Microtubule-actin cross-linking factor 1-like isoform X28
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30000639 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 52Note: Hover over icons to view figure legend
Homology
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Human
Match: DST (dystonin [Source:HGNC Symbol;Acc:HGNC:1090]) HSP 1 Score: 262.692 bits (670), Expect = 4.411e-70 Identity = 190/681 (27.90%), Postives = 342/681 (50.22%), Query Frame = 3
Query: 4119 KIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIEN---FKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLK--LRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKS-LPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFK--LQNPSSFH---------KAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYRFGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRI 6110
K+ +RL ER R + A + K F ++++ L WL+ES +++ + P K+ L + K K L +++ + T R G LK+K ++ D + L +ML LR KW+ I V ++ L L+ +G DA++ + W+ + D + GD+D V LID + E+ + LK R L E + S ++ ++E+ ++ + L+ +L+ A+EF+S ++ L WL +AE+ L+ LP +E L LI++H+ +K+++E + + +G +L CHP + + I +R+ ++ + ++ ++L +L L L+E L+ + + ++ + IP +++ V +I +H F+ E++ + + K +K +PSS +A KR+ Y + T + N R+N L +++ ++W L++E + L + L L E + FDF+ W+K ++ ++ K R+ D FRR + G +EF + I+ S FPT++ + V +IF+++ + I Y +F+ +L P + + K I DK I D + ++VA C C R+ V++ GDNKYRFG + +L +VR L STVMVR+GGGW + EFL KNDPCR+
Sbjct: 470 KVVQRLVERGRSLDDARKRAKQFHEAWSKLMEWLEESEKSLDSELEIANDPDKIKTQLAQHKEFQKSLGAKHSVYDTTNRTGRSLKEKTSLAD-DNLKLDDMLSELRDKWDTICGKSVERQNKLEEALLFSGQFTDALQALIDWLYRVEPQLAEDQPVHGDIDLVMNLIDNHKAFQKELGKRTSSVQALKRSARELIEGSRDDSSWVKVQMQELSTRWETVCALSISKQTRLEAALRQAEEFHSVVHALLEWLAEAEQTLRFHGVLP--DDEDALRTLIDQHKEFMKKLEEKRAELNKATTMGDTVLAICHPDSITTIKHWITIIRARFEEVLAWAKQHQQRLASALAGLIAKQELLEALLAWLQWAETTLTDKDKEVIPQEIEEVKALIAEHQTFMEEMTRKQPDVDKVTKTYKRRAADPSSLQSHIPVLDKGRAGRKRF-PASSLYPS---------GSQTQIETKNPRVNLLVSKWQQVWLLALERRRKLNDALDRLEELREFANFDFDIWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILSSKFPTSRLEMSAVADIFDRDGDGYIDYYEFVAALHPNKDAY--KPITDADK------IEDEVTRQVAKCKCAKRFQVEQIGDNKYRFGDSQQLRLVRILRSTVMVRVGGGWMALDEFLVKNDPCRV 1129 HSP 2 Score: 63.5438 bits (153), Expect = 5.694e-9 Identity = 56/262 (21.37%), Postives = 112/262 (42.75%), Query Frame = 3
Query: 4368 DKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHK---EDFKNLKLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEK-LLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESL 5141
DK TV+DP L L+ W+ + I+ + L L+ G A+ + W+ + + GD +E+ + + H+ +N++ H+ E ++ E S+++N +E + ++ + +QR + L +L+ AK F+ ++ +L WL E+ LL K L A E + H + + + T + G+ +L C A +++ I +W + ++ +R KL E+L
Sbjct: 126 DKHTVQDP--------LMELKLIWDSLEERIINRQHKLEGALLALGQFQHALDELLAWLTHTEGLLSEQKPVGGDPKAIEIELAKHHVLQNDVLAHQSTVEAVNKAGNDLIESSAGEEASNLQNKLEVLNQRWQNVLEKTEQRKQQLDGALRQAKGFHGEIEDLQQWLTDTERHLLASKPLGGLPETAK--EQLNVHMEVCAAFEAKEETYKSLMQKGQQMLARCPKSAETNIDQDINNLKEKWESVETKLNERKTKLEEAL 377
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Human
Match: DST (dystonin [Source:HGNC Symbol;Acc:HGNC:1090]) HSP 1 Score: 257.684 bits (657), Expect = 9.167e-68 Identity = 199/753 (26.43%), Postives = 360/753 (47.81%), Query Frame = 3
Query: 4119 KIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIEN---FKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLK--LRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKS-LPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFKLQ--NPSSFH---------KAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYR------FGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRIANSLDLQ--------KSKSMKMSFSXXXXXXXXXXXXDEKPTVKKSIQYQKASSLSSRIPTGLSKK 6284
K+ +RL ER R + A + K F ++++ L WL+ES +++ + P K+ L + K K L +++ + T R G LK+K ++ D + L +ML LR KW+ I V ++ L L+ +G DA++ + W+ + D + GD+D V LID + E+ + LK R L E + S ++ ++E+ ++ + L+ +L+ A+EF+S ++ L WL +AE+ L+ LP +E L LI++H+ +K+++E + + +G +L CHP + + I +R+ ++ + ++ ++L +L L L+E L+ + + ++ + IP +++ V +I +H F+ E++ + + K +K + +PSS +A KR+ Y + T + N R+N L +++ ++W L++E + L + L L E + FDF+ W+K ++ ++ K R+ D FRR + G +EF + I+ S FPT++ + V +IF+++ + I Y +F+ +L P + + K I DK I D + ++VA C C R+ V++ GDNKYR FG + +L +VR L STVMVR+GGGW + EFL KNDPCR +++ S M+ P S+ Q A + S ++P + K
Sbjct: 4601 KVVQRLVERGRSLDDARKRAKQFHEAWSKLMEWLEESEKSLDSELEIANDPDKIKTQLAQHKEFQKSLGAKHSVYDTTNRTGRSLKEKTSLAD-DNLKLDDMLSELRDKWDTICGKSVERQNKLEEALLFSGQFTDALQALIDWLYRVEPQLAEDQPVHGDIDLVMNLIDNHKAFQKELGKRTSSVQALKRSARELIEGSRDDSSWVKVQMQELSTRWETVCALSISKQTRLEAALRQAEEFHSVVHALLEWLAEAEQTLRFHGVLP--DDEDALRTLIDQHKEFMKKLEEKRAELNKATTMGDTVLAICHPDSITTIKHWITIIRARFEEVLAWAKQHQQRLASALAGLIAKQELLEALLAWLQWAETTLTDKDKEVIPQEIEEVKALIAEHQTFMEEMTRKQPDVDKVTKTYKRRAADPSSLQSHIPVLDKGRAGRKRF-PASSLYPS---------GSQTQIETKNPRVNLLVSKWQQVWLLALERRRKLNDALDRLEELREFANFDFDIWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILSSKFPTSRLEMSAVADIFDRDGDGYIDYYEFVAALHPNKDAY--KPITDADK------IEDEVTRQVAKCKCAKRFQVEQIGDNKYRFFLGNQFGDSQQLRLVRILRSTVMVRVGGGWMALDEFLVKNDPCRAKGRTNMELREKFILADGASQGMAAFRPRGRRSRPSSRGASPNRSTSVSSQAAQAASPQVPATTTPK 5332 HSP 2 Score: 63.1586 bits (152), Expect = 7.501e-9 Identity = 56/262 (21.37%), Postives = 112/262 (42.75%), Query Frame = 3
Query: 4368 DKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHK---EDFKNLKLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEK-LLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESL 5141
DK TV+DP L L+ W+ + I+ + L L+ G A+ + W+ + + GD +E+ + + H+ +N++ H+ E ++ E S+++N +E + ++ + +QR + L +L+ AK F+ ++ +L WL E+ LL K L A E + H + + + T + G+ +L C A +++ I +W + ++ +R KL E+L
Sbjct: 4257 DKHTVQDP--------LMELKLIWDSLEERIINRQHKLEGALLALGQFQHALDELLAWLTHTEGLLSEQKPVGGDPKAIEIELAKHHVLQNDVLAHQSTVEAVNKAGNDLIESSAGEEASNLQNKLEVLNQRWQNVLEKTEQRKQQLDGALRQAKGFHGEIEDLQQWLTDTERHLLASKPLGGLPETAK--EQLNVHMEVCAAFEAKEETYKSLMQKGQQMLARCPKSAETNIDQDINNLKEKWESVETKLNERKTKLEEAL 4508
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Human
Match: DST (dystonin [Source:HGNC Symbol;Acc:HGNC:1090]) HSP 1 Score: 257.299 bits (656), Expect = 1.240e-67 Identity = 199/753 (26.43%), Postives = 360/753 (47.81%), Query Frame = 3
Query: 4119 KIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIEN---FKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLK--LRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKS-LPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFKLQ--NPSSFH---------KAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYR------FGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRIANSLDLQ--------KSKSMKMSFSXXXXXXXXXXXXDEKPTVKKSIQYQKASSLSSRIPTGLSKK 6284
K+ +RL ER R + A + K F ++++ L WL+ES +++ + P K+ L + K K L +++ + T R G LK+K ++ D + L +ML LR KW+ I V ++ L L+ +G DA++ + W+ + D + GD+D V LID + E+ + LK R L E + S ++ ++E+ ++ + L+ +L+ A+EF+S ++ L WL +AE+ L+ LP +E L LI++H+ +K+++E + + +G +L CHP + + I +R+ ++ + ++ ++L +L L L+E L+ + + ++ + IP +++ V +I +H F+ E++ + + K +K + +PSS +A KR+ Y + T + N R+N L +++ ++W L++E + L + L L E + FDF+ W+K ++ ++ K R+ D FRR + G +EF + I+ S FPT++ + V +IF+++ + I Y +F+ +L P + + K I DK I D + ++VA C C R+ V++ GDNKYR FG + +L +VR L STVMVR+GGGW + EFL KNDPCR +++ S M+ P S+ Q A + S ++P + K
Sbjct: 4275 KVVQRLVERGRSLDDARKRAKQFHEAWSKLMEWLEESEKSLDSELEIANDPDKIKTQLAQHKEFQKSLGAKHSVYDTTNRTGRSLKEKTSLAD-DNLKLDDMLSELRDKWDTICGKSVERQNKLEEALLFSGQFTDALQALIDWLYRVEPQLAEDQPVHGDIDLVMNLIDNHKAFQKELGKRTSSVQALKRSARELIEGSRDDSSWVKVQMQELSTRWETVCALSISKQTRLEAALRQAEEFHSVVHALLEWLAEAEQTLRFHGVLP--DDEDALRTLIDQHKEFMKKLEEKRAELNKATTMGDTVLAICHPDSITTIKHWITIIRARFEEVLAWAKQHQQRLASALAGLIAKQELLEALLAWLQWAETTLTDKDKEVIPQEIEEVKALIAEHQTFMEEMTRKQPDVDKVTKTYKRRAADPSSLQSHIPVLDKGRAGRKRF-PASSLYPS---------GSQTQIETKNPRVNLLVSKWQQVWLLALERRRKLNDALDRLEELREFANFDFDIWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILSSKFPTSRLEMSAVADIFDRDGDGYIDYYEFVAALHPNKDAY--KPITDADK------IEDEVTRQVAKCKCAKRFQVEQIGDNKYRFFLGNQFGDSQQLRLVRILRSTVMVRVGGGWMALDEFLVKNDPCRAKGRTNMELREKFILADGASQGMAAFRPRGRRSRPSSRGASPNRSTSVSSQAAQAASPQVPATTTPK 5006 HSP 2 Score: 63.1586 bits (152), Expect = 9.378e-9 Identity = 56/262 (21.37%), Postives = 112/262 (42.75%), Query Frame = 3
Query: 4368 DKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHK---EDFKNLKLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEK-LLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESL 5141
DK TV+DP L L+ W+ + I+ + L L+ G A+ + W+ + + GD +E+ + + H+ +N++ H+ E ++ E S+++N +E + ++ + +QR + L +L+ AK F+ ++ +L WL E+ LL K L A E + H + + + T + G+ +L C A +++ I +W + ++ +R KL E+L
Sbjct: 3931 DKHTVQDP--------LMELKLIWDSLEERIINRQHKLEGALLALGQFQHALDELLAWLTHTEGLLSEQKPVGGDPKAIEIELAKHHVLQNDVLAHQSTVEAVNKAGNDLIESSAGEEASNLQNKLEVLNQRWQNVLEKTEQRKQQLDGALRQAKGFHGEIEDLQQWLTDTERHLLASKPLGGLPETAK--EQLNVHMEVCAAFEAKEETYKSLMQKGQQMLARCPKSAETNIDQDINNLKEKWESVETKLNERKTKLEEAL 4182
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Human
Match: DST (dystonin [Source:HGNC Symbol;Acc:HGNC:1090]) HSP 1 Score: 256.529 bits (654), Expect = 1.375e-67 Identity = 190/687 (27.66%), Postives = 343/687 (49.93%), Query Frame = 3
Query: 4119 KIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIEN---FKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLK--LRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKS-LPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFKLQ--NPSSFH---------KAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYR------FGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRI 6110
K+ +RL ER R + A + K F ++++ L WL+ES +++ + P K+ L + K K L +++ + T R G LK+K ++ D + L +ML LR KW+ I V ++ L L+ +G DA++ + W+ + D + GD+D V LID + E+ + LK R L E + S ++ ++E+ ++ + L+ +L+ A+EF+S ++ L WL +AE+ L+ LP +E L LI++H+ +K+++E + + +G +L CHP + + I +R+ ++ + ++ ++L +L L L+E L+ + + ++ + IP +++ V +I +H F+ E++ + + K +K + +PSS +A KR+ Y + T + N R+N L +++ ++W L++E + L + L L E + FDF+ W+K ++ ++ K R+ D FRR + G +EF + I+ S FPT++ + V +IF+++ + I Y +F+ +L P + + K I DK I D + ++VA C C R+ V++ GDNKYR FG + +L +VR L STVMVR+GGGW + EFL KNDPCR+
Sbjct: 1793 KVVQRLVERGRSLDDARKRAKQFHEAWSKLMEWLEESEKSLDSELEIANDPDKIKTQLAQHKEFQKSLGAKHSVYDTTNRTGRSLKEKTSLAD-DNLKLDDMLSELRDKWDTICGKSVERQNKLEEALLFSGQFTDALQALIDWLYRVEPQLAEDQPVHGDIDLVMNLIDNHKAFQKELGKRTSSVQALKRSARELIEGSRDDSSWVKVQMQELSTRWETVCALSISKQTRLEAALRQAEEFHSVVHALLEWLAEAEQTLRFHGVLP--DDEDALRTLIDQHKEFMKKLEEKRAELNKATTMGDTVLAICHPDSITTIKHWITIIRARFEEVLAWAKQHQQRLASALAGLIAKQELLEALLAWLQWAETTLTDKDKEVIPQEIEEVKALIAEHQTFMEEMTRKQPDVDKVTKTYKRRAADPSSLQSHIPVLDKGRAGRKRF-PASSLYPS---------GSQTQIETKNPRVNLLVSKWQQVWLLALERRRKLNDALDRLEELREFANFDFDIWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILSSKFPTSRLEMSAVADIFDRDGDGYIDYYEFVAALHPNKDAY--KPITDADK------IEDEVTRQVAKCKCAKRFQVEQIGDNKYRFFLGNQFGDSQQLRLVRILRSTVMVRVGGGWMALDEFLVKNDPCRV 2458 HSP 2 Score: 63.5438 bits (153), Expect = 5.666e-9 Identity = 56/262 (21.37%), Postives = 112/262 (42.75%), Query Frame = 3
Query: 4368 DKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHK---EDFKNLKLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEK-LLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESL 5141
DK TV+DP L L+ W+ + I+ + L L+ G A+ + W+ + + GD +E+ + + H+ +N++ H+ E ++ E S+++N +E + ++ + +QR + L +L+ AK F+ ++ +L WL E+ LL K L A E + H + + + T + G+ +L C A +++ I +W + ++ +R KL E+L
Sbjct: 1449 DKHTVQDP--------LMELKLIWDSLEERIINRQHKLEGALLALGQFQHALDELLAWLTHTEGLLSEQKPVGGDPKAIEIELAKHHVLQNDVLAHQSTVEAVNKAGNDLIESSAGEEASNLQNKLEVLNQRWQNVLEKTEQRKQQLDGALRQAKGFHGEIEDLQQWLTDTERHLLASKPLGGLPETAK--EQLNVHMEVCAAFEAKEETYKSLMQKGQQMLARCPKSAETNIDQDINNLKEKWESVETKLNERKTKLEEAL 1700
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Human
Match: DST (dystonin [Source:HGNC Symbol;Acc:HGNC:1090]) HSP 1 Score: 257.299 bits (656), Expect = 1.435e-67 Identity = 199/753 (26.43%), Postives = 360/753 (47.81%), Query Frame = 3
Query: 4119 KIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIEN---FKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLK--LRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKS-LPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFKLQ--NPSSFH---------KAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYR------FGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRIANSLDLQ--------KSKSMKMSFSXXXXXXXXXXXXDEKPTVKKSIQYQKASSLSSRIPTGLSKK 6284
K+ +RL ER R + A + K F ++++ L WL+ES +++ + P K+ L + K K L +++ + T R G LK+K ++ D + L +ML LR KW+ I V ++ L L+ +G DA++ + W+ + D + GD+D V LID + E+ + LK R L E + S ++ ++E+ ++ + L+ +L+ A+EF+S ++ L WL +AE+ L+ LP +E L LI++H+ +K+++E + + +G +L CHP + + I +R+ ++ + ++ ++L +L L L+E L+ + + ++ + IP +++ V +I +H F+ E++ + + K +K + +PSS +A KR+ Y + T + N R+N L +++ ++W L++E + L + L L E + FDF+ W+K ++ ++ K R+ D FRR + G +EF + I+ S FPT++ + V +IF+++ + I Y +F+ +L P + + K I DK I D + ++VA C C R+ V++ GDNKYR FG + +L +VR L STVMVR+GGGW + EFL KNDPCR +++ S M+ P S+ Q A + S ++P + K
Sbjct: 4641 KVVQRLVERGRSLDDARKRAKQFHEAWSKLMEWLEESEKSLDSELEIANDPDKIKTQLAQHKEFQKSLGAKHSVYDTTNRTGRSLKEKTSLAD-DNLKLDDMLSELRDKWDTICGKSVERQNKLEEALLFSGQFTDALQALIDWLYRVEPQLAEDQPVHGDIDLVMNLIDNHKAFQKELGKRTSSVQALKRSARELIEGSRDDSSWVKVQMQELSTRWETVCALSISKQTRLEAALRQAEEFHSVVHALLEWLAEAEQTLRFHGVLP--DDEDALRTLIDQHKEFMKKLEEKRAELNKATTMGDTVLAICHPDSITTIKHWITIIRARFEEVLAWAKQHQQRLASALAGLIAKQELLEALLAWLQWAETTLTDKDKEVIPQEIEEVKALIAEHQTFMEEMTRKQPDVDKVTKTYKRRAADPSSLQSHIPVLDKGRAGRKRF-PASSLYPS---------GSQTQIETKNPRVNLLVSKWQQVWLLALERRRKLNDALDRLEELREFANFDFDIWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILSSKFPTSRLEMSAVADIFDRDGDGYIDYYEFVAALHPNKDAY--KPITDADK------IEDEVTRQVAKCKCAKRFQVEQIGDNKYRFFLGNQFGDSQQLRLVRILRSTVMVRVGGGWMALDEFLVKNDPCRAKGRTNMELREKFILADGASQGMAAFRPRGRRSRPSSRGASPNRSTSVSSQAAQAASPQVPATTTPK 5372 HSP 2 Score: 63.1586 bits (152), Expect = 8.874e-9 Identity = 56/262 (21.37%), Postives = 112/262 (42.75%), Query Frame = 3
Query: 4368 DKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHK---EDFKNLKLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEK-LLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESL 5141
DK TV+DP L L+ W+ + I+ + L L+ G A+ + W+ + + GD +E+ + + H+ +N++ H+ E ++ E S+++N +E + ++ + +QR + L +L+ AK F+ ++ +L WL E+ LL K L A E + H + + + T + G+ +L C A +++ I +W + ++ +R KL E+L
Sbjct: 4297 DKHTVQDP--------LMELKLIWDSLEERIINRQHKLEGALLALGQFQHALDELLAWLTHTEGLLSEQKPVGGDPKAIEIELAKHHVLQNDVLAHQSTVEAVNKAGNDLIESSAGEEASNLQNKLEVLNQRWQNVLEKTEQRKQQLDGALRQAKGFHGEIEDLQQWLTDTERHLLASKPLGGLPETAK--EQLNVHMEVCAAFEAKEETYKSLMQKGQQMLARCPKSAETNIDQDINNLKEKWESVETKLNERKTKLEEAL 4548
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Celegans
Match: vab-10 (VAB-10B protein [Source:UniProtKB/TrEMBL;Acc:G5EFM4]) HSP 1 Score: 162.54 bits (410), Expect = 2.823e-39 Identity = 277/1400 (19.79%), Postives = 548/1400 (39.14%), Query Frame = 3
Query: 2013 VQSFPEKFNKQFEEVSFLYDQVKKKEIAFHPLKQAINKLLLDDNMSCTQDDIKEQFDGVGIDNEAVLSNDADDLKDHLKEVECLIESVFYKVDNRMSILNELKDLVNLYWSEISEIFSSIKHIQKNAMSIEAFPYNKDDKNKILEPQIAHSDSISTLTDADNKSTTSSVGFELDEKFDEDSKLELPNLTESKFPEEIISQDLVKQQYSSLKKMGKDVTQFVEKLETDLVPKANQIVKIGGQFCELEISEFTESLLEEIGITEDAIEEKDILFNKALYFVDFLSDEIKKVFEWFEVMENRLINFGEITPDQKLIEINLL--ELKSLNTEIFSKAQQLDKIGSAISEILSYPYVPNKNTLEEMKLNIKEKYSELVKGIIEKHNDLQKKYSDIGELDFSLDDNIGILQEFENQIKNLTPQSCKGDPEYTFSCFKNLKFLDDEFNGLQSDFQNLCERISAHGDIEDNTNLRCRGTLSIFGKL--------KEMKILTEQIRVLIPEKYVMIQEILKNEKL-LSKEIHLHIDXXXXXXXXXXXXXXXGFSQESVEQSNLIFSNYIGDLNKQRNSIENSLKLASSHIESCQYDKCTFLSTAIKTLFHKYKRCQHLAAKRKEKLSIYSKLILEYDADKQTLQKDILELE-----NLSIKRMNPSKITVTCAEQIKSIKISIQTADKNFDIYEHLTIRSFDIKCFGNRIDNSQLILELKEYGLKLAKIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIE---NFKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEML-KTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKF--VPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNL--KLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDM----QNVNDIINKHLIFINELSEIDSQIKSLIKDFKLQNPSSFHKAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYRFGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRIANSLDL 6128
+ + P + E + + ++ K+ A+ LKQ ++LL S + D A D+++ L +E L + + + +R +L ++ D +WSE+ ++ ++ ++ +++EP H + + AD + + V E++ + + +L GK + +V E ++ NQ+ + G F + F E + I E+A+ F D ++++ +W ++ E +L+ + + + EI L EL + E+ + +++ + ++ + T+ + ++ ++S L + E+ + L+ +G+L ++ + + +K++ P KN L D Q L I AH D + N + + G L K M + E I+ ++ E E+ K E + E+ +LE+ + L G E+ E F D+ + +E L H++ D T+LS + K+ + + L R++KL + + + ++ ++ I+ E SI R+ P I AE ++ + A + + +H ++ + + D + L ++ KI R ER + E + D +L ++ + V I N K+ L+++K + ++ + F T + G L ++ T + Y + M + + KW ++ + + +++ G ++M + W++D + ++ + D+D V L+DE E + K + K L E+ I E + +++ + R S++E+ Q A+EF++K + L WL E+ LK L D VE +++ ++ E K G+ IL+ C P A + + +RW +++ + ++R L E + IE+L + ++ I D+ QN+ D N +DS+++ Q P D+ KS + N L+T++ KLW ++ + +L N L E ++L + + WK+ ++ + R+ DLFRR G + F + II S FPT++ + +V + F+K + I ++FI++LR + N N + I H+I E + C+C Y +++ +N YRFG MVR L STVMVR+GGGW+++ EFL K+DPCR L++
Sbjct: 3556 ISADPSVLESRLAENRSIVESLRDKQNAYDALKQTASELL----ASAPEGDAA-----------------AGDVENKLNRLEKLWKEIEREAVDRGVLLEDVLDKAKHFWSELDSCQKAVDDLR--------------NRLELVEPATGHPEQL-----ADQQEIMAQVASEMER---------------------------ARPRIEALSIAGKQLADYVPDDEKAVIE--NQVANVRGGFSTI-TGLFAEKKRDLIAAMEEAM--------------TFHGD-LQELLKWLDMAEQKLLKMSPVEHAKHMTEIEQLLKELHTFKDEVHERGVAKEQVVATALQLAADAPPHLAATVRQPVADLNTRWSRLNAALAEREHKLENLMLQMGKLASTIAQLTAWMDKTRATLKDIAPP-------------KNAVNLRD-IEIAQCKLVVLSNDIHAHQDSVNAVNRAAQKYIQTSGALDAETSDSLKSMNLKWEDIQKVL-ESLAFDMEVAKKEAENVGGEVEKWQRWLEETESALLSTKPTGGLPETAEFQLDEFKALKLDVEHNASPLEAHLHATEQHLKEEPQDADTWLSKTHGAMKTKWNKVKELLVDREKKLQVAYEQAVALESALNDMEDWIIAAERKLTDQPSISRL-PDVIEKQLAEHESWME---EVAGRKMAMTKH-QASGVHMQYYCEKKDAIPIKNRLVSLKHRVEKISGRTAERAKQLAVTRDEVATWQDGLHDLEHFISDVLVKIAPEPNTTSSLEKLKAKLEEVKEAQRDVTAKQTLFDVTRKRGIGLAERATRSE---YKQISMTNEKMSKKWAEMLKKLRDRLREAEQAVLEGGAFEESMNDLESWVDDELERYQKAEHEPVFADIDGVRALVDEESRRSAERKTKENGVKTVVKKADALMASGVDEKDSIAQAKERLVEKWNQVEEAARHRGNSIKEAEQAAEEFDAKTHALLDWLAVEEQKLKASGL-------DEVEGVKQ------EMDEAKGRYQECLKKGEEILSKCQPAAEPILRNWMRVVEARWKEVSEKVDEREFTLLEQEQKAKEQNEQIEKLAKFAAQKREELNRMIEQPPAQDLDTMEQNICDFAN-----------LDSELRE-------QQPEV------------------------DAACKSAKKGARNPAAEMLSTEWKKLWLDAMGLQSSLDNQKALLEEMKRLEGWKWEDWKERYVEWNDHAKARVNDLFRRIDRLHTGNVPRQVFIDGIIGSKFPTSRLEMAKVADRFDKGDGM-INAKEFINALRFDA-----SNRNAKPQTDTEKITHEI-ELQKKTCSCCTPYQIEKISENHYRFGDTHIKRMVRILRSTVMVRVGGGWESLDEFLHKHDPCRAKGRLNI 4785
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Celegans
Match: vab-10 (VAB-10B protein [Source:UniProtKB/TrEMBL;Acc:G5EFM4]) HSP 1 Score: 162.155 bits (409), Expect = 3.341e-39 Identity = 276/1400 (19.71%), Postives = 547/1400 (39.07%), Query Frame = 3
Query: 2013 VQSFPEKFNKQFEEVSFLYDQVKKKEIAFHPLKQAINKLLLDDNMSCTQDDIKEQFDGVGIDNEAVLSNDADDLKDHLKEVECLIESVFYKVDNRMSILNELKDLVNLYWSEISEIFSSIKHIQKNAMSIEAFPYNKDDKNKILEPQIAHSDSISTLTDADNKSTTSSVGFELDEKFDEDSKLELPNLTESKFPEEIISQDLVKQQYSSLKKMGKDVTQFVEKLETDLVPKANQIVKIGGQFCELEISEFTESLLEEIGITEDAIEEKDILFNKALYFVDFLSDEIKKVFEWFEVMENRLINFGEITPDQKLIEINLL--ELKSLNTEIFSKAQQLDKIGSAISEILSYPYVPNKNTLEEMKLNIKEKYSELVKGIIEKHNDLQKKYSDIGELDFSLDDNIGILQEFENQIKNLTPQSCKGDPEYTFSCFKNLKFLDDEFNGLQSDFQNLCERISAHGDIEDNTNLRCRGTLSIFGKL--------KEMKILTEQIRVLIPEKYVMIQEILKNEKL-LSKEIHLHIDXXXXXXXXXXXXXXXGFSQESVEQSNLIFSNYIGDLNKQRNSIENSLKLASSHIESCQYDKCTFLSTAIKTLFHKYKRCQHLAAKRKEKLSIYSKLILEYDADKQTLQKDILELE-----NLSIKRMNPSKITVTCAEQIKSIKISIQTADKNFDIYEHLTIRSFDIKCFGNRIDNSQLILELKEYGLKLAKIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIE---NFKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEML-KTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKF--VPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNL--KLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDM----QNVNDIINKHLIFINELSEIDSQIKSLIKDFKLQNPSSFHKAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYRFGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRIANSLDL 6128
+ + P + E + + ++ K+ A+ LKQ ++LL S + D A D+++ L +E L + + + +R +L ++ D +WSE+ ++ ++ ++ +++EP H + + AD + + V E++ + + +L GK + +V E ++ NQ+ + G F + F E + I E+A+ F D ++++ +W ++ E +L+ + + + EI L EL + E+ + +++ + ++ + T+ + ++ ++S L + E+ + L+ +G+L ++ + + +K++ P KN L D Q L I AH D + N + + G L K M + E I+ ++ E E+ K E + E+ +LE+ + L G E+ E F D+ + +E L H++ D T+LS + K+ + + L R++KL + + + ++ ++ I+ E SI R+ P I AE ++ + A + + +H ++ + + D + L ++ KI R ER + E + D +L ++ + V I N K+ L+++K + ++ + F T + G L ++ T + Y + M + + KW ++ + + +++ G ++M + W++D + ++ + D+D V L+DE E + K + K L E+ I E + +++ + R S++E+ Q A+EF++K + L WL E+ LK L D VE +++ ++ E K G+ IL+ C P A + + +RW +++ + ++R L E + IE+L + ++ I D+ QN+ D N + E+D+ KS K + NP++ L+T++ KLW ++ + +L N L E ++L + + WK+ ++ + R+ DLFRR G + F + II S FPT++ + +V + F+K + I ++FI++LR + N N + I H+I E + C+C Y +++ +N YRFG MVR L STVMVR+GGGW+++ EFL K+DPCR L++
Sbjct: 3568 ISADPSVLESRLAENRSIVESLRDKQNAYDALKQTASELL----ASAPEGDAA-----------------AGDVENKLNRLEKLWKEIEREAVDRGVLLEDVLDKAKHFWSELDSCQKAVDDLR--------------NRLELVEPATGHPEQL-----ADQQEIMAQVASEMER---------------------------ARPRIEALSIAGKQLADYVPDDEKAVIE--NQVANVRGGFSTI-TGLFAEKKRDLIAAMEEAM--------------TFHGD-LQELLKWLDMAEQKLLKMSPVEHAKHMTEIEQLLKELHTFKDEVHERGVAKEQVVATALQLAADAPPHLAATVRQPVADLNTRWSRLNAALAEREHKLENLMLQMGKLASTIAQLTAWMDKTRATLKDIAPP-------------KNAVNLRD-IEIAQCKLVVLSNDIHAHQDSVNAVNRAAQKYIQTSGALDAETSDSLKSMNLKWEDIQKVL-ESLAFDMEVAKKEAENVGGEVEKWQRWLEETESALLSTKPTGGLPETAEFQLDEFKALKLDVEHNASPLEAHLHATEQHLKEEPQDADTWLSKTHGAMKTKWNKVKELLVDREKKLQVAYEQAVALESALNDMEDWIIAAERKLTDQPSISRL-PDVIEKQLAEHESWME---EVAGRKMAMTKH-QASGVHMQYYCEKKDAIPIKNRLVSLKHRVEKISGRTAERAKQLAVTRDEVATWQDGLHDLEHFISDVLVKIAPEPNTTSSLEKLKAKLEEVKEAQRDVTAKQTLFDVTRKRGIGLAERATRSE---YKQISMTNEKMSKKWAEMLKKLRDRLREAEQAVLEGGAFEESMNDLESWVDDELERYQKAEHEPVFADIDGVRALVDEESRRSAERKTKENGVKTVVKKADALMASGVDEKDSIAQAKERLVEKWNQVEEAARHRGNSIKEAEQAAEEFDAKTHALLDWLAVEEQKLKASGL-------DEVEGVKQ------EMDEAKGRYQECLKKGEEILSKCQPAAEPILRNWMRVVEARWKEVSEKVDEREFTLLEQEQKAKEQNEQIEKLAKFAAQKREELNRMIEQPPAQDLDTMEQNICDFANLDSELREQQPEVDAACKSAKKGAR--NPAA----------------------------------------EMLSTEWKKLWLDAMGLQSSLDNQKALLEEMKRLEGWKWEDWKERYVEWNDHAKARVNDLFRRIDRLHTGNVPRQVFIDGIIGSKFPTSRLEMAKVADRFDKGDGM-INAKEFINALRFDA-----SNRNAKPQTDTEKITHEI-ELQKKTCSCCTPYQIEKISENHYRFGDTHIKRMVRILRSTVMVRVGGGWESLDEFLHKHDPCRAKGRLNI 4797
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Celegans
Match: vab-10 (VAB-10B protein [Source:UniProtKB/TrEMBL;Acc:G5EFM4]) HSP 1 Score: 162.155 bits (409), Expect = 3.440e-39 Identity = 276/1400 (19.71%), Postives = 547/1400 (39.07%), Query Frame = 3
Query: 2013 VQSFPEKFNKQFEEVSFLYDQVKKKEIAFHPLKQAINKLLLDDNMSCTQDDIKEQFDGVGIDNEAVLSNDADDLKDHLKEVECLIESVFYKVDNRMSILNELKDLVNLYWSEISEIFSSIKHIQKNAMSIEAFPYNKDDKNKILEPQIAHSDSISTLTDADNKSTTSSVGFELDEKFDEDSKLELPNLTESKFPEEIISQDLVKQQYSSLKKMGKDVTQFVEKLETDLVPKANQIVKIGGQFCELEISEFTESLLEEIGITEDAIEEKDILFNKALYFVDFLSDEIKKVFEWFEVMENRLINFGEITPDQKLIEINLL--ELKSLNTEIFSKAQQLDKIGSAISEILSYPYVPNKNTLEEMKLNIKEKYSELVKGIIEKHNDLQKKYSDIGELDFSLDDNIGILQEFENQIKNLTPQSCKGDPEYTFSCFKNLKFLDDEFNGLQSDFQNLCERISAHGDIEDNTNLRCRGTLSIFGKL--------KEMKILTEQIRVLIPEKYVMIQEILKNEKL-LSKEIHLHIDXXXXXXXXXXXXXXXGFSQESVEQSNLIFSNYIGDLNKQRNSIENSLKLASSHIESCQYDKCTFLSTAIKTLFHKYKRCQHLAAKRKEKLSIYSKLILEYDADKQTLQKDILELE-----NLSIKRMNPSKITVTCAEQIKSIKISIQTADKNFDIYEHLTIRSFDIKCFGNRIDNSQLILELKEYGLKLAKIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIE---NFKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEML-KTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKF--VPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNL--KLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDM----QNVNDIINKHLIFINELSEIDSQIKSLIKDFKLQNPSSFHKAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYRFGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRIANSLDL 6128
+ + P + E + + ++ K+ A+ LKQ ++LL S + D A D+++ L +E L + + + +R +L ++ D +WSE+ ++ ++ ++ +++EP H + + AD + + V E++ + + +L GK + +V E ++ NQ+ + G F + F E + I E+A+ F D ++++ +W ++ E +L+ + + + EI L EL + E+ + +++ + ++ + T+ + ++ ++S L + E+ + L+ +G+L ++ + + +K++ P KN L D Q L I AH D + N + + G L K M + E I+ ++ E E+ K E + E+ +LE+ + L G E+ E F D+ + +E L H++ D T+LS + K+ + + L R++KL + + + ++ ++ I+ E SI R+ P I AE ++ + A + + +H ++ + + D + L ++ KI R ER + E + D +L ++ + V I N K+ L+++K + ++ + F T + G L ++ T + Y + M + + KW ++ + + +++ G ++M + W++D + ++ + D+D V L+DE E + K + K L E+ I E + +++ + R S++E+ Q A+EF++K + L WL E+ LK L D VE +++ ++ E K G+ IL+ C P A + + +RW +++ + ++R L E + IE+L + ++ I D+ QN+ D N + E+D+ KS K + NP++ L+T++ KLW ++ + +L N L E ++L + + WK+ ++ + R+ DLFRR G + F + II S FPT++ + +V + F+K + I ++FI++LR + N N + I H+I E + C+C Y +++ +N YRFG MVR L STVMVR+GGGW+++ EFL K+DPCR L++
Sbjct: 3683 ISADPSVLESRLAENRSIVESLRDKQNAYDALKQTASELL----ASAPEGDAA-----------------AGDVENKLNRLEKLWKEIEREAVDRGVLLEDVLDKAKHFWSELDSCQKAVDDLR--------------NRLELVEPATGHPEQL-----ADQQEIMAQVASEMER---------------------------ARPRIEALSIAGKQLADYVPDDEKAVIE--NQVANVRGGFSTI-TGLFAEKKRDLIAAMEEAM--------------TFHGD-LQELLKWLDMAEQKLLKMSPVEHAKHMTEIEQLLKELHTFKDEVHERGVAKEQVVATALQLAADAPPHLAATVRQPVADLNTRWSRLNAALAEREHKLENLMLQMGKLASTIAQLTAWMDKTRATLKDIAPP-------------KNAVNLRD-IEIAQCKLVVLSNDIHAHQDSVNAVNRAAQKYIQTSGALDAETSDSLKSMNLKWEDIQKVL-ESLAFDMEVAKKEAENVGGEVEKWQRWLEETESALLSTKPTGGLPETAEFQLDEFKALKLDVEHNASPLEAHLHATEQHLKEEPQDADTWLSKTHGAMKTKWNKVKELLVDREKKLQVAYEQAVALESALNDMEDWIIAAERKLTDQPSISRL-PDVIEKQLAEHESWME---EVAGRKMAMTKH-QASGVHMQYYCEKKDAIPIKNRLVSLKHRVEKISGRTAERAKQLAVTRDEVATWQDGLHDLEHFISDVLVKIAPEPNTTSSLEKLKAKLEEVKEAQRDVTAKQTLFDVTRKRGIGLAERATRSE---YKQISMTNEKMSKKWAEMLKKLRDRLREAEQAVLEGGAFEESMNDLESWVDDELERYQKAEHEPVFADIDGVRALVDEESRRSAERKTKENGVKTVVKKADALMASGVDEKDSIAQAKERLVEKWNQVEEAARHRGNSIKEAEQAAEEFDAKTHALLDWLAVEEQKLKASGL-------DEVEGVKQ------EMDEAKGRYQECLKKGEEILSKCQPAAEPILRNWMRVVEARWKEVSEKVDEREFTLLEQEQKAKEQNEQIEKLAKFAAQKREELNRMIEQPPAQDLDTMEQNICDFANLDSELREQQPEVDAACKSAKKGAR--NPAA----------------------------------------EMLSTEWKKLWLDAMGLQSSLDNQKALLEEMKRLEGWKWEDWKERYVEWNDHAKARVNDLFRRIDRLHTGNVPRQVFIDGIIGSKFPTSRLEMAKVADRFDKGDGM-INAKEFINALRFDA-----SNRNAKPQTDTEKITHEI-ELQKKTCSCCTPYQIEKISENHYRFGDTHIKRMVRILRSTVMVRVGGGWESLDEFLHKHDPCRAKGRLNI 4912
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Celegans
Match: vab-10 (VAB-10B protein [Source:UniProtKB/TrEMBL;Acc:G5EFM4]) HSP 1 Score: 162.155 bits (409), Expect = 3.557e-39 Identity = 276/1400 (19.71%), Postives = 547/1400 (39.07%), Query Frame = 3
Query: 2013 VQSFPEKFNKQFEEVSFLYDQVKKKEIAFHPLKQAINKLLLDDNMSCTQDDIKEQFDGVGIDNEAVLSNDADDLKDHLKEVECLIESVFYKVDNRMSILNELKDLVNLYWSEISEIFSSIKHIQKNAMSIEAFPYNKDDKNKILEPQIAHSDSISTLTDADNKSTTSSVGFELDEKFDEDSKLELPNLTESKFPEEIISQDLVKQQYSSLKKMGKDVTQFVEKLETDLVPKANQIVKIGGQFCELEISEFTESLLEEIGITEDAIEEKDILFNKALYFVDFLSDEIKKVFEWFEVMENRLINFGEITPDQKLIEINLL--ELKSLNTEIFSKAQQLDKIGSAISEILSYPYVPNKNTLEEMKLNIKEKYSELVKGIIEKHNDLQKKYSDIGELDFSLDDNIGILQEFENQIKNLTPQSCKGDPEYTFSCFKNLKFLDDEFNGLQSDFQNLCERISAHGDIEDNTNLRCRGTLSIFGKL--------KEMKILTEQIRVLIPEKYVMIQEILKNEKL-LSKEIHLHIDXXXXXXXXXXXXXXXGFSQESVEQSNLIFSNYIGDLNKQRNSIENSLKLASSHIESCQYDKCTFLSTAIKTLFHKYKRCQHLAAKRKEKLSIYSKLILEYDADKQTLQKDILELE-----NLSIKRMNPSKITVTCAEQIKSIKISIQTADKNFDIYEHLTIRSFDIKCFGNRIDNSQLILELKEYGLKLAKIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIE---NFKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEML-KTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKF--VPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNL--KLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDM----QNVNDIINKHLIFINELSEIDSQIKSLIKDFKLQNPSSFHKAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYRFGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRIANSLDL 6128
+ + P + E + + ++ K+ A+ LKQ ++LL S + D A D+++ L +E L + + + +R +L ++ D +WSE+ ++ ++ ++ +++EP H + + AD + + V E++ + + +L GK + +V E ++ NQ+ + G F + F E + I E+A+ F D ++++ +W ++ E +L+ + + + EI L EL + E+ + +++ + ++ + T+ + ++ ++S L + E+ + L+ +G+L ++ + + +K++ P KN L D Q L I AH D + N + + G L K M + E I+ ++ E E+ K E + E+ +LE+ + L G E+ E F D+ + +E L H++ D T+LS + K+ + + L R++KL + + + ++ ++ I+ E SI R+ P I AE ++ + A + + +H ++ + + D + L ++ KI R ER + E + D +L ++ + V I N K+ L+++K + ++ + F T + G L ++ T + Y + M + + KW ++ + + +++ G ++M + W++D + ++ + D+D V L+DE E + K + K L E+ I E + +++ + R S++E+ Q A+EF++K + L WL E+ LK L D VE +++ ++ E K G+ IL+ C P A + + +RW +++ + ++R L E + IE+L + ++ I D+ QN+ D N + E+D+ KS K + NP++ L+T++ KLW ++ + +L N L E ++L + + WK+ ++ + R+ DLFRR G + F + II S FPT++ + +V + F+K + I ++FI++LR + N N + I H+I E + C+C Y +++ +N YRFG MVR L STVMVR+GGGW+++ EFL K+DPCR L++
Sbjct: 3443 ISADPSVLESRLAENRSIVESLRDKQNAYDALKQTASELL----ASAPEGDAA-----------------AGDVENKLNRLEKLWKEIEREAVDRGVLLEDVLDKAKHFWSELDSCQKAVDDLR--------------NRLELVEPATGHPEQL-----ADQQEIMAQVASEMER---------------------------ARPRIEALSIAGKQLADYVPDDEKAVIE--NQVANVRGGFSTI-TGLFAEKKRDLIAAMEEAM--------------TFHGD-LQELLKWLDMAEQKLLKMSPVEHAKHMTEIEQLLKELHTFKDEVHERGVAKEQVVATALQLAADAPPHLAATVRQPVADLNTRWSRLNAALAEREHKLENLMLQMGKLASTIAQLTAWMDKTRATLKDIAPP-------------KNAVNLRD-IEIAQCKLVVLSNDIHAHQDSVNAVNRAAQKYIQTSGALDAETSDSLKSMNLKWEDIQKVL-ESLAFDMEVAKKEAENVGGEVEKWQRWLEETESALLSTKPTGGLPETAEFQLDEFKALKLDVEHNASPLEAHLHATEQHLKEEPQDADTWLSKTHGAMKTKWNKVKELLVDREKKLQVAYEQAVALESALNDMEDWIIAAERKLTDQPSISRL-PDVIEKQLAEHESWME---EVAGRKMAMTKH-QASGVHMQYYCEKKDAIPIKNRLVSLKHRVEKISGRTAERAKQLAVTRDEVATWQDGLHDLEHFISDVLVKIAPEPNTTSSLEKLKAKLEEVKEAQRDVTAKQTLFDVTRKRGIGLAERATRSE---YKQISMTNEKMSKKWAEMLKKLRDRLREAEQAVLEGGAFEESMNDLESWVDDELERYQKAEHEPVFADIDGVRALVDEESRRSAERKTKENGVKTVVKKADALMASGVDEKDSIAQAKERLVEKWNQVEEAARHRGNSIKEAEQAAEEFDAKTHALLDWLAVEEQKLKASGL-------DEVEGVKQ------EMDEAKGRYQECLKKGEEILSKCQPAAEPILRNWMRVVEARWKEVSEKVDEREFTLLEQEQKAKEQNEQIEKLAKFAAQKREELNRMIEQPPAQDLDTMEQNICDFANLDSELREQQPEVDAACKSAKKGAR--NPAA----------------------------------------EMLSTEWKKLWLDAMGLQSSLDNQKALLEEMKRLEGWKWEDWKERYVEWNDHAKARVNDLFRRIDRLHTGNVPRQVFIDGIIGSKFPTSRLEMAKVADRFDKGDGM-INAKEFINALRFDA-----SNRNAKPQTDTEKITHEI-ELQKKTCSCCTPYQIEKISENHYRFGDTHIKRMVRILRSTVMVRVGGGWESLDEFLHKHDPCRAKGRLNI 4672
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Celegans
Match: vab-10 (VAB-10B protein [Source:UniProtKB/TrEMBL;Acc:G5EFM4]) HSP 1 Score: 161.77 bits (408), Expect = 3.952e-39 Identity = 276/1400 (19.71%), Postives = 547/1400 (39.07%), Query Frame = 3
Query: 2013 VQSFPEKFNKQFEEVSFLYDQVKKKEIAFHPLKQAINKLLLDDNMSCTQDDIKEQFDGVGIDNEAVLSNDADDLKDHLKEVECLIESVFYKVDNRMSILNELKDLVNLYWSEISEIFSSIKHIQKNAMSIEAFPYNKDDKNKILEPQIAHSDSISTLTDADNKSTTSSVGFELDEKFDEDSKLELPNLTESKFPEEIISQDLVKQQYSSLKKMGKDVTQFVEKLETDLVPKANQIVKIGGQFCELEISEFTESLLEEIGITEDAIEEKDILFNKALYFVDFLSDEIKKVFEWFEVMENRLINFGEITPDQKLIEINLL--ELKSLNTEIFSKAQQLDKIGSAISEILSYPYVPNKNTLEEMKLNIKEKYSELVKGIIEKHNDLQKKYSDIGELDFSLDDNIGILQEFENQIKNLTPQSCKGDPEYTFSCFKNLKFLDDEFNGLQSDFQNLCERISAHGDIEDNTNLRCRGTLSIFGKL--------KEMKILTEQIRVLIPEKYVMIQEILKNEKL-LSKEIHLHIDXXXXXXXXXXXXXXXGFSQESVEQSNLIFSNYIGDLNKQRNSIENSLKLASSHIESCQYDKCTFLSTAIKTLFHKYKRCQHLAAKRKEKLSIYSKLILEYDADKQTLQKDILELE-----NLSIKRMNPSKITVTCAEQIKSIKISIQTADKNFDIYEHLTIRSFDIKCFGNRIDNSQLILELKEYGLKLAKIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIE---NFKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEML-KTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKF--VPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNL--KLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDM----QNVNDIINKHLIFINELSEIDSQIKSLIKDFKLQNPSSFHKAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYRFGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRIANSLDL 6128
+ + P + E + + ++ K+ A+ LKQ ++LL S + D A D+++ L +E L + + + +R +L ++ D +WSE+ ++ ++ ++ +++EP H + + AD + + V E++ + + +L GK + +V E ++ NQ+ + G F + F E + I E+A+ F D ++++ +W ++ E +L+ + + + EI L EL + E+ + +++ + ++ + T+ + ++ ++S L + E+ + L+ +G+L ++ + + +K++ P KN L D Q L I AH D + N + + G L K M + E I+ ++ E E+ K E + E+ +LE+ + L G E+ E F D+ + +E L H++ D T+LS + K+ + + L R++KL + + + ++ ++ I+ E SI R+ P I AE ++ + A + + +H ++ + + D + L ++ KI R ER + E + D +L ++ + V I N K+ L+++K + ++ + F T + G L ++ T + Y + M + + KW ++ + + +++ G ++M + W++D + ++ + D+D V L+DE E + K + K L E+ I E + +++ + R S++E+ Q A+EF++K + L WL E+ LK L D VE +++ ++ E K G+ IL+ C P A + + +RW +++ + ++R L E + IE+L + ++ I D+ QN+ D N + E+D+ KS K + NP++ L+T++ KLW ++ + +L N L E ++L + + WK+ ++ + R+ DLFRR G + F + II S FPT++ + +V + F+K + I ++FI++LR + N N + I H+I E + C+C Y +++ +N YRFG MVR L STVMVR+GGGW+++ EFL K+DPCR L++
Sbjct: 3570 ISADPSVLESRLAENRSIVESLRDKQNAYDALKQTASELL----ASAPEGDAA-----------------AGDVENKLNRLEKLWKEIEREAVDRGVLLEDVLDKAKHFWSELDSCQKAVDDLR--------------NRLELVEPATGHPEQL-----ADQQEIMAQVASEMER---------------------------ARPRIEALSIAGKQLADYVPDDEKAVIE--NQVANVRGGFSTI-TGLFAEKKRDLIAAMEEAM--------------TFHGD-LQELLKWLDMAEQKLLKMSPVEHAKHMTEIEQLLKELHTFKDEVHERGVAKEQVVATALQLAADAPPHLAATVRQPVADLNTRWSRLNAALAEREHKLENLMLQMGKLASTIAQLTAWMDKTRATLKDIAPP-------------KNAVNLRD-IEIAQCKLVVLSNDIHAHQDSVNAVNRAAQKYIQTSGALDAETSDSLKSMNLKWEDIQKVL-ESLAFDMEVAKKEAENVGGEVEKWQRWLEETESALLSTKPTGGLPETAEFQLDEFKALKLDVEHNASPLEAHLHATEQHLKEEPQDADTWLSKTHGAMKTKWNKVKELLVDREKKLQVAYEQAVALESALNDMEDWIIAAERKLTDQPSISRL-PDVIEKQLAEHESWME---EVAGRKMAMTKH-QASGVHMQYYCEKKDAIPIKNRLVSLKHRVEKISGRTAERAKQLAVTRDEVATWQDGLHDLEHFISDVLVKIAPEPNTTSSLEKLKAKLEEVKEAQRDVTAKQTLFDVTRKRGIGLAERATRSE---YKQISMTNEKMSKKWAEMLKKLRDRLREAEQAVLEGGAFEESMNDLESWVDDELERYQKAEHEPVFADIDGVRALVDEESRRSAERKTKENGVKTVVKKADALMASGVDEKDSIAQAKERLVEKWNQVEEAARHRGNSIKEAEQAAEEFDAKTHALLDWLAVEEQKLKASGL-------DEVEGVKQ------EMDEAKGRYQECLKKGEEILSKCQPAAEPILRNWMRVVEARWKEVSEKVDEREFTLLEQEQKAKEQNEQIEKLAKFAAQKREELNRMIEQPPAQDLDTMEQNICDFANLDSELREQQPEVDAACKSAKKGAR--NPAA----------------------------------------EMLSTEWKKLWLDAMGLQSSLDNQKALLEEMKRLEGWKWEDWKERYVEWNDHAKARVNDLFRRIDRLHTGNVPRQVFIDGIIGSKFPTSRLEMAKVADRFDKGDGM-INAKEFINALRFDA-----SNRNAKPQTDTEKITHEI-ELQKKTCSCCTPYQIEKISENHYRFGDTHIKRMVRILRSTVMVRVGGGWESLDEFLHKHDPCRAKGRLNI 4799
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Fly
Match: shot (gene:FBgn0013733 transcript:FBtr0273222) HSP 1 Score: 251.136 bits (640), Expect = 4.862e-66 Identity = 258/1130 (22.83%), Postives = 500/1130 (44.25%), Query Frame = 3
Query: 2856 LSDEIKKVFEWFEVMEN----------RLINFGEITPDQKLIEINLLELKSLNTEIFSKAQQLDKIGSAISEILSYPYVPNKNTLEEMKLNIKEKYSELVKGIIEKHNDLQKKYSDIGELDFSLDDNIGILQEFENQIKNLTPQSCKGDPEYTFSCFKNLKFLDDEFNGLQSDFQNLCERISAHGDIEDNTNLRCRGTLSIFGKLKEMKILTEQIRVLIPEKYVMIQEILKNEKLLS---KEIHLHIDXXX--------XXXXXXXXXXXXGFSQESVEQSNLIFSNYIGDLNKQRNSIENSLKLASSHIESCQYDKCTF--LSTAIKTLFHKYKRCQHLAAKRKEKLSIYSKLILEYDADKQTLQKDILELENLSIKRMNPSKITVTCAEQIKSIKISIQTADKNFDIYEHLTIRSFDIKCFGNRIDNSQLILELKEYGLKLAKIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIENF------KDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLKLRMLNEIKNYEES----DIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAE-KLLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFKL----QNPSSFHKAPTK-------RYHDRVEFYKNIWRNSKDSLNKSTDDLS-TNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYRFGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCR 6107
L D ++K E+ E ++N + + G + D ++ + +LKS E+ +++ + E+ ++ E + ++ L++G++E+ L+ +G+ +L++ + + + ++ + L P GDP+ LK L ++ I AH + D N R + E E++R L E ++Q+ + L +E H +I ++ P G + + EQ Y +L++ R +E +I+ K + L ++TL ++ A+ +K KL I K E+ Q + + + E L S++ T Q++ K+ + + + L + +K F + D + L + ++ + ER R + Y+E + F+D+++ + +L E+ +++ +P K+ ++ KLK +QL + + + T+R G L ++ D + +L +ML L+ +W ++ + + L L+ +G DA+ + W+ + ++ + + GDL+TV+ L + E ++ + + L ++ E S ++ ++E+++ + + V +R + LQ +L A++ N+++ L WL AE KL K+ P +E E+++ H +K ++ + T + I+ +P A + + RW ++ + +R KL + L SL+ IEEL+ + + N ++P ++ V +I H F+ + +++ K +L + PS K P + DR+ Y + S + S +L + R L T++ +W LS E + L +HL+ L + E+ R F ++ W+K FL Y+ K RLTDLFR+ G + F + I+ + F T+ ++ V ++F++N I +Q+FI +LRP+ Q K N +DK IHD +++ V +CTC ++ V + G+ KYRFG + KL +VR L STVMVR+GGGW + EFL+KNDPCR
Sbjct: 3998 LIDAMEKAMEFHETLQNLLKFLTKAEDKFAHLGAVGSDIDAVKRQIEQLKSFKDEVDPHMVEVEALNRQAVELTERTSPEQAASIREPLSVVNRRWEALLRGMVERQKQLEHALLHLGQFQHALNELLVWINKTDSTLDQLKP--IPGDPQLLEVELAKLKVLAND--------------IQAHQNSVDTLNDAGRQLIETEKGSVEASTTQEKLRKLNNEWKQLLQKASDRQHELEEALREAHGYIAEVQDILGWLGDVDAVIGASKPVGGLPETATEQLERFMEVY-NELDENRPKVETIQAQGQEYIKRQNQMKVSSSNLQHTLRTLKQRWDAVVSRASDKKIKLEIALKEATEFHDTLQAFVEWLTQAEKLLSNAEPVSRVLETIQAQMEEHKVLQKDVSTHREAMLLLDKKGTHLKYFSQKQDVILIKNLLVSVQHRWERVVSKAAERTRALDHGYKEAREFNDAWSGMMQYLQETEQVLDQIIEEATASKEPQKIKKYIGKLKETHRQLGAKQSVYDGTMRTGKNLLERAPKGD--RPVLDKMLIELKEQWTRVWSKSIDRQRKLEEALLLSGQFSDALGELLDWLKKAKSRLNENGPVHGDLETVQGLCEHHKHIEQDLQKRAAQMQGV----LKTGRDLERSGNNPEVGRQLDEMQSIWEEVKSAVAKRGERLQVALVDAEKLNARVQALFDWLDHAEHKLRYAKNAP--DDEKVSREMMDIHMDFMKDLRVREREKTETFEYAEDIINKAYPDAIPIIKNWLSIIQQRWEEVRQWAINRESKLEQHLQSLKDLDDTIEELLAWLSGLEGTLLNLKHEQLPDEIPPVEKLIEDHKEFMENTARRQNEVDRACKPRQLPPGARKPSRSGKTPVRITPTRDTPDRDRLPHY-----GPRFSPSTSGPELEFRSPRAKLLWTKWRDVWMLSWERQRLLNDHLLYLKDVERARNFSWDDWRKRFLKYMNHKKSRLTDLFRKMDKDNNGMIPRDVFIDGILNTKFDTSGLEMKAVADLFDRNGEGLIDWQEFIAALRPD--WQERKPANDSDK------IHDEVKRLVMLCTCRQKFRVFQVGEGKYRFGDSQKLRLVRILRSTVMVRVGGGWVALDEFLQKNDPCR 5089 HSP 2 Score: 51.9878 bits (123), Expect = 8.969e-6 Identity = 40/201 (19.90%), Postives = 92/201 (45.77%), Query Frame = 3
Query: 4416 LKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEI---NNHKEDFKNLKLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSL-PKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNI 5006
L+ L +WN ++ + L+ L+++G +A+ ++ W++D+ D V+ + E + + N NL + N + E + I + ++ +F + +QR L+E++++AK F+ K++ L++WL E+ +K L P +E + + I +H+ L ++ K ++ ++
Sbjct: 3111 LEKLNDRWNDLKERMNERDRRLDVALLQSGKFQEALAGLSKWLSDTEEMVANQKPPSSDYKVVKAQLQEQKFLKKMLLDRQNSMGSLANLGKEVANHCEPGERASIEKQLNDLMKRFDALTDGAEQRELDLEEAMEVAKRFHDKISPLELWLDNTERSVKAMELIPT--DEEKIQQRIREHDRLHDEILGKKPDFSDLADV 3309
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Fly
Match: shot (gene:FBgn0013733 transcript:FBtr0304853) HSP 1 Score: 251.136 bits (640), Expect = 4.918e-66 Identity = 260/1136 (22.89%), Postives = 502/1136 (44.19%), Query Frame = 3
Query: 2856 LSDEIKKVFEWFEVMEN----------RLINFGEITPDQKLIEINLLELKSLNTEIFSKAQQLDKIGSAISEILSYPYVPNKNTLEEMKLNIKEKYSELVKGIIEKHNDLQKKYSDIGELDFSLDDNIGILQEFENQIKNLTPQSCKGDPEYTFSCFKNLKFLDDEFNGLQSDFQNLCERISAHGDIEDNTNLRCRGTLSIFGKLKEMKILTEQIRVLIPEKYVMIQEILKNEKLLS---KEIHLHIDXXX--------XXXXXXXXXXXXGFSQESVEQSNLIFSNYIGDLNKQRNSIENSLKLASSHIESCQYDKCTF--LSTAIKTLFHKYKRCQHLAAKRKEKLSIYSKLILEYDADKQTLQKDILELENLSIKRMNPSKITVTCAEQIKSIKISIQTADKNFDIYEHLTIRSFDIKCFGNRIDNSQLILELKEYGLKLAKIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIENF------KDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLKLRMLNEIKNYEES----DIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAE-KLLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFKL----QNPSSFHKAPTK-RYHDRVE------FYKNIWRNSKD-SLNKSTDDLST------NTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYRFGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCR 6107
L D ++K E+ E ++N + + G + D ++ + +LKS E+ +++ + E+ ++ E + ++ L++G++E+ L+ +G+ +L++ + + + ++ + L P GDP+ LK L ++ I AH + D N R + E E++R L E ++Q+ + L +E H +I ++ P G + + EQ Y +L++ R +E +I+ K + L ++TL ++ A+ +K KL I K E+ Q + + + E L S++ T Q++ K+ + + + L + +K F + D + L + ++ + ER R + Y+E + F+D+++ + +L E+ +++ +P K+ ++ KLK +QL + + + T+R G L ++ D + +L +ML L+ +W ++ + + L L+ +G DA+ + W+ + ++ + + GDL+TV+ L + E ++ + + L ++ E S ++ ++E+++ + + V +R + LQ +L A++ N+++ L WL AE KL K+ P +E E+++ H +K ++ + T + I+ +P A + + RW ++ + +R KL + L SL+ IEEL+ + + N ++P ++ V +I H F+ + +++ K +L + PS K P + HD E + ++ S+D LN S + R L T++ +W LS E + L +HL+ L + E+ R F ++ W+K FL Y+ K RLTDLFR+ G + F + I+ + F T+ ++ V ++F++N I +Q+FI +LRP+ Q K N +DK IHD +++ V +CTC ++ V + G+ KYRFG + KL +VR L STVMVR+GGGW + EFL+KNDPCR
Sbjct: 4068 LIDAMEKAMEFHETLQNLLKFLTKAEDKFAHLGAVGSDIDAVKRQIEQLKSFKDEVDPHMVEVEALNRQAVELTERTSPEQAASIREPLSVVNRRWEALLRGMVERQKQLEHALLHLGQFQHALNELLVWINKTDSTLDQLKP--IPGDPQLLEVELAKLKVLAND--------------IQAHQNSVDTLNDAGRQLIETEKGSVEASTTQEKLRKLNNEWKQLLQKASDRQHELEEALREAHGYIAEVQDILGWLGDVDAVIGASKPVGGLPETATEQLERFMEVY-NELDENRPKVETIQAQGQEYIKRQNQMKVSSSNLQHTLRTLKQRWDAVVSRASDKKIKLEIALKEATEFHDTLQAFVEWLTQAEKLLSNAEPVSRVLETIQAQMEEHKVLQKDVSTHREAMLLLDKKGTHLKYFSQKQDVILIKNLLVSVQHRWERVVSKAAERTRALDHGYKEAREFNDAWSGMMQYLQETEQVLDQIIEEATASKEPQKIKKYIGKLKETHRQLGAKQSVYDGTMRTGKNLLERAPKGD--RPVLDKMLIELKEQWTRVWSKSIDRQRKLEEALLLSGQFSDALGELLDWLKKAKSRLNENGPVHGDLETVQGLCEHHKHIEQDLQKRAAQMQGV----LKTGRDLERSGNNPEVGRQLDEMQSIWEEVKSAVAKRGERLQVALVDAEKLNARVQALFDWLDHAEHKLRYAKNAP--DDEKVSREMMDIHMDFMKDLRVREREKTETFEYAEDIINKAYPDAIPIIKNWLSIIQQRWEEVRQWAINRESKLEQHLQSLKDLDDTIEELLAWLSGLEGTLLNLKHEQLPDEIPPVEKLIEDHKEFMENTARRQNEVDRACKPRQLPPGARKPSRSGKTPVRGSSHDLREQSPDGTLRRQSFKGSRDQGLNARKGSTSGPELEFRSPRAKLLWTKWRDVWMLSWERQRLLNDHLLYLKDVERARNFSWDDWRKRFLKYMNHKKSRLTDLFRKMDKDNNGMIPRDVFIDGILNTKFDTSGLEMKAVADLFDRNGEGLIDWQEFIAALRPD--WQERKPANDSDK------IHDEVKRLVMLCTCRQKFRVFQVGEGKYRFGDSQKLRLVRILRSTVMVRVGGGWVALDEFLQKNDPCR 5170 HSP 2 Score: 52.373 bits (124), Expect = 8.320e-6 Identity = 40/201 (19.90%), Postives = 92/201 (45.77%), Query Frame = 3
Query: 4416 LKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEI---NNHKEDFKNLKLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSL-PKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNI 5006
L+ L +WN ++ + L+ L+++G +A+ ++ W++D+ D V+ + E + + N NL + N + E + I + ++ +F + +QR L+E++++AK F+ K++ L++WL E+ +K L P +E + + I +H+ L ++ K ++ ++
Sbjct: 3181 LEKLNDRWNDLKERMNERDRRLDVALLQSGKFQEALAGLSKWLSDTEEMVANQKPPSSDYKVVKAQLQEQKFLKKMLLDRQNSMGSLANLGKEVANHCEPGERASIEKQLNDLMKRFDALTDGAEQRELDLEEAMEVAKRFHDKISPLELWLDNTERSVKAMELIPT--DEEKIQQRIREHDRLHDEILGKKPDFSDLADV 3379
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Fly
Match: shot (gene:FBgn0013733 transcript:FBtr0304847) HSP 1 Score: 251.136 bits (640), Expect = 4.918e-66 Identity = 260/1136 (22.89%), Postives = 502/1136 (44.19%), Query Frame = 3
Query: 2856 LSDEIKKVFEWFEVMEN----------RLINFGEITPDQKLIEINLLELKSLNTEIFSKAQQLDKIGSAISEILSYPYVPNKNTLEEMKLNIKEKYSELVKGIIEKHNDLQKKYSDIGELDFSLDDNIGILQEFENQIKNLTPQSCKGDPEYTFSCFKNLKFLDDEFNGLQSDFQNLCERISAHGDIEDNTNLRCRGTLSIFGKLKEMKILTEQIRVLIPEKYVMIQEILKNEKLLS---KEIHLHIDXXX--------XXXXXXXXXXXXGFSQESVEQSNLIFSNYIGDLNKQRNSIENSLKLASSHIESCQYDKCTF--LSTAIKTLFHKYKRCQHLAAKRKEKLSIYSKLILEYDADKQTLQKDILELENLSIKRMNPSKITVTCAEQIKSIKISIQTADKNFDIYEHLTIRSFDIKCFGNRIDNSQLILELKEYGLKLAKIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIENF------KDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLKLRMLNEIKNYEES----DIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAE-KLLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFKL----QNPSSFHKAPTK-RYHDRVE------FYKNIWRNSKD-SLNKSTDDLST------NTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYRFGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCR 6107
L D ++K E+ E ++N + + G + D ++ + +LKS E+ +++ + E+ ++ E + ++ L++G++E+ L+ +G+ +L++ + + + ++ + L P GDP+ LK L ++ I AH + D N R + E E++R L E ++Q+ + L +E H +I ++ P G + + EQ Y +L++ R +E +I+ K + L ++TL ++ A+ +K KL I K E+ Q + + + E L S++ T Q++ K+ + + + L + +K F + D + L + ++ + ER R + Y+E + F+D+++ + +L E+ +++ +P K+ ++ KLK +QL + + + T+R G L ++ D + +L +ML L+ +W ++ + + L L+ +G DA+ + W+ + ++ + + GDL+TV+ L + E ++ + + L ++ E S ++ ++E+++ + + V +R + LQ +L A++ N+++ L WL AE KL K+ P +E E+++ H +K ++ + T + I+ +P A + + RW ++ + +R KL + L SL+ IEEL+ + + N ++P ++ V +I H F+ + +++ K +L + PS K P + HD E + ++ S+D LN S + R L T++ +W LS E + L +HL+ L + E+ R F ++ W+K FL Y+ K RLTDLFR+ G + F + I+ + F T+ ++ V ++F++N I +Q+FI +LRP+ Q K N +DK IHD +++ V +CTC ++ V + G+ KYRFG + KL +VR L STVMVR+GGGW + EFL+KNDPCR
Sbjct: 4068 LIDAMEKAMEFHETLQNLLKFLTKAEDKFAHLGAVGSDIDAVKRQIEQLKSFKDEVDPHMVEVEALNRQAVELTERTSPEQAASIREPLSVVNRRWEALLRGMVERQKQLEHALLHLGQFQHALNELLVWINKTDSTLDQLKP--IPGDPQLLEVELAKLKVLAND--------------IQAHQNSVDTLNDAGRQLIETEKGSVEASTTQEKLRKLNNEWKQLLQKASDRQHELEEALREAHGYIAEVQDILGWLGDVDAVIGASKPVGGLPETATEQLERFMEVY-NELDENRPKVETIQAQGQEYIKRQNQMKVSSSNLQHTLRTLKQRWDAVVSRASDKKIKLEIALKEATEFHDTLQAFVEWLTQAEKLLSNAEPVSRVLETIQAQMEEHKVLQKDVSTHREAMLLLDKKGTHLKYFSQKQDVILIKNLLVSVQHRWERVVSKAAERTRALDHGYKEAREFNDAWSGMMQYLQETEQVLDQIIEEATASKEPQKIKKYIGKLKETHRQLGAKQSVYDGTMRTGKNLLERAPKGD--RPVLDKMLIELKEQWTRVWSKSIDRQRKLEEALLLSGQFSDALGELLDWLKKAKSRLNENGPVHGDLETVQGLCEHHKHIEQDLQKRAAQMQGV----LKTGRDLERSGNNPEVGRQLDEMQSIWEEVKSAVAKRGERLQVALVDAEKLNARVQALFDWLDHAEHKLRYAKNAP--DDEKVSREMMDIHMDFMKDLRVREREKTETFEYAEDIINKAYPDAIPIIKNWLSIIQQRWEEVRQWAINRESKLEQHLQSLKDLDDTIEELLAWLSGLEGTLLNLKHEQLPDEIPPVEKLIEDHKEFMENTARRQNEVDRACKPRQLPPGARKPSRSGKTPVRGSSHDLREQSPDGTLRRQSFKGSRDQGLNARKGSTSGPELEFRSPRAKLLWTKWRDVWMLSWERQRLLNDHLLYLKDVERARNFSWDDWRKRFLKYMNHKKSRLTDLFRKMDKDNNGMIPRDVFIDGILNTKFDTSGLEMKAVADLFDRNGEGLIDWQEFIAALRPD--WQERKPANDSDK------IHDEVKRLVMLCTCRQKFRVFQVGEGKYRFGDSQKLRLVRILRSTVMVRVGGGWVALDEFLQKNDPCR 5170 HSP 2 Score: 52.373 bits (124), Expect = 8.320e-6 Identity = 40/201 (19.90%), Postives = 92/201 (45.77%), Query Frame = 3
Query: 4416 LKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEI---NNHKEDFKNLKLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSL-PKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNI 5006
L+ L +WN ++ + L+ L+++G +A+ ++ W++D+ D V+ + E + + N NL + N + E + I + ++ +F + +QR L+E++++AK F+ K++ L++WL E+ +K L P +E + + I +H+ L ++ K ++ ++
Sbjct: 3181 LEKLNDRWNDLKERMNERDRRLDVALLQSGKFQEALAGLSKWLSDTEEMVANQKPPSSDYKVVKAQLQEQKFLKKMLLDRQNSMGSLANLGKEVANHCEPGERASIEKQLNDLMKRFDALTDGAEQRELDLEEAMEVAKRFHDKISPLELWLDNTERSVKAMELIPT--DEEKIQQRIREHDRLHDEILGKKPDFSDLADV 3379
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Fly
Match: shot (gene:FBgn0013733 transcript:FBtr0273224) HSP 1 Score: 250.751 bits (639), Expect = 5.468e-66 Identity = 258/1130 (22.83%), Postives = 500/1130 (44.25%), Query Frame = 3
Query: 2856 LSDEIKKVFEWFEVMEN----------RLINFGEITPDQKLIEINLLELKSLNTEIFSKAQQLDKIGSAISEILSYPYVPNKNTLEEMKLNIKEKYSELVKGIIEKHNDLQKKYSDIGELDFSLDDNIGILQEFENQIKNLTPQSCKGDPEYTFSCFKNLKFLDDEFNGLQSDFQNLCERISAHGDIEDNTNLRCRGTLSIFGKLKEMKILTEQIRVLIPEKYVMIQEILKNEKLLS---KEIHLHIDXXX--------XXXXXXXXXXXXGFSQESVEQSNLIFSNYIGDLNKQRNSIENSLKLASSHIESCQYDKCTF--LSTAIKTLFHKYKRCQHLAAKRKEKLSIYSKLILEYDADKQTLQKDILELENLSIKRMNPSKITVTCAEQIKSIKISIQTADKNFDIYEHLTIRSFDIKCFGNRIDNSQLILELKEYGLKLAKIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIENF------KDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLKLRMLNEIKNYEES----DIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAE-KLLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFKL----QNPSSFHKAPTK-------RYHDRVEFYKNIWRNSKDSLNKSTDDLS-TNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYRFGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCR 6107
L D ++K E+ E ++N + + G + D ++ + +LKS E+ +++ + E+ ++ E + ++ L++G++E+ L+ +G+ +L++ + + + ++ + L P GDP+ LK L ++ I AH + D N R + E E++R L E ++Q+ + L +E H +I ++ P G + + EQ Y +L++ R +E +I+ K + L ++TL ++ A+ +K KL I K E+ Q + + + E L S++ T Q++ K+ + + + L + +K F + D + L + ++ + ER R + Y+E + F+D+++ + +L E+ +++ +P K+ ++ KLK +QL + + + T+R G L ++ D + +L +ML L+ +W ++ + + L L+ +G DA+ + W+ + ++ + + GDL+TV+ L + E ++ + + L ++ E S ++ ++E+++ + + V +R + LQ +L A++ N+++ L WL AE KL K+ P +E E+++ H +K ++ + T + I+ +P A + + RW ++ + +R KL + L SL+ IEEL+ + + N ++P ++ V +I H F+ + +++ K +L + PS K P + DR+ Y + S + S +L + R L T++ +W LS E + L +HL+ L + E+ R F ++ W+K FL Y+ K RLTDLFR+ G + F + I+ + F T+ ++ V ++F++N I +Q+FI +LRP+ Q K N +DK IHD +++ V +CTC ++ V + G+ KYRFG + KL +VR L STVMVR+GGGW + EFL+KNDPCR
Sbjct: 3998 LIDAMEKAMEFHETLQNLLKFLTKAEDKFAHLGAVGSDIDAVKRQIEQLKSFKDEVDPHMVEVEALNRQAVELTERTSPEQAASIREPLSVVNRRWEALLRGMVERQKQLEHALLHLGQFQHALNELLVWINKTDSTLDQLKP--IPGDPQLLEVELAKLKVLAND--------------IQAHQNSVDTLNDAGRQLIETEKGSVEASTTQEKLRKLNNEWKQLLQKASDRQHELEEALREAHGYIAEVQDILGWLGDVDAVIGASKPVGGLPETATEQLERFMEVY-NELDENRPKVETIQAQGQEYIKRQNQMKVSSSNLQHTLRTLKQRWDAVVSRASDKKIKLEIALKEATEFHDTLQAFVEWLTQAEKLLSNAEPVSRVLETIQAQMEEHKVLQKDVSTHREAMLLLDKKGTHLKYFSQKQDVILIKNLLVSVQHRWERVVSKAAERTRALDHGYKEAREFNDAWSGMMQYLQETEQVLDQIIEEATASKEPQKIKKYIGKLKETHRQLGAKQSVYDGTMRTGKNLLERAPKGD--RPVLDKMLIELKEQWTRVWSKSIDRQRKLEEALLLSGQFSDALGELLDWLKKAKSRLNENGPVHGDLETVQGLCEHHKHIEQDLQKRAAQMQGV----LKTGRDLERSGNNPEVGRQLDEMQSIWEEVKSAVAKRGERLQVALVDAEKLNARVQALFDWLDHAEHKLRYAKNAP--DDEKVSREMMDIHMDFMKDLRVREREKTETFEYAEDIINKAYPDAIPIIKNWLSIIQQRWEEVRQWAINRESKLEQHLQSLKDLDDTIEELLAWLSGLEGTLLNLKHEQLPDEIPPVEKLIEDHKEFMENTARRQNEVDRACKPRQLPPGARKPSRSGKTPVRITPTRDTPDRDRLPHY-----GPRFSPSTSGPELEFRSPRAKLLWTKWRDVWMLSWERQRLLNDHLLYLKDVERARNFSWDDWRKRFLKYMNHKKSRLTDLFRKMDKDNNGMIPRDVFIDGILNTKFDTSGLEMKAVADLFDRNGEGLIDWQEFIAALRPD--WQERKPANDSDK------IHDEVKRLVMLCTCRQKFRVFQVGEGKYRFGDSQKLRLVRILRSTVMVRVGGGWVALDEFLQKNDPCR 5089 HSP 2 Score: 51.9878 bits (123), Expect = 8.673e-6 Identity = 40/201 (19.90%), Postives = 92/201 (45.77%), Query Frame = 3
Query: 4416 LKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEI---NNHKEDFKNLKLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSL-PKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNI 5006
L+ L +WN ++ + L+ L+++G +A+ ++ W++D+ D V+ + E + + N NL + N + E + I + ++ +F + +QR L+E++++AK F+ K++ L++WL E+ +K L P +E + + I +H+ L ++ K ++ ++
Sbjct: 3111 LEKLNDRWNDLKERMNERDRRLDVALLQSGKFQEALAGLSKWLSDTEEMVANQKPPSSDYKVVKAQLQEQKFLKKMLLDRQNSMGSLANLGKEVANHCEPGERASIEKQLNDLMKRFDALTDGAEQRELDLEEAMEVAKRFHDKISPLELWLDNTERSVKAMELIPT--DEEKIQQRIREHDRLHDEILGKKPDFSDLADV 3309
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Fly
Match: shot (gene:FBgn0013733 transcript:FBtr0273225) HSP 1 Score: 249.98 bits (637), Expect = 1.144e-65 Identity = 258/1130 (22.83%), Postives = 500/1130 (44.25%), Query Frame = 3
Query: 2856 LSDEIKKVFEWFEVMEN----------RLINFGEITPDQKLIEINLLELKSLNTEIFSKAQQLDKIGSAISEILSYPYVPNKNTLEEMKLNIKEKYSELVKGIIEKHNDLQKKYSDIGELDFSLDDNIGILQEFENQIKNLTPQSCKGDPEYTFSCFKNLKFLDDEFNGLQSDFQNLCERISAHGDIEDNTNLRCRGTLSIFGKLKEMKILTEQIRVLIPEKYVMIQEILKNEKLLS---KEIHLHIDXXX--------XXXXXXXXXXXXGFSQESVEQSNLIFSNYIGDLNKQRNSIENSLKLASSHIESCQYDKCTF--LSTAIKTLFHKYKRCQHLAAKRKEKLSIYSKLILEYDADKQTLQKDILELENLSIKRMNPSKITVTCAEQIKSIKISIQTADKNFDIYEHLTIRSFDIKCFGNRIDNSQLILELKEYGLKLAKIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIENF------KDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLKLRMLNEIKNYEES----DIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAE-KLLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFKL----QNPSSFHKAPTK-------RYHDRVEFYKNIWRNSKDSLNKSTDDLS-TNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYRFGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCR 6107
L D ++K E+ E ++N + + G + D ++ + +LKS E+ +++ + E+ ++ E + ++ L++G++E+ L+ +G+ +L++ + + + ++ + L P GDP+ LK L ++ I AH + D N R + E E++R L E ++Q+ + L +E H +I ++ P G + + EQ Y +L++ R +E +I+ K + L ++TL ++ A+ +K KL I K E+ Q + + + E L S++ T Q++ K+ + + + L + +K F + D + L + ++ + ER R + Y+E + F+D+++ + +L E+ +++ +P K+ ++ KLK +QL + + + T+R G L ++ D + +L +ML L+ +W ++ + + L L+ +G DA+ + W+ + ++ + + GDL+TV+ L + E ++ + + L ++ E S ++ ++E+++ + + V +R + LQ +L A++ N+++ L WL AE KL K+ P +E E+++ H +K ++ + T + I+ +P A + + RW ++ + +R KL + L SL+ IEEL+ + + N ++P ++ V +I H F+ + +++ K +L + PS K P + DR+ Y + S + S +L + R L T++ +W LS E + L +HL+ L + E+ R F ++ W+K FL Y+ K RLTDLFR+ G + F + I+ + F T+ ++ V ++F++N I +Q+FI +LRP+ Q K N +DK IHD +++ V +CTC ++ V + G+ KYRFG + KL +VR L STVMVR+GGGW + EFL+KNDPCR
Sbjct: 4109 LIDAMEKAMEFHETLQNLLKFLTKAEDKFAHLGAVGSDIDAVKRQIEQLKSFKDEVDPHMVEVEALNRQAVELTERTSPEQAASIREPLSVVNRRWEALLRGMVERQKQLEHALLHLGQFQHALNELLVWINKTDSTLDQLKP--IPGDPQLLEVELAKLKVLAND--------------IQAHQNSVDTLNDAGRQLIETEKGSVEASTTQEKLRKLNNEWKQLLQKASDRQHELEEALREAHGYIAEVQDILGWLGDVDAVIGASKPVGGLPETATEQLERFMEVY-NELDENRPKVETIQAQGQEYIKRQNQMKVSSSNLQHTLRTLKQRWDAVVSRASDKKIKLEIALKEATEFHDTLQAFVEWLTQAEKLLSNAEPVSRVLETIQAQMEEHKVLQKDVSTHREAMLLLDKKGTHLKYFSQKQDVILIKNLLVSVQHRWERVVSKAAERTRALDHGYKEAREFNDAWSGMMQYLQETEQVLDQIIEEATASKEPQKIKKYIGKLKETHRQLGAKQSVYDGTMRTGKNLLERAPKGD--RPVLDKMLIELKEQWTRVWSKSIDRQRKLEEALLLSGQFSDALGELLDWLKKAKSRLNENGPVHGDLETVQGLCEHHKHIEQDLQKRAAQMQGV----LKTGRDLERSGNNPEVGRQLDEMQSIWEEVKSAVAKRGERLQVALVDAEKLNARVQALFDWLDHAEHKLRYAKNAP--DDEKVSREMMDIHMDFMKDLRVREREKTETFEYAEDIINKAYPDAIPIIKNWLSIIQQRWEEVRQWAINRESKLEQHLQSLKDLDDTIEELLAWLSGLEGTLLNLKHEQLPDEIPPVEKLIEDHKEFMENTARRQNEVDRACKPRQLPPGARKPSRSGKTPVRITPTRDTPDRDRLPHY-----GPRFSPSTSGPELEFRSPRAKLLWTKWRDVWMLSWERQRLLNDHLLYLKDVERARNFSWDDWRKRFLKYMNHKKSRLTDLFRKMDKDNNGMIPRDVFIDGILNTKFDTSGLEMKAVADLFDRNGEGLIDWQEFIAALRPD--WQERKPANDSDK------IHDEVKRLVMLCTCRQKFRVFQVGEGKYRFGDSQKLRLVRILRSTVMVRVGGGWVALDEFLQKNDPCR 5200 HSP 2 Score: 51.9878 bits (123), Expect = 9.051e-6 Identity = 40/201 (19.90%), Postives = 92/201 (45.77%), Query Frame = 3
Query: 4416 LKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEI---NNHKEDFKNLKLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSL-PKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNI 5006
L+ L +WN ++ + L+ L+++G +A+ ++ W++D+ D V+ + E + + N NL + N + E + I + ++ +F + +QR L+E++++AK F+ K++ L++WL E+ +K L P +E + + I +H+ L ++ K ++ ++
Sbjct: 3222 LEKLNDRWNDLKERMNERDRRLDVALLQSGKFQEALAGLSKWLSDTEEMVANQKPPSSDYKVVKAQLQEQKFLKKMLLDRQNSMGSLANLGKEVANHCEPGERASIEKQLNDLMKRFDALTDGAEQRELDLEEAMEVAKRFHDKISPLELWLDNTERSVKAMELIPT--DEEKIQQRIREHDRLHDEILGKKPDFSDLADV 3420
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Zebrafish
Match: DST (dystonin [Source:HGNC Symbol;Acc:HGNC:1090]) HSP 1 Score: 247.669 bits (631), Expect = 6.725e-65 Identity = 186/727 (25.58%), Postives = 350/727 (48.14%), Query Frame = 3
Query: 4119 KIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIEN---FKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLK--LRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKI-KSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELS----EIDSQIKSLIKDFKLQNPSSFH-------KAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDLST-NTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYR---------------------------------------------FGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRI 6110
K+ +R ER R+ + A + K F +S++ L WL+ES ++ + P K+ L + K K L +++ + T R G LKDK +++D + L ML LR KW+ + V ++ L L+ +G DA++ + W+ + D + GD+D V LID + + E+ + LK R L E + + S ++ ++E+ ++ + + L+++L A+EF+S ++ L WL +AE+ L+ +LP +E L LIE+H+ +K+++E + ++ ++G+ IL+ CHP + + +R+ ++ + + ++L+ +L L+ + L+E+L+ + + + + +P +++ V +I +H F+ E++ ++D K+ + P H + P KR + + + S + T N R+N L +++ ++W L+++ + L + L L E ++ FDF+ W+K ++ ++ K R+ D FRR + G +EF + I+ S FPT++ + V +IF+++ + I Y +F+ +L P + + K + DK I D + ++VA C C R+ V++ G NKYR FG + +L +VR L STVMVR+GGGW + EFL KNDPCR+
Sbjct: 2896 KVVQRSVERGRLLDDARKRAKQFHESWSKLMEWLEESERALDTELEIANDPDKIKTQLTQHKEFQKALGSKHSVYDTTSRTGRALKDKTSLQD-DNQKLDHMLSELRDKWDTVCGKSVERQNKLEEALLFSGQFTDALQALIDWLYKVEPQLAEDQPVHGDIDLVLNLIDSHKVFQKELGKRTGSVQALKRSARDLIESSHDDSSWVKVQMQELSTRWETVCSLSVSKQTRLEQALSQAEEFHSTVHILLEWLAEAEQSLRFHGALPD--DEEALRALIEQHKEFMKRLEEKRLALSKATSMGEAILSICHPDSITTIKHWNTIIKARFEEVTAWARQHQQRLSSALDELQATQELLEKLLNWLQWAETTLGEKDKDPMPQELEEVKALIAEHQTFLEEMTRKQPDVDKVTKTHKRKATADPPVQSHIPVLDKGRTPRKRSPTQTMY----------TAAASQAQIETKNPRVNLLVSKWQQVWLLALDRRRKLNDALDRLEELKEFANFDFDVWRKRYMRWMNHKKSRVMDFFRRIDKDQDGKVTRQEFIDGILSSKFPTSRLEMSAVADIFDRDGDGYIDYYEFVAALHPNKDAY--KPLTDADK------IEDEVTRQVAKCKCPKRFQVEQIGANKYRFYLGNQFGLVHCLHDPLIADETITNRDNQNLTPHLHVAVLESCILFGDSQQLRLVRILRSTVMVRVGGGWMALDEFLVKNDPCRV 3601 HSP 2 Score: 53.5286 bits (127), Expect = 4.177e-6 Identity = 79/418 (18.90%), Postives = 175/418 (41.87%), Query Frame = 3
Query: 4122 IQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIENFKDQPTKVLDHLDKLKNLCKQL------SCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHK---EDFKNLKLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEK-LLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKT-GSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCD--MQNVNDIINKHLIFINELSEIDSQIKSLIK 5336
+ K +ER+ I +A + F DS N+ +D ++E D + V L+ +K ++L + Q L + + L K +++ ++ + E + L+ W + I+ + L L+ G A+ + W+ + K GD +E+ + + H+ +N++ HK E ++ E ++N +E + ++ + +QR + L +L A+ F+ ++ ++ WL+ +E+ LL K++ + A E + H L ++ + + G+ IL P + L + +W + ++ ++R KL E+L +++ I L + + + + P ++ + I++H +F+ E++ QI L K
Sbjct: 2460 LNKTWKERVEILEEAMQAAVQFQDSLQNMFDSVD----IMEGKLDSMSPVGTDLETVKQQIEELKEFKGDAYQLQIEMERLNHQAGLLLKKVMDEDDRTSISEPMNELKMLWENLDKKIINRQHKLEGALLALGQFQHALDELLTWLTHTEELLGEQRKACGDPKAIEIELAKHHVLQNDVLAHKSTVEAVNRAGCDLMESSAGEEARGLQNKLENLNQRWKNILEKTEQRRQLLDSALLQAQGFHGEIEDMQRWLKDSERQLLASKAVGGLPDTAR--EQLNTHLELCSTLEVKEEIYKHLLERGQQILAMT-PEGQDSTTELDLRNLQDKWESVQAKVKERKVKLEEALAMATDFHNSLQDFI----NWLTQAEQTLTMVSPASLILETIMFQIDEHKVFVTEVNSHRDQIMELDK 2866
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Zebrafish
Match: macf1a (microtubule actin crosslinking factor 1a [Source:ZFIN;Acc:ZDB-GENE-030131-3606]) HSP 1 Score: 234.187 bits (596), Expect = 9.166e-61 Identity = 177/679 (26.07%), Postives = 331/679 (48.75%), Query Frame = 3
Query: 4119 KIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIEN---FKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTV-EDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLK--LRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFK---LQNPSSFHKAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYR------FGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRI 6110
K+ +R +R R ++A + K F +++ L WL+E+ +++ ++P K+ L K K K L + + T+R G ++DK T+ D +K L +L +R KW+ + V + L L+ +G +A++ + W+ + D + GDLD V L+D + + E+ + LK R L E + + ++ ++E+ N++ + LQ++L+ A+EF + + L WL +AE+ L+ + + + E L L+ H ++ V+E + + +G+ IL CHP + I +R+ ++ + ++ ++L +L L + AL+EEL+ + + + D+ + +I +H +F+ E++ + + K +K ++PSS R + + S N RL L +++ ++W L+++ + L + L L E ++ FDF+ W+K ++ ++ K R+ D FRR + G +EF + I+ S FPT+K + V +IF+++ + I Y +F+ +L P + + + DK I D + ++VA C C R+ V++ G+NKYR FG + +L +VR L STVMVR+GGGW + EFL KNDPCR+
Sbjct: 4508 KLVQRSLDRGRHLDEARKRAKQFHEAWRKLTDWLEEAESRLDSELEISNEPDKIKIQLAKHKEFQKSLGSKQPVYDTTVRSGKAMRDKATLPADTQK--LDNLLGEVRDKWDTVCGKSVERQHKLEEALLFSGQFAEALQALVDWLYRVEPQLAEDQPVHGDLDLVSNLMDCHKVFQKELGKRTSSVQALKRSARELMETGRDDTAWVKVQLQELSNRWDTVCALSVSKQTRLQQALKQAEEFRTAVQMLLEWLSEAEQTLRFRGVLPEEAET-LQALLHTHRDFMQTVEEKRVDVNKAAGMGEAILAVCHPDCITTIKHWITIIRARFEEVLTWAKQHEQRLETALTELLNNAALLEELLSWLQWAETTLVQRDTEPLTQDILQLKTLITEHQVFMEEMTRKQPDVDKVTKTYKRKPAEHPSSLSDRKGARK-----------HQQQQQQHHSVQVTGGNPRLTQLCSRWQQVWLLALDRQRKLNDALDRLEELKEFANFDFDVWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILASKFPTSKLEMTAVADIFDRDCDGYIDYYEFVAALHPNKDAY--RPTTDADK------IEDEVTRQVAQCKCAKRFQVEQIGENKYRFFLGNQFGDSQQLRLVRILRSTVMVRVGGGWMALDEFLVKNDPCRV 5164 HSP 2 Score: 71.633 bits (174), Expect = 1.673e-11 Identity = 83/418 (19.86%), Postives = 177/418 (42.34%), Query Frame = 3
Query: 4122 IQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIENFKDQPT---KVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHK---EDFKNLKLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVIL-----TNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCD--MQNVNDIINKHLIFINELSEIDSQIKSLIK 5336
+ + RER+ ++A + D+ + +LD + + + + T V +++LK ++ Q + G L K + + ++ ++ E L LR W+ + I + L L+ G A+ + W++ + A + D +E+ + + H+ N++ +H+ E + +L E + +R+ ++E+ + + +R K L+ +LQ A+ F+ +L E WLR+ E L + P E +++H L Q+ + + G+ IL + P T L ++W +N++ EDR KL E++ G + +++ I L + + + + P + V I++H +F+NE++ Q+ +L K
Sbjct: 4068 LNRTWRERMERLDEAMTASVQYQDALQGMFDYLDNAVIKLCDMPAVGTDLSTVKQQIEELKQYKVEVYQQQIDMEKLSHQGELLLRKVS-DQTDRDMIQEPLTELRHLWDNLVDKITVRQHKLEGALLALGQFQHALSELQSWLSHTHATLDTQRPISSDPKAIEIELAKHHVLRNDVLSHRSTVETVNSAGSELLESSPGDEINHLRDQLDELNRSWKNLLLKTDERQKLLEAALQQAEGFHGELEEFLQWLRRTESQLS-AAKPTGGLPETAREQLQQHMELQAQLAQRGEHYHRLLDHGESILLARGGEDAGPGTTQTQQNLAM-LQNKWASLNTKMEDRRGKLEEAVSLATGFQSSLQDTI----NWLTQAEQTLNMAQPPSLILDTVLFQIDEHKVFVNEVNTHREQVLALEK 4478 HSP 3 Score: 62.7734 bits (151), Expect = 6.895e-9 Identity = 85/412 (20.63%), Postives = 170/412 (41.26%), Query Frame = 3
Query: 4140 ERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIENFKDQ---PTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVED--PEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNL-----KLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSLPKFQNEADLVEL-IEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCH-PMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESL---GSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSL 5330
ER ++ A ++ + F WL + + K P + L + L QL+ + ++H L +G + ED P + L L+ KW + + + L + +++ W+ + LDTV IDE + NE+N H+E L +LR + ++ I+N + ++ ++ V R + L E+ + AK+F+ +L WL +AE ++ S + NE D +++ + KH+ K + + GK + P T ++ L+ + +W+ + +S +R KL E+L G + + + +Y+V L + Q + D+ V+++++ H +F EL + S +++L
Sbjct: 4293 ERQKLLEAALQQAEGFHGELEEFLQWLRRTESQLSAAKPTGGLPETAREQLQQHMELQAQLAQRGEHYHRLLDHGESILLARGGEDAGPGTTQTQQNLAMLQNKWASLNTKMEDRRGKLEEAVSLATGFQSSLQDTINWLTQAEQTLNMAQPPSLILDTVLFQIDEHKVFVNEVNTHREQVLALEKAGSQLRFASLKQDV--VLIKNLLLSVQARWDKLVQRSLDRGRHLDEARKRAKQFHEAWRKLTDWLEEAES--RLDSELEISNEPDKIKIQLAKHKEFQKSLGSKQPVYDTTVRSGKAMRDKATLPADTQKLDNLLGEVRDKWDTVCGKSVERQHKLEEALLFSGQFAEALQALVDWLYRVEPQLAEDQ-----PVHGDLDLVSNLMDCHKVFQKELGKRTSSVQAL 4695
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Zebrafish
Match: macf1a (microtubule actin crosslinking factor 1a [Source:ZFIN;Acc:ZDB-GENE-030131-3606]) HSP 1 Score: 233.032 bits (593), Expect = 1.794e-60 Identity = 178/686 (25.95%), Postives = 333/686 (48.54%), Query Frame = 3
Query: 4119 KIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIEN---FKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTV-EDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLK--LRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFK---LQNPSSFHKAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYR------FGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRIANSLDLQ 6131
K+ +R +R R ++A + K F +++ L WL+E+ +++ ++P K+ L K K K L + + T+R G ++DK T+ D +K L +L +R KW+ + V + L L+ +G +A++ + W+ + D + GDLD V L+D + + E+ + LK R L E + + ++ ++E+ N++ + LQ++L+ A+EF + + L WL +AE+ L+ + + + E L L+ H ++ V+E + + +G+ IL CHP + I +R+ ++ + ++ ++L +L L + AL+EEL+ + + + D+ + +I +H +F+ E++ + + K +K ++PSS R + + S N RL L +++ ++W L+++ + L + L L E ++ FDF+ W+K ++ ++ K R+ D FRR + G +EF + I+ S FPT+K + V +IF+++ + I Y +F+ +L P + + + DK I D + ++VA C C R+ V++ G+NKYR FG + +L +VR L STVMVR+GGGW + EFL KNDPCR +L+
Sbjct: 4876 KLVQRSLDRGRHLDEARKRAKQFHEAWRKLTDWLEEAESRLDSELEISNEPDKIKIQLAKHKEFQKSLGSKQPVYDTTVRSGKAMRDKATLPADTQK--LDNLLGEVRDKWDTVCGKSVERQHKLEEALLFSGQFAEALQALVDWLYRVEPQLAEDQPVHGDLDLVSNLMDCHKVFQKELGKRTSSVQALKRSARELMETGRDDTAWVKVQLQELSNRWDTVCALSVSKQTRLQQALKQAEEFRTAVQMLLEWLSEAEQTLRFRGVLPEEAET-LQALLHTHRDFMQTVEEKRVDVNKAAGMGEAILAVCHPDCITTIKHWITIIRARFEEVLTWAKQHEQRLETALTELLNNAALLEELLSWLQWAETTLVQRDTEPLTQDILQLKTLITEHQVFMEEMTRKQPDVDKVTKTYKRKPAEHPSSLSDRKGARK-----------HQQQQQQHHSVQVTGGNPRLTQLCSRWQQVWLLALDRQRKLNDALDRLEELKEFANFDFDVWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILASKFPTSKLEMTAVADIFDRDCDGYIDYYEFVAALHPNKDAY--RPTTDADK------IEDEVTRQVAQCKCAKRFQVEQIGENKYRFFLGNQFGDSQQLRLVRILRSTVMVRVGGGWMALDEFLVKNDPCRARGRTNLE 5539 HSP 2 Score: 71.2478 bits (173), Expect = 2.106e-11 Identity = 83/418 (19.86%), Postives = 177/418 (42.34%), Query Frame = 3
Query: 4122 IQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIENFKDQPT---KVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHK---EDFKNLKLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVIL-----TNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCD--MQNVNDIINKHLIFINELSEIDSQIKSLIK 5336
+ + RER+ ++A + D+ + +LD + + + + T V +++LK ++ Q + G L K + + ++ ++ E L LR W+ + I + L L+ G A+ + W++ + A + D +E+ + + H+ N++ +H+ E + +L E + +R+ ++E+ + + +R K L+ +LQ A+ F+ +L E WLR+ E L + P E +++H L Q+ + + G+ IL + P T L ++W +N++ EDR KL E++ G + +++ I L + + + + P + V I++H +F+NE++ Q+ +L K
Sbjct: 4436 LNRTWRERMERLDEAMTASVQYQDALQGMFDYLDNAVIKLCDMPAVGTDLSTVKQQIEELKQYKVEVYQQQIDMEKLSHQGELLLRKVS-DQTDRDMIQEPLTELRHLWDNLVDKITVRQHKLEGALLALGQFQHALSELQSWLSHTHATLDTQRPISSDPKAIEIELAKHHVLRNDVLSHRSTVETVNSAGSELLESSPGDEINHLRDQLDELNRSWKNLLLKTDERQKLLEAALQQAEGFHGELEEFLQWLRRTESQLS-AAKPTGGLPETAREQLQQHMELQAQLAQRGEHYHRLLDHGESILLARGGEDAGPGTTQTQQNLAM-LQNKWASLNTKMEDRRGKLEEAVSLATGFQSSLQDTI----NWLTQAEQTLNMAQPPSLILDTVLFQIDEHKVFVNEVNTHREQVLALEK 4846 HSP 3 Score: 62.3882 bits (150), Expect = 8.895e-9 Identity = 85/412 (20.63%), Postives = 170/412 (41.26%), Query Frame = 3
Query: 4140 ERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIENFKDQ---PTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVED--PEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNL-----KLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSLPKFQNEADLVEL-IEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCH-PMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESL---GSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSL 5330
ER ++ A ++ + F WL + + K P + L + L QL+ + ++H L +G + ED P + L L+ KW + + + L + +++ W+ + LDTV IDE + NE+N H+E L +LR + ++ I+N + ++ ++ V R + L E+ + AK+F+ +L WL +AE ++ S + NE D +++ + KH+ K + + GK + P T ++ L+ + +W+ + +S +R KL E+L G + + + +Y+V L + Q + D+ V+++++ H +F EL + S +++L
Sbjct: 4661 ERQKLLEAALQQAEGFHGELEEFLQWLRRTESQLSAAKPTGGLPETAREQLQQHMELQAQLAQRGEHYHRLLDHGESILLARGGEDAGPGTTQTQQNLAMLQNKWASLNTKMEDRRGKLEEAVSLATGFQSSLQDTINWLTQAEQTLNMAQPPSLILDTVLFQIDEHKVFVNEVNTHREQVLALEKAGSQLRFASLKQDV--VLIKNLLLSVQARWDKLVQRSLDRGRHLDEARKRAKQFHEAWRKLTDWLEEAES--RLDSELEISNEPDKIKIQLAKHKEFQKSLGSKQPVYDTTVRSGKAMRDKATLPADTQKLDNLLGEVRDKWDTVCGKSVERQHKLEEALLFSGQFAEALQALVDWLYRVEPQLAEDQ-----PVHGDLDLVSNLMDCHKVFQKELGKRTSSVQAL 5063
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Zebrafish
Match: macf1b (microtubule actin crosslinking factor 1b [Source:ZFIN;Acc:ZDB-GENE-100922-36]) HSP 1 Score: 120.553 bits (301), Expect = 1.120e-29 Identity = 66/185 (35.68%), Postives = 107/185 (57.84%), Query Frame = 3
Query: 5577 EQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYRFGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRIANSLDLQ 6131
E+ FDFN W+K ++ +I R+ D+FR + G +EF + ++ S FPTN + V NIF+ N + I Y +F+ +L P R + ++++ I++ + ++V+ C C R+ V++ N+YRFG + +L MVR L ST+MVR+GGGW + EFL KNDPCR+ +L+
Sbjct: 3 EEFANFDFNIWRKRYMQWISHLKSRILDVFRSIDRDQDGRITHKEFIDSVLASKFPTNVLEMTAVANIFDVNGDGFIDYYEFVSALHPSR-DPYRRTVDIDQ-------INEEVSRQVSQCNCPKRFQVEQISANRYRFGDSQQLRMVRILRSTLMVRVGGGWTALDEFLVKNDPCRVKGRTNLK 179
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Zebrafish
Match: gas2l1 (growth arrest-specific 2 like 1 [Source:ZFIN;Acc:ZDB-GENE-070705-38]) HSP 1 Score: 67.3958 bits (163), Expect = 4.297e-11 Identity = 25/70 (35.71%), Postives = 45/70 (64.29%), Query Frame = 3
Query: 5904 IHDIIEKEVAVCTCTLRYYVKREGDNKYRFGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRIA 6113
+ +++ + + C+C ++ + + + KY+ G +S L +R L + VMVR+GGGW T+ +L K+DPCR A
Sbjct: 229 LDELVREILGQCSCPSQFPMTKVSEGKYKVGDSSALIFIRVLRTHVMVRVGGGWDTLEHYLDKHDPCRCA 298
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Xenopus
Match: rab11fip2 (RAB11 family interacting protein 2 (class I) [Source:Xenbase;Acc:XB-GENE-5947669]) HSP 1 Score: 265.774 bits (678), Expect = 3.100e-70 Identity = 189/682 (27.71%), Postives = 345/682 (50.59%), Query Frame = 3
Query: 4122 IQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIEN---FKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLK--LRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKS-LPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFK--LQNPSSFHKAPTKRYHDRVEFYKNIW-RNSKDSLNKSTDDLS---TNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYR-------FGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRI 6110
+ +R ER R+ + A + K F +++ L WL+ES +++ + P K+ L K K K L ++ + T R G LK+K T+ D + L +ML +R KW+ + + ++ L L+ +G DA++ + W+ + D + GD+D V LID + + E+ + LK R L E + S ++ ++E+ ++ N + L+E+L A+ F++ ++ L WL AE+ L+ LP +E L LIE+H+ +++V+E ++ + ++G+ IL CHP + + I +R+ ++ + ++ ++L +L L L+E L+ + + + + +IP D+ V +I +H IF+ E+++ + + K +K +++ + + P ++ + W R+ SL S S N R+N L +++ ++W L++E + L + L L E + FDF+ W+K ++ ++ K R+ D FRR + G +EF I+ S FPT++ + V +IF+++ + I Y +F+ +L P + + K I DK I D + ++VA C C R+ V++ G+NKYR FG + +L +VR L STVMVR+GGGW + EFL KNDPCR+
Sbjct: 5285 VVQRSLERGRMLDDARKRSKQFHEAFDKLMEWLEESERSLDSELEIANDPDKIKTQLTKHKEFQKTLGGKHTVYDTTTRSGRSLKEKATLTD-DIQKLDDMLSEIRDKWDTVCGKSIERQNKLEEALLISGQFTDALQALIDWLYRIEPQLAEDQPVHGDIDIVMNLIDNHKVFQKELGKRSSSIQALKRSARELIEGSRDDSSWVKAQMQELGTRWETVCNLSVSKQTRLEEALHQAESFHAAVHALLEWLADAEQALRFHGILP--DDEDSLRLLIEQHKEFMRRVEEKRSELNKGASMGEAILAICHPDSITTIKHWITIIRARFEEVLTWTKQHQQRLAVALDDLIAKKELLESLLAWLQWSEMTLNEKDKEDIPQDLDEVKALIEEHQIFMEEMTQKQPDVDKVTKTYKRKVESTTGQSQIPV------LDKGRGGWKRSPTPSLYPSGTQTSLETKNPRVNLLVSKWQQVWLLALERRRKLNDALDRLEELREFANFDFDVWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIEGILSSKFPTSRLEMSAVADIFDRDGDGYIDYYEFVAALHPNKDAY--KPITDADK------IEDEVTRQVAKCKCAKRFQVEQIGENKYRVISLLFKFGDSQQLRLVRILRSTVMVRVGGGWMALDEFLVKNDPCRV 5949 HSP 2 Score: 66.6254 bits (161), Expect = 7.796e-10 Identity = 83/429 (19.35%), Postives = 177/429 (41.26%), Query Frame = 3
Query: 4122 IQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIENFKDQPTKVL---DHLDKLKNL-----CKQLSCQYANFH--CTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLKLR---MLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLL----KIKSLPKFQNEA-----DLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCD--MQNVNDIINKHLIFINELSEIDSQIKSLIK 5336
+ K +ER+ ++A + + D L W+D + + +E+ T ++ + +LK +Q+ + N L+ + DK VEDP L L+ WN + I+ + L L+ G A+ + W+ + + GD +E+ + + H+ +N++ HK + +K ++ E S + + +E + ++ + ++ ++R K L +L A+ F+S++ ++ WL E+ L + LP+ E +L L E E + K++ + M + C +++ + +W ++++ +R KL E+L G +++ I L + + + I P + V I++H +F+ E++ QI L K
Sbjct: 4848 LNKTWKERVEKLDEAMQAAVQYQDGLQALYDWVDIATLKVESMPPIGTDLVTVKQQIVELKQFKTDTYQQQIELERLNHQKELLLKKVTDENDKQAVEDP--------LSELKLLWNSLEDKIINRQHKLEGALLALGPFQHALDELTTWMTHTEGLLNEQKSVGGDPKAIEIELAKHHVLQNDVLAHKSTVETVKKAGNDLIESSAGEEASSLNSRLESLNQRWQIILDKTEKRMKLLDSALFQAQGFHSEIEDMHQWLTDIERQLLASKPVGGLPETAREQLSAHLELCSLFEVKEEIYKRLMQKSQQM----------MERCTDDPEANIDQDVINLKEKWESVDTKLSERKAKLEEALEVATGFHNSLQDFI----NWLTQAEQTLTIASPPSLILDTVLFQIDEHKVFVTEVNSHRDQIMELDK 5254
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Xenopus
Match: rab11fip2 (RAB11 family interacting protein 2 (class I) [Source:Xenbase;Acc:XB-GENE-5947669]) HSP 1 Score: 265.388 bits (677), Expect = 4.799e-70 Identity = 189/682 (27.71%), Postives = 345/682 (50.59%), Query Frame = 3
Query: 4122 IQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIEN---FKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLK--LRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKS-LPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFK--LQNPSSFHKAPTKRYHDRVEFYKNIW-RNSKDSLNKSTDDLS---TNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYR-------FGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRI 6110
+ +R ER R+ + A + K F +++ L WL+ES +++ + P K+ L K K K L ++ + T R G LK+K T+ D + L +ML +R KW+ + + ++ L L+ +G DA++ + W+ + D + GD+D V LID + + E+ + LK R L E + S ++ ++E+ ++ N + L+E+L A+ F++ ++ L WL AE+ L+ LP +E L LIE+H+ +++V+E ++ + ++G+ IL CHP + + I +R+ ++ + ++ ++L +L L L+E L+ + + + + +IP D+ V +I +H IF+ E+++ + + K +K +++ + + P ++ + W R+ SL S S N R+N L +++ ++W L++E + L + L L E + FDF+ W+K ++ ++ K R+ D FRR + G +EF I+ S FPT++ + V +IF+++ + I Y +F+ +L P + + K I DK I D + ++VA C C R+ V++ G+NKYR FG + +L +VR L STVMVR+GGGW + EFL KNDPCR+
Sbjct: 5486 VVQRSLERGRMLDDARKRSKQFHEAFDKLMEWLEESERSLDSELEIANDPDKIKTQLTKHKEFQKTLGGKHTVYDTTTRSGRSLKEKATLTD-DIQKLDDMLSEIRDKWDTVCGKSIERQNKLEEALLISGQFTDALQALIDWLYRIEPQLAEDQPVHGDIDIVMNLIDNHKVFQKELGKRSSSIQALKRSARELIEGSRDDSSWVKAQMQELGTRWETVCNLSVSKQTRLEEALHQAESFHAAVHALLEWLADAEQALRFHGILP--DDEDSLRLLIEQHKEFMRRVEEKRSELNKGASMGEAILAICHPDSITTIKHWITIIRARFEEVLTWTKQHQQRLAVALDDLIAKKELLESLLAWLQWSEMTLNEKDKEDIPQDLDEVKALIEEHQIFMEEMTQKQPDVDKVTKTYKRKVESTTGQSQIPV------LDKGRGGWKRSPTPSLYPSGTQTSLETKNPRVNLLVSKWQQVWLLALERRRKLNDALDRLEELREFANFDFDVWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIEGILSSKFPTSRLEMSAVADIFDRDGDGYIDYYEFVAALHPNKDAY--KPITDADK------IEDEVTRQVAKCKCAKRFQVEQIGENKYRVISLLFKFGDSQQLRLVRILRSTVMVRVGGGWMALDEFLVKNDPCRV 6150 HSP 2 Score: 66.2402 bits (160), Expect = 8.633e-10 Identity = 83/429 (19.35%), Postives = 177/429 (41.26%), Query Frame = 3
Query: 4122 IQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIENFKDQPTKVL---DHLDKLKNL-----CKQLSCQYANFH--CTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLKLR---MLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLL----KIKSLPKFQNEA-----DLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCD--MQNVNDIINKHLIFINELSEIDSQIKSLIK 5336
+ K +ER+ ++A + + D L W+D + + +E+ T ++ + +LK +Q+ + N L+ + DK VEDP L L+ WN + I+ + L L+ G A+ + W+ + + GD +E+ + + H+ +N++ HK + +K ++ E S + + +E + ++ + ++ ++R K L +L A+ F+S++ ++ WL E+ L + LP+ E +L L E E + K++ + M + C +++ + +W ++++ +R KL E+L G +++ I L + + + I P + V I++H +F+ E++ QI L K
Sbjct: 5049 LNKTWKERVEKLDEAMQAAVQYQDGLQALYDWVDIATLKVESMPPIGTDLVTVKQQIVELKQFKTDTYQQQIELERLNHQKELLLKKVTDENDKQAVEDP--------LSELKLLWNSLEDKIINRQHKLEGALLALGPFQHALDELTTWMTHTEGLLNEQKSVGGDPKAIEIELAKHHVLQNDVLAHKSTVETVKKAGNDLIESSAGEEASSLNSRLESLNQRWQIILDKTEKRMKLLDSALFQAQGFHSEIEDMHQWLTDIERQLLASKPVGGLPETAREQLSAHLELCSLFEVKEEIYKRLMQKSQQM----------MERCTDDPEANIDQDVINLKEKWESVDTKLSERKAKLEEALEVATGFHNSLQDFI----NWLTQAEQTLTIASPPSLILDTVLFQIDEHKVFVTEVNSHRDQIMELDK 5455
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Xenopus
Match: rab11fip2 (RAB11 family interacting protein 2 (class I) [Source:Xenbase;Acc:XB-GENE-5947669]) HSP 1 Score: 265.003 bits (676), Expect = 6.063e-70 Identity = 189/682 (27.71%), Postives = 345/682 (50.59%), Query Frame = 3
Query: 4122 IQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIEN---FKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLK--LRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKS-LPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFK--LQNPSSFHKAPTKRYHDRVEFYKNIW-RNSKDSLNKSTDDLS---TNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYR-------FGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRI 6110
+ +R ER R+ + A + K F +++ L WL+ES +++ + P K+ L K K K L ++ + T R G LK+K T+ D + L +ML +R KW+ + + ++ L L+ +G DA++ + W+ + D + GD+D V LID + + E+ + LK R L E + S ++ ++E+ ++ N + L+E+L A+ F++ ++ L WL AE+ L+ LP +E L LIE+H+ +++V+E ++ + ++G+ IL CHP + + I +R+ ++ + ++ ++L +L L L+E L+ + + + + +IP D+ V +I +H IF+ E+++ + + K +K +++ + + P ++ + W R+ SL S S N R+N L +++ ++W L++E + L + L L E + FDF+ W+K ++ ++ K R+ D FRR + G +EF I+ S FPT++ + V +IF+++ + I Y +F+ +L P + + K I DK I D + ++VA C C R+ V++ G+NKYR FG + +L +VR L STVMVR+GGGW + EFL KNDPCR+
Sbjct: 5438 VVQRSLERGRMLDDARKRSKQFHEAFDKLMEWLEESERSLDSELEIANDPDKIKTQLTKHKEFQKTLGGKHTVYDTTTRSGRSLKEKATLTD-DIQKLDDMLSEIRDKWDTVCGKSIERQNKLEEALLISGQFTDALQALIDWLYRIEPQLAEDQPVHGDIDIVMNLIDNHKVFQKELGKRSSSIQALKRSARELIEGSRDDSSWVKAQMQELGTRWETVCNLSVSKQTRLEEALHQAESFHAAVHALLEWLADAEQALRFHGILP--DDEDSLRLLIEQHKEFMRRVEEKRSELNKGASMGEAILAICHPDSITTIKHWITIIRARFEEVLTWTKQHQQRLAVALDDLIAKKELLESLLAWLQWSEMTLNEKDKEDIPQDLDEVKALIEEHQIFMEEMTQKQPDVDKVTKTYKRKVESTTGQSQIPV------LDKGRGGWKRSPTPSLYPSGTQTSLETKNPRVNLLVSKWQQVWLLALERRRKLNDALDRLEELREFANFDFDVWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIEGILSSKFPTSRLEMSAVADIFDRDGDGYIDYYEFVAALHPNKDAY--KPITDADK------IEDEVTRQVAKCKCAKRFQVEQIGENKYRVISLLFKFGDSQQLRLVRILRSTVMVRVGGGWMALDEFLVKNDPCRV 6102 HSP 2 Score: 66.2402 bits (160), Expect = 9.787e-10 Identity = 83/429 (19.35%), Postives = 177/429 (41.26%), Query Frame = 3
Query: 4122 IQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIENFKDQPTKVL---DHLDKLKNL-----CKQLSCQYANFH--CTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLKLR---MLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLL----KIKSLPKFQNEA-----DLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCD--MQNVNDIINKHLIFINELSEIDSQIKSLIK 5336
+ K +ER+ ++A + + D L W+D + + +E+ T ++ + +LK +Q+ + N L+ + DK VEDP L L+ WN + I+ + L L+ G A+ + W+ + + GD +E+ + + H+ +N++ HK + +K ++ E S + + +E + ++ + ++ ++R K L +L A+ F+S++ ++ WL E+ L + LP+ E +L L E E + K++ + M + C +++ + +W ++++ +R KL E+L G +++ I L + + + I P + V I++H +F+ E++ QI L K
Sbjct: 5001 LNKTWKERVEKLDEAMQAAVQYQDGLQALYDWVDIATLKVESMPPIGTDLVTVKQQIVELKQFKTDTYQQQIELERLNHQKELLLKKVTDENDKQAVEDP--------LSELKLLWNSLEDKIINRQHKLEGALLALGPFQHALDELTTWMTHTEGLLNEQKSVGGDPKAIEIELAKHHVLQNDVLAHKSTVETVKKAGNDLIESSAGEEASSLNSRLESLNQRWQIILDKTEKRMKLLDSALFQAQGFHSEIEDMHQWLTDIERQLLASKPVGGLPETAREQLSAHLELCSLFEVKEEIYKRLMQKSQQM----------MERCTDDPEANIDQDVINLKEKWESVDTKLSERKAKLEEALEVATGFHNSLQDFI----NWLTQAEQTLTIASPPSLILDTVLFQIDEHKVFVTEVNSHRDQIMELDK 5407
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Xenopus
Match: rab11fip2 (RAB11 family interacting protein 2 (class I) [Source:Xenbase;Acc:XB-GENE-5947669]) HSP 1 Score: 264.233 bits (674), Expect = 9.579e-70 Identity = 189/682 (27.71%), Postives = 345/682 (50.59%), Query Frame = 3
Query: 4122 IQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIEN---FKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLK--LRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKS-LPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFK--LQNPSSFHKAPTKRYHDRVEFYKNIW-RNSKDSLNKSTDDLS---TNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYR-------FGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRI 6110
+ +R ER R+ + A + K F +++ L WL+ES +++ + P K+ L K K K L ++ + T R G LK+K T+ D + L +ML +R KW+ + + ++ L L+ +G DA++ + W+ + D + GD+D V LID + + E+ + LK R L E + S ++ ++E+ ++ N + L+E+L A+ F++ ++ L WL AE+ L+ LP +E L LIE+H+ +++V+E ++ + ++G+ IL CHP + + I +R+ ++ + ++ ++L +L L L+E L+ + + + + +IP D+ V +I +H IF+ E+++ + + K +K +++ + + P ++ + W R+ SL S S N R+N L +++ ++W L++E + L + L L E + FDF+ W+K ++ ++ K R+ D FRR + G +EF I+ S FPT++ + V +IF+++ + I Y +F+ +L P + + K I DK I D + ++VA C C R+ V++ G+NKYR FG + +L +VR L STVMVR+GGGW + EFL KNDPCR+
Sbjct: 6056 VVQRSLERGRMLDDARKRSKQFHEAFDKLMEWLEESERSLDSELEIANDPDKIKTQLTKHKEFQKTLGGKHTVYDTTTRSGRSLKEKATLTD-DIQKLDDMLSEIRDKWDTVCGKSIERQNKLEEALLISGQFTDALQALIDWLYRIEPQLAEDQPVHGDIDIVMNLIDNHKVFQKELGKRSSSIQALKRSARELIEGSRDDSSWVKAQMQELGTRWETVCNLSVSKQTRLEEALHQAESFHAAVHALLEWLADAEQALRFHGILP--DDEDSLRLLIEQHKEFMRRVEEKRSELNKGASMGEAILAICHPDSITTIKHWITIIRARFEEVLTWTKQHQQRLAVALDDLIAKKELLESLLAWLQWSEMTLNEKDKEDIPQDLDEVKALIEEHQIFMEEMTQKQPDVDKVTKTYKRKVESTTGQSQIPV------LDKGRGGWKRSPTPSLYPSGTQTSLETKNPRVNLLVSKWQQVWLLALERRRKLNDALDRLEELREFANFDFDVWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIEGILSSKFPTSRLEMSAVADIFDRDGDGYIDYYEFVAALHPNKDAY--KPITDADK------IEDEVTRQVAKCKCAKRFQVEQIGENKYRVISLLFKFGDSQQLRLVRILRSTVMVRVGGGWMALDEFLVKNDPCRV 6720 HSP 2 Score: 65.855 bits (159), Expect = 1.253e-9 Identity = 83/429 (19.35%), Postives = 177/429 (41.26%), Query Frame = 3
Query: 4122 IQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIENFKDQPTKVL---DHLDKLKNL-----CKQLSCQYANFH--CTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLKLR---MLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLL----KIKSLPKFQNEA-----DLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCD--MQNVNDIINKHLIFINELSEIDSQIKSLIK 5336
+ K +ER+ ++A + + D L W+D + + +E+ T ++ + +LK +Q+ + N L+ + DK VEDP L L+ WN + I+ + L L+ G A+ + W+ + + GD +E+ + + H+ +N++ HK + +K ++ E S + + +E + ++ + ++ ++R K L +L A+ F+S++ ++ WL E+ L + LP+ E +L L E E + K++ + M + C +++ + +W ++++ +R KL E+L G +++ I L + + + I P + V I++H +F+ E++ QI L K
Sbjct: 5619 LNKTWKERVEKLDEAMQAAVQYQDGLQALYDWVDIATLKVESMPPIGTDLVTVKQQIVELKQFKTDTYQQQIELERLNHQKELLLKKVTDENDKQAVEDP--------LSELKLLWNSLEDKIINRQHKLEGALLALGPFQHALDELTTWMTHTEGLLNEQKSVGGDPKAIEIELAKHHVLQNDVLAHKSTVETVKKAGNDLIESSAGEEASSLNSRLESLNQRWQIILDKTEKRMKLLDSALFQAQGFHSEIEDMHQWLTDIERQLLASKPVGGLPETAREQLSAHLELCSLFEVKEEIYKRLMQKSQQM----------MERCTDDPEANIDQDVINLKEKWESVDTKLSERKAKLEEALEVATGFHNSLQDFI----NWLTQAEQTLTIASPPSLILDTVLFQIDEHKVFVTEVNSHRDQIMELDK 6025
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Xenopus
Match: rab11fip2 (RAB11 family interacting protein 2 (class I) [Source:Xenbase;Acc:XB-GENE-5947669]) HSP 1 Score: 262.692 bits (670), Expect = 3.035e-69 Identity = 189/681 (27.75%), Postives = 344/681 (50.51%), Query Frame = 3
Query: 4122 IQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIEN---FKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLK--LRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKS-LPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFK--LQNPSSFHKAPTKRYHDRVEFYKNIW-RNSKDSLNKSTDDLS---TNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYR-------FGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCR 6107
+ +R ER R+ + A + K F +++ L WL+ES +++ + P K+ L K K K L ++ + T R G LK+K T+ D + L +ML +R KW+ + + ++ L L+ +G DA++ + W+ + D + GD+D V LID + + E+ + LK R L E + S ++ ++E+ ++ N + L+E+L A+ F++ ++ L WL AE+ L+ LP +E L LIE+H+ +++V+E ++ + ++G+ IL CHP + + I +R+ ++ + ++ ++L +L L L+E L+ + + + + +IP D+ V +I +H IF+ E+++ + + K +K +++ + + P ++ + W R+ SL S S N R+N L +++ ++W L++E + L + L L E + FDF+ W+K ++ ++ K R+ D FRR + G +EF I+ S FPT++ + V +IF+++ + I Y +F+ +L P + + K I DK I D + ++VA C C R+ V++ G+NKYR FG + +L +VR L STVMVR+GGGW + EFL KNDPCR
Sbjct: 6052 VVQRSLERGRMLDDARKRSKQFHEAFDKLMEWLEESERSLDSELEIANDPDKIKTQLTKHKEFQKTLGGKHTVYDTTTRSGRSLKEKATLTD-DIQKLDDMLSEIRDKWDTVCGKSIERQNKLEEALLISGQFTDALQALIDWLYRIEPQLAEDQPVHGDIDIVMNLIDNHKVFQKELGKRSSSIQALKRSARELIEGSRDDSSWVKAQMQELGTRWETVCNLSVSKQTRLEEALHQAESFHAAVHALLEWLADAEQALRFHGILPD--DEDSLRLLIEQHKEFMRRVEEKRSELNKGASMGEAILAICHPDSITTIKHWITIIRARFEEVLTWTKQHQQRLAVALDDLIAKKELLESLLAWLQWSEMTLNEKDKEDIPQDLDEVKALIEEHQIFMEEMTQKQPDVDKVTKTYKRKVESTTGQSQIPV------LDKGRGGWKRSPTPSLYPSGTQTSLETKNPRVNLLVSKWQQVWLLALERRRKLNDALDRLEELREFANFDFDVWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIEGILSSKFPTSRLEMSAVADIFDRDGDGYIDYYEFVAALHPNKDAY--KPITDADK------IEDEVTRQVAKCKCAKRFQVEQIGENKYRVISLLFKFGDSQQLRLVRILRSTVMVRVGGGWMALDEFLVKNDPCR 6715 HSP 2 Score: 65.855 bits (159), Expect = 1.329e-9 Identity = 83/430 (19.30%), Postives = 174/430 (40.47%), Query Frame = 3
Query: 4122 IQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIENFKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLK-----------DKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLKLR---MLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLL----KIKSLPKFQNEA-----DLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCD--MQNVNDIINKHLIFINELSEIDSQIKSLIK 5336
+ K +ER+ ++A + + D L W+D + + +E+ T ++ ++ L KQ L ++ K DK VEDP L L+ WN + I+ + L L+ G A+ + W+ + + GD +E+ + + H+ +N++ HK + +K ++ E S + + +E + ++ + ++ ++R K L +L A+ F+S++ ++ WL E+ L + LP+ E +L L E E + K++ + M + C +++ + +W ++++ +R KL E+L G +++ I L + + + I P + V I++H +F+ E++ QI L K
Sbjct: 5615 LNKTWKERVEKLDEAMQAAVQYQDGLQALYDWVDIATLKVESMPPIGTDLVTVKQQIVEL-KQFKTDTYQQQIELERLNHQKELLLKKVTDENDKQAVEDP--------LSELKLLWNSLEDKIINRQHKLEGALLALGPFQHALDELTTWMTHTEGLLNEQKSVGGDPKAIEIELAKHHVLQNDVLAHKSTVETVKKAGNDLIESSAGEEASSLNSRLESLNQRWQIILDKTEKRMKLLDSALFQAQGFHSEIEDMHQWLTDIERQLLASKPVGGLPETAREQLSAHLELCSLFEVKEEIYKRLMQKSQQM----------MERCTDDPEANIDQDVINLKEKWESVDTKLSERKAKLEEALEVATGFHNSLQDFI----NWLTQAEQTLTIASPPSLILDTVLFQIDEHKVFVTEVNSHRDQIMELDK 6021
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Mouse
Match: Dst (dystonin [Source:MGI Symbol;Acc:MGI:104627]) HSP 1 Score: 268.085 bits (684), Expect = 1.273e-71 Identity = 193/694 (27.81%), Postives = 347/694 (50.00%), Query Frame = 3
Query: 4119 KIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIEN---FKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLK--LRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKI-KSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFKLQ--NPSSFH---------KAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYR------FGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRIANSLDLQ 6131
K+ +RL ER R ++A + K F ++++ L WL+ES +++ + P K+ L + K K L +++ + T R G LK+K ++ D + L ML LR KW+ I V ++ L L+ +G DA++ + W+ + D + GD+D V LID + + E+ + LK R L E + S +R ++E+ ++ + L+ +LQ A+EF+S ++ L WL +AE+ L+ +LP +E L LIE+H+ +K+++E + ++ +G +L CHP + + I +R+ ++ + ++ ++L +L L L+E L+ + + + + IP +++ V +I +H F+ E++ + + K +K + +P S +A KR+ FY + T + N R+N L +++ ++W L++E + L + L L E + FDF+ W+K ++ ++ K R+ D FRR + G +EF + I+ S FPT++ + V +IF+++ + I Y +F+ +L P + + K I DK I D + ++VA C C R+ V++ GDNKYR FG + +L +VR L STVMVR+GGGW + EFL KNDPCR +++
Sbjct: 741 KVVQRLVERGRSLDEARKRAKQFHEAWSKLMEWLEESEKSLDSELEIANDPDKIKAQLVQHKEFQKSLGGKHSVYDTTNRTGRSLKEKTSLAD-DNLKLDNMLSELRDKWDTICGKSVERQNKLEEALLFSGQFTDALQALIDWLYRVEPQLAEDQPVHGDIDLVMNLIDNHKVFQKELGKRTSSVQALKRSARELIEGSRDDSSWVRVQMQELSTRWETVCALSISKQTRLESALQQAEEFHSVVHTLLEWLAEAEQTLRFHGALP--DDEDALRTLIEQHKEFMKRLEEKRAELSKATGMGDALLAVCHPDSITTIKHWITIIQARFEEVLAWAKQHQQRLAGALAGLIAKQELLETLLAWLQWAETTLTEKDKEVIPQEIEEVKTLIAEHQTFMEEMTRKQPDVDKVTKTYKRRATDPPSLQSHIPVLDKGRAGRKRF-PASGFYPS---------GSQTQIETKNPRVNLLVSKWQQVWLLALERRRKLNDALDRLEELREFANFDFDIWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILSSKFPTSRLEMSAVADIFDRDGDGYIDYYEFVAALHPNKDAY--KPITDADK------IEDEVTRQVAKCKCAKRFQVEQIGDNKYRFFLGNQFGDSQQLRLVRILRSTVMVRVGGGWMALDEFLVKNDPCRAKGRTNME 1413 HSP 2 Score: 56.6102 bits (135), Expect = 4.502e-7 Identity = 54/262 (20.61%), Postives = 111/262 (42.37%), Query Frame = 3
Query: 4368 DKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLKLR---MLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEK-LLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESL 5141
DK TV+DP L L+ W+ + IV+ + L L+ G A+ + W+ + + GD +E+ + + H+ +N++ H+ + + ++ + E S+++ + + ++ + QR + L +L+ AK F+ ++ +L WL E+ LL K L A E + H + + T + G+ +L C A +++ I +W + S+ ++ KL E+L
Sbjct: 397 DKHTVQDP--------LMELKLIWDSLDERIVSRQHKLEGALLALGQFQHALDELLAWLTHTKGLLSEQKPVGGDPKAIEIELAKHHVLQNDVLAHQSTVEAVNKAGNDLIESSEGEEASNLQYKLRILNQRWQDILEKTDQRKQQLDSALRQAKGFHGEIEDLQQWLTDTERHLLASKPLGGLPETAK--EQLNAHMEVCTAFAIKEETYKSLMLRGQQMLARCPRSAETNIDQDITNLKEKWESVKSKLNEKKTKLEEAL 648 HSP 3 Score: 53.9138 bits (128), Expect = 3.393e-6 Identity = 104/564 (18.44%), Postives = 243/564 (43.09%), Query Frame = 3
Query: 3663 DLNKQRNSIENSLKLASSHIESCQYDKCTFLSTAIKTLFHKYKRCQHLAAKRKEKLSIYSKLILEYDADKQTLQKDILELENLSIKRMNPSKITVTCAEQIKS-IKISIQTADKNFDIYEHLTIRSFDI--KCFGNRIDN-SQLILELKEYGLKLAKIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIENFKDQPTKVLD----HLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLE-MLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEV-LIDECHMTENEINNHK-EDFKNLKLRMLNEIKNYEESDIR--NTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSLPKFQNEADLV-ELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIE--IPCDMQNVNDIINKHLIFINELSE 5306
D+ ++++E K + IES + ++ + L ++ L +++ +RK++L + + + + LQ+ + + E + + T EQ+ + +++ A K + Y+ L +R + +C + N Q I LKE K ++ +L E+ +A F +S + WL ++ + N +P+ +LD +D+ K +++ + G++LK +D +L++ +L +++++W K+ +V +L+ + ++A + W+ +S +L++ D D ++ L+ ++ H D N R L E + + +++ N + E+ +++ +R L+E+L + +F L L WL + E L + P + DLV LI+ H+ K++ + +++ + ++ +++V + + +RW + + S + +L +L +++ L+ E L + + +R +P D + +I +H F+ L E
Sbjct: 480 DVLAHQSTVEAVNKAGNDLIESSEGEEASNLQYKLRILNQRWQDILEKTDQRKQQLDSALRQAKGFHGEIEDLQQWLTDTERHLLASKPLGGLPETAKEQLNAHMEVCTAFAIKE-ETYKSLMLRGQQMLARCPRSAETNIDQDITNLKE---KWESVKSKLNEKKTKLEEALHLAMNFHNSLQDFINWLTQAEQTL-NVASRPSLILDTILFQIDEHKVFANEVNSHREQIIELDKTGTHLKYFSQKQD---VVLIKNLLISVQSRWEKVVQRLVERGRSLDEARKRAKQFHEAWSKLMEWLEESEKSLDSELEIANDPDKIKAQLVQHKEFQKSLGGKHSVYDTTNRTGRSLKEKTSLADDNLKLDNMLSELRDKWDTICGKSVERQNKLEEALLFSGQFTDALQALIDWLYRVEPQL-AEDQP-VHGDIDLVMNLIDNHKVFQKELGKRTSSVQALKRSARELIEGSRD-DSSWVRVQMQELSTRWETVCALSISKQTRLESALQQAEEFHSVVHTLL----EWLAEAEQTLRFHGALPDDEDALRTLIEQHKEFMKRLEE 1028
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Mouse
Match: Dst (dystonin [Source:MGI Symbol;Acc:MGI:104627]) HSP 1 Score: 268.47 bits (685), Expect = 3.953e-71 Identity = 193/687 (28.09%), Postives = 345/687 (50.22%), Query Frame = 3
Query: 4119 KIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIEN---FKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLK--LRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKI-KSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFKLQ--NPSSFH---------KAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYR------FGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRI 6110
K+ +RL ER R ++A + K F ++++ L WL+ES +++ + P K+ L + K K L +++ + T R G LK+K ++ D + L ML LR KW+ I V ++ L L+ +G DA++ + W+ + D + GD+D V LID + + E+ + LK R L E + S +R ++E+ ++ + L+ +LQ A+EF+S ++ L WL +AE+ L+ +LP +E L LIE+H+ +K+++E + ++ +G +L CHP + + I +R+ ++ + ++ ++L +L L L+E L+ + + + + IP +++ V +I +H F+ E++ + + K +K + +P S +A KR+ FY + T + N R+N L +++ ++W L++E + L + L L E + FDF+ W+K ++ ++ K R+ D FRR + G +EF + I+ S FPT++ + V +IF+++ + I Y +F+ +L P + + K I DK I D + ++VA C C R+ V++ GDNKYR FG + +L +VR L STVMVR+GGGW + EFL KNDPCR+
Sbjct: 4495 KVVQRLVERGRSLDEARKRAKQFHEAWSKLMEWLEESEKSLDSELEIANDPDKIKAQLVQHKEFQKSLGGKHSVYDTTNRTGRSLKEKTSLAD-DNLKLDNMLSELRDKWDTICGKSVERQNKLEEALLFSGQFTDALQALIDWLYRVEPQLAEDQPVHGDIDLVMNLIDNHKVFQKELGKRTSSVQALKRSARELIEGSRDDSSWVRVQMQELSTRWETVCALSISKQTRLESALQQAEEFHSVVHTLLEWLAEAEQTLRFHGALPD--DEDALRTLIEQHKEFMKRLEEKRAELSKATGMGDALLAVCHPDSITTIKHWITIIQARFEEVLAWAKQHQQRLAGALAGLIAKQELLETLLAWLQWAETTLTEKDKEVIPQEIEEVKTLIAEHQTFMEEMTRKQPDVDKVTKTYKRRATDPPSLQSHIPVLDKGRAGRKRF-PASGFYPS---------GSQTQIETKNPRVNLLVSKWQQVWLLALERRRKLNDALDRLEELREFANFDFDIWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILSSKFPTSRLEMSAVADIFDRDGDGYIDYYEFVAALHPNKDAY--KPITDADK------IEDEVTRQVAKCKCAKRFQVEQIGDNKYRFFLGNQFGDSQQLRLVRILRSTVMVRVGGGWMALDEFLVKNDPCRV 5160 HSP 2 Score: 56.225 bits (134), Expect = 7.681e-7 Identity = 54/262 (20.61%), Postives = 111/262 (42.37%), Query Frame = 3
Query: 4368 DKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLKLR---MLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEK-LLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESL 5141
DK TV+DP L L+ W+ + IV+ + L L+ G A+ + W+ + + GD +E+ + + H+ +N++ H+ + + ++ + E S+++ + + ++ + QR + L +L+ AK F+ ++ +L WL E+ LL K L A E + H + + T + G+ +L C A +++ I +W + S+ ++ KL E+L
Sbjct: 4151 DKHTVQDP--------LMELKLIWDSLDERIVSRQHKLEGALLALGQFQHALDELLAWLTHTKGLLSEQKPVGGDPKAIEIELAKHHVLQNDVLAHQSTVEAVNKAGNDLIESSEGEEASNLQYKLRILNQRWQDILEKTDQRKQQLDSALRQAKGFHGEIEDLQQWLTDTERHLLASKPLGGLPETAK--EQLNAHMEVCTAFAIKEETYKSLMLRGQQMLARCPRSAETNIDQDITNLKEKWESVKSKLNEKKTKLEEAL 4402
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Mouse
Match: Dst (dystonin [Source:MGI Symbol;Acc:MGI:104627]) HSP 1 Score: 267.7 bits (683), Expect = 5.790e-71 Identity = 193/687 (28.09%), Postives = 345/687 (50.22%), Query Frame = 3
Query: 4119 KIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIEN---FKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLK--LRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKI-KSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFKLQ--NPSSFH---------KAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYR------FGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRI 6110
K+ +RL ER R ++A + K F ++++ L WL+ES +++ + P K+ L + K K L +++ + T R G LK+K ++ D + L ML LR KW+ I V ++ L L+ +G DA++ + W+ + D + GD+D V LID + + E+ + LK R L E + S +R ++E+ ++ + L+ +LQ A+EF+S ++ L WL +AE+ L+ +LP +E L LIE+H+ +K+++E + ++ +G +L CHP + + I +R+ ++ + ++ ++L +L L L+E L+ + + + + IP +++ V +I +H F+ E++ + + K +K + +P S +A KR+ FY + T + N R+N L +++ ++W L++E + L + L L E + FDF+ W+K ++ ++ K R+ D FRR + G +EF + I+ S FPT++ + V +IF+++ + I Y +F+ +L P + + K I DK I D + ++VA C C R+ V++ GDNKYR FG + +L +VR L STVMVR+GGGW + EFL KNDPCR+
Sbjct: 6509 KVVQRLVERGRSLDEARKRAKQFHEAWSKLMEWLEESEKSLDSELEIANDPDKIKAQLVQHKEFQKSLGGKHSVYDTTNRTGRSLKEKTSLAD-DNLKLDNMLSELRDKWDTICGKSVERQNKLEEALLFSGQFTDALQALIDWLYRVEPQLAEDQPVHGDIDLVMNLIDNHKVFQKELGKRTSSVQALKRSARELIEGSRDDSSWVRVQMQELSTRWETVCALSISKQTRLESALQQAEEFHSVVHTLLEWLAEAEQTLRFHGALPD--DEDALRTLIEQHKEFMKRLEEKRAELSKATGMGDALLAVCHPDSITTIKHWITIIQARFEEVLAWAKQHQQRLAGALAGLIAKQELLETLLAWLQWAETTLTEKDKEVIPQEIEEVKTLIAEHQTFMEEMTRKQPDVDKVTKTYKRRATDPPSLQSHIPVLDKGRAGRKRF-PASGFYPS---------GSQTQIETKNPRVNLLVSKWQQVWLLALERRRKLNDALDRLEELREFANFDFDIWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILSSKFPTSRLEMSAVADIFDRDGDGYIDYYEFVAALHPNKDAY--KPITDADK------IEDEVTRQVAKCKCAKRFQVEQIGDNKYRFFLGNQFGDSQQLRLVRILRSTVMVRVGGGWMALDEFLVKNDPCRV 7174 HSP 2 Score: 56.6102 bits (135), Expect = 5.605e-7 Identity = 55/262 (20.99%), Postives = 110/262 (41.98%), Query Frame = 3
Query: 4368 DKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHK---EDFKNLKLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEK-LLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESL 5141
DK TV+DP L L+ W+ + IV+ + L L+ G A+ + W+ + + GD +E+ + + H+ +N++ H+ E ++ + E S+++ + + ++ + QR + L +L+ AK F+ ++ +L WL E+ LL K L A E + H + + T + G+ +L C A +++ I +W + S+ ++ KL E+L
Sbjct: 6165 DKHTVQDP--------LMELKLIWDSLDERIVSRQHKLEGALLALGQFQHALDELLAWLTHTKGLLSEQKPVGGDPKAIEIELAKHHVLQNDVLAHQSTVEAVNKAGNDLIESSEGEEASNLQYKLRILNQRWQDILEKTDQRKQQLDSALRQAKGFHGEIEDLQQWLTDTERHLLASKPLGGLPETAK--EQLNAHMEVCTAFAIKEETYKSLMLRGQQMLARCPRSAETNIDQDITNLKEKWESVKSKLNEKKTKLEEAL 6416
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Mouse
Match: Dst (dystonin [Source:MGI Symbol;Acc:MGI:104627]) HSP 1 Score: 267.314 bits (682), Expect = 9.757e-71 Identity = 193/687 (28.09%), Postives = 345/687 (50.22%), Query Frame = 3
Query: 4119 KIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIEN---FKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLK--LRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKI-KSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFKLQ--NPSSFH---------KAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYR------FGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRI 6110
K+ +RL ER R ++A + K F ++++ L WL+ES +++ + P K+ L + K K L +++ + T R G LK+K ++ D + L ML LR KW+ I V ++ L L+ +G DA++ + W+ + D + GD+D V LID + + E+ + LK R L E + S +R ++E+ ++ + L+ +LQ A+EF+S ++ L WL +AE+ L+ +LP +E L LIE+H+ +K+++E + ++ +G +L CHP + + I +R+ ++ + ++ ++L +L L L+E L+ + + + + IP +++ V +I +H F+ E++ + + K +K + +P S +A KR+ FY + T + N R+N L +++ ++W L++E + L + L L E + FDF+ W+K ++ ++ K R+ D FRR + G +EF + I+ S FPT++ + V +IF+++ + I Y +F+ +L P + + K I DK I D + ++VA C C R+ V++ GDNKYR FG + +L +VR L STVMVR+GGGW + EFL KNDPCR+
Sbjct: 6796 KVVQRLVERGRSLDEARKRAKQFHEAWSKLMEWLEESEKSLDSELEIANDPDKIKAQLVQHKEFQKSLGGKHSVYDTTNRTGRSLKEKTSLAD-DNLKLDNMLSELRDKWDTICGKSVERQNKLEEALLFSGQFTDALQALIDWLYRVEPQLAEDQPVHGDIDLVMNLIDNHKVFQKELGKRTSSVQALKRSARELIEGSRDDSSWVRVQMQELSTRWETVCALSISKQTRLESALQQAEEFHSVVHTLLEWLAEAEQTLRFHGALPD--DEDALRTLIEQHKEFMKRLEEKRAELSKATGMGDALLAVCHPDSITTIKHWITIIQARFEEVLAWAKQHQQRLAGALAGLIAKQELLETLLAWLQWAETTLTEKDKEVIPQEIEEVKTLIAEHQTFMEEMTRKQPDVDKVTKTYKRRATDPPSLQSHIPVLDKGRAGRKRF-PASGFYPS---------GSQTQIETKNPRVNLLVSKWQQVWLLALERRRKLNDALDRLEELREFANFDFDIWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILSSKFPTSRLEMSAVADIFDRDGDGYIDYYEFVAALHPNKDAY--KPITDADK------IEDEVTRQVAKCKCAKRFQVEQIGDNKYRFFLGNQFGDSQQLRLVRILRSTVMVRVGGGWMALDEFLVKNDPCRV 7461 HSP 2 Score: 56.225 bits (134), Expect = 7.340e-7 Identity = 55/262 (20.99%), Postives = 110/262 (41.98%), Query Frame = 3
Query: 4368 DKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHK---EDFKNLKLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEK-LLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESL 5141
DK TV+DP L L+ W+ + IV+ + L L+ G A+ + W+ + + GD +E+ + + H+ +N++ H+ E ++ + E S+++ + + ++ + QR + L +L+ AK F+ ++ +L WL E+ LL K L A E + H + + T + G+ +L C A +++ I +W + S+ ++ KL E+L
Sbjct: 6452 DKHTVQDP--------LMELKLIWDSLDERIVSRQHKLEGALLALGQFQHALDELLAWLTHTKGLLSEQKPVGGDPKAIEIELAKHHVLQNDVLAHQSTVEAVNKAGNDLIESSEGEEASNLQYKLRILNQRWQDILEKTDQRKQQLDSALRQAKGFHGEIEDLQQWLTDTERHLLASKPLGGLPETAK--EQLNAHMEVCTAFAIKEETYKSLMLRGQQMLARCPRSAETNIDQDITNLKEKWESVKSKLNEKKTKLEEAL 6703
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Mouse
Match: Dst (dystonin [Source:MGI Symbol;Acc:MGI:104627]) HSP 1 Score: 266.929 bits (681), Expect = 9.864e-71 Identity = 193/694 (27.81%), Postives = 347/694 (50.00%), Query Frame = 3
Query: 4119 KIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIEN---FKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLK--LRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIK-SLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFKLQ--NPSSFH---------KAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYR------FGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRIANSLDLQ 6131
K+ +RL ER R ++A + K F ++++ L WL+ES +++ + P K+ L + K K L +++ + T R G LK+K ++ D + L ML LR KW+ I V ++ L L+ +G DA++ + W+ + D + GD+D V LID + + E+ + LK R L E + S +R ++E+ ++ + L+ +LQ A+EF+S ++ L WL +AE+ L+ +LP +E L LIE+H+ +K+++E + ++ +G +L CHP + + I +R+ ++ + ++ ++L +L L L+E L+ + + + + IP +++ V +I +H F+ E++ + + K +K + +P S +A KR+ FY + T + N R+N L +++ ++W L++E + L + L L E + FDF+ W+K ++ ++ K R+ D FRR + G +EF + I+ S FPT++ + V +IF+++ + I Y +F+ +L P + + K I DK I D + ++VA C C R+ V++ GDNKYR FG + +L +VR L STVMVR+GGGW + EFL KNDPCR +++
Sbjct: 4278 KVVQRLVERGRSLDEARKRAKQFHEAWSKLMEWLEESEKSLDSELEIANDPDKIKAQLVQHKEFQKSLGGKHSVYDTTNRTGRSLKEKTSLAD-DNLKLDNMLSELRDKWDTICGKSVERQNKLEEALLFSGQFTDALQALIDWLYRVEPQLAEDQPVHGDIDLVMNLIDNHKVFQKELGKRTSSVQALKRSARELIEGSRDDSSWVRVQMQELSTRWETVCALSISKQTRLESALQQAEEFHSVVHTLLEWLAEAEQTLRFHGALPD--DEDALRTLIEQHKEFMKRLEEKRAELSKATGMGDALLAVCHPDSITTIKHWITIIQARFEEVLAWAKQHQQRLAGALAGLIAKQELLETLLAWLQWAETTLTEKDKEVIPQEIEEVKTLIAEHQTFMEEMTRKQPDVDKVTKTYKRRATDPPSLQSHIPVLDKGRAGRKRF-PASGFYPS---------GSQTQIETKNPRVNLLVSKWQQVWLLALERRRKLNDALDRLEELREFANFDFDIWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILSSKFPTSRLEMSAVADIFDRDGDGYIDYYEFVAALHPNKDAY--KPITDADK------IEDEVTRQVAKCKCAKRFQVEQIGDNKYRFFLGNQFGDSQQLRLVRILRSTVMVRVGGGWMALDEFLVKNDPCRAKGRTNME 4950 HSP 2 Score: 55.4546 bits (132), Expect = 1.206e-6 Identity = 54/262 (20.61%), Postives = 111/262 (42.37%), Query Frame = 3
Query: 4368 DKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLKLR---MLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEK-LLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESL 5141
DK TV+DP L L+ W+ + IV+ + L L+ G A+ + W+ + + GD +E+ + + H+ +N++ H+ + + ++ + E S+++ + + ++ + QR + L +L+ AK F+ ++ +L WL E+ LL K L A E + H + + T + G+ +L C A +++ I +W + S+ ++ KL E+L
Sbjct: 3934 DKHTVQDP--------LMELKLIWDSLDERIVSRQHKLEGALLALGQFQHALDELLAWLTHTKGLLSEQKPVGGDPKAIEIELAKHHVLQNDVLAHQSTVEAVNKAGNDLIESSEGEEASNLQYKLRILNQRWQDILEKTDQRKQQLDSALRQAKGFHGEIEDLQQWLTDTERHLLASKPLGGLPETAK--EQLNAHMEVCTAFAIKEETYKSLMLRGQQMLARCPRSAETNIDQDITNLKEKWESVKSKLNEKKTKLEEAL 4185
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. UniProt/SwissProt
Match: sp|Q91ZU6|DYST_MOUSE (Dystonin OS=Mus musculus OX=10090 GN=Dst PE=1 SV=2) HSP 1 Score: 267.7 bits (683), Expect = 4.050e-70 Identity = 193/687 (28.09%), Postives = 345/687 (50.22%), Query Frame = 3
Query: 4119 KIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIEN---FKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLK--LRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKI-KSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFKLQ--NPSSFH---------KAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYR------FGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRI 6110
K+ +RL ER R ++A + K F ++++ L WL+ES +++ + P K+ L + K K L +++ + T R G LK+K ++ D + L ML LR KW+ I V ++ L L+ +G DA++ + W+ + D + GD+D V LID + + E+ + LK R L E + S +R ++E+ ++ + L+ +LQ A+EF+S ++ L WL +AE+ L+ +LP +E L LIE+H+ +K+++E + ++ +G +L CHP + + I +R+ ++ + ++ ++L +L L L+E L+ + + + + IP +++ V +I +H F+ E++ + + K +K + +P S +A KR+ FY + T + N R+N L +++ ++W L++E + L + L L E + FDF+ W+K ++ ++ K R+ D FRR + G +EF + I+ S FPT++ + V +IF+++ + I Y +F+ +L P + + K I DK I D + ++VA C C R+ V++ GDNKYR FG + +L +VR L STVMVR+GGGW + EFL KNDPCR+
Sbjct: 6509 KVVQRLVERGRSLDEARKRAKQFHEAWSKLMEWLEESEKSLDSELEIANDPDKIKAQLVQHKEFQKSLGGKHSVYDTTNRTGRSLKEKTSLAD-DNLKLDNMLSELRDKWDTICGKSVERQNKLEEALLFSGQFTDALQALIDWLYRVEPQLAEDQPVHGDIDLVMNLIDNHKVFQKELGKRTSSVQALKRSARELIEGSRDDSSWVRVQMQELSTRWETVCALSISKQTRLESALQQAEEFHSVVHTLLEWLAEAEQTLRFHGALPD--DEDALRTLIEQHKEFMKRLEEKRAELSKATGMGDALLAVCHPDSITTIKHWITIIQARFEEVLAWAKQHQQRLAGALAGLIAKQELLETLLAWLQWAETTLTEKDKEVIPQEIEEVKTLIAEHQTFMEEMTRKQPDVDKVTKTYKRRATDPPSLQSHIPVLDKGRAGRKRF-PASGFYPS---------GSQTQIETKNPRVNLLVSKWQQVWLLALERRRKLNDALDRLEELREFANFDFDIWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILSSKFPTSRLEMSAVADIFDRDGDGYIDYYEFVAALHPNKDAY--KPITDADK------IEDEVTRQVAKCKCAKRFQVEQIGDNKYRFFLGNQFGDSQQLRLVRILRSTVMVRVGGGWMALDEFLVKNDPCRV 7174 HSP 2 Score: 56.6102 bits (135), Expect = 3.921e-6 Identity = 55/262 (20.99%), Postives = 110/262 (41.98%), Query Frame = 3
Query: 4368 DKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHK---EDFKNLKLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEK-LLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESL 5141
DK TV+DP L L+ W+ + IV+ + L L+ G A+ + W+ + + GD +E+ + + H+ +N++ H+ E ++ + E S+++ + + ++ + QR + L +L+ AK F+ ++ +L WL E+ LL K L A E + H + + T + G+ +L C A +++ I +W + S+ ++ KL E+L
Sbjct: 6165 DKHTVQDP--------LMELKLIWDSLDERIVSRQHKLEGALLALGQFQHALDELLAWLTHTKGLLSEQKPVGGDPKAIEIELAKHHVLQNDVLAHQSTVEAVNKAGNDLIESSEGEEASNLQYKLRILNQRWQDILEKTDQRKQQLDSALRQAKGFHGEIEDLQQWLTDTERHLLASKPLGGLPETAK--EQLNAHMEVCTAFAIKEETYKSLMLRGQQMLARCPRSAETNIDQDITNLKEKWESVKSKLNEKKTKLEEAL 6416
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. UniProt/SwissProt
Match: sp|D3ZHV2|MACF1_RAT (Microtubule-actin cross-linking factor 1 OS=Rattus norvegicus OX=10116 GN=Macf1 PE=1 SV=1) HSP 1 Score: 259.61 bits (662), Expect = 1.241e-67 Identity = 188/679 (27.69%), Postives = 334/679 (49.19%), Query Frame = 3
Query: 4119 KIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIEN---FKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLKL--RMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFKLQNPSSFHKAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDL--------STNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPER---RSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYRFGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCR 6107
K+ +R ER R + A + K F +++ L WL+++ +++ + P K+ L K K K L + + T+R G LK+K + D + L +L +R KW+ + V + L L+ +G DA++ + W+ + D + GDLD V L+D + + E+ + LK R L E + + ++ ++E+ ++ + L+++L+ A+EF ++ L WL +AE+ L+ + EA L LI+ H+ +K+V+E + + +G+VIL CHP + I +R+ ++ + ++ ++L +L L + L+EEL+ + + + IP ++ V +I +H F+ E++ + + K +K +N H AP F + S+ SLN+ T + N R+N L+ ++ ++W L++E + L + L L E ++ FDF+ W+K ++ ++ K R+ D FRR + G +EF + I+ S FPT K + V +IF+++ + I Y +F+ +L P + R D DK I D + ++VA C C R+ V++ G+NKYRFG + +L +VR L STVMVR+GGGW + EFL KNDPCR
Sbjct: 4576 KVVQRSIERGRSLDDARKRAKQFHEAWKKLIDWLEDAESHLDSELEISNDPDKIKLQLSKHKEFQKTLGGKQPVYDTTIRTGRALKEKTLLAD-DAQKLDNLLGEVRDKWDTVCGKSVERQHKLEEALLFSGQFMDALQALVDWLYKVEPQLAEDQPVHGDLDLVMNLMDAHKVFQKELGKRTGTVQVLKRSGRELIESSRDDTTWVKGQLQELSTRWDTVCKLSVSKQSRLEQALKQAEEFRDTVHMLLEWLSEAEQTLRFRGALPDDTEA-LQSLIDTHKEFMKKVEEKRVDVNAAVAMGEVILAVCHPDCITTIKHWITIIRARFEEVLTWAKQHQQRLETALSELVANAELLEELLAWIQWAETTLIQRDQEPIPQNIDRVKALITEHQSFMEEMTRKQPDVDRVTKTYKRKNIEPTH-AP---------FIEKSRSGSRKSLNQPTPPPMPILSQSEAKNPRINQLSARWQQVWLLALERQRKLNDALDRLEELKEFANFDFDVWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILASKFPTTKLEMTAVADIFDRDGDGYIDYYEFVAALHPNKDAYRPTTDA-----DK------IEDEVTRQVAQCKCAKRFQVEQIGENKYRFGDSQQLRLVRILRSTVMVRVGGGWMALDEFLVKNDPCR 5231 HSP 2 Score: 60.8474 bits (146), Expect = 2.221e-7 Identity = 81/407 (19.90%), Postives = 169/407 (41.52%), Query Frame = 3
Query: 4137 RERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIENFKDQPT---KVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHK---EDFKNLKLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGK-VILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKI---QNEIRIEIPCD--MQNVNDIINKHLIFINELSEIDSQI 5321
+ER+ A + + D+ + WLD + + + T V D L+++K ++ Q G + K T ++ ++ I+ E L L+ W + I + L L+ G A++ + W+ + + GD +EV + + H+ +N++ H+ E +L + S +R+ +E + + + ++R + LQ +LQ A+ F+S++ E + L + E L S P E ++ H L Q++ + + G+ ++L+ + + + + +W+ ++S+ E+R KL E+L +L E + E +N + + + I P + V I +H +F NE++ QI
Sbjct: 4144 KERLEKLEDAMQAAVQYQDTLQAMFDWLDNTVIRLCTMPPVGTDLNTVKDQLNEMKEFKVEVYQQQIEMEKLNHQGELMLKKAT-DETDRDIIREPLTELKHLWENLGEKIAHRQHKLEGALLALGQFQHALEELMGWLTHTEELLDAQRPISGDPKVIEVELAKHHVLKNDVLAHQATVETVNKAGNELLESSAGDDASSLRSRLETMNQCWESVLQKTEEREQQLQSTLQQAQGFHSEIEEFLLELNRMESQLSA-SKPTGGLPETAREQLDAHMELHSQLRAKEEIYNQLLDKGRLMLLSRGDSGSGSKTEQSVALLEQKWHVVSSKVEERKSKLEEAL-------SLATEFQNSLQEFINWLTLAEQSLNIASPPSLILNTVLSQIEEHKVFANEVNAHRDQI 4541
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. UniProt/SwissProt
Match: sp|Q03001|DYST_HUMAN (Dystonin OS=Homo sapiens OX=9606 GN=DST PE=1 SV=4) HSP 1 Score: 256.144 bits (653), Expect = 1.426e-66 Identity = 190/687 (27.66%), Postives = 343/687 (49.93%), Query Frame = 3
Query: 4119 KIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIEN---FKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLK--LRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKS-LPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFKLQ--NPSSFH---------KAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYR------FGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRI 6110
K+ +RL ER R + A + K F ++++ L WL+ES +++ + P K+ L + K K L +++ + T R G LK+K ++ D + L +ML LR KW+ I V ++ L L+ +G DA++ + W+ + D + GD+D V LID + E+ + LK R L E + S ++ ++E+ ++ + L+ +L+ A+EF+S ++ L WL +AE+ L+ LP +E L LI++H+ +K+++E + + +G +L CHP + + I +R+ ++ + ++ ++L +L L L+E L+ + + ++ + IP +++ V +I +H F+ E++ + + K +K + +PSS +A KR+ Y + T + N R+N L +++ ++W L++E + L + L L E + FDF+ W+K ++ ++ K R+ D FRR + G +EF + I+ S FPT++ + V +IF+++ + I Y +F+ +L P + + K I DK I D + ++VA C C R+ V++ GDNKYR FG + +L +VR L STVMVR+GGGW + EFL KNDPCR+
Sbjct: 6687 KVVQRLVERGRSLDDARKRAKQFHEAWSKLMEWLEESEKSLDSELEIANDPDKIKTQLAQHKEFQKSLGAKHSVYDTTNRTGRSLKEKTSLAD-DNLKLDDMLSELRDKWDTICGKSVERQNKLEEALLFSGQFTDALQALIDWLYRVEPQLAEDQPVHGDIDLVMNLIDNHKAFQKELGKRTSSVQALKRSARELIEGSRDDSSWVKVQMQELSTRWETVCALSISKQTRLEAALRQAEEFHSVVHALLEWLAEAEQTLRFHGVLP--DDEDALRTLIDQHKEFMKKLEEKRAELNKATTMGDTVLAICHPDSITTIKHWITIIRARFEEVLAWAKQHQQRLASALAGLIAKQELLEALLAWLQWAETTLTDKDKEVIPQEIEEVKALIAEHQTFMEEMTRKQPDVDKVTKTYKRRAADPSSLQSHIPVLDKGRAGRKRF-PASSLYPS---------GSQTQIETKNPRVNLLVSKWQQVWLLALERRRKLNDALDRLEELREFANFDFDIWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILSSKFPTSRLEMSAVADIFDRDGDGYIDYYEFVAALHPNKDAY--KPITDADK------IEDEVTRQVAKCKCAKRFQVEQIGDNKYRFFLGNQFGDSQQLRLVRILRSTVMVRVGGGWMALDEFLVKNDPCRV 7352 HSP 2 Score: 63.929 bits (154), Expect = 2.611e-8 Identity = 56/262 (21.37%), Postives = 112/262 (42.75%), Query Frame = 3
Query: 4368 DKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHK---EDFKNLKLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEK-LLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESL 5141
DK TV+DP L L+ W+ + I+ + L L+ G A+ + W+ + + GD +E+ + + H+ +N++ H+ E ++ E S+++N +E + ++ + +QR + L +L+ AK F+ ++ +L WL E+ LL K L A E + H + + + T + G+ +L C A +++ I +W + ++ +R KL E+L
Sbjct: 6343 DKHTVQDP--------LMELKLIWDSLEERIINRQHKLEGALLALGQFQHALDELLAWLTHTEGLLSEQKPVGGDPKAIEIELAKHHVLQNDVLAHQSTVEAVNKAGNDLIESSAGEEASNLQNKLEVLNQRWQNVLEKTEQRKQQLDGALRQAKGFHGEIEDLQQWLTDTERHLLASKPLGGLPETAK--EQLNVHMEVCAAFEAKEETYKSLMQKGQQMLARCPKSAETNIDQDINNLKEKWESVETKLNERKTKLEEAL 6594
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. UniProt/SwissProt
Match: sp|Q9UPN3|MACF1_HUMAN (Microtubule-actin cross-linking factor 1, isoforms 1/2/3/5 OS=Homo sapiens OX=9606 GN=MACF1 PE=1 SV=4) HSP 1 Score: 253.062 bits (645), Expect = 1.153e-65 Identity = 182/672 (27.08%), Postives = 333/672 (49.55%), Query Frame = 3
Query: 4119 KIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIEN---FKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTV-EDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLKLRMLNEIKNYEESD--IRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFKLQNPSSFHKAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPER---RSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYRFGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCR 6107
K+ +R ER R + A + K F +++ L WL+++ +++ + P K+ L K K K L + + T+R G LK+K + ED +K L L +R KW+ + V + L L+ +G DA++ + W+ + D + GDLD V L+D + + E+ + LK I+N + ++ ++E+ ++ + L+++L+ A+ F ++ L WL +AE+ L+ + EA L LI+ H+ +K+V+E + + + +G+VIL CHP + I +R+ ++ + ++ ++L +L L + L+EEL+ + + + IP ++ V +I +H F+ E++ + + K +K +N H ++ R K++ + + + + + N R+N L+ ++ ++W L++E + L + L L E ++ FDF+ W+K ++ ++ K R+ D FRR + G +EF + I+ S FPT K + V +IF+++ + I Y +F+ +L P + R D DK I D + ++VA C C R+ V++ G+NKYRFG + +L +VR L STVMVR+GGGW + EFL KNDPCR
Sbjct: 6534 KVVQRSIERGRSLDDARKRAKQFHEAWKKLIDWLEDAESHLDSELEISNDPDKIKLQLSKHKEFQKTLGGKQPVYDTTIRTGRALKEKTLLPEDSQK--LDNFLGEVRDKWDTVCGKSVERQHKLEEALLFSGQFMDALQALVDWLYKVEPQLAEDQPVHGDLDLVMNLMDAHKVFQKELGKRTGTVQVLKRSGRELIENSRDDTTWVKGQLQELSTRWDTVCKLSVSKQSRLEQALKQAEVFRDTVHMLLEWLSEAEQTLRFRGALPDDTEA-LQSLIDTHKEFMKKVEEKRVDVNSAVAMGEVILAVCHPDCITTIKHWITIIRARFEEVLTWAKQHQQRLETALSELVANAELLEELLAWIQWAETTLIQRDQEPIPQNIDRVKALIAEHQTFMEEMTRKQPDVDRVTKTYKRKNIEPTHAPFIEK--SRSGGRKSLSQPTPPPMPILSQSEAKNPRINQLSARWQQVWLLALERQRKLNDALDRLEELKEFANFDFDVWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILASKFPTTKLEMTAVADIFDRDGDGYIDYYEFVAALHPNKDAYRPTTDA-----DK------IEDEVTRQVAQCKCAKRFQVEQIGENKYRFGDSQQLRLVRILRSTVMVRVGGGWMALDEFLVKNDPCR 7189 HSP 2 Score: 62.3882 bits (150), Expect = 6.505e-8 Identity = 82/409 (20.05%), Postives = 168/409 (41.08%), Query Frame = 3
Query: 4122 IQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIENFKDQPT---KVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHK---EDFKNLKLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNY-VNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCD--MQNVNDIINKHLIFINELSEIDSQI 5321
+ K +ER+ A + + D+ + WLD + + + T V D L+++K ++ Q G + K T ++ ++ I+ E L L+ W + I + L L+ G A++ + W+ + + GD +EV + + H+ +N++ H+ E +L + S +R+ +E + + + ++R + LQ +LQ A+ F+S++ + + L + E L S P E ++ H L Q+K + T + G+++L + + + + +W+ ++S+ E+R KL E+L ++E I L + + I P + V I +H +F NE++ QI
Sbjct: 6097 LNKTWKERLEKLEDAMQAAVQYQDTLQAMFDWLDNTVIKLCTMPPVGTDLNTVKDQLNEMKEFKVEVYQQQIEMEKLNHQGELMLKKAT-DETDRDIIREPLTELKHLWENLGEKIAHRQHKLEGALLALGQFQHALEELMSWLTHTEELLDAQRPISGDPKVIEVELAKHHVLKNDVLAHQATVETVNKAGNELLESSAGDDASSLRSRLEAMNQCWESVLQKTEEREQQLQSTLQQAQGFHSEIEDFLLELTRMESQLSA-SKPTGGLPETAREQLDTHMELYSQLKAKEETYNQLLDKGRLMLLSRDDSGSGSKTEQSVALLEQKWHVVSSKMEERKSKLEEALNLATEFQNSLQEFI----NWLTLAEQSLNIASPPSLILNTVLSQIEEHKVFANEVNAHRDQI 6499
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. UniProt/SwissProt
Match: sp|Q9QXZ0|MACF1_MOUSE (Microtubule-actin cross-linking factor 1 OS=Mus musculus OX=10090 GN=Macf1 PE=1 SV=2) HSP 1 Score: 243.432 bits (620), Expect = 9.466e-63 Identity = 187/690 (27.10%), Postives = 334/690 (48.41%), Query Frame = 3
Query: 4119 KIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIEN---FKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVE-DPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLKL--RMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFKLQNPSSFHKAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDL--------STNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEK----EQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPER---RSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYRF------GQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCR 6107
K+ +R ER R + A + K F +++ L WL+++ +++ + P K+ L K K K L + + T+R G LK+K + D +K L +L +R KW+ + V + L L+ +G DA++ + W+ + D + GDLD V L+D + + E+ + LK R L E + + ++ ++E+ ++ + L+++L+ A+EF ++ L WL +AE+ L+ + EA L LI+ H+ +K+V+E + + +G+ IL CHP + I +R+ ++ + ++ ++L +L L + L+EEL+ + + + IP ++ V +I +H F+ E++ + + K +K ++ H AP F + S+ SLN+ T + N R+N L+ ++ ++W L++E + L + L L E ++ FDF+ W+K ++ ++ K R+ D FRR + G +EF + I+ S FPT K + V +IF+++ + I Y +F+ +L P + R D DK I D + ++VA C C R+ V++ G+NKYRF G + +L +VR L STVMVR+GGGW + EFL KNDPCR
Sbjct: 6490 KVVQRSIERGRSLDDARKRAKQFHEAWKKLIDWLEDAESHLDSELEISNDPDKIKLQLSKHKEFQKTLGGKQPVYDTTIRTGRALKEKTLLAGDTQK--LDNLLGEVRDKWDTVCGKSVERQHKLEEALLFSGQFMDALQALVDWLYKVEPQLAEDQPVHGDLDLVMNLMDAHKVFQKELGKRTGTVQVLKRSGRELIEGSRDDTTWVKGQLQELSTRWDTVCKLSVSKQSRLEQALKQAEEFRDTVHMLLEWLSEAEQTLRFRGALPDDTEA-LQSLIDTHKEFMKKVEEKRVDVNTAVAMGEAILAVCHPDCITTIKHWITIIRARFEEVLTWAKQHQQRLETALSELVANAELLEELLAWIQWAETTLIQRDQEPIPQNIDRVKALITEHQSFMEEMTRKQPDVDRVTKTYKRKSVEPTH-AP---------FMEKSRSGSRKSLNQPTPPPMPILSQSEAKNPRINQLSARWQQVWLLALERQRKLNDALDRLEELCPELKEFANFDFDVWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILASKFPTTKLEMTAVADIFDRDGDGYIDYYEFVAALHPNKDAYRPTTDA-----DK------IEDEVTRQVAQCKCAKRFQVEQIGENKYRFFLGNQFGDSQQLRLVRILRSTVMVRVGGGWMALDEFLVKNDPCR 7155 HSP 2 Score: 60.4622 bits (145), Expect = 2.550e-7 Identity = 80/412 (19.42%), Postives = 172/412 (41.75%), Query Frame = 3
Query: 4122 IQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIENFKDQPT---KVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNL---KLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGK-VILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKI---QNEIRIEIPCD--MQNVNDIINKHLIFINELSEIDSQI 5321
+ K +ER+ A + + D+ + WLD + + + T V D L+++K ++ Q G + K T ++ ++ I+ E L L+ W + I + L L+ G A++ + W+ + + GD +EV + + H+ +N++ H+ + +L + S +R+ +E + + + ++R + LQ +LQ A+ F+S++ + + L + E L S P E ++ H L Q++ + + G+ ++L+ + + + + +W+ ++S+ E+R KL E+L +L E + E +N + + + I P + V I +H +F NE+++ QI
Sbjct: 6053 LNKTWKERLEKLEDAMQAAVQYQDTLQAMFDWLDNTVIKLCTMPPVGTDLNTVKDQLNEMKEFKVEVYQQQIEMEKLNHQGELMLKKAT-DETDRDIIREPLTELKHLWENLGEKIAHRQHKLEGALLALGQFQHALEELMSWLTHTEELLDAQRPISGDPKVIEVELAKHHVLKNDVLAHQATVATVNKAGSELLESSAGDDASSLRSRLETMNQCWESVLQKTEEREQQLQSTLQQAQGFHSEIEDFLLELNRMENQLSA-SKPTGGLPETAREQLDTHMELHSQLRAKEEIYNQLLDKGRLMLLSRGDSGSGSKTEQSVALLEQKWHAVSSKVEERKSKLEEAL-------SLATEFQNSLQEFINWLTLAEQSLNIASPPSLILNTVLSQIEEHKVFANEVNDHRDQI 6455
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. TrEMBL
Match: A0A2C9KRX1 (Uncharacterized protein OS=Biomphalaria glabrata OX=6526 GN=106066346 PE=4 SV=1) HSP 1 Score: 377.096 bits (967), Expect = 1.486e-103 Identity = 320/1207 (26.51%), Postives = 597/1207 (49.46%), Query Frame = 3
Query: 2601 IISQDLVKQQYSSLKKMGKDVTQFVEKLE--TDLVPKANQI----VKIGGQFCELEISEFTESLLEEIGITEDAIEEKDILFNKALYFVDFLSDEIKKVFEWFEVMENRLINFGEITPDQKLIEINLLELKSLNTEIFSKAQQLDKIGSAISEILSYPYVPNKNTLEEMKLNIKEKYSELVKGIIEKHNDLQKKYSDIGELDFSLDDNIGILQEFENQIKNLTPQSCKGDPEYTFSCFKNLKFLDDEFNGLQSDFQNLCERISAHGDIEDNTNLRCRGTLSIFGKLKEMKILTEQIRVLIPEKYVMIQEILKNEKLLSKEIHLHIDXXXXXXXXXXXXXXXGFSQESVEQSNLIFSNYIGDLNKQRNSIENSLKLASSHIESCQ-YDKCTFLSTAIKTLFHKYKRCQHLAAKRKEKLSIYSKLILEYDADKQTLQKDILELENLSIKRMN-PSKITVTCAEQIK---SIKISIQTADKNFDIYEHLTIRSFDIKCFGNRIDNSQLILELKEYGLKL--AKIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIENFK---DQPTKVLDHLDKLK------NLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLKLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFKLQNPSSFHKAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYRFGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRIANSLDLQKSKSMKMS 6155
+ +Q+L ++K+ ++ F ++LE +LV + QI + GQ +EI + E L D I ++ A D + + W E++L + +PD ++ + ELK ++I + ++ + + + ++ ++ +K+ + EK+SEL++G+ ++ N L +GE++ +++D I L E ++Q+ + + +GD +Y + + L+ ++ + L + + D+ +++ + + K EMK + +K +QE ++ K EI + + + + LK G +S E+ F L++Q IE L I+ C+ ++ +K L ++ + A KRKEK+ + L + + D + D L +S++ PSK+ QI + K + +N ++ + +K FG + D + ++ + G++L K+ +R ER R+ QAY+EDK F DS+ L WLD+S + F +QP +D+LK +QL+ ++ F T R G LKD+C DPE+ IL +ML+ L+ KWN +R + ++ L+ L+ +G + DA++ + W+ + A ++ +LGDLDTV +LI++ + E+ + L +R + + S++ + + + + R L+E+L+LA+EF + + +L +AE LK ++LP ++E + +LIEKHE + ++ + ++ + + IL++CHP A +V + T +RW+Q+ R+ R ++L E+L +++G+ AL+EEL+ + + + + R IP D++ V ++ +HL F E++ ++ + L KL S A +K Y ++ N D N R+ L ++ +W +S++ K LQ+ L E E + FDF+ W++ +L +I+ K R+TD FRRQ G+ EEF + ++KS FPTN+ L VF+IF+K +N I Y++F+D+L+ +R++++ KN N + K V L IH+ IEKE++ C C + + + KYRFG+ K +VR+LN TVMVR+GGGW T+ EFL++NDPCR + +++ +S
Sbjct: 16 MYNQELPGLDPEAIKEQQAELAGFKKELEKARELVGECRQIGHDLSNVCGQSGAIEIQKQMEDLSHMTDEVNDKIRDRGDELRGAFQHADHFKKLVDSINSWLPQAEHQLALMKQPSPDPNTLQRQIEELKVFKSDIHPHIVDMQQLVQEAASLKDISPLASEALMKPVKV-LNEKWSELIRGLTDRENQLTDMQLKVGEVNQAMEDIIVSLDEAQSQMDAM--EEVQGDTKYLETRIRKLELMEADIKNKDKVVHKLNKAVD---DMLQQSDVNVTES-PLHKKRIEMKEKLRNTSATLRQKENRLQESIRKIKRFLGEIDDQLAQVNEFRSELKTNEPFGALPDSAEKQYAEFVIKCQHLDEQERDIEQLLNTGQDLIDHCKSAHDVQDITDHVKRLKERWTDTKDRARKRKEKMEGHL-LNVSHFHDTLKVFTDWLNNAEISMRGCKYPSKLVKIVTNQIAEHNTFKADLLAQTENMNMLDQSGTY---LKFFGKKQDT--VYVKNMQVGIRLRWKKLLRRSDERTRLLEQAYKEDKRFDDSWHELCDWLDQSMQSMSKFALSANQPAGTKQDIDELKKHEMIVRFNQQLTLKHPTFFSTTRLGRNLKDRCVKSDPEREILNQMLEDLKNKWNSVRSVVSRSQNKLDEALLNSGRVSDALQSLLEWLQKAEAALDEEMPILGDLDTVHMLIEQHRNIQQELTAREATV--LTMRAPGNLPAQQASELAQLWDRVNHLCDI-------RESKLKEALKLAEEFQDVVTLMREFLPQAEAQLKFRALP--EDEMVIFQLIEKHEKFQEDLRNHQESVEKIKGLAEDILSSCHPNAVRFVKYYLTITQTRWDQLLHRATTRGQRLQEALRNIQGNAALLEELLAWLTDAQALLATKERDPIPDDLKVVETLLKEHLEFHEEVTSKNNDAERL---SKLVTSESKMAAQSKGYGSNMKL------NEFDGYN---------PRVIALQNKWRTVWHMSMDRKKRLQDAHDTLLELESFKNFDFDLWRQRYLNWIQAKKFRITDFFRRQDKDSDGFLDREEFISGMLKSKFPTNRTELSAVFDIFDKASNGFIEYKNFVDALKADRKNRI-KNNNRSAKSDVEL-IHEEIEKELSNCQCRQSFKAEWLEEGKYRFGEKQKTCLVRFLNHTVMVRVGGGWVTLEEFLEQNDPCRAKGRTNFDLRETLALS 1178
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. TrEMBL
Match: A0A210QRC3 (Dystonin OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT24659 PE=4 SV=1) HSP 1 Score: 378.252 bits (970), Expect = 1.656e-101 Identity = 252/811 (31.07%), Postives = 424/811 (52.28%), Query Frame = 3
Query: 3723 ESCQYDKCTFLSTAIKTLFHKYKRCQHLAAKRKEKLSIYSKLILEYDADKQTLQKDILELENLSIKRMN---PSKITVTCAEQIKSIKISIQTADKNFDIYEHLTIRSFDIKCFGNRIDNSQLILELKEYGLKLAKIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIENF-----KDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLKLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIK--DFKLQNPSSFHKAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYRFGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRIANSLD 6125
E C D T + ++ L ++ + A KRKEKLS + + + E+ TL+ L N I N PSK+ Q++ D++ + + L +K F + D + L L+ K+ +R ER R+ A+REDK F D++ L WLDES + F K TK +++D LK +L+ ++ F+ R G LKD+CT DPE+ +L +ML L+ KWN +R I ++ L+ L+ +G + DA+ + W+ + A D +LGDLDT+ +LI++ + E+ ++ +K N S +EE+E + R L++SLQLA+EF + + +L +AE LK ++LP +E +++LIEKHE + ++ + + + + IL NCHP A +V + T +RW+Q+ R+++R ++L E+L S++G+ AL+E+L+ + + + + R IP D++ V D+ H+ F +E++ ++ ++ L K + + ++P H + N+ N D N R+ L ++ +W++S++ K LQ+ L L E E + FDF WK +L +IK K +R+TD FRRQ R G + ++F N ++ + FPTN+ L VF+IF+++ + I Y+DFI++L+P R + N + N + +IHD +EKE++ C C + +R + KYRFG+ + +VR+LNSTVMVR+GGGW T+ EFL+ NDPCR +S D
Sbjct: 2512 EKCDPDDVTQICDRLRKLKDRWNDTKDRAQKRKEKLSEHLENVDEFHG---TLKTFTDWLNNAEIAMRNFKYPSKLVDRVTVQLEEHNKMKMELDQHSERMQTLDRTGSYLKHFCRKQDTIYIKNLLVGIRLRWKKLLRRTDERGRLLTHAHREDKRFYDAWKGLCDWLDESSKTVARFMSPVAKGSATK--ENIDDLKRFQHELAGKHTTFYSATRLGRNLKDRCTKSDPEREVLQQMLDDLKDKWNSVRSVISRSQNKLDEALLTSGRVTDALTSLLEWLGKAEATLAEDQPVLGDLDTIHMLIEQHKGMQQELGAREQTVAAMKA-----AGNVSAS----HLEELETTWDRVNRLSDHRENKLRDSLQLAEEFQDVVQVMREFLPQAEAELKYRALP--DDEVAIIQLIEKHEKFQEDLRNHQDCVDKIKLLAEEILQNCHPNAIRFVKYYLTITQTRWDQLLQRAKNRGQRLQEALRSIQGNAALVEDLLAWLTDAQVLLSTKERDPIPDDLKVVEDLYKDHVEFHDEITSKNNDVERLTKIINSEPKSPGRIHGS-------------NMKLNENDIFN---------PRVITLQNKWRIVWRMSVDRKKNLQDALDTLLELESFKTFDFGVWKAKYLNWIKAKKMRVTDFFRRQDKRGDGLLLRDDFVNGMLITRFPTNRTELHAVFDIFDRDGRDVIEYKDFIEALKPTRHQKTRLNSS-NRQMTDAELIHDNVEKEISGCQCRNSFRAERLSEGKYRFGEKQNIRLVRFLNSTVMVRVGGGWVTLDEFLETNDPCRAGSSGD 3283
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. TrEMBL
Match: A0A0L8H184 (Uncharacterized protein OS=Octopus bimaculoides OX=37653 GN=OCBIM_22024678mg PE=4 SV=1) HSP 1 Score: 348.591 bits (893), Expect = 7.988e-93 Identity = 293/1095 (26.76%), Postives = 540/1095 (49.32%), Query Frame = 3
Query: 2865 EIKKVFEWFEVMENRLINFGEITPDQKLIEINLLELKSLNTEIFSKAQQLDKIGSAISEILSYPYVPNKNTLEEMKLNIKEKYSELVKGIIEKHNDLQKKYSDIGELDFSLDDNIGILQEFENQIKNLTPQSCKGDPEYTFSCFKNLKFLDDEFNG---LQSDFQNLCER-ISAHGDIEDNTNLRCRGTLSIFGKLKEMKILTEQIRVLIPEKYVMIQEILKNEKLLSKEIHLHIDXXXXXXXXXXXXXXXGFSQESVEQSNLIFSNYIGDLNKQRNSIENSLKLASSHIESCQYDKCTFLSTAIKTLFHKYKRCQHLAAKRKEKLSIYSKLILEYDADKQTLQKDILELENLSIKRMNPSKITVTCAEQIKSIKISIQTADKNFDIYEHLTIRSFDIKCFGNRIDNSQLILELKEYGLKLAKIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIENFKD---------QPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLKLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFKLQNPSSFHKAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVV-TLIIHDIIEKEVAVCTCTLRYYVKREGDNKYRFGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCR 6107
E+ + W V +L + D+ + + ELK + +D + ++ + V + ++ + +I E++++L+KGI ++ ++L + +GE+D ++++ +G L + I +L GD + + K L+ + D+ S +N + IS GD + R T + L+E++I T+ ++ +QE L+ K S ++ + ++ L LK P G E+ F +L+ + + +++ ++ CQ + LS +K L ++ ++ +R++K+ + + + E+ + + E L PSKI EQIK + + + L +K FG + D + L L+ K+ +R ER R+ Q+Y ED+ F +S+ +L WLD S M+ +F Q K+ + +D+LK QL+ +++ ++ T R G LKD+CT D ++ IL ML L+ KWN +R + ++ L+ L+ +G + DA+ + W++ + A D +LGD DTV +LI++ + E+ ++ + LK + E I + + + + +L + R L+E+L LA EF + + +L E LK + LP +E ++ LIE+HE ++++ + T+ + + IL +CHP A +V + T +RW+Q+ RS R+++L ++L S++G+ AL+EEL+ + +C + + + IP D++ V ++ +H E++ ++ L K +S K R+ Y + WR LN+ N R+ L ++ +W++S++ K LQ+ L L E E + FDF+ W++ +L +I+ K LR+TD FRRQ G +EF +I++ FPTNK L VF+IF+ N+ +I Y++FI +L+P+R Q + ++ + +IHD +E++++ C C ++ +R D +YRFG+N KL +VR+LNS VMVR+GGGW T+ EFL+ NDPCR
Sbjct: 848 ELLAIRSWLPVSRQKLSVMKVPSTDKAALRHQIEELKLFKMQTHPHVADVDTLNQHMTALNDLSPVAAEYLMKPVS-DINEQWNDLIKGIADRESNLNETQVKVGEIDNAIENVLGSLTGIKTDIDSL--DEVIGDSKCIETQLKKLQLIQDDVKNQSLTTSKLKNAVDNLISRSGDADSPLQ---RKTNEVSKLLREVEIKTK-------DQENKLQETLRYVKKFSGDVDDMLHWITDLRMELKSQPPFGALPETARGQLDNFIKRCEELDIKEDCVKSLQINGQDMMDRCQPENIFQLSEKLKKLRERWHETKNRTLRRRDKMKEHLQHVEEFHTTLKKFSDWLNSAETLMRGFKFPSKIVEKVCEQIKEHADFKKDVQSYTEWMQKLDRTGTYLKYFGRKQDTIYIKNLLVGIRLRWKKLLRRTDERERLLQQSYHEDRRFEESWKDLCDWLDSSEDMLTSFLTPQVQASPLTQVNKMKEEMDELKLFQHQLAAKHSLYYSTTRLGRNLKDRCTKSDQDRDILQAMLDQLKNKWNLVRSVVSKSQNKLDEALLTSGRVSDALNSLQEWLSKAEAMLSKDECILGDYDTVNLLIEQHKSVQQELEAREQTLEALK-----SAGSIPEQHIEDLVF-LWKRINLLS---ESREARLKEALTLADEFQEVVQVMREFLPTVESELKFRPLP--DDELSIIHLIERHEKFQERLRNHQDTVDKIKVLAERILKHCHPNAVRFVKYYLTITQTRWDQLIQRSHSRAQRLQDALKSIQGNAALLEELLAWLTDCQVLLATKEKDPIPEDIRVVETLLKEHQDLHEEITSKNADADRLTKIV-----TSESKLSQGRH------YGSNWR-----LNEID---GHNPRVIALQNKWRTVWRMSVDRKKKLQDALETLMELESFKNFDFDLWRQRYLGWIQEKKLRITDFFRRQDKDCDGALTRKEFVEGMIQTKFPTNKTELNAVFDIFDPNHTGKIVYRNFIAALKPDRHKQA--RLKTGKRQATDSEMIHDEVERQISQCQCRHQFKAERIQDGQYRFGENLKLKLVRFLNSCVMVRVGGGWVTLDEFLETNDPCR 1897
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. TrEMBL
Match: A0A0L8H273 (Uncharacterized protein OS=Octopus bimaculoides OX=37653 GN=OCBIM_22024678mg PE=4 SV=1) HSP 1 Score: 343.584 bits (880), Expect = 2.461e-91 Identity = 294/1098 (26.78%), Postives = 540/1098 (49.18%), Query Frame = 3
Query: 2865 EIKKVFEWFEVMENRLINFGEITPDQKLIEINLLELKSLNTEIFSKAQQLDKIGSAISEILSYPYVPNKNTLEEMKLNIKEKYSELVKGII---EKHNDLQKKYSDIGELDFSLDDNIGILQEFENQIKNLTPQSCKGDPEYTFSCFKNLKFLDDEFNG---LQSDFQNLCER-ISAHGDIEDNTNLRCRGTLSIFGKLKEMKILTEQIRVLIPEKYVMIQEILKNEKLLSKEIHLHIDXXXXXXXXXXXXXXXGFSQESVEQSNLIFSNYIGDLNKQRNSIENSLKLASSHIESCQYDKCTFLSTAIKTLFHKYKRCQHLAAKRKEKLSIYSKLILEYDADKQTLQKDILELENLSIKRMNPSKITVTCAEQIKSIKISIQTADKNFDIYEHLTIRSFDIKCFGNRIDNSQLILELKEYGLKLAKIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIENFKD---------QPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLKLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFKLQNPSSFHKAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVV-TLIIHDIIEKEVAVCTCTLRYYVKREGDNKYRFGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCR 6107
E+ + W V +L + D+ + + ELK + +D + ++ + V + ++ + +I E++++L+KGI E+ ++L + +GE+D ++++ +G L + I +L GD + + K L+ + D+ S +N + IS GD + R T + L+E++I T+ ++ +QE L+ K S ++ + ++ L LK P G E+ F +L+ + + +++ ++ CQ + LS +K L ++ ++ +R++K+ + + + E+ + + E L PSKI EQIK + + + L +K FG + D + L L+ K+ +R ER R+ Q+Y ED+ F +S+ +L WLD S M+ +F Q K+ + +D+LK QL+ +++ ++ T R G LKD+CT D ++ IL ML L+ KWN +R + ++ L+ L+ +G + DA+ + W++ + A D +LGD DTV +LI++ + E+ ++ + LK + E I + + + + +L + R L+E+L LA EF + + +L E LK + LP +E ++ LIE+HE ++++ + T+ + + IL +CHP A +V + T +RW+Q+ RS R+++L ++L S++G+ AL+EEL+ + +C + + + IP D++ V ++ +H E++ ++ L K +S K R+ Y + WR LN+ N R+ L ++ +W++S++ K LQ+ L L E E + FDF+ W++ +L +I+ K LR+TD FRRQ G +EF +I++ FPTNK L VF+IF+ N+ +I Y++FI +L+P+R Q + ++ + +IHD +E++++ C C ++ +R D +YRFG+N KL +VR+LNS VMVR+GGGW T+ EFL+ NDPCR
Sbjct: 848 ELLAIRSWLPVSRQKLSVMKVPSTDKAALRHQIEELKLFKMQTHPHVADVDTLNQHMTALNDLSPVAAEYLMKPVS-DINEQWNDLIKGIADREEEQSNLNETQVKVGEIDNAIENVLGSLTGIKTDIDSL--DEVIGDSKCIETQLKKLQLIQDDVKNQSLTTSKLKNAVDNLISRSGDADSPLQ---RKTNEVSKLLREVEIKTK-------DQENKLQETLRYVKKFSGDVDDMLHWITDLRMELKSQPPFGALPETARGQLDNFIKRCEELDIKEDCVKSLQINGQDMMDRCQPENIFQLSEKLKKLRERWHETKNRTLRRRDKMKEHLQHVEEFHTTLKKFSDWLNSAETLMRGFKFPSKIVEKVCEQIKEHADFKKDVQSYTEWMQKLDRTGTYLKYFGRKQDTIYIKNLLVGIRLRWKKLLRRTDERERLLQQSYHEDRRFEESWKDLCDWLDSSEDMLTSFLTPQVQASPLTQVNKMKEEMDELKLFQHQLAAKHSLYYSTTRLGRNLKDRCTKSDQDRDILQAMLDQLKNKWNLVRSVVSKSQNKLDEALLTSGRVSDALNSLQEWLSKAEAMLSKDECILGDYDTVNLLIEQHKSVQQELEAREQTLEALK-----SAGSIPEQHIEDLVF-LWKRINLLS---ESREARLKEALTLADEFQEVVQVMREFLPTVESELKFRPLP--DDELSIIHLIERHEKFQERLRNHQDTVDKIKVLAERILKHCHPNAVRFVKYYLTITQTRWDQLIQRSHSRAQRLQDALKSIQGNAALLEELLAWLTDCQVLLATKEKDPIPEDIRVVETLLKEHQDLHEEITSKNADADRLTKIV-----TSESKLSQGRH------YGSNWR-----LNEID---GHNPRVIALQNKWRTVWRMSVDRKKKLQDALETLMELESFKNFDFDLWRQRYLGWIQEKKLRITDFFRRQDKDCDGALTRKEFVEGMIQTKFPTNKTELNAVFDIFDPNHTGKIVYRNFIAALKPDRHKQA--RLKTGKRQATDSEMIHDEVERQISQCQCRHQFKAERIQDGQYRFGENLKLKLVRFLNSCVMVRVGGGWVTLDEFLETNDPCR 1900
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. TrEMBL
Match: A0A0L8H0R3 (Uncharacterized protein OS=Octopus bimaculoides OX=37653 GN=OCBIM_22024678mg PE=4 SV=1) HSP 1 Score: 338.576 bits (867), Expect = 9.646e-90 Identity = 293/1112 (26.35%), Postives = 540/1112 (48.56%), Query Frame = 3
Query: 2865 EIKKVFEWFEVMENRLINFGEITPDQKLIEINLLELKSLNTEIFSKAQQLDKIGSAISEILSYPYVPNKNTLEEMKLNIKEKYSELVKGIIEKHNDLQKKYSDIGELDFSLDDNIGILQEFENQIKNLTPQSCKGDPEYTFSCFKNLKFLDDEFNG---LQSDFQNLCER-ISAHGDIEDNTNLRCRGTLSIFGKLKEMKILTEQIRVLIPEKYVMIQEILKNEKLLSKEIHLHIDXXXXXXXXXXXXXXXGFSQESVEQSNLIFSNYIGDLNKQRNSIENSLKLASSHIESCQYDKCTFLSTAIKTLFHKYKRCQHLAAKRKEKLSIYSKLILEYDADKQTLQKDILELENLSIKRMNPSKITVTCAEQIKSIKISIQTADKNFDIYEHLTIRSFDIKCFGNRIDNSQLILELKEYGLKLAKIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIENFKD---------QPTKVLDHLDKLKN-----------------LCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLKLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFKLQNPSSFHKAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVV-TLIIHDIIEKEVAVCTCTLRYYVKREGDNKYRFGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCR 6107
E+ + W V +L + D+ + + ELK + +D + ++ + V + ++ + +I E++++L+KGI ++ ++L + +GE+D ++++ +G L + I +L GD + + K L+ + D+ S +N + IS GD + R T + L+E++I T+ ++ +QE L+ K S ++ + ++ L LK P G E+ F +L+ + + +++ ++ CQ + LS +K L ++ ++ +R++K+ + + + E+ + + E L PSKI EQIK + + + L +K FG + D + L L+ K+ +R ER R+ Q+Y ED+ F +S+ +L WLD S M+ +F Q K+ + +D+LK QL+ +++ ++ T R G LKD+CT D ++ IL ML L+ KWN +R + ++ L+ L+ +G + DA+ + W++ + A D +LGD DTV +LI++ + E+ ++ + LK + E I + + + + +L + R L+E+L LA EF + + +L E LK + LP +E ++ LIE+HE ++++ + T+ + + IL +CHP A +V + T +RW+Q+ RS R+++L ++L S++G+ AL+EEL+ + +C + + + IP D++ V ++ +H E++ ++ L K +S K R+ Y + WR LN+ N R+ L ++ +W++S++ K LQ+ L L E E + FDF+ W++ +L +I+ K LR+TD FRRQ G +EF +I++ FPTNK L VF+IF+ N+ +I Y++FI +L+P+R Q + ++ + +IHD +E++++ C C ++ +R D +YRFG+N KL +VR+LNS VMVR+GGGW T+ EFL+ NDPCR
Sbjct: 848 ELLAIRSWLPVSRQKLSVMKVPSTDKAALRHQIEELKLFKMQTHPHVADVDTLNQHMTALNDLSPVAAEYLMKPVS-DINEQWNDLIKGIADRESNLNETQVKVGEIDNAIENVLGSLTGIKTDIDSL--DEVIGDSKCIETQLKKLQLIQDDVKNQSLTTSKLKNAVDNLISRSGDADSPLQ---RKTNEVSKLLREVEIKTK-------DQENKLQETLRYVKKFSGDVDDMLHWITDLRMELKSQPPFGALPETARGQLDNFIKRCEELDIKEDCVKSLQINGQDMMDRCQPENIFQLSEKLKKLRERWHETKNRTLRRRDKMKEHLQHVEEFHTTLKKFSDWLNSAETLMRGFKFPSKIVEKVCEQIKEHADFKKDVQSYTEWMQKLDRTGTYLKYFGRKQDTIYIKNLLVGIRLRWKKLLRRTDERERLLQQSYHEDRRFEESWKDLCDWLDSSEDMLTSFLTPQVQASPLTQVNKMKEEMDELKPKPVDMETRISQLLHVKLFQHQLAAKHSLYYSTTRLGRNLKDRCTKSDQDRDILQAMLDQLKNKWNLVRSVVSKSQNKLDEALLTSGRVSDALNSLQEWLSKAEAMLSKDECILGDYDTVNLLIEQHKSVQQELEAREQTLEALK-----SAGSIPEQHIEDLVF-LWKRINLLS---ESREARLKEALTLADEFQEVVQVMREFLPTVESELKFRPLP--DDELSIIHLIERHEKFQERLRNHQDTVDKIKVLAERILKHCHPNAVRFVKYYLTITQTRWDQLIQRSHSRAQRLQDALKSIQGNAALLEELLAWLTDCQVLLATKEKDPIPEDIRVVETLLKEHQDLHEEITSKNADADRLTKIV-----TSESKLSQGRH------YGSNWR-----LNEID---GHNPRVIALQNKWRTVWRMSVDRKKKLQDALETLMELESFKNFDFDLWRQRYLGWIQEKKLRITDFFRRQDKDCDGALTRKEFVEGMIQTKFPTNKTELNAVFDIFDPNHTGKIVYRNFIAALKPDRHKQA--RLKTGKRQATDSEMIHDEVERQISQCQCRHQFKAERIQDGQYRFGENLKLKLVRFLNSCVMVRVGGGWVTLDEFLETNDPCR 1914
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Cavefish
Match: DST (dystonin [Source:HGNC Symbol;Acc:HGNC:1090]) HSP 1 Score: 238.81 bits (608), Expect = 1.506e-62 Identity = 184/681 (27.02%), Postives = 342/681 (50.22%), Query Frame = 3
Query: 4119 KIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIEN---FKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLK--LRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKI-KSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFK---LQNPSSFHKAPT---KRYHDRVEFYKNIWRNSKDSLNKSTDDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYR------FGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCR 6107
K+ +R ER R+ + A + K F +S+ L WL+ES +++ + P K+ L + K K L +++ + T R G LKDK +++D + L +ML LR KW+ + V ++ L L+ +G DA++ + W+ + D + GD+D V LID + + E+ + LK R L E + + S ++ ++E+ ++ + L+++L A+EF+S ++ L WL +AE+ L+ SLP +E L LIE+H+ +K+++E + + ++G+ IL CHP + V +R+ ++ + + ++L+ + L + L+E L+ + + + + +P +++ V +I++H F+ E++ + + K K P + P R + +N++ + + + N R+N L +++ ++W L+++ + L + L L E ++ FDF+ W+K ++ ++ K R+ D FRR + G +EF I+ S FPT++ + V +IF+++ + I Y +F+ +L P + + K + DK I D + ++VA C C R+ V++ G NKYR FG + +L +VR L STVMVR+GGGW + EFL KNDPCR
Sbjct: 920 KVVQRSVERGRLLDDARKRAKQFHESWTKLMEWLEESERALDSELEIANDPDKIKLQLAQHKEFQKALGGKHSVYDTTGRTGRALKDKTSLKD-DNQKLDDMLCELRDKWDTVCGKSVERQNKLEEALLFSGQFTDALQALIDWLYKVEPQLAEDQPVHGDIDLVLNLIDSHKVFQKELGKRTGSVQALKRSARDLVENTHDDSSWVKVQMQELSTRWETVCALSVSKQTRLEQALCQAEEFHSTVHILLEWLAEAEQSLRFHGSLP--DDEEALRSLIEQHKEFMKKLEEKRVALGKAISMGEAILAICHPDSITTVKHWNTIIKARFEEVTAWARQHDQRLSTAFTELLATQELLESLLSWLQWAETTLTEKDKEPLPQEIEEVKALISEHQTFMEEMTRKQPDVDKVTKTHKRKATAEPLVQSQIPVLEKGRTCRKRSPTQNMYPATAQPQIE-----TKNPRVNLLVSKWQQVWLLALDRRRKLNDALDRLEELKEFANFDFDVWRKRYMRWMNHKKSRVMDFFRRIDKDQDGKVTRQEFIEGILSSKFPTSRLEMSAVADIFDRDGDGYIDYYEFVAALHPNKDAY--KPLTDADK------IEDEVTRQVAKCKCPKRFQVEQIGANKYRFYLGNQFGDSQQLRLVRILRSTVMVRVGGGWMALDEFLVKNDPCR 1584
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Cavefish
Match: MACF1 (microtubule actin crosslinking factor 1 [Source:HGNC Symbol;Acc:HGNC:13664]) HSP 1 Score: 239.195 bits (609), Expect = 1.998e-62 Identity = 180/680 (26.47%), Postives = 337/680 (49.56%), Query Frame = 3
Query: 4119 KIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIEN---FKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTV-EDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLKLRMLNEIKNYEESD---IRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFKLQNPSSFHKAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPER---RSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYR------FGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRI 6110
K+ +R +R R ++A + K F +++ L WL+E+ +++ ++P K+ L K K K L + + T+R G ++DK T+ D EK L +L +R KW+ + V + L L+ +G +A++ + W+ + D + GDLD V L+D + E+ + LK R E+ + D ++ ++E+ N++ + LQ++L+ A+EF + + L WL +AE+ L+ + + ++E L EL+ H ++ V+E + + +G+ IL CHP + I +R+ ++ + ++ ++L +L L + AL+EEL+ + + +P D+ + ++++H +F+ E++ + + K +K + P+ + +R R + + S+ + N RL L +++ ++W L+++ + L + L L E ++ FDF+ W+K ++ ++ K R+ D FRR + G +EF + I+ S FPT+K + V +IF+++ + I Y +F+ +L P + R D DK I D + ++VA C C R+ V++ G+NKYR FG + +L +VR L STVMVR+GGGW + EFL KNDPCR+
Sbjct: 4476 KLVQRSLDRGRHLDEARKRAKQFHEAWRKLTDWLEEAESRLDSELEISNEPDKIKVQLTKHKEFQKALGSKQPVYDTTVRSGRAMRDKATLPADTEK--LDHLLGEVRDKWDTVCGKSVERQHKLEEALLFSGQFAEALQALVDWLYRVEPQLAEDQPVHGDLDLVSNLMDSHKAFQKELGKRTSSVQALK-RSARELMDTGRDDTAWVKVQLQELSNRWDTVCTLSVSKQTRLQQALKQAEEFRTAVQMLLEWLSEAEQTLRFRGVLPEESET-LQELLHTHRDFMQTVEEKRVDVNKAAGMGEGILAVCHPDCITTIKHWITIIRARFEEVLTWAKQHEQRLETALTELLNNAALLEELLSWLQWAETTLVQRDTEPLPQDIPQLKTLMSEHQMFMEEMTRKQPDVDKVTKTYK-RKPAEPPSSLAERRGTRKQ------QQSQQQQQHAVQVTGGNPRLTQLCSRWQQVWLLALDRQRKLHDALDRLEELKEFANFDFDVWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILASKFPTSKLEMMAVADIFDRDGDGYIDYYEFVAALHPNKDAYRPTTDA-----DK------IEDEVTRQVAQCKCAKRFQVEQIGENKYRFFLGNQFGDSQQLRLVRILRSTVMVRVGGGWMALDEFLVKNDPCRV 5133 HSP 2 Score: 75.8702 bits (185), Expect = 5.954e-13 Identity = 82/418 (19.62%), Postives = 177/418 (42.34%), Query Frame = 3
Query: 4122 IQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMI---ENFKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHK---EDFKNLKLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVIL-----TNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCD--MQNVNDIINKHLIFINELSEIDSQIKSLIK 5336
+ + RER+ +A + D+ + +LD + + + + + V +++LK ++ Q + G L K T + ++ ++ E L LR W+ + I + L L+ G A+ + W++ + A + D +E+ + + H+ N++ +H+ E + +L+ E S +R+ ++E+ + + ++R K L+ +LQ A+ F+ +L E WLR+ E L + P E +++H L Q+ + + G+ +L P T L ++W +N++ +DR KL E++ G + +++ I L + + + + P + V I++H +F+NE++ Q+ +L K
Sbjct: 4036 LNRTWRERMEKLEEAMTASVQYQDALQGMFDYLDNAVIKLCEMQTIGTDLSTVKQQIEELKQYKVEVYQQQIDMEKLCHQGELLLKKVT-DQTDRDMIQEPLTELRHLWDNLGEKITLRQHKLEGALLALGQFQHALSELQSWLSHTHATLDTQRPVSSDPKAIEIELAKHHVLRNDVLSHRATVETVNSAGSELLDSSPGDEASHLRDQLDELNRSWDSLLLKTKERQKLLEAALQQAEGFHGELEEFLQWLRRTESQLS-AAKPTGGLPETAREQLQQHMELQSQLSQRGEQYHRLLDQGESMLLARGGEEAGPGTTQTQQNLAL-LQNKWASLNTKMDDRRAKLEEAVSLATGFQSSLQDTI----NWLTQAEQTLNMAQPPSLILDTVLFQIDEHKVFVNEVNTHREQVLALEK 4446 HSP 3 Score: 61.6178 bits (148), Expect = 1.318e-8 Identity = 108/522 (20.69%), Postives = 211/522 (40.42%), Query Frame = 3
Query: 4074 SQLILELKEYGLKLAKIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIENFKDQ---PTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVED--PEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNL-----KLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSLPKFQNEADLVEL-IEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCH-PMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESL---GSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFKLQNPSSFHKAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRF 5594
S L +L E + + +ER ++ A ++ + F WL + + K P + L + L QLS + +H L G + E+ P + L L+ KW + + ++ L + +++ W+ + LDTV IDE + NE+N H+E L +LR + ++ I+N + ++ ++ V R + L E+ + AK+F+ +L WL +AE ++ S + NE D +++ + KH+ K + + G+ + P T ++ L+ + +W+ + +S +R KL E+L G + + + +Y+V L + Q + D+ V+++++ H F EL + S +++L + + + + T +++ N W D++ T +S TRL + K + E + A+Q L L+E EQ RF
Sbjct: 4239 SHLRDQLDELNRSWDSLLLKTKERQKLLEAALQQAEGFHGELEEFLQWLRRTESQLSAAKPTGGLPETAREQLQQHMELQSQLSQRGEQYHRLLDQGESMLLARGGEEAGPGTTQTQQNLALLQNKWASLNTKMDDRRAKLEEAVSLATGFQSSLQDTINWLTQAEQTLNMAQPPSLILDTVLFQIDEHKVFVNEVNTHREQVLALEKAGSQLRFASLKQDV--VLIKNLLLSVQARWDKLVQRSLDRGRHLDEARKRAKQFHEAWRKLTDWLEEAES--RLDSELEISNEPDKIKVQLTKHKEFQKALGSKQPVYDTTVRSGRAMRDKATLPADTEKLDHLLGEVRDKWDTVCGKSVERQHKLEEALLFSGQFAEALQALVDWLYRVEPQLAEDQ-----PVHGDLDLVSNLMDSHKAFQKELGKRTSSVQALKRSARELMDTG--RDDTAWVKVQLQELSNRW----DTV--CTLSVSKQTRL-------QQALKQAEEFRTAVQMLLEWLSEAEQTLRF 4736
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Cavefish
Match: MACF1 (microtubule actin crosslinking factor 1 [Source:HGNC Symbol;Acc:HGNC:13664]) HSP 1 Score: 238.424 bits (607), Expect = 3.780e-62 Identity = 179/684 (26.17%), Postives = 339/684 (49.56%), Query Frame = 3
Query: 4119 KIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIEN---FKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTV-EDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLKLRMLNEIKNYEESD---IRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFKLQNPSSFHKAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYR------FGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRIANSLDLQ 6131
K+ +R +R R ++A + K F +++ L WL+E+ +++ ++P K+ L K K K L + + T+R G ++DK T+ D EK L +L +R KW+ + V + L L+ +G +A++ + W+ + D + GDLD V L+D + E+ + LK R E+ + D ++ ++E+ N++ + LQ++L+ A+EF + + L WL +AE+ L+ + + ++E L EL+ H ++ V+E + + +G+ IL CHP + I +R+ ++ + ++ ++L +L L + AL+EEL+ + + +P D+ + ++++H +F+ E++ + + K +K + P+ + +R R + + S+ + N RL L +++ ++W L+++ + L + L L E ++ FDF+ W+K ++ ++ K R+ D FRR + G +EF + I+ S FPT+K + V +IF+++ + I Y +F+ +L P + + + DK I D + ++VA C C R+ V++ G+NKYR FG + +L +VR L STVMVR+GGGW + EFL KNDPCR +L+
Sbjct: 4824 KLVQRSLDRGRHLDEARKRAKQFHEAWRKLTDWLEEAESRLDSELEISNEPDKIKVQLTKHKEFQKALGSKQPVYDTTVRSGRAMRDKATLPADTEK--LDHLLGEVRDKWDTVCGKSVERQHKLEEALLFSGQFAEALQALVDWLYRVEPQLAEDQPVHGDLDLVSNLMDSHKAFQKELGKRTSSVQALK-RSARELMDTGRDDTAWVKVQLQELSNRWDTVCTLSVSKQTRLQQALKQAEEFRTAVQMLLEWLSEAEQTLRFRGVLPEESET-LQELLHTHRDFMQTVEEKRVDVNKAAGMGEGILAVCHPDCITTIKHWITIIRARFEEVLTWAKQHEQRLETALTELLNNAALLEELLSWLQWAETTLVQRDTEPLPQDIPQLKTLMSEHQMFMEEMTRKQPDVDKVTKTYK-RKPAEPPSSLAERRGTRKQ------QQSQQQQQHAVQVTGGNPRLTQLCSRWQQVWLLALDRQRKLHDALDRLEELKEFANFDFDVWRKKYMRWMNHKKSRVMDFFRRIDKDQDGKITRQEFIDGILASKFPTSKLEMMAVADIFDRDGDGYIDYYEFVAALHPNKDAY--RPTTDADK------IEDEVTRQVAQCKCAKRFQVEQIGENKYRFFLGNQFGDSQQLRLVRILRSTVMVRVGGGWMALDEFLVKNDPCRARGRTNLE 5488 HSP 2 Score: 75.8702 bits (185), Expect = 7.370e-13 Identity = 82/418 (19.62%), Postives = 177/418 (42.34%), Query Frame = 3
Query: 4122 IQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMI---ENFKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHK---EDFKNLKLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVIL-----TNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCD--MQNVNDIINKHLIFINELSEIDSQIKSLIK 5336
+ + RER+ +A + D+ + +LD + + + + + V +++LK ++ Q + G L K T + ++ ++ E L LR W+ + I + L L+ G A+ + W++ + A + D +E+ + + H+ N++ +H+ E + +L+ E S +R+ ++E+ + + ++R K L+ +LQ A+ F+ +L E WLR+ E L + P E +++H L Q+ + + G+ +L P T L ++W +N++ +DR KL E++ G + +++ I L + + + + P + V I++H +F+NE++ Q+ +L K
Sbjct: 4384 LNRTWRERMEKLEEAMTASVQYQDALQGMFDYLDNAVIKLCEMQTIGTDLSTVKQQIEELKQYKVEVYQQQIDMEKLCHQGELLLKKVT-DQTDRDMIQEPLTELRHLWDNLGEKITLRQHKLEGALLALGQFQHALSELQSWLSHTHATLDTQRPVSSDPKAIEIELAKHHVLRNDVLSHRATVETVNSAGSELLDSSPGDEASHLRDQLDELNRSWDSLLLKTKERQKLLEAALQQAEGFHGELEEFLQWLRRTESQLS-AAKPTGGLPETAREQLQQHMELQSQLSQRGEQYHRLLDQGESMLLARGGEEAGPGTTQTQQNLAL-LQNKWASLNTKMDDRRAKLEEAVSLATGFQSSLQDTI----NWLTQAEQTLNMAQPPSLILDTVLFQIDEHKVFVNEVNTHREQVLALEK 4794 HSP 3 Score: 61.2326 bits (147), Expect = 1.657e-8 Identity = 108/522 (20.69%), Postives = 211/522 (40.42%), Query Frame = 3
Query: 4074 SQLILELKEYGLKLAKIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIENFKDQ---PTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVED--PEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNL-----KLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSLPKFQNEADLVEL-IEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCH-PMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESL---GSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFKLQNPSSFHKAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRF 5594
S L +L E + + +ER ++ A ++ + F WL + + K P + L + L QLS + +H L G + E+ P + L L+ KW + + ++ L + +++ W+ + LDTV IDE + NE+N H+E L +LR + ++ I+N + ++ ++ V R + L E+ + AK+F+ +L WL +AE ++ S + NE D +++ + KH+ K + + G+ + P T ++ L+ + +W+ + +S +R KL E+L G + + + +Y+V L + Q + D+ V+++++ H F EL + S +++L + + + + T +++ N W D++ T +S TRL + K + E + A+Q L L+E EQ RF
Sbjct: 4587 SHLRDQLDELNRSWDSLLLKTKERQKLLEAALQQAEGFHGELEEFLQWLRRTESQLSAAKPTGGLPETAREQLQQHMELQSQLSQRGEQYHRLLDQGESMLLARGGEEAGPGTTQTQQNLALLQNKWASLNTKMDDRRAKLEEAVSLATGFQSSLQDTINWLTQAEQTLNMAQPPSLILDTVLFQIDEHKVFVNEVNTHREQVLALEKAGSQLRFASLKQDV--VLIKNLLLSVQARWDKLVQRSLDRGRHLDEARKRAKQFHEAWRKLTDWLEEAES--RLDSELEISNEPDKIKVQLTKHKEFQKALGSKQPVYDTTVRSGRAMRDKATLPADTEKLDHLLGEVRDKWDTVCGKSVERQHKLEEALLFSGQFAEALQALVDWLYRVEPQLAEDQ-----PVHGDLDLVSNLMDSHKAFQKELGKRTSSVQALKRSARELMDTG--RDDTAWVKVQLQELSNRW----DTV--CTLSVSKQTRL-------QQALKQAEEFRTAVQMLLEWLSEAEQTLRF 5084
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Cavefish
Match: macf1b (microtubule actin crosslinking factor 1b [Source:ZFIN;Acc:ZDB-GENE-100922-36]) HSP 1 Score: 115.931 bits (289), Expect = 3.231e-28 Identity = 67/180 (37.22%), Postives = 102/180 (56.67%), Query Frame = 3
Query: 5577 EQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPER---RSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYRFGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCR 6107
E+ FDFN W+K ++ +I R+ D+FR G +EF + ++ S FPTN + V NIF+ N++ I Y +F+ +L P R R +D + I++ + ++V+ C C R+ V++ N+YRFG + +L MVR L ST+MVR+GGGW + EFL KNDPCR
Sbjct: 4 EEFANFDFNLWRKRYMQWISHLKSRILDVFRAIDRDHDGRITQKEFVDSVLAS-FPTNSLEMTAVANIFDVNSDGYIDYYEFVSALHPSRDPYRRTIDADQ-----------INEEVSRQVSQCNCPKRFQVEQISANRYRFGDSQQLRMVRILRSTLMVRVGGGWTALDEFLVKNDPCR 171
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Cavefish
Match: ENSAMXT00000038065.1 (pep primary_assembly:Astyanax_mexicanus-2.0:3:11675492:11682368:1 gene:ENSAMXG00000041735.1 transcript:ENSAMXT00000038065.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 70.0922 bits (170), Expect = 2.147e-11 Identity = 30/80 (37.50%), Postives = 50/80 (62.50%), Query Frame = 3
Query: 5880 DKKVVTLIIHDI-IEKEVAVCTCTLRYYVKREGDNKYRFGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRIAN 6116
+K+ V L + + ++ ++ CTC ++ + + + KYR G +S L VR L + VMVR+GGGW T+ +L K+DPCR +
Sbjct: 5 NKRCVCLYLSCVQVQHLISRCTCPTQFPMVKISEGKYRVGDSSTLIFVRILRNHVMVRVGGGWDTLEHYLDKHDPCRCTS 84
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Sea Lamprey
Match: MACF1 (microtubule actin crosslinking factor 1 [Source:HGNC Symbol;Acc:HGNC:13664]) HSP 1 Score: 236.498 bits (602), Expect = 2.540e-62 Identity = 202/807 (25.03%), Postives = 388/807 (48.08%), Query Frame = 3
Query: 3753 LSTAIKTLFHKYKRCQHLAAKRKEKLSIYSKLILEYDADKQTLQKDILELENLSIKRMNPSKITV-TCAEQIKSIKISIQTADKNFDIYEHLTIRSFDIKCFGNRIDNSQLILELKEYGLKLAKIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIENFKD---QPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLKLRMLNEIKNYEESD---IRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKI-KSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFKLQNPSSFHKAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYR------FGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRIANSLDLQ 6131
L I+ L HK+K + +R+ KL K +E+ Q + ++EN ++ ++P+ + + + QI K+ + + + L +K F + D + + L + I + ER R + A + K F +S+ L WL+ + +++ D P ++ L + K++ K L + + T R G LK++ T+ D ++ L ML LR KW+ I V + L L+ +G +A++ + W+ D + GD+ V LID + + E+ LK R ++ + +SD +R + E+ ++ + L +L+ A+ F + + + L +AEK L+ +LP +E L LI +H+ +++++ ++ + ++G+ IL CHP + + + + I +R+ ++ + + +L +L L ++ L+EE++ + + + + +P +++++ +I++H F+ E+ +++ + K +K + PS H + R K S + S + N R+ L ++ ++W +++E + LQ+ L L E ++ FDF+ W+K ++ ++ K R+ D FRR + G +EF + I+ S FPTN+ + V +IF+++ + I Y +FI +L P + + + I DK I D + ++VA C CT R+ V++ G+NKYR FG + +L +VR L STVMVR+GGGW + EFL KNDPCR +L+
Sbjct: 2431 LKYGIQDLEHKWKIVGNKLNERRAKLETALKTAIEFHNSLQDFIGWLTQMEN-NLNTLSPASLILDSVLFQIDEHKMLANEVNTHREQIIELDKTGTHLKYFSQKQDVALIKNLLLSVQTRWEHIMQSTLERGRSLDDARKRAKQFHESWTKLMDWLEGAEKGLDHELDCGNDPERIKLMLGRHKDIQKSLGAKQPIYDSTTRTGRTLKERATLVDDDQR-LDNMLSELRDKWDTICGKSVERQHKLEEALLFSGQFAEALQVLIDWLYKVEPHLSEDQPVHGDVALVMTLIDAHKVFQRELGKRASSVNALK-RSARDLLDSSKSDASWVRAQMAELSARWDTVCKLSLSKQARLDNALKQAEVFRTLVQSIMDVLAEAEKTLRFHGTLPD--DEDSLRLLIAQHKEFMERLEAQRSEVIRAVSMGEAILAVCHPDSASAIKQWINIIRTRFEEVMTWARQHQTRLEAALSELVSNSQLLEEMLAWLQKAQEMLAQRDQETLPEQIESLHYLISEHQGFMEEMMSKQPEMEQMTKSYK-RKPSESHALFDR---TRGSTRKRGGPQSTAPASPSQPLETKNPRITLLFNRWRQVWLVALERQRKLQDALERLQELKEFENFDFDVWRKRYMQWMNHKKSRVMDFFRRMDKDQDGKITRQEFIDGILSSRFPTNRLEMTAVADIFDRDGDGYIDYYEFIAALHPNKDAY--RPITDADK------IEDEVTRQVAACKCTRRFQVEQIGENKYRFYLGNQFGDSQQLRLVRILRSTVMVRVGGGWMALDEFLVKNDPCRAKGRTNLE 3220
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006484.1 (pep scaffold:Pmarinus_7.0:GL476558:220668:227717:-1 gene:ENSPMAG00000005854.1 transcript:ENSPMAT00000006484.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 107.457 bits (267), Expect = 1.792e-24 Identity = 66/213 (30.99%), Postives = 106/213 (49.77%), Query Frame = 3
Query: 5502 FNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYR----------FGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRI 6110
++++W ++E + L++ L + +F F W+K F K++ RL D+FRR + G +EF I G + ++ L V F + Y F+ L P S+ + DK I D + ++V+ C C R+ V++ +NKYR FG + +L +VR L STVMVR+GGGW ++ +FL+KNDPCR
Sbjct: 28 WHQVWLAALERQGRLRDALQQSQTVSEQEKFSFAQWRKLFSQQTKLRKSRLMDIFRRLDGDQDGRITRDEFVREISLLGLGSEEWELDAVVGRFGVQEGTLVDYYQFVSELCP---SKAGGTMTDADK------IDDEVIRKVSACKCPQRFQVEQVEENKYRVSFTCGCRSQFGDSQQLRLVRILRSTVMVRVGGGWLSLGDFLQKNDPCRA 231
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006481.1 (pep scaffold:Pmarinus_7.0:GL476558:220668:230205:-1 gene:ENSPMAG00000005854.1 transcript:ENSPMAT00000006481.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 102.834 bits (255), Expect = 2.104e-22 Identity = 93/367 (25.34%), Postives = 160/367 (43.60%), Query Frame = 3
Query: 5091 QINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIK--DFKLQNPSSFHKAPTKRYHDRVEFYK--NIWRNSKDSLNKSTDDLST--NTRLNFLNTQFNKLWKLSIEHKVALQNHL-----------MDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYR----------FGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRI 6110
Q++ R+ L+ SL L L EE+ + E + + + +P D +++ +I++H + + ++ L + KL + S P + D+ K +S S S LS + + L ++++W ++E + L++ L + +F F W+K F K++ RL D+FRR + G +EF I G + ++ L V F + Y F+ L P S+ + DK I D + ++V+ C C R+ V++ +NKYR FG + +L +VR L STVMVR+GGGW ++ +FL+KNDPCR
Sbjct: 3 QVSGRAWRLQAWLDTSLRELEEQAHLAEEMFLWLQEAQDSLAEKETELLPQDEESIQGLIDEHQSLMERMMLKQPEMDRLGRYNRRKLSSESQL-SGPLSPFLDKTRTRKLSGSQPDSPVSSPLSPGPLSEPRDPHVTALVACWHQVWLAALERQGRLRDALQQSQTVSEQVTASCKSTSGVEKFSFAQWRKLFSQQTKLRKSRLMDIFRRLDGDQDGRITRDEFVREISLLGLGSEEWELDAVVGRFGVQEGTLVDYYQFVSELCP---SKAGGTMTDADK------IDDEVIRKVSACKCPQRFQVEQVEENKYRVSFTCGCRSQFGDSQQLRLVRILRSTVMVRVGGGWLSLGDFLQKNDPCRA 359
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Sea Lamprey
Match: GAS2L1 (growth arrest specific 2 like 1 [Source:HGNC Symbol;Acc:HGNC:16955]) HSP 1 Score: 65.855 bits (159), Expect = 1.087e-10 Identity = 31/74 (41.89%), Postives = 45/74 (60.81%), Query Frame = 3
Query: 5937 CTCTLRYYVKREGDNKYRFGQNSKLYMVR-YLNSTVMVRIGGGWQTITEFLKKNDPCRIANSLDLQKSKSMKMS 6155
CTC +++ + R + KYR G +S L VR L VMVR+GGGW T+ +L K+DPCR L S+S+ ++
Sbjct: 198 CTCPVQFPMVRVSEGKYRVGDSSSLIYVRQILRKHVMVRVGGGWDTLEHYLDKHDPCRC-----LAPSESLPLT 266
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Sea Lamprey
Match: GAS2L1 (growth arrest specific 2 like 1 [Source:HGNC Symbol;Acc:HGNC:16955]) HSP 1 Score: 65.4698 bits (158), Expect = 1.112e-10 Identity = 31/74 (41.89%), Postives = 45/74 (60.81%), Query Frame = 3
Query: 5937 CTCTLRYYVKREGDNKYRFGQNSKLYMVR-YLNSTVMVRIGGGWQTITEFLKKNDPCRIANSLDLQKSKSMKMS 6155
CTC +++ + R + KYR G +S L VR L VMVR+GGGW T+ +L K+DPCR L S+S+ ++
Sbjct: 196 CTCPVQFPMVRVSEGKYRVGDSSSLIYVRQILRKHVMVRVGGGWDTLEHYLDKHDPCRC-----LAPSESLPLT 264
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Nematostella
Match: EDO37706 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SF35]) HSP 1 Score: 213.001 bits (541), Expect = 3.529e-55 Identity = 190/748 (25.40%), Postives = 340/748 (45.45%), Query Frame = 3
Query: 4143 RIRIFNQAYREDKLFSDSYANLNAWLDESYVMIENFKD---QPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEM-LKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNL---KLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKL-NELDVWLRKAEKLLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCD-MQNVNDIINKHLIFINELSEIDSQIKSLIKDFKLQNPSSFHKAPTKRY--HDRVEFYKNIWRNSKDSLNKSTDDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLR----PERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYR---------FGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRIAN--SLDLQK--------SKSMKMSFSXXXXXXXXXXXXDEKPTVKKSIQYQKASSLSSRIPTGLSKK 6284
R R ++ YR F +L LD + ++N + P + L K K L A T++ G L D+ ED I +E + L+A+W+ + V + L L+ TG DA++ + W+ + ++GD +TV++LID + E+ + +++++ M+NE K E + + + ++ ++ + L+ +L LAKEF+S + EL + + E L ++ P ++ ++ E +EKH ++K+ + + + G+VIL CHP A + + + + RW+++ S R +L E L L L+ EL+ + + + + IP D + V+ ++ +H +F E+++ L K K + S + P + R I R+S + + N ++ L+ ++ LW LS+E LQ L + K +F+F WK F ++ R+ D+FRR R G E+F ++ + FPT ++ ++ V + F +N I Y++F+++L+ PE+ K IH+ I+++V C C ++ + G+NKYR FG + KL ++R L STVMVR+GGGW+T+ EFL+KNDPCR +LDL+ ++SM S + + KPT + S +SL + P+G K
Sbjct: 708 RKRSLDENYRMSSKFFGGVEDLMKMLDAAEEALKNEEPIGVDPAHLRTQLKKHKEFQTMLGANQAGLDATVKMGKQLMDRSLDED---AITIEGKIADLKARWDAVCGLSVERQQKLEEALLFTGMFQDALQSLLDWLFAVEPSLSTETAVMGDPETVQILIDNHKTFQRELGRREANYQSVMKAGRAMINENKVEEPAALEEKLADLRMRWDAVYAMSNTKQDRLENALVLAKEFDSSVKTELHILKQFEENLRELG--PIAEDLEEIKEQMEKHRVFHDELKQEEVNVQSAVKKGQVILRFCHPSAIQIIQQWLARLKKRWDEVWKWSSQRLMRLEEELRKLAEEDELMGELLRWIQTKEEALAEKEKEPIPDDDYEEVHRLLEEHKVFQEEMAKKQPSYDRLTKSAKRRTNGSTTRTPEVKATPSRRGGAVSRIPRSSPAGRS------AHNPAVSHLSKRWQHLWLLSMERLRRLQEKLERIAIKRASAKFNFPEWKSRFNKWLHDSKSRVQDIFRRMDHDRDGKLTREQFITGVLNTSFPTERWEMEIVASKFERNGL--IDYKEFMNALKDKPSPEK------------PKTDAQKIHNEIDRQVEHCCCAKQFSYFKVGENKYRVSHAKKERYFGDSQKLRLLRILRSTVMVRVGGGWETLQEFLEKNDPCRAEGRTNLDLRAQLVGDAGFAESMATFKSKRPSLSREGSDIGGKPTRESS-----GTSLRGKPPSGARPK 1425
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Nematostella
Match: EDO27804 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T889]) HSP 1 Score: 68.9366 bits (167), Expect = 2.605e-14 Identity = 28/57 (49.12%), Postives = 36/57 (63.16%), Query Frame = 3
Query: 5937 CTCTLRYYVKREGDNKYRFGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCR 6107
CTC RY V+R D KY+ G + L VR + VMVR+GGGW T+ + K+DPCR
Sbjct: 1 CTCPTRYPVQRIADGKYKMGDSKNLIFVRVMRKHVMVRVGGGWDTLDRYFDKHDPCR 57
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Nematostella
Match: EDO33092 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7ST60]) HSP 1 Score: 70.4774 bits (171), Expect = 6.803e-13 Identity = 28/57 (49.12%), Postives = 36/57 (63.16%), Query Frame = 3
Query: 5937 CTCTLRYYVKREGDNKYRFGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCR 6107
CTC RY V+R D KY+ G + L VR + VMVR+GGGW T+ + K+DPCR
Sbjct: 196 CTCPTRYPVQRIADGKYKMGDSKNLIFVRVMRKHVMVRVGGGWDTLDRYFDKHDPCR 252
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Nematostella
Match: EDO29908 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T2A8]) HSP 1 Score: 53.1434 bits (126), Expect = 1.556e-6 Identity = 75/390 (19.23%), Postives = 164/390 (42.05%), Query Frame = 3
Query: 4215 WLDESYVMIENFKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTL-----------RYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDL--KLLGDLDTVEVLIDECHMTENEINNHKEDFKNLKLRMLNEIKNYEESD---IRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIK 5336
WLDE + + T+ L + +QL A FH L + GS + ++C ED E ++ L+ + +W+++ + L L++ G A + + W+ + + L K+ GD+ +E+ + + + +N+I +H+ +L R + + E+D + ++E++ + + QR L+++L+ + F S++ EL WL +A + LK + + E + + + KH+ L + + + + I C Y+ ++ RW ++ + S R + L+E+ +E+L+ + ++NE I + D ++ + KH F L + + + +K
Sbjct: 1030 WLDELELKLSEVPPVGTE-------LATVQEQLKLHKA-FHAELTPHQSEITQINQLGSTVGERCRHEDSE--LVHAQLEEVNHRWDELCNHSTGRQQKLEEALLQLGQFQLAFQELLVWLRQTDSTLDEQLAKKVQGDVKYIEIELAKHKILQNDILSHEPSVDSLS-RAASALLESGETDSTALEGKVQELKTTWDAVLAKSAQRQAELEKALKDSHSFMSQIKELRGWLNEASEFLKSRRAIGGRPE-NATKQLNKHKELKEDIDAHRDPYKGMRLLAHKISDVCIKEDGVYIEGIMSGLEERWKELLTTSSARKRSLDENYRMSSKFFGGVEDLMKMLDAAEEALKNEEPIGV--DPAHLRTQLKKHKEFQTMLGANQAGLDATVK 1405
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Medaka
Match: ENSORLT00000046669.1 (pep primary_assembly:ASM223467v1:15:24337939:24343724:-1 gene:ENSORLG00000026707.1 transcript:ENSORLT00000046669.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 174.481 bits (441), Expect = 9.658e-45 Identity = 139/568 (24.47%), Postives = 274/568 (48.24%), Query Frame = 3
Query: 4122 IQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIEN---FKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLK--LRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKI-KSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFKLQNPSS---------FHKAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDL---STNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQV 5771
+QK + ER R+ + A + K F +++ L WLDES +++ + P K+ L + K K L +++ + T R G LKDK ++ D + L +ML LR KW+ + V ++ L L+ +G DA++ + W+ + D + GD+D V LID + + E+ + LK R L E + + S ++ ++E+ ++ N + L+++L A+EF+S ++ L WL +AE+ L+ SLP E L+ IE+H+ +K+++E + ++ ++G+ I++ CHP + + +R+ ++ + + ++LN +L L + L+E L+ + I N+ +P +++ + +I +H +F+ E++ + + K K + + +K T R R+ ++ ST + N R+N L +++ +W L+++ + L + L L E ++ FDF+ W+K ++ ++ K R+ D FRR + G +EF I+ S FPT++ + V
Sbjct: 50 VQKSV-ERGRLLDDARKRTKQFHETFNKLMEWLDESERTLDSEVEIANDPDKIKTQLAQHKEFQKALGSKHSVYDTTARTGRALKDKTSLRDDHQK-LDDMLCELRDKWDTVCGKSVERQNKLEEALLFSGQFTDALQALIDWLYKVEPQLAEDQPVHGDIDLVLHLIDNHKVFQKELGKRTVGVQALKRSARELIENTHDDSSWVKVQMQELSTRWETVCNLSVSKQGRLEQALCQAEEFHSTVHILLEWLAEAEQSLRFHGSLP--DEEETLLGQIEQHKEFMKKLEEKRVSVNKATSMGEAIISICHPDSITTIKHWNTIIKARFEEVQAWARQHQQRLNLALKELLATQELLETLLNWLQWAEANISNKDMEILPQEIEEIKTLIAEHQVFMEEMTRKQPDVDKITKTHKRKAAAEPPIQSQIPVLNKGRTGRK-----------RSPTQAMYASTTQTPIETKNPRVNLLVSKWQHVWLLALDRRRKLNDALDRLEELKEFANFDFDVWRKRYMRWMNHKKSRVMDFFRRIDKDQDGKVTRQEFIEGILSSKFPTSRLEMSAV 602
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Medaka
Match: macf1b (microtubule actin crosslinking factor 1b [Source:ZFIN;Acc:ZDB-GENE-100922-36]) HSP 1 Score: 116.316 bits (290), Expect = 1.690e-28 Identity = 67/187 (35.83%), Postives = 108/187 (57.75%), Query Frame = 3
Query: 5571 EKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYRFGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRIANSLDLQ 6131
+ E+ FDFN W+K ++ +I R+ D+FR + G +EF + ++ S FPTN + V NIF+ N + I Y +F+ +L P R K ++ + I++ + ++V+ C+C R+ V++ N+YRFG + +L MVR L ST+MVR+GGGW + EFL KNDPCR+ +L+
Sbjct: 2 QMEEFANFDFNIWRKRYIQWISHMKSRILDVFRCIDRDQDGRISHKEFVDYVLAS-FPTNSLEMNAVANIFDTNADGFIDYYEFVSALHPSR-DPYRKTLDADQ-------INEEVSRQVSQCSCPKRFQVEQISANRYRFGDSQQLRMVRILRSTLMVRVGGGWTALDEFLVKNDPCRVKGRTNLK 179
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Medaka
Match: ENSORLT00000034832.1 (pep primary_assembly:ASM223467v1:11:16837571:16849096:-1 gene:ENSORLG00000026542.1 transcript:ENSORLT00000034832.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 104.375 bits (259), Expect = 4.086e-23 Identity = 63/167 (37.72%), Postives = 94/167 (56.29%), Query Frame = 3
Query: 5649 RLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYRF------GQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRIANSLDLQ 6131
R+ D FRR + G +EF + I+ S FPT+K + V +IF+++ + I Y +F+ +L P + + DK I D + ++VA C C R+ V++ G+NKYRF G + +L +VR L STVMVR+GGGW + EFL KNDPCR +L+
Sbjct: 10 RVMDFFRRIDKDQDGKITRQEFIDGILASKFPTSKLEMTAVADIFDRDGDGYIDYYEFVAALHPNK--DAYRPTTDADK------IEDEVTRQVAQCKCAKRFQVEQIGENKYRFFLGNQFGDSQQLRLVRILRSTVMVRVGGGWMALDEFLVKNDPCRARGRTNLE 168
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Medaka
Match: gas2l1 (growth arrest specific 2 like 1 [Source:NCBI gene;Acc:101154873]) HSP 1 Score: 68.5514 bits (166), Expect = 8.683e-11 Identity = 29/80 (36.25%), Postives = 49/80 (61.25%), Query Frame = 3
Query: 5874 LNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYRFGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRIA 6113
L D + + ++ +I+ K C+C ++ + + + KY+ G +S L +R L + VMVR+GGGW T+ +L K+DPCR A
Sbjct: 225 LCDMRNLDELVREILGK----CSCPAQFPMIKVSEGKYKVGDSSALIFIRVLRTHVMVRVGGGWDTLEHYLDKHDPCRCA 300
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Medaka
Match: gas2b (growth arrest-specific protein 2-like [Source:NCBI gene;Acc:101166700]) HSP 1 Score: 64.6994 bits (156), Expect = 2.290e-10 Identity = 30/70 (42.86%), Postives = 43/70 (61.43%), Query Frame = 3
Query: 5901 IIHDIIEKEVAVCTCTLRYYVKREGDNKYRFGQNSKLYMVRYLNST-VMVRIGGGWQTITEFLKKNDPCR 6107
I+ IIE C+C R+ V+++ YR G K+ +R LN VMVR+GGGW+T +L+K+DPCR
Sbjct: 189 IVRGIIENPP--CSCPTRFSVEKQPKGSYRVGD--KVLYIRMLNERHVMVRVGGGWETFMGYLQKHDPCR 254
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Planmine SMEST
Match: SMESG000017519.1 (SMESG000017519.1) HSP 1 Score: 2978.35 bits (7720), Expect = 0.000e+0 Identity = 1520/1536 (98.96%), Postives = 1529/1536 (99.54%), Query Frame = 3
Query: 1257 MDLPKNWDTNVSDIKNRIDEIKIYLDNLHIMEKDLQGIDKALSEDLSDQLEINFELLEKAITNIQYCEKERLLTYTDLDKIYNIILNNYLPTIITYENNKQLAESIITETNISLEEFIKDFXXXXXXXXXXXXPFVIDAIKYRKSNLQKLENILYEIEPLVEAVESYGFDLIEQLIELTSDYGSDCSFLQSIKDDLQEIRNKFRTLKNQIADLDSKIIELLSPNSKSHILEKLTSEYEIWHSLNDVLIKLDPVQSFPEKFNKQFEEVSFLYDQVKKKEIAFHPLKQAINKLLLDDNMSCTQDDIKEQFDGVGIDNEAVLSNDADDLKDHLKEVECLIESVFYKVDNRMSILNELKDLVNLYWSEISEIFSSIKHIQKNAMSIEAFPYNKDDKNKILEPQIAHSDSISTLTDADNKSTTSSVGFELDEKFDEDSKLELPNLTESKFPEEIISQDLVKQQYSSLKKMGKDVTQFVEKLETDLVPKANQIVKIGGQFCELEISEFTESLLEEIGITEDAIEEKDILFNKALYFVDFLSDEIKKVFEWFEVMENRLINFGEITPDQKLIEINLLELKSLNTEIFSKAQQLDKIGSAISEILSYPYVPNKNTLEEMKLNIKEKYSELVKGIIEKHNDLQKKYSDIGELDFSLDDNIGILQEFENQIKNLTPQSCKGDPEYTFSCFKNLKFLDDEFNGLQSDFQNLCERISAHGDIEDNTNLRCRGTLSIFGKLKEMKILTEQIRVLIPEKYVMIQEILKNEKLLSKEIHLHIDXXXXXXXXXXXXXXXGFSQESVEQSNLIFSNYIGDLNKQRNSIENSLKLASSHIESCQYDKCTFLSTAIKTLFHKYKRCQHLAAKRKEKLSIYSKLILEYDADKQTLQKDILELENLSIKRMNPSKITVTCAEQIKSIKISIQTADKNFDIYEHLTIRSFDIKCFGNRIDNSQLILELKEYGLKLAKIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIENFKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLKLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSLPKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFKLQNPSSFHKAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDK 5864
MDLPKNWDTNVSDIKNRIDEIKIYLDNL IMEKDLQGIDKALSEDLSDQLEINFELLEKAITNIQYCEKERLLTYTDLDKIYNIILNNYLPTIITYENNKQLAESIITETNISLEEFIKDFNNYTTYTNDNETPFVIDAIK RKSNLQKLENILYEIEPLVEAVESYGFDLIEQLIELTSDYGSDCSFLQSIKDD+QEIRNKFRTLKNQIADLDSKIIELLSPNSKSHILEKLTSEYEIWHSLNDVLIKLDPVQSFPEKFNKQFEEVSFLYDQVKKKEIAFHPLKQAINKLLLDDNMSCTQDDIKEQFD +GIDNEAVLSNDADDLKDHLKEVECLIESVFYKVDNRMSILNELKDLVNLYWSEISEIFSSIKHIQKNAMSIEAFPYNKDDKNKILEPQI HSDSISTLT+ADNKSTTSSV FELDEKFDEDSKLELPNLTESKFPEEIISQDLVKQQYSSLKKMGKDVT+FVEKLETDLVPKANQIVKIGGQFCELEISEFTESLLEEIGITEDAIEEKDILFNKALYFVDFLSDEIKKVFEWFEVMENRLINFGEITPDQKLIEINLLELKSLNTEIFSKAQQLDKIGSAISEILSYPYVPNKNTLEEMKLNIKEKYSELVKGIIEKHNDLQKKYSDIGELDFSLDDNIGILQEFENQIKNLTPQSCKGDPEYT+SCFKNLKFLD+EFNGLQSDFQNLCE+ISAHGDIEDNTNLRCRGTLSIFGKLKEMKILTEQIRVLIPEKYVMIQEILKNEKLLSKEIHLHIDYLEKLNNYLKLPPKLGFSQESVEQSNLIFSNYIGDLNKQRNSIENSLKLASSHIESCQYDKCTFLSTAIKTLFHKYKRCQHLAAKRKEKLSIYSKLILEYDADKQTLQKDILELENLSIKRMNPSKITVTCAEQIKSIKISIQTADKNFDIYEHLTIRSFDIKCFGNRIDNSQLILELKEYGLKLAKIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIENFKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLKLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNS+LNELDVWLRKAEKLLKIKSLPKFQNEADLVELIEKHE LVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCD+QNVNDIINKHLIFINELSEIDSQIKSLIKDFKLQNPSSFHKAPTKRYHDRVEFYKNIWRNSKDSLNKS DDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVFNIFNKNNNNEIPYQDFIDSLRPERRSQVDK
Sbjct: 1 MDLPKNWDTNVSDIKNRIDEIKIYLDNLQIMEKDLQGIDKALSEDLSDQLEINFELLEKAITNIQYCEKERLLTYTDLDKIYNIILNNYLPTIITYENNKQLAESIITETNISLEEFIKDFNNYTTYTNDNETPFVIDAIKDRKSNLQKLENILYEIEPLVEAVESYGFDLIEQLIELTSDYGSDCSFLQSIKDDIQEIRNKFRTLKNQIADLDSKIIELLSPNSKSHILEKLTSEYEIWHSLNDVLIKLDPVQSFPEKFNKQFEEVSFLYDQVKKKEIAFHPLKQAINKLLLDDNMSCTQDDIKEQFDDIGIDNEAVLSNDADDLKDHLKEVECLIESVFYKVDNRMSILNELKDLVNLYWSEISEIFSSIKHIQKNAMSIEAFPYNKDDKNKILEPQIVHSDSISTLTEADNKSTTSSVDFELDEKFDEDSKLELPNLTESKFPEEIISQDLVKQQYSSLKKMGKDVTEFVEKLETDLVPKANQIVKIGGQFCELEISEFTESLLEEIGITEDAIEEKDILFNKALYFVDFLSDEIKKVFEWFEVMENRLINFGEITPDQKLIEINLLELKSLNTEIFSKAQQLDKIGSAISEILSYPYVPNKNTLEEMKLNIKEKYSELVKGIIEKHNDLQKKYSDIGELDFSLDDNIGILQEFENQIKNLTPQSCKGDPEYTYSCFKNLKFLDEEFNGLQSDFQNLCEKISAHGDIEDNTNLRCRGTLSIFGKLKEMKILTEQIRVLIPEKYVMIQEILKNEKLLSKEIHLHIDYLEKLNNYLKLPPKLGFSQESVEQSNLIFSNYIGDLNKQRNSIENSLKLASSHIESCQYDKCTFLSTAIKTLFHKYKRCQHLAAKRKEKLSIYSKLILEYDADKQTLQKDILELENLSIKRMNPSKITVTCAEQIKSIKISIQTADKNFDIYEHLTIRSFDIKCFGNRIDNSQLILELKEYGLKLAKIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIENFKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLGDLDTVEVLIDECHMTENEINNHKEDFKNLKLRMLNEIKNYEESDIRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSRLNELDVWLRKAEKLLKIKSLPKFQNEADLVELIEKHEVLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESLGSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDVQNVNDIINKHLIFINELSEIDSQIKSLIKDFKLQNPSSFHKAPTKRYHDRVEFYKNIWRNSKDSLNKSMDDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVFNIFNKNNNNEIPYQDFIDSLRPERRSQVDK 1536
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Planmine SMEST
Match: SMESG000017520.1 (SMESG000017520.1) HSP 1 Score: 535.798 bits (1379), Expect = 2.009e-154 Identity = 291/301 (96.68%), Postives = 295/301 (98.01%), Query Frame = 3
Query: 39 KALLFNNFHELFNESFPSSAKALTADIENLSSQKEDIDSLFLDVQEFILNYKVILSENQNSSMANSLQDSINKLKLKLNTMFRQIKKYLPFKKLEEVKGLSQKDMLETIDTLKSYQPDLSFAFESINKFEISLANVQAIPKSSIKALSIEEDLFSLKELFTNIFTEVELILNWLENLLSEFSARESDIQDIKFNLNXXXXXXXXXXXXXXXSDENLCRFSKKLSVLEKDLLEIEQSFKNFDKDFPLDFKEIKLFESLNLDCFDLAQKIFDLKTGFDVKLNLLSQTKTRLAEFFQISNKFNE 941
K LLFNNFHELFN SFPSSAKALTAD+ENLSSQKEDIDSLF DVQEFILNYKVILSENQNSSMANSLQDSINKLKLKLNTMFRQIKKYLPFKKLEEVKGLSQKDMLETIDTLKS+QPDLSFAFESINKFEISLANVQAIPKSSIKALSIEEDLFSLKELFTNIFTEVELILNWLENLLSEFSARE+DIQDIKFNLNEFEIKFIEE KFI ISDENLCRFSKKLSVLEKDLLEIEQSFKNFDKDFPL+FKEIKLFESLNLDC DLAQKIFDLKTGFDVKLNLLSQTKTRLAEFFQISNKFNE
Sbjct: 2926 KHLLFNNFHELFNVSFPSSAKALTADMENLSSQKEDIDSLFHDVQEFILNYKVILSENQNSSMANSLQDSINKLKLKLNTMFRQIKKYLPFKKLEEVKGLSQKDMLETIDTLKSHQPDLSFAFESINKFEISLANVQAIPKSSIKALSIEEDLFSLKELFTNIFTEVELILNWLENLLSEFSARENDIQDIKFNLNEFEIKFIEETKFICISDENLCRFSKKLSVLEKDLLEIEQSFKNFDKDFPLNFKEIKLFESLNLDCIDLAQKIFDLKTGFDVKLNLLSQTKTRLAEFFQISNKFNE 3226 HSP 2 Score: 32.7278 bits (73), Expect = 2.009e-154 Identity = 16/21 (76.19%), Postives = 16/21 (76.19%), Query Frame = 1
Query: 1 DEISSEFKILSQEKHYYLTTF 63
DEISSEFKILSQEKH F
Sbjct: 2913 DEISSEFKILSQEKHLLFNNF 2933
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Planmine SMEST
Match: SMESG000017520.1 (SMESG000017520.1) HSP 1 Score: 535.798 bits (1379), Expect = 2.025e-154 Identity = 291/301 (96.68%), Postives = 295/301 (98.01%), Query Frame = 3
Query: 39 KALLFNNFHELFNESFPSSAKALTADIENLSSQKEDIDSLFLDVQEFILNYKVILSENQNSSMANSLQDSINKLKLKLNTMFRQIKKYLPFKKLEEVKGLSQKDMLETIDTLKSYQPDLSFAFESINKFEISLANVQAIPKSSIKALSIEEDLFSLKELFTNIFTEVELILNWLENLLSEFSARESDIQDIKFNLNXXXXXXXXXXXXXXXSDENLCRFSKKLSVLEKDLLEIEQSFKNFDKDFPLDFKEIKLFESLNLDCFDLAQKIFDLKTGFDVKLNLLSQTKTRLAEFFQISNKFNE 941
K LLFNNFHELFN SFPSSAKALTAD+ENLSSQKEDIDSLF DVQEFILNYKVILSENQNSSMANSLQDSINKLKLKLNTMFRQIKKYLPFKKLEEVKGLSQKDMLETIDTLKS+QPDLSFAFESINKFEISLANVQAIPKSSIKALSIEEDLFSLKELFTNIFTEVELILNWLENLLSEFSARE+DIQDIKFNLNEFEIKFIEE KFI ISDENLCRFSKKLSVLEKDLLEIEQSFKNFDKDFPL+FKEIKLFESLNLDC DLAQKIFDLKTGFDVKLNLLSQTKTRLAEFFQISNKFNE
Sbjct: 2925 KHLLFNNFHELFNVSFPSSAKALTADMENLSSQKEDIDSLFHDVQEFILNYKVILSENQNSSMANSLQDSINKLKLKLNTMFRQIKKYLPFKKLEEVKGLSQKDMLETIDTLKSHQPDLSFAFESINKFEISLANVQAIPKSSIKALSIEEDLFSLKELFTNIFTEVELILNWLENLLSEFSARENDIQDIKFNLNEFEIKFIEETKFICISDENLCRFSKKLSVLEKDLLEIEQSFKNFDKDFPLNFKEIKLFESLNLDCIDLAQKIFDLKTGFDVKLNLLSQTKTRLAEFFQISNKFNE 3225 HSP 2 Score: 32.7278 bits (73), Expect = 2.025e-154 Identity = 16/21 (76.19%), Postives = 16/21 (76.19%), Query Frame = 1
Query: 1 DEISSEFKILSQEKHYYLTTF 63
DEISSEFKILSQEKH F
Sbjct: 2912 DEISSEFKILSQEKHLLFNNF 2932
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Planmine SMEST
Match: SMESG000043809.1 (SMESG000043809.1) HSP 1 Score: 211.846 bits (538), Expect = 3.008e-54 Identity = 184/693 (26.55%), Postives = 323/693 (46.61%), Query Frame = 3
Query: 4119 KIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIE---NFKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLG-DLDTVEVLIDECHMTENEINNHKEDFKNLKL-RMLNEIKNYEESD-IRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSL-PKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESL---------------------GSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFKLQNPSSFHKAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYRFGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRIA 6113
K+ R ER R + Y+E K F D L + + ++E + P+K+ + + K K L + T R G L+DK ++D +K + E++ L+ KW + + + LN L+ +GN DAM + W+ K K G D++TV+ L++E + E ++ + + + M I++ E+S I + + E+++ ++ ++ + L+E+ A EF N L + K+E L+ ++ P+ + E + +I H K + + + +F G IL + +P A + I S+W+ + S SE R L + L G+L S + EL Y + E K Q N+H FI +++ +I + K + P P R+E + I +K + L N ++N L++++ ++W + + + L +H+ L E E ++ F+F W++ +++++ R+ D FRRQ + G +EF + II S F T + ++ V +IF+K + I Y+ F+ +LRP+R + K N N+ IIHD ++K+++ CTC+ Y + + GD KY+FG KL +VR L S VMVRIGGGW + EFL K+DPCR A
Sbjct: 7491 KVVSRSSERSRNLDYGYKEAKRFEDMNRTLLDDIASNLEILERDTSIGQTPSKIREQITKHKEFHCNLGVHNLTYDSTRRLGYQLRDKAPIQDSKK--IDEIVHNLQMKWQSLCNKSIEREKALNDALLLSGNYKDAMTNLLSWLRAIEPKLSDSSKRCGGDMNTVQTLLNEHSILEKQLRDRDHMIEKINTDAMALMIQSEEDSSFIEDQLAELKSTWNNVNKLANEKKRRLEEAKNQAIEFERLWNSLKEFFSKSEHELRQFTISPEEEKEELISNMISNHGIFHKNLLMNEMKINDFQQFGHEILVSAYPEAEGSIKHYINIIKSQWSDLLSWSEQRGLNLKDHLDRLNERKILLDDLLLWLAAAEGTLLASES--RELSYALAEA--KFQ-----------------YNEHERFITDMTLKQKDHDEIITNSKKKTPPLI---PIFDRSKRMETNQGI--------SKIQEPLFVNPKINQLHSKWKQVWFMILNRRKRLLDHIEYLKEVENMKNFNFEDWRRRYMSWMNHSKSRVMDFFRRQDTDHDGRVTRKEFIDGIIDSKFQTTRVEMEVVADIFDKEKSGFIDYRQFLAALRPDRETYQVKPANENE------IIHDEVKKQISKCTCSNPYKITQCGDGKYKFGDTEKLRLVRILRSNVMVRIGGGWCALDEFLVKHDPCREA 8143
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Planmine SMEST
Match: SMESG000043809.1 (SMESG000043809.1) HSP 1 Score: 211.846 bits (538), Expect = 3.163e-54 Identity = 184/693 (26.55%), Postives = 323/693 (46.61%), Query Frame = 3
Query: 4119 KIQKRLRERIRIFNQAYREDKLFSDSYANLNAWLDESYVMIE---NFKDQPTKVLDHLDKLKNLCKQLSCQYANFHCTLRYGSYLKDKCTVEDPEKYILLEMLKTLRAKWNKIRISIVTEKSTLNRKLIKTGNIYDAMKCVNYWINDSIAKFVPDLKLLG-DLDTVEVLIDECHMTENEINNHKEDFKNLKL-RMLNEIKNYEESD-IRNTIEEIENQFSLFVNNVQQRSKSLQESLQLAKEFNSKLNELDVWLRKAEKLLKIKSL-PKFQNEADLVELIEKHEFLVKQVKEMKTTMTNFNNIGKVILTNCHPMATNYVNRLIFKTGSRWNQINSRSEDRSKKLNESL---------------------GSLRGSTALIEELIYKVGECLNKIQNEIRIEIPCDMQNVNDIINKHLIFINELSEIDSQIKSLIKDFKLQNPSSFHKAPTKRYHDRVEFYKNIWRNSKDSLNKSTDDLSTNTRLNFLNTQFNKLWKLSIEHKVALQNHLMDLNEKEQLRRFDFNTWKKDFLTYIKVKGLRLTDLFRRQQSRRPGYHVLEEFTNCIIKSGFPTNKYLLQQVXXXXXXXXXXEIPYQDFIDSLRPERRSQVDKNINLNDKKVVTLIIHDIIEKEVAVCTCTLRYYVKREGDNKYRFGQNSKLYMVRYLNSTVMVRIGGGWQTITEFLKKNDPCRIA 6113
K+ R ER R + Y+E K F D L + + ++E + P+K+ + + K K L + T R G L+DK ++D +K + E++ L+ KW + + + LN L+ +GN DAM + W+ K K G D++TV+ L++E + E ++ + + + M I++ E+S I + + E+++ ++ ++ + L+E+ A EF N L + K+E L+ ++ P+ + E + +I H K + + + +F G IL + +P A + I S+W+ + S SE R L + L G+L S + EL Y + E K Q N+H FI +++ +I + K + P P R+E + I +K + L N ++N L++++ ++W + + + L +H+ L E E ++ F+F W++ +++++ R+ D FRRQ + G +EF + II S F T + ++ V +IF+K + I Y+ F+ +LRP+R + K N N+ IIHD ++K+++ CTC+ Y + + GD KY+FG KL +VR L S VMVRIGGGW + EFL K+DPCR A
Sbjct: 7479 KVVSRSSERSRNLDYGYKEAKRFEDMNRTLLDDIASNLEILERDTSIGQTPSKIREQITKHKEFHCNLGVHNLTYDSTRRLGYQLRDKAPIQDSKK--IDEIVHNLQMKWQSLCNKSIEREKALNDALLLSGNYKDAMTNLLSWLRAIEPKLSDSSKRCGGDMNTVQTLLNEHSILEKQLRDRDHMIEKINTDAMALMIQSEEDSSFIEDQLAELKSTWNNVNKLANEKKRRLEEAKNQAIEFERLWNSLKEFFSKSEHELRQFTISPEEEKEELISNMISNHGIFHKNLLMNEMKINDFQQFGHEILVSAYPEAEGSIKHYINIIKSQWSDLLSWSEQRGLNLKDHLDRLNERKILLDDLLLWLAAAEGTLLASES--RELSYALAEA--KFQ-----------------YNEHERFITDMTLKQKDHDEIITNSKKKTPPLI---PIFDRSKRMETNQGI--------SKIQEPLFVNPKINQLHSKWKQVWFMILNRRKRLLDHIEYLKEVENMKNFNFEDWRRRYMSWMNHSKSRVMDFFRRQDTDHDGRVTRKEFIDGIIDSKFQTTRVEMEVVADIFDKEKSGFIDYRQFLAALRPDRETYQVKPANENE------IIHDEVKKQISKCTCSNPYKITQCGDGKYKFGDTEKLRLVRILRSNVMVRIGGGWCALDEFLVKHDPCREA 8131 The following BLAST results are available for this feature:
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 4
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Microtubule-actin cross-linking factor 1-like isoform X28 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30000639 ID=SMED30000639|Name=Microtubule-actin cross-linking factor 1-like isoform X28|organism=Schmidtea mediterranea sexual|type=transcript|length=6631bpback to top protein sequence of SMED30000639-orf-1 >SMED30000639-orf-1 ID=SMED30000639-orf-1|Name=SMED30000639-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=2021bp MANSLQDSINKLKLKLNTMFRQIKKYLPFKKLEEVKGLSQKDMLETIDTLback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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