ATP-dependent DNA helicase

Overview
NameATP-dependent DNA helicase
Smed IDSMED30000541
Length (bp)3540
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of ATP-dependent DNA helicase (SMED30000541) t-SNE clustered cells

Violin plots show distribution of expression levels for ATP-dependent DNA helicase (SMED30000541) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of ATP-dependent DNA helicase (SMED30000541) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for ATP-dependent DNA helicase (SMED30000541) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30000541

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30000541h1SMcG0002800 SmedASXL_017447SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30000541h1SMcG0002800 SmedASXL_017447SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of ATP-dependent DNA helicase vs. Ensembl Human
Match: BLM (BLM RecQ like helicase [Source:HGNC Symbol;Acc:HGNC:1058])

HSP 1 Score: 583.178 bits (1502), Expect = 0.000e+0
Identity = 295/650 (45.38%), Postives = 422/650 (64.92%), Query Frame = 2
Query: 1208 FPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEH-FDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVME---------ISNVGWGS--TLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQ-MPISKPRTTKDKDKVIGKEPVKEVDRLQKECYQKLVEVARKFANEAKIQNYANLFSNQTLMEMSERLPADLNDLKDITSFPQVKINKYGCNFLEVTKHYN 3118
            FPHT+ MM  F + FGL+ FR NQ EAIN+ L G D F++MPTGGGKSLCYQLPA ++ GVT+VISPL+SLI DQV KL  LDI AT+L+GD  ++E   I LQL  +   IKLLYVTPEK+  S +L++  ++L +RKLL+RFVIDEAHCVSQWGHDFR  Y+ + +LR  FP+VP++ALTATA  +V++DIL QLK+    + F  SFNR NL++ + PK  KK   + +  +       SGI+YCLSR ECD +A  + + GL   AYHAG+SD+ R +VQ+ WIN++GC+++ ATIAFGMGIDKPDVRFV+H S+PKS+EG+YQE+GRAGRDG  + C+LFY++ DV R+++LI  +  G   T++ H   L+ MV YCEN+ +CRR Q+L +FGE+ F+   C       CDNC  + D K   RD+TD  K++V++V E         I +VG     T+N ++D+F G+ +AKI       + ++G GS +S+++AER F  L+ +  L E + +   D  +A+V+ G K Q +L+GN  V  M      + K +  ++ K  V + + + K+C  +L EV +       + +Y N+F+  TL +++E L +D   L  I    + K+ KYG   + V + Y+
Sbjct:  649 FPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTVVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATNIYLQLSKKDPIIKLLYVTPEKICASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKIL-RPQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQRDGLAALAYHAGLSDSARDEVQQKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISHCLLFYTYHDVTRLKRLIMMEKDGNHHTRETHFNNLYSMVHYCENITECRRIQLLAYFGENGFNPDFCKKHPDVSCDNCCKTKDYKT--RDVTDDVKSIVRFVQEHSSSQGMRNIKHVGPSGRFTMNMLVDIFLGSKSAKI------QSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQAIAYVMLGNKAQTVLNGNLKVDFMETENSSSVKKQKALVAK--VSQREEMVKKCLGELTEVCKSLGKVFGV-HYFNIFNTVTLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVISVLQKYS 1286          
BLAST of ATP-dependent DNA helicase vs. Ensembl Human
Match: BLM (BLM RecQ like helicase [Source:HGNC Symbol;Acc:HGNC:1058])

HSP 1 Score: 582.793 bits (1501), Expect = 0.000e+0
Identity = 295/650 (45.38%), Postives = 422/650 (64.92%), Query Frame = 2
Query: 1208 FPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEH-FDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVME---------ISNVGWGS--TLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQ-MPISKPRTTKDKDKVIGKEPVKEVDRLQKECYQKLVEVARKFANEAKIQNYANLFSNQTLMEMSERLPADLNDLKDITSFPQVKINKYGCNFLEVTKHYN 3118
            FPHT+ MM  F + FGL+ FR NQ EAIN+ L G D F++MPTGGGKSLCYQLPA ++ GVT+VISPL+SLI DQV KL  LDI AT+L+GD  ++E   I LQL  +   IKLLYVTPEK+  S +L++  ++L +RKLL+RFVIDEAHCVSQWGHDFR  Y+ + +LR  FP+VP++ALTATA  +V++DIL QLK+    + F  SFNR NL++ + PK  KK   + +  +       SGI+YCLSR ECD +A  + + GL   AYHAG+SD+ R +VQ+ WIN++GC+++ ATIAFGMGIDKPDVRFV+H S+PKS+EG+YQE+GRAGRDG  + C+LFY++ DV R+++LI  +  G   T++ H   L+ MV YCEN+ +CRR Q+L +FGE+ F+   C       CDNC  + D K   RD+TD  K++V++V E         I +VG     T+N ++D+F G+ +AKI       + ++G GS +S+++AER F  L+ +  L E + +   D  +A+V+ G K Q +L+GN  V  M      + K +  ++ K  V + + + K+C  +L EV +       + +Y N+F+  TL +++E L +D   L  I    + K+ KYG   + V + Y+
Sbjct:  649 FPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTVVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATNIYLQLSKKDPIIKLLYVTPEKICASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKIL-RPQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQRDGLAALAYHAGLSDSARDEVQQKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISHCLLFYTYHDVTRLKRLIMMEKDGNHHTRETHFNNLYSMVHYCENITECRRIQLLAYFGENGFNPDFCKKHPDVSCDNCCKTKDYKT--RDVTDDVKSIVRFVQEHSSSQGMRNIKHVGPSGRFTMNMLVDIFLGSKSAKI------QSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQAIAYVMLGNKAQTVLNGNLKVDFMETENSSSVKKQKALVAK--VSQREEMVKKCLGELTEVCKSLGKVFGV-HYFNIFNTVTLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVISVLQKYS 1286          
BLAST of ATP-dependent DNA helicase vs. Ensembl Human
Match: BLM (BLM RecQ like helicase [Source:HGNC Symbol;Acc:HGNC:1058])

HSP 1 Score: 512.301 bits (1318), Expect = 4.052e-160
Identity = 245/473 (51.80%), Postives = 331/473 (69.98%), Query Frame = 2
Query: 1208 FPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEH-FDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVME---------ISNVGWGS--TLNYVLDVF 2590
            FPHT+ MM  F + FGL+ FR NQ EAIN+ L G D F++MPTGGGKSLCYQLPA ++ GVT+VISPL+SLI DQV KL  LDI AT+L+GD  ++E   I LQL  +   IKLLYVTPEK+  S +L++  ++L +RKLL+RFVIDEAHCVSQWGHDFR  Y+ + +LR  FP+VP++ALTATA  +V++DIL QLK+    + F  SFNR NL++ + PK  KK   + +  +       SGI+YCLSR ECD +A  + + GL   AYHAG+SD+ R +VQ+ WIN++GC+++ ATIAFGMGIDKPDVRFV+H S+PKS+EG+YQE+GRAGRDG  + C+LFY++ DV R+++LI  +  G   T++ H   L+ MV YCEN+ +CRR Q+L +FGE+ F+   C       CDNC  + D K   RD+TD  K++V++V E         I +VG     T+N ++D+F
Sbjct:  649 FPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTVVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATNIYLQLSKKDPIIKLLYVTPEKICASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKIL-RPQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQRDGLAALAYHAGLSDSARDEVQQKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISHCLLFYTYHDVTRLKRLIMMEKDGNHHTRETHFNNLYSMVHYCENITECRRIQLLAYFGENGFNPDFCKKHPDVSCDNCCKTKDYKT--RDVTDDVKSIVRFVQEHSSSQGMRNIKHVGPSGRFTMNMLVDIF 1118          
BLAST of ATP-dependent DNA helicase vs. Ensembl Human
Match: RECQL (RecQ like helicase [Source:HGNC Symbol;Acc:HGNC:9948])

HSP 1 Score: 387.882 bits (995), Expect = 2.598e-120
Identity = 206/561 (36.72%), Postives = 323/561 (57.58%), Query Frame = 2
Query: 1157 EYKSHPNDGETAEFSGDFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIR--PKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVMEISNVGWGSTLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISK 2833
            EY S P     A    DFP + ++    + VF L  FR  Q E IN T++G + F++MPTGGGKSLCYQLPA+ ++G T+VI PL SL+ DQ+  L++L I AT L+    +  V  +  ++ ++  ++KL+YVTPEK+ +S+  M+  +   + +  +R  +DE HC SQWGHDFRP Y+ LG+L+  FPN  +I LTATAT  V  D    L ++     F  SFNR NL +E+R  P   +  I +I+ L+N +++ QSGI+YC S+ + ++V   +   G+   AYHA +    +  V   W + N  ++VVAT+AFGMGIDKPDVRFV+H+S+ KS+E +YQE+GRAGRD + A C+L+Y + D+ R+  ++  +N G        +Q+L+ MVSYC+N+  CRR  +  HF E ++ + C      +CDNC    D+    +++T++ + ++K + +   +    T   ++D + G   AK+    G             + D E+   H + + YL E    T A   ++++  GPK  LL +    + M ++K
Sbjct:   60 EYDSSP----AAWNKEDFPWSGKVKDILQNVFKLEKFRPLQLETINVTMAGKEVFLVMPTGGGKSLCYQLPALCSDGFTLVICPLISLMEDQLMVLKQLGISATMLNASSSKEHVKWVHAEMVNKNSELKLIYVTPEKIAKSKMFMSRLEKAYEARRFTRIAVDEVHCCSQWGHDFRPDYKALGILKRQFPNASLIGLTATATNHVLTDAQKILCIEKCFT-FTASFNRPNLYYEVRQKPSNTEDFIEDIVKLINGRYKGQSGIIYCFSQKDSEQVTVSLQNLGIHAGAYHANLEPEDKTTVHRKW-SANEIQVVVATVAFGMGIDKPDVRFVIHHSMSKSMENYYQESGRAGRDDMKADCILYYGFGDIFRISSMVVMENVG--------QQKLYEMVSYCQNISKCRRVLMAQHFDEVWNSEACNK----MCDNC--CKDSAFERKNITEYCRDLIKILKQAEELNEKLTPLKLIDSWMGKGAAKL-RVAGV------VAPTLPREDLEKIIAHFLIQQYLKEDYSFT-AYATISYLKIGPKANLLNNEAHAITMQVTK 592          
BLAST of ATP-dependent DNA helicase vs. Ensembl Human
Match: RECQL (RecQ like helicase [Source:HGNC Symbol;Acc:HGNC:9948])

HSP 1 Score: 387.882 bits (995), Expect = 2.598e-120
Identity = 206/561 (36.72%), Postives = 323/561 (57.58%), Query Frame = 2
Query: 1157 EYKSHPNDGETAEFSGDFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIR--PKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVMEISNVGWGSTLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISK 2833
            EY S P     A    DFP + ++    + VF L  FR  Q E IN T++G + F++MPTGGGKSLCYQLPA+ ++G T+VI PL SL+ DQ+  L++L I AT L+    +  V  +  ++ ++  ++KL+YVTPEK+ +S+  M+  +   + +  +R  +DE HC SQWGHDFRP Y+ LG+L+  FPN  +I LTATAT  V  D    L ++     F  SFNR NL +E+R  P   +  I +I+ L+N +++ QSGI+YC S+ + ++V   +   G+   AYHA +    +  V   W + N  ++VVAT+AFGMGIDKPDVRFV+H+S+ KS+E +YQE+GRAGRD + A C+L+Y + D+ R+  ++  +N G        +Q+L+ MVSYC+N+  CRR  +  HF E ++ + C      +CDNC    D+    +++T++ + ++K + +   +    T   ++D + G   AK+    G             + D E+   H + + YL E    T A   ++++  GPK  LL +    + M ++K
Sbjct:   60 EYDSSP----AAWNKEDFPWSGKVKDILQNVFKLEKFRPLQLETINVTMAGKEVFLVMPTGGGKSLCYQLPALCSDGFTLVICPLISLMEDQLMVLKQLGISATMLNASSSKEHVKWVHAEMVNKNSELKLIYVTPEKIAKSKMFMSRLEKAYEARRFTRIAVDEVHCCSQWGHDFRPDYKALGILKRQFPNASLIGLTATATNHVLTDAQKILCIEKCFT-FTASFNRPNLYYEVRQKPSNTEDFIEDIVKLINGRYKGQSGIIYCFSQKDSEQVTVSLQNLGIHAGAYHANLEPEDKTTVHRKW-SANEIQVVVATVAFGMGIDKPDVRFVIHHSMSKSMENYYQESGRAGRDDMKADCILYYGFGDIFRISSMVVMENVG--------QQKLYEMVSYCQNISKCRRVLMAQHFDEVWNSEACNK----MCDNC--CKDSAFERKNITEYCRDLIKILKQAEELNEKLTPLKLIDSWMGKGAAKL-RVAGV------VAPTLPREDLEKIIAHFLIQQYLKEDYSFT-AYATISYLKIGPKANLLNNEAHAITMQVTK 592          
BLAST of ATP-dependent DNA helicase vs. Ensembl Celegans
Match: him-6 (Bloom syndrome protein homolog [Source:UniProtKB/Swiss-Prot;Acc:O18017])

HSP 1 Score: 483.797 bits (1244), Expect = 8.464e-153
Identity = 278/672 (41.37%), Postives = 392/672 (58.33%), Query Frame = 2
Query: 1238 FKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTF--PNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCICDNCQNSNDNKVVMR--DLTDHAKAVVKYVMEISNVGWGSTLNYVLDVFRGANNAKIVEKQ---GANNW-LYGTGSVFSKNDAERFFHHLVFEGYLWEKM--VVTQADFVVAHVVPGPKHQLLLSGNATVQ-----------------MPISKPRTTKDKDKVIGKEPVKEVDRLQKECYQKLVEVARKFANEAKIQNYANLFSNQTLMEMSERLPADLNDLKDITSFPQVKINKYGCNFLEVTKHY-NLLLTKHKQEMKDLEKKL 3169
             K  FG N FR  QK+ I STL G DTFV+MPTG GKSLCYQLPAVI  GVT+V+SPL+SLI DQ  K++EL I    L+ DL     +KI  +L S    IKLLYVTPEK+  S +L +VF  L++R LL+RFVIDEAHCVSQWGHDFRP Y  L  LR  +  P VP+IALTATAT K+  D    LKM+ N+K FI SF R NL++++ PK A+  I+ ++  +   +  +SGIVYCLSR EC+ V  ++ ++GL  + YHAG++D  R  VQ  WI  N   ++ ATIAFGMGIDKPDVRFV+HYS+PKSIEG+YQETGRAGRDG+P+ C++ YS+ D +R+R++I   N+ T   +  H   +  +V+YCEN+  CRR  ++ HFGE +D + C   K   CD C+    N   +R  D++  A +++K +  +      +TL Y+ +++RGA   K  E+    G      Y  G   S+ DA RF   LV EGY+ E++  V  QA  V A+       + L +G  T +                 + +S      +   +  +  VK  D   + C Q L  +    A  + +    ++ S + + +++  LP   +DL  I S  Q+K+ KYG   +E+   Y   +  + ++EM++   KL
Sbjct:  239 LKSKFGFNQFRHRQKQCILSTLMGHDTFVLMPTGAGKSLCYQLPAVILPGVTVVVSPLRSLIEDQKMKMKELGIGCEALTADLGAPAQEKIYAELGSGNPSIKLLYVTPEKISASGRLNSVFFDLHRRGLLARFVIDEAHCVSQWGHDFRPDYTKLSSLREKYANPPVPIIALTATATPKIVTDARDHLKMQ-NSKLFISSFVRDNLKYDLIPKAARSLIN-VVEKMKQLYPGKSGIVYCLSRKECETVQMMLTKAGLSAEVYHAGLNDNLRVSVQRSWI-ANKFDVICATIAFGMGIDKPDVRFVIHYSLPKSIEGYYQETGRAGRDGMPSYCLMLYSYHDSIRLRRMIEEGNT-TTGVRSMHLNNVLQVVAYCENVSVCRRKMLVEHFGEVYDEQSCRNSK-TPCDICERQRKNAEAIRLFDVSTDALSILKCLPRMQK----ATLKYISELYRGALIKKSQEQAMRLGHTKLPFYSKGQGMSEQDALRFVRKLVIEGYIHERLYSVPNQAAAVFAYAELTEAGRDLANGKKTAKVYLHIVTCERKRKNAGLIELSNMNIVSEAQALKERHMVKHGDVFTR-CLQDLTHLITAVAESSGLSGPYSIVSREGIEQIAALLPRTNSDLLRIDSMTQIKVTKYGRLIMELLATYWKQVDEREEEEMRNQLDKL 900          
BLAST of ATP-dependent DNA helicase vs. Ensembl Celegans
Match: K02F3.12 (Putative ATP-dependent DNA helicase Q1 [Source:UniProtKB/Swiss-Prot;Acc:Q9TXJ8])

HSP 1 Score: 367.081 bits (941), Expect = 1.950e-113
Identity = 203/560 (36.25%), Postives = 310/560 (55.36%), Query Frame = 2
Query: 1208 FPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEI--RPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCICDNCQNSND----NKVVMRDLTDHAKAVVKYVMEISNVGWGSTLNYVLDVFRGANNAKIVE-----KQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISKPRTTKDK 2854
            FP ++      KE F L  FR  Q+ AIN+ +S  D  VI+ TGGGKSLCYQLPA++  G+ +V+SPL SL+ DQ+ +L+ L I ++ L+ +  + E  ++   + ++  + +LLYVTPEK+ +S+K+MN  +       L    IDE HC SQWGHDFR  Y  L +L+  F  VP++ LTATAT+ V +D+   L ++     F   FNR NL++++  +P +  +C  EI   +   F  Q+GI+YCLSRN+C++VA  +   G+K + YHA M    R    + WI+    +++VAT+AFGMGIDKP+VRFV+H+S+PKSIE +YQE+GRAGRDG PATC+L+Y   D+ +   ++  + +G         Q L+ MV Y  +   CRR ++  HF E ++   C       CD C+N N     +     D+++ AK  V+ + E         LN   D        K+VE      +G+ N  +           E+   +L+ EGYL E    T    V+++VV G K ++    +A     + + ++ K K
Sbjct:   68 FPWSDEATKILKEQFHLEKFRPLQRAAINAVMSKEDAVVILSTGGGKSLCYQLPALLANGLALVVSPLISLVEDQILQLRSLGIDSSSLNANTSKEEAKRVEDAITNKDSKFRLLYVTPEKLAKSKKMMNKLEKSLSVGFLKLIAIDEVHCCSQWGHDFRTDYSFLNVLKRQFKGVPILGLTATATSNVLDDVKDMLGIQAALT-FRAGFNRSNLKYKVVQKPGSEDECTEEIAKTIKRDFAGQTGIIYCLSRNDCEKVAKALKSHGIKAKHYHAYMEPVDRSGAHQGWIS-GKIQVIVATVAFGMGIDKPNVRFVIHHSLPKSIENYYQESGRAGRDGQPATCILYYRLADIFKQSSMVQQERTGI--------QNLYNMVRYAADSSTCRRVKLAEHFEEAWEPSWCQKQ----CDTCENGNGFVGTSSKESTDVSEAAKTTVRIIEE--------HLNSAKDGSGRITGNKLVELLTKKLKGSRNREF----------CEKLIVNLLLEGYLQEDFHYTVYS-VISYVVIGSKWRVYNGKDAIKMRHVEESKSRKRK 594          
BLAST of ATP-dependent DNA helicase vs. Ensembl Celegans
Match: K02F3.12 (Putative ATP-dependent DNA helicase Q1 [Source:UniProtKB/Swiss-Prot;Acc:Q9TXJ8])

HSP 1 Score: 367.081 bits (941), Expect = 1.950e-113
Identity = 203/560 (36.25%), Postives = 310/560 (55.36%), Query Frame = 2
Query: 1208 FPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEI--RPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCICDNCQNSND----NKVVMRDLTDHAKAVVKYVMEISNVGWGSTLNYVLDVFRGANNAKIVE-----KQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISKPRTTKDK 2854
            FP ++      KE F L  FR  Q+ AIN+ +S  D  VI+ TGGGKSLCYQLPA++  G+ +V+SPL SL+ DQ+ +L+ L I ++ L+ +  + E  ++   + ++  + +LLYVTPEK+ +S+K+MN  +       L    IDE HC SQWGHDFR  Y  L +L+  F  VP++ LTATAT+ V +D+   L ++     F   FNR NL++++  +P +  +C  EI   +   F  Q+GI+YCLSRN+C++VA  +   G+K + YHA M    R    + WI+    +++VAT+AFGMGIDKP+VRFV+H+S+PKSIE +YQE+GRAGRDG PATC+L+Y   D+ +   ++  + +G         Q L+ MV Y  +   CRR ++  HF E ++   C       CD C+N N     +     D+++ AK  V+ + E         LN   D        K+VE      +G+ N  +           E+   +L+ EGYL E    T    V+++VV G K ++    +A     + + ++ K K
Sbjct:   68 FPWSDEATKILKEQFHLEKFRPLQRAAINAVMSKEDAVVILSTGGGKSLCYQLPALLANGLALVVSPLISLVEDQILQLRSLGIDSSSLNANTSKEEAKRVEDAITNKDSKFRLLYVTPEKLAKSKKMMNKLEKSLSVGFLKLIAIDEVHCCSQWGHDFRTDYSFLNVLKRQFKGVPILGLTATATSNVLDDVKDMLGIQAALT-FRAGFNRSNLKYKVVQKPGSEDECTEEIAKTIKRDFAGQTGIIYCLSRNDCEKVAKALKSHGIKAKHYHAYMEPVDRSGAHQGWIS-GKIQVIVATVAFGMGIDKPNVRFVIHHSLPKSIENYYQESGRAGRDGQPATCILYYRLADIFKQSSMVQQERTGI--------QNLYNMVRYAADSSTCRRVKLAEHFEEAWEPSWCQKQ----CDTCENGNGFVGTSSKESTDVSEAAKTTVRIIEE--------HLNSAKDGSGRITGNKLVELLTKKLKGSRNREF----------CEKLIVNLLLEGYLQEDFHYTVYS-VISYVVIGSKWRVYNGKDAIKMRHVEESKSRKRK 594          
BLAST of ATP-dependent DNA helicase vs. Ensembl Celegans
Match: K02F3.12 (Putative ATP-dependent DNA helicase Q1 [Source:UniProtKB/Swiss-Prot;Acc:Q9TXJ8])

HSP 1 Score: 366.696 bits (940), Expect = 5.487e-113
Identity = 203/560 (36.25%), Postives = 310/560 (55.36%), Query Frame = 2
Query: 1208 FPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEI--RPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCICDNCQNSND----NKVVMRDLTDHAKAVVKYVMEISNVGWGSTLNYVLDVFRGANNAKIVE-----KQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISKPRTTKDK 2854
            FP ++      KE F L  FR  Q+ AIN+ +S  D  VI+ TGGGKSLCYQLPA++  G+ +V+SPL SL+ DQ+ +L+ L I ++ L+ +  + E  ++   + ++  + +LLYVTPEK+ +S+K+MN  +       L    IDE HC SQWGHDFR  Y  L +L+  F  VP++ LTATAT+ V +D+   L ++     F   FNR NL++++  +P +  +C  EI   +   F  Q+GI+YCLSRN+C++VA  +   G+K + YHA M    R    + WI+    +++VAT+AFGMGIDKP+VRFV+H+S+PKSIE +YQE+GRAGRDG PATC+L+Y   D+ +   ++  + +G         Q L+ MV Y  +   CRR ++  HF E ++   C       CD C+N N     +     D+++ AK  V+ + E         LN   D        K+VE      +G+ N          +   E+   +L+ EGYL E    T    V+++VV G K ++    +A     + + ++ K K
Sbjct:   91 FPWSDEATKILKEQFHLEKFRPLQRAAINAVMSKEDAVVILSTGGGKSLCYQLPALLANGLALVVSPLISLVEDQILQLRSLGIDSSSLNANTSKEEAKRVEDAITNKDSKFRLLYVTPEKLAKSKKMMNKLEKSLSVGFLKLIAIDEVHCCSQWGHDFRTDYSFLNVLKRQFKGVPILGLTATATSNVLDDVKDMLGIQAALT-FRAGFNRSNLKYKVVQKPGSEDECTEEIAKTIKRDFAGQTGIIYCLSRNDCEKVAKALKSHGIKAKHYHAYMEPVDRSGAHQGWIS-GKIQVIVATVAFGMGIDKPNVRFVIHHSLPKSIENYYQESGRAGRDGQPATCILYYRLADIFKQSSMVQQERTGI--------QNLYNMVRYAADSSTCRRVKLAEHFEEAWEPSWCQKQ----CDTCENGNGFVGTSSKESTDVSEAAKTTVRIIEE--------HLNSAKDGSGRITGNKLVELLTKKLKGSRN----------REFCEKLIVNLLLEGYLQEDFHYTVYS-VISYVVIGSKWRVYNGKDAIKMRHVEESKSRKRK 617          
BLAST of ATP-dependent DNA helicase vs. Ensembl Celegans
Match: rcq-5 (ATP-dependent DNA helicase [Source:UniProtKB/TrEMBL;Acc:Q19046])

