cytochrome b

Overview
Namecytochrome b (YP_008592437.2)
Smed IDSMED30000702
Length (bp)1101
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of cytochrome b (SMED30000702) t-SNE clustered cells

Violin plots show distribution of expression levels for cytochrome b (SMED30000702) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of cytochrome b (SMED30000702) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for cytochrome b (SMED30000702) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30000702

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 17

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30000702 dd_Smed_v4_344_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30000702 dd_Smed_v4_344_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30000702 dd_Smed_v4_344_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30000702 dd_Smed_v4_344_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30000702 dd_Smed_v4_344_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30000702 dd_Smed_v4_344_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30000702 dd_Smed_v4_344_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30000702 dd_Smed_v4_344_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30000702 dd_Smed_v4_344_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30000702 dd_Smed_v4_344_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30000702 dd_Smed_v4_344_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30000702 Contig44099newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30000702 Contig44099uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30000702 Contig38381uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30000702 Contig38381newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
gamma neoblastSMED30000702 dd_Smed_v6_344_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30000702 dd_Smed_v6_344_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of cytochrome b vs. Ensembl Human
Match: MT-CYB (mitochondrially encoded cytochrome b [Source:HGNC Symbol;Acc:HGNC:7427])

HSP 1 Score: 228.024 bits (580), Expect = 6.126e-71
Identity = 149/327 (45.57%), Postives = 204/327 (62.39%), Query Frame = 1
Query:   19 VYLPTPKKIDYFWCXXXXXXXXXXXXXXXXXXXXXXYLPSVYFSFDSVDCFYREVLFGSFFRRVHSIGASXXXXXXXXXXXRGLYFSSYSYVSTWLVGCLILVLSMAIAFLGYVLP*GNMSLWGATVITSLLSAIPYFGSSLVEWL*GGFSISSPTLSRFFVLHFFLPFILAIFVFSHILFLHVSGSGNPLGINSKGRSLMFTPYFLINDLLGXXXXXXXXXXXXXXXXXXXXDSSNFDEANFLSTPLHIKPEWYFLAAYAVLRCVPNKLGGVXXXXXXXXXXXXX----XXXVSSVYIVPLDFFGSVFYFMFICNFVLLTLLGGLP 987
            + LPTP  I  +W FGSLL    ++Q  +G FL+  Y P    +F S+    R+V +G   R +H+ GAS FF+ ++ H+ RGLY+ S+ Y  TW +G ++L+ +MA AF+GYVLP G MS WGATVIT+LLSAIPY G+ LV+W+ GG+S+ SPTL+RFF  HF LPFI+A     H+LFLH +GS NPLGI S    + F PY+ I D LG ++  +  + L ++ P    D  N+  AN L+TP HIKPEWYFL AY +LR VPNKLGGV  L + + I  +I         S+   PL       Y++   + ++LT +GG P
Sbjct:   19 IDLPTPSNISAWWNFGSLLGACLILQITTGLFLAMHYSPDASTAFSSIAHITRDVNYGWIIRYLHANGASMFFICLFLHIGRGLYYGSFLYSETWNIGIILLLATMATAFMGYVLPWGQMSFWGATVITNLLSAIPYIGTDLVQWIWGGYSVDSPTLTRFFTFHFILPFIIAALATLHLLFLHETGSNNPLGITSHSDKITFHPYYTIKDALGLLLFLLSLMTLTLFSPDLLGDPDNYTLANPLNTPPHIKPEWYFLFAYTILRSVPNKLGGVLALLLSILILAMIPILHMSKQQSMMFRPLS---QSLYWLLAADLLILTWIGGQP 342          
BLAST of cytochrome b vs. Ensembl Celegans
Match: ctb-1 (Cytochrome b [Source:UniProtKB/Swiss-Prot;Acc:P24890])

HSP 1 Score: 158.303 bits (399), Expect = 8.253e-45
Identity = 104/237 (43.88%), Postives = 145/237 (61.18%), Query Frame = 1
Query:  127 YLPSVYFSFDSVDCFYREVLFGSFFRRVHSIGASXXXXXXXXXXXRGLYFSSYSYVSTWLVGCLILVLSMAIAFLGYVLP*GNMSLWGATVITSLLSAIPYFGSSLVEWL*GGFSISSPTLSRFFVLHFFLPFILAIFVFSHILFLHVSGSGNPLGINSKGRSLMFTPYFLINDLLGXXXXXXXXXXXXXXXXXXXXDSSNFDEANFLSTPLHIKPEWYFLAAYAVLRCVPNKLGGV 837
            Y P    +F +V     EV FG  FR  H  GAS FF+F+Y H+F+GL+F SY     W+ G  I +L M  AF+GYVL    MS W A VITSLLS IP +G ++V W+  GF ++  TL  FFVLHF LP+ + + V  H++FLH +GS + L  +     + F+P +L  D    +V +++F+ L +  PF   D+  F EA+ + +P+HI PEWYFL AYA+LR +PNK+ GV
Sbjct:   52 YTPDSLMAFSTVQYIMYEVNFGWVFRIFHFNGASLFFIFLYLHIFKGLFFMSYRLKKVWMSGLTIYLLVMMEAFMGYVLVWAQMSFWAAVVITSLLSVIPIWGPTIVTWIWSGFGVTGATLKFFFVLHFLLPWAILVIVLGHLIFLHSTGSTSSLYCHGDYDKVCFSPEYLGKDAYN-IVIWLLFIVLSLIYPFNLGDAEMFIEADPMMSPVHIVPEWYFLFAYAILRAIPNKVLGV 287          
BLAST of cytochrome b vs. Ensembl Fly
Match: mt:Cyt-b (gene:FBgn0013678 transcript:FBtr0433502)

HSP 1 Score: 223.787 bits (569), Expect = 1.103e-69
Identity = 140/273 (51.28%), Postives = 183/273 (67.03%), Query Frame = 1
Query:   19 VYLPTPKKIDYFWCXXXXXXXXXXXXXXXXXXXXXXYLPSVYFSFDSVDCFYREVLFGSFFRRVHSIGASXXXXXXXXXXXRGLYFSSYSYVSTWLVGCLILVLSMAIAFLGYVLP*GNMSLWGATVITSLLSAIPYFGSSLVEWL*GGFSISSPTLSRFFVLHFFLPFILAIFVFSHILFLHVSGSGNPLGINSKGRSLMFTPYFLINDLLGXXXXXXXXXXXXXXXXXXXXDSSNFDEANFLSTPLHIKPEWYFLAAYAVLRCVPNKLGGV 837
            V LP P  I  +W FGSLL +  ++Q ++G FL+  Y   +  +F SV+   R+V +G   R +H+ GASFFF+ IY H+ RG+Y+ SY +  TWL+G +IL L M  AF+GYVLP G MS WGATVIT+LLSAIPY G  LV+WL GGF++ + TL+RFF  HF LPFI+      H+LFLH +GS NP+G+NS    + F PYF   D++GF+V   + + LV+  P    D  NF  AN L TP HI+PEWYFL AYA+LR +PNKLGGV
Sbjct:   20 VDLPAPINISSWWNFGSLLGLCLIIQILTGLFLAMHYTADINLAFYSVNHICRDVNYGWLLRTLHANGASFFFICIYLHVGRGIYYGSYKFTPTWLIGVIILFLVMGTAFMGYVLPWGQMSFWGATVITNLLSAIPYLGMDLVQWLWGGFAVDNATLTRFFTFHFILPFIVLAMTMIHLLFLHQTGSNNPIGLNSNIDKIPFHPYFTFKDIVGFIVMIFILISLVLISPNLLGDPDNFIPANPLVTPAHIQPEWYFLFAYAILRSIPNKLGGV 292          
BLAST of cytochrome b vs. Ensembl Zebrafish
Match: mt-cyb (cytochrome b [Source:NCBI gene;Acc:140512])

