLIIKLPPSSNTAMRSYVLGTLRNRKIKGFFPQVYEMIISDTPELSKVMVPKTIQTTESSESLMANFLFLHNIPSKLNVKSLVDSTKIDNIKGCRVFFLETINKLKMGFIYIPKTLPYNKFIGLFEKLRTNEYTQDDGTSSRIDLDEVWFQPADADPPNLTDEEQRSLVPPEFVLMNYLYLHNIPPNFVASQLLMGSPFAVKLYVFTVNDLSTQIGFVVLRGLSPENCQMLIEHFRNLVIGGLPILVDEIYLKCYNLADIETRDAEYIPRPQSTRVYNKRSIDNSTNGAVEXXXXXXXXXXXXXXPPEGWPLMKSEFICLSNLPNSMNRSAILFNIGVAVGGCVLTTCSDGKPYAFVYLPDCEPTLWENVISKLASKNIQGNFMDIHEIYLQSNDDCMAIIGNEYRPSPFIFIYNFPSTPVEDERSLLYHELIFDIAFMDTDNGGRCGLATLYNGTEEGQIIDCLNHIRTIKIQGKPLFAEIMWFADVKVDVDQPPEKKVRCLME 1957
            MNTDLLYVSNIPS+MKPVLLLHELENKWSRMLYLENSFSG+RDALIYNP SS+Q FYNGVQ+LRSKVFDDNKCLSVSYVWFPPPNVFITTMIPDGASILSYIFVEN HKEGNVAELTADLPLQQHDIK+WDIPERQDKCL+IKLP SS+TAMRSYVLGTLRNRK+KG+FP+VYEMIIS+T ELSKVMVPKTIQTTESSESLMANFLFLHNIPSKLNVKSLVDSTKIDNIKGCRVFFLETINKLKMGFIYIPKTLPYNKFIGLFEKLRTNEYTQDDGTSSRIDLDEVWFQPADADPPNLTDEEQRSLVPPEFVLMNYLYLHNIPPNFVASQLLMGSPFAVKLYVFTVNDLSTQIGFVVLRGLSPENCQMLIEHFRNLVIGGLPILVDEIYLKCYNLADIETRDAEYIPRPQSTRVYNKRSIDNSTNGAVESTKRRISKIKLSKSPPEGWPLMKSEFICLSNLPNSMNRSAILFNIGVAVGGCVLTTCSDGKPYAFVYLPDCEPTLWENVI KLASKNIQGNFMDIHEIYLQSNDDCMAIIGNEYRPSPFIFIYNFPSTPVEDERSLLYHELIFDIAFMDTDNGGRCGLATLYNGTEEGQIIDCLNHIRTIKIQGKPLFAEIMWFADVKVDVDQPPEKKVRCLME
Sbjct:    1 MNTDLLYVSNIPSTMKPVLLLHELENKWSRMLYLENSFSGVRDALIYNPLSSIQIFYNGVQRLRSKVFDDNKCLSVSYVWFPPPNVFITTMIPDGASILSYIFVENFHKEGNVAELTADLPLQQHDIKIWDIPERQDKCLVIKLPLSSSTAMRSYVLGTLRNRKVKGYFPRVYEMIISNTIELSKVMVPKTIQTTESSESLMANFLFLHNIPSKLNVKSLVDSTKIDNIKGCRVFFLETINKLKMGFIYIPKTLPYNKFIGLFEKLRTNEYTQDDGTSSRIDLDEVWFQPADADPPNLTDEEQRSLVPPEFVLMNYLYLHNIPPNFVASQLLMGSPFAVKLYVFTVNDLSTQIGFVVLRGLSPENCQMLIEHFRNLVIGGLPILVDEIYLKCYNLADIETRDAEYIPRPQSTRVYNKRSIDNSTNGAVESTKRRISKIKLSKSPPEGWPLMKSEFICLSNLPNSMNRSAILFNIGVAVGGCVLTTCSDGKPYAFVYLPDCEPTLWENVIGKLASKNIQGNFMDIHEIYLQSNDDCMAIIGNEYRPSPFIFIYNFPSTPVEDERSLLYHELIFDIAFMDTDNGGRCGLATLYNGTEEGQIIDCLNHIRTIKIQGKPLFAEIMWFADVKVDVDQPPEKKVRCLME 643
[Close]
BLAST of SMED30000834 vs. Planmine SMEST
Match: SMESG000017548.1 (SMESG000017548.1)

HSP 1 Score: 1174.84 bits (3038), Expect = 0.000e+0
Identity = 585/643 (90.98%), Postives = 594/643 (92.38%), Query Frame = 2 
Query:   29 MNTDLLYVSNIPSSMKPVLLLHELENKWSRMLYLENSFSGMRDALIYNPSSSMQQFYNGVQKLRSKVFDDNKCLSVSYVWFPPPNVFITTMIPDGASILSYIFVENLHKEGNVAELTADLPLQQHDIKLWDIPERQDKCLIIKLPPSSNTAMRSYVLGTLRNRKIKGFFPQVYEMIISDTPELSKVMVPKTIQTTESSESLMANFLFLHNIPSKLNVKSLVDSTKIDNIKGCRVFFLETINKLKMGFIYIPKTLPYNKFIGLFEKLRTNEYTQDDGTSSRIDLDEVWFQPADADPPNLTDEEQRSLVPPEFVLMNYLYLHNIPPNFVASQLLMGSPFAVKLYVFTVNDLSTQIGFVVLRGLSPENCQMLIEHFRNLVIGGLPILVDEIYLKCYNLADIETRDAEYIPRPQSTRVYNKRSIDNSTNGAVEXXXXXXXXXXXXXXPPEGWPLMKSEFICLSNLPNSMNRSAILFNIGVAVGGCVLTTCSDGKPYAFVYLPDCEPTLWENVISKLASKNIQGNFMDIHEIYLQSNDDCMAIIGNEYRPSPFIFIYNFPSTPVEDERSLLYHELIFDIAFMDTDNGGRCGLATLYNGTEEGQIIDCLNHIRTIKIQGKPLFAEIMWFADVKVDVDQPPEKKVRCLME 1957
            MNTDLLYVSNIPSSMKPVLLLHELENKWSRMLYLENSFSGMRDALIYNPSSSMQQFYNGVQKLRSKVFDDNKCLSVSYVWFPPPNVFITTMIPDGASILSYIFVENLHKEGNVAELTADLPLQQHDIKLWDIPERQDKCLIIKLPPSSNTAMRSYVLGTLRNRKIKGFFPQVYEMIISDTPELSKVMVPKTIQTTESSESLMANFLFLHNIPSKLNVKSLVDSTKIDNIKGCRVFFLETINKLKMGFIYIPKTLPYNKFIGLFEKLRTNEYTQDDGTSSRIDLDEVWFQPADADPPNLTDEEQRSLVPPEFVLMNYLYLHNIPPNFVASQLLMGSPFAVKLYVFTVNDLSTQIGFVVLRGLSPENCQMLIEHFRNLVIGGLPILVDEIYLKCYNLADIETRDAEYIPRP       +  I  +    + +   ++    L  S P+GWPLMKSEFICLSNLPNSMNRSAILFNIGVAVGGCVLTTCSDGKPYAFVYLPDCEPTLWENVI KLASK     F  I  I+ +S    M I       SPFIFIYNFPSTPVEDERSLLYHELIFDIAFMDTDNGGRCGLATLYNGTEEGQIIDCLNHIRTIKIQGKPLFAEIMWFADVKVDVDQPPEKKVRCLME
Sbjct:    1 MNTDLLYVSNIPSSMKPVLLLHELENKWSRMLYLENSFSGMRDALIYNPSSSMQQFYNGVQKLRSKVFDDNKCLSVSYVWFPPPNVFITTMIPDGASILSYIFVENLHKEGNVAELTADLPLQQHDIKLWDIPERQDKCLIIKLPPSSNTAMRSYVLGTLRNRKIKGFFPQVYEMIISDTPELSKVMVPKTIQTTESSESLMANFLFLHNIPSKLNVKSLVDSTKIDNIKGCRVFFLETINKLKMGFIYIPKTLPYNKFIGLFEKLRTNEYTQDDGTSSRIDLDEVWFQPADADPPNLTDEEQRSLVPPEFVLMNYLYLHNIPPNFVASQLLMGSPFAVKLYVFTVNDLSTQIGFVVLRGLSPENCQMLIEHFRNLVIGGLPILVDEIYLKCYNLADIETRDAEYIPRPHPL----ESIIREALTIQLMALSNQLKDESLKLSCPKGWPLMKSEFICLSNLPNSMNRSAILFNIGVAVGGCVLTTCSDGKPYAFVYLPDCEPTLWENVIGKLASK----TFKAISWIFTRSTCSRMTIAWQLLAMSPFIFIYNFPSTPVEDERSLLYHELIFDIAFMDTDNGGRCGLATLYNGTEEGQIIDCLNHIRTIKIQGKPLFAEIMWFADVKVDVDQPPEKKVRCLME 635
[Close]
BLAST of SMED30000834 vs. Planmine SMEST
Match: SMESG000017548.1 (SMESG000017548.1)

HSP 1 Score: 1079.32 bits (2790), Expect = 0.000e+0
Identity = 549/643 (85.38%), Postives = 558/643 (86.78%), Query Frame = 2 
Query:   29 MNTDLLYVSNIPSSMKPVLLLHELENKWSRMLYLENSFSGMRDALIYNPSSSMQQFYNGVQKLRSKVFDDNKCLSVSYVWFPPPNVFITTMIPDGASILSYIFVENLHKEGNVAELTADLPLQQHDIKLWDIPERQDKCLIIKLPPSSNTAMRSYVLGTLRNRKIKGFFPQVYEMIISDTPELSKVMVPKTIQTTESSESLMANFLFLHNIPSKLNVKSLVDSTKIDNIKGCRVFFLETINKLKMGFIYIPKTLPYNKFIGLFEKLRTNEYTQDDGTSSRIDLDEVWFQPADADPPNLTDEEQRSLVPPEFVLMNYLYLHNIPPNFVASQLLMGSPFAVKLYVFTVNDLSTQIGFVVLRGLSPENCQMLIEHFRNLVIGGLPILVDEIYLKCYNLADIETRDAEYIPRPQSTRVYNKRSIDNSTNGAVEXXXXXXXXXXXXXXPPEGWPLMKSEFICLSNLPNSMNRSAILFNIGVAVGGCVLTTCSDGKPYAFVYLPDCEPTLWENVISKLASKNIQGNFMDIHEIYLQSNDDCMAIIGNEYRPSPFIFIYNFPSTPVEDERSLLYHELIFDIAFMDTDNGGRCGLATLYNGTEEGQIIDCLNHIRTIKIQGKPLFAEIMWFADVKVDVDQPPEKKVRCLME 1957
            MNTDLLYVSNIPSSMKPVLLLHELENKWSRMLYLENSFSGMRDALIYNPSSSMQQFYNGVQKLRSKVFDDNKCLSVSYVWFPPPNVFITTMIPDGASILSYIFVENLHKEGNVAELTADLPLQQHDIKLWDIPERQDKCLIIKLPPSSNTAMRSYVLGTLRNRKIKGFFPQVYEMIISDTPELSKVMVPKTIQTTESSESLMANFLFLHNIPSKLNVKSLVDSTKIDNIKGCRVFFLETINKLKMGFIYIPKTLPYNKFIGLFEKLRTNEYTQDDGTSSRIDLDEVWFQPADADPPNLTDEEQRSLVPPEFVLMNYLYLHNIPPNFVASQLLMGSPFAVKLYVFTVNDLSTQIGFVVLRGLSPENCQMLIEHFRNLVIGGLPILVDEIYLKCYNLADIETRDAEYIPRP       +  I  +    + +   ++    L  S P+GWPLMKSEFICLSNLPNSMNRSAILFNIGVAVGGCVLTTCSDGKPYAFVYLPDCEPTLWENVI KLASK     F  I  I+ +S    M I                          LL                 RCGLATLYNGTEEGQIIDCLNHIRTIKIQGKPLFAEIMWFADVKVDVDQPPEKKVRCLME
Sbjct:    1 MNTDLLYVSNIPSSMKPVLLLHELENKWSRMLYLENSFSGMRDALIYNPSSSMQQFYNGVQKLRSKVFDDNKCLSVSYVWFPPPNVFITTMIPDGASILSYIFVENLHKEGNVAELTADLPLQQHDIKLWDIPERQDKCLIIKLPPSSNTAMRSYVLGTLRNRKIKGFFPQVYEMIISDTPELSKVMVPKTIQTTESSESLMANFLFLHNIPSKLNVKSLVDSTKIDNIKGCRVFFLETINKLKMGFIYIPKTLPYNKFIGLFEKLRTNEYTQDDGTSSRIDLDEVWFQPADADPPNLTDEEQRSLVPPEFVLMNYLYLHNIPPNFVASQLLMGSPFAVKLYVFTVNDLSTQIGFVVLRGLSPENCQMLIEHFRNLVIGGLPILVDEIYLKCYNLADIETRDAEYIPRPHPL----ESIIREALTIQLMALSNQLKDESLKLSCPKGWPLMKSEFICLSNLPNSMNRSAILFNIGVAVGGCVLTTCSDGKPYAFVYLPDCEPTLWENVIGKLASK----TFKAISWIFTRSTCSRMTIAWQ-----------------------LL---------------AMRCGLATLYNGTEEGQIIDCLNHIRTIKIQGKPLFAEIMWFADVKVDVDQPPEKKVRCLME 597

HSP 2 Score: 99.3673 bits (246), Expect = 1.833e-21
Identity = 71/150 (47.33%), Postives = 83/150 (55.33%), Query Frame = 3 
Query: 1260 PLESIIREALTIQLMALSNQLKDESLKLSCP--------------NLPQ---KDGL**NLSLFAYPIYRTR*IDQQFYSISEWQLAAVY*RHVLMASRTHLSIC-PTV-SRLCGRMSSAN*HRKTFKAISWIFTRSTCSRMTIAWQLLAM 1652
            PLESIIREALTIQLMALSNQLKDESLKLSCP              NLP    +  +  N+ +       T   D + Y+                    +L  C PT+   + G+++S     KTFKAISWIFTRSTCSRMTIAWQLLAM
Sbjct:  411 PLESIIREALTIQLMALSNQLKDESLKLSCPKGWPLMKSEFICLSNLPNSMNRSAILFNIGVAVGGCVLTTCSDGKPYAF------------------VYLPDCEPTLWENVIGKLAS-----KTFKAISWIFTRSTCSRMTIAWQLLAM 537
[Close]
BLAST of SMED30000834 vs. Planmine SMEST
Match: SMESG000070344.1 (SMESG000070344.1)

HSP 1 Score: 965.681 bits (2495), Expect = 0.000e+0
Identity = 471/644 (73.14%), Postives = 551/644 (85.56%), Query Frame = 2 
Query:   29 MNTDLLYVSNIPSSMKPVLLLHELENKWSRMLYLENSFSGMRDALIYNPSSSMQQFYNGVQKLRSKVFDDNKCLSVSYVWFPPPNVFITTMIPDGASILSYIFVENLHKEGNVAELTADLPLQQHDIKLWDIPERQDKCLIIKLPPSSNTAMRSYVLGTLRNRKIKGFFPQVYEMIISDTPELSKVMVPKTIQTTESSESLMANFLFLHNIPSKLNVKSLVDSTKIDNIKGCRVFFLETINKLKMGFIYIPKTLPYNKFIGLFEKLRTNEYTQDDGTSSRIDLDEVWFQPADADPPNLTDEEQRSLVPPEFVLMNYLYLHNIPPNFVASQLLMGSPFAVKLYVFTVNDLSTQIGFVVLRGLSPENCQMLIEHFRNLVIGGLPILVDEIYLKCYNLADIETRDAEYIPR-----PQSTRVYNKRSIDNSTNGAVEXXXXXXXXXXXXXXPPEGWPLMKSEFICLSNLPNSMNRSAILFNIGVAVGGCVLTTCSDGKPYAFVYLPDCEPTLWENVISKLASKNIQGNFMDIHEIYLQSNDDCMAIIGNEYRPSPFIFIYNFPSTPVEDERSLLYHELIFDIAFMDTDNGGRCGLATLYNGTEEGQIIDCLNHIRTIKIQGKPLFAEIMWFADVKVDVDQPPEKKVR 1945
            MNTDL+YVSNIPSSMKPVLLLHELENKWSRMLYLENSFSGMRDA IYNP+ S+  F+ GVQKLR KVF+D+K LSVSYVWFPPPNVFITTMIPDGAS+LSY+FVENL KE NVAELTA LP QQHDIKLWDIPERQDKCL+IKLP SSNT+MRSYVLGTLRNRK+KG+FP VYE+IISDTPELSKVMVPKT   TESSES MANFLFL+NIPSKLNVKSLVDSTK DNIKGCRVFF+ET+NK K+GF YIPKTLPYNKF+ LFEKL++ EY  DDGT+ +IDL+EVWFQP +A+PP LT++E  S  PPEF+L  YLYLHNIPPN+VASQLLMG+P  VKLYVF+VNDLSTQIGFVVLRGL+PE CQ++I+HFR+LV+GGLPILVDEIYLKCY+L DI +RD E+IPR     P++     KR ++NS NG +ES +R IS IKLSK P EGW LMKS+FICL+NLPN+++RSAI+F+IG  VGGCVL + S+GKP+AFVYLPDCE  LWE ++SKLA+K I GN++DIHE+YLQS+D+ M    NE++ S FIFIYN P TPVEDE SL Y+++I+DI FMDTD G RC +A LY+GT+E Q+ DCLN+IR+IK+  KPL+ E  W A+V VDV QPP+KKVR
Sbjct:    1 MNTDLIYVSNIPSSMKPVLLLHELENKWSRMLYLENSFSGMRDAFIYNPTPSLPHFHKGVQKLREKVFEDSKYLSVSYVWFPPPNVFITTMIPDGASMLSYLFVENLPKESNVAELTAGLPFQQHDIKLWDIPERQDKCLVIKLPLSSNTSMRSYVLGTLRNRKLKGYFPHVYEIIISDTPELSKVMVPKTFLPTESSESYMANFLFLYNIPSKLNVKSLVDSTKFDNIKGCRVFFIETMNKSKLGFSYIPKTLPYNKFVSLFEKLKSTEYPLDDGTARKIDLNEVWFQPINAEPPKLTEDEVLSENPPEFILTQYLYLHNIPPNYVASQLLMGAPCIVKLYVFSVNDLSTQIGFVVLRGLTPECCQIIIDHFRSLVLGGLPILVDEIYLKCYDLPDISSRDTEHIPRLVLNLPET----KKRCVENSINGDIESNRRLISNIKLSKPPSEGWALMKSDFICLTNLPNALSRSAIIFDIGAVVGGCVLASSSEGKPFAFVYLPDCEELLWEKIVSKLATKTILGNYLDIHEVYLQSSDESMPSGNNEFQSSYFIFIYNLPMTPVEDENSLFYNDVIYDIVFMDTDTGSRCAIAVLYSGTDELQVNDCLNYIRSIKLNKKPLYCERFWLANVHVDVSQPPQKKVR 640
The following BLAST results are available for this feature:
BLAST of SMED30000834 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000834 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000834 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000834 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000834 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000834 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000834 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000834 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000834 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000834 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000834 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000834 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000834 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000834 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000070604.10.000e+097.51SMESG000070604.1[more]
SMESG000070322.10.000e+097.36SMESG000070322.1[more]
SMESG000017548.10.000e+090.98SMESG000017548.1[more]
SMESG000017548.11.833e-2185.38SMESG000017548.1[more]
SMESG000070344.10.000e+073.14SMESG000070344.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30000834 ID=SMED30000834|Name=SMED30000834|organism=Schmidtea mediterranea sexual|type=transcript|length=2097bp
ACTATACATACCACTTTTTTTGTAAACAATGAACACCGATCTTCTATATG
TTTCTAACATTCCAAGTTCAATGAAGCCTGTGCTATTATTGCACGAATTG
GAAAATAAATGGTCGCGAATGTTGTACTTAGAAAATTCTTTCAGTGGAAT
GCGGGACGCTCTGATTTATAATCCTTCATCATCCATGCAACAATTTTACA
ATGGCGTTCAAAAGCTGAGAAGCAAAGTTTTTGATGATAATAAATGTCTA
TCGGTTTCATACGTTTGGTTTCCTCCTCCAAATGTATTTATTACAACAAT
GATTCCTGATGGAGCTTCTATACTTTCGTATATATTTGTGGAGAATTTAC
ATAAGGAAGGTAATGTAGCTGAATTGACAGCTGATTTACCACTTCAACAG
CATGACATTAAGTTATGGGATATTCCAGAAAGACAAGACAAATGCTTGAT
TATTAAATTACCTCCATCCAGCAACACTGCAATGCGAAGCTATGTTTTGG
GTACTTTAAGAAATAGAAAAATTAAAGGATTTTTCCCGCAAGTGTACGAA
ATGATTATTTCAGATACACCAGAACTATCAAAAGTCATGGTTCCGAAAAC
CATTCAAACTACTGAATCATCTGAATCACTTATGGCAAATTTTTTGTTTC
TGCATAACATACCTTCGAAGTTAAATGTTAAAAGTTTAGTGGATTCAACG
AAAATCGACAACATTAAAGGATGTCGAGTGTTCTTTCTTGAAACGATAAA
TAAACTAAAAATGGGATTTATTTATATTCCGAAGACTTTACCATACAATA
AATTTATCGGACTGTTTGAAAAACTAAGAACAAACGAATATACACAGGAT
GACGGTACTAGCTCGAGAATAGATTTAGATGAGGTTTGGTTCCAACCTGC
CGATGCTGATCCACCAAATTTGACAGATGAAGAACAGAGATCGCTCGTTC
CACCGGAATTTGTGTTGATGAACTACTTATACTTGCACAATATTCCACCA
AATTTTGTAGCATCTCAACTGTTGATGGGATCTCCATTTGCAGTGAAGCT
GTATGTGTTCACAGTTAATGATTTGTCTACTCAAATTGGATTTGTAGTGT
TGCGAGGATTGTCTCCGGAAAACTGTCAAATGCTCATTGAGCATTTCCGA
AACCTTGTCATTGGTGGGTTACCGATTTTAGTGGATGAGATTTATTTGAA
ATGCTATAATTTGGCTGACATTGAGACTCGTGATGCAGAATATATTCCTC
GACCTCAATCCACTCGAGTCTATAATAAGAGAAGCATTGACAATTCAACT
AATGGCGCTGTCGAATCAACTAAAAGACGAATCTCTAAAATTAAGCTGTC
CAAATCTCCCCCAGAAGGATGGCCTTTAATGAAATCTGAGTTTATTTGCT
TATCCAATTTACCGAACTCGATGAATAGATCAGCAATTCTATTCAATATC
GGAGTGGCAGTTGGCGGCTGTGTATTGACGACATGTTCTGATGGCAAGCC
GTACGCATTTGTCTATCTGCCCGACTGTGAGCCGACTCTGTGGGAGAATG
TCATCAGCAAATTAGCATCGAAAAACATTCAAGGCAATTTCATGGATATT
CACGAGATCTACCTGCAGTCGAATGACGATTGCATGGCAATTATTGGCAA
TGAGTATCGACCCAGCCCATTTATCTTCATATACAATTTCCCATCTACTC
CCGTTGAGGACGAGCGGTCGTTATTGTACCATGAATTGATATTCGATATT
GCATTCATGGATACCGATAACGGTGGCAGATGCGGTTTAGCTACATTGTA
CAACGGTACTGAAGAGGGACAAATCATTGATTGTCTAAATCACATTCGAA
CAATAAAAATTCAAGGAAAACCTTTATTTGCTGAAATAATGTGGTTTGCT
GATGTCAAGGTCGATGTTGATCAACCTCCCGAGAAAAAAGTCCGCTGCTT
AATGGAATAAAAATGTTATTTATTGTAAATTATGTCTTTGTTTTGATTTG
TTAATATTTCCATTGGATAGTAAATGTATTTAATATCTATTTTTTGCTTG
ATTTTCAAGTTATTTTATTACTTTATCGTATTACAATATTTGCGACG
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protein sequence of SMED30000834-orf-1

>SMED30000834-orf-1 ID=SMED30000834-orf-1|Name=SMED30000834-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=644bp
MNTDLLYVSNIPSSMKPVLLLHELENKWSRMLYLENSFSGMRDALIYNPS
SSMQQFYNGVQKLRSKVFDDNKCLSVSYVWFPPPNVFITTMIPDGASILS
YIFVENLHKEGNVAELTADLPLQQHDIKLWDIPERQDKCLIIKLPPSSNT
AMRSYVLGTLRNRKIKGFFPQVYEMIISDTPELSKVMVPKTIQTTESSES
LMANFLFLHNIPSKLNVKSLVDSTKIDNIKGCRVFFLETINKLKMGFIYI
PKTLPYNKFIGLFEKLRTNEYTQDDGTSSRIDLDEVWFQPADADPPNLTD
EEQRSLVPPEFVLMNYLYLHNIPPNFVASQLLMGSPFAVKLYVFTVNDLS
TQIGFVVLRGLSPENCQMLIEHFRNLVIGGLPILVDEIYLKCYNLADIET
RDAEYIPRPQSTRVYNKRSIDNSTNGAVESTKRRISKIKLSKSPPEGWPL
MKSEFICLSNLPNSMNRSAILFNIGVAVGGCVLTTCSDGKPYAFVYLPDC
EPTLWENVISKLASKNIQGNFMDIHEIYLQSNDDCMAIIGNEYRPSPFIF
IYNFPSTPVEDERSLLYHELIFDIAFMDTDNGGRCGLATLYNGTEEGQII
DCLNHIRTIKIQGKPLFAEIMWFADVKVDVDQPPEKKVRCLME*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000429neoblast
PLANA:0002109X1 cell
PLANA:0002111X2 cell