HSP 1 Score: 291.197 bits (744), Expect = 1.374e-83
Identity = 180/461 (39.05%), Postives = 251/461 (54.45%), Query Frame = 2
Query: 1220 ERMMIAFKEVFGLNGFR-RNQKEAINSTLS-GFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVE--SRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPK----TAK---------KCI-----SEIINLV---------NAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQ--------------LFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCICDNCQN 2467
            ER    F E+F    +R R Q +AIN  L    D +V +PTG GKSLCYQLPAV+  G+T+VISPL +L+ DQ++ L+   I    L+  L   E  +I+ +L  +   I++LY+T E V    ++KL+N    L  R +L   V+DEAHCV+QWGHDFRP Y  LG LR   P VP +ALTATA  K ++DI +QLK++ N + F     R NL +++  +    TA          KC+     +   NL          N K    S IVYC SRNEC +VA ++  +G+   AYHAG+    R +VQE W+N N   +V AT+AFGMGIDKPDVR V+H+S  +++ G+YQE GRAGRDG  + C ++YS  D   +  L+  + +   E  K++  +              L  M+ YCE+   CR   +       FD   C   K   CD C++
Sbjct:  163 ERRDKVFLELFCHKKYRSRLQMQAINCILKRKCDVYVSLPTGAGKSLCYQLPAVVHGGITVVISPLIALMKDQISSLKRKGIPCETLNSTLTTVERSRIMGELAKEKPTIRMLYLTAEGVATDGTKKLLN---GLANRDVLRYIVVDEAHCVTQWGHDFRPDYLTLGSLRDVCPGVPWVALTATANAKAQDDIAFQLKLR-NPESFKSGTYRDNLFYDVAMRDHLATAPENHMASFITKCLTVDAKTSSSNLTKHEKAERSQNKKTFTGSAIVYCRSRNECGQVAKMLEIAGIPAMAYHAGLGKKDRNEVQEKWMN-NEIPVVAATVAFGMGIDKPDVRAVIHWSPSQNLAGYYQEAGRAGRDGKRSYCRIYYSKQDKNALNFLVSGELAKLREKAKKNNAEGEKAEMQIKSIQTGLAKMLEYCES-ARCRHVSI----ASFFDDTECRPCK-TNCDYCRD 612          
BLAST of ATP-dependent DNA helicase vs. Ensembl Fly
Match: Blm (gene:FBgn0002906 transcript:FBtr0082434)

HSP 1 Score: 582.022 bits (1499), Expect = 0.000e+0
Identity = 310/729 (42.52%), Postives = 431/729 (59.12%), Query Frame = 2
Query:  971 DDVFESIDESLFIADTENFVGQLNAYRNENAPMINVTNAADLDGTSYVPINEK---QSKWAIEDFEYKSHPNDGETAEFSGD-FPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVMEISNVGWGSTLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISKPRTTKDKDKVIGK----------EPVKEVDRLQKECYQKLVEVARKFANEAKIQNYANLFSNQTLMEMSERLPADLNDLKDITSFPQVKINKYGCNFLEVTKHY 3115
            DD F   DE++F    E    Q  A ++       V++A      S VP  +     S+    +F    H NDG T EF G  F H+ R+M      FGL  FR NQ + IN+TL G D FV+MPTGGGKSLCYQLPA++TEGVTIVISPLKSLI DQ+ KL  LDI A  LSG+ K A+V  I   L+SQ   +KLLYVTPEK+  S +  +   +LN    +SRFVIDEAHCVSQWGHDFRP Y+ LG+L+  FPNVP IALTATAT +VR DIL QL +K N KWF+ SFNR NLR+ + PK     + +I   + +K +  SGI+YCLSR ECDE +  M + G++  +YHAG++DT R+  Q+ W+     +++ AT+AFGMGIDKPDVRFV+HYS+PKSIEG+YQE GRAGRDG  A C+L+Y+++D++R++K++ SD +     KK H   L+ +V YCENL DCRR Q L++FGEHF  + C   +   CDNC N    K V  D  +HA+   + V ++ +     TL ++ DV +G+   KI++        +G    + KND  R    +V +G+L E ++ T  DF  A++  G     L+ G    +  ++K    K+    +G           +    +  + + CY  L+++ R  A++  +   A++ + Q L  M+E LP    D+  I    +   +KYG   LE+T +Y
Sbjct:  647 DDGFPEYDEAMF----EQMHSQAAANKSR------VSSAGPSTSKSVVPTKQTSALHSQKLSGNFHANVH-NDGITGEFDGQKFEHSTRLMHGLSYSFGLKSFRPNQLQVINATLLGNDCFVLMPTGGGKSLCYQLPAILTEGVTIVISPLKSLIFDQINKLASLDICAKSLSGEQKMADVMAIYRDLESQPPMVKLLYVTPEKISSSARFQDTLDTLNSNNYISRFVIDEAHCVSQWGHDFRPDYKKLGVLKKRFPNVPTIALTATATPRVRLDILAQLNLK-NCKWFLSSFNRSNLRYRVLPKKGVSTLDDISRYIRSKPQHFSGIIYCLSRKECDETSKKMCKDGVRAVSYHAGLTDTDRESRQKDWLT-GKMRVICATVAFGMGIDKPDVRFVLHYSLPKSIEGYYQEAGRAGRDGDVADCILYYNYSDMLRIKKMLDSDKALQYNVKKIHVDNLYRIVGYCENLTDCRRAQQLDYFGEHFTSEQCLENRETACDNCINKRAYKAV--DALEHARKAARAVKDLCSGRSRFTLLHIADVLKGSKIKKIIDFNHHKTPHHGVLKDWDKNDVHRLLRKMVIDGFLREDLIFTN-DFPQAYLYLGNNISKLMEGTPNFEFAVTK--NAKEAKAAVGSVSDGATSSTADGQSGMREIHERCYTDLLDLCRTIASQRNV-TMASIMNIQALKSMAETLPITEKDMCSIPHVTKANFDKYGAKLLEITSNY 1356          
BLAST of ATP-dependent DNA helicase vs. Ensembl Fly
Match: RecQ5 (gene:FBgn0027375 transcript:FBtr0100362)

HSP 1 Score: 273.478 bits (698), Expect = 9.801e-81
Identity = 153/420 (36.43%), Postives = 239/420 (56.90%), Query Frame = 2
Query: 1214 HTERMMIAFKEVFGLNGFRRNQKEAINSTLSGF--DTFVIMPTGGGKSLCYQLPAVITEG-VTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKT------------AKKCISEIINLVNA-KFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSD-----NSGTIETKKQHE-QQLFLMVSYCENLIDCRRTQVLNHFGE 2407
            H   +  A K+ FG + F+ + +E           D +V MPTG GKSLC+QLP +++E  +TIV SPL +LI DQ+  L +L + A  L+  +   E D++++ L++    +K LY+TPE+   ++   ++ Q+L++   L+ F +DEAHCVSQWGHDFRP Y  LG LR+ + +V  +ALTATA+ +V+EDI  QL++      F     R NL ++I  K             A+ C+       +  K +   GIVYC +R++ + +A  + + G+   AYHAG+   +R +VQE W+  +   I+ AT +FGMG+DKP VRFV+H+ +P+++  +YQE+GRAGRDGL + C L+Y   DV  +R L+ +D       G  E   +   +Q   +  +CE    CR     + FG+
Sbjct:    3 HESAVHEALKKHFGHSKFKSDLQEKAVKCAVKKKQDVYVSMPTGSGKSLCFQLPGLMSENQITIVFSPLLALIKDQIDHLTKLKVPADSLNSKMSTKERDRVIMDLKAVRTNLKFLYITPEQAA-TKFFQDLLQTLHKHNKLAYFAVDEAHCVSQWGHDFRPDYLKLGELRSKYSDVIWLALTATASREVKEDIYKQLRLHQPVAQFSTPSFRKNLFYDIVYKNSIEDDFQHLADFARHCLGNPKEFKDTPKPQRGCGIVYCRTRDQVERMAIGVTKQGIGAVAYHAGLKTGERTEVQEAWMRGDQ-PIICATNSFGMGVDKPSVRFVIHWDVPQNVAAYYQESGRAGRDGLQSYCRLYYGREDVRSIRFLLQNDAHRARGRGDKELLTERAIKQFEKITEFCERTT-CRHKLFSDFFGD 419          
BLAST of ATP-dependent DNA helicase vs. Ensembl Fly
Match: RecQ5 (gene:FBgn0027375 transcript:FBtr0075714)

HSP 1 Score: 273.092 bits (697), Expect = 1.078e-80
Identity = 153/420 (36.43%), Postives = 239/420 (56.90%), Query Frame = 2
Query: 1214 HTERMMIAFKEVFGLNGFRRNQKEAINSTLSGF--DTFVIMPTGGGKSLCYQLPAVITEG-VTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKT------------AKKCISEIINLVNA-KFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSD-----NSGTIETKKQHE-QQLFLMVSYCENLIDCRRTQVLNHFGE 2407
            H   +  A K+ FG + F+ + +E           D +V MPTG GKSLC+QLP +++E  +TIV SPL +LI DQ+  L +L + A  L+  +   E D++++ L++    +K LY+TPE+   ++   ++ Q+L++   L+ F +DEAHCVSQWGHDFRP Y  LG LR+ + +V  +ALTATA+ +V+EDI  QL++      F     R NL ++I  K             A+ C+       +  K +   GIVYC +R++ + +A  + + G+   AYHAG+   +R +VQE W+  +   I+ AT +FGMG+DKP VRFV+H+ +P+++  +YQE+GRAGRDGL + C L+Y   DV  +R L+ +D       G  E   +   +Q   +  +CE    CR     + FG+
Sbjct:    3 HESAVHEALKKHFGHSKFKSDLQEKAVKCAVKKKQDVYVSMPTGSGKSLCFQLPGLMSENQITIVFSPLLALIKDQIDHLTKLKVPADSLNSKMSTKERDRVIMDLKAVRTNLKFLYITPEQAA-TKFFQDLLQTLHKHNKLAYFAVDEAHCVSQWGHDFRPDYLKLGELRSKYSDVIWLALTATASREVKEDIYKQLRLHQPVAQFSTPSFRKNLFYDIVYKNSIEDDFQHLADFARHCLGNPKEFKDTPKPQRGCGIVYCRTRDQVERMAIGVTKQGIGAVAYHAGLKTGERTEVQEAWMRGDQ-PIICATNSFGMGVDKPSVRFVIHWDVPQNVAAYYQESGRAGRDGLQSYCRLYYGREDVRSIRFLLQNDAHRARGRGDKELLTERAIKQFEKITEFCERTT-CRHKLFSDFFGD 419          
BLAST of ATP-dependent DNA helicase vs. Ensembl Fly
Match: RecQ5 (gene:FBgn0027375 transcript:FBtr0075715)

HSP 1 Score: 276.174 bits (705), Expect = 9.334e-77
Identity = 153/420 (36.43%), Postives = 239/420 (56.90%), Query Frame = 2
Query: 1214 HTERMMIAFKEVFGLNGFRRNQKEAINSTLSGF--DTFVIMPTGGGKSLCYQLPAVITEG-VTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKT------------AKKCISEIINLVNA-KFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSD-----NSGTIETKKQHE-QQLFLMVSYCENLIDCRRTQVLNHFGE 2407
            H   +  A K+ FG + F+ + +E           D +V MPTG GKSLC+QLP +++E  +TIV SPL +LI DQ+  L +L + A  L+  +   E D++++ L++    +K LY+TPE+   ++   ++ Q+L++   L+ F +DEAHCVSQWGHDFRP Y  LG LR+ + +V  +ALTATA+ +V+EDI  QL++      F     R NL ++I  K             A+ C+       +  K +   GIVYC +R++ + +A  + + G+   AYHAG+   +R +VQE W+  +   I+ AT +FGMG+DKP VRFV+H+ +P+++  +YQE+GRAGRDGL + C L+Y   DV  +R L+ +D       G  E   +   +Q   +  +CE    CR     + FG+
Sbjct:    3 HESAVHEALKKHFGHSKFKSDLQEKAVKCAVKKKQDVYVSMPTGSGKSLCFQLPGLMSENQITIVFSPLLALIKDQIDHLTKLKVPADSLNSKMSTKERDRVIMDLKAVRTNLKFLYITPEQAA-TKFFQDLLQTLHKHNKLAYFAVDEAHCVSQWGHDFRPDYLKLGELRSKYSDVIWLALTATASREVKEDIYKQLRLHQPVAQFSTPSFRKNLFYDIVYKNSIEDDFQHLADFARHCLGNPKEFKDTPKPQRGCGIVYCRTRDQVERMAIGVTKQGIGAVAYHAGLKTGERTEVQEAWMRGDQ-PIICATNSFGMGVDKPSVRFVIHWDVPQNVAAYYQESGRAGRDGLQSYCRLYYGREDVRSIRFLLQNDAHRARGRGDKELLTERAIKQFEKITEFCERTT-CRHKLFSDFFGD 419          
BLAST of ATP-dependent DNA helicase vs. Ensembl Fly
Match: RecQ4 (gene:FBgn0040290 transcript:FBtr0076729)

HSP 1 Score: 223.787 bits (569), Expect = 5.768e-59
Identity = 137/378 (36.24%), Postives = 205/378 (54.23%), Query Frame = 2
Query: 1247 VFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITE----GVTIVISPLKSLIHDQVTKLQE-LDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLL-GLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFN-RGNLRWEIRPKTAKKCISEIINLVNA-KFRD-QSGIVYCLSRNECDEVASVM------------------AQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWT--DVVRMRKLIYSDN 2293
            +FG   FR+ Q  AI  TLSG  + V + TG GKSLCYQLPA +       +T+VISPL SL+ DQVT +   L     H +    +    ++ +Q      +I +L V+PE VV   +       L Q   ++   IDEAHCVSQW H+FRP Y ++  +LR       ++ LTATAT   R  I+  L + +  +  I       NL   +     +   + ++ L+N+ +F   QS I+YC  R+EC+ +A  +                   +   + + YHAGM  ++R+ VQ+ +++ N  +IVVATIAFGMGI+KPD+R V+HY++P++ E + QE GRAGRDGLP+ C LF      D   +R+ +YS++
Sbjct:  865 MFGHTNFRKGQDRAIMRTLSGLSSLVTLSTGSGKSLCYQLPAYLYSRKVGAITLVISPLVSLMEDQVTGVPHFLRAHCLHTN----QTAPQRMKIQQMIANGEIDILLVSPEAVVAGERATGFGAILRQLPPIAFACIDEAHCVSQWSHNFRPSYLMICKVLRKNLGVRTVLGLTATATLPTRVSIINHLGISDGERGIISDIPLPDNLVLSVSKDENRD--AALLQLLNSERFEPCQSIIIYCTRRDECERIAGFIRTCVQDRREPTQDQTKKRKRVNWQAEPYHAGMPASRRRTVQKAFMS-NELRIVVATIAFGMGINKPDIRAVIHYNMPRNFESYVQEIGRAGRDGLPSHCHLFLDAKGGDQSELRRHVYSNS 1235          
BLAST of ATP-dependent DNA helicase vs. Ensembl Zebrafish
Match: blm (BLM RecQ like helicase [Source:ZFIN;Acc:ZDB-GENE-070702-5])

HSP 1 Score: 596.275 bits (1536), Expect = 0.000e+0
Identity = 309/683 (45.24%), Postives = 439/683 (64.28%), Query Frame = 2
Query: 1106 SYVPINEKQSKWAIEDFEYKSHPNDGETAEFSG-DFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEH-FDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVME-ISNVG--WGS-------TLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISKPRTTKDKDKVIGKEPVKEVDRLQKECYQKLVEVARKFANEAKIQNYANLFSNQTLMEMSERLPADLNDLKDITSFPQVKINKYGCNFLEVTKHYN 3118
            ++ P   K  K    +  Y++  +D     F G +FPH+  MM  F + FGL+ FR NQ EAIN++L G DTFV+MPTGGGKSLCYQLPA ++ GVT+VISPL+SLI DQV KL  LDI AT LSGD K++E  +I +QL  +   IKLLY TPEKV  S ++++  Q+L +R LL+R VIDEAHCVSQWGHDFRP Y+ L  LR  FPNVP++ALTATAT +V++DIL QL M    + F  SFNR NL++ + PK  KK   E I  +   +   SGIVYCLSRN+CD +A  + ++G+   AYHAG+SD+ R+ VQ  WIN++GC+++ ATIAFGMGIDKPDVR+V+H S+PKS+EG+YQE+GRAGRDG  + CVLFYS++DV+R+++LI  D  G  ++K  H   L  MV +CEN+ +CRR Q+L +FGEH F+   C      ICDNC  +  NK   R++TD  K + ++V +    VG  +G        TLN ++++F G+ +A+I         ++G G+ +SK++AER F  LV +  L E + +T +   VA++  GPK   +L+G   V+   ++  ++  K +    E V + + + K+C  +L ++ +K      I +Y N+FS  TL +++E L AD   L  I    + K+ KYG  F+E+ + Y+
Sbjct:  629 TFTPPAPKSIKTPNPEPLYRNPAHD----RFRGFNFPHSPEMMKIFHKKFGLHQFRFNQLEAINASLLGEDTFVLMPTGGGKSLCYQLPACVSAGVTVVISPLRSLIVDQVQKLTTLDICATSLSGDKKDSEAARIYMQLSRKDPAIKLLYATPEKVCASGRMISALQNLYERGLLARLVIDEAHCVSQWGHDFRPDYKRLHELRRMFPNVPIMALTATATPRVQKDILNQLAM-TRPQVFTMSFNRNNLKYSVLPKKPKKVDEECIQWIKKYYPRDSGIVYCLSRNDCDTLADSLQRAGIAALAYHAGLSDSDREYVQNKWINQDGCQVMCATIAFGMGIDKPDVRYVIHASLPKSVEGYYQESGRAGRDGEISHCVLFYSYSDVIRIKRLIAMDKDGNQQSKATHINNLHSMVHFCENVAECRRIQLLAYFGEHTFNTSFCKEHPEVICDNC--ARPNKYKSRNVTDDVKKIARFVQDNCEKVGNRYGKSAQQNRLTLNMLVEIFLGSKSARI------QTGMFGVGAAYSKHNAERLFKKLVLDNVLMEDLYITNSGQAVAYISAGPKAMSVLNGCMQVEFHETESASSIRKHRASVCEKVSKREEMVKKCLGELNDLCKKLGKVFGI-HYYNIFSTATLKKIAETLSADPEVLLQIDGVTEDKLEKYGAEFIELLQKYS 1297          
BLAST of ATP-dependent DNA helicase vs. Ensembl Zebrafish
Match: blm (BLM RecQ like helicase [Source:ZFIN;Acc:ZDB-GENE-070702-5])

HSP 1 Score: 596.275 bits (1536), Expect = 0.000e+0
Identity = 309/683 (45.24%), Postives = 439/683 (64.28%), Query Frame = 2
Query: 1106 SYVPINEKQSKWAIEDFEYKSHPNDGETAEFSG-DFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEH-FDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVME-ISNVG--WGS-------TLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISKPRTTKDKDKVIGKEPVKEVDRLQKECYQKLVEVARKFANEAKIQNYANLFSNQTLMEMSERLPADLNDLKDITSFPQVKINKYGCNFLEVTKHYN 3118
            ++ P   K  K    +  Y++  +D     F G +FPH+  MM  F + FGL+ FR NQ EAIN++L G DTFV+MPTGGGKSLCYQLPA ++ GVT+VISPL+SLI DQV KL  LDI AT LSGD K++E  +I +QL  +   IKLLY TPEKV  S ++++  Q+L +R LL+R VIDEAHCVSQWGHDFRP Y+ L  LR  FPNVP++ALTATAT +V++DIL QL M    + F  SFNR NL++ + PK  KK   E I  +   +   SGIVYCLSRN+CD +A  + ++G+   AYHAG+SD+ R+ VQ  WIN++GC+++ ATIAFGMGIDKPDVR+V+H S+PKS+EG+YQE+GRAGRDG  + CVLFYS++DV+R+++LI  D  G  ++K  H   L  MV +CEN+ +CRR Q+L +FGEH F+   C      ICDNC  +  NK   R++TD  K + ++V +    VG  +G        TLN ++++F G+ +A+I         ++G G+ +SK++AER F  LV +  L E + +T +   VA++  GPK   +L+G   V+   ++  ++  K +    E V + + + K+C  +L ++ +K      I +Y N+FS  TL +++E L AD   L  I    + K+ KYG  F+E+ + Y+
Sbjct:  599 TFTPPAPKSIKTPNPEPLYRNPAHD----RFRGFNFPHSPEMMKIFHKKFGLHQFRFNQLEAINASLLGEDTFVLMPTGGGKSLCYQLPACVSAGVTVVISPLRSLIVDQVQKLTTLDICATSLSGDKKDSEAARIYMQLSRKDPAIKLLYATPEKVCASGRMISALQNLYERGLLARLVIDEAHCVSQWGHDFRPDYKRLHELRRMFPNVPIMALTATATPRVQKDILNQLAM-TRPQVFTMSFNRNNLKYSVLPKKPKKVDEECIQWIKKYYPRDSGIVYCLSRNDCDTLADSLQRAGIAALAYHAGLSDSDREYVQNKWINQDGCQVMCATIAFGMGIDKPDVRYVIHASLPKSVEGYYQESGRAGRDGEISHCVLFYSYSDVIRIKRLIAMDKDGNQQSKATHINNLHSMVHFCENVAECRRIQLLAYFGEHTFNTSFCKEHPEVICDNC--ARPNKYKSRNVTDDVKKIARFVQDNCEKVGNRYGKSAQQNRLTLNMLVEIFLGSKSARI------QTGMFGVGAAYSKHNAERLFKKLVLDNVLMEDLYITNSGQAVAYISAGPKAMSVLNGCMQVEFHETESASSIRKHRASVCEKVSKREEMVKKCLGELNDLCKKLGKVFGI-HYYNIFSTATLKKIAETLSADPEVLLQIDGVTEDKLEKYGAEFIELLQKYS 1267          
BLAST of ATP-dependent DNA helicase vs. Ensembl Zebrafish
Match: blm (BLM RecQ like helicase [Source:ZFIN;Acc:ZDB-GENE-070702-5])

HSP 1 Score: 595.89 bits (1535), Expect = 0.000e+0
Identity = 309/683 (45.24%), Postives = 439/683 (64.28%), Query Frame = 2
Query: 1106 SYVPINEKQSKWAIEDFEYKSHPNDGETAEFSG-DFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEH-FDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVME-ISNVG--WGS-------TLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISKPRTTKDKDKVIGKEPVKEVDRLQKECYQKLVEVARKFANEAKIQNYANLFSNQTLMEMSERLPADLNDLKDITSFPQVKINKYGCNFLEVTKHYN 3118
            ++ P   K  K    +  Y++  +D     F G +FPH+  MM  F + FGL+ FR NQ EAIN++L G DTFV+MPTGGGKSLCYQLPA ++ GVT+VISPL+SLI DQV KL  LDI AT LSGD K++E  +I +QL  +   IKLLY TPEKV  S ++++  Q+L +R LL+R VIDEAHCVSQWGHDFRP Y+ L  LR  FPNVP++ALTATAT +V++DIL QL M    + F  SFNR NL++ + PK  KK   E I  +   +   SGIVYCLSRN+CD +A  + ++G+   AYHAG+SD+ R+ VQ  WIN++GC+++ ATIAFGMGIDKPDVR+V+H S+PKS+EG+YQE+GRAGRDG  + CVLFYS++DV+R+++LI  D  G  ++K  H   L  MV +CEN+ +CRR Q+L +FGEH F+   C      ICDNC  +  NK   R++TD  K + ++V +    VG  +G        TLN ++++F G+ +A+I         ++G G+ +SK++AER F  LV +  L E + +T +   VA++  GPK   +L+G   V+   ++  ++  K +    E V + + + K+C  +L ++ +K      I +Y N+FS  TL +++E L AD   L  I    + K+ KYG  F+E+ + Y+
Sbjct:  610 TFTPPAPKSIKTPNPEPLYRNPAHD----RFRGFNFPHSPEMMKIFHKKFGLHQFRFNQLEAINASLLGEDTFVLMPTGGGKSLCYQLPACVSAGVTVVISPLRSLIVDQVQKLTTLDICATSLSGDKKDSEAARIYMQLSRKDPAIKLLYATPEKVCASGRMISALQNLYERGLLARLVIDEAHCVSQWGHDFRPDYKRLHELRRMFPNVPIMALTATATPRVQKDILNQLAM-TRPQVFTMSFNRNNLKYSVLPKKPKKVDEECIQWIKKYYPRDSGIVYCLSRNDCDTLADSLQRAGIAALAYHAGLSDSDREYVQNKWINQDGCQVMCATIAFGMGIDKPDVRYVIHASLPKSVEGYYQESGRAGRDGEISHCVLFYSYSDVIRIKRLIAMDKDGNQQSKATHINNLHSMVHFCENVAECRRIQLLAYFGEHTFNTSFCKEHPEVICDNC--ARPNKYKSRNVTDDVKKIARFVQDNCEKVGNRYGKSAQQNRLTLNMLVEIFLGSKSARI------QTGMFGVGAAYSKHNAERLFKKLVLDNVLMEDLYITNSGQAVAYISAGPKAMSVLNGCMQVEFHETESASSIRKHRASVCEKVSKREEMVKKCLGELNDLCKKLGKVFGI-HYYNIFSTATLKKIAETLSADPEVLLQIDGVTEDKLEKYGAEFIELLQKYS 1278          
BLAST of ATP-dependent DNA helicase vs. Ensembl Zebrafish
Match: recql (RecQ helicase-like [Source:ZFIN;Acc:ZDB-GENE-050809-134])

HSP 1 Score: 381.333 bits (978), Expect = 3.318e-118
Identity = 213/578 (36.85%), Postives = 328/578 (56.75%), Query Frame = 2
Query: 1202 GDFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVMEISNVGWGSTLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISKPRTT-------------KDKDKVIGKEPVKEVDR 2896
             DF  ++ + +    +F L+ FR  Q+ AIN ++SG D F++MPTG GKSLCYQLPA+ ++G T+VI+PL SL+ DQ+  LQ +++ A  L+    + +  +IL  +  +    KLLYVTPEK+ +S+ LM+  +      LL+R  +DE HC SQWGHDFRP Y+LLG+L+  FPNVP+I LTATAT+ V +D    L ++         FNR NL +E+R K  + C  +I +L+  ++++QSGIVY  S+ + + VA+ + +  +  Q YHA M  + +  V + W +    ++VVAT+AFGMGIDK DVRFV+H++I KSIE +YQE+GRAGRD  PA C++F+ + D+ R+  ++  +N+G        +Q+L  MV+YC+N+  CRR  +  HF E ++ + C      +CD C++ ND   +  D+T HA+ V+  V   S++    T   V D + G   AK          +  T    S+ + E    HL+  GY  E    T       ++  G K  LL +   ++ M + +  T+             K + KV GK P +  D+
Sbjct:   77 SDFSWSKEVQVNLCNIFQLSKFRPLQRAAINLSMSGKDLFLVMPTGRGKSLCYQLPALCSKGFTLVIAPLVSLMEDQLMYLQSVNVPAVTLNASSSKEDSKRILAGMTDKNSPFKLLYVTPEKIAKSKLLMSKLEKAFNMGLLARIAVDEVHCCSQWGHDFRPDYKLLGILKRQFPNVPLIGLTATATSNVLKDCQKILCVQEPVT-LTAPFNRPNLYYEVRFKDNEDCTDQIASLIRGRYKNQSGIVYVFSQKDAEVVATELQKRDIVAQPYHANMEPSHKSLVHQRW-SSKKIQVVVATVAFGMGIDKADVRFVIHHTISKSIENYYQESGRAGRDDSPADCIVFFGFMDIFRISTMVVMENTG--------QQKLHNMVAYCQNVDRCRRAMMAIHFDEVWNDEECNE----MCDVCRHGND--YITMDITQHARDVLHIVELASSMDEKLTPLKVCDAWLGKGPAK------QRKMIKLTS--LSRLEVESVIIHLLLHGYFSEDFSFTPYT-THFYLKLGRKAALLKNSGHSITMKMRRRGTSQSTVKKVSESSGEKSRSKVEGKRPAESGDQ 629          
BLAST of ATP-dependent DNA helicase vs. Ensembl Zebrafish
Match: recql (RecQ helicase-like [Source:ZFIN;Acc:ZDB-GENE-050809-134])