HSP 1 Score: 224.172 bits (570), Expect = 8.955e-70
Identity = 142/273 (52.01%), Postives = 180/273 (65.93%), Query Frame = 1
Query:   19 VYLPTPKKIDYFWCXXXXXXXXXXXXXXXXXXXXXXYLPSVYFSFDSVDCFYREVLFGSFFRRVHSIGASXXXXXXXXXXXRGLYFSSYSYVSTWLVGCLILVLSMAIAFLGYVLP*GNMSLWGATVITSLLSAIPYFGSSLVEWL*GGFSISSPTLSRFFVLHFFLPFILAIFVFSHILFLHVSGSGNPLGINSKGRSLMFTPYFLINDLLGXXXXXXXXXXXXXXXXXXXXDSSNFDEANFLSTPLHIKPEWYFLAAYAVLRCVPNKLGGV 837
            V LPTP  I  +W FGSLL +  + Q ++G FL+  Y   +  +F SV    R+V FG   R +H+ GASFFF+ +Y H+ RGLY+ SY Y  TW +G ++ +L M  AF+GYVLP G MS WGATVIT+LLSA+PY G +LV+W+ GGFS+ + TL+RFF  HF LPFI+   V  H+LFLH +GS NPLG+N     + F PYF   DLLGFV+       L ++ P    D  NF  AN L TP HIKPEWYFL AYA+LR +PNKLGGV
Sbjct:   19 VDLPTPLNISAWWNFGSLLGLCLITQILTGLFLAMHYTSDISTAFSSVVHICRDVNFGWLIRSIHANGASFFFICLYIHIARGLYYGSYLYNETWNIGVVLFLLVMMTAFVGYVLPWGQMSFWGATVITNLLSAVPYVGDTLVQWIWGGFSVDNATLTRFFAFHFLLPFIIIAMVILHLLFLHETGSNNPLGLNPNMDKIPFHPYFSNKDLLGFVIMLFSLSLLALFSPNLLGDPENFTPANPLVTPPHIKPEWYFLFAYAILRSIPNKLGGV 291          
BLAST of cytochrome b vs. Ensembl Xenopus
Match: trim33 (tripartite motif containing 33 [Source:Xenbase;Acc:XB-GENE-1216165])

HSP 1 Score: 222.246 bits (565), Expect = 8.802e-69
Identity = 141/271 (52.03%), Postives = 178/271 (65.68%), Query Frame = 1
Query:   25 LPTPKKIDYFWCXXXXXXXXXXXXXXXXXXXXXXYLPSVYFSFDSVDCFYREVLFGSFFRRVHSIGASXXXXXXXXXXXRGLYFSSYSYVSTWLVGCLILVLSMAIAFLGYVLP*GNMSLWGATVITSLLSAIPYFGSSLVEWL*GGFSISSPTLSRFFVLHFFLPFILAIFVFSHILFLHVSGSGNPLGINSKGRSLMFTPYFLINDLLGXXXXXXXXXXXXXXXXXXXXDSSNFDEANFLSTPLHIKPEWYFLAAYAVLRCVPNKLGGV 837
            LPTP  +  +W FGSLL V  + Q  +G FL+  Y      +F SV    R+V +G   R +H+ GASFFF+ IY H+ RGLY+ S+ Y  TW +G ++L L MA AF+GYVLP G MS WGATVIT+LLSAIPY G+ LV+W+ GGFS+ + TL+RFF  HF LPF++A     H+LFLH +GS NP G+NS    + F PYF   DLLGF++       L ++ P    D  NF  AN L TP HIKPEWYFL AYA+LR +PNKLGGV
Sbjct:   22 LPTPSNLSSWWNFGSLLGVCLIAQIATGLFLAMHYTADTSMAFSSVAHICRDVNYGWLIRNLHANGASFFFICIYLHIGRGLYYGSFLYKETWNIGVILLFLVMATAFVGYVLPWGQMSFWGATVITNLLSAIPYIGNVLVQWIWGGFSVDNATLTRFFAFHFLLPFLIAGASILHLLFLHETGSTNPTGLNSDPDKIPFHPYFSYKDLLGFLIMLTSLTLLAMFAPNLLGDPENFTPANPLVTPPHIKPEWYFLFAYAILRSIPNKLGGV 292          
BLAST of cytochrome b vs. Ensembl Mouse
Match: mt-Cytb (mitochondrially encoded cytochrome b [Source:MGI Symbol;Acc:MGI:102501])

HSP 1 Score: 233.417 bits (594), Expect = 2.655e-73
Identity = 152/325 (46.77%), Postives = 209/325 (64.31%), Query Frame = 1
Query:   25 LPTPKKIDYFWCXXXXXXXXXXXXXXXXXXXXXXYLPSVYFSFDSVDCFYREVLFGSFFRRVHSIGASXXXXXXXXXXXRGLYFSSYSYVSTWLVGCLILVLSMAIAFLGYVLP*GNMSLWGATVITSLLSAIPYFGSSLVEWL*GGFSISSPTLSRFFVLHFFLPFILAIFVFSHILFLHVSGSGNPLGINSKGRSLMFTPYFLINDLLGXXXXXXXXXXXXXXXXXXXXDSSNFDEANFLSTPLHIKPEWYFLAAYAVLRCVPNKLGGVXXXXXXXXXXXXX----XXXVSSVYIVPLDFFGSVFYFMFICNFVLLTLLGGLP 987
            LP P  I  +W FGSLL V  +VQ ++G FL+  Y      +F SV    R+V +G   R +H+ GAS FF+ ++ H+ RGLY+ SY+++ TW +G L+L   MA AF+GYVLP G MS WGATVIT+LLSAIPY G++LVEW+ GGFS+   TL+RFF  HF LPFI+A     H+LFLH +GS NP G+NS    + F PY+ I D+LG ++ F++ + LV++ P    D  N+  AN L+TP HIKPEWYFL AYA+LR +PNKLGGV  L + + I  L+         S+   P+     + Y++ + N ++LT +GG P
Sbjct:   21 LPAPSNISSWWNFGSLLGVCLMVQIITGLFLAMHYTSDTMTAFSSVTHICRDVNYGWLIRYMHANGASMFFICLFLHVGRGLYYGSYTFMETWNIGVLLLFAVMATAFMGYVLPWGQMSFWGATVITNLLSAIPYIGTTLVEWIWGGFSVDKATLTRFFAFHFILPFIIAALAIVHLLFLHETGSNNPTGLNSDADKIPFHPYYTIKDILGILIMFLILMTLVLFFPDMLGDPDNYMPANPLNTPPHIKPEWYFLFAYAILRSIPNKLGGVLALILSILILALMPFLHTSKQRSLMFRPIT---QILYWILVANLLILTWIGGQP 342          
BLAST of cytochrome b vs. UniProt/SwissProt
Match: sp|Q9BA02|CYB_SIGGR (Cytochrome b OS=Sigmops gracilis OX=48457 GN=mt-cyb PE=3 SV=1)

