SMED30000648

Overview
NameSMED30000648
Smed IDSMED30000648
Length (bp)2137
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30000648 (SMED30000648) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30000648 (SMED30000648) in cells (dots) of each of the 12 neoblast clusters.

 

back to top


 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30000648 (SMED30000648) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30000648 (SMED30000648) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top


 

Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020




SMED30000648

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
headSMED30000648SMESG000018727.1 SmedASXL_004922SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
headSMED30000648SMESG000018727.1 SmedASXL_019700SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
headSMED30000648SMESG000018727.1 dd_Smed_v6_25826_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of SMED30000648 vs. TrEMBL
Match: A0A158QE86 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=HDID_LOCUS7029 PE=4 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 2.754e-30
Identity = 77/228 (33.77%), Postives = 116/228 (50.88%), Query Frame = 1
Query:   52 DEFRVVYDGNCINQGGNRFCRNKVLNSFCDNLENICLCLPGYVAIQESMGKLCQPVLGNSRCQIDSHCSNLTNKSTCHPGVGLCICPADTMFVLQTNACEKVIKMADNSTFCSRCQGEGGECYIANYEISFKQNNTQ----CACPYRTATSA----------TIDNWKGSVICSVLLANIGEYCNQKRVICGSSKAECSYSEAFEAEVCQCPPKHSIVKKNFGDFYQC 693
            DE+ VV  G C+++G + +C  +V NSFC   +N C C P YVAIQE  G  C+P+L +  C +D  C ++ N S CHPG G C CP  T++V Q +AC  + +   N++FC +C    G C+ A+        N      C+CPY+TA             ++  W     C  +L ++GE CN   +IC    AECS S   +   C+C   +  V +    +++C
Sbjct:   30 DEYHVVASGPCVDEG-DAYCIKRVPNSFCSVTKNECFCQPAYVAIQEEYGITCKPLLTSLSCHVDKDCIHVKN-SICHPGAGFCACPGGTVYVAQEHACRNLAEKQTNNSFCEKCTQINGICFYADTPDKGDSENKTPKFGCSCPYKTAMPMPKQVYASNDQSLSPW---AFCRAILVDVGELCNDADLICRPRNAECS-SRNGDPRTCRCKSGYFPVYQQSLHYHEC 251          
BLAST of SMED30000648 vs. TrEMBL
Match: A0A5K4FDJ1 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 3.923e-27
Identity = 86/259 (33.20%), Postives = 123/259 (47.49%), Query Frame = 1
Query:   97 GNRFCRNKVLNSFCDNLENICLCLPGYVAIQESMGKLCQPVLGNSRCQIDSHCSNLTNKSTCHPGVGLCICPADTMFVLQTNACEKVIKMADNSTFCSRCQGEGGECY--------IANYEISFKQNNT----QCACPYRTATSATIDNWKGSVI--------------------------CSVLLANIGEYCNQKRVICGSSKAECS----YSEAFEAEVCQCPPKHSIVKKNFGDFYQCQPNIHVQTACNNCQMLTQ 747
            G+ FC   V NSFC   +N C C  G+ +IQE  G +C+ +L N +CQ+DS C  + N S CHPG G CICP+ T+++ +  AC   IK   N  +C +CQ   G CY        I N+ +++ Q N     +C CPY T +  T   W  S I                          C  LL +IG +CNQ  ++C SS A CS     +  + +  C C   +  V +++ D+Y+C P      ACN C  + Q
Sbjct:   36 GDSFCTELVPNSFCSIEKNECFCKYGHYSIQEDDGIICKTLLTNDKCQLDSDCVYVKN-SICHPGAGACICPSGTIYIPEEQACRFQIKTFTND-YCDKCQRFHGLCYRNEEDQGIIRNFSLAYSQFNERIPYRCKCPYNTVSINTNLLWNKSYINQSKNLISVDDIQILDYMNKKENIGFCRGLLVDIGMFCNQIDMLCRSSNAFCSNGKMINNVYLSPQCVCQDGYLPVYQDYLDYYECIP------ACNRCYSIEQ 286          
BLAST of SMED30000648 vs. TrEMBL
Match: A0A0R3U7J3 (Uncharacterized protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS2797 PE=4 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 6.141e-27
Identity = 72/210 (34.29%), Postives = 106/210 (50.48%), Query Frame = 1
Query:   25 CQISSNSLT--DEFRVVYDGNCINQGGNRFCRNKVLNSFCDNLENICLCLPGYVAIQESMGKLCQPVLGNSRCQIDSHCSNLTNKSTCHPGVGLCICPADTMFVLQTNACEKVIKMADNSTFCSRCQGEGGECYIANYEISFKQNNTQ-----CACPYRTATSA---------TIDNWKGSVICSVLLANIGEYCNQKRVICGSSKAECS 606
            C ++  +LT  DE+ VV  G C+ +G + +C  +V NS C   +N C C P +VAIQE  G  C+P+L +  C +D  C ++ N S CHPG G C CP  T++V Q +AC  +    +NSTFC  C+   G CY           N+      C CP+ TA  +          + +W     C  +L +IG  CNQ  ++C    +EC+
Sbjct:   11 CLVAWPALTHADEYHVVAAGPCVAEG-DAYCIRRVTNSVCSATKNECFCQPSFVAIQEEYGITCKPLLTSLSCHVDRDCVHVRN-SICHPGAGFCACPGGTIYVPQAHACRNLADK-ENSTFCETCRQVNGVCYYEEQPGGSNNRNSSSPGLGCTCPFDTAMPSPSPDAASKEPVPHW---AFCKAILVDIGGLCNQIDLLCRPRNSECT 214          
BLAST of SMED30000648 vs. TrEMBL
Match: A0A0R3T769 (Uncharacterized protein OS=Rodentolepis nana OX=102285 GN=HNAJ_LOCUS2906 PE=4 SV=1)