HSP 1 Score: 381.333 bits (978), Expect = 3.318e-118
Identity = 213/578 (36.85%), Postives = 328/578 (56.75%), Query Frame = 2
Query: 1202 GDFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVMEISNVGWGSTLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISKPRTT-------------KDKDKVIGKEPVKEVDR 2896
             DF  ++ + +    +F L+ FR  Q+ AIN ++SG D F++MPTG GKSLCYQLPA+ ++G T+VI+PL SL+ DQ+  LQ +++ A  L+    + +  +IL  +  +    KLLYVTPEK+ +S+ LM+  +      LL+R  +DE HC SQWGHDFRP Y+LLG+L+  FPNVP+I LTATAT+ V +D    L ++         FNR NL +E+R K  + C  +I +L+  ++++QSGIVY  S+ + + VA+ + +  +  Q YHA M  + +  V + W +    ++VVAT+AFGMGIDK DVRFV+H++I KSIE +YQE+GRAGRD  PA C++F+ + D+ R+  ++  +N+G        +Q+L  MV+YC+N+  CRR  +  HF E ++ + C      +CD C++ ND   +  D+T HA+ V+  V   S++    T   V D + G   AK          +  T    S+ + E    HL+  GY  E    T       ++  G K  LL +   ++ M + +  T+             K + KV GK P +  D+
Sbjct:   77 SDFSWSKEVQVNLCNIFQLSKFRPLQRAAINLSMSGKDLFLVMPTGRGKSLCYQLPALCSKGFTLVIAPLVSLMEDQLMYLQSVNVPAVTLNASSSKEDSKRILAGMTDKNSPFKLLYVTPEKIAKSKLLMSKLEKAFNMGLLARIAVDEVHCCSQWGHDFRPDYKLLGILKRQFPNVPLIGLTATATSNVLKDCQKILCVQEPVT-LTAPFNRPNLYYEVRFKDNEDCTDQIASLIRGRYKNQSGIVYVFSQKDAEVVATELQKRDIVAQPYHANMEPSHKSLVHQRW-SSKKIQVVVATVAFGMGIDKADVRFVIHHTISKSIENYYQESGRAGRDDSPADCIVFFGFMDIFRISTMVVMENTG--------QQKLHNMVAYCQNVDRCRRAMMAIHFDEVWNDEECNE----MCDVCRHGND--YITMDITQHARDVLHIVELASSMDEKLTPLKVCDAWLGKGPAK------QRKMIKLTS--LSRLEVESVIIHLLLHGYFSEDFSFTPYT-THFYLKLGRKAALLKNSGHSITMKMRRRGTSQSTVKKVSESSGEKSRSKVEGKRPAESGDQ 629          
BLAST of ATP-dependent DNA helicase vs. Ensembl Xenopus
Match: blm (Bloom syndrome RecQ like helicase [Source:NCBI gene;Acc:100124311])

HSP 1 Score: 577.785 bits (1488), Expect = 0.000e+0
Identity = 305/707 (43.14%), Postives = 442/707 (62.52%), Query Frame = 2
Query: 1199 SGDFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGE-HFDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVMEISNVGWGS--------TLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISKPRTTKDKDKVIGKEPVKEVDRLQKECYQKLVEVARKFANEAKIQNYANLFSNQTLMEMSERLPADLNDLKDITSFPQVKINKYGCNFLEVTKHYNLLLTKHKQEMKDLEKKLTV-------------DIKPENAKSSYFSSTSKAPAKGYNRK 3253
            S +FPH++ MM  F + FGL+ FR NQ EAIN+ L G D F++MPTGGGKSLCYQLP  ++ GVTIVISPL+SLI DQV KL  LDI AT+L+GD  +AE   I LQL  +   IKLLYVTPEKV  S +L++  ++L +R+LL+RFVIDEAHCVSQWGHDFRP Y+ L +LR  F +VPM+ALTATA  +V++DIL QLKM    + F  SFNR NL++++ PK  K+   + +  +     + SGI+YCLSR+ECD +A  + + GL   AYHAG++D+ R  VQ  WIN++ C+++ ATIAFGMGIDKPDVR+V+H S+PKS+EG+YQE+GRAGRDG  + C+LFYS++DV R+R+LI  +  G   TK+ H   L+ MV YCEN+++CRR Q+L +FGE +F+   C       CDNC    + K   R++TD  + +V++V E  +   G         TLN ++D+F G+ +AKI         L+G G+ +S+++AER F  LV +  L E++ +T  D  VA+V  G + Q +L+G  TV+   ++  ++  K K        + + + K+C  +L E+ ++      + +Y N+F+  T+  ++E L  + + L  I    + K+ KYG   ++V + Y    ++    ++D+ +K +              D      KSSYFS+ S    KG  RK
Sbjct:  600 SFNFPHSKEMMKIFHKKFGLHRFRTNQLEAINACLLGEDCFILMPTGGGKSLCYQLPGCVSPGVTIVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDAEAASIYLQLSKKDPIIKLLYVTPEKVCASTRLISTMENLYERQLLARFVIDEAHCVSQWGHDFRPDYKRLNMLRQKFQSVPMMALTATANPRVQKDILNQLKM-TKPQIFTMSFNRDNLKYDVLPKKPKRVALDCVEWIKKYHPNDSGIIYCLSRHECDTMADTLQKGGLAALAYHAGLADSNRDYVQHKWINQDDCQVICATIAFGMGIDKPDVRYVIHASLPKSVEGYYQESGRAGRDGEISHCLLFYSYSDVTRIRRLIQMEKDGNSHTKQTHFNNLYSMVHYCENVVECRRMQLLAYFGENNFNPNFCKENTRVSCDNCLGRKEFK--SRNVTDDVRNIVRFVQENCSSARGKGRSNSSRLTLNMMVDIFLGSKSAKI------QTGLFGKGAAYSRHNAERLFRKLVLDRILDEELYITANDQAVAYVKTGERAQAVLNGFLTVEFQDTENASSLRKQKASVVANTSQREEMVKKCQAELTELCKRLGKIFGV-HYFNIFNTATIRRIAESLSPEPDVLLQIDGVTEDKLEKYGAELIDVLQKY----SEWTLPVEDIHQKSSGPANFSARRYDSDDDDGERGEKSSYFSTKSN---KGTKRK 1289          
BLAST of ATP-dependent DNA helicase vs. Ensembl Xenopus
Match: blm (Bloom syndrome RecQ like helicase [Source:NCBI gene;Acc:100124311])

HSP 1 Score: 575.474 bits (1482), Expect = 0.000e+0
Identity = 291/649 (44.84%), Postives = 421/649 (64.87%), Query Frame = 2
Query: 1199 SGDFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGE-HFDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVMEISNVGWGS--------TLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISKPRTTKDKDKVIGKEPVKEVDRLQKECYQKLVEVARKFANEAKIQNYANLFSNQTLMEMSERLPADLNDLKDITSFPQVKINKYGCNFLEVTKHYN 3118
            S +FPH++ MM  F + FGL+ FR NQ EAIN+ L G D F++MPTGGGKSLCYQLP  ++ GVTIVISPL+SLI DQV KL  LDI AT+L+GD  +AE   I LQL  +   IKLLYVTPEKV  S +L++  ++L +R+LL+RFVIDEAHCVSQWGHDFRP Y+ L +LR  F +VPM+ALTATA  +V++DIL QLKM    + F  SFNR NL++++ PK  K+   + +  +     + SGI+YCLSR+ECD +A  + + GL   AYHAG++D+ R  VQ  WIN++ C+++ ATIAFGMGIDKPDVR+V+H S+PKS+EG+YQE+GRAGRDG  + C+LFYS++DV R+R+LI  +  G   TK+ H   L+ MV YCEN+++CRR Q+L +FGE +F+   C       CDNC    + K   R++TD  + +V++V E  +   G         TLN ++D+F G+ +AKI         L+G G+ +S+++AER F  LV +  L E++ +T  D  VA+V  G + Q +L+G  TV+   ++  ++  K K        + + + K+C  +L E+ ++      + +Y N+F+  T+  ++E L  + + L  I    + K+ KYG   ++V + Y+
Sbjct:  532 SFNFPHSKEMMKIFHKKFGLHRFRTNQLEAINACLLGEDCFILMPTGGGKSLCYQLPGCVSPGVTIVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDAEAASIYLQLSKKDPIIKLLYVTPEKVCASTRLISTMENLYERQLLARFVIDEAHCVSQWGHDFRPDYKRLNMLRQKFQSVPMMALTATANPRVQKDILNQLKM-TKPQIFTMSFNRDNLKYDVLPKKPKRVALDCVEWIKKYHPNDSGIIYCLSRHECDTMADTLQKGGLAALAYHAGLADSNRDYVQHKWINQDDCQVICATIAFGMGIDKPDVRYVIHASLPKSVEGYYQESGRAGRDGEISHCLLFYSYSDVTRIRRLIQMEKDGNSHTKQTHFNNLYSMVHYCENVVECRRMQLLAYFGENNFNPNFCKENTRVSCDNCLGRKEFK--SRNVTDDVRNIVRFVQENCSSARGKGRSNSSRLTLNMMVDIFLGSKSAKI------QTGLFGKGAAYSRHNAERLFRKLVLDRILDEELYITANDQAVAYVKTGERAQAVLNGFLTVEFQDTENASSLRKQKASVVANTSQREEMVKKCQAELTELCKRLGKIFGV-HYFNIFNTATIRRIAESLSPEPDVLLQIDGVTEDKLEKYGAELIDVLQKYS 1170          
BLAST of ATP-dependent DNA helicase vs. Ensembl Xenopus
Match: blm (Bloom syndrome RecQ like helicase [Source:NCBI gene;Acc:100124311])

HSP 1 Score: 574.704 bits (1480), Expect = 0.000e+0
Identity = 305/707 (43.14%), Postives = 442/707 (62.52%), Query Frame = 2
Query: 1199 SGDFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGE-HFDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVMEISNVGWGS--------TLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISKPRTTKDKDKVIGKEPVKEVDRLQKECYQKLVEVARKFANEAKIQNYANLFSNQTLMEMSERLPADLNDLKDITSFPQVKINKYGCNFLEVTKHYNLLLTKHKQEMKDLEKKLTV-------------DIKPENAKSSYFSSTSKAPAKGYNRK 3253
            S +FPH++ MM  F + FGL+ FR NQ EAIN+ L G D F++MPTGGGKSLCYQLP  ++ GVTIVISPL+SLI DQV KL  LDI AT+L+GD  +AE   I LQL  +   IKLLYVTPEKV  S +L++  ++L +R+LL+RFVIDEAHCVSQWGHDFRP Y+ L +LR  F +VPM+ALTATA  +V++DIL QLKM    + F  SFNR NL++++ PK  K+   + +  +     + SGI+YCLSR+ECD +A  + + GL   AYHAG++D+ R  VQ  WIN++ C+++ ATIAFGMGIDKPDVR+V+H S+PKS+EG+YQE+GRAGRDG  + C+LFYS++DV R+R+LI  +  G   TK+ H   L+ MV YCEN+++CRR Q+L +FGE +F+   C       CDNC    + K   R++TD  + +V++V E  +   G         TLN ++D+F G+ +AKI         L+G G+ +S+++AER F  LV +  L E++ +T  D  VA+V  G + Q +L+G  TV+   ++  ++  K K        + + + K+C  +L E+ ++      + +Y N+F+  T+  ++E L  + + L  I    + K+ KYG   ++V + Y    ++    ++D+ +K +              D      KSSYFS+ S    KG  RK
Sbjct:  379 SFNFPHSKEMMKIFHKKFGLHRFRTNQLEAINACLLGEDCFILMPTGGGKSLCYQLPGCVSPGVTIVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDAEAASIYLQLSKKDPIIKLLYVTPEKVCASTRLISTMENLYERQLLARFVIDEAHCVSQWGHDFRPDYKRLNMLRQKFQSVPMMALTATANPRVQKDILNQLKM-TKPQIFTMSFNRDNLKYDVLPKKPKRVALDCVEWIKKYHPNDSGIIYCLSRHECDTMADTLQKGGLAALAYHAGLADSNRDYVQHKWINQDDCQVICATIAFGMGIDKPDVRYVIHASLPKSVEGYYQESGRAGRDGEISHCLLFYSYSDVTRIRRLIQMEKDGNSHTKQTHFNNLYSMVHYCENVVECRRMQLLAYFGENNFNPNFCKENTRVSCDNCLGRKEFK--SRNVTDDVRNIVRFVQENCSSARGKGRSNSSRLTLNMMVDIFLGSKSAKI------QTGLFGKGAAYSRHNAERLFRKLVLDRILDEELYITANDQAVAYVKTGERAQAVLNGFLTVEFQDTENASSLRKQKASVVANTSQREEMVKKCQAELTELCKRLGKIFGV-HYFNIFNTATIRRIAESLSPEPDVLLQIDGVTEDKLEKYGAELIDVLQKY----SEWTLPVEDIHQKSSGPANFSARRYDSDDDDGERGEKSSYFSTKSN---KGTKRK 1068          
BLAST of ATP-dependent DNA helicase vs. Ensembl Xenopus
Match: blm (Bloom syndrome RecQ like helicase [Source:NCBI gene;Acc:100124311])

HSP 1 Score: 521.931 bits (1343), Expect = 2.441e-163
Identity = 283/707 (40.03%), Postives = 417/707 (58.98%), Query Frame = 2
Query: 1199 SGDFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGE-HFDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVMEISNVGWGS--------TLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISKPRTTKDKDKVIGKEPVKEVDRLQKECYQKLVEVARKFANEAKIQNYANLFSNQTLMEMSERLPADLNDLKDITSFPQVKINKYGCNFLEVTKHYNLLLTKHKQEMKDLEKKLTV-------------DIKPENAKSSYFSSTSKAPAKGYNRK 3253
            S +FPH++ MM  F + FGL+ FR NQ EAIN+ L G D F++MPTGGGKSLCYQLP  ++ GVTIVISPL+SLI DQV KL  LD+ A                                      S +L++  ++L +R+LL+RFVIDEAHCVSQWGHDFRP Y+ L +LR  F +VPM+ALTATA  +V++DIL QLKM    + F  SFNR NL++++ PK  K+   + +  +     + SGI+YCLSR+ECD +A  + + GL   AYHAG++D+ R  VQ  WIN++ C+++ ATIAFGMGIDKPDVR+V+H S+PKS+EG+YQE+GRAGRDG  + C+LFYS++DV R+R+LI  +  G   TK+ H   L+ MV YCEN+++CRR Q+L +FGE +F+   C       CDNC    + K   R++TD  + +V++V E  +   G         TLN ++D+F G+ +AKI         L+G G+ +S+++AER F  LV +  L E++ +T  D  VA+V  G + Q +L+G  TV+   ++  ++  K K        + + + K+C  +L E+ ++      + +Y N+F+  T+  ++E L  + + L  I    + K+ KYG   ++V + Y    ++    ++D+ +K +              D      KSSYFS+ S    KG  RK
Sbjct:  600 SFNFPHSKEMMKIFHKKFGLHRFRTNQLEAINACLLGEDCFILMPTGGGKSLCYQLPGCVSPGVTIVISPLRSLIVDQVQKLTSLDVCA--------------------------------------STRLISTMENLYERQLLARFVIDEAHCVSQWGHDFRPDYKRLNMLRQKFQSVPMMALTATANPRVQKDILNQLKM-TKPQIFTMSFNRDNLKYDVLPKKPKRVALDCVEWIKKYHPNDSGIIYCLSRHECDTMADTLQKGGLAALAYHAGLADSNRDYVQHKWINQDDCQVICATIAFGMGIDKPDVRYVIHASLPKSVEGYYQESGRAGRDGEISHCLLFYSYSDVTRIRRLIQMEKDGNSHTKQTHFNNLYSMVHYCENVVECRRMQLLAYFGENNFNPNFCKENTRVSCDNCLGRKEFK--SRNVTDDVRNIVRFVQENCSSARGKGRSNSSRLTLNMMVDIFLGSKSAKI------QTGLFGKGAAYSRHNAERLFRKLVLDRILDEELYITANDQAVAYVKTGERAQAVLNGFLTVEFQDTENASSLRKQKASVVANTSQREEMVKKCQAELTELCKRLGKIFGV-HYFNIFNTATIRRIAESLSPEPDVLLQIDGVTEDKLEKYGAELIDVLQKY----SEWTLPVEDIHQKSSGPANFSARRYDSDDDDGERGEKSSYFSTKSN---KGTKRK 1251          
BLAST of ATP-dependent DNA helicase vs. Ensembl Xenopus
Match: blm (Bloom syndrome RecQ like helicase [Source:NCBI gene;Acc:100124311])

HSP 1 Score: 506.138 bits (1302), Expect = 3.071e-161
Identity = 244/473 (51.59%), Postives = 330/473 (69.77%), Query Frame = 2
Query: 1199 SGDFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGE-HFDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVMEISNVGWGS--------TLNYVLDVF 2590
            S +FPH++ MM  F + FGL+ FR NQ EAIN+ L G D F++MPTGGGKSLCYQLP  ++ GVTIVISPL+SLI DQV KL  LDI AT+L+GD  +AE   I LQL  +   IKLLYVTPEKV  S +L++  ++L +R+LL+RFVIDEAHCVSQWGHDFRP Y+ L +LR  F +VPM+ALTATA  +V++DIL QLKM    + F  SFNR NL++++ PK  K+   + +  +     + SGI+YCLSR+ECD +A  + + GL   AYHAG++D+ R  VQ  WIN++ C+++ ATIAFGMGIDKPDVR+V+H S+PKS+EG+YQE+GRAGRDG  + C+LFYS++DV R+R+LI  +  G   TK+ H   L+ MV YCEN+++CRR Q+L +FGE +F+   C       CDNC    + K   R++TD  + +V++V E  +   G         TLN ++D+F
Sbjct:  379 SFNFPHSKEMMKIFHKKFGLHRFRTNQLEAINACLLGEDCFILMPTGGGKSLCYQLPGCVSPGVTIVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDAEAASIYLQLSKKDPIIKLLYVTPEKVCASTRLISTMENLYERQLLARFVIDEAHCVSQWGHDFRPDYKRLNMLRQKFQSVPMMALTATANPRVQKDILNQLKM-TKPQIFTMSFNRDNLKYDVLPKKPKRVALDCVEWIKKYHPNDSGIIYCLSRHECDTMADTLQKGGLAALAYHAGLADSNRDYVQHKWINQDDCQVICATIAFGMGIDKPDVRYVIHASLPKSVEGYYQESGRAGRDGEISHCLLFYSYSDVTRIRRLIQMEKDGNSHTKQTHFNNLYSMVHYCENVVECRRMQLLAYFGENNFNPNFCKENTRVSCDNCLGRKEFK--SRNVTDDVRNIVRFVQENCSSARGKGRSNSSRLTLNMMVDIF 848          
BLAST of ATP-dependent DNA helicase vs. Ensembl Mouse
Match: Blm (Bloom syndrome, RecQ like helicase [Source:MGI Symbol;Acc:MGI:1328362])

HSP 1 Score: 574.704 bits (1480), Expect = 0.000e+0
Identity = 295/648 (45.52%), Postives = 418/648 (64.51%), Query Frame = 2
Query: 1205 DFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEH-FDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVMEIS------NVGWGS--TLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQ-MPISKPRTTKDKDKVIGKEPVKEVDRLQKECYQKLVEVARKFANEAKIQNYANLFSNQTLMEMSERLPADLNDLKDITSFPQVKINKYGCNFLEVTKHYN 3118
            +FPHT+ MM  F + FGL+ FR NQ EAIN+ L G D F++MPTGGGKSLCYQLPA ++ GVTIVISPL+SLI DQV KL   DI AT+L+GD  ++E   I LQL  +   IKLLYVTPEKV  S +L++  ++L +RKLL+RFVIDEAHCVSQWGHDFR  Y+ + +LR  FP+VP++ALTATA  +V++DIL QLK+    + F  SFNR NL++ + PK  KK   + +  +       SGI+YCLSR ECD +A  + + GL   AYHAG+SD+ R +VQ  WIN++ C+++ ATIAFGMGIDKPDVRFV+H S+PKS+EG+YQE+GRAGRDG  + CVLFY++ DV R+++LI  +  G   TK+ H   L+ MV YCEN+ +CRR Q+L +FGE  F+   C       CDNC  + D K   +D+TD  K ++++V E S      N+G     TLN ++D+F G+ +AK+  K G    ++G G+ +S+++AER F  L+ +  L E + +   D  +A+V+ G K   +LSG+  V  M      + K +  ++ K  V + + + K+C  +L EV +       + +Y N+F+  TL +++E L +D   L  I    + K+ KYG   + V + Y+
Sbjct:  659 NFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTIVISPLRSLIVDQVQKLTSFDIPATYLTGDKTDSEAANIYLQLSKKDPIIKLLYVTPEKVCASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKIL-RPQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQREGLAALAYHAGLSDSARDEVQHKWINQDNCQVICATIAFGMGIDKPDVRFVIHASLPKSMEGYYQESGRAGRDGEISHCVLFYTYHDVTRLKRLIMMEKDGNYHTKETHVNNLYSMVHYCENITECRRIQLLAYFGEKGFNPDFCKKYPDVSCDNCCKTKDYKT--KDVTDDVKNIIRFVQEHSSSPGTRNIGPAGRFTLNMLVDIFLGSKSAKV--KSG----IFGKGTTYSRHNAERLFKKLILDKILDEDLYINANDQPIAYVMLGTKAHSVLSGHLKVDFMETENSSSIKKQKALVAK--VSQREEVVKKCLGELTEVCKLLGKVFGV-HYFNIFNTATLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVIPVLQKYS 1294          
BLAST of ATP-dependent DNA helicase vs. Ensembl Mouse
Match: Blm (Bloom syndrome, RecQ like helicase [Source:MGI Symbol;Acc:MGI:1328362])

HSP 1 Score: 574.318 bits (1479), Expect = 0.000e+0
Identity = 295/648 (45.52%), Postives = 418/648 (64.51%), Query Frame = 2
Query: 1205 DFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEH-FDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVMEIS------NVGWGS--TLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQ-MPISKPRTTKDKDKVIGKEPVKEVDRLQKECYQKLVEVARKFANEAKIQNYANLFSNQTLMEMSERLPADLNDLKDITSFPQVKINKYGCNFLEVTKHYN 3118
            +FPHT+ MM  F + FGL+ FR NQ EAIN+ L G D F++MPTGGGKSLCYQLPA ++ GVTIVISPL+SLI DQV KL   DI AT+L+GD  ++E   I LQL  +   IKLLYVTPEKV  S +L++  ++L +RKLL+RFVIDEAHCVSQWGHDFR  Y+ + +LR  FP+VP++ALTATA  +V++DIL QLK+    + F  SFNR NL++ + PK  KK   + +  +       SGI+YCLSR ECD +A  + + GL   AYHAG+SD+ R +VQ  WIN++ C+++ ATIAFGMGIDKPDVRFV+H S+PKS+EG+YQE+GRAGRDG  + CVLFY++ DV R+++LI  +  G   TK+ H   L+ MV YCEN+ +CRR Q+L +FGE  F+   C       CDNC  + D K   +D+TD  K ++++V E S      N+G     TLN ++D+F G+ +AK+  K G    ++G G+ +S+++AER F  L+ +  L E + +   D  +A+V+ G K   +LSG+  V  M      + K +  ++ K  V + + + K+C  +L EV +       + +Y N+F+  TL +++E L +D   L  I    + K+ KYG   + V + Y+
Sbjct:  656 NFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTIVISPLRSLIVDQVQKLTSFDIPATYLTGDKTDSEAANIYLQLSKKDPIIKLLYVTPEKVCASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKIL-RPQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQREGLAALAYHAGLSDSARDEVQHKWINQDNCQVICATIAFGMGIDKPDVRFVIHASLPKSMEGYYQESGRAGRDGEISHCVLFYTYHDVTRLKRLIMMEKDGNYHTKETHVNNLYSMVHYCENITECRRIQLLAYFGEKGFNPDFCKKYPDVSCDNCCKTKDYKT--KDVTDDVKNIIRFVQEHSSSPGTRNIGPAGRFTLNMLVDIFLGSKSAKV--KSG----IFGKGTTYSRHNAERLFKKLILDKILDEDLYINANDQPIAYVMLGTKAHSVLSGHLKVDFMETENSSSIKKQKALVAK--VSQREEVVKKCLGELTEVCKLLGKVFGV-HYFNIFNTATLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVIPVLQKYS 1291          
BLAST of ATP-dependent DNA helicase vs. Ensembl Mouse
Match: Recql (RecQ protein-like [Source:MGI Symbol;Acc:MGI:103021])