HSP 1 Score: 239.58 bits (610), Expect = 9.307e-75
Identity = 152/324 (46.91%), Postives = 201/324 (62.04%), Query Frame = 1
Query:   19 VYLPTPKKIDYFWCXXXXXXXXXXXXXXXXXXXXXXYLPSVYFSFDSVDCFYREVLFGSFFRRVHSIGASXXXXXXXXXXXRGLYFSSYSYVSTWLVGCLILVLSMAIAFLGYVLP*GNMSLWGATVITSLLSAIPYFGSSLVEWL*GGFSISSPTLSRFFVLHFFLPFILAIFVFSHILFLHVSGSGNPLGINSKGRSLMFTPYFLINDLLGXXXXXXXXXXXXXXXXXXXXDSSNFDEANFLSTPLHIKPEWYFLAAYAVLRCVPNKLGGVXXXXXXXXXXXXX-XXXVSSVYIVPLDFFGSVFYFMFICNFVLLTLLGGLP 987
            + LPTP  I  +W FGSLL +    Q V+G FL+  Y   +  +F SV    R+V +G   R +H+ GASFFF+ +Y H+ RGLY+ SY Y  TW VG ++L+L+M  AF+GYVLP G MS WGATVIT+LLSAIPY G +LV+W+ GGFS+ + TL+RFF  HF LPF++A F   H+LFLH +GS NP G+NS    + F PYF + DLLGF +  +    + +  P    D  NF  AN L TP HIKPEWYFL AYA+LR +PNKLGGV  L   + I   +     S    +       + ++  I N  +LT +GG+P
Sbjct:   19 IDLPTPSNISAWWNFGSLLGLCLASQIVTGLFLAMHYTSDISTAFSSVTHICRDVNYGWLVRNMHANGASFFFICLYTHIGRGLYYGSYLYKETWTVGVILLLLTMMTAFVGYVLPWGQMSFWGATVITNLLSAIPYAGQTLVQWIWGGFSVDNATLTRFFAFHFLLPFVIAAFTAIHLLFLHETGSNNPTGLNSDADKIPFHPYFSLKDLLGFTILILTLTSVALLTPNLLGDPDNFTPANPLVTPPHIKPEWYFLFAYAILRSIPNKLGGVLALLASVLILATVPFLQTSKQQALTFRPLTQLVFWTLIANIAILTWIGGMP 342          
BLAST of cytochrome b vs. UniProt/SwissProt
Match: sp|Q7YC74|CYB_RHYIS (Cytochrome b OS=Rhynchomys isarogensis OX=238000 GN=MT-CYB PE=3 SV=1)

HSP 1 Score: 237.654 bits (605), Expect = 5.006e-74
Identity = 151/327 (46.18%), Postives = 206/327 (63.00%), Query Frame = 1
Query:   19 VYLPTPKKIDYFWCXXXXXXXXXXXXXXXXXXXXXXYLPSVYFSFDSVDCFYREVLFGSFFRRVHSIGASXXXXXXXXXXXRGLYFSSYSYVSTWLVGCLILVLSMAIAFLGYVLP*GNMSLWGATVITSLLSAIPYFGSSLVEWL*GGFSISSPTLSRFFVLHFFLPFILAIFVFSHILFLHVSGSGNPLGINSKGRSLMFTPYFLINDLLGXXXXXXXXXXXXXXXXXXXXDSSNFDEANFLSTPLHIKPEWYFLAAYAVLRCVPNKLGGVXXXXXXXXXXXXX----XXXVSSVYIVPLDFFGSVFYFMFICNFVLLTLLGGLP 987
            + LPTP  I  +W FGSLL +   +Q ++G FL+  Y      +F SV    R+V +G   R +H+ GAS FF+ ++ H+ RGLY+ SY+ + TW +G L+L   MA AF+GYVLP G MS WGATVIT+LLSAIPY G++LVEW+ GGFS+   TL+RFF  HF LPFI+   V  H+LFLH +GS NP G+NS    + F PY+ I DLLG ++  +  + LV++ P    D  N+  AN L+TP HIKPEWYFL AYA+LR +PNKLGGV  L + + +  L+         S+   P+       Y+M + N ++LT +GG P
Sbjct:   19 IDLPTPSNISSWWNFGSLLGLCLAIQIITGLFLAMHYTADTTTAFSSVAHICRDVNYGWLIRYLHANGASMFFICLFLHVGRGLYYGSYTLMETWNIGVLLLFTVMATAFMGYVLPWGQMSFWGATVITNLLSAIPYIGTTLVEWIWGGFSVDKATLTRFFAFHFILPFIITALVIVHLLFLHETGSNNPTGLNSNADKIPFHPYYTIKDLLGVILLILSTMSLVLFFPDLLSDPDNYTPANPLNTPPHIKPEWYFLFAYAILRSIPNKLGGVLALILSILVLALLPFLHTSKQRSLMFRPIT---QTLYWMLVANLLVLTWIGGQP 342          
BLAST of cytochrome b vs. UniProt/SwissProt
Match: sp|Q8LWN0|CYB_TAMTE (Cytochrome b OS=Tamandua tetradactyla OX=48850 GN=MT-CYB PE=3 SV=1)

HSP 1 Score: 237.269 bits (604), Expect = 5.556e-74
Identity = 151/327 (46.18%), Postives = 207/327 (63.30%), Query Frame = 1
Query:   19 VYLPTPKKIDYFWCXXXXXXXXXXXXXXXXXXXXXXYLPSVYFSFDSVDCFYREVLFGSFFRRVHSIGASXXXXXXXXXXXRGLYFSSYSYVSTWLVGCLILVLSMAIAFLGYVLP*GNMSLWGATVITSLLSAIPYFGSSLVEWL*GGFSISSPTLSRFFVLHFFLPFILAIFVFSHILFLHVSGSGNPLGINSKGRSLMFTPYFLINDLLGXXXXXXXXXXXXXXXXXXXXDSSNFDEANFLSTPLHIKPEWYFLAAYAVLRCVPNKLGGVXXXXXXXXXXXXX----XXXVSSVYIVPLDFFGSVFYFMFICNFVLLTLLGGLP 987
            + LPTP  I  +W FGSLL V  V+Q ++G FL+  Y      +F SV    R+V +G   R +H+ GAS FF+ +Y H+ RGLY+ SY Y+ TW +G ++L+ +MA AF+GYVLP G MS WGATVIT+LLSAIPY G+ LVEW+ GGFS+   TL+RFF  HF LPFI+     +H+LFLH +GS NPLG++S    + F PY+ I D+LG ++     + LV++ P    D  N+  AN LSTP HIKPEWYFL AYA+LR +PNKLGGV  L   + +  ++         S+   PL     + + + + N ++LT +GG P
Sbjct:   19 IDLPTPSNISAWWNFGSLLGVCLVIQTLTGLFLAMHYTSDTITAFSSVTHICRDVNYGWLIRYIHANGASMFFMCLYLHVGRGLYYGSYLYLETWNIGVILLLATMATAFMGYVLPWGQMSFWGATVITNLLSAIPYIGTDLVEWIWGGFSVDKATLTRFFAFHFILPFIITALAMTHLLFLHETGSNNPLGLSSDMDKIPFHPYYTIKDILGLLIMIATLVTLVLFSPDLLGDPDNYTPANPLSTPPHIKPEWYFLFAYAILRSIPNKLGGVVALVCSILVLMIMPLLHTAKQRSMMFRPLS---QLLFRLLVANLLILTWIGGQP 342          
BLAST of cytochrome b vs. UniProt/SwissProt
Match: sp|Q8SJK5|CYB_GLYMU (Cytochrome b OS=Glyptemys muhlenbergii OX=335393 GN=MT-CYB PE=3 SV=1)