HSP 1 Score: 120.939 bits (302), Expect = 1.257e-26
Identity = 76/228 (33.33%), Postives = 117/228 (51.32%), Query Frame = 1
Query:   46 LTDEFRVVYDGNCINQGGNRFCRNKVLNSFCDNLENICLCLPGYVAIQESMGKLCQPVLGNSRCQIDSHCSNLTNKSTCHPGVGLCICPADTMFVLQTNACEKVIKMAD-NSTFCSRCQGEGGECYIANY-EISFKQNNTQ-----CACPYRTATSATIDNWKGSVICSVLLANIGEYCNQKRVICGSSKAECSYSE-----AFEAEVCQCPPKHSIVKKNFGDFYQC 693
            L  +  +V+ G C N+    FC   V NS C+  +N C C  G+VA++E     C+ +L + +C++D+ C ++ N+S+CHPG G C CP +T+FV Q NAC    K+ D  S  C+ C  EGG+C+   + +   ++ +TQ     C CP     +   D  +    C   LA+IG+ CN++ + C S  A C   E     A     C CPP +  V +    FY+C
Sbjct:   35 LCSDEHLVFTGAC-NKEPPNFCDRIVKNSVCNRAKNECFCRKGFVAVKEGDSVACKTLLTDLKCRVDADCVHV-NRSSCHPGAGYCTCPGNTIFVPQINACRT--KLFDRKSLICASCLQEGGDCFAFEHGDHIHEEGSTQFERYGCVCPNLRTNTQFRDPSRPQRTCGAQLADIGDLCNEEDLRCRSPIATCRIIEEAQWGAMTYGTCTCPPDYIPVFQKLLRFYEC 258          
BLAST of SMED30000648 vs. TrEMBL
Match: A0A068YE62 (Uncharacterized protein OS=Echinococcus multilocularis OX=6211 GN=EmuJ_000926400 PE=4 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 2.058e-26
Identity = 76/223 (34.08%), Postives = 112/223 (50.22%), Query Frame = 1
Query:   55 EFRVVYDGNCINQGGNRFCRNKVLNSFCDNLENICLCLPGYVAIQESMGKLCQPVLGNSRCQIDSHCSNLTNKSTCHPGVGLCICPADTMFVLQTNACEKVIKMADNSTFCSRCQGEGGECYIANYEISFKQNNTQCACPYRTAT----------SATIDNWKGSVICSVLLANIGEYCNQKRVICGSSKAECSYSEAFEAEVCQCPPKHSIVKKNFGDFYQC 693
              R +  G C+ +G + +C  +V NSFC   +N C C P +VAIQE  G  C+P+L +  C +D  C ++ N S CHPG G C CP  T +V Q +AC  V + ++NS FC  C+   G CY A    + K+    CACP+ TA              I  W     C  +L ++G  CNQ+ ++C    +ECS S     + C+C P    V +    +++C
Sbjct:   27 RLRSLDLGPCVEEG-DFYCSRRVPNSFCSVTKNECFCHPAFVAIQEEYGITCKPLLTSLNCHVDKDCVHVKN-SICHPGAGFCACPGGTAYVAQEHACHNVAENSNNS-FCETCKQMNGICYHAE---NSKKQEFGCACPFNTAMPLPQRLHASNGGPIAQW---AFCKAILVDVGHLCNQEDLLCRPRNSECS-SLNGGPKSCRCKPGFFPVYQQSLHYHEC 239          
BLAST of SMED30000648 vs. Planmine SMEST
Match: SMESG000018727.1 (SMESG000018727.1)