HSP 1 Score: 381.333 bits (978), Expect = 3.063e-118
Identity = 204/565 (36.11%), Postives = 321/565 (56.81%), Query Frame = 2
Query: 1205 DFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIR--PKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVMEISNVGWGSTLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISKPRTTKDKDKVIGKEPVKEVD 2893
            DFP   ++    + VF L  FR  Q E IN T++  D F++MPTGGGKSLCYQLPA+ ++G T+VI PL SL+ DQ+  L++L I AT L+    +  V  +  ++ ++  Q+KL+YVTPEK+ +S+  M+  +   +   L+   +DE HC SQWGHDFRP Y+ LG+L+  FPN  ++ LTATAT  V +D+  ++        F  SFNR NL +E+R  P +A+    +I+ L+N +++ QSGI+YC S+ + +++   + + G+    YHA M    + +V   W + N  ++VVAT+AFGMGIDKPDVRFV+H+S+ KS+E +YQE+GRAGRD   A C+L+Y + D+ R+  ++  +N G        +Q+L+ MVSYC+N+  CRR  +  HF E ++   C      +CDNC    D     +++T H + ++K + +   +    T   ++D + G   AK+    G             + D ER   H + + YL E    T A   ++++  GP+  LL +    V M + K   +  +  +     V++VD
Sbjct:   72 DFPWFGKVKDVLQNVFKLQKFRPLQLETINVTMARKDIFLVMPTGGGKSLCYQLPALCSDGFTLVICPLISLMEDQLMVLKQLGISATMLNASSSKEHVKWVHAEMVNKNSQLKLIYVTPEKIAKSKMFMSRLEKAYEAGRLTGAAVDEVHCCSQWGHDFRPDYKALGILKRQFPNASLMGLTATATNHVLKDV-QKILCVGKCLTFTASFNRPNLFYEVRQKPSSAEDFTEDIVKLINGRYKGQSGIIYCFSQKDSEQITISLQKLGIHAGTYHANMEPEDKTKVHTQW-SANELQVVVATVAFGMGIDKPDVRFVIHHSMSKSMENYYQESGRAGRDDSRADCILYYGFGDIFRISSMVVMENVG--------QQKLYEMVSYCQNVSKCRRVLIAQHFDEVWNADACNK----MCDNC--CKDVSFEKKNVTQHCRDLIKILKQAEGLNEKLTPLKLIDAWMGKGAAKL-RVAGV------VAPALPREDLERIVAHALLQQYLKEDYSFT-AYATISYLKVGPRACLLSNEAHAVTMQVKKSAQSSVRGALSEARQVEQVD 612          
BLAST of ATP-dependent DNA helicase vs. Ensembl Mouse
Match: Recql (RecQ protein-like [Source:MGI Symbol;Acc:MGI:103021])

HSP 1 Score: 381.333 bits (978), Expect = 3.407e-118
Identity = 204/565 (36.11%), Postives = 321/565 (56.81%), Query Frame = 2
Query: 1205 DFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIR--PKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVMEISNVGWGSTLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISKPRTTKDKDKVIGKEPVKEVD 2893
            DFP   ++    + VF L  FR  Q E IN T++  D F++MPTGGGKSLCYQLPA+ ++G T+VI PL SL+ DQ+  L++L I AT L+    +  V  +  ++ ++  Q+KL+YVTPEK+ +S+  M+  +   +   L+   +DE HC SQWGHDFRP Y+ LG+L+  FPN  ++ LTATAT  V +D+  ++        F  SFNR NL +E+R  P +A+    +I+ L+N +++ QSGI+YC S+ + +++   + + G+    YHA M    + +V   W + N  ++VVAT+AFGMGIDKPDVRFV+H+S+ KS+E +YQE+GRAGRD   A C+L+Y + D+ R+  ++  +N G        +Q+L+ MVSYC+N+  CRR  +  HF E ++   C      +CDNC    D     +++T H + ++K + +   +    T   ++D + G   AK+    G             + D ER   H + + YL E    T A   ++++  GP+  LL +    V M + K   +  +  +     V++VD
Sbjct:   72 DFPWFGKVKDVLQNVFKLQKFRPLQLETINVTMARKDIFLVMPTGGGKSLCYQLPALCSDGFTLVICPLISLMEDQLMVLKQLGISATMLNASSSKEHVKWVHAEMVNKNSQLKLIYVTPEKIAKSKMFMSRLEKAYEAGRLTGAAVDEVHCCSQWGHDFRPDYKALGILKRQFPNASLMGLTATATNHVLKDV-QKILCVGKCLTFTASFNRPNLFYEVRQKPSSAEDFTEDIVKLINGRYKGQSGIIYCFSQKDSEQITISLQKLGIHAGTYHANMEPEDKTKVHTQW-SANELQVVVATVAFGMGIDKPDVRFVIHHSMSKSMENYYQESGRAGRDDSRADCILYYGFGDIFRISSMVVMENVG--------QQKLYEMVSYCQNVSKCRRVLIAQHFDEVWNADACNK----MCDNC--CKDVSFEKKNVTQHCRDLIKILKQAEGLNEKLTPLKLIDAWMGKGAAKL-RVAGV------VAPALPREDLERIVAHALLQQYLKEDYSFT-AYATISYLKVGPRACLLSNEAHAVTMQVKKSAQSSVRGALSEARQVEQVD 612          
BLAST of ATP-dependent DNA helicase vs. Ensembl Mouse
Match: Recql (RecQ protein-like [Source:MGI Symbol;Acc:MGI:103021])

HSP 1 Score: 381.719 bits (979), Expect = 3.838e-118
Identity = 204/565 (36.11%), Postives = 321/565 (56.81%), Query Frame = 2
Query: 1205 DFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIR--PKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVMEISNVGWGSTLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISKPRTTKDKDKVIGKEPVKEVD 2893
            DFP   ++    + VF L  FR  Q E IN T++  D F++MPTGGGKSLCYQLPA+ ++G T+VI PL SL+ DQ+  L++L I AT L+    +  V  +  ++ ++  Q+KL+YVTPEK+ +S+  M+  +   +   L+   +DE HC SQWGHDFRP Y+ LG+L+  FPN  ++ LTATAT  V +D+  ++        F  SFNR NL +E+R  P +A+    +I+ L+N +++ QSGI+YC S+ + +++   + + G+    YHA M    + +V   W + N  ++VVAT+AFGMGIDKPDVRFV+H+S+ KS+E +YQE+GRAGRD   A C+L+Y + D+ R+  ++  +N G        +Q+L+ MVSYC+N+  CRR  +  HF E ++   C      +CDNC    D     +++T H + ++K + +   +    T   ++D + G   AK+    G             + D ER   H + + YL E    T A   ++++  GP+  LL +    V M + K   +  +  +     V++VD
Sbjct:   72 DFPWFGKVKDVLQNVFKLQKFRPLQLETINVTMARKDIFLVMPTGGGKSLCYQLPALCSDGFTLVICPLISLMEDQLMVLKQLGISATMLNASSSKEHVKWVHAEMVNKNSQLKLIYVTPEKIAKSKMFMSRLEKAYEAGRLTGAAVDEVHCCSQWGHDFRPDYKALGILKRQFPNASLMGLTATATNHVLKDV-QKILCVGKCLTFTASFNRPNLFYEVRQKPSSAEDFTEDIVKLINGRYKGQSGIIYCFSQKDSEQITISLQKLGIHAGTYHANMEPEDKTKVHTQW-SANELQVVVATVAFGMGIDKPDVRFVIHHSMSKSMENYYQESGRAGRDDSRADCILYYGFGDIFRISSMVVMENVG--------QQKLYEMVSYCQNVSKCRRVLIAQHFDEVWNADACNK----MCDNC--CKDVSFEKKNVTQHCRDLIKILKQAEGLNEKLTPLKLIDAWMGKGAAKL-RVAGV------VAPALPREDLERIVAHALLQQYLKEDYSFT-AYATISYLKVGPRACLLSNEAHAVTMQVKKSAQSSVRGALSEARQVEQVD 612          
BLAST of ATP-dependent DNA helicase vs. UniProt/SwissProt
Match: sp|P54132|BLM_HUMAN (Bloom syndrome protein OS=Homo sapiens OX=9606 GN=BLM PE=1 SV=1)

HSP 1 Score: 583.178 bits (1502), Expect = 0.000e+0
Identity = 295/650 (45.38%), Postives = 422/650 (64.92%), Query Frame = 2
Query: 1208 FPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEH-FDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVME---------ISNVGWGS--TLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQ-MPISKPRTTKDKDKVIGKEPVKEVDRLQKECYQKLVEVARKFANEAKIQNYANLFSNQTLMEMSERLPADLNDLKDITSFPQVKINKYGCNFLEVTKHYN 3118
            FPHT+ MM  F + FGL+ FR NQ EAIN+ L G D F++MPTGGGKSLCYQLPA ++ GVT+VISPL+SLI DQV KL  LDI AT+L+GD  ++E   I LQL  +   IKLLYVTPEK+  S +L++  ++L +RKLL+RFVIDEAHCVSQWGHDFR  Y+ + +LR  FP+VP++ALTATA  +V++DIL QLK+    + F  SFNR NL++ + PK  KK   + +  +       SGI+YCLSR ECD +A  + + GL   AYHAG+SD+ R +VQ+ WIN++GC+++ ATIAFGMGIDKPDVRFV+H S+PKS+EG+YQE+GRAGRDG  + C+LFY++ DV R+++LI  +  G   T++ H   L+ MV YCEN+ +CRR Q+L +FGE+ F+   C       CDNC  + D K   RD+TD  K++V++V E         I +VG     T+N ++D+F G+ +AKI       + ++G GS +S+++AER F  L+ +  L E + +   D  +A+V+ G K Q +L+GN  V  M      + K +  ++ K  V + + + K+C  +L EV +       + +Y N+F+  TL +++E L +D   L  I    + K+ KYG   + V + Y+
Sbjct:  649 FPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTVVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATNIYLQLSKKDPIIKLLYVTPEKICASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKIL-RPQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQRDGLAALAYHAGLSDSARDEVQQKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISHCLLFYTYHDVTRLKRLIMMEKDGNHHTRETHFNNLYSMVHYCENITECRRIQLLAYFGENGFNPDFCKKHPDVSCDNCCKTKDYKT--RDVTDDVKSIVRFVQEHSSSQGMRNIKHVGPSGRFTMNMLVDIFLGSKSAKI------QSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQAIAYVMLGNKAQTVLNGNLKVDFMETENSSSVKKQKALVAK--VSQREEMVKKCLGELTEVCKSLGKVFGV-HYFNIFNTVTLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVISVLQKYS 1286          
BLAST of ATP-dependent DNA helicase vs. UniProt/SwissProt
Match: sp|Q9VGI8|BLM_DROME (Bloom syndrome protein homolog OS=Drosophila melanogaster OX=7227 GN=Blm PE=1 SV=1)

HSP 1 Score: 582.022 bits (1499), Expect = 0.000e+0
Identity = 310/729 (42.52%), Postives = 431/729 (59.12%), Query Frame = 2
Query:  971 DDVFESIDESLFIADTENFVGQLNAYRNENAPMINVTNAADLDGTSYVPINEK---QSKWAIEDFEYKSHPNDGETAEFSGD-FPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVMEISNVGWGSTLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISKPRTTKDKDKVIGK----------EPVKEVDRLQKECYQKLVEVARKFANEAKIQNYANLFSNQTLMEMSERLPADLNDLKDITSFPQVKINKYGCNFLEVTKHY 3115
            DD F   DE++F    E    Q  A ++       V++A      S VP  +     S+    +F    H NDG T EF G  F H+ R+M      FGL  FR NQ + IN+TL G D FV+MPTGGGKSLCYQLPA++TEGVTIVISPLKSLI DQ+ KL  LDI A  LSG+ K A+V  I   L+SQ   +KLLYVTPEK+  S +  +   +LN    +SRFVIDEAHCVSQWGHDFRP Y+ LG+L+  FPNVP IALTATAT +VR DIL QL +K N KWF+ SFNR NLR+ + PK     + +I   + +K +  SGI+YCLSR ECDE +  M + G++  +YHAG++DT R+  Q+ W+     +++ AT+AFGMGIDKPDVRFV+HYS+PKSIEG+YQE GRAGRDG  A C+L+Y+++D++R++K++ SD +     KK H   L+ +V YCENL DCRR Q L++FGEHF  + C   +   CDNC N    K V  D  +HA+   + V ++ +     TL ++ DV +G+   KI++        +G    + KND  R    +V +G+L E ++ T  DF  A++  G     L+ G    +  ++K    K+    +G           +    +  + + CY  L+++ R  A++  +   A++ + Q L  M+E LP    D+  I    +   +KYG   LE+T +Y
Sbjct:  647 DDGFPEYDEAMF----EQMHSQAAANKSR------VSSAGPSTSKSVVPTKQTSALHSQKLSGNFHANVH-NDGITGEFDGQKFEHSTRLMHGLSYSFGLKSFRPNQLQVINATLLGNDCFVLMPTGGGKSLCYQLPAILTEGVTIVISPLKSLIFDQINKLASLDICAKSLSGEQKMADVMAIYRDLESQPPMVKLLYVTPEKISSSARFQDTLDTLNSNNYISRFVIDEAHCVSQWGHDFRPDYKKLGVLKKRFPNVPTIALTATATPRVRLDILAQLNLK-NCKWFLSSFNRSNLRYRVLPKKGVSTLDDISRYIRSKPQHFSGIIYCLSRKECDETSKKMCKDGVRAVSYHAGLTDTDRESRQKDWLT-GKMRVICATVAFGMGIDKPDVRFVLHYSLPKSIEGYYQEAGRAGRDGDVADCILYYNYSDMLRIKKMLDSDKALQYNVKKIHVDNLYRIVGYCENLTDCRRAQQLDYFGEHFTSEQCLENRETACDNCINKRAYKAV--DALEHARKAARAVKDLCSGRSRFTLLHIADVLKGSKIKKIIDFNHHKTPHHGVLKDWDKNDVHRLLRKMVIDGFLREDLIFTN-DFPQAYLYLGNNISKLMEGTPNFEFAVTK--NAKEAKAAVGSVSDGATSSTADGQSGMREIHERCYTDLLDLCRTIASQRNV-TMASIMNIQALKSMAETLPITEKDMCSIPHVTKANFDKYGAKLLEITSNY 1356          
BLAST of ATP-dependent DNA helicase vs. UniProt/SwissProt
Match: sp|Q9DEY9|BLM_XENLA (Bloom syndrome protein homolog OS=Xenopus laevis OX=8355 GN=blm PE=2 SV=1)

HSP 1 Score: 576.63 bits (1485), Expect = 0.000e+0
Identity = 307/706 (43.48%), Postives = 438/706 (62.04%), Query Frame = 2
Query: 1196 FSG-DFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGE-HFDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVME----ISNVGWGS------TLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISKPRTTKDKDKVIGKEPVKEVDRLQKECYQKLVEVARKFANEAKIQNYANLFSNQTLMEMSERLPADLNDLKDITSFPQVKINKYGCNFLEVTKHYNLLLTKHKQEMKDLEKKLTVDIKPENA--------KSSYFSSTSKAPAKGYNRK 3253
            F G +FPH++ MM  F + FGL+ FR NQ EAIN+ L G D F++MPTGGGKSLCYQLP  I+ GVTIVISPL+SLI DQV KL  LDI AT+L+GD  +AE   I LQL  +   IKLLYVTPEKV  S +L++  ++L +R+LL+RFVIDEAHCVSQWGHDFRP Y+ L +LR  F +VPM+ALTATA  +V++DIL QLKM    + F  SFNR NL++E+ PK  K+   + +  +     + SGI+YCLSR+ECD +A  + + GL   AYHAG++D+ R  VQ  WIN++ C+++ ATIAFGMGIDKPDVR+V+H S+PKS+EG+YQE+GRAGRDG  + C+LFYS+ DV R+R+LI  +  G   TK+ H   L+ MV YCEN+++CRR Q+L++FGE +F+   C       CDNC    + K   RD+TD    +V++V +    +   G G       TLN ++D+F G+ +AKI         L+G G+ +S+++AER F  LV +  + E++ +T  D  VA+V  G + Q +L+G   V    ++  ++  K K        + + + K+C  +L E+ ++      +  Y N+F+  T+  ++E L  +   L  I    + K++KYG   ++V + Y+      +   +       V  +  N+        KSSYFSS +K   KG  RK
Sbjct:  596 FRGFNFPHSKEMMKIFHKKFGLHRFRTNQLEAINACLCGEDCFILMPTGGGKSLCYQLPGCISPGVTIVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDAEAASIYLQLSKKDPIIKLLYVTPEKVCASTRLISTMENLYERQLLARFVIDEAHCVSQWGHDFRPDYKRLNVLRQKFQSVPMMALTATANPRVKKDILNQLKM-TKPQIFTMSFNRDNLKYEVLPKKPKRVALDCVEWIKKHHPNDSGIIYCLSRHECDTMADTLQKEGLAALAYHAGLADSNRDYVQHKWINQDDCQVICATIAFGMGIDKPDVRYVIHASLPKSVEGYYQESGRAGRDGETSHCLLFYSYHDVTRIRRLIQMEKDGNSHTKQTHFNNLYSMVHYCENVVECRRMQLLSYFGENNFNPNFCKEHTQVACDNCLGKKNYK--SRDVTDDVGNIVRFVQDNCSLVQGRGKGRSNNTRLTLNMMVDIFLGSKSAKI------QTGLFGKGAAYSRHNAERLFRKLVLDRIIDEELYITFNDQAVAYVKMGERAQAVLNGFLKVDFQDTESASSIRKQKASVVTNTSQREEMVKKCQAELTELCKRLGKIFGVH-YFNIFNTATIRRIAESLSPEPEVLLQIDGVTEDKLDKYGAELIDVLQKYSEWTLPVEDICQKSGGPANVSARRSNSDHDDESCDKSSYFSSNNK---KGPKRK 1288          
BLAST of ATP-dependent DNA helicase vs. UniProt/SwissProt
Match: sp|O88700|BLM_MOUSE (Bloom syndrome protein homolog OS=Mus musculus OX=10090 GN=Blm PE=1 SV=1)

HSP 1 Score: 574.318 bits (1479), Expect = 0.000e+0
Identity = 295/648 (45.52%), Postives = 418/648 (64.51%), Query Frame = 2
Query: 1205 DFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEH-FDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVMEIS------NVGWGS--TLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQ-MPISKPRTTKDKDKVIGKEPVKEVDRLQKECYQKLVEVARKFANEAKIQNYANLFSNQTLMEMSERLPADLNDLKDITSFPQVKINKYGCNFLEVTKHYN 3118
            +FPHT+ MM  F + FGL+ FR NQ EAIN+ L G D F++MPTGGGKSLCYQLPA ++ GVTIVISPL+SLI DQV KL   DI AT+L+GD  ++E   I LQL  +   IKLLYVTPEKV  S +L++  ++L +RKLL+RFVIDEAHCVSQWGHDFR  Y+ + +LR  FP+VP++ALTATA  +V++DIL QLK+    + F  SFNR NL++ + PK  KK   + +  +       SGI+YCLSR ECD +A  + + GL   AYHAG+SD+ R +VQ  WIN++ C+++ ATIAFGMGIDKPDVRFV+H S+PKS+EG+YQE+GRAGRDG  + CVLFY++ DV R+++LI  +  G   TK+ H   L+ MV YCEN+ +CRR Q+L +FGE  F+   C       CDNC  + D K   +D+TD  K ++++V E S      N+G     TLN ++D+F G+ +AK+  K G    ++G G+ +S+++AER F  L+ +  L E + +   D  +A+V+ G K   +LSG+  V  M      + K +  ++ K  V + + + K+C  +L EV +       + +Y N+F+  TL +++E L +D   L  I    + K+ KYG   + V + Y+
Sbjct:  656 NFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTIVISPLRSLIVDQVQKLTSFDIPATYLTGDKTDSEAANIYLQLSKKDPIIKLLYVTPEKVCASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKIL-RPQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQREGLAALAYHAGLSDSARDEVQHKWINQDNCQVICATIAFGMGIDKPDVRFVIHASLPKSMEGYYQESGRAGRDGEISHCVLFYTYHDVTRLKRLIMMEKDGNYHTKETHVNNLYSMVHYCENITECRRIQLLAYFGEKGFNPDFCKKYPDVSCDNCCKTKDYKT--KDVTDDVKNIIRFVQEHSSSPGTRNIGPAGRFTLNMLVDIFLGSKSAKV--KSG----IFGKGTTYSRHNAERLFKKLILDKILDEDLYINANDQPIAYVMLGTKAHSVLSGHLKVDFMETENSSSIKKQKALVAK--VSQREEVVKKCLGELTEVCKLLGKVFGV-HYFNIFNTATLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVIPVLQKYS 1291          
BLAST of ATP-dependent DNA helicase vs. UniProt/SwissProt
Match: sp|Q9I920|BLM_CHICK (Bloom syndrome protein homolog OS=Gallus gallus OX=9031 GN=BLM PE=1 SV=2)

HSP 1 Score: 570.081 bits (1468), Expect = 0.000e+0
Identity = 290/649 (44.68%), Postives = 418/649 (64.41%), Query Frame = 2
Query: 1208 FPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGE-HFDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVMEIS----------NVGWGS-TLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISKPRTTKDKDKVIGKEPVKEVDRLQKECYQKLVEVARKFANEAKIQNYANLFSNQTLMEMSERLPADLNDLKDITSFPQVKINKYGCNFLEVTKHYN 3118
            F H+E M+  F   FGL+ FR NQ EAIN+ L G D F++MPTGGGKSLCYQLPA ++ GVT+VISPL+SLI DQV KL+ LDI +T+L+GD+ +A+  K  +QL  +   IKLLYVTPEKV  S +L++  ++L  RKLL+RFVIDEAHCVSQWGHDFR  Y+ L +LR  F +VPM+ALTATA  +V++DI  QL+M    + F  SFNR NL++++ PK  KK   + +  +       SGI+YCLSR+ECD  A+++ + GL   AYHAG++D+ R  VQ+ W+N+ GC+++ ATIAFGMGIDKPDVR+V+H S+PKSIEG+YQE+GRAGRDG  + C+LFYS++DV R+R+LI  +  G   T++ H   L+ MV YCEN++DCRR Q+L +FGE  F+   C      ICDNC    D K   R++TD  K+++++V +            N G G  TLN ++D+F GA +AKI       + ++G G+ +S+++ ER F  LV +  L E + +T  D  VA+VV G K Q +L+G   V+   ++  +   K +    + + + + + K+C  +L +  +       + +Y N+FS  TL +++E L +D   L  I    + K+ KYG   ++V   Y+
Sbjct:  420 FSHSEEMLKIFHRKFGLHSFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSAGVTVVISPLRSLIIDQVQKLKTLDIASTYLTGDITDADASKTYMQLSKKDPIIKLLYVTPEKVCASNRLLSALENLYNRKLLARFVIDEAHCVSQWGHDFRKDYKRLNMLRKKFHSVPMMALTATANPRVQKDIQNQLEML-KPQVFTMSFNRHNLKYDVLPKKPKKVAMDCLEWIKKYHPHDSGIIYCLSRHECDTTAAILQKEGLAALAYHAGLTDSNRDLVQKKWVNQEGCQVICATIAFGMGIDKPDVRYVIHASLPKSIEGYYQESGRAGRDGEMSHCLLFYSYSDVTRLRRLILMEKDGNSHTRQTHFNNLYSMVHYCENVVDCRRIQLLAYFGETDFNPNFCKDHPEVICDNCSRKKDYK--SRNVTDEVKSIIRFVQQHCGQVGGINGNRNTGSGRYTLNMMVDIFLGAKSAKI------QSGIFGKGAAYSRHNVERLFRKLVLDKILDEDLYITANDQAVAYVVLGEKAQAVLNGLLQVEFHETENASAIRKQRASVTK-MSQREEMVKKCLGELTDTCKTLGKIFDV-HYFNIFSTSTLKKIAETLSSDAEVLLQIDGVTEDKLEKYGAEIIKVMDKYS 1057          
BLAST of ATP-dependent DNA helicase vs. TrEMBL
Match: A0A074Z7F7 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_08929 PE=4 SV=1)

HSP 1 Score: 793.497 bits (2048), Expect = 0.000e+0
Identity = 413/874 (47.25%), Postives = 555/874 (63.50%), Query Frame = 2
Query:  635 VRITQKYHDLLDNFFGYLEQFPISKLVEFFDDKSSLVSEILTERNINK--KAMLALSDRSIVLESSSF---QSITPGNSYGDIFDKDETVVDFDEQDEICENQFEPSVKSFDEWED--GD-DVFESIDESLFIADTENFVGQLNAYRNENAP-------MINVT----NAADLDGTSYVPINEKQSKW--AIEDFEYKSHPNDGETAEFSG--DFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSG--TIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVMEISNVGWGSTLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISKP-RTTKDKDKVIGKEPVKEVDRLQKECYQKLVEVARKFANEAKIQNYANLFSNQTLMEMSERLPADLNDLKDITSFPQVKINKYGCN--FLEVTKHYNLLLTKHKQEMKDLEKKLT 3172
            V IT++ ++L+ N    +E  P++KL+  F D  + +S+ L ERN NK  +   A ++ + V   +S      + P         ++           I + +  P+  S    ED  GD D  ESI     I  T   V  +     E  P       ++  T    +A   +G  ++P++   SKW   +       HP+DG T EF G   FPH+E++M AF ++FGL  FRRNQ +AIN+ L G D FVIMPTGGGKSLCYQLPA + +G+T+VISPLK+L+ DQVTK+Q L IVA HL+G+    E D++   L    ++ KLLYVTPEK+  S KL    + L +R LL RFVIDEAHCVSQWGHDFRP YR L +LRT FP VPM+A+TATAT +VREDIL+QLKMK NTKWFIQSFNR NL++E+RPK  K C  EII +++++F  +SGIVYCLSR ECD VA  ++++GL   AYHAGM+D +R+++QE WI E+ CKIV ATIAFGMGIDKPDVRFV+H+S+PKSIEG+YQE GR+GRDGLPATC+L+Y W DVVR+RKLI  D  G  T    + HE+ LF MVSYCEN IDCRR Q+L+HFGE FD   CG V GC+CDNCQ ++  ++  RD+T+ A  +V+ V          T+NY +DVFRGA  ++I   Q   N LY  G+ +SK DAER FH LV E  L+E++ VT  D VVA+V PG K   LL G+  + +PIS P R    + K+IG+EP     +++ ECY++LV+VA++  +E  I NYA +F N+ L+EM+  LP    +L  +    + K+N++     FLE+T +Y  +L   K E +  E +LT
Sbjct:  181 VVITRRLYELMQNVCDAIESLPMNKLLACFGDNLTTISQFLHERN-NKGMEPTSATANYTFVDPITSPIRPSRMEPKKPSTPSTLREAMASACKPTTPINQARLPPTSSSISRTEDSWGDFDFGESI-----IKTTRKPVQSVPGESVEKLPKPPQTTHLVEATRPVLHADTGEGGVFLPLSTTFSKWEAPVSASHRDDHPDDGSTGEFDGTDKFPHSEKVMEAFSKLFGLKSFRRNQLQAINAALLGLDCFVIMPTGGGKSLCYQLPAAVQDGLTLVISPLKALVLDQVTKMQSLGIVAAHLTGEATMTESDRVYASLHLATLRTKLLYVTPEKIAASDKLKGCLEQLYRRNLLQRFVIDEAHCVSQWGHDFRPDYRNLSILRTNFPKVPMMAMTATATPRVREDILHQLKMK-NTKWFIQSFNRTNLKFEVRPKKVKSCTKEIIEVIHSEFPRRSGIVYCLSRRECDLVAEELSRAGLAASAYHAGMTDAQRRRIQEAWIQEDKCKIVCATIAFGMGIDKPDVRFVIHHSLPKSIEGYYQEAGRSGRDGLPATCILYYHWHDVVRLRKLIQGDTPGSNTFANIQLHEEALFRMVSYCENQIDCRRRQILSHFGEAFDAADCGLVVGCMCDNCQQADRRRLEQRDVTEDAVLIVRAVDGFVRSRRNVTINYCVDVFRGAQTSQIQRAQDNLNPLYNRGASYSKTDAERLFHRLVAESVLYEELTVTHQDHVVAYVRPGSKAVRLLEGSLKIHLPISMPSRPGAAEAKMIGREPEDRYAKIRNECYEELVQVAKQLTSEQGISNYATVFPNEMLLEMAAHLPTTREELLQVPQCTEYKLNRFNAQDRFLEITLNYLSILGALKSEEQQQEAQLT 1047          
BLAST of ATP-dependent DNA helicase vs. TrEMBL
Match: A0A4S2M6C7 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_002409 PE=4 SV=1)

HSP 1 Score: 791.571 bits (2043), Expect = 0.000e+0
Identity = 409/878 (46.58%), Postives = 549/878 (62.53%), Query Frame = 2
Query:  635 VRITQKYHDLLDNFFGYLEQFPISKLVEFFDDKSSLVSEILTERNINKKAMLALSDRSIVLESSSFQSITPGNSYGDIFDKDETVVDFDE---------QDEICENQFEPSVKSFDEWED--GD-DVFESIDESLFIADTENFVGQLNAYRNENAP-----------MINVTNAADLDGTSYVPINEKQSKW--AIEDFEYKSHPNDGETAEFSG--DFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGT--IETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVMEISNVGWGSTLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISKP-RTTKDKDKVIGKEPVKEVDRLQKECYQKLVEVARKFANEAKIQNYANLFSNQTLMEMSERLPADLNDLKDITSFPQVKINKYGCN--FLEVTKHYNLLLTKHKQEMKDLEKKLT 3172
            V IT++ ++L+ +    +E  P++KL+  F D  + +S+ L ERN N+    A+  +S     +S   IT       +  K  +                  I + +  P+  S    ED  GD D  ESI +S     T   +  L +   +  P              V +A   +G  ++P++   SKW   +       HP+DG T EF G   FPH+ +MM AF ++FGL  FRRNQ +AIN+ L G D FVIMPTGGGKSLCYQLPA + +G+T+VISPLK+L+ DQVTK+Q L IVA HL+G+    E D++   L    ++ KLLYVTPEK+  S KL    + L +R LL RFVIDEAHCVSQWGHDFRP YR L +LRT FP VPM+A+TATAT +VREDIL+QLKMK NTKWFIQSFNR NL++E+RPK  K C  EII +++++F  +SGIVYCLSR ECD VA  + ++GL   AYHAGM+D +R+++QE WI E+ CKIV ATIAFGMGIDKPDVRFV+H+S+PKSIEG+YQE GR+GRDGLPATC+L+Y W DVVR+RKLI  D  G+      + HE+ LF MVSYCEN IDCRR Q+L+HFGE FD   CG V GC+CDNCQ ++  ++  RD+T+ A  +V+ V          T+NY +DVFRGA  ++I   Q   N LY  G+ +SK DAER  H LV E  L+E++ VT  D VVA+V PG K   LL G+  + +PIS P R    + K+IG+EP     +++ ECY++LV+VA++  +E  I NYA +F N  L+EM+  LP    +L  +    + K+N++     FLE+T +Y  +L   K E +  E + T
Sbjct:  205 VVITRRLYELMQSVCDAIESLPMNKLLACFGDNLTTISQFLHERN-NR----AMEPKSATANYASVDPITSPTRPSRMEPKKPSTPSTPHGTMASVCKPTTPISQARLPPTSSSISRTEDSWGDFDFGESITKS-----TRTPIQSLPSESVKKLPKPPQTTQLFEAARPVLHADTGEGGVFLPLSTTLSKWEAPVSASHRDDHPDDGSTGEFDGTDKFPHSAKMMEAFSKLFGLKSFRRNQLQAINAALLGLDCFVIMPTGGGKSLCYQLPAAVQDGLTLVISPLKALVLDQVTKMQSLGIVAAHLTGEATMTESDRVYASLHLATLRTKLLYVTPEKIAASDKLKGCLEQLYRRNLLQRFVIDEAHCVSQWGHDFRPDYRNLSILRTNFPKVPMMAMTATATPRVREDILHQLKMK-NTKWFIQSFNRTNLKFEVRPKKLKSCTKEIIEVIHSEFPRRSGIVYCLSRRECDLVAEELCRAGLAASAYHAGMTDAQRRRIQEAWIQEDKCKIVCATIAFGMGIDKPDVRFVIHHSLPKSIEGYYQEAGRSGRDGLPATCILYYHWHDVVRLRKLIQGDTPGSNAFANIQLHEEALFRMVSYCENQIDCRRRQILSHFGEAFDAADCGLVVGCMCDNCQQADRRRLEQRDVTEDAVLIVRTVDGFVRSRRNVTINYCVDVFRGAQTSQIQRAQDNLNPLYSRGASYSKTDAERLLHRLVAESVLYEELTVTHQDHVVAYVRPGSKAVRLLEGSLKIHLPISMPSRPGAAEAKMIGREPEDRYAKIRNECYEELVQVAKQMTSEQGISNYATVFPNDMLLEMAAHLPTTREELLQVPQCTEYKLNRFNAQDRFLEITLNYLSILGALKSEEQQQETQST 1071          
BLAST of ATP-dependent DNA helicase vs. TrEMBL
Match: A0A3R7F8K6 (Bloom syndrome-like protein (Fragment) OS=Clonorchis sinensis OX=79923 GN=blm PE=4 SV=1)

HSP 1 Score: 786.563 bits (2030), Expect = 0.000e+0
Identity = 402/861 (46.69%), Postives = 545/861 (63.30%), Query Frame = 2
Query:  635 VRITQKYHDLLDNFFGYLEQFPISKLVEFFDDKSSLVSEILTERN----------INKKAMLALSDRSIVLESSSFQSITPGNSYGDIFDKDETVVDFDEQDEICENQFEPSVKSFDEWED--GD-DVFESIDESLFIADTENFVGQLNAYRNENAPMIN--------VTNAADLDGTSYVPINEKQSKW--AIEDFEYKSHPNDGETAEFSG--DFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGT--IETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVMEISNVGWGSTLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISKP-RTTKDKDKVIGKEPVKEVDRLQKECYQKLVEVARKFANEAKIQNYANLFSNQTLMEMSERLPADLNDLKDITSFPQVKINKYGCN--FLEVTKHYNLLL 3127
            V IT++ ++L+ N    +E  P++KL+  F D  + VS+ L ER            N  +   ++  +        +  TP   +G +    +     ++       +  P+  S    ED  GD D  ESI +S      ++  G+ +  +    P           V +A   +G  ++P++   SKW   +       HP+DG T EF G   FPH+ +MM AF ++FGL  FRRNQ +AIN+ L G D FVIMPTGGGKSLCYQLPA + +G+T+VISPLK+L+ DQVTK+Q L IVA HL+G+    E D++   L    ++ KLLYVTPEK+  S KL    + L +R LL RFVIDEAHCVSQWGHDFRP YR L +LRT FP VPM+A+TATAT +VREDIL+QLKMK NTKWFIQSFNR NL++E+RPK  K C  EII +++++F  +SGIVYCLSR ECD VA  ++++GL   AYHAGM+D +R+++QE WI E+ CKIV ATIAFGMGIDKPDVRFV+H+S+PKSIEG+YQE GR+GRDGLPATC+L+Y W DVVR+RKLI  D  G+      + HE+ LF MVSYCEN IDCRR Q+L+HFGE FD   CG V GC+CDNCQ ++  ++  RD+T+ A  +V+ V          T+NY +DVFRGA  ++I   Q   N LY  G+ +SK DAER FH LV E  L+E++ VT  D VVA+V PG K   LL G+  + +PIS P R    + K+IG+EP     +++ ECY++LV+VA++  +E  I NYA +F N+ L+EM+  LP    +L  +    + K+N++     FLE+T +Y  +L
Sbjct:  198 VVITRRLYELMQNVCDVIESLPMNKLLACFGDNLTTVSQFLHERTNKGMEPTSATANHASADPITSPTRPSRMEPKKPSTPSTHHGAMASGCKPATPINQV------RLPPTSSSISRTEDSWGDFDFGESITKSTR-KPVQSVPGE-SVKKLPKPPQTTHLVEATRPVLHADTGEGGVFLPLSTTLSKWEAPVAASHRDDHPDDGSTGEFDGTDKFPHSAKMMEAFSKLFGLKSFRRNQLQAINAALLGLDCFVIMPTGGGKSLCYQLPAAVQDGLTLVISPLKALVLDQVTKMQSLGIVAAHLTGEATMTESDRVYASLHLATLRTKLLYVTPEKIAASDKLKGCLEQLYRRNLLQRFVIDEAHCVSQWGHDFRPDYRNLSILRTNFPKVPMMAMTATATPRVREDILHQLKMK-NTKWFIQSFNRTNLKFEVRPKKLKSCTKEIIEVIHSEFPRRSGIVYCLSRRECDLVAEELSRAGLAASAYHAGMTDAQRRRIQEAWIQEDKCKIVCATIAFGMGIDKPDVRFVIHHSLPKSIEGYYQEAGRSGRDGLPATCILYYHWHDVVRLRKLIQGDTPGSNAFANVQLHEEALFRMVSYCENQIDCRRRQILSHFGEAFDAADCGLVVGCMCDNCQQADRRRLEQRDVTEDAVLIVRAVDGFVRSRRNVTINYCVDVFRGAQTSQIQRAQDNLNPLYNRGASYSKTDAERLFHRLVAESVLYEELTVTHQDHVVAYVRPGSKAVRLLEGSLKIHLPISMPSRPGAAEAKMIGREPEDRYAKIRNECYEELVQVAKQMTSEQGISNYATVFPNEMLLEMAAHLPTTREELLQVPQCTEYKLNRFNAQDRFLEITLNYLSIL 1049          
BLAST of ATP-dependent DNA helicase vs. TrEMBL
Match: H2KNK1 (ATP-dependent DNA helicase OS=Clonorchis sinensis OX=79923 GN=CLF_100333 PE=3 SV=1)

HSP 1 Score: 779.63 bits (2012), Expect = 0.000e+0
Identity = 402/869 (46.26%), Postives = 543/869 (62.49%), Query Frame = 2
Query:  665 LDNFFGYLEQFPISKLVEFFDDKSSLVSEILTERN----------INKKAMLALSDRSIVLESSSFQSITPGNSYGDIFDKDETVVDFDEQDEICENQFEPSVKSFDEWED--GD-DVFESIDESLFIADTENFVGQLNAYRNENAP-------MINVT----NAADLDGTSYVPINEKQSKW--AIEDFEYKSHPNDGETAEFSG--DFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGT--IETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVMEISNVGWGSTLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISKP-RTTKDKDKVIGKEPVKEVDRLQKECYQKLVEVARKFANEAKIQNYANLFSNQTLMEMSERLPADLNDLKDITSFPQVKINKYGCN--FLEVTKHYNLLLTKHKQEMKDLEKKLT 3172
            + N    +E  P++KL+  F D  + VS+ L ER            N  +   ++  +        +  TP   +G +    +     ++       +  P+  S    ED  GD D  ESI +S     T   V  +     +  P       ++  T    +A   +G  ++P++   SKW   +       HP+DG T EF G   FPH+ +MM AF ++FGL  FRRNQ +AIN+ L G D FVIMPTGGGKSLCYQLPA + +G+T+VISPLK+L+ DQVTK+Q L IVA HL+G+    E D++   L    ++ KLLYVTPEK+  S KL    + L +R LL RFVIDEAHCVSQWGHDFRP YR L +LRT FP VPM+A+TATAT +VREDIL+QLKMK NTKWFIQSFNR NL++E+RPK  K C  EII +++++F  +SGIVYCLSR ECD VA  ++++GL   AYHAGM+D +R+++QE WI E+ CKIV ATIAFGMGIDKPDVRFV+H+S+PKSIEG+YQE GR+GRDGLPATC+L+Y W DVVR+RKLI  D  G+      + HE+ LF MVSYCEN IDCRR Q+L+HFGE FD   CG V GC+CDNCQ ++  ++  RD+T+ A  +V+ V          T+NY +DVFRGA  ++I   Q   N LY  G+ +SK DAER FH LV E  L+E++ VT  D VVA+V PG K   LL G+  + +P+S P R    + K+IG+EP     +++ ECY++LV+VA++  +E  I NYA +F N+ L+EM+  LP    +L  +    + K+N++     FLE+T +Y  +L   K E +  E + T
Sbjct:    1 MQNVCDVIESLPMNKLLACFGDNLTTVSQFLHERTNKGMEPTSATANHASADPITSPTRPSRMEPKKPSTPSTHHGAMASGCKPATPINQV------RLPPTSSSISRTEDSWGDFDFGESITKS-----TRKPVQSVPGESVKKLPKPPQTTHLVEATRPVLHADTGEGGVFLPLSTTLSKWEAPVAASHRDDHPDDGSTGEFDGTDKFPHSAKMMEAFSKLFGLKSFRRNQLQAINAALLGLDCFVIMPTGGGKSLCYQLPAAVQDGLTLVISPLKALVLDQVTKMQSLGIVAAHLTGEATMTESDRVYASLHLATLRTKLLYVTPEKIAASDKLKGCLEQLYRRNLLQRFVIDEAHCVSQWGHDFRPDYRNLSILRTNFPKVPMMAMTATATPRVREDILHQLKMK-NTKWFIQSFNRTNLKFEVRPKKLKSCTKEIIEVIHSEFPRRSGIVYCLSRRECDLVAEELSRAGLAASAYHAGMTDAQRRRIQEAWIQEDKCKIVCATIAFGMGIDKPDVRFVIHHSLPKSIEGYYQEAGRSGRDGLPATCILYYHWHDVVRLRKLIQGDTPGSNAFANVQLHEEALFRMVSYCENQIDCRRRQILSHFGEAFDAADCGLVVGCMCDNCQQADRRRLEQRDVTEDAVLIVRAVDGFVRSRRNVTINYCVDVFRGAQTSQIQRAQDNLNPLYNRGASYSKTDAERLFHRLVAESVLYEELTVTHQDHVVAYVRPGSKAVRLLEGSLKIHLPMSMPSRPGAAEAKMIGREPEDRYAKIRNECYEELVQVAKQMTSEQGISNYATVFPNEMLLEMAAHLPTTREELLQVPQCTEYKLNRFNAQDRFLEITLNYLSILGALKSEEQQQEAQST 857          
BLAST of ATP-dependent DNA helicase vs. TrEMBL
Match: A0A4Z2D6W0 (Bloom syndrome protein OS=Schistosoma japonicum OX=6182 GN=EWB00_003961 PE=4 SV=1)

HSP 1 Score: 772.696 bits (1994), Expect = 0.000e+0
Identity = 405/894 (45.30%), Postives = 568/894 (63.53%), Query Frame = 2
Query:  629 TGVRITQKYHDLLDNFFGYLEQFPISKLVEFFDDKSSLVSEILTERNINKKAMLALSDRSIVLESSSFQSITPGNSYGDIFDKDETVVDFDEQDEI--CENQFEPSVKSFDEWEDGDDVFESIDESLFIADTENFVGQLNAYRNENAPMINVTNAADLD---GTSYVPINEKQSKWAIEDF--EYKSHPNDGETAEFSGD--FPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSD--NSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVMEISNVGWGSTLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISKP-RTTKDKDKVIGKEPVKEVDRLQKECYQKLVEVARKFANEAKIQNYANLFSNQTLMEMSERLPADLNDLKDITSFPQVKINKYGCN--FLEVTKHY-NLL--LTKHKQEMKD-LEKKLTVDIKPENAKSSYFSSTSKAPAKGYNRKR 3256
            + V IT+ ++  +      LE  P++KL+  F D+ S+++++L ER++N        + S   +S + + I+   S   I ++    ++  ++ EI       E  +   D W + +         L ++     + +      +    I  ++   +D   G+ ++P+ + QSKW +     +  + P+DG T EF GD  FPH++RMM AF ++FGL  FRRNQ +AIN+ L G D FVIMPTGGGKSLCYQLPAV+  G+T+VISPLK+L+ DQVTKLQ L + A HL+G+   +E DK+   L +  +++KLLYVTPEK+  S KL    + L +RKLL RFVIDEAHCVSQWGHDFRP YR L +LRT FP+VPM+A+TATAT KVR DIL QLKM  NTKWFIQSFNR NLR+E+RPK  K C  EII+++  +F  +SGIVYCLSR ECD VA  +  SGL+  AYHAGM+D +R+ VQ+ WI E+ CKIV ATIAFGMGIDKPDVRFV+H+S+PKSIEG+YQE GR+GRDGLP+TC+L+Y W DVVR+RKLI  D  +SG  ET + HE  L+ MVSYCEN IDCRR Q+L HFGE FD   CG + GC+CDNCQ S+  ++  RD+TD A  +V+ V          T+NY++D+FRGA  ++I +     N +YG GS +SK DAER FH LV E  L+E++ VTQ D VVA+V  G +   LL+G+  + +P+S P R+   +  ++G+ P      ++ +CY++LV+ A++  +   I NYA +F N+ L+EM+  LPA   +L  I      K+N +     FLE+T +Y ++L  L   +QE K+ +  + ++ +   N     F+   +   K YN K+
Sbjct:  170 SSVTITRSFYSSICKTCDLLESLPMNKLMHCFGDELSVITKLLHERSLNS------VNASSQPKSGTLKKISNVFSPVSISEQRARNINVSKKMEISVTSRSLENVLSDDDSWGNININSPRTQPRLDVSTKCPVIKKSTVTPKKAVNGITQSSTLTVDSGEGSVFLPL-KVQSKWDVPTHFDDQCNQPDDGSTGEFDGDDRFPHSKRMMEAFSKMFGLRSFRRNQLQAINAALLGRDCFVIMPTGGGKSLCYQLPAVVQSGLTLVISPLKALVLDQVTKLQSLGVPAAHLTGEANLSESDKVYTALYTVNLRLKLLYVTPEKIAASDKLRGCLEQLYKRKLLDRFVIDEAHCVSQWGHDFRPDYRNLNILRTNFPDVPMMAMTATATPKVRRDILLQLKM-TNTKWFIQSFNRANLRFEVRPKKLKNCTKEIIDVIRNEFPKRSGIVYCLSRRECDVVAEELRTSGLQASAYHAGMTDAQRRNVQQAWIQEDKCKIVCATIAFGMGIDKPDVRFVIHHSLPKSIEGYYQEAGRSGRDGLPSTCILYYHWHDVVRLRKLIQGDGSSSGARETHQFHEDALYRMVSYCENQIDCRRKQMLAHFGEAFDPATCGTIVGCLCDNCQLSDRRRLEHRDVTDDAIKIVRTVETFVRSRRNVTINYLVDIFRGAKTSQIQKSGDEANQIYGKGSAYSKTDAERLFHRLVSERILYEELAVTQLDHVVAYVRLGLRSTGLLNGSIKITLPVSIPNRSGPSETNIVGRPPEDRYANIRNQCYEELVQAAKQLTSSQGISNYATVFPNEMLLEMASSLPATREELLQIPQCTDYKLNCFNAESPFLEITLNYLSILGALNAEEQEQKEIIASQQSLPLVSSNRGRGNFAKRGR--TKAYNTKK 1053          
BLAST of ATP-dependent DNA helicase vs. Ensembl Cavefish
Match: blm (BLM RecQ like helicase [Source:NCBI gene;Acc:103043866])

HSP 1 Score: 579.326 bits (1492), Expect = 0.000e+0
Identity = 300/675 (44.44%), Postives = 429/675 (63.56%), Query Frame = 2
Query: 1127 KQSKWAIEDFEYKSHPNDGETAEFSG-DFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEH-FDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVMEISNVGWGS----------TLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISKPRTTKDKDKVIGKEPVKEVDRLQKECYQKLVEVARKFANEAKIQNYANLFSNQTLMEMSERLPADLNDLKDITSFPQVKINKYGCNFLEVTKHY 3115
            K +K A  +  Y++  +D     F G  FPH+  MM  F + FGL+ FR NQ EAIN+TL G DTFV+MPTGGGKSLCYQLPA ++ GVT+VISPL+SLI DQV KL  LDI AT LSGD  E+E  +I +QL  +   IKLLY TPEKV  S ++++  Q+L +R LL+RFVIDEAHCVSQWGHDFRP Y+ L  LR  FPNVP++ALTATAT +V++DIL Q+ M N  + F  SFNR NL++ + PK  KK   E IN +   +   SGI+YCLSRN+CD +A  + ++G+   AYHAG++D  R+ VQ  WIN++ C+++ ATIAFGMGIDKPDVR+V+H S+PKS+EG+YQE+GRAGRDG  + CVLFYS++DV+R+++++  D  G   ++  H   L  MV +CEN+ +CRR Q+L +FGE+ F+   C      ICDNC   +  K   R++T+  K +V++V E      G           TLN ++D+F GA +A+I         ++G G+ +S+++A+R F  LV +  L E + +T     VA++  G K   +L+G+  V    ++  ++  K K    + V + + +  +C ++L E+ +K      + +Y N+FS  TL  ++E L AD   L  I    + K++KYG   +E+ + Y
Sbjct:  618 KPTKPASPEVTYRNPAHD----RFRGFSFPHSSEMMKIFHKRFGLHQFRFNQLEAINATLLGEDTFVLMPTGGGKSLCYQLPACVSAGVTVVISPLRSLIVDQVQKLTTLDIPATSLSGDKSESEAGRIYMQLSKKDPVIKLLYATPEKVSASGRMISALQNLYERNLLARFVIDEAHCVSQWGHDFRPDYKRLHELRQKFPNVPIMALTATATPRVQKDILNQMAM-NRPQVFTMSFNRHNLKYSVLPKKPKKVDEECINWIKKYYPRDSGIIYCLSRNDCDTMADSLQRAGIAALAYHAGLNDKDREYVQNKWINQDRCQVMCATIAFGMGIDKPDVRYVIHASLPKSVEGYYQESGRAGRDGEISHCVLFYSYSDVIRIKRILAMDKDGNHHSRATHTNNLHSMVHFCENVSECRRIQLLAYFGENKFNADFCKEHPEVICDNCARPHQYKA--RNVTEDVKKIVRFVQENCQKVGGRYSKSSQQNRLTLNMLVDIFIGAKSARI------QTGMFGHGAAYSRHNADRLFKKLVLDNVLMEDLYITNNGQAVAYISAGQKAMNVLNGSMQVVFHETESASSIRKHKASVIKNVSKREEMVTKCLEELNELCKKLGKVFGL-HYYNIFSTSTLKTIAETLSADPEVLLQIDGVTEDKLDKYGAELIELLQKY 1278          
BLAST of ATP-dependent DNA helicase vs. Ensembl Cavefish
Match: recql (RecQ like helicase [Source:NCBI gene;Acc:103027659])

HSP 1 Score: 392.889 bits (1008), Expect = 2.255e-122
Identity = 227/634 (35.80%), Postives = 353/634 (55.68%), Query Frame = 2
Query:  947 SFDEWEDGDDVFESIDESL-----FIADTENFVGQLNAYRNENAPMINVTNAADLDGTSYVPINEKQSKWAIEDFEYKSHPNDGETAEFSGDFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVMEISNVGWGSTLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISK 2833
            S ++ E+  +  +SID  L      I++       LN+ +N+   +++ +  A     S  P     SK  ++ +E               DF  ++ +    + +F ++ FR  Q+ AIN ++SG D F++MPTG GKSLCYQLPAV ++G T+VI+PL SL+ DQ+  L+ + + A  L+    + +   ++  +  +    KLLYVTPEK+ +S+ LM+  +      LL R  +DE HC SQWGHDFRP Y+LLG+L+  FP VP++ LTATAT+ + +D    L + +        FNR NL +E+R K+++    EI  L+  +++DQSGIVY  S+ + + VA  + Q G+  Q YHA M  + + +V   W  +   ++VVAT+AFGMGIDK DVRFV+H++I KSIE +YQE+GRAGRD  PA C++FY ++D+ R+  ++  +N+G        +Q+L  MV+YC+N+  CRR  +  HF E +D + C      +CD C++ ND   V  D+T HA+ VVK V   S+V    T   V D + G  NAK+ +       +  T ++ S+ + E    HL+  GY  E    T       ++  G K  LL S N ++ M + +
Sbjct:    3 SINDKEEAREELDSIDAELELLELQISELLEKQASLNSRKNKLLKILDGSCDASQASGSGKPSKPAFSKKDLQHYE-------------ESDFSWSKDVQEKLENIFKISKFRPLQRAAINLSMSGRDLFLVMPTGRGKSLCYQLPAVCSKGFTLVIAPLVSLMEDQLMYLKSVGVSAVTLNASSSKEDSKFVMAGMTDKNSPFKLLYVTPEKIAKSKLLMSKLEKAYNMGLLERIAVDEVHCCSQWGHDFRPDYKLLGILKRQFPKVPLVGLTATATSNILKDCQKILCVPDPVT-LTAPFNRPNLYYEVRVKSSEDSNGEIAALIKGRYKDQSGIVYVFSQKDAELVAIDLQQRGILAQPYHANMEPSDKSRVHRHWSTKK-IQVVVATVAFGMGIDKADVRFVIHHTISKSIENYYQESGRAGRDDHPADCIVFYGFSDIFRISTMVVMENTG--------QQKLQNMVAYCQNVDRCRRAMMAVHFDEVWDDEECHQ----MCDVCRHGND--YVTMDITKHARDVVKLVELASSVDEKMTPLKVCDTWMGKGNAKLRK-------MIQT-TILSRQEVESIIVHLLLNGYFSEDFSFTPYT-TYFYLKAGRKAALLKSENHSITMKMKR 598          
BLAST of ATP-dependent DNA helicase vs. Ensembl Cavefish
Match: recql (RecQ like helicase [Source:NCBI gene;Acc:103027659])

HSP 1 Score: 393.275 bits (1009), Expect = 5.908e-121
Identity = 226/634 (35.65%), Postives = 351/634 (55.36%), Query Frame = 2
Query:  947 SFDEWEDGDDVFESIDESL-----FIADTENFVGQLNAYRNENAPMINVTNAADLDGTSYVPINEKQSKWAIEDFEYKSHPNDGETAEFSGDFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVMEISNVGWGSTLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISK 2833
            S ++ E+  +  +SID  L      I++       LN+ +N+   +++ +  A     S  P     SK  ++ +E               DF  ++ +    + +F ++ FR  Q+ AIN ++SG D F++MPTG GKSLCYQLPAV ++G T+VI+PL SL+ DQ+  L+ + + A  L+    + +   ++  +  +    KLLYVTPEK+ +S+ LM+  +      LL R  +DE HC SQWGHDFRP Y+LLG+L+  FP VP++ LTATAT+ + +D    L + +        FNR NL +E+R K+++    EI  L+  +++DQSGIVY  S+ + + VA  + Q G+  Q YHA M  + + +V   W +    ++VVAT+AFGMGIDK DVRFV+H++I KSIE +YQE+GRAGRD  PA C++FY ++D+ R+  ++  +N+G        +Q+L  MV+YC+N+  CRR  +  HF E +D + C      +CD C++ ND   V  D+T HA+ VVK V   S+V    T   V D + G  NAK+ +            ++ S+ + E    HL+  GY  E    T       ++  G K  LL S N ++ M + +
Sbjct:    3 SINDKEEAREELDSIDAELELLELQISELLEKQASLNSRKNKLLKILDGSCDASQASGSGKPSKPAFSKKDLQHYE-------------ESDFSWSKDVQEKLENIFKISKFRPLQRAAINLSMSGRDLFLVMPTGRGKSLCYQLPAVCSKGFTLVIAPLVSLMEDQLMYLKSVGVSAVTLNASSSKEDSKFVMAGMTDKNSPFKLLYVTPEKIAKSKLLMSKLEKAYNMGLLERIAVDEVHCCSQWGHDFRPDYKLLGILKRQFPKVPLVGLTATATSNILKDCQKILCVPDPVT-LTAPFNRPNLYYEVRVKSSEDSNGEIAALIKGRYKDQSGIVYVFSQKDAELVAIDLQQRGILAQPYHANMEPSDKSRVHRHW-STKKIQVVVATVAFGMGIDKADVRFVIHHTISKSIENYYQESGRAGRDDHPADCIVFYGFSDIFRISTMVVMENTG--------QQKLQNMVAYCQNVDRCRRAMMAVHFDEVWDDEECHQ----MCDVCRHGND--YVTMDITKHARDVVKLVELASSVDEKMTPLKVCDTWMGKGNAKLRK--------MIQTTILSRQEVESIIVHLLLNGYFSEDFSFTPYT-TYFYLKAGRKAALLKSENHSITMKMKR 598          
BLAST of ATP-dependent DNA helicase vs. Ensembl Cavefish
Match: recql (RecQ like helicase [Source:NCBI gene;Acc:103027659])

HSP 1 Score: 393.66 bits (1010), Expect = 8.186e-121
Identity = 226/636 (35.53%), Postives = 352/636 (55.35%), Query Frame = 2
Query:  941 VKSFDEWEDGDDVFESIDESL-----FIADTENFVGQLNAYRNENAPMINVTNAADLDGTSYVPINEKQSKWAIEDFEYKSHPNDGETAEFSGDFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVMEISNVGWGSTLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISK 2833
            + S ++ E+  +  +SID  L      I++       LN+ +N+   +++ +  A     S  P     SK  ++ +E               DF  ++ +    + +F ++ FR  Q+ AIN ++SG D F++MPTG GKSLCYQLPAV ++G T+VI+PL SL+ DQ+  L+ + + A  L+    + +   ++  +  +    KLLYVTPEK+ +S+ LM+  +      LL R  +DE HC SQWGHDFRP Y+LLG+L+  FP VP++ LTATAT+ + +D    L + +        FNR NL +E+R K+++    EI  L+  +++DQSGIVY  S+ + + VA  + Q G+  Q YHA M  + + +V   W +    ++VVAT+AFGMGIDK DVRFV+H++I KSIE +YQE+GRAGRD  PA C++FY ++D+ R+  ++  +N+G        +Q+L  MV+YC+N+  CRR  +  HF E +D + C      +CD C++ ND   V  D+T HA+ VVK V   S+V    T   V D + G  NAK+ +            ++ S+ + E    HL+  GY  E    T       ++  G K  LL S N ++ M + +
Sbjct:    1 MASINDKEEAREELDSIDAELELLELQISELLEKQASLNSRKNKLLKILDGSCDASQASGSGKPSKPAFSKKDLQHYE-------------ESDFSWSKDVQEKLENIFKISKFRPLQRAAINLSMSGRDLFLVMPTGRGKSLCYQLPAVCSKGFTLVIAPLVSLMEDQLMYLKSVGVSAVTLNASSSKEDSKFVMAGMTDKNSPFKLLYVTPEKIAKSKLLMSKLEKAYNMGLLERIAVDEVHCCSQWGHDFRPDYKLLGILKRQFPKVPLVGLTATATSNILKDCQKILCVPDPVT-LTAPFNRPNLYYEVRVKSSEDSNGEIAALIKGRYKDQSGIVYVFSQKDAELVAIDLQQRGILAQPYHANMEPSDKSRVHRHW-STKKIQVVVATVAFGMGIDKADVRFVIHHTISKSIENYYQESGRAGRDDHPADCIVFYGFSDIFRISTMVVMENTG--------QQKLQNMVAYCQNVDRCRRAMMAVHFDEVWDDEECHQ----MCDVCRHGND--YVTMDITKHARDVVKLVELASSVDEKMTPLKVCDTWMGKGNAKLRK--------MIQTTILSRQEVESIIVHLLLNGYFSEDFSFTPYT-TYFYLKAGRKAALLKSENHSITMKMKR 598          
BLAST of ATP-dependent DNA helicase vs. Ensembl Cavefish
Match: recql5 (RecQ like helicase 5 [Source:NCBI gene;Acc:103031687])

HSP 1 Score: 311.612 bits (797), Expect = 3.610e-89
Identity = 172/427 (40.28%), Postives = 245/427 (57.38%), Query Frame = 2
Query: 1235 AFKEVFGLNGFR-RNQKEAINSTLSG-FDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEI-------RPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQ-------LFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCICDNCQN 2467
            A K  FG   FR R Q+E + + L G  D FV MPTG GKSLCYQLPA++  G+T+VISPL +LI DQV  L+EL+I A  ++  L   E  +IL  L+S+   +K+LY+TPE +V S        SL  R LL+R  +DEAHCVSQWGHDFRP Y  LG LR   P VP +ALTATA  +V+EDI   L++++   +    F R NL++++        P       ++   L  +      GIVYC +R  C++VA  + + G+  + YHAG+    R + Q  W+      ++VATI+FGMG+DK +VRFV H+++ KS+  +YQE+GRAGRDG+P++C ++YS  D  ++  LI  + S   E +   ++Q          MV++CE    CR   +   FG+   +  C       CD C N
Sbjct:    9 ALKTHFGFERFRSRQQEEVVRAVLEGERDVFVCMPTGAGKSLCYQLPALLATGITLVISPLIALIQDQVEHLKELNIPARSINSKLPAGERRQILEDLESESPVLKMLYITPE-MVASPSFQPCLSSLCSRGLLARLAVDEAHCVSQWGHDFRPDYLKLGELRARLPGVPCVALTATAPKRVQEDIARSLRLRSPLTFSTPVF-RKNLKYDVIFRDLLPDPYVHLHAFAKEA-LGGSSAEKGCGIVYCRTRESCEDVAHRLTKLGIVAKPYHAGLKAGDRTESQADWMA-GKVPVIVATISFGMGVDKANVRFVAHWNVAKSLASYYQESGRAGRDGIPSSCRIYYSRKDRDQLNFLIRKEVSRKQEKRGSEKEQDKAAIQDFEAMVNFCEQE-GCRHATISQFFGDQ--KPNCAGS----CDYCHN 424          
BLAST of ATP-dependent DNA helicase vs. Ensembl Sea Lamprey
Match: blm (BLM RecQ like helicase [Source:ZFIN;Acc:ZDB-GENE-070702-5])

HSP 1 Score: 586.26 bits (1510), Expect = 0.000e+0
Identity = 301/657 (45.81%), Postives = 425/657 (64.69%), Query Frame = 2
Query: 1193 EFSG-DFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDN-SGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEH-FDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVMEISN---------VGWGSTLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNA---TVQMPISKPRTTKDKDKVIGKEPVKEVDRLQKECYQKLVEVARKFANEAKIQNYANLFSNQTLMEMSERLPADLNDLKDITSFPQVKINKYGCNFLEVTKHYN 3118
            EF G  FPH+  M+  F+  FGL  FR+NQ EAIN+ +   D FV+MPTGGGKSLCYQLPA ++ GV++V+SPL+SLI DQV +L  LDI ATHLSGD+ + ++  I +QL  +   +KLLYVTPEK+  S +L++   +L QR+LLSRFVIDEAHCVSQWGHDFRP Y+ L  LR  FP VP++ALTATATT+V+ DIL+QL M      FIQSFNR NL++E+ PK  KK   + +  + + +   SGI+YCLSR+ECD +A  + ++GL   +YHAG++D +R Q Q+ WIN+  CK++ ATIAFGMGIDKPDVR+V+H S+PKSIEG+YQE+GRAGRDG+ A CVLFY + DV R+R++I+S+   G+ E+K+ H   L+ MV +CEN+ +CRR Q+L +FGE  FD   C       CDNC  S+     +RD+T++ K VV+++  +S          V    TLN + D + G  +A++      +  ++G GS FS+++AER F  LV +G L E++ +T  D  V++V  G +   LL+G A    +Q   S     + + +V G     +V  + ++C Q+L E+ R+  +E  I+ Y N+FS  TL +++E    D   L  I    + K+ KYG   L+V   Y 
Sbjct:   91 EFQGTSFPHSAEMLKVFRHKFGLLRFRKNQLEAINAAMFNRDCFVLMPTGGGKSLCYQLPACLSPGVSVVVSPLRSLIVDQVQRLASLDIPATHLSGDINDGDLAAIYMQLAKKEPVVKLLYVTPEKLSCSTRLLSALDNLYQRQLLSRFVIDEAHCVSQWGHDFRPDYKRLSELRRRFPTVPIMALTATATTRVQTDILHQLTMNAPLSGFIQSFNRDNLKYEVLPKKPKKVAEDCLQWIRSNYPRDSGIIYCLSRHECDTMAESLRRAGLDALSYHAGLTDKQRDQSQQCWINQRRCKVICATIAFGMGIDKPDVRYVIHASLPKSIEGYYQESGRAGRDGIVAKCVLFYGYGDVTRLRRIIHSEREGGSRESKQMHMSNLYSMVHFCENISECRRLQLLAYFGEKGFDPGFCRKNPTVACDNC--SSMQTFRLRDVTENVKTVVRFLQSLSGGRWGSRGGLVTTRYTLNMLGDPWAGTKSARVT-----SGPMFGVGSSFSRHNAERLFRKLVLDGVLHEELHITGNDQAVSYVGVGLRGNDLLAGTAFKVELQETESCSSMRRSRAQVCGGSSTAQV--MAEQCAQELTELCRRLGDERGIKYY-NIFSTVTLRKIAESFSDDAEALLQIEGVTEDKMQKYGTEVLQVMSKYT 737          
BLAST of ATP-dependent DNA helicase vs. Ensembl Sea Lamprey
Match: recql (RecQ helicase-like [Source:ZFIN;Acc:ZDB-GENE-050809-134])

HSP 1 Score: 380.563 bits (976), Expect = 3.965e-119
Identity = 202/536 (37.69%), Postives = 318/536 (59.33%), Query Frame = 2
Query: 1205 DFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEI--RPKTAKKCISEIINLVNAKFRDQS--GIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCICDNCQNS--NDNKVVMRDLTDHAKAVVKYVMEISNVGWGSTLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLL 2794
            DF  + ++    +EVF ++ FR  Q +A+N++++G D F+IMPTGGGKSLCYQLPA+ ++G T+VISPL SL+ DQ+  L++L I A  LS    +  V  +   + S   ++KLLYVTPEK+ +S+  M+  +   + + L+R  IDE HC SQWG+DFRP Y++LG+L+  FP VP++ LTATAT+ V +D+   L +      F  SFNR NL +++  +P   + C+  ++ L+N K++ QS  GIVYC SR + +EVA+ +   G+   +YH+ +   +R ++   W + N  ++VVAT+AFGMGIDKPDVRFV+H+S+ KS+E +YQE+GRAGRDG PA CVL+YS+ DV R  +++  + +G        +++L+ MV+YC+    CRR+ +  HF E +D   C      +CD+C+     +   V+R    HA+ ++  +          T   +L+ ++G    K+         L       S+   E     L+  GY+ E+   T     ++++V G K +LL
Sbjct:   78 DFSWSTKLKCILREVFKMDNFRSLQLQAMNASIAGHDVFLIMPTGGGKSLCYQLPALCSKGFTLVISPLISLMEDQIMALEQLKIPAAMLSASSTKEHVKTVQAAMISNDHELKLLYVTPEKIAKSKIFMSKLEKAYKGERLARIAIDEVHCCSQWGNDFRPDYKVLGILKRQFPKVPLLCLTATATSVVLDDVKSILAVPGALS-FTASFNRPNLHYQVHRKPTKFEDCMENMVRLINKKYKGQSGPGIVYCFSRKDAEEVAAALTSLGVGAGSYHSDIPADRRSRLHRHWTS-NRLQVVVATVAFGMGIDKPDVRFVIHHSLSKSMENYYQESGRAGRDGAPADCVLYYSFPDVFRQSQMVSMERTG--------QEKLYGMVAYCQTYNRCRRSLIAEHFDEVWDAAQCDR----MCDHCRGGVVAEEASVVR----HARGLIAILRHAQQKQEKLTPLRLLEAWQGRGAVKLRVSSVPTPSL-------SQQGCEIIVLQLLMLGYIREEYSYTPYA-TISYLVVGQKARLL 587          
BLAST of ATP-dependent DNA helicase vs. Ensembl Sea Lamprey
Match: recql5 (RecQ helicase-like 5 [Source:ZFIN;Acc:ZDB-GENE-071214-1])

HSP 1 Score: 303.523 bits (776), Expect = 1.301e-90
Identity = 178/439 (40.55%), Postives = 252/439 (57.40%), Query Frame = 2
Query: 1247 VFGLNGFRRN-QKEAINSTLSGF-DTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKK---------CISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLI-------YSDNSGTI-ETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGC--ICDNCQNSNDNKVVMRDL 2500
            VFG   FR   Q++A  +  SG  D FV MPTG GKSLCYQLPAV+  GV++VI PL +LI DQ+  L +L+I A  L+  +   E   ++  L +    +K+LY+TPE +  +     V   L  R+LLS  V+DEAHCVSQWGHDFRP Y  LG LR+    VP +ALTATAT +V+EDI+  L + +    F  S  R NL + +  K   +         C+  +     A   D SGIVY  +R  CD +A  + + G+K +AYHAG+    R +VQ  W+ E    ++VATI+FGMG+DK +VRFV H++I KS+ G+YQE+GRAGRDG PA C L+Y   +  ++  LI       + D++G + ET K        +V++CE L  CR   +  +FG+         +  C   CD+C+   D+ +V++DL
Sbjct:    8 VFGFEAFRSALQEDAARAVASGTKDVFVSMPTGAGKSLCYQLPAVMAPGVSLVICPLIALIQDQLDHLHQLNIPARTLNSKMGAHERQAVVEDLHADPPSLKMLYITPE-MAATHSFGAVLGRLASRQLLSCLVVDEAHCVSQWGHDFRPDYLKLGRLRSLLRGVPCVALTATATKQVQEDIVSILSL-DKPAIFKSSCFRPNLFYNVSYKEVIQDPYKDLTQFCLKALGKKNAAGGFDGSGIVYSRTRESCDMLAHELTKRGVKTKAYHAGLKTDDRSKVQVEWM-EGETPVIVATISFGMGVDKANVRFVAHWNIAKSMAGYYQESGRAGRDGKPAFCRLYYCRKERGQVSFLISKDIAAMFQDDTGKVSETDKAAMAGFEALVNFCEKL-GCRHKAIAQYFGDK--------IADCRSACDSCK---DSALVVQDL 431          
BLAST of ATP-dependent DNA helicase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005409.1 (pep scaffold:Pmarinus_7.0:GL485130:385:11513:1 gene:ENSPMAG00000004903.1 transcript:ENSPMAT00000005409.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 106.686 bits (265), Expect = 7.173e-24
Identity = 59/134 (44.03%), Postives = 79/134 (58.96%), Query Frame = 2
Query: 2012 QAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFY--SWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCR-RTQVLNHFG 2404
            ++YHAGM+  +RK VQ  ++     +IVVAT+AFGMG+DK DVR +VHY++PKS E + QE GRAGRDG PA C +F      D+  +R+ IY++    I  KK    Q       C  L  CR R +    FG
Sbjct:  140 ESYHAGMTAAERKAVQTRFMR-GALRIVVATVAFGMGLDKADVRAIVHYNMPKSFESYVQEIGRAGRDGEPAHCHMFLDSEARDLQELRRHIYANTIDRITVKKL--LQKVFPACKCRELHLCRQRLETEEEFG 270          
BLAST of ATP-dependent DNA helicase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003898.1 (pep scaffold:Pmarinus_7.0:GL481305:13824:21767:-1 gene:ENSPMAG00000003524.1 transcript:ENSPMAT00000003898.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 61.2326 bits (147), Expect = 1.063e-9
Identity = 34/131 (25.95%), Postives = 63/131 (48.09%), Query Frame = 2
Query: 1892 SEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIY 2284
            S +++L+NA  RD   +V+  ++   D +   +   G  C + H   S   R++   ++       I+VAT     G+D P+V+ V+++ +P  IE +    GR GR G       FY+  +    R+L++
Sbjct:  311 SFLLDLLNATGRDSLTLVFVETKKGADALQGFLCSEGYSCTSIHGDRSQRDREEALSLF-RTGRRPILVATAVAARGLDIPNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGQATSFYNEKNENMTRELLH 440          
BLAST of ATP-dependent DNA helicase vs. Ensembl Yeast
Match: SGS1 (RecQ family nucleolar DNA helicase; role in genome integrity maintenance, chromosome synapsis, meiotic joint molecule/crossover formation; stimulates activity of Top3p; rapidly lost in response to rapamycin in Rrd1p-dependent manner; forms nuclear foci upon DNA replication stress; yeast SGS1 complements mutations in human homolog BLM implicated in Bloom syndrome; also similar to human WRN implicated in Werner syndrome; human BLM and WRN can each complement yeast null mutant [Source:SGD;Acc:S000004802])

HSP 1 Score: 455.677 bits (1171), Expect = 5.171e-139
Identity = 238/558 (42.65%), Postives = 348/558 (62.37%), Query Frame = 2
Query: 1208 FPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVI----TEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQ--IKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVMEISNVGWGSTLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQM------PISKPRTT 2845
            +P ++ ++    EVF L GFR NQ EA+N+TL G D FV+MPTGGGKSLCYQLPAV+    T G TIVISPL SL+ DQV  L   +I A+  S     AE  +    L   F+   + L+Y++PE +  S +       L     L+R V+DEAHCVS WGHDFRP Y+ L   +  +P++PMIALTATA+ +VR DI++ L++K    +  QSFNR NL +E+  KT K  I EI + V ++F++Q+GI+YC S+  C++ ++ M ++G+KC  YHAGM   +R  VQ+ W   +  +++ AT+AFGMGIDKPDVRFV H+++P+++EG+YQETGRAGRDG  + C+ ++S+ D+  M+ +I  D +   E K++H  +L  +++YC+N+ DCRR  VL++F E FD KLC       CDNC+NS +     RD+T+ AK +VK V  I N     T+ Y  DVF+G+ ++KIV+        +G G    K++ ER F HL+    L E  ++  + F  ++V  GP  + LL+G   ++M      P S+P T+
Sbjct:  660 YPWSDEVLYRLHEVFKLPGFRPNQLEAVNATLQGKDVFVLMPTGGGKSLCYQLPAVVKSGKTHGTTIVISPLISLMQDQVEHLLNKNIKASMFSS-RGTAEQRRQTFNL---FINGLLDLVYISPEMISASEQCKRAISRLYADGKLARIVVDEAHCVSNWGHDFRPDYKELKFFKREYPDIPMIALTATASEQVRMDIIHNLELKEPV-FLKQSFNRTNLYYEVNKKT-KNTIFEICDAVKSRFKNQTGIIYCHSKKSCEQTSAQMQRNGIKCAYYHAGMEPDERLSVQKAW-QADEIQVICATVAFGMGIDKPDVRFVYHFTVPRTLEGYYQETGRAGRDGNYSYCITYFSFRDIRTMQTMIQKDKNLDRENKEKHLNKLQQVMAYCDNVTDCRRKLVLSYFNEDFDSKLCHKN----CDNCRNSANVINEERDVTEPAKKIVKLVESIQN--ERVTIIYCQDVFKGSRSSKIVQANHDTLEEHGIGKSMQKSEIERIFFHLITIRVLQEYSIMNNSGFASSYVKVGPNAKKLLTGKMEIKMQFTISAPNSRPSTS 1204          
BLAST of ATP-dependent DNA helicase vs. Ensembl Yeast
Match: HRQ1 (3'-5' DNA helicase that has DNA strand annealing activity; helicase activity is stimulated by fork structure and 3'-tail length of substrates; acts with Rad4p in nucleotide-excision repair; belongs to the widely conserved RecQ family of proteins which are involved in maintaining genomic integrity; similar to the human RecQ4p implicated in Rothmund-Thomson syndrome (RTS) [Source:SGD;Acc:S000002699])

HSP 1 Score: 60.4622 bits (145), Expect = 1.519e-9
Identity = 86/350 (24.57%), Postives = 144/350 (41.14%), Query Frame = 2
Query: 1274 NQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVI-----TEGVTIVISPLKSLIHDQ-------VTKLQEL-DIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRF---VIDEAHCVSQW--GHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLR----WE--IRPKTAKKCISEIIN----LVNAKFRDQSGIVYCLSRNECD----EVASVMAQSG-----LKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGR 2212
            +Q +AINS   G +  +   T  GKSL YQL A+       E   + I P K+L  DQ       ++K+ EL + V     GD +  E   I           ++++  P+ ++ +  L N     N R  L      V+DE H        H      RLL L    + N  +  ++ +AT K     +  +   N      +  +    +    W   I P+  +K  + I      LV     +   I +C  R  C+    EV ++  ++G      +  +Y  G S + R++++    + N  K V++T A  +GID   +  V+    P S+  F+Q++GRAGR
Sbjct:  294 HQADAINSLHQGENVIITTSTSSGKSLIYQLAAIDLLLKDPESTFMYIFPTKALAQDQKRAFKVILSKIPELKNAVVDTYDGDTEPEERAYIR-------KNARVIFTNPD-MIHTSILPN---HANWRHFLYHLKLVVVDELHIYKGLFGSHVALVMRRLLRLCHCFYENSGLQFISCSATLKSPVQHMKDMFGINEVTLIHEDGSPTGAKHLVVWNPPILPQHERKRENFIRESAKILVQLILNNVRTIAFCYVRRVCELLMKEVRNIFIETGREDLVTEVMSYRGGYSASDRRKIEREMFHGN-LKAVISTNALELGIDIGGLDAVLMCGFPLSMANFHQQSGRAGR 631          
BLAST of ATP-dependent DNA helicase vs. Ensembl Yeast
Match: DHH1 (Cytoplasmic DEAD-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacting with both decapping and deadenylase complexes; role in translational repression, mRNA decay, and possibly mRNA export; interacts and cooperates with Ngr1p to promote specific mRNA decay; ATP- and RNA-bound form promotes processing body (PB) assembly, while ATPase stimulation by Not1p promotes PB disassembly; forms cytoplasmic foci on replication stress [Source:SGD;Acc:S000002319])

HSP 1 Score: 58.5362 bits (140), Expect = 3.591e-9
Identity = 75/356 (21.07%), Postives = 146/356 (41.01%), Query Frame = 2
Query: 1277 QKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDL-KEAEVDKILL----QLQSQFMQ----IKLLYVTPEKVVE--SRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFR-----------PHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKL 2278
            Q+EAI   ++G D       G GK+  + +P +  E V   ++ +++LI   +   +EL +  + +   L K   +  ++      L+   ++    + +L  TP +V++  SRK+ ++          S F++DEA        DF+           P ++ L L   TFP      +          +++ +L +K  T+++     R  L                +N + +K +    I++C S N  + +A  +   G  C   HA M   +R +V   +  +   + +V +     GID   V  V+++  PK+ E +    GR+GR G     +   +W D   + K+
Sbjct:   73 QEEAIPVAITGRDILARAKNGTGKTAAFVIPTL--EKVKPKLNKIQALI---MVPTRELALQTSQVVRTLGKHCGISCMVTTGGTNLRDDILRLNETVHILVGTPGRVLDLASRKVADLSDC-------SLFIMDEAD--KMLSRDFKTIIEQILSFLPPTHQSL-LFSATFPLTVKEFMVKHLHKPYEINLMEELTLKGITQYYAFVEERQKLHC--------------LNTLFSKLQINQAIIFCNSTNRVELLAKKITDLGYSCYYSHARMKQQERNKVFHEF-RQGKVRTLVCSDLLTRGIDIQAVNVVINFDFPKTAETYLHRIGRSGRFGHLGLAINLINWNDRFNLYKI 398          
BLAST of ATP-dependent DNA helicase vs. Ensembl Yeast
Match: FAL1 (Nucleolar protein required for maturation of 18S rRNA; member of the eIF4A subfamily of DEAD-box ATP-dependent RNA helicases; 18S rRNA biogenesis defect of the null mutant is functionally complemented by human EIF4A3 [Source:SGD;Acc:S000002428])

HSP 1 Score: 57.3806 bits (137), Expect = 6.344e-9
Identity = 26/113 (23.01%), Postives = 58/113 (51.33%), Query Frame = 2
Query: 1940 IVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKL 2278
            +++C ++ + D ++  + QS     + H  M   +R +V   +      +++++T  +  GID   V  V++Y +P+ IE +    GR+GR G     + F +  D+ ++R++
Sbjct:  269 VIFCNTKKKVDWLSQRLIQSNFAVVSMHGDMKQEERDKVMNDF-RTGHSRVLISTDVWARGIDVQQVSLVINYDLPEIIENYIHRIGRSGRFGRKGVAINFITKADLAKLREI 380          
BLAST of ATP-dependent DNA helicase vs. Ensembl Yeast
Match: DBP3 (RNA-Dependent ATPase, member of DExD/H-box family; involved in cleavage of site A3 within the ITS1 spacer during rRNA processing; not essential for growth, but deletion causes severe slow-growth phenotype [Source:SGD;Acc:S000003046])

HSP 1 Score: 56.9954 bits (136), Expect = 1.319e-8
Identity = 81/346 (23.41%), Postives = 146/346 (42.20%), Query Frame = 2
Query: 1301 LSGFDTFVIMPTGGGKSLCYQLPAVI-------TEGVTI-VISP---LKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWG--HDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQ----------SG-------IVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYS 2248
            LSG D   +  TG GK+  + +PA+          G+ + VISP   L S I+D +  L   D V             D+  +QL+      +++  TP ++++  +  +V   L+Q   ++  V+DEA  + + G   D +   R      T       +  TAT   +VRE  L    M N  K  I + ++          TA K I++I+ +V+ + +++          SG       +++ L + E   V   +  +G    A H  +S  +R Q    +       +++AT     G+D P+V+ V++ + P ++E +    GR GR G   T    ++
Sbjct:  147 LSGKDVVGVAETGSGKTFAFGVPAISHLMNDQKKRGIQVLVISPTRELASQIYDNLIVLT--DKVGMQCCCVYGGVPKDEQRIQLKKS----QVVVATPGRLLDLLQEGSV--DLSQ---VNYLVLDEADRMLEKGFEEDIKNIIR-----ETDASKRQTLMFTATWPKEVRE--LASTFMNNPIKVSIGNTDQ---------LTANKRITQIVEVVDPRGKERKLLELLKKYHSGPKKNEKVLIFALYKKEAARVERNLKYNGYNVAAIHGDLSQQQRTQALNEF-KSGKSNLLLATDVAARGLDIPNVKTVINLTFPLTVEDYVHRIGRTGRAGQTGTAHTLFT 464          
BLAST of ATP-dependent DNA helicase vs. Ensembl Nematostella
Match: EDO32632 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SUG5])

HSP 1 Score: 390.193 bits (1001), Expect = 1.072e-122
Identity = 200/478 (41.84%), Postives = 293/478 (61.30%), Query Frame = 2
Query: 1199 SGDFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEI--RPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCICDNCQNSNDNKVV--MRDLTDHAKAVVKYVMEISNVGWGSTLNYVLDVF--RGANNAKI 2614
            S  F  ++ +  A K VF ++ FR  Q E IN+T+SG D  +IMPTGGGKSLC+QLPAV+++G+T+V+SPL SL+ DQ+  L+ L I A  L+      EV+ +   +  +   +K+LYVTPEK+ +S++ M   +   +  LLSR VIDE HC SQWGHDFRP Y++LG+L+  +P VP++ LTATATTKV ED+   L +  N      SFNR NL +E+  +P T    +S I  L+  +F   SGI+YC SR + ++VA  M+  G+K   YHA M    R QV   W   N  ++VVAT+AFGMGIDK +VRFV+H+S  KS+E +YQE+GRAGRD   A+C++FY   D+ R   +++++ +G         Q L+ +V YC+    CRRT +  HFGE +D   C      +CDNC   +D + +  + D+T +A  ++K + +    G   T N ++D +  RGA + ++
Sbjct:   68 STAFSWSQEVEAALKNVFKIDTFRHLQLECINATMSGVDCILIMPTGGGKSLCFQLPAVVSKGLTLVVSPLVSLMEDQLWALKRLGIKAALLNASSTREEVNSVHASIVDKKSDLKMLYVTPEKIAKSKRFMAKLEKSYESGLLSRIVIDEVHCTSQWGHDFRPDYKILGILKRQYPGVPILGLTATATTKVIEDVKKILGLHANCLLLKASFNRPNLFYEVQSKPTTNSAFMSTIHQLITKRFSGDSGIIYCFSRKDAEQVAIEMSSRGIKAACYHADMPPESRSQVHMAWTT-NKLQVVVATVAFGMGIDKSNVRFVIHHSFSKSMENYYQESGRAGRDEKRASCIVFYRPFDIFRHSTMVFTEQTGL--------QNLYGIVRYCQQQGVCRRTLIGRHFGEGWDPAQCKQ----MCDNCSRRDDERGLNELHDITQYASDLLKILEQAKAAGDRVTANKLVDAWCGRGAVSLRV 532          
BLAST of ATP-dependent DNA helicase vs. Ensembl Nematostella
Match: EDO34091 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SQC9])

HSP 1 Score: 325.094 bits (832), Expect = 1.507e-100
Identity = 181/425 (42.59%), Postives = 253/425 (59.53%), Query Frame = 2
Query: 1238 FKEVFGLNGFRRN-QKEAINSTLSGF-DTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPK-TAKKCISEIINLVNAKFRDQ-------SGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSD------NSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCICDNCQ 2464
             + VF    F+   Q+ A  +   G  D FV MPTG GKSLCYQLPAV+  G+TIV SPL +LI DQVT L+ L I    L+  L E+E  +++  L      +KLLY+TPE +  +     V  SL +RKLLS F +DEAHCVSQWGHDFRP Y  LG LR  + +V  +ALTATAT++V+EDIL  L M++N   F     R NL ++I  K   +   +++    +A   D+       SGI+YC +R+ C EVAS +++ GL  + YHAG+   KR +VQ+ W+ E    ++ ATI+FGMG+DK DVRFV H+S+P+S+EG+YQE+GRAGRDG P+ C L+YS  +  ++  LI +D       +   +  K  +     +V YCE    CR  ++  +FG+   +  C   KG  CD C+
Sbjct:    9 LRNVFKFREFKSELQQRACETVSKGLQDVFVSMPTGSGKSLCYQLPAVVAPGITIVFSPLIALIQDQVTYLRTLKITVETLNSKLPESERKRVMKDLYFVKPTVKLLYITPE-LAATPGFQKVLDSLYKRKLLSLFAVDEAHCVSQWGHDFRPDYLRLGKLRNKYKDVAWVALTATATSRVKEDILTALHMQSNVAVFKSQCYRPNLYYDISFKELLEDPYADLKQFADAALEDEDSGEAKGSGIIYCRTRDACQEVASRLSRKGLSAKPYHAGLKSDKRDKVQQEWM-EGKVAVICATISFGMGVDKGDVRFVAHWSLPQSMEGYYQESGRAGRDGQPSYCRLYYSRAERDQVLFLIKNDMKKRLKKANAAQKNKAVQVSFQAVVEYCEE-PSCRHGRIAKYFGDEMPQ--CN--KG--CDYCK 424          
BLAST of ATP-dependent DNA helicase vs. Ensembl Nematostella
Match: EDO25520 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A8DW89])

HSP 1 Score: 205.297 bits (521), Expect = 2.658e-58
Identity = 110/287 (38.33%), Postives = 173/287 (60.28%), Query Frame = 2
Query: 1421 IHDQVTKLQELDIVATHLSGDLKEAEVDKILLQ-LQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQ-SGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRK 2275
            + DQV  L++L+I A ++   L   +V +IL   L   +   +LLY++PE++ ++R       +LN    ++   +DEAHC+SQWGHDFRP Y  +  L+  F   P++A+TA+AT +V++DI  QL++K  T  + +   R N+ + I     K       +L+ A   ++ S I+YC SR   + + +++ Q+G     YHAG+    R+  Q  W+N N  + +VAT AFGMGIDKPDVR VV+Y +P+ +E +YQE+GRAGRD LPA  +L Y+ TD+ R+ +
Sbjct:    1 MKDQVATLKKLNISAEYIHSGLHYNDVKRILNNALHGGY---ELLYISPERL-QTRLFEEYLPALN----INLIAVDEAHCISQWGHDFRPDYLKIAQLKQVFARTPVLAVTASATNEVKQDIARQLQLKTPT-LYQKGIERKNIHYTIHYSERKDN-----DLLEALGNNKGSKIIYCRSRKTTERINNLLLQNGYSSVVYHAGLDKKVRENAQNDWLN-NNTETIVATTAFGMGIDKPDVRLVVNYDLPEHLEAYYQESGRAGRDELPAKSILLYNQTDINRLEQ 272          
BLAST of ATP-dependent DNA helicase vs. Ensembl Nematostella
Match: EDO32537 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SUT5])

HSP 1 Score: 193.741 bits (491), Expect = 1.933e-51
Identity = 123/364 (33.79%), Postives = 197/364 (54.12%), Query Frame = 2
Query: 1301 LSGFDTFVIMPTGGGKSLCYQLPAVI----TEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMN-VFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHY-RLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEI-RPKTAKKCISEIINLVNAKF-RDQSGIVYCLSRNECDEVASVMAQ---------------SGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFY--SWTDVVRMRKLIYSDNSGTIETKK 2317
            LSG  + V++ TG GKSLCYQLPA +    +  +T+VISPL SL+ DQ+T L    +    L  +  + +  KIL  + +   ++ +L V+PE +V      +    S  +   ++   IDEAHC+S+W H+FRP Y R+  +LR  +     + LTATAT    + +   L + +       +    NLR  + R +   K + E++     +F R +S I+YC  R   + VA+++                 SG   ++YHAGMS  +R++VQ+ +++    + V AT+AFGMG+DK DVR ++HY++PKS E + QE GRAGRDG P+ C +F      D+  +R+ I+S+    +  K+
Sbjct:    4 LSGMSSLVVLSTGAGKSLCYQLPAYMYHKRSPCLTLVISPLVSLMEDQITGL-PFGLKGACLHNNQTKPQRLKILTDITAG--KVAVLLVSPEALVGGGMGGSGCLPSSTKMPPIAFACIDEAHCLSEWSHNFRPSYLRVCKILRDRYGVQCFLGLTATATQSTADSVAQHLGIADAGAVIRGAAVPPNLRLSVSRDRDKDKALIELLQ--GPRFSRCESIIIYCTRREVTERVATLIRTCMQHMQPTIRHDDVISGSDAESYHAGMSAAQRRKVQKRFMS-GELRAVAATVAFGMGLDKSDVRAIIHYNMPKSFESYVQEIGRAGRDGKPSHCHVFLDREGQDLCELRRHIFSNTVDRVTVKR 361          
BLAST of ATP-dependent DNA helicase vs. Ensembl Nematostella
Match: EDO46405 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RPZ5])

HSP 1 Score: 162.54 bits (410), Expect = 5.056e-42
Identity = 125/422 (29.62%), Postives = 201/422 (47.63%), Query Frame = 2
Query: 1256 LNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVI----------TEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWG---HDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEI---RPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAY---HAGMSDTKRKQVQEMWINENG-CKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCICDNC 2461
            +   + +Q +A+ S L+  DTF  +PTG GKSL YQ+  ++          T+   +VISPL +LI DQ+   + L ++A     DL + E  K + + +  F   ++L     + +E+++L++    L+ R L    V+DE+HCV+ WG     FR  Y  +G LR  F   P++ LTATA   VR  I+  L M+N     +    +          R    +   + II  ++   + +  I+YC S N C ++  V++++    + Y   H+   D+ +K+V   +I  N   ++VVAT A GMG+D PDV  ++H+ IP  +E + QE GR GRDG      L+Y      +   L + D+   +    + EQ              CRR  +  HF E    K    +  C CDNC
Sbjct:   27 IKSLKDSQFDALYSFLNSEDTFACLPTGYGKSLIYQMAVMVARSAKISGLPTKPFVVVISPLNALIQDQIDSCRSLGLIAAKFELDLCKEENSKTVDETEVVFCSPEML-----EPLEAKRLLS---KLDHRLL--GIVVDESHCVTSWGLTVTPFRAAYGKIGDLRG-FTKKPIMCLTATAGKTVRNKIIKNLHMQNARVINLAPDKKNIKLNIKKVGRDVELENIFTTIIKKLSNGGQVEKTIIYCKSINACGDIYEVLSENVESKETYAMFHSKTPDSIQKEVLGEFIKSNSKIRVVVATCALGMGVDIPDVDHIIHFGIPSEVEHYVQEIGRGGRDGRLCYATLYY------KPYHLAHCDSG--MRNYAKGEQ--------------CRREMLAKHFKEKI--KGVQPLHNC-CDNC 412          
BLAST of ATP-dependent DNA helicase vs. Ensembl Medaka
Match: blm (BLM RecQ like helicase [Source:NCBI gene;Acc:100049173])

HSP 1 Score: 585.489 bits (1508), Expect = 0.000e+0
Identity = 314/713 (44.04%), Postives = 451/713 (63.25%), Query Frame = 2
Query: 1196 FSG-DFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGE-HFDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVME-ISNVG--WGS-------TLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISKPRTTKDKDKVIGKEPVKEVDRLQKECYQKLVEVARKFANEAKIQNYANLFSNQTLMEMSERLPADLNDLKDITSFPQVKINKYGCNFLEVTKHYN--LLLTKHKQEMK-----DLEKKLTVDIKPENAKSSYFSSTS-------KAPAKGYNRKR 3256
            F G +FPH++ MM  F + FGL+ FR NQ EAIN+ L G D FV+MPTGGGKSLCYQLPA ++ GVT+V+SPLKSLI DQ+ KL  LDI AT LSGD  ++E  +I +QL  +   IKLLYVTPEKV  S +L++  Q+L +R LL+RF+IDEAHCVSQWGHDFRP ++ L  LR  FP+V M+ALTATAT +V++DIL QL M    + F  SFNR NL++ + PK  KK   + I  +   +   SGIVYCLSRN+CD +A  + ++G++  +YHAG+SD  R+ VQ  WIN++GC+++ ATIAFGMGIDKPDVR+V+H S+PKS+EG+YQE+GRAGRDG  + C+LFYS+TDV R++++I  D  G   TK  H   L  MV +CEN+++CRR Q+L +FGE +F+R  C       CDNC  +  N+  M+++T+  K +V++V E    VG  +G        TLN ++D+F G+ +AKI         ++G G  +S+++A+R F  LV E  L E + +T     V+++  GPK   +LSG   V    ++  ++  K K    + V + D + + C ++LV++ ++      + +Y N+FS  TL +++ERL +D   L  I    + K+ KYG   ++V + Y+   L  + + E       D  +  T D   E+  S+YFS+ S       KAP   Y++KR
Sbjct:  593 FRGFNFPHSQEMMKIFHKRFGLHQFRFNQLEAINAALLGEDAFVLMPTGGGKSLCYQLPACVSPGVTVVVSPLKSLIVDQIQKLTTLDIPATSLSGDKSDSEAGRIYMQLSRKDPLIKLLYVTPEKVSASGRLISALQNLYERGLLARFIIDEAHCVSQWGHDFRPDFKRLHELRQKFPSVRMMALTATATPRVQKDILNQLNMM-RPQVFTMSFNRSNLKYSVLPKKPKKVDEDCIGWIKKHYPRDSGIVYCLSRNDCDAMAESLKRAGIQALSYHAGLSDGDREYVQSKWINQDGCQVICATIAFGMGIDKPDVRYVIHASLPKSVEGYYQESGRAGRDGEISHCILFYSYTDVHRIKRIISMDREGDSHTKATHFNNLHSMVHFCENVMECRRIQLLAYFGELNFNRNFCKDHPDVSCDNC--AKPNQYQMKNVTEDVKKIVRFVQENCEKVGSRFGKTAQQNRLTLNMLVDIFIGSKSAKI------QTGMFGMGGAYSRHNADRLFKKLVLENILVEDLYITNNGQAVSYISAGPKAMNVLSGYMQVDFYETESASSIRKQKAAVSKSVSQRDEMVQACLKELVDLCKELGKAFGL-HYYNIFSTATLKKIAERLSSDPEVLLQIDGVTEDKLEKYGAEVIKVLQKYSERQLPVEEQAEGAGEGWIDTTRGRTQDEDDES--STYFSNRSAQGQKRKKAPFFKYSKKR 1293          
BLAST of ATP-dependent DNA helicase vs. Ensembl Medaka
Match: blm (BLM RecQ like helicase [Source:NCBI gene;Acc:100049173])

HSP 1 Score: 585.489 bits (1508), Expect = 0.000e+0
Identity = 314/713 (44.04%), Postives = 451/713 (63.25%), Query Frame = 2
Query: 1196 FSG-DFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGE-HFDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVME-ISNVG--WGS-------TLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISKPRTTKDKDKVIGKEPVKEVDRLQKECYQKLVEVARKFANEAKIQNYANLFSNQTLMEMSERLPADLNDLKDITSFPQVKINKYGCNFLEVTKHYN--LLLTKHKQEMK-----DLEKKLTVDIKPENAKSSYFSSTS-------KAPAKGYNRKR 3256
            F G +FPH++ MM  F + FGL+ FR NQ EAIN+ L G D FV+MPTGGGKSLCYQLPA ++ GVT+V+SPLKSLI DQ+ KL  LDI AT LSGD  ++E  +I +QL  +   IKLLYVTPEKV  S +L++  Q+L +R LL+RF+IDEAHCVSQWGHDFRP ++ L  LR  FP+V M+ALTATAT +V++DIL QL M    + F  SFNR NL++ + PK  KK   + I  +   +   SGIVYCLSRN+CD +A  + ++G++  +YHAG+SD  R+ VQ  WIN++GC+++ ATIAFGMGIDKPDVR+V+H S+PKS+EG+YQE+GRAGRDG  + C+LFYS+TDV R++++I  D  G   TK  H   L  MV +CEN+++CRR Q+L +FGE +F+R  C       CDNC  +  N+  M+++T+  K +V++V E    VG  +G        TLN ++D+F G+ +AKI         ++G G  +S+++A+R F  LV E  L E + +T     V+++  GPK   +LSG   V    ++  ++  K K    + V + D + + C ++LV++ ++      + +Y N+FS  TL +++ERL +D   L  I    + K+ KYG   ++V + Y+   L  + + E       D  +  T D   E+  S+YFS+ S       KAP   Y++KR
Sbjct:  623 FRGFNFPHSQEMMKIFHKRFGLHQFRFNQLEAINAALLGEDAFVLMPTGGGKSLCYQLPACVSPGVTVVVSPLKSLIVDQIQKLTTLDIPATSLSGDKSDSEAGRIYMQLSRKDPLIKLLYVTPEKVSASGRLISALQNLYERGLLARFIIDEAHCVSQWGHDFRPDFKRLHELRQKFPSVRMMALTATATPRVQKDILNQLNMM-RPQVFTMSFNRSNLKYSVLPKKPKKVDEDCIGWIKKHYPRDSGIVYCLSRNDCDAMAESLKRAGIQALSYHAGLSDGDREYVQSKWINQDGCQVICATIAFGMGIDKPDVRYVIHASLPKSVEGYYQESGRAGRDGEISHCILFYSYTDVHRIKRIISMDREGDSHTKATHFNNLHSMVHFCENVMECRRIQLLAYFGELNFNRNFCKDHPDVSCDNC--AKPNQYQMKNVTEDVKKIVRFVQENCEKVGSRFGKTAQQNRLTLNMLVDIFIGSKSAKI------QTGMFGMGGAYSRHNADRLFKKLVLENILVEDLYITNNGQAVSYISAGPKAMNVLSGYMQVDFYETESASSIRKQKAAVSKSVSQRDEMVQACLKELVDLCKELGKAFGL-HYYNIFSTATLKKIAERLSSDPEVLLQIDGVTEDKLEKYGAEVIKVLQKYSERQLPVEEQAEGAGEGWIDTTRGRTQDEDDES--STYFSNRSAQGQKRKKAPFFKYSKKR 1323          
BLAST of ATP-dependent DNA helicase vs. Ensembl Medaka
Match: recql (RecQ like helicase [Source:NCBI gene;Acc:101164117])

HSP 1 Score: 368.237 bits (944), Expect = 1.620e-111
Identity = 216/607 (35.58%), Postives = 332/607 (54.70%), Query Frame = 2
Query: 1202 GDFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAK--FRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVMEISNVGWGSTLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISKPRTTKDKDKVIGKEPVKEVDRLQKECYQKLVEVARKFANEAKIQNYANLFSNQTLMEMSER 3016
             +FP +  +    +  F L+ FR  Q  AIN T+ G D F++MPTG GKSLCYQLPAV +EG T+V+SPL SL+ DQ+  L+ L + A  L+    +     +L  L       KL+YVTPEK+ +S+ LM+  +   + KLLSR  +DE HC SQWGHDFRP Y+LLG+L+  FP+VP++ LTATAT+ V +D    L +         SFNR NL +E+R K +   +S        K  +++QSGIVY  S+ + + V+S + +SG++   YHA M    + +V   W + N  ++VVAT+AFGMGIDKPDVRFV+H++I KSIE +YQE+GRAGRD  PA C++++ + D+ R+  ++  ++ G        +++L  MV +C+N+  CRR+ +  HF E +D + C      +CD C++      +  D+T HA+ VV+ V   +++    T   +++ +RG   AK          +  T +   +  AE     L+ +GYL E    T       +V  G +  LL +   ++ M          K K+ G EPV ++   +        EV    A +  ++   N+   Q L   S++
Sbjct:   84 AEFPWSAAVDQHLRTSFHLSAFRPLQLRAINLTMEGRDVFLVMPTGRGKSLCYQLPAVCSEGFTLVVSPLVSLMEDQLLLLRSLQVPAAALNASSGKEHTKTVLAALTDPKAPFKLVYVTPEKIAKSKLLMSRLEKAYKAKLLSRMAVDEVHCCSQWGHDFRPDYKLLGILKRQFPSVPLLGLTATATSSVLQDCRKILCVPQPVT-LTASFNRANLYYEVRVKDSDSDLSLSDISSLIKNRYKEQSGIVYVFSQKDAESVSSALQKSGIQAYPYHANMDPEDKSRVHRKWTS-NKIQVVVATVAFGMGIDKPDVRFVIHHTISKSIENYYQESGRAGRDDRPADCIVYFGFADIFRISTMVVMESVG--------QKKLLQMVDFCQNVDRCRRSLMAVHFDEVWDDEDCNQ----MCDTCRHPAGYNTL--DVTQHARQVVQIVELATSMDERLTPLKLVEAWRGRGPAK-------RRKMIQT-TELPRRQAEAVIVFLLLQGYLREDFSFTPYTTYF-YVKLGRRAPLLKNQAHSLTM----------KMKMSGAEPVSDLSAAESS------EVKGPHAKQKVLKEQMNMTRKQDLQPSSQK 649          
BLAST of ATP-dependent DNA helicase vs. Ensembl Medaka
Match: recql (RecQ like helicase [Source:NCBI gene;Acc:101164117])

HSP 1 Score: 368.237 bits (944), Expect = 1.620e-111
Identity = 216/607 (35.58%), Postives = 332/607 (54.70%), Query Frame = 2
Query: 1202 GDFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAK--FRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVMEISNVGWGSTLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISKPRTTKDKDKVIGKEPVKEVDRLQKECYQKLVEVARKFANEAKIQNYANLFSNQTLMEMSER 3016
             +FP +  +    +  F L+ FR  Q  AIN T+ G D F++MPTG GKSLCYQLPAV +EG T+V+SPL SL+ DQ+  L+ L + A  L+    +     +L  L       KL+YVTPEK+ +S+ LM+  +   + KLLSR  +DE HC SQWGHDFRP Y+LLG+L+  FP+VP++ LTATAT+ V +D    L +         SFNR NL +E+R K +   +S        K  +++QSGIVY  S+ + + V+S + +SG++   YHA M    + +V   W + N  ++VVAT+AFGMGIDKPDVRFV+H++I KSIE +YQE+GRAGRD  PA C++++ + D+ R+  ++  ++ G        +++L  MV +C+N+  CRR+ +  HF E +D + C      +CD C++      +  D+T HA+ VV+ V   +++    T   +++ +RG   AK          +  T +   +  AE     L+ +GYL E    T       +V  G +  LL +   ++ M          K K+ G EPV ++   +        EV    A +  ++   N+   Q L   S++
Sbjct:   84 AEFPWSAAVDQHLRTSFHLSAFRPLQLRAINLTMEGRDVFLVMPTGRGKSLCYQLPAVCSEGFTLVVSPLVSLMEDQLLLLRSLQVPAAALNASSGKEHTKTVLAALTDPKAPFKLVYVTPEKIAKSKLLMSRLEKAYKAKLLSRMAVDEVHCCSQWGHDFRPDYKLLGILKRQFPSVPLLGLTATATSSVLQDCRKILCVPQPVT-LTASFNRANLYYEVRVKDSDSDLSLSDISSLIKNRYKEQSGIVYVFSQKDAESVSSALQKSGIQAYPYHANMDPEDKSRVHRKWTS-NKIQVVVATVAFGMGIDKPDVRFVIHHTISKSIENYYQESGRAGRDDRPADCIVYFGFADIFRISTMVVMESVG--------QKKLLQMVDFCQNVDRCRRSLMAVHFDEVWDDEDCNQ----MCDTCRHPAGYNTL--DVTQHARQVVQIVELATSMDERLTPLKLVEAWRGRGPAK-------RRKMIQT-TELPRRQAEAVIVFLLLQGYLREDFSFTPYTTYF-YVKLGRRAPLLKNQAHSLTM----------KMKMSGAEPVSDLSAAESS------EVKGPHAKQKVLKEQMNMTRKQDLQPSSQK 649          
BLAST of ATP-dependent DNA helicase vs. Ensembl Medaka
Match: recql (RecQ like helicase [Source:NCBI gene;Acc:101164117])

HSP 1 Score: 368.237 bits (944), Expect = 1.620e-111
Identity = 216/607 (35.58%), Postives = 332/607 (54.70%), Query Frame = 2
Query: 1202 GDFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAK--FRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVMEISNVGWGSTLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISKPRTTKDKDKVIGKEPVKEVDRLQKECYQKLVEVARKFANEAKIQNYANLFSNQTLMEMSER 3016
             +FP +  +    +  F L+ FR  Q  AIN T+ G D F++MPTG GKSLCYQLPAV +EG T+V+SPL SL+ DQ+  L+ L + A  L+    +     +L  L       KL+YVTPEK+ +S+ LM+  +   + KLLSR  +DE HC SQWGHDFRP Y+LLG+L+  FP+VP++ LTATAT+ V +D    L +         SFNR NL +E+R K +   +S        K  +++QSGIVY  S+ + + V+S + +SG++   YHA M    + +V   W + N  ++VVAT+AFGMGIDKPDVRFV+H++I KSIE +YQE+GRAGRD  PA C++++ + D+ R+  ++  ++ G        +++L  MV +C+N+  CRR+ +  HF E +D + C      +CD C++      +  D+T HA+ VV+ V   +++    T   +++ +RG   AK          +  T +   +  AE     L+ +GYL E    T       +V  G +  LL +   ++ M          K K+ G EPV ++   +        EV    A +  ++   N+   Q L   S++
Sbjct:   84 AEFPWSAAVDQHLRTSFHLSAFRPLQLRAINLTMEGRDVFLVMPTGRGKSLCYQLPAVCSEGFTLVVSPLVSLMEDQLLLLRSLQVPAAALNASSGKEHTKTVLAALTDPKAPFKLVYVTPEKIAKSKLLMSRLEKAYKAKLLSRMAVDEVHCCSQWGHDFRPDYKLLGILKRQFPSVPLLGLTATATSSVLQDCRKILCVPQPVT-LTASFNRANLYYEVRVKDSDSDLSLSDISSLIKNRYKEQSGIVYVFSQKDAESVSSALQKSGIQAYPYHANMDPEDKSRVHRKWTS-NKIQVVVATVAFGMGIDKPDVRFVIHHTISKSIENYYQESGRAGRDDRPADCIVYFGFADIFRISTMVVMESVG--------QKKLLQMVDFCQNVDRCRRSLMAVHFDEVWDDEDCNQ----MCDTCRHPAGYNTL--DVTQHARQVVQIVELATSMDERLTPLKLVEAWRGRGPAK-------RRKMIQT-TELPRRQAEAVIVFLLLQGYLREDFSFTPYTTYF-YVKLGRRAPLLKNQAHSLTM----------KMKMSGAEPVSDLSAAESS------EVKGPHAKQKVLKEQMNMTRKQDLQPSSQK 649          
BLAST of ATP-dependent DNA helicase vs. Planmine SMEST
Match: SMESG000060148.1 (SMESG000060148.1)

HSP 1 Score: 1993.39 bits (5163), Expect = 0.000e+0
Identity = 1027/1034 (99.32%), Postives = 1031/1034 (99.71%), Query Frame = 2
Query:  281 MEINKIEIAKTLPTAKIIPLQNVSPAKQILPESSTLSLDDQDVFSDDFLVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIISQSKPVCSTPIPSAVNKLTINKIKPNETLSKNLTGVRITQKYHDLLDNFFGYLEQFPISKLVEFFDDKSSLVSEILTERNINKKAMLALSDRSIVLESSSFQSITPGNSYGDIFDKDETVVDFDEQDEICENQFEPSVKSFDEWEDGDDVFESIDESLFIADTENFVGQLNAYRNENAPMINVTNAADLDGTSYVPINEKQSKWAIEDFEYKSHPNDGETAEFSGDFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVMEISNVGWGSTLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISKPRTTKDKDKVIGKEPVKEVDRLQKECYQKLVEVARKFANEAKIQNYANLFSNQTLMEMSERLPADLNDLKDITSFPQVKINKYGCNFLEVTKHYNLLLTKHKQEMKDLEKKLTVDIKPENAKSSYFSSTSKAPAKGYNRKRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAGLMKLTVRS 3382
            MEINK EIAKTLPTAKIIPLQNVSPAKQILPESSTLSLDDQDVFSDDFLVDNPPKLLKPITK KTTLSTSKTNLSKPSKNKIISQSKPVCSTPIPSAVNKLTINKIKPNETLSKN TGVRITQKYHDLLDNFFGYLEQFPISKLVEFFDDKSSLVSEILTERNINKKAMLALSDRSIVLE+SSFQSITPGNSYGDIFDKDETVVDFDEQDEICENQFEPSVKSFDEWEDGDDVFESIDESLFIADTENFVGQLNAYRNENAPMINVTNAADLDGTSYVPINEKQSKWAIEDFEYKSHPNDGETAEFSGDFPHTERMMIAFKEVFGLNGFRRNQKEAINSTL+GFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLG+LRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGT+ETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVMEISNVGWGSTLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISKPRTTKDKDKVIGKEPVKEVDRLQKECYQKLVEVARKFANEAKIQNYANLFSNQTLMEMSERLPADLNDLKDITSFPQVKINKYGCNFLEVTKHYNLLLTKHKQEMKDLEKKLTVDIKPENAKSSYFSSTSKAPAKGYNRKRKSPNNVFSKKFSNFKRSKTASSKSESVSKKKSAGLMKLTVRS
Sbjct:    1 MEINKNEIAKTLPTAKIIPLQNVSPAKQILPESSTLSLDDQDVFSDDFLVDNPPKLLKPITKTKTTLSTSKTNLSKPSKNKIISQSKPVCSTPIPSAVNKLTINKIKPNETLSKNHTGVRITQKYHDLLDNFFGYLEQFPISKLVEFFDDKSSLVSEILTERNINKKAMLALSDRSIVLETSSFQSITPGNSYGDIFDKDETVVDFDEQDEICENQFEPSVKSFDEWEDGDDVFESIDESLFIADTENFVGQLNAYRNENAPMINVTNAADLDGTSYVPINEKQSKWAIEDFEYKSHPNDGETAEFSGDFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLNGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGVLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTVETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVMEISNVGWGSTLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADFVVAHVVPGPKHQLLLSGNATVQMPISKPRTTKDKDKVIGKEPVKEVDRLQKECYQKLVEVARKFANEAKIQNYANLFSNQTLMEMSERLPADLNDLKDITSFPQVKINKYGCNFLEVTKHYNLLLTKHKQEMKDLEKKLTVDIKPENAKSSYFSSTSKAPAKGYNRKRKSPNNVFSKKFSNFKRSKTASSKSESVSKKKSAGLMKLTVRS 1034          
BLAST of ATP-dependent DNA helicase vs. Planmine SMEST
Match: SMESG000064966.1 (SMESG000064966.1)

HSP 1 Score: 274.248 bits (700), Expect = 1.989e-79
Identity = 176/570 (30.88%), Postives = 289/570 (50.70%), Query Frame = 2
Query: 1205 DFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQ---FMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIR-PKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCICDNCQNSNDNKVVMRDLTDHAKAVVKYVMEISNVGWGSTLNYVLDVFRGANNAKIVEKQGANNWLYGTGSVFSKNDAERF------------FHHLVFEGYLWEKMVVTQADFV-VAHVVPGPKHQLLLSGNATVQMPISKPRTTKDKDKV 2863
            +FP +  +    ++ F +  F+  Q + IN+TL   D   I   G GKSLCYQL ++I+ G T+VI+PL SLI DQ+   ++  I    L  +      ++I   +++     +  KL+Y TP+K+  S+KL    +     K L R VIDE HC S+  HDFR  Y+ L  L++ +PN+P++ LTATA   V  DI   L + ++      ++NR N+ +E++     KK   EI  L+ ++F  QSGIV+C ++ +C+ + + +  +G+   +Y+  +    +K +   W+NEN CK++VAT AFGMGIDKP+VRFV+H S+ KS+E +YQE+GRAGRDG  + C+LF+   +  ++   I+++ SG           L +M  Y  +   C+R  + +   E F    C      +C  C ++ ++++   D+ D  K+ +  +  I                    N KI   +  +  L    S++  + A RF            F HLV  GYL  K+  +   F    +V+ G K    L  N  + +PI   ++ K ++ V
Sbjct:   52 NFPWSSNIKKLLQDKFKIKNFKSLQLKTINATLDKLDCIFISSPGSGKSLCYQLASMIS-GFTLVIAPLGSLIQDQMIVSEKFGISCISLDSNSSVKIQNEIFKAMETDPVDNVTYKLIYTTPDKISNSKKLTKAIEKAYGDKRLVRLVIDEIHCCSELSHDFRQDYKNLHGLKSLYPNLPVLGLTATANNSVINDIKTTLNLNSDCLVHKDNYNRSNIFYEVKFLSNEKKEGEEICKLIKSRFDGQSGIVFCFNQKDCENLTNELVNNGILASSYYGTLGYDIKKDIHNKWLNEN-CKVIVATNAFGMGIDKPNVRFVIHMSMSKSLENYYQESGRAGRDGKRSDCILFWKLAEYFKIGSNIFTEKSGI--------PNLNIMNRYILDDCTCKRVSLSSILDEKFTVNDCKN----MCSVCADT-ESEMSSIDIADVYKSALTIIKNI----------------EKTKNPKITGNKMISE-LANQMSLYCADLASRFPGIDYKLFLDKAFCHLVLAGYL--KLDYSFGSFSNFTYVIAGSK---TLKSNEKIDIPIRISKSDKMRNLV 584          
BLAST of ATP-dependent DNA helicase vs. Planmine SMEST
Match: SMESG000028029.1 (SMESG000028029.1)

HSP 1 Score: 276.559 bits (706), Expect = 5.742e-78
Identity = 174/443 (39.28%), Postives = 255/443 (57.56%), Query Frame = 2
Query: 1223 RMMIAFKEVFGLNGFRRN-QKEAINSTL-SGFDTFVIMPTGGGKSLCYQLPAVITE-GVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQS-----QFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTF-PNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIR----PKTAKKCISEII-------NLVNAKFRD-QSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGTIETKKQHEQQ-------LFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCICDNCQN 2467
            ++    K  F  + F+   QKEAI + + S  D F+ MPTG GKSLCYQ PAV  E GV+I+ISPL +LIHDQ+  L + +I A  ++   K  E ++++  L +        +I+LLYVTPE+  ++    N+ +SL+++ LL+ FVIDEAHC+S+WGHDFRP Y  LG LR+T   N+P IALTATA  KV EDI+  L  KN+  + +  F R N+ ++++       +K+ I + I          N  + D  S IVYC  R  C+E+A ++   GL+ + YHAG+  + R+++Q  WIN     IV ATI+FGMG+DK +VR V H++IPKS+  +YQE+GRAGRDG  +   ++Y  ++           N+ T    KQ E++       L +M  Y E +  CR  ++  HF + F      A K  +CD C N
Sbjct:    5 KLYNVLKHTFQFDEFKSPLQKEAIENIIHSKNDVFISMPTGSGKSLCYQFPAVYFETGVSIIISPLLALIHDQILHLSKRNICARTINSKSKVDERNEVISDLTTFNCFKDATKIRLLYVTPEQC-QTSTFQNIAKSLSKKNLLNYFVIDEAHCLSEWGHDFRPDYLKLGQLRSTLLNNIPCIALTATANKKVSEDIIKCLNFKNHKTFRMPVF-RDNIFYDVKFTDLLTDSKRHIFDFIFKCLGCPTSNNCNWNDIGSCIVYCRKREHCEEMALLLTSYGLQAKPYHAGIKQSIREEIQLDWIN-GKFPIVAATISFGMGVDKSNVRVVCHWTIPKSLTAYYQESGRAGRDGKLSYSRIYYDPSET----------NTITFLINKQKEKERVKANAWLQIMRDYIE-IPKCRHLRIAEHFNDEF-----TACK-TLCDFCTN 427          
BLAST of ATP-dependent DNA helicase vs. Planmine SMEST
Match: SMESG000064967.1 (SMESG000064967.1)

HSP 1 Score: 247.669 bits (631), Expect = 3.064e-73
Identity = 135/343 (39.36%), Postives = 202/343 (58.89%), Query Frame = 2
Query: 1130 QSKWAIEDFEYKSHPNDGETAEFSGDFPHTERMMIAFKEVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQF---MQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIR-PKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSGLKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRF 2146
            + K A+E     +  ND    E +  FP +  +    +EVF ++ FR  Q + IN+TLS  D  +I PTGGGKSLCYQLP+++T G+TI +SPL SL+ DQ+   Q   I +  L  +      +++   +QS     ++ KL++VTPEK+ +S+KLM   +       +SR VIDE HC SQWGHDFRP Y+ L + ++ FP +P++ LTATAT  + +DI   L +  +   +   +NR NL + ++      K   EI  L+ +++  QSGIVYC ++ +C+ + + +  +G+    YHA  S   RK V   W+NE  C ++VATIAFGMGIDKP+VR 
Sbjct:    2 KKKQALEQHIKSAFVNDNTDYE-NDSFPWSLTVNKILQEVFKMDKFRALQLKTINTTLSKRDCILIKPTGGGKSLCYQLPSLLT-GLTIAVSPLVSLMQDQLLISQRFGIPSVSLDANCPVKLQNEVYKSMQSDPVDKLKYKLIFVTPEKISKSKKLMKAIEKSYADNRISRIVIDEIHCCSQWGHDFRPDYKGLHIFKSLFPKIPILGLTATATDSIIDDIKEILGLNADCLVYRDVYNRPNLFYNVKFLSNDDKDDKEICKLIKSRYLGQSGIVYCFTQKDCETLTNELVLNGINACFYHANTSYEYRKDVHSKWLNEE-CNVIVATIAFGMGIDKPNVRI 341          
BLAST of ATP-dependent DNA helicase vs. Planmine SMEST
Match: SMESG000054509.1 (SMESG000054509.1)

HSP 1 Score: 192.971 bits (489), Expect = 1.194e-49
Identity = 126/362 (34.81%), Postives = 194/362 (53.59%), Query Frame = 2
Query: 1244 EVFGLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVI----TEGVTIVISPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLLYVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHY-RLLGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNLRWEIRPKTAKKCISEIIN--------LVNAKFRDQSGI-VYCLSRNEC-----------DEVASVMAQSGLK--CQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVVHYSIPKSIEGFYQETGRAGRDGLPATCVLFYS 2248
            +++G + FR +Q++ I    +   T  I+PTG GKSLCYQ+PA I     + V +++SPL SL+ DQ+ KL + +I    L+  +K  E  K L ++    ++   + ++PE +VES  L     + ++   +S   IDE HC+S W H FRP Y R+  LLR  +     + L+AT TT     +   LK+         S     + +   P      ++E+IN        L +A F   + I +YC SRNE            + V  V+ +  LK   +AYHAG+  T+R ++Q M++ +   +++VATIAFGMG+DK D+R V+HYS P+S E + QE GRAGRD LPA  ++  S
Sbjct:  399 KIYGFSTFRNHQEKIIRRICASQSTVAILPTGSGKSLCYQIPAYIFQQTQQTVALIVSPLISLMQDQLAKLPK-EIQGISLNSTMKFEEKIKALKEINE--LKYAFILLSPEALVESNWL-----TADKLPPISFACIDEVHCLSDWSHHFRPSYLRVCKLLRDCYRVDCFLGLSATCTTSTIACVTKALKIDR------VSDADSCIVYNPIPDNITISVTEVINKELALIKLLQSAPFNTSASILIYCSSRNETMSLADLIQLKLESVEVVINKKILKWTARAYHAGLKSTERAKLQRMFM-KGRIRVLVATIAFGMGLDKSDIRAVIHYSRPRSFENYVQEIGRAGRDFLPANALVLLS 745          
The following BLAST results are available for this feature:
BLAST of ATP-dependent DNA helicase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
BLM0.000e+045.38BLM RecQ like helicase [Source:HGNC Symbol;Acc:HGN... [more]
BLM0.000e+045.38BLM RecQ like helicase [Source:HGNC Symbol;Acc:HGN... [more]
BLM4.052e-16051.80BLM RecQ like helicase [Source:HGNC Symbol;Acc:HGN... [more]
RECQL2.598e-12036.72RecQ like helicase [Source:HGNC Symbol;Acc:HGNC:99... [more]
RECQL2.598e-12036.72RecQ like helicase [Source:HGNC Symbol;Acc:HGNC:99... [more]
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BLAST of ATP-dependent DNA helicase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
him-68.464e-15341.37Bloom syndrome protein homolog [Source:UniProtKB/... [more]
K02F3.121.950e-11336.25Putative ATP-dependent DNA helicase Q1 [Source:Un... [more]
K02F3.121.950e-11336.25Putative ATP-dependent DNA helicase Q1 [Source:Un... [more]
K02F3.125.487e-11336.25Putative ATP-dependent DNA helicase Q1 [Source:Un... [more]
rcq-51.374e-8339.05ATP-dependent DNA helicase [Source:UniProtKB/TrEM... [more]
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BLAST of ATP-dependent DNA helicase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Blm0.000e+042.52gene:FBgn0002906 transcript:FBtr0082434[more]
RecQ59.801e-8136.43gene:FBgn0027375 transcript:FBtr0100362[more]
RecQ51.078e-8036.43gene:FBgn0027375 transcript:FBtr0075714[more]
RecQ59.334e-7736.43gene:FBgn0027375 transcript:FBtr0075715[more]
RecQ45.768e-5936.24gene:FBgn0040290 transcript:FBtr0076729[more]
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BLAST of ATP-dependent DNA helicase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
blm0.000e+045.24BLM RecQ like helicase [Source:ZFIN;Acc:ZDB-GENE-0... [more]
blm0.000e+045.24BLM RecQ like helicase [Source:ZFIN;Acc:ZDB-GENE-0... [more]
blm0.000e+045.24BLM RecQ like helicase [Source:ZFIN;Acc:ZDB-GENE-0... [more]
recql3.318e-11836.85RecQ helicase-like [Source:ZFIN;Acc:ZDB-GENE-05080... [more]
recql3.318e-11836.85RecQ helicase-like [Source:ZFIN;Acc:ZDB-GENE-05080... [more]
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BLAST of ATP-dependent DNA helicase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
blm0.000e+043.14Bloom syndrome RecQ like helicase [Source:NCBI gen... [more]
blm0.000e+044.84Bloom syndrome RecQ like helicase [Source:NCBI gen... [more]
blm0.000e+043.14Bloom syndrome RecQ like helicase [Source:NCBI gen... [more]
blm2.441e-16340.03Bloom syndrome RecQ like helicase [Source:NCBI gen... [more]
blm3.071e-16151.59Bloom syndrome RecQ like helicase [Source:NCBI gen... [more]
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BLAST of ATP-dependent DNA helicase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Blm0.000e+045.52Bloom syndrome, RecQ like helicase [Source:MGI Sym... [more]
Blm0.000e+045.52Bloom syndrome, RecQ like helicase [Source:MGI Sym... [more]
Recql3.063e-11836.11RecQ protein-like [Source:MGI Symbol;Acc:MGI:10302... [more]
Recql3.407e-11836.11RecQ protein-like [Source:MGI Symbol;Acc:MGI:10302... [more]
Recql3.838e-11836.11RecQ protein-like [Source:MGI Symbol;Acc:MGI:10302... [more]
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BLAST of ATP-dependent DNA helicase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P54132|BLM_HUMAN0.000e+045.38Bloom syndrome protein OS=Homo sapiens OX=9606 GN=... [more]
sp|Q9VGI8|BLM_DROME0.000e+042.52Bloom syndrome protein homolog OS=Drosophila melan... [more]
sp|Q9DEY9|BLM_XENLA0.000e+043.48Bloom syndrome protein homolog OS=Xenopus laevis O... [more]
sp|O88700|BLM_MOUSE0.000e+045.52Bloom syndrome protein homolog OS=Mus musculus OX=... [more]
sp|Q9I920|BLM_CHICK0.000e+044.68Bloom syndrome protein homolog OS=Gallus gallus OX... [more]
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BLAST of ATP-dependent DNA helicase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A074Z7F70.000e+047.25Uncharacterized protein OS=Opisthorchis viverrini ... [more]
A0A4S2M6C70.000e+046.58Uncharacterized protein OS=Opisthorchis felineus O... [more]
A0A3R7F8K60.000e+046.69Bloom syndrome-like protein (Fragment) OS=Clonorch... [more]
H2KNK10.000e+046.26ATP-dependent DNA helicase OS=Clonorchis sinensis ... [more]
A0A4Z2D6W00.000e+045.30Bloom syndrome protein OS=Schistosoma japonicum OX... [more]
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BLAST of ATP-dependent DNA helicase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
blm0.000e+044.44BLM RecQ like helicase [Source:NCBI gene;Acc:10304... [more]
recql2.255e-12235.80RecQ like helicase [Source:NCBI gene;Acc:103027659... [more]
recql5.908e-12135.65RecQ like helicase [Source:NCBI gene;Acc:103027659... [more]
recql8.186e-12135.53RecQ like helicase [Source:NCBI gene;Acc:103027659... [more]
recql53.610e-8940.28RecQ like helicase 5 [Source:NCBI gene;Acc:1030316... [more]
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BLAST of ATP-dependent DNA helicase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
blm0.000e+045.81BLM RecQ like helicase [Source:ZFIN;Acc:ZDB-GENE-0... [more]
recql3.965e-11937.69RecQ helicase-like [Source:ZFIN;Acc:ZDB-GENE-05080... [more]
recql51.301e-9040.55RecQ helicase-like 5 [Source:ZFIN;Acc:ZDB-GENE-071... [more]
ENSPMAT00000005409.17.173e-2444.03pep scaffold:Pmarinus_7.0:GL485130:385:11513:1 gen... [more]
ENSPMAT00000003898.11.063e-925.95pep scaffold:Pmarinus_7.0:GL481305:13824:21767:-1 ... [more]
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BLAST of ATP-dependent DNA helicase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5
Match NameE-valueIdentityDescription
SGS15.171e-13942.65RecQ family nucleolar DNA helicase; role in genome... [more]
HRQ11.519e-924.573'-5' DNA helicase that has DNA strand annealing a... [more]
DHH13.591e-921.07Cytoplasmic DEAD-box helicase, stimulates mRNA dec... [more]
FAL16.344e-923.01Nucleolar protein required for maturation of 18S r... [more]
DBP31.319e-823.41RNA-Dependent ATPase, member of DExD/H-box family;... [more]
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BLAST of ATP-dependent DNA helicase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO326321.072e-12241.84Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO340911.507e-10042.59Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO255202.658e-5838.33Predicted protein [Source:UniProtKB/TrEMBL;Acc:A8... [more]
EDO325371.933e-5133.79Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO464055.056e-4229.62Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of ATP-dependent DNA helicase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
blm0.000e+044.04BLM RecQ like helicase [Source:NCBI gene;Acc:10004... [more]
blm0.000e+044.04BLM RecQ like helicase [Source:NCBI gene;Acc:10004... [more]
recql1.620e-11135.58RecQ like helicase [Source:NCBI gene;Acc:101164117... [more]
recql1.620e-11135.58RecQ like helicase [Source:NCBI gene;Acc:101164117... [more]
recql1.620e-11135.58RecQ like helicase [Source:NCBI gene;Acc:101164117... [more]
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BLAST of ATP-dependent DNA helicase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000060148.10.000e+099.32SMESG000060148.1[more]
SMESG000064966.11.989e-7930.88SMESG000064966.1[more]
SMESG000028029.15.742e-7839.28SMESG000028029.1[more]
SMESG000064967.13.064e-7339.36SMESG000064967.1[more]
SMESG000054509.11.194e-4934.81SMESG000054509.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30000541 ID=SMED30000541|Name=ATP-dependent DNA helicase|organism=Schmidtea mediterranea sexual|type=transcript|length=3540bp
TTTACATATGGGATATAGATTTTGAAATTAGAAATTTTCTATAAGATATT
TCATGGCCCCTAAATTTAAATTTAAGTCTTTTAAATCTGACAACTTACAA
GGTGAAAAGCAAAATGTATCTCTTTTATCGGAATCTGTTTCATTTGATAA
ATCAAATAACAGTACAATATCCAATTCAATCAGTAATAACCAGTCTTCAA
ATTTAAATAAGGTATTGTTTTCACCTGAAATAAATCTAACTGCTAAATCA
CATCATTTCGAAATTACGCATAATAAAAATATGGAAATAAATAAAATTGA
AATAGCTAAAACTTTACCCACTGCCAAAATAATTCCACTACAAAATGTCT
CTCCCGCCAAACAAATTTTGCCTGAATCATCTACTTTATCTCTTGACGAT