Cell wall integrity and stress response component 1

Overview
NameCell wall integrity and stress response component 1
Smed IDSMED30000647
Length (bp)943
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Cell wall integrity and stress response component 1 (SMED30000647) t-SNE clustered cells

Violin plots show distribution of expression levels for Cell wall integrity and stress response component 1 (SMED30000647) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Cell wall integrity and stress response component 1 (SMED30000647) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Cell wall integrity and stress response component 1 (SMED30000647) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30000647

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 10

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30000647SMESG000035037.1 SMESG000035036.1 dd_Smed_v4_6380_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30000647SMESG000035037.1 SMESG000035036.1 dd_Smed_v4_6380_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30000647SMESG000035037.1 SMESG000035036.1 dd_Smed_v4_6380_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30000647SMESG000035037.1 SMESG000035036.1 dd_Smed_v4_6380_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30000647SMESG000035038.1 SMESG000035037.1 SMESG000035036.1 Contig14607newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30000647SMESG000035038.1 SMESG000035037.1 SMESG000035036.1 Contig14607uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30000647SMESG000035037.1 SMESG000035036.1 OX_Smed_1.0.23230ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
Category 4 cellSMED30000647SMESG000035037.1 SMESG000035036.1 dd_Smed_v4_6380_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
epidermisSMED30000647SMESG000035037.1 SMESG000035036.1 dd_Smed_v4_6380_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
ciliated epithelial cellSMED30000647SMESG000035037.1 SMESG000035036.1 dd_Smed_v4_6380_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Cell wall integrity and stress response component 1 vs. TrEMBL
Match: A0A5P8I4L8 (Uncharacterized protein OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 532.332 bits (1370), Expect = 0.000e+0
Identity = 255/256 (99.61%), Postives = 255/256 (99.61%), Query Frame = 1
Query:   79 MNHRKNEILVIGAGQMRTGTFSLKFALEKLYNRPCYHMAEILNHHKDHVKLWISVFDSGKMNEKTAGKIFSDYSATVDFPSAAVYKDLMEIYPNAKVVLTTRNSDDWVKSVKSTTLRNNMKTSFVHKILFYFNLGTEFPVMLDKMYLKAYKEPFDALRTDDELLKQCYEEFNEEVRKYVPKERLLEFNAKEGWEPLCRFLNLPVPNESYPFVNTTKDFQRNVRGMMLKSVIRSGLVLSLTIGCLSLIIKHIYDKYF 846
            MNHRKNEILVIGAGQMRTGTFSLKFALEKLYNRPCYHMAEILNHHKDHVKLWISVFDSGKMNEKTAGKIFSDYSATVDFPSAAVYKDLMEIYPNAKVVLTTRNSDDWVKSVKSTTLRNNMKTSFVHKILFYFNLGTEFPVMLDKMYLKAYKEPFDALRTDDELLKQCYEEFNEEVRKYVPKERLLEFNAKEGWEPLCRFLNLPVPNE YPFVNTTKDFQRNVRGMMLKSVIRSGLVLSLTIGCLSLIIKHIYDKYF
Sbjct:    1 MNHRKNEILVIGAGQMRTGTFSLKFALEKLYNRPCYHMAEILNHHKDHVKLWISVFDSGKMNEKTAGKIFSDYSATVDFPSAAVYKDLMEIYPNAKVVLTTRNSDDWVKSVKSTTLRNNMKTSFVHKILFYFNLGTEFPVMLDKMYLKAYKEPFDALRTDDELLKQCYEEFNEEVRKYVPKERLLEFNAKEGWEPLCRFLNLPVPNEPYPFVNTTKDFQRNVRGMMLKSVIRSGLVLSLTIGCLSLIIKHIYDKYF 256          
BLAST of Cell wall integrity and stress response component 1 vs. TrEMBL
Match: A0A183LY04 (Uncharacterized protein OS=Schistosoma margrebowiei OX=48269 PE=4 SV=1)