HSP 1 Score: 1384.39 bits (3582), Expect = 0.000e+0
Identity = 698/699 (99.86%), Postives = 699/699 (100.00%), Query Frame = 1
Query:    1 GFFILLQICQISSNSLTDEFRVVYDGNCINQGGNRFCRNKVLNSFCDNLENICLCLPGYVAIQESMGKLCQPVLGNSRCQIDSHCSNLTNKSTCHPGVGLCICPADTMFVLQTNACEKVIKMADNSTFCSRCQGEGGECYIANYEISFKQNNTQCACPYRTATSATIDNWKGSVICSVLLANIGEYCNQKRVICGSSKAECSYSEAFEAEVCQCPPKHSIVKKNFGDFYQCQPNIHVQTACNNCQMLTQVSTESPESILFTVSCTSYQIRLSLDVDQKLLLSDESILFVFGTRPGNNYEFLNCKFKKPSSLHNRYYWSIEIDKAAKQMPCGIYRVFYLNGALFTFYVAIWNKQDRVDLIKDHLYELSCRLFEKSMDIESEIISSQILDRSWINYGDQSANFYSLNRKKKGKINFQNSLNLYRNQFQKEESIHIIIRFDEEYMSRRNRKYNYLDVESCSVIDEKYEKNTSPVVMIELFNKDFVTISDKNYQYKRSHLISFNLSNQNSVNRNSIIIKCIVRLCEKELWCSANSNVLLPKPNVEFEVLKKKLIIINTIQSTRVEFVDVHKEQFDSQKCPSFVCLTNVQVXXXXXXXXXXXXXXXXXXXXXXAQIYKTKQSDLSHQISKNPISDYIDLKYLPDSNNLILNDENIHQTPLKDIRYHSEESLDNLHQTNQVNLFNIIAATINPPSTDHRSTEIYV 2097
            GFFILLQICQISSNSLTDEFRVVYDGNCINQGGNRFCRNKVLNSFCDNLENICLCLPGYVAIQESMGKLCQPVLGNSRCQIDSHCSNLTNKSTCHPGVGLCICPADTMFVLQTNACEKVIKMADNSTFCSRCQGEGGECYIANYEISFKQNNTQCACPYRTATSATIDNWKGSVICSVLLANIGEYCNQKRVICGSSKAECSYSEAFEAEVCQCPPKHSIVKKNFGDFYQCQPNIHVQTACNNCQMLTQVSTESPESILFTVSCTSYQIRLSLDVDQKLLLSDESILFVFGTRPGNNYEFLNCKFKKPSSLHNRYYWSIEIDKAAKQMPCGIYRVFYLNGALFTFYVAIWNKQDRVDLIKDHLYELSCRLFEKSMDIESEIISSQILDRSWINYGDQSANFYSLNRKKKGKINFQNSLNLYRNQFQKEESIHIIIRFDEEYM+RRNRKYNYLDVESCSVIDEKYEKNTSPVVMIELFNKDFVTISDKNYQYKRSHLISFNLSNQNSVNRNSIIIKCIVRLCEKELWCSANSNVLLPKPNVEFEVLKKKLIIINTIQSTRVEFVDVHKEQFDSQKCPSFVCLTNVQVLLIIFITSLISIFFIVSLSILLAQIYKTKQSDLSHQISKNPISDYIDLKYLPDSNNLILNDENIHQTPLKDIRYHSEESLDNLHQTNQVNLFNIIAATINPPSTDHRSTEIYV
Sbjct:    3 GFFILLQICQISSNSLTDEFRVVYDGNCINQGGNRFCRNKVLNSFCDNLENICLCLPGYVAIQESMGKLCQPVLGNSRCQIDSHCSNLTNKSTCHPGVGLCICPADTMFVLQTNACEKVIKMADNSTFCSRCQGEGGECYIANYEISFKQNNTQCACPYRTATSATIDNWKGSVICSVLLANIGEYCNQKRVICGSSKAECSYSEAFEAEVCQCPPKHSIVKKNFGDFYQCQPNIHVQTACNNCQMLTQVSTESPESILFTVSCTSYQIRLSLDVDQKLLLSDESILFVFGTRPGNNYEFLNCKFKKPSSLHNRYYWSIEIDKAAKQMPCGIYRVFYLNGALFTFYVAIWNKQDRVDLIKDHLYELSCRLFEKSMDIESEIISSQILDRSWINYGDQSANFYSLNRKKKGKINFQNSLNLYRNQFQKEESIHIIIRFDEEYMARRNRKYNYLDVESCSVIDEKYEKNTSPVVMIELFNKDFVTISDKNYQYKRSHLISFNLSNQNSVNRNSIIIKCIVRLCEKELWCSANSNVLLPKPNVEFEVLKKKLIIINTIQSTRVEFVDVHKEQFDSQKCPSFVCLTNVQVLLIIFITSLISIFFIVSLSILLAQIYKTKQSDLSHQISKNPISDYIDLKYLPDSNNLILNDENIHQTPLKDIRYHSEESLDNLHQTNQVNLFNIIAATINPPSTDHRSTEIYV 701          
BLAST of SMED30000648 vs. Planmine SMEST
Match: SMESG000018727.1 (SMESG000018727.1)