HSP 1 Score: 236.498 bits (602), Expect = 1.094e-73
Identity = 152/324 (46.91%), Postives = 203/324 (62.65%), Query Frame = 1
Query:   19 VYLPTPKKIDYFWCXXXXXXXXXXXXXXXXXXXXXXYLPSVYFSFDSVDCFYREVLFGSFFRRVHSIGASXXXXXXXXXXXRGLYFSSYSYVSTWLVGCLILVLSMAIAFLGYVLP*GNMSLWGATVITSLLSAIPYFGSSLVEWL*GGFSISSPTLSRFFVLHFFLPFILAIFVFSHILFLHVSGSGNPLGINSKGRSLMFTPYFLINDLLGXXXXXXXXXXXXXXXXXXXXDSSNFDEANFLSTPLHIKPEWYFLAAYAVLRCVPNKLGGVXXXXXXXXXXXXX-XXXVSSVYIVPLDFFGSVFYFMFICNFVLLTLLGGLP 987
            + LP+P  I  +W FGSLL    ++Q ++G FL+  Y P +  +F SV    R+V +G   R +H+ GAS FF+ IY H+ RGLY+ SY Y  TW  G  +L+L+MA AF+GYVLP G MS WGATVIT+LLSA+PY G++LV+W+ GGFS+ + TL+RFF  HF LPF +      H+LFLH +GS NP G+NS    + F PYF   DLLG ++     L L ++ P    D  NF  AN LSTP HIKPEWYFL AYA+LR +PNKLGGV  L + + + FL+     S    +         ++ FI N ++LT +GG P
Sbjct:   20 IDLPSPSNISAWWNFGSLLGACLILQTITGIFLAMHYSPDISLAFSSVAHITRDVQYGWLIRNMHANGASLFFMCIYLHIGRGLYYGSYLYKETWNTGITLLLLTMATAFVGYVLPWGQMSFWGATVITNLLSAVPYIGNTLVQWIWGGFSVDNATLTRFFTFHFLLPFTIMGMTMVHLLFLHETGSNNPTGLNSNTDKIPFHPYFSYKDLLGLILMLAFLLTLTLFYPNLLGDPDNFTPANPLSTPPHIKPEWYFLFAYAILRSIPNKLGGVLALLLSILVLFLMPTLHTSKQRTIQFRPLTQTLFWSFIANLMVLTWIGGQP 343          
BLAST of cytochrome b vs. UniProt/SwissProt
Match: sp|Q7YC63|CYB_APOMU (Cytochrome b OS=Apomys musculus OX=238007 GN=MT-CYB PE=3 SV=1)

HSP 1 Score: 236.113 bits (601), Expect = 1.600e-73
Identity = 151/325 (46.46%), Postives = 206/325 (63.38%), Query Frame = 1
Query:   25 LPTPKKIDYFWCXXXXXXXXXXXXXXXXXXXXXXYLPSVYFSFDSVDCFYREVLFGSFFRRVHSIGASXXXXXXXXXXXRGLYFSSYSYVSTWLVGCLILVLSMAIAFLGYVLP*GNMSLWGATVITSLLSAIPYFGSSLVEWL*GGFSISSPTLSRFFVLHFFLPFILAIFVFSHILFLHVSGSGNPLGINSKGRSLMFTPYFLINDLLGXXXXXXXXXXXXXXXXXXXXDSSNFDEANFLSTPLHIKPEWYFLAAYAVLRCVPNKLGGVXXXXXXXXXXX----XXXXXVSSVYIVPLDFFGSVFYFMFICNFVLLTLLGGLP 987
            LP P  I  +W FGSLL +  ++Q ++G FL+  Y      +F SV    R+V +G   R +H+ GAS FF+ ++ H+ RG+Y+ SY+++ TW +G ++L   MA AF+GYVLP G MS WGATVIT+LLSAIPY G++LVEW+ GGFS+   TL+RFF  HF LPFI+A  V  H+LFLH +GS NP G+NS    + F PY+ I DLLG  +  +  + LV++ P    D  N+  AN L+TP HIKPEWYFL AYA+LR +PNKLGGV  L + + I      L      S+   P+     V Y+M + N ++LT +GG P
Sbjct:   21 LPAPSNISSWWNFGSLLGLCLMIQIITGLFLAMHYTSDTTTAFSSVTHICRDVNYGWLIRYMHANGASMFFICLFIHIGRGMYYGSYTFMETWNIGVILLFAIMATAFMGYVLPWGQMSFWGATVITNLLSAIPYIGTTLVEWIWGGFSVDKATLTRFFAFHFILPFIIAALVIVHLLFLHETGSNNPTGLNSNADKIPFHPYYTIKDLLGVFMLILFLMTLVLFFPDMLGDPDNYTPANPLNTPPHIKPEWYFLFAYAILRSIPNKLGGVLALILSILILAFLPFLHTSKQRSLMFRPIT---QVLYWMLVANLLILTWIGGQP 342          
BLAST of cytochrome b vs. TrEMBL
Match: T1PTK7 (Cytochrome b (Fragment) OS=Schmidtea mediterranea OX=79327 GN=CytB PE=3 SV=2)

HSP 1 Score: 498.049 bits (1281), Expect = 5.166e-174
Identity = 358/364 (98.35%), Postives = 358/364 (98.35%), Query Frame = 1
Query:    7 LLSNVYLPTPKKIDYFWCXXXXXXXXXXXXXXXXXXXXXXYLPSVYFSFDSVDCFYREVLFGSFFRRVHSIGASXXXXXXXXXXXRGLYFSSYSYVSTWLVGCLILVLSMAIAFLGYVLP*GNMSLWGATVITSLLSAIPYFGSSLVEWL*GGFSISSPTLSRFFVLHFFLPFILAIFVFSHILFLHVSGSGNPLGINSKGRSLMFTPYFLINDLLGXXXXXXXXXXXXXXXXXXXXDSSNFDEANFLSTPLHIKPEWYFLAAYAVLRCVPNKLGGVXXXXXXXXXXXXXXXXVSSVYIVPLDFFGSVFYFMFICNFVLLTLLGGLPXXXXXXXXXXXXXXXXXIYFVLLLDISWLDYVSMIRL 1098
            LLSNVYLPTPK IDYFWCFGSLLLV FVVQFVSGFFLSSFYLPSVYFSFDSVDCFYREVLFGSFFR VHSIGASFFFLFIYFHLFRGLYFSSYSYVSTWLVGCLILVLSMAIAFLGYVLP GNMSLWGATVITSLLSAIPYFGSSLVEWL GGFSISSPTLSRFFVLHFFLPFILAIFVFSHILFLHVSGSGNPLGINSKGRSLMFTPYFLINDLLGFVVCFIVFLYLVVYVPFFQFDSSNFDEANFLSTPLHIKPEWYFLAAYAVLRCVPNKLGGVYFLFMFLFIYFLIPFLVSSVYIVPLDFFGSVFYFMFICNFVLLTLLGGLPVESPFV VSVFSSFLYFIYFVLLLDISWLDYVSMIRL
Sbjct:    1 LLSNVYLPTPKNIDYFWCFGSLLLVWFVVQFVSGFFLSSFYLPSVYFSFDSVDCFYREVLFGSFFRSVHSIGASFFFLFIYFHLFRGLYFSSYSYVSTWLVGCLILVLSMAIAFLGYVLPWGNMSLWGATVITSLLSAIPYFGSSLVEWLWGGFSISSPTLSRFFVLHFFLPFILAIFVFSHILFLHVSGSGNPLGINSKGRSLMFTPYFLINDLLGFVVCFIVFLYLVVYVPFFQFDSSNFDEANFLSTPLHIKPEWYFLAAYAVLRCVPNKLGGVYFLFMFLFIYFLIPFLVSSVYIVPLDFFGSVFYFMFICNFVLLTLLGGLPVESPFVSVSVFSSFLYFIYFVLLLDISWLDYVSMIRL 364          
BLAST of cytochrome b vs. TrEMBL
Match: A0A0B4VJ68 (Cytochrome b (Fragment) OS=Schmidtea mediterranea OX=79327 GN=CYTB PE=3 SV=1)

HSP 1 Score: 493.812 bits (1270), Expect = 2.109e-172
Identity = 348/364 (95.60%), Postives = 356/364 (97.80%), Query Frame = 1
Query:    7 LLSNVYLPTPKKIDYFWCXXXXXXXXXXXXXXXXXXXXXXYLPSVYFSFDSVDCFYREVLFGSFFRRVHSIGASXXXXXXXXXXXRGLYFSSYSYVSTWLVGCLILVLSMAIAFLGYVLP*GNMSLWGATVITSLLSAIPYFGSSLVEWL*GGFSISSPTLSRFFVLHFFLPFILAIFVFSHILFLHVSGSGNPLGINSKGRSLMFTPYFLINDLLGXXXXXXXXXXXXXXXXXXXXDSSNFDEANFLSTPLHIKPEWYFLAAYAVLRCVPNKLGGVXXXXXXXXXXXXXXXXVSSVYIVPLDFFGSVFYFMFICNFVLLTLLGGLPXXXXXXXXXXXXXXXXXIYFVLLLDISWLDYVSMIRL 1098
            LLSNVYLPTPK IDYFWCFGSLLLV FVVQFVSGFFLSSFYLPSVYFSFDSVDCFYREVLFGSFFR VHSIGASFFFLFIYFHLFRGLYFSSYSYVSTWLVGCLILVLSMA+AFLGYVLP GNMSLWGATVITSLLSAIPYFGSSLVEWL GGFSISSPTLSRFFVLHFFLPFILAIFVFSHILFLHVSGSGNPLGINSKGRSLMFTPYFLINDLLGFV+CFI+FLYLV+YVPFFQFDSSNFDEANFLSTPLHIKPEWYFLAAYAVLRCVPNKLGGVYFLFMF FIYFLIPFLVSSVYIVPLDFFGS+FYFMFICNFVLLT+LGGLPVESPFV VS FSSFLYF+YFVLLLDISWLDY+SMIRL
Sbjct:    1 LLSNVYLPTPKNIDYFWCFGSLLLVWFVVQFVSGFFLSSFYLPSVYFSFDSVDCFYREVLFGSFFRSVHSIGASFFFLFIYFHLFRGLYFSSYSYVSTWLVGCLILVLSMAVAFLGYVLPWGNMSLWGATVITSLLSAIPYFGSSLVEWLWGGFSISSPTLSRFFVLHFFLPFILAIFVFSHILFLHVSGSGNPLGINSKGRSLMFTPYFLINDLLGFVICFIIFLYLVIYVPFFQFDSSNFDEANFLSTPLHIKPEWYFLAAYAVLRCVPNKLGGVYFLFMFFFIYFLIPFLVSSVYIVPLDFFGSIFYFMFICNFVLLTVLGGLPVESPFVSVSAFSSFLYFVYFVLLLDISWLDYISMIRL 364          
BLAST of cytochrome b vs. TrEMBL
Match: G8EFV3 (Cytochrome b (Fragment) OS=Schmidtea mediterranea OX=79327 GN=CYB PE=4 SV=1)

HSP 1 Score: 337.806 bits (865), Expect = 5.043e-113
Identity = 212/216 (98.15%), Postives = 213/216 (98.61%), Query Frame = 1
Query:  127 YLPSVYFSFDSVDCFYREVLFGSFFRRVHSIGASXXXXXXXXXXXRGLYFSSYSYVSTWLVGCLILVLSMAIAFLGYVLP*GNMSLWGATVITSLLSAIPYFGSSLVEWL*GGFSISSPTLSRFFVLHFFLPFILAIFVFSHILFLHVSGSGNPLGINSKGRSLMFTPYFLINDLLGXXXXXXXXXXXXXXXXXXXXDSSNFDEANFLSTPLHIKP 774
            YLPSVYFSFDSVDCFYREVLFGSFFR VHSIGASFFFLFIYFHLFRGLYFSSYSYVSTWLVGCLILVLSMAIAFLGYVLP GNMSLWGATVITSLLSAIPYFGSSLVEWL GGFSISSPTLSRFFVLHFFLPFILAIFVFSHILFLHVSGSGNPLGINSKGRSLMFTPYFLINDLLGFVVCFI+FLYLVVYVPFFQFDSSNFDEANFLSTPLHIKP
Sbjct:   11 YLPSVYFSFDSVDCFYREVLFGSFFRSVHSIGASFFFLFIYFHLFRGLYFSSYSYVSTWLVGCLILVLSMAIAFLGYVLPWGNMSLWGATVITSLLSAIPYFGSSLVEWLWGGFSISSPTLSRFFVLHFFLPFILAIFVFSHILFLHVSGSGNPLGINSKGRSLMFTPYFLINDLLGFVVCFIIFLYLVVYVPFFQFDSSNFDEANFLSTPLHIKP 226          
BLAST of cytochrome b vs. TrEMBL
Match: G8EFU6 (Cytochrome b (Fragment) OS=Schmidtea mediterranea OX=79327 GN=CYB PE=4 SV=1)

HSP 1 Score: 337.806 bits (865), Expect = 5.099e-113
Identity = 211/216 (97.69%), Postives = 213/216 (98.61%), Query Frame = 1
Query:  127 YLPSVYFSFDSVDCFYREVLFGSFFRRVHSIGASXXXXXXXXXXXRGLYFSSYSYVSTWLVGCLILVLSMAIAFLGYVLP*GNMSLWGATVITSLLSAIPYFGSSLVEWL*GGFSISSPTLSRFFVLHFFLPFILAIFVFSHILFLHVSGSGNPLGINSKGRSLMFTPYFLINDLLGXXXXXXXXXXXXXXXXXXXXDSSNFDEANFLSTPLHIKP 774
            YLPSVYFSFDSVDCFYREVLFGSFFR VHSIGASFFFLFIYFHLFRGLYFSSYSYVSTWLVGCLILVLSMAIAFLGYVLP GNMSLWGATVITSLLSAIPYFGSSLVEWL GGFSISSPTLSRFFVLHFFLPFILAIFVFSHILFLHVSGSGNPLGINSKGRSLMFTPYFLINDLLGFV+CFI+FLYLVVYVPFFQFDSSNFDEANFLSTPLHIKP
Sbjct:   11 YLPSVYFSFDSVDCFYREVLFGSFFRSVHSIGASFFFLFIYFHLFRGLYFSSYSYVSTWLVGCLILVLSMAIAFLGYVLPWGNMSLWGATVITSLLSAIPYFGSSLVEWLWGGFSISSPTLSRFFVLHFFLPFILAIFVFSHILFLHVSGSGNPLGINSKGRSLMFTPYFLINDLLGFVICFIIFLYLVVYVPFFQFDSSNFDEANFLSTPLHIKP 226          
BLAST of cytochrome b vs. TrEMBL
Match: G8EFU9 (Cytochrome b (Fragment) OS=Schmidtea mediterranea OX=79327 GN=CYB PE=4 SV=1)

HSP 1 Score: 337.421 bits (864), Expect = 8.996e-113
Identity = 209/216 (96.76%), Postives = 213/216 (98.61%), Query Frame = 1
Query:  127 YLPSVYFSFDSVDCFYREVLFGSFFRRVHSIGASXXXXXXXXXXXRGLYFSSYSYVSTWLVGCLILVLSMAIAFLGYVLP*GNMSLWGATVITSLLSAIPYFGSSLVEWL*GGFSISSPTLSRFFVLHFFLPFILAIFVFSHILFLHVSGSGNPLGINSKGRSLMFTPYFLINDLLGXXXXXXXXXXXXXXXXXXXXDSSNFDEANFLSTPLHIKP 774
            YLPSVYFSFDSVDCFYREVLFGSFFR VHSIGASFFFLFIYFHLFRGLYFSSYSYVSTWLVGCLILVLSMA+AFLGYVLP GNMSLWGATVITSLLSAIPYFGSSLVEWL GGFSISSPTLSRFFVLHFFLPFILAIFVFSHILFLHVSGSGNPLGINSKGRSLMFTPYFLINDLLGFV+CFI+FLYLV+YVPFFQFDSSNFDEANFLSTPLHIKP
Sbjct:   11 YLPSVYFSFDSVDCFYREVLFGSFFRSVHSIGASFFFLFIYFHLFRGLYFSSYSYVSTWLVGCLILVLSMAVAFLGYVLPWGNMSLWGATVITSLLSAIPYFGSSLVEWLWGGFSISSPTLSRFFVLHFFLPFILAIFVFSHILFLHVSGSGNPLGINSKGRSLMFTPYFLINDLLGFVICFIIFLYLVIYVPFFQFDSSNFDEANFLSTPLHIKP 226          
BLAST of cytochrome b vs. Ensembl Sea Lamprey
Match: CYTB (cytochrome b [Source:NCBI gene;Acc:807810])

HSP 1 Score: 229.565 bits (584), Expect = 2.201e-72
Identity = 152/324 (46.91%), Postives = 200/324 (61.73%), Query Frame = 1
Query:   19 VYLPTPKKIDYFWCXXXXXXXXXXXXXXXXXXXXXXYLPSVYFSFDSVDCFYREVLFGSFFRRVHSIGASXXXXXXXXXXXRGLYFSSYSYVSTWLVGCLILVLSMAIAFLGYVLP*GNMSLWGATVITSLLSAIPYFGSSLVEWL*GGFSISSPTLSRFFVLHFFLPFILAIFVFSHILFLHVSGSGNPLGINSKGRSLMFTPYFLINDLLGXXXXXXXXXXXXXXXXXXXXDSSNFDEANFLSTPLHIKPEWYFLAAYAVLRCVPNKLGGVXXXXXXXXXXXXX-XXXVSSVYIVPLDFFGSVFYFMFICNFVLLTLLGGLP 987
            V LP+P  I  +W FGSLL +  ++Q ++G  L+  Y  +   +F SV    R+V  G   R +H+ GAS FF+ IY H+ RG+Y+ SY Y  TW VG ++  L+ A AF+GYVLP G MS WGATVIT+L+SA+PY G+ +V WL GGFS+S+ TL+RFF  HF LPFILA     HI+FLH +GS NP+GINS    + F PYF   D+LGFV+   +   + +  P    +  NF  AN LSTP HIKPEWYFL AYA+LR VPNKLGGV  L   + I  +I     S    +       + +++ I +  LLT LGG P
Sbjct:   23 VDLPSPANISAWWNFGSLLSLCLILQIITGLILAMHYTANTELAFSSVMHICRDVNNGWLMRNLHANGASMFFICIYAHIGRGIYYGSYLYKETWNVGVILFALTAATAFVGYVLPWGQMSFWGATVITNLISAMPYVGNDIVVWLWGGFSVSNATLTRFFTFHFILPFILAAMTMIHIMFLHQTGSSNPMGINSNLDKIQFHPYFSFKDILGFVILLGILFMISLLAPNALGEPDNFIYANPLSTPPHIKPEWYFLFAYAILRSVPNKLGGVVALAAAIMILLIIPFTHTSKQRGMQFRPLAQITFWILIADLALLTWLGGEP 346          
BLAST of cytochrome b vs. Ensembl Yeast
Match: COB (Cytochrome b; mitochondrially encoded subunit of the ubiquinol-cytochrome c reductase complex which includes Cobp, Rip1p, Cyt1p, Cor1p, Qcr2p, Qcr6p, Qcr7p, Qcr8p, Qcr9p, and Qcr10p [Source:SGD;Acc:S000007270])

HSP 1 Score: 184.882 bits (468), Expect = 1.461e-55
Identity = 125/287 (43.55%), Postives = 173/287 (60.28%), Query Frame = 1
Query:   13 SNVYL----------PTPKKIDYFWCXXXXXXXXXXXXXXXXXXXXXXYLPSVYFSFDSVDCFYREVLFGSFFRRVHSIGASXXXXXXXXXXXRGLYFSSYS--YVSTWLVGCLILVLSMAIAFLGYVLP*GNMSLWGATVITSLLSAIPYFGSSLVEWL*GGFSISSPTLSRFFVLHFFLPFILAIFVFSHILFLHVSGSGNPLGINSKGRSLMFTPYFLINDLLGXXXXXXXXXXXXXXXXXXXXDSSNFDEANFLSTPLHIKPEWYFLAAYAVLRCVPNKLGGV 837
            SNVYL          P P  I+Y+W  GSLL +  V+Q V+G F++  Y  ++  +F SV+   R+V  G   R +H+ GASFFF+ ++ H+ +GLY+ SY    V+ W VG +I +L++A AFLGY    G MS WGATVIT+L SAIP+ G+ +V WL GGFS+S+PT+ RFF LH+ +PFI+A  V  H++ LH+ GS NPLGI      +    YF+  DL+   +  ++    V Y P       N+   N L TP  I PEWY L  YA+LR +P+KL GV
Sbjct:    6 SNVYLSLVNSYIIDSPQPSSINYWWNMGSLLGLCLVIQIVTGIFMAMHYSSNIELAFSSVEHIMRDVHNGYILRYLHANGASFFFMVMFMHMAKGLYYGSYRSPRVTLWNVGVIIFILTIATAFLGYCCVYGQMSHWGATVITNLFSAIPFVGNDIVSWLWGGFSVSNPTIQRFFALHYLVPFIIAAMVIMHLMALHIHGSSNPLGITGNLDRIPMHSYFIFKDLVTVFLFMLILALFVFYSPNTLGHPDNYIPGNPLVTPASIVPEWYLLPFYAILRSIPDKLLGV 292          
BLAST of cytochrome b vs. Ensembl Yeast
Match: BI4 (Mitochondrial mRNA maturase; forms a complex with Nam2p to mediate splicing of the bI4 intron of the COB gene; encoded by both exon and intron sequences of partially processed COB mRNA [Source:SGD;Acc:S000007273])

HSP 1 Score: 157.532 bits (397), Expect = 2.186e-43
Identity = 102/226 (45.13%), Postives = 143/226 (63.27%), Query Frame = 1
Query:   13 SNVYL----------PTPKKIDYFWCXXXXXXXXXXXXXXXXXXXXXXYLPSVYFSFDSVDCFYREVLFGSFFRRVHSIGASXXXXXXXXXXXRGLYFSSYS--YVSTWLVGCLILVLSMAIAFLGYVLP*GNMSLWGATVITSLLSAIPYFGSSLVEWL*GGFSISSPTLSRFFVLHFFLPFILAIFVFSHILFLHVSGSGNPLGINSKGRSLMFTPYFLINDLL 654
            SNVYL          P P  I+Y+W  GSLL +  V+Q V+G F++  Y  ++  +F SV+   R+V  G   R +H+ GASFFF+ ++ H+ +GLY+ SY    V+ W VG +I +L++A AFLGY    G MS WGATVIT+L SAIP+ G+ +V WL GGFS+S+PT+ RFF LH+ +PFI+A  V  H++ LH+ GS NPLGI      +    YF+  DL+
Sbjct:    6 SNVYLSLVNSYIIDSPQPSSINYWWNMGSLLGLCLVIQIVTGIFMAMHYSSNIELAFSSVEHIMRDVHNGYILRYLHANGASFFFMVMFMHMAKGLYYGSYRSPRVTLWNVGVIIFILTIATAFLGYCCVYGQMSHWGATVITNLFSAIPFVGNDIVSWLWGGFSVSNPTIQRFFALHYLVPFIIAAMVIMHLMALHIHGSSNPLGITGNLDRIPMHSYFIFKDLV 231          
BLAST of cytochrome b vs. Ensembl Yeast
Match: BI3 (Mitochondrial mRNA maturase; forms a complex with Mrs1p to mediate splicing of the bI3 intron of the COB gene; encoded by both exon and intron sequences of partially processed COB mRNA [Source:SGD;Acc:S000007272])

HSP 1 Score: 102.449 bits (254), Expect = 2.253e-24
Identity = 76/176 (43.18%), Postives = 108/176 (61.36%), Query Frame = 1
Query:   13 SNVYL----------PTPKKIDYFWCXXXXXXXXXXXXXXXXXXXXXXYLPSVYFSFDSVDCFYREVLFGSFFRRVHSIGASXXXXXXXXXXXRGLYFSSYS--YVSTWLVGCLILVLSMAIAFLGYVLP*GNMSLWGATVITSLLSAIPYFGSSLVEWL*GGFSISSPTLSRFFV 504
            SNVYL          P P  I+Y+W  GSLL +  V+Q V+G F++  Y  ++  +F SV+   R+V  G   R +H+ GASFFF+ ++ H+ +GLY+ SY    V+ W VG +I +L++A AFLGY    G MS WGATVIT+L SAIP+ G+ +V WL GGF++  P  S   +
Sbjct:    6 SNVYLSLVNSYIIDSPQPSSINYWWNMGSLLGLCLVIQIVTGIFMAMHYSSNIELAFSSVEHIMRDVHNGYILRYLHANGASFFFMVMFMHMAKGLYYGSYRSPRVTLWNVGVIIFILTIATAFLGYCCVYGQMSHWGATVITNLFSAIPFVGNDIVSWLWGGFNMEDPYYSNMML 181          
BLAST of cytochrome b vs. Ensembl Yeast
Match: BI2 (Mitochondrial mRNA maturase with a role in splicing; encoded by both exon and intron sequences of partially processed COB mRNA [Source:SGD;Acc:S000007271])

HSP 1 Score: 65.855 bits (159), Expect = 2.184e-12
Identity = 59/144 (40.97%), Postives = 84/144 (58.33%), Query Frame = 1
Query:   13 SNVYL----------PTPKKIDYFWCXXXXXXXXXXXXXXXXXXXXXXYLPSVYFSFDSVDCFYREVLFGSFFRRVHSIGASXXXXXXXXXXXRGLYFSSYS--YVSTWLVGCLILVLSMAIAFLGYVLP*GNMSLWGATVITS 408
            SNVYL          P P  I+Y+W  GSLL +  V+Q V+G F++  Y  ++  +F SV+   R+V  G   R +H+ GASFFF+ ++ H+ +GLY+ SY    V+ W VG +I +L++A AFLGY    G MS WG   I S
Sbjct:    6 SNVYLSLVNSYIIDSPQPSSINYWWNMGSLLGLCLVIQIVTGIFMAMHYSSNIELAFSSVEHIMRDVHNGYILRYLHANGASFFFMVMFMHMAKGLYYGSYRSPRVTLWNVGVIIFILTIATAFLGYCCVYGQMSHWGNMNIAS 149          
BLAST of cytochrome b vs. Ensembl Nematostella
Match: EDO45879 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRT2])

HSP 1 Score: 44.2838 bits (103), Expect = 6.787e-6
Identity = 41/93 (44.09%), Postives = 54/93 (58.06%), Query Frame = 1
Query:   25 LPTPKKIDYFWCXXXXXXXXXXXXXXXXXXXXXXYLPSVYFSFDSVDCFYREVLFGSFFRRVHSIGASXXXXXXXXXXXRGLYFSSYSYVSTW 303
            LP P  + Y W FGSLL +  V+Q  +G FL+  Y   V  +F SVD   R+V +G   R  H+  AS FF+ +Y H+ RGLY+ SYS V  W
Sbjct:   23 LPAPANLSYMWNFGSLLGLCLVIQIATGIFLAMHYCADVSLAFASVDHIMRDVNYGFLLRYAHANXASMFFICLYAHIGRGLYYGSYSKVEVW 115          
BLAST of cytochrome b vs. Ensembl Medaka
Match: CYTB (cytochrome b [Source:NCBI gene;Acc:805442])

HSP 1 Score: 228.794 bits (582), Expect = 1.598e-71
Identity = 150/324 (46.30%), Postives = 203/324 (62.65%), Query Frame = 1
Query:   19 VYLPTPKKIDYFWCXXXXXXXXXXXXXXXXXXXXXXYLPSVYFSFDSVDCFYREVLFGSFFRRVHSIGASXXXXXXXXXXXRGLYFSSYSYVSTWLVGCLILVLSMAIAFLGYVLP*GNMSLWGATVITSLLSAIPYFGSSLVEWL*GGFSISSPTLSRFFVLHFFLPFILAIFVFSHILFLHVSGSGNPLGINSKGRSLMFTPYFLINDLLGXXXXXXXXXXXXXXXXXXXXDSSNFDEANFLSTPLHIKPEWYFLAAYAVLRCVPNKLGGVXXXXXXXXXXXXX-XXXVSSVYIVPLDFFGSVFYFMFICNFVLLTLLGGLP 987
            V LP P  I  +W FGSLL +    Q ++G FL+  Y   +  +F SV    R+V +G   R +H+ GASFFF+ IY H+ RGLY+ SY Y  TW VG ++L+L M  AF+GYVLP G MS WGATVIT+LLSA+PY G++LV+W+ GGFS+ + TL+RFF  HF LPF++A     H++FLH +GS NP G+NS    + F PYF   DLLGF    +  + L ++ P    D  NF  AN L TP HIKPEWYFL AYA+LR +PNKLGGV  L   + + FL+     S    +    F    +++ + + ++LT +GG+P
Sbjct:   19 VDLPAPSNISVWWNFGSLLGLCLAAQIITGLFLAMHYTSDIATAFSSVAHICRDVNYGWLIRNMHANGASFFFICIYLHIGRGLYYGSYLYKETWNVGVILLLLVMMTAFVGYVLPWGQMSFWGATVITNLLSAVPYVGNALVQWIWGGFSVDNATLTRFFAFHFLLPFVIAAATVVHLIFLHETGSNNPTGLNSDSDKVSFHPYFSYKDLLGFAALLVALISLALFSPNLLGDPDNFTPANPLVTPPHIKPEWYFLFAYAILRSIPNKLGGVLALLASILVLFLVPILHTSKQRSLTFRPFTQFLFWLLVADVMVLTWIGGMP 342          
The following BLAST results are available for this feature:
BLAST of cytochrome b vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
MT-CYB6.126e-7145.57mitochondrially encoded cytochrome b [Source:HGNC ... [more]
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BLAST of cytochrome b vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ctb-18.253e-4543.88Cytochrome b [Source:UniProtKB/Swiss-Prot;Acc:P24... [more]
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BLAST of cytochrome b vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mt:Cyt-b1.103e-6951.28gene:FBgn0013678 transcript:FBtr0433502[more]
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BLAST of cytochrome b vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mt-cyb8.955e-7052.01cytochrome b [Source:NCBI gene;Acc:140512][more]
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BLAST of cytochrome b vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
trim338.802e-6952.03tripartite motif containing 33 [Source:Xenbase;Acc... [more]
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BLAST of cytochrome b vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mt-Cytb2.655e-7346.77mitochondrially encoded cytochrome b [Source:MGI S... [more]
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BLAST of cytochrome b vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9BA02|CYB_SIGGR9.307e-7546.91Cytochrome b OS=Sigmops gracilis OX=48457 GN=mt-cy... [more]
sp|Q7YC74|CYB_RHYIS5.006e-7446.18Cytochrome b OS=Rhynchomys isarogensis OX=238000 G... [more]
sp|Q8LWN0|CYB_TAMTE5.556e-7446.18Cytochrome b OS=Tamandua tetradactyla OX=48850 GN=... [more]
sp|Q8SJK5|CYB_GLYMU1.094e-7346.91Cytochrome b OS=Glyptemys muhlenbergii OX=335393 G... [more]
sp|Q7YC63|CYB_APOMU1.600e-7346.46Cytochrome b OS=Apomys musculus OX=238007 GN=MT-CY... [more]
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BLAST of cytochrome b vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
T1PTK75.166e-17498.35Cytochrome b (Fragment) OS=Schmidtea mediterranea ... [more]
A0A0B4VJ682.109e-17295.60Cytochrome b (Fragment) OS=Schmidtea mediterranea ... [more]
G8EFV35.043e-11398.15Cytochrome b (Fragment) OS=Schmidtea mediterranea ... [more]
G8EFU65.099e-11397.69Cytochrome b (Fragment) OS=Schmidtea mediterranea ... [more]
G8EFU98.996e-11396.76Cytochrome b (Fragment) OS=Schmidtea mediterranea ... [more]
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BLAST of cytochrome b vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of cytochrome b vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
CYTB2.201e-7246.91cytochrome b [Source:NCBI gene;Acc:807810][more]
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BLAST of cytochrome b vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 4
Match NameE-valueIdentityDescription
COB1.461e-5543.55Cytochrome b; mitochondrially encoded subunit of t... [more]
BI42.186e-4345.13Mitochondrial mRNA maturase; forms a complex with ... [more]
BI32.253e-2443.18Mitochondrial mRNA maturase; forms a complex with ... [more]
BI22.184e-1240.97Mitochondrial mRNA maturase with a role in splicin... [more]
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BLAST of cytochrome b vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO458796.787e-644.09Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of cytochrome b vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
CYTB1.598e-7146.30cytochrome b [Source:NCBI gene;Acc:805442][more]
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BLAST of cytochrome b vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 0
Match NameE-valueIdentityDescription
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Cross References
External references for this transcript
DatabaseAccession
SmedGD GBrowseSMED30000702
GenBankYP_008592437.2
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30000702 ID=SMED30000702|Name=cytochrome b|organism=Schmidtea mediterranea sexual|type=transcript|length=1101bp
tattgtttgttatctaatgtctatttacctactcctaagaaaatagatta
tttttggtgttttggttctttattgttagtttgatttgtagttcagtttg
tttctggcttctttttatcttctttctatttgccttctgtttatttttct
tttgattcggttgattgtttttatcgtgaagttttatttggttctttctt
tcgtagggttcatagtattggggcttctttcttttttttatttatttatt
ttcatttgtttcgtggtttgtatttttctagttatagttatgtttctact
tggttggttggttgtttgattcttgttttgagtatggcaatagctttttt
aggttatgttttgccttgaggtaatatgtctctttggggagctactgtta
ttactagtttattgtcagctattccttattttggatcttctttagttgag
tggttgtgaggtggtttttctatttcttctcctacgttgtctcgtttctt
tgttttgcattttttcttgccttttattttggctatatttgtcttctctc
atattttgtttttacatgtgagtggtagtggtaatcctcttggtattaat
tcaaagggtcgttctcttatgtttactccttattttttaattaatgattt
attaggttttgttgtgtgttttattgtttttttgtatttggttgtttatg
ttcctttctttcaatttgatagttctaattttgatgaagctaattttctt
tcaactcctttacatattaagcctgagtggtattttttagctgcttatgc
agttttacgttgtgttcctaataagttgggaggagtttatttcttattta
tgtttttgtttatttattttttgattccttttttagtttcttctgtttat
attgttcctttggatttctttggatcggttttttattttatgtttatttg
taattttgttttattgactttgttgggtggtttgcctgttgaatctccat
ttgttagggttagtgtatttagttctttcttatattttatttattttgtt
ctgttattagatatttcatggttagattatgtttcaatgattcgtttgta
g
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000039gamma neoblast
PLANA:0000101muscle cell
PLANA:0000429neoblast
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR027387Cytb/b6-like
IPR005797Cyt_b/b6_N
IPR016174Di-haem_cyt_TM
IPR005798Cyt_b/b6_C
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0005739mitochondrion
GO:0005743mitochondrial inner membrane
GO:0016021integral component of membrane
GO:0070469respiratory chain
Vocabulary: molecular function
TermDefinition
GO:0016491oxidoreductase activity
GO:0009055electron carrier activity
GO:0046872metal ion binding
Vocabulary: biological process
TermDefinition
GO:0022904respiratory electron transport chain
GO:0055114oxidation-reduction process