HSP 1 Score: 197.978 bits (502), Expect = 7.819e-58
Identity = 100/231 (43.29%), Postives = 153/231 (66.23%), Query Frame = 1
Query:   94 NEILVIGAGQMRTGTFSLKFALEKLYNRPCYHMAEIL-NHHKDHVKLWISVFDSGK----MNEKTAGKIFSDYSATVDFPSAAVYKDLMEIYPNAKVVLTTRNSDDWVKSVKSTTLRNNMKT---SFVHKILFYFNLGTEFPVMLDKMYLKAYKEPFDALRTDDELLKQCYEEFNEEVRKYVPKERLLEFNAKEGWEPLCRFLNLPVPNESYPFVNTTKDFQRNVRGMMLK 762
            NE+L+IGAGQMRTGT SLKFAL+ L+N+PCYHM +++  + + H+K WI +F+  +    + +     IF++    VD+P+   YKDLM IYPNAKV+LT R++D W+ S ++TT  + + T   +F   I++        P++ D+MY KA+   +D + TDDE LK  + ++N+EV  YVPK+RLL F+  +GW+PLC FLN+P+P E+ PF +  K  + ++R  +LK
Sbjct:   50 NELLIIGAGQMRTGTTSLKFALQLLFNQPCYHMYDVIYKYQESHIKKWIKIFNMHQKCVNIEKAIWNDIFNECKFAVDYPTCVFYKDLMNIYPNAKVILTIRDTDSWISSCRATTASDMVMTKHITFTENIIYRLRGLRSLPILHDRMYTKAFGSNYDQM-TDDE-LKNAFIKWNQEVINYVPKDRLLIFDPNDGWKPLCEFLNIPIP-ENVPFPHLNK--RIDLRNRLLK 275          
BLAST of Cell wall integrity and stress response component 1 vs. TrEMBL
Match: A0A3P7ZFD7 (Uncharacterized protein OS=Schistosoma margrebowiei OX=48269 GN=SMRZ_LOCUS8679 PE=4 SV=1)

HSP 1 Score: 197.593 bits (501), Expect = 8.523e-58
Identity = 100/231 (43.29%), Postives = 153/231 (66.23%), Query Frame = 1
Query:   94 NEILVIGAGQMRTGTFSLKFALEKLYNRPCYHMAEIL-NHHKDHVKLWISVFDSGK----MNEKTAGKIFSDYSATVDFPSAAVYKDLMEIYPNAKVVLTTRNSDDWVKSVKSTTLRNNMKT---SFVHKILFYFNLGTEFPVMLDKMYLKAYKEPFDALRTDDELLKQCYEEFNEEVRKYVPKERLLEFNAKEGWEPLCRFLNLPVPNESYPFVNTTKDFQRNVRGMMLK 762
            NE+L+IGAGQMRTGT SLKFAL+ L+N+PCYHM +++  + + H+K WI +F+  +    + +     IF++    VD+P+   YKDLM IYPNAKV+LT R++D W+ S ++TT  + + T   +F   I++        P++ D+MY KA+   +D + TDDE LK  + ++N+EV  YVPK+RLL F+  +GW+PLC FLN+P+P E+ PF +  K  + ++R  +LK
Sbjct:   50 NELLIIGAGQMRTGTTSLKFALQLLFNQPCYHMYDVIYKYQESHIKKWIKIFNMHQKCVNIEKAIWNDIFNECKFAVDYPTCVFYKDLMNIYPNAKVILTIRDTDSWISSCRATTASDMVMTKHITFTENIIYRLRGLRSLPILHDRMYTKAFGSNYDQM-TDDE-LKNAFIKWNQEVINYVPKDRLLIFDPNDGWKPLCEFLNIPIP-ENVPFPHLNK--RIDLRNRLLK 275          
BLAST of Cell wall integrity and stress response component 1 vs. TrEMBL
Match: A0A183JZJ8 (Uncharacterized protein OS=Schistosoma curassoni OX=6186 GN=SCUD_LOCUS8156 PE=4 SV=1)

HSP 1 Score: 196.438 bits (498), Expect = 1.013e-56
Identity = 95/226 (42.04%), Postives = 151/226 (66.81%), Query Frame = 1
Query:   94 NEILVIGAGQMRTGTFSLKFALEKLYNRPCYHMAEIL-NHHKDHVKLWISVFDSGK----MNEKTAGKIFSDYSATVDFPSAAVYKDLMEIYPNAKVVLTTRNSDDWVKSVKSTTLRNNMKT---SFVHKILFYFNLGTEFPVMLDKMYLKAYKEPFDALRTDDELLKQCYEEFNEEVRKYVPKERLLEFNAKEGWEPLCRFLNLPVP-NESYPFVNTTKDFQRNV 744
            NE+L+IGAGQMRTGT SLKFAL+ L+N+PCYHM +++  + + H+K WI++F+  +    +++     IF++    +D+P+   YK+LM IYPNAKV+LT R++D W+ S ++TT  + + T   +F   I++        P++ DKMY KA+   +D + TDDE LK  + ++N+E+  YVPK+RLL F+  +GW+PLC FLN+P+P N  +P +N   D + ++
Sbjct:   98 NELLIIGAGQMRTGTTSLKFALQLLFNQPCYHMYDVIYKYQESHIKKWINIFNMHQKCVNIDKANWNDIFNECKFAMDYPTCVFYKELMNIYPNAKVILTIRDADSWISSCRATTASDMVMTKHITFTENIIYRLRGLKSLPILHDKMYTKAFGSNYDQM-TDDE-LKNAFIKWNQEIINYVPKDRLLIFDPNDGWKPLCEFLNIPIPENVPFPHLNKRIDLRNSL 321          
BLAST of Cell wall integrity and stress response component 1 vs. TrEMBL
Match: A0A267EKC2 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig019766g3 PE=4 SV=1)