HSP 1 Score: 1379.77 bits (3570), Expect = 0.000e+0
Identity = 698/700 (99.71%), Postives = 699/700 (99.86%), Query Frame = 1
Query:    1 GFFILLQICQISSNSLTDEFRVVYDGNCINQGGNRFCRNKVLNSFCDNLENICLCLPGYVAIQESMGKLCQPVLGNSRCQIDSHCSNLTNKSTCHPGVGLCICPADTMFVLQTNACEKVIKMADNSTFCSRCQGEGGECYIANYEIS-FKQNNTQCACPYRTATSATIDNWKGSVICSVLLANIGEYCNQKRVICGSSKAECSYSEAFEAEVCQCPPKHSIVKKNFGDFYQCQPNIHVQTACNNCQMLTQVSTESPESILFTVSCTSYQIRLSLDVDQKLLLSDESILFVFGTRPGNNYEFLNCKFKKPSSLHNRYYWSIEIDKAAKQMPCGIYRVFYLNGALFTFYVAIWNKQDRVDLIKDHLYELSCRLFEKSMDIESEIISSQILDRSWINYGDQSANFYSLNRKKKGKINFQNSLNLYRNQFQKEESIHIIIRFDEEYMSRRNRKYNYLDVESCSVIDEKYEKNTSPVVMIELFNKDFVTISDKNYQYKRSHLISFNLSNQNSVNRNSIIIKCIVRLCEKELWCSANSNVLLPKPNVEFEVLKKKLIIINTIQSTRVEFVDVHKEQFDSQKCPSFVCLTNVQVXXXXXXXXXXXXXXXXXXXXXXAQIYKTKQSDLSHQISKNPISDYIDLKYLPDSNNLILNDENIHQTPLKDIRYHSEESLDNLHQTNQVNLFNIIAATINPPSTDHRSTEIYV 2097
            GFFILLQICQISSNSLTDEFRVVYDGNCINQGGNRFCRNKVLNSFCDNLENICLCLPGYVAIQESMGKLCQPVLGNSRCQIDSHCSNLTNKSTCHPGVGLCICPADTMFVLQTNACEKVIKMADNSTFCSRCQGEGGECYIANYEIS FKQNNTQCACPYRTATSATIDNWKGSVICSVLLANIGEYCNQKRVICGSSKAECSYSEAFEAEVCQCPPKHSIVKKNFGDFYQCQPNIHVQTACNNCQMLTQVSTESPESILFTVSCTSYQIRLSLDVDQKLLLSDESILFVFGTRPGNNYEFLNCKFKKPSSLHNRYYWSIEIDKAAKQMPCGIYRVFYLNGALFTFYVAIWNKQDRVDLIKDHLYELSCRLFEKSMDIESEIISSQILDRSWINYGDQSANFYSLNRKKKGKINFQNSLNLYRNQFQKEESIHIIIRFDEEYM+RRNRKYNYLDVESCSVIDEKYEKNTSPVVMIELFNKDFVTISDKNYQYKRSHLISFNLSNQNSVNRNSIIIKCIVRLCEKELWCSANSNVLLPKPNVEFEVLKKKLIIINTIQSTRVEFVDVHKEQFDSQKCPSFVCLTNVQVLLIIFITSLISIFFIVSLSILLAQIYKTKQSDLSHQISKNPISDYIDLKYLPDSNNLILNDENIHQTPLKDIRYHSEESLDNLHQTNQVNLFNIIAATINPPSTDHRSTEIYV
Sbjct:    3 GFFILLQICQISSNSLTDEFRVVYDGNCINQGGNRFCRNKVLNSFCDNLENICLCLPGYVAIQESMGKLCQPVLGNSRCQIDSHCSNLTNKSTCHPGVGLCICPADTMFVLQTNACEKVIKMADNSTFCSRCQGEGGECYIANYEISRFKQNNTQCACPYRTATSATIDNWKGSVICSVLLANIGEYCNQKRVICGSSKAECSYSEAFEAEVCQCPPKHSIVKKNFGDFYQCQPNIHVQTACNNCQMLTQVSTESPESILFTVSCTSYQIRLSLDVDQKLLLSDESILFVFGTRPGNNYEFLNCKFKKPSSLHNRYYWSIEIDKAAKQMPCGIYRVFYLNGALFTFYVAIWNKQDRVDLIKDHLYELSCRLFEKSMDIESEIISSQILDRSWINYGDQSANFYSLNRKKKGKINFQNSLNLYRNQFQKEESIHIIIRFDEEYMARRNRKYNYLDVESCSVIDEKYEKNTSPVVMIELFNKDFVTISDKNYQYKRSHLISFNLSNQNSVNRNSIIIKCIVRLCEKELWCSANSNVLLPKPNVEFEVLKKKLIIINTIQSTRVEFVDVHKEQFDSQKCPSFVCLTNVQVLLIIFITSLISIFFIVSLSILLAQIYKTKQSDLSHQISKNPISDYIDLKYLPDSNNLILNDENIHQTPLKDIRYHSEESLDNLHQTNQVNLFNIIAATINPPSTDHRSTEIYV 702          
BLAST of SMED30000648 vs. Planmine SMEST
Match: SMESG000020498.1 (SMESG000020498.1)

HSP 1 Score: 184.882 bits (468), Expect = 8.168e-49
Identity = 154/570 (27.02%), Postives = 249/570 (43.68%), Query Frame = 1
Query:   52 DEFRVVYDGNCINQGGNRFCRNKVLNSFCDNLENICLCLPGYVAIQESMGKLCQPVLGNSRCQIDSHCSNLTNKSTCHPGVGLCICPADTMFVLQTNACEKVIKMADNSTFCSRCQGEGGECYI-ANYEISFKQNN---TQCACPYRTATSATIDNWKGSVICSVLLANIGEYCNQKRVICGSSKAECSYSEAFEAEVCQCPPKHSIVKKNFGDFYQC----------------------------QPNIHVQTACNNCQMLTQVSTESPESILFT--VSCTSYQIRLSLDVDQKLLLSDESILFVFGTRPG----------NNYEFLNCKFKKPSSLHNRYYWSIEIDKAAKQMP---CGIYRVFYLNGALFTFYVAIWNKQDRVDLIKDHLYELSCRLFEKSMDIESEIISSQILDRSWINYGD--QSANFYSLNRKKKGKINFQNSLNLYRNQFQKEESIHIIIRFDEEYMSRRNRKYNYLDVESCSVIDEK-----YEKNTSPVVMIELFNKDF---VTISDKNYQYKRSHLISFNLSNQNSVNRNSIIIKCIVRLCEKELWCSAN 1590
            D+++VVY G C+++    +C NKV NS+C    N C C  GYV ++E  G +C+P+L   +C+ D+ CS +TN S CHPG G C CP+  +FV   N C   ++ + N   C  C   GG C+   N  +  K +N   +QC+CP+ T T     + K   IC  +  N+GEYCN   +IC S  ++C YS  + A +CQC   +  V +     + C                              N+ ++  C+  Q    V+  S + +  T  + C S +I + L++        + + ++  T              N     CK  +    + +   S+  D      P   CGI    YLN      Y+ +WN+   +D+  D L++L+C+    S+D E+ +++ QILDRSW   G       F SL+      I   N L+    +F   ++I I  +       +R  +  Y  VE CS I        Y++     + I+   + F   +  S  N+  ++   IS      N     +++  CI++LCE   WCS N
Sbjct:   18 DKYQVVYRGPCLSKPS-YYCHNKVKNSYCSKQNNECFCSFGYVTVRERSGVICKPLLSEMKCRFDAECSEITNSSICHPGAGSCTCPSGMIFVSHQNFCRNYLRNSTNE-ICYHCSRLGGICFSEENLNLFPKTSNIIKSQCSCPFNTMTPNAKKSIKKYQICMAIRVNLGEYCNDDDLICISKNSKCIYSHEYLATICQCKTGYLAVYEKKESHFNCYQLVSDIEYPRCSLCYSSGGTCIIQVKSSRNVVLKCLCDKYQYF-DVTGHSIQCMKVTAGIKCQSQKIEICLNIPTNYEYYAQLLDYIRRTPKAISISDAYSHRKNISKKICKMNEYKISYEKTVASLCSDFEINTKPSDHCGISNGNYLNINFVGLYMRVWNQPYIIDIANDFLFDLTCK---TSVDQEN-LVNGQILDRSWTGTGKVILPDVFISLDDTVDMYITNLNGLSY---EFVSLQNIQIHFK-----SFKRKDELPYFLVEKCSEIYSDSQITIYQRLHFSEIKIKHKIRGFTRHLLNSSTNFHDQKILFISSEFQIPNVSAVTNVLYSCIIKLCEDLFWCSQN 572          
BLAST of SMED30000648 vs. Planmine SMEST
Match: SMESG000020498.1 (SMESG000020498.1)

HSP 1 Score: 184.111 bits (466), Expect = 1.283e-48
Identity = 153/562 (27.22%), Postives = 247/562 (43.95%), Query Frame = 1
Query:   52 DEFRVVYDGNCINQGGNRFCRNKVLNSFCDNLENICLCLPGYVAIQESMGKLCQPVLGNSRCQIDSHCSNLTNKSTCHPGVGLCICPADTMFVLQTNACEKVIKMADNSTFCSRCQGEGGECYI-ANYEISFKQNN---TQCACPYRTATSATIDNWKGSVICSVLLANIGEYCNQKRVICGSSKAECSYSEAFEAEVCQCPPKHSIVKKNFGDFYQC----------------------------QPNIHVQTACNNCQMLTQVSTESPESILFT--VSCTSYQIRLSLDVDQKLLLSDESILFVFGTRPG----------NNYEFLNCKFKKPSSLHNRYYWSIEIDKAAKQMP---CGIYRVFYLNGALFTFYVAIWNKQDRVDLIKDHLYELSCRLFEKSMDIESEIISSQILDRSWINYGD--QSANFYSLNRKKKGKINFQNSLNLYRNQFQKEESIHIIIRFDEEYMSRRNRKYNYLDVESCSVIDEKYEKNTSPVVMIELFNKDFVTISDKNYQYKRSHLISFNLSNQNSVNRNSIIIKCIVRLCEKELWCSAN 1590
            D+++VVY G C+++    +C NKV NS+C    N C C  GYV ++E  G +C+P+L   +C+ D+ CS +TN S CHPG G C CP+  +FV   N C   ++ + N   C  C   GG C+   N  +  K +N   +QC+CP+ T T     + K   IC  +  N+GEYCN   +IC S  ++C YS  + A +CQC   +  V +     + C                              N+ ++  C+  Q    V+  S + +  T  + C S +I + L++        + + ++  T              N     CK  +    + +   S+  D      P   CGI    YLN      Y+ +WN+   +D+  D L++L+C+    S+D E+ +++ QILDRSW   G       F SL+      I   N L+    +F   ++I I  +       +R  +  Y  VE CS                E+++   +TI      Y+ S    F + N ++V   +++  CI++LCE   WCS N
Sbjct:   18 DKYQVVYRGPCLSKPS-YYCHNKVKNSYCSKQNNECFCSFGYVTVRERSGVICKPLLSEMKCRFDAECSEITNSSICHPGAGSCTCPSGMIFVSHQNFCRNYLRNSTNE-ICYHCSRLGGICFSEENLNLFPKTSNIIKSQCSCPFNTMTPNAKKSIKKYQICMAIRVNLGEYCNDDDLICISKNSKCIYSHEYLATICQCKTGYLAVYEKKESHFNCYQLVSDIEYPRCSLCYSSGGTCIIQVKSSRNVVLKCLCDKYQYF-DVTGHSIQCMKVTAGIKCQSQKIEICLNIPTNYEYYAQLLDYIRRTPKAISISDAYSHRKNISKKICKMNEYKISYEKTVASLCSDFEINTKPSDHCGISNGNYLNINFVGLYMRVWNQPYIIDIANDFLFDLTCK---TSVDQEN-LVNGQILDRSWTGTGKVILPDVFISLDDTVDMYITNLNGLSY---EFVSLQNIQIHFK-----SFKRKDELPYFLVEKCS----------------EIYSDSQITI------YQSSE---FQIPNVSAV--TNVLYSCIIKLCEDLFWCSQN 537          
BLAST of SMED30000648 vs. Planmine SMEST
Match: SMESG000020498.1 (SMESG000020498.1)

HSP 1 Score: 177.178 bits (448), Expect = 2.352e-46
Identity = 154/564 (27.30%), Postives = 245/564 (43.44%), Query Frame = 1
Query:   52 DEFRVVYDGNCINQGGNRFCRNKVLNSFCDNLENICLCLPGYVAIQESMGKLCQPVLGNSRCQIDSHCSNLTNKSTCHPGVGLCICPADTMFVLQTNACEKVIKMADNST--FCSRCQGEGGECYI-ANYEISFKQNN---TQCACPYRTATSATIDNWKGSVICSVLLANIGEYCNQKRVICGSSKAECSYSEAFEAEVCQCPPKHSIVKKNFGDFYQC----------------------------QPNIHVQTACNNCQMLTQVSTESPESILFT--VSCTSYQIRLSLDVDQKLLLSDESILFVFGTRPG----------NNYEFLNCKFKKPSSLHNRYYWSIEIDKAAKQMP---CGIYRVFYLNGALFTFYVAIWNKQDRVDLIKDHLYELSCRLFEKSMDIESEIISSQILDRSWINYGD--QSANFYSLNRKKKGKINFQNSLNLYRNQFQKEESIHIIIRFDEEYMSRRNRKYNYLDVESCSVIDEKYEKNTSPVVMIELFNKDFVTISDKNYQYKRSHLISFNLSNQNSVNRNSIIIKCIVRLCEKELWCSAN 1590
            D+++VVY G C+++    +C NKV NS+C    N C C  GYV ++E  G +C+P+L   +C+ D+ CS +TN S CHPG G C CP+  +FV   N          NST   C  C   GG C+   N  +  K +N   +QC+CP+ T T     + K   IC  +  N+GEYCN   +IC S  ++C YS  + A +CQC   +  V +     + C                              N+ ++  C+  Q    V+  S + +  T  + C S +I + L++        + + ++  T              N     CK  +    + +   S+  D      P   CGI    YLN      Y+ +WN+   +D+  D L++L+C+    S+D E+ +++ QILDRSW   G       F SL+      I   N L+    +F   ++I I  +       +R  +  Y  VE CS                E+++   +TI      Y+ S    F + N ++V   +++  CI++LCE   WCS N
Sbjct:   18 DKYQVVYRGPCLSKPS-YYCHNKVKNSYCSKQNNECFCSFGYVTVRERSGVICKPLLSEMKCRFDAECSEITNSSICHPGAGSCTCPSGMIFVSHQN-----FNYLRNSTNEICYHCSRLGGICFSEENLNLFPKTSNIIKSQCSCPFNTMTPNAKKSIKKYQICMAIRVNLGEYCNDDDLICISKNSKCIYSHEYLATICQCKTGYLAVYEKKESHFNCYQLVSDIEYPRCSLCYSSGGTCIIQVKSSRNVVLKCLCDKYQYF-DVTGHSIQCMKVTAGIKCQSQKIEICLNIPTNYEYYAQLLDYIRRTPKAISISDAYSHRKNISKKICKMNEYKISYEKTVASLCSDFEINTKPSDHCGISNGNYLNINFVGLYMRVWNQPYIIDIANDFLFDLTCK---TSVDQEN-LVNGQILDRSWTGTGKVILPDVFISLDDTVDMYITNLNGLSY---EFVSLQNIQIHFK-----SFKRKDELPYFLVEKCS----------------EIYSDSQITI------YQSSE---FQIPNVSAV--TNVLYSCIIKLCEDLFWCSQN 535          
The following BLAST results are available for this feature:
BLAST of SMED30000648 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30000648 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30000648 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30000648 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30000648 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30000648 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30000648 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30000648 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A158QE862.754e-3033.77Uncharacterized protein OS=Hymenolepis diminuta OX... [more]
A0A5K4FDJ13.923e-2733.20Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
A0A0R3U7J36.141e-2734.29Uncharacterized protein OS=Mesocestoides corti OX=... [more]
A0A0R3T7691.257e-2633.33Uncharacterized protein OS=Rodentolepis nana OX=10... [more]
A0A068YE622.058e-2634.08Uncharacterized protein OS=Echinococcus multilocul... [more]
back to top
BLAST of SMED30000648 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30000648 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30000648 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30000648 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30000648 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30000648 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000018727.10.000e+099.86SMESG000018727.1[more]
SMESG000018727.10.000e+099.71SMESG000018727.1[more]
SMESG000020498.18.168e-4927.02SMESG000020498.1[more]
SMESG000020498.11.283e-4827.22SMESG000020498.1[more]
SMESG000020498.12.352e-4627.30SMESG000020498.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30000648 ID=SMED30000648|Name=SMED30000648|organism=Schmidtea mediterranea sexual|type=transcript|length=2137bp
GGATTTTTTATACTGTTACAAATTTGTCAAATTTCGAGCAATTCTCTCAC
AGATGAATTTCGTGTTGTCTATGATGGAAATTGCATCAACCAAGGCGGAA
ATCGATTTTGTCGAAACAAAGTTCTGAACAGTTTCTGTGACAATTTGGAA
AATATTTGTCTCTGTCTACCTGGCTATGTTGCCATTCAAGAATCAATGGG
GAAATTATGCCAACCAGTTTTGGGTAATTCTCGCTGTCAAATTGATTCTC
ATTGTTCAAATCTCACAAACAAAAGTACCTGCCATCCTGGAGTAGGTCTT
TGCATTTGTCCAGCGGATACGATGTTTGTTCTACAAACTAACGCATGCGA
GAAAGTAATCAAAATGGCTGATAACTCAACATTTTGCTCCAGATGTCAAG
GAGAAGGCGGAGAATGCTACATTGCAAATTATGAGATATCATTCAAACAA
AATAATACACAGTGTGCCTGTCCATATCGAACGGCAACAAGTGCTACAAT
TGATAACTGGAAGGGATCAGTAATATGTTCAGTTCTGTTGGCAAACATTG
GTGAGTATTGCAATCAAAAAAGAGTTATTTGTGGATCAAGTAAAGCTGAA
TGTTCATATTCTGAGGCATTTGAGGCAGAAGTTTGTCAATGCCCTCCTAA
ACATAGCATTGTGAAAAAGAATTTTGGAGATTTCTATCAGTGCCAGCCCA
ATATTCATGTTCAGACTGCTTGTAACAATTGTCAAATGTTAACTCAAGTT
TCAACGGAATCCCCTGAATCTATTCTTTTCACAGTATCCTGCACAAGTTA
CCAAATACGGTTATCATTAGATGTGGACCAAAAGTTACTTTTATCAGATG
AGTCCATTCTTTTTGTTTTCGGAACCCGGCCCGGAAATAATTACGAATTT
TTGAATTGTAAATTCAAAAAGCCCTCCAGCCTCCACAACCGGTATTATTG
GAGTATTGAAATTGACAAGGCAGCAAAACAAATGCCATGTGGAATTTATC
GAGTATTTTATCTGAACGGTGCTCTTTTTACATTCTATGTTGCCATATGG
AATAAACAGGATCGAGTTGATCTGATCAAAGATCACTTATACGAACTCTC
GTGTAGACTTTTCGAAAAGTCCATGGACATTGAGTCAGAAATCATTTCAA
GCCAGATTCTTGATCGATCATGGATAAATTATGGGGACCAAAGTGCAAAT
TTTTACAGTCTTAATCGAAAAAAGAAAGGCAAAATTAATTTTCAAAATTC
ACTAAATCTGTATCGAAATCAGTTTCAAAAGGAAGAATCCATCCATATCA
TTATCCGTTTTGATGAAGAGTACATGTCAAGAAGAAACAGAAAATATAAT
TATCTGGACGTGGAATCTTGCTCAGTAATTGATGAGAAATATGAGAAAAA
CACATCTCCTGTCGTTATGATTGAATTGTTCAACAAGGACTTCGTGACAA
TCAGTGACAAAAACTACCAATACAAAAGAAGCCATTTAATATCATTCAAT
TTGTCCAATCAGAATTCAGTAAATAGAAATTCAATTATTATAAAATGTAT
TGTGAGGCTGTGTGAAAAAGAATTATGGTGCAGTGCGAATTCCAATGTAC
TTCTTCCGAAACCGAATGTTGAATTCGAAGTACTGAAAAAGAAACTCATT
ATAATCAACACAATCCAGAGTACTCGAGTGGAATTTGTCGACGTTCATAA
AGAGCAATTTGATTCTCAAAAATGCCCATCATTTGTTTGTCTGACAAATG
TCCAAGTCTTGTTAATTATTTTCATTACATCACTAATCAGTATTTTCTTC
ATCGTTTCTCTCAGTATTCTTCTGGCTCAGATATATAAGACAAAACAGTC
AGATCTTTCACATCAGATCTCAAAAAATCCCATATCAGATTACATAGATC
TCAAATATTTACCTGACAGTAATAATTTGATTTTAAATGATGAAAATATT
CATCAAACTCCACTCAAAGACATTCGCTACCACAGCGAAGAATCTCTTGA
CAATTTACATCAGACCAATCAGGTCAATTTATTTAACATCATAGCAGCTA
CAATTAACCCCCCTTCCACCGATCATCGTTCTACAGAAATTTATGTCTAA
TTATTTTTTGTTTTATTTAATATTATATATGAAAATT
back to top

protein sequence of SMED30000648-orf-1

>SMED30000648-orf-1 ID=SMED30000648-orf-1|Name=SMED30000648-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=700bp
GFFILLQICQISSNSLTDEFRVVYDGNCINQGGNRFCRNKVLNSFCDNLE
NICLCLPGYVAIQESMGKLCQPVLGNSRCQIDSHCSNLTNKSTCHPGVGL
CICPADTMFVLQTNACEKVIKMADNSTFCSRCQGEGGECYIANYEISFKQ
NNTQCACPYRTATSATIDNWKGSVICSVLLANIGEYCNQKRVICGSSKAE
CSYSEAFEAEVCQCPPKHSIVKKNFGDFYQCQPNIHVQTACNNCQMLTQV
STESPESILFTVSCTSYQIRLSLDVDQKLLLSDESILFVFGTRPGNNYEF
LNCKFKKPSSLHNRYYWSIEIDKAAKQMPCGIYRVFYLNGALFTFYVAIW
NKQDRVDLIKDHLYELSCRLFEKSMDIESEIISSQILDRSWINYGDQSAN
FYSLNRKKKGKINFQNSLNLYRNQFQKEESIHIIIRFDEEYMSRRNRKYN
YLDVESCSVIDEKYEKNTSPVVMIELFNKDFVTISDKNYQYKRSHLISFN
LSNQNSVNRNSIIIKCIVRLCEKELWCSANSNVLLPKPNVEFEVLKKKLI
IINTIQSTRVEFVDVHKEQFDSQKCPSFVCLTNVQVLLIIFITSLISIFF
IVSLSILLAQIYKTKQSDLSHQISKNPISDYIDLKYLPDSNNLILNDENI
HQTPLKDIRYHSEESLDNLHQTNQVNLFNIIAATINPPSTDHRSTEIYV*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableTMHMMTMhelixcoord: 586..608