SMED30000805
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30000805 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 5
Homology
BLAST of SMED30000805 vs. TrEMBL
Match: A0A267EV84 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig001291g2 PE=4 SV=1) HSP 1 Score: 61.2326 bits (147), Expect = 6.017e-6 Identity = 47/164 (28.66%), Postives = 74/164 (45.12%), Query Frame = 2 Query: 1124 LDSEIMSERQRGKILEVALTNSNCKLLDAESNIMELKKRL--------SQKSTDLNKAMESLEVVKRNLEHQSQKSMHLEKTIRSLKLITNERNREYEIRNQATQTVQFMNDLSANKLLEQFEEERKIWLEEKCKVLTYQRQIESNFQQLLIKCRRLETHVLHM 1591 L++E+ ++ +R LE AL SN L+ A + L+++L + ++ D + + LE+T R L L+ ER+RE E F+ ER WLEEK KV+ YQRQ+++N +QL+ CR LE V + Sbjct: 369 LEAELAAQTERASGLEAALAASNESLVAATREVHRLRQQLPTAPAVERACQTEDSDSVESTTAATAAAEAEVEAAQSKLEET-RKLLLLAEERSREQEA---------------------SFQMERSQWLEEKSKVIGYQRQLQANQKQLVATCRHLEGEVARL 510
BLAST of SMED30000805 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006003.1 (pep scaffold:Pmarinus_7.0:GL478646:41833:42718:-1 gene:ENSPMAG00000005428.1 transcript:ENSPMAT00000006003.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 47.7506 bits (112), Expect = 3.674e-6 Identity = 36/79 (45.57%), Postives = 48/79 (60.76%), Query Frame = 2 Query: 1448 KLLEQ---FEEERKIWLEEKCKVLTYQRQIESNFQQLLIKCRRLETHVLHMKSQLKDSRRRPSVSANHSEDHDKSSTNS 1675 KL EQ FEEER++WLEEK KV+ YQRQ +S + Q + R LE V M ++L +SR V A HSE K++ + Sbjct: 162 KLGEQAGAFEEERRVWLEEKDKVIRYQRQQQS-YLQTFQRNRALEEEVRRMAAEL-ESRDMEEVDA-HSEFSYKNAATA 237
BLAST of SMED30000805 vs. Planmine SMEST
Match: SMESG000070031.1 (SMESG000070031.1) HSP 1 Score: 993.416 bits (2567), Expect = 0.000e+0 Identity = 534/540 (98.89%), Postives = 537/540 (99.44%), Query Frame = 2 Query: 128 MRNNYVNSEPNKISTQPYISSLNKSSAFHSTQQSKSSPISKLYVDSTNEKTVSQIQTQNFRQHSFDKLNQSLSIYENFNFTTAMITGDKTLPVRKSRIFDRSIEPSNRKARTEIKRDLSMPPISAKQLTVVENNIYSQYSVEQNHIKYSIDADLDADFNEFDDSPSVMYDSIITTNSITPPPAHIINTSSTSEYVNRKAIIRPLAFRPVQSVNSGTSPTKPPVTLXXXXXXXXXXXXXXXXXHASRMAMKTNLNRVQXXXXXXXXXXXXXXXXXXXXXXXXXXXISPSFDESVSIFDSLLAGEVYSQPAEQPGAIEHFSRESEHDIAGWKLSLDSEIMSERQRGKILEVALTNSNCKLLDAESNIMELKKRLSQKSTDLNKAMESLEVVKRNLEHQSQKSMHLEKTIRSLKLITNERNREYEIRNQATQTVQFMNDLSANKLLEQFEEERKIWLEEKCKVLTYQRQIESNFQQLLIKCRRLETHVLHMKSQLKDSRRRPSVSANHSEDHDKSSTNSVDSSNRNYFTNVSRIEVTEHQSSF 1747 MRNNYVNSEPNKISTQPYISSLNKSSAFHSTQQSKSS ISKLYVDSTNEKTVSQIQTQNFRQHSFDKLNQSLSIYENFNFTTAMITGDKTLPVRKSRIFDRSIEPSNRKARTEIKRDLSMPPISAKQLTVVENNIYSQYSVEQNHIKYSIDADLDADFNEFDDSPSVMYDSIITTNSITPPP HIINTSSTSEYVNRKAIIRPLAFRPVQSVNSGTSPTKPPVTLKPKIPEPKKSSPPPVPPHA+RMAMKTNLNRVQSAKSGDSSGYYSSRTTGTLTGTASSTDISPSFDESVSIFDSLLAGEVYSQPAEQPGAIEHF+RESEHDIAGWKLSLDSEIMSERQRGKILEVALTNSNCKLLDAESNIMELKKRLSQKSTD NKAMESLEVVKRNLEHQSQKSMHLEKTI+SLKLITNERNREYEIRNQATQTVQFMNDLSANKLLEQFEEERKIWLEEKCKVLTYQRQIESNFQQLLIKCRRLETHVLHMKSQLKDSRRRPSVSANHSEDHDKSSTNSVDSSNRNYFTNVSRIEVTEHQSSF Sbjct: 1 MRNNYVNSEPNKISTQPYISSLNKSSAFHSTQQSKSSAISKLYVDSTNEKTVSQIQTQNFRQHSFDKLNQSLSIYENFNFTTAMITGDKTLPVRKSRIFDRSIEPSNRKARTEIKRDLSMPPISAKQLTVVENNIYSQYSVEQNHIKYSIDADLDADFNEFDDSPSVMYDSIITTNSITPPPVHIINTSSTSEYVNRKAIIRPLAFRPVQSVNSGTSPTKPPVTLKPKIPEPKKSSPPPVPPHATRMAMKTNLNRVQSAKSGDSSGYYSSRTTGTLTGTASSTDISPSFDESVSIFDSLLAGEVYSQPAEQPGAIEHFNRESEHDIAGWKLSLDSEIMSERQRGKILEVALTNSNCKLLDAESNIMELKKRLSQKSTDFNKAMESLEVVKRNLEHQSQKSMHLEKTIQSLKLITNERNREYEIRNQATQTVQFMNDLSANKLLEQFEEERKIWLEEKCKVLTYQRQIESNFQQLLIKCRRLETHVLHMKSQLKDSRRRPSVSANHSEDHDKSSTNSVDSSNRNYFTNVSRIEVTEHQSSF 540
BLAST of SMED30000805 vs. Planmine SMEST
Match: SMESG000070031.1 (SMESG000070031.1) HSP 1 Score: 822.002 bits (2122), Expect = 0.000e+0 Identity = 451/457 (98.69%), Postives = 454/457 (99.34%), Query Frame = 2 Query: 128 MRNNYVNSEPNKISTQPYISSLNKSSAFHSTQQSKSSPISKLYVDSTNEKTVSQIQTQNFRQHSFDKLNQSLSIYENFNFTTAMITGDKTLPVRKSRIFDRSIEPSNRKARTEIKRDLSMPPISAKQLTVVENNIYSQYSVEQNHIKYSIDADLDADFNEFDDSPSVMYDSIITTNSITPPPAHIINTSSTSEYVNRKAIIRPLAFRPVQSVNSGTSPTKPPVTLXXXXXXXXXXXXXXXXXHASRMAMKTNLNRVQXXXXXXXXXXXXXXXXXXXXXXXXXXXISPSFDESVSIFDSLLAGEVYSQPAEQPGAIEHFSRESEHDIAGWKLSLDSEIMSERQRGKILEVALTNSNCKLLDAESNIMELKKRLSQKSTDLNKAMESLEVVKRNLEHQSQKSMHLEKTIRSLKLITNERNREYEIRNQATQTVQFMNDLSANKLLEQFEEERKIWLEEK 1498 MRNNYVNSEPNKISTQPYISSLNKSSAFHSTQQSKSS ISKLYVDSTNEKTVSQIQTQNFRQHSFDKLNQSLSIYENFNFTTAMITGDKTLPVRKSRIFDRSIEPSNRKARTEIKRDLSMPPISAKQLTVVENNIYSQYSVEQNHIKYSIDADLDADFNEFDDSPSVMYDSIITTNSITPPP HIINTSSTSEYVNRKAIIRPLAFRPVQSVNSGTSPTKPPVTLKPKIPEPKKSSPPPVPPHA+RMAMKTNLNRVQSAKSGDSSGYYSSRTTGTLTGTASSTDISPSFDESVSIFDSLLAGEVYSQPAEQPGAIEHF+RESEHDIAGWKLSLDSEIMSERQRGKILEVALTNSNCKLLDAESNIMELKKRLSQKSTD NKAMESLEVVKRNLEHQSQKSMHLEKTI+SLKLITNERNREYEIRNQATQTVQFMNDLSANKLLEQFEEERKIWLEEK Sbjct: 1 MRNNYVNSEPNKISTQPYISSLNKSSAFHSTQQSKSSAISKLYVDSTNEKTVSQIQTQNFRQHSFDKLNQSLSIYENFNFTTAMITGDKTLPVRKSRIFDRSIEPSNRKARTEIKRDLSMPPISAKQLTVVENNIYSQYSVEQNHIKYSIDADLDADFNEFDDSPSVMYDSIITTNSITPPPVHIINTSSTSEYVNRKAIIRPLAFRPVQSVNSGTSPTKPPVTLKPKIPEPKKSSPPPVPPHATRMAMKTNLNRVQSAKSGDSSGYYSSRTTGTLTGTASSTDISPSFDESVSIFDSLLAGEVYSQPAEQPGAIEHFNRESEHDIAGWKLSLDSEIMSERQRGKILEVALTNSNCKLLDAESNIMELKKRLSQKSTDFNKAMESLEVVKRNLEHQSQKSMHLEKTIQSLKLITNERNREYEIRNQATQTVQFMNDLSANKLLEQFEEERKIWLEEK 457
BLAST of SMED30000805 vs. Planmine SMEST
Match: SMESG000042161.1 (SMESG000042161.1) HSP 1 Score: 52.7582 bits (125), Expect = 6.545e-7 Identity = 24/51 (47.06%), Postives = 36/51 (70.59%), Query Frame = 2 Query: 1451 LLEQFEEERKIWLEEKCKVLTYQRQIESNFQQLLIKCRRLETHVLHMKSQL 1603 L +QFEEER WLEEK +V++Y +Q+ +Q LL K +LE +L+++ QL Sbjct: 320 LQQQFEEERAKWLEEKAQVISYHKQLNLKYQNLLEKNVKLENELLNLQQQL 370
BLAST of SMED30000805 vs. Planmine SMEST
Match: SMESG000042161.1 (SMESG000042161.1) HSP 1 Score: 52.7582 bits (125), Expect = 7.323e-7 Identity = 24/51 (47.06%), Postives = 36/51 (70.59%), Query Frame = 2 Query: 1451 LLEQFEEERKIWLEEKCKVLTYQRQIESNFQQLLIKCRRLETHVLHMKSQL 1603 L +QFEEER WLEEK +V++Y +Q+ +Q LL K +LE +L+++ QL Sbjct: 312 LQQQFEEERAKWLEEKAQVISYHKQLNLKYQNLLEKNVKLENELLNLQQQL 362 The following BLAST results are available for this feature:
BLAST of SMED30000805 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30000805 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30000805 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30000805 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30000805 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30000805 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30000805 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30000805 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 1
BLAST of SMED30000805 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30000805 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 1
BLAST of SMED30000805 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30000805 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30000805 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30000805 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 4
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30000805 ID=SMED30000805|Name=SMED30000805|organism=Schmidtea mediterranea sexual|type=transcript|length=2215bpback to top protein sequence of SMED30000805-orf-1 >SMED30000805-orf-1 ID=SMED30000805-orf-1|Name=SMED30000805-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=541bp MRNNYVNSEPNKISTQPYISSLNKSSAFHSTQQSKSSPISKLYVDSTNEKback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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