HSP 1 Score: 193.356 bits (490), Expect = 1.513e-56
Identity = 95/256 (37.11%), Postives = 155/256 (60.55%), Query Frame = 1
Query:   46 RFSFCLNSFFTMNHRKNEILVIGAGQMRTGTFSLKFALEKLYNRPCYHMAEILNHH--KDHVKLWISVFDSGKMNEKTAGKIFSDYSATVDFPSAAVYKDLMEIYPNAKVVLTTRNSDDWVKSVKSTTLRN-NMKTSFVHKILFYFNLGTEFPVMLDKMYLKAYKEPFDALRTDDELLKQCYEEFNEEVRKYVPKERLLEFNAKEGWEPLCRFLNLPVPNESYPFVNTTKDFQRNVRGMMLKSVIRSGLVLSLTIG 804
            +  FC      M  + + ++VIGAG  RTGT S K ALE L  +PCYHM E L  H  + H+ +WI   +SG +++  A +I  +YSA VD+P+A+ YK+LM+ YPNAKV+L+ R+++ WV+S++S+ L+  +    F   + +   +G +   M+D ++ + + + ++    D E + + +EE N+ +R+ VP ERLLEF   +GW PLC+FLNLPVP++ +P +N  K  +R +  M   + +   L  +  IG
Sbjct:    1 QIQFCTKYQTKMPQKTHSLVVIGAGAPRTGTVSQKVALELLLGQPCYHMGEALGLHNSRRHIPMWIQAVESG-LSKSLADEILGNYSACVDYPTASFYKELMQHYPNAKVILSVRDTNSWVESMQSSILKAMHTMERFPRPLKWLLGIG-QADKMIDAVFRREFGDNYNL--ADSEFMARQFEERNDSIRRVVPSERLLEFRVSDGWAPLCQFLNLPVPDKPFPRLNDRKQVERALNIMTAITYVSDALATASCIG 252          
BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Nematostella
Match: EDO41210 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4T7])

HSP 1 Score: 94.7449 bits (234), Expect = 8.920e-23
Identity = 75/238 (31.51%), Postives = 119/238 (50.00%), Query Frame = 1
Query:  106 VIGAGQMRTGTFSLKFALEKLYNRPCYHMAEILNHHKDHVKLWISVFDSGKMNEKTAGKIFSDYSATVDFPSAAVYKDLMEIYPNAKVVLTTR-NSDDWVKSVKSTTLRNN--MKTSFVHKILFYFNL---GTEFPVMLDKMYLKAYKE--PFDALRTDDELLKQCYEEFNEEVRKYVPKERLLEFNAKEGWEPLCRFLNLPVPNESYPFVNTTKDFQRNVRGMMLKSVIRSGLVLSL 795
            VI AG  +TGT SL  AL ++     Y   E   H   H++ WI  F++ KM +    +++ D  A  D P    Y++L   +P AKV+L+ R N+D W +S+    L N   +K +   +++  F L   G +   M+  M   A+K   P  A+     L K+ Y E NE V+  +PK++LL F  ++GW PLCRFL +  P   +P+ N   +   +V+  +  S I + +   L
Sbjct:   25 VICAGMPKTGTKSLAKAL-RILGLKVYDADE---HCIYHMEEWIQAFETSKMPD--LYRMYKDVDAVTDVPPMFWYEELHYAFPEAKVILSVRDNTDVWYESL----LWNQKVLKEAMSLRVMLGFLLTPTGWKMSRMMSDMMNAAFKSTNPNAAI-----LNKKAYREHNERVKNVIPKDKLLVFKVEQGWPPLCRFLEIAEPEIFFPYENVNSE---DVKAFLHDSKIATKIYREL 244          
BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Nematostella
Match: EDO48575 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RIV5])

HSP 1 Score: 87.4261 bits (215), Expect = 2.450e-20
Identity = 68/249 (27.31%), Postives = 119/249 (47.79%), Query Frame = 1
Query:  106 VIGAGQMRTGTFSLKFALEKLYNRPCYHMAEILNHHKDHVKLWISVFDSGKMNEKTAGKIFSDYSATVDFPSAAVYKDLMEIYPNAKVVLTTRNSDD-WVKSVKSTTLRNNMKTSFVHKILFYFNLGTEFPVMLDKMYLKAYKEPFDALRTDDELL---KQCYEEFNEEVRKYVPKERLLEFNAKEGWEPLCRFLNLPVPNESYPFVNT-TKDFQRNVRGM-----MLKSVIRSGLVLSLTIGCLSLII 822
            +IGAG  +TGT SL  AL  L     Y++ +     + H   +I+ F+    N +   +++++  AT D P+   YK++   +P AKV+L+ R+ ++ W +S+  T   NN  +SF              P ++  M L      +  L  + ++    +  Y + N++V   +P ++LL FN K+GWEPLC FL   VP   YP  N  + D  R ++ +     M + +     V  + +GC   ++
Sbjct:    3 IIGAGLSKTGTKSLASALRHL----GYNVHDHQEQAQFHCDEYIAAFEGQMPNFE---EMYANVDATTDAPACFFYKEIFRAFPYAKVILSVRDDEESWRRSLMKT---NNFTSSFFKN-----------PFIILGMILTPSGRKYSRLGHNLKMYLNNELDYTKHNDDVLANIPAKQLLVFNPKDGWEPLCAFLGAEVPAIPYPRENVNSSDMPRIIKNIWHVRRMKRELAVMMAVFVVLLGCTCALL 230          
BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Nematostella
Match: EDO48573 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RIV3])

HSP 1 Score: 86.2705 bits (212), Expect = 6.331e-20
Identity = 67/215 (31.16%), Postives = 104/215 (48.37%), Query Frame = 1
Query:  106 VIGAGQMRTGTFSLKFALEKL----YNRPCYHMAEILNHHKDHVKLWISVFDSGKMNEKTAGKIFSDYSATVDFPSAAVYKDLMEIYPNAKVVLTTR-NSDDWVKSVKSTTLRNNMKTSFVHKILFYFNLGTEFPVMLDKMYLKAYKEPFDALRTDDELLKQC-----YEEFNEEVRKYVPKERLLEFNAKEGWEPLCRFLNLPVPNESYPFVNT 720
            +IGAG  +TGT SL  AL  L    ++RP     E +  H D    +I+ F+    N +   +++ +  AT D P+   YKD+   +P+AKV+L+ R N + W +S   T     + +S ++            P+++  M L      F   R +  L+K       Y++ N+ V   +  ++LL FN K+GWEPLC FL   VP   YP VN 
Sbjct:    3 IIGAGLPKTGTKSLASALRHLGYNVHDRP-----EQVQFHCDE---YIAAFEGQMPNFE---EMYENVDATTDVPACLFYKDIFRAFPDAKVILSVRDNEESWRRSFLKTL---EVLSSMINN-----------PLIILGMLLTPSGRKFSRFRHN--LIKYVENELDYKKHNDAVMANIQAKQLLVFNPKDGWEPLCAFLGAEVPTIPYPRVNV 190          
BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Nematostella
Match: EDO38503 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SCW7])

HSP 1 Score: 85.8853 bits (211), Expect = 7.209e-20
Identity = 69/233 (29.61%), Postives = 110/233 (47.21%), Query Frame = 1
Query:  109 IGAGQMRTGTFSLKFALEKLYNRPCYHMAEILNHHKDHVKLWISVFDSGKMNEKTAGKIFSDYSATVDFPSAAVYKDLMEIYPNAKVVLTTR-NSDDWVKSVKSTTLRNNMKTSFVHKILFYFNLGTEFPVMLDKMYLKAYKEPFDALRTDDELLKQC-YEEFNEEVRKYVPKERLLEFNAKEGWEPLCRFLNLPVPNESYPFVNTTKDFQRNVRGMMLKSVIRSGLVLSLTI 801
            I AG  +TGT SL  AL  L     Y + +I  + + H+  WI  F  G   + TA  +++     +D P     +D++   P  KV+LT R N+++W+             TS    +  ++++  +  + L ++     ++    +R     LK   Y    EEV+  VP +RLL FN KEGWEPLC FL    P+  +P  N   +F   +  M+ KS I + + + LTI
Sbjct:    4 ICAGLSKTGTKSLVAALRYL----GYTVYDIEENWRYHMDEWIKAFQ-GTNPDFTA--MYASVDTAIDLPPYLFVRDILVALPELKVILTVRENAEEWL-------------TSLTRSLDIWWSVYNQRWLKLARVLTPTGRKWATLIRHQTAALKTLDYTRHIEEVKAIVPADRLLVFNVKEGWEPLCAFLGHEAPSIPFPHRNKKSEF---IPDMINKSSIVARMRMELTI 213          
BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Nematostella
Match: EDO38865 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SBL0])

HSP 1 Score: 88.1965 bits (217), Expect = 1.050e-19
Identity = 66/229 (28.82%), Postives = 120/229 (52.40%), Query Frame = 1
Query:   94 NEIL-VIGAGQMRTGTFSLKFALEKLYNRPCYHMAEILNHHKDHVKLWISVFDSG-KMNEKTAGKIFSDYSATVDFPSAAVYKDLMEIYPNAKVVLTTR-NSDDWVKSV-KSTTLRNNMKTSFVHKILFYFN-LGTEFPVMLDKMYLKAYKEPFDALRTDDELLKQCYEEFNEEVRKYVPKERLLEFNAKEGWEPLCRFLNLPVPNESYPFVNTTKDFQRNVRGMMLKS 765
            NE++ VIGAG  +TGT SL  AL         H+   ++  ++H + +   +++  + N     K++ D  AT D P+   YK++ E +P++KV+L+ R N + W KS+ K+  + +++  S V K+  +    G E+  ++ ++ +    E       D       Y + N++V   +P  +LL FN +EGW PLC FL + VP+  +P +N +    ++V  ++L S
Sbjct:  189 NEVMKVIGAGLPKTGTKSLAAALR--------HLGCTVHDVEEHWRYYSDKYNAAFEGNIPDFKKMYKDVDATTDSPACFFYKEIFEAFPDSKVILSVRENEETWQKSLLKTKEVIDSVLDSPVIKLCQHVTPTGREWSRLVQRVGIHLQNE------VD-------YRKHNDDVIASIPTNQLLVFNPREGWVPLCAFLEVEVPDIPFPQINVS---SKDVSTILLNS 393          

HSP 2 Score: 79.7221 bits (195), Expect = 8.504e-17
Identity = 58/207 (28.02%), Postives = 101/207 (48.79%), Query Frame = 1
Query:  106 VIGAGQMRTGTFSLKFALEKLYNRPCYHMAEILNHHKDHVKLWISVFDSG-KMNEKTAGKIFSDYSATVDFPSAAVYKDLMEIYPNAKVVLTTR-NSDDWVKS-VKSTTLRNNMKTSFVHKILFYFNLGTEFPVMLDKMYLKAYKEPFDALRTDDELLKQCYEEFNEEVRKYVPKERLLEFNAKEGWEPLCRFLNLPVPNESYPFVN 717
            VIGAG  +TGT SL  AL         H+   ++  ++H + +   +++  + N     K++ D  AT D P+   YK++   +P++KV+L+ R N + W KS +K+  +  ++  S V K+  +        + L +      +   D            Y + N++V   +P  +LL FN +EGW PLC FL + VP+  +P +N
Sbjct:    3 VIGAGLPKTGTKSLAAALR--------HLGCTVHDVEEHWRFYCDEYNAAFEGNIPDFKKMYKDVDATTDSPACYFYKEIFNAFPDSKVILSVRENEETWKKSLIKTDEIMWSVLESPVVKLGQHVTPTGRKCIRLVQRVGIHLQNEVD------------YRKHNDDVIASIPANQLLVFNPREGWGPLCAFLEVKVPDIPFPQIN 189          
BLAST of Cell wall integrity and stress response component 1 vs. Planmine SMEST
Match: SMESG000035037.1 (SMESG000035037.1)

HSP 1 Score: 522.316 bits (1344), Expect = 0.000e+0
Identity = 251/256 (98.05%), Postives = 253/256 (98.83%), Query Frame = 1
Query:   79 MNHRKNEILVIGAGQMRTGTFSLKFALEKLYNRPCYHMAEILNHHKDHVKLWISVFDSGKMNEKTAGKIFSDYSATVDFPSAAVYKDLMEIYPNAKVVLTTRNSDDWVKSVKSTTLRNNMKTSFVHKILFYFNLGTEFPVMLDKMYLKAYKEPFDALRTDDELLKQCYEEFNEEVRKYVPKERLLEFNAKEGWEPLCRFLNLPVPNESYPFVNTTKDFQRNVRGMMLKSVIRSGLVLSLTIGCLSLIIKHIYDKYF 846
            MNHRKNEILVIGAGQMRTGTFSLKFALEKLYN PCYHMAEILNHHKDHVKLWISVFDSGKMNEKTAGKIFSDYSATVDFPSAAVYKDLMEIYPNAKVVLTTRNSDDWVKSVKSTTLRNNMKTSFVHKILFYFNLGTEFPVMLDKM+LKAYKEPFDALRTDDELLKQCYE+FNEEVRKYVPKERLLEFNAKEGWEPLCRFLNL VPNE YPFVNTTKDFQRNVRGMMLKSVIRSGLVLSLTIGCLSLIIKHIYDKYF
Sbjct:    1 MNHRKNEILVIGAGQMRTGTFSLKFALEKLYNCPCYHMAEILNHHKDHVKLWISVFDSGKMNEKTAGKIFSDYSATVDFPSAAVYKDLMEIYPNAKVVLTTRNSDDWVKSVKSTTLRNNMKTSFVHKILFYFNLGTEFPVMLDKMFLKAYKEPFDALRTDDELLKQCYEDFNEEVRKYVPKERLLEFNAKEGWEPLCRFLNLQVPNEPYPFVNTTKDFQRNVRGMMLKSVIRSGLVLSLTIGCLSLIIKHIYDKYF 256          
BLAST of Cell wall integrity and stress response component 1 vs. Planmine SMEST
Match: SMESG000035036.1 (SMESG000035036.1)

HSP 1 Score: 518.464 bits (1334), Expect = 0.000e+0
Identity = 247/256 (96.48%), Postives = 252/256 (98.44%), Query Frame = 1
Query:   79 MNHRKNEILVIGAGQMRTGTFSLKFALEKLYNRPCYHMAEILNHHKDHVKLWISVFDSGKMNEKTAGKIFSDYSATVDFPSAAVYKDLMEIYPNAKVVLTTRNSDDWVKSVKSTTLRNNMKTSFVHKILFYFNLGTEFPVMLDKMYLKAYKEPFDALRTDDELLKQCYEEFNEEVRKYVPKERLLEFNAKEGWEPLCRFLNLPVPNESYPFVNTTKDFQRNVRGMMLKSVIRSGLVLSLTIGCLSLIIKHIYDKYF 846
            MNHRKNE+LVIGAGQMRTGTFSLKFALEKLYN PCYHMAEILNHHKDHVKLWISVFDSGKMNEKTAGKIFSDYSATVDFPSAAVYKDLMEIYPNAKVVLTTRNSDDWVKSVKSTTLRNNMKTSFVHKILFYFNLGTEFPVMLDKMYLKAYKEPFDALRTDDELLKQCYE+FNEEVRKYVP+ERLLEFNAKEGWEPLCRFLNLPVPNE YPFVNTTKDFQ  +RGMMLKSVIRSGLVLSLTIGCLS+IIKHIYDKYF
Sbjct:    1 MNHRKNELLVIGAGQMRTGTFSLKFALEKLYNCPCYHMAEILNHHKDHVKLWISVFDSGKMNEKTAGKIFSDYSATVDFPSAAVYKDLMEIYPNAKVVLTTRNSDDWVKSVKSTTLRNNMKTSFVHKILFYFNLGTEFPVMLDKMYLKAYKEPFDALRTDDELLKQCYEDFNEEVRKYVPRERLLEFNAKEGWEPLCRFLNLPVPNEPYPFVNTTKDFQLTIRGMMLKSVIRSGLVLSLTIGCLSIIIKHIYDKYF 256          
BLAST of Cell wall integrity and stress response component 1 vs. Planmine SMEST
Match: SMESG000035096.1 (SMESG000035096.1)

HSP 1 Score: 490.73 bits (1262), Expect = 2.514e-177
Identity = 230/256 (89.84%), Postives = 245/256 (95.70%), Query Frame = 1
Query:   79 MNHRKNEILVIGAGQMRTGTFSLKFALEKLYNRPCYHMAEILNHHKDHVKLWISVFDSGKMNEKTAGKIFSDYSATVDFPSAAVYKDLMEIYPNAKVVLTTRNSDDWVKSVKSTTLRNNMKTSFVHKILFYFNLGTEFPVMLDKMYLKAYKEPFDALRTDDELLKQCYEEFNEEVRKYVPKERLLEFNAKEGWEPLCRFLNLPVPNESYPFVNTTKDFQRNVRGMMLKSVIRSGLVLSLTIGCLSLIIKHIYDKYF 846
            MNHRKNEILVIGAGQMRTGTFSLKFALEKLYNRPCYHMAEILNHHKDHVKLWISVFDSGKMNEKTAGKIFSDYSATVDFPSAAVYKDLMEIYPNAKVVLTTRNSDDWVKSV+STTL++ M+TS   K L +F++GTEFPVM DKM+LKAYK+PFDA+R DDELLKQCYE+FNEEVR YVPKERLLEFNAKEGWEPLCRFLNLPVPNE YPFVNTT+DFQRNVRG+MLKS+IRSGL +SLTIGCLSLIIKHIYDKYF
Sbjct:    1 MNHRKNEILVIGAGQMRTGTFSLKFALEKLYNRPCYHMAEILNHHKDHVKLWISVFDSGKMNEKTAGKIFSDYSATVDFPSAAVYKDLMEIYPNAKVVLTTRNSDDWVKSVRSTTLKDEMRTSLFDKFLLFFSIGTEFPVMCDKMFLKAYKKPFDAVRADDELLKQCYEDFNEEVRIYVPKERLLEFNAKEGWEPLCRFLNLPVPNEPYPFVNTTQDFQRNVRGIMLKSLIRSGLAISLTIGCLSLIIKHIYDKYF 256          
BLAST of Cell wall integrity and stress response component 1 vs. Planmine SMEST
Match: SMESG000035040.1 (SMESG000035040.1)

HSP 1 Score: 311.612 bits (797), Expect = 1.094e-106
Identity = 143/249 (57.43%), Postives = 188/249 (75.50%), Query Frame = 1
Query:   79 MNHRKNEILVIGAGQMRTGTFSLKFALEKLYNRPCYHMAEILNHHKDHVKLWISVFDSGKMNEKTAGKIFSDYSATVDFPSAAVYKDLMEIYPNAKVVLTTRNSDDWVKSVKSTTLRNNMKTSFVHKILFYFNLGTEFPVMLDKMYLKAYKEPFDALRTDDELLKQCYEEFNEEVRKYVPKERLLEFNAKEGWEPLCRFLNLPVPNESYPFVNTTKDFQRNVRGMMLKSVIRSGLVLSLTIGCLSLIIK 825
            M  + +E+ VIGAGQMRTGT SLK ALEKLYN  CYHMAEI  +H++H+ LWISVFD   +++ TA  + SDY ATVDFPS AVYKDLM++YPNAKV+LTTRNSDDW+KSV++TTL+     +F+ KI F F+    FP   +K+   AYK  ++ L  DD +LKQCYE+FN+EVR+ VPKERLLEFN+K+GWEPLC FLNLP+P+E YPFVN+T++F + +    L   ++ G+ +++  G +SLIIK
Sbjct:    1 MRKKNHELSVIGAGQMRTGTKSLKMALEKLYNGKCYHMAEIFENHRNHISLWISVFDKNGLDKSTAESLLSDYCATVDFPSTAVYKDLMKLYPNAKVILTTRNSDDWLKSVRNTTLKKGSDVTFLSKIFFMFSGLGRFPEFSEKLINHAYKNSYETLINDDAILKQCYEDFNQEVRETVPKERLLEFNSKQGWEPLCTFLNLPIPDEPYPFVNSTEEFNKYINKTYLADFVKYGIFVTIFAGAISLIIK 249          
BLAST of Cell wall integrity and stress response component 1 vs. Planmine SMEST
Match: SMESG000035067.1 (SMESG000035067.1)

HSP 1 Score: 249.98 bits (637), Expect = 2.022e-82
Identity = 121/247 (48.99%), Postives = 170/247 (68.83%), Query Frame = 1
Query:   79 MNHRKNEILVIGAGQMRTGTFSLKFALEKLYNRPCYHMAEILNHHKDHVKLWISVFDSGKMNEKTAGKIFSDYSATVDFPSAAVYKDLMEIYPNAKVVLTTRNSDDWVKSVKSTTLRNNMKTSFVHKILFYFNLGTEFPVMLDKMYLKAYKEPFDALRTDDELLKQCYEEFNEEVRKYVPKERLLEFNAKEGWEPLCRFLNLPVPNESYPFVNTTKDFQRNVRGMMLKSVIRSG-LVLSLTIGCLSL 816
            M    N++ VIGAGQMRTGT SLK ALEKLY    YHM EIL +  DH+ LWI   D G + ++ + +IF+ Y A VD P+A  Y+DL+EIYP+AKV+LTTRNSDDWVKSV+ST +R     + + KI  +      F  +  K+   ++  P++ L+ DDE+LK+ +E++NE VR+ VPKERLLEFNAK+GW PLC FL+LP+PNE +PFVN +++F   ++  + K++++ G + + LT G L L
Sbjct:    1 MTADSNKLFVIGAGQMRTGTTSLKSALEKLYGGKVYHMDEILFNGVDHIGLWIKALDQGHLCKELSEEIFTGYCAAVDNPTATFYQDLIEIYPDAKVILTTRNSDDWVKSVRSTIIRKGPIMNILEKISLWNQRIFRFEELCVKLRWHSFHVPYEILQYDDEVLKESFEKYNERVRQTVPKERLLEFNAKDGWTPLCEFLDLPIPNEPFPFVNNSEEFMDKIKSDITKTLVKFGSITVLLTFGILHL 247          
The following BLAST results are available for this feature:
BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5P8I4L80.000e+099.61Uncharacterized protein OS=Schmidtea mediterranea ... [more]
A0A183LY047.819e-5843.29Uncharacterized protein OS=Schistosoma margrebowie... [more]
A0A3P7ZFD78.523e-5843.29Uncharacterized protein OS=Schistosoma margrebowie... [more]
A0A183JZJ81.013e-5642.04Uncharacterized protein OS=Schistosoma curassoni O... [more]
A0A267EKC21.513e-5637.11Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO412108.920e-2331.51Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO485752.450e-2027.31Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO485736.331e-2031.16Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO385037.209e-2029.61Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO388651.050e-1928.82Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000035037.10.000e+098.05SMESG000035037.1[more]
SMESG000035036.10.000e+096.48SMESG000035036.1[more]
SMESG000035096.12.514e-17789.84SMESG000035096.1[more]
SMESG000035040.11.094e-10657.43SMESG000035040.1[more]
SMESG000035067.12.022e-8248.99SMESG000035067.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30000647 ID=SMED30000647|Name=Cell wall integrity and stress response component 1|organism=Schmidtea mediterranea sexual|type=transcript|length=943bp
TTTGTAATGAACTTTAGTAAATTATTTGAAATAATTTAAATTGAGCGGTT
CTCATTTTGTTTAAATTCATTTTTTACAATGAATCATAGAAAAAACGAGA
TTTTGGTTATTGGAGCTGGACAGATGAGAACCGGCACCTTTTCTTTGAAA
TTTGCTTTGGAAAAGCTTTATAATCGTCCTTGTTATCACATGGCGGAAAT
TCTGAATCACCATAAAGACCATGTCAAACTGTGGATTTCCGTTTTTGATT
CTGGAAAAATGAACGAAAAAACCGCTGGAAAAATATTTTCCGATTATTCT
GCGACTGTAGATTTCCCATCTGCCGCAGTCTATAAGGATCTGATGGAGAT
TTATCCCAATGCCAAAGTTGTACTAACAACTCGAAATTCCGATGATTGGG
TGAAGAGTGTCAAAAGTACTACTTTGAGGAATAACATGAAAACTAGTTTC
GTCCATAAGATTTTATTTTATTTCAATCTCGGAACTGAATTTCCAGTAAT
GTTAGACAAAATGTATCTTAAAGCTTATAAGGAACCATTTGATGCACTAA
GAACTGACGACGAGTTATTGAAGCAATGCTATGAAGAATTTAATGAGGAA
GTTCGTAAATATGTTCCCAAAGAAAGACTTTTGGAGTTCAACGCAAAAGA
AGGATGGGAACCATTGTGTCGCTTTTTGAATCTACCAGTTCCCAATGAGT
CATATCCATTTGTCAATACGACAAAGGATTTTCAACGGAACGTTAGAGGT
ATGATGCTGAAATCTGTTATTAGAAGCGGTCTTGTCCTTTCACTCACAAT
TGGGTGCTTGAGTCTAATAATAAAACATATTTATGATAAATATTTTTAAA
TTAAAAATTGAATAATTTGTGACATTTTAAACGTTAATATTTCGACAACT
AAATATGTTTATTTAATAAATAACAGTTTTGTTTAAAAAAAAA
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protein sequence of SMED30000647-orf-1

>SMED30000647-orf-1 ID=SMED30000647-orf-1|Name=SMED30000647-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=257bp
MNHRKNEILVIGAGQMRTGTFSLKFALEKLYNRPCYHMAEILNHHKDHVK
LWISVFDSGKMNEKTAGKIFSDYSATVDFPSAAVYKDLMEIYPNAKVVLT
TRNSDDWVKSVKSTTLRNNMKTSFVHKILFYFNLGTEFPVMLDKMYLKAY
KEPFDALRTDDELLKQCYEEFNEEVRKYVPKERLLEFNAKEGWEPLCRFL
NLPVPNESYPFVNTTKDFQRNVRGMMLKSVIRSGLVLSLTIGCLSLIIKH
IYDKYF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000018Category 4 cell
PLANA:0000026gut
PLANA:0000034epidermis
PLANA:0000106ciliated epithelial cell
PLANA:0000418head
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR040632Sulfotransfer_4
IPR027417P-loop_NTPase
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR040632Sulfotransferase, S. mansonii-typePFAMPF17784Sulfotransfer_4coord: 10..218
e-value: 7.0E-62
score: 209.1
NoneNo IPR availableGENE3DG3DSA:3.40.50.300coord: 1..238
e-value: 1.0E-68
score: 233.7
NoneNo IPR availablePANTHERPTHR36978FAMILY NOT NAMEDcoord: 4..247
NoneNo IPR availableTMHMMTMhelixcoord: 230..252
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILYSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 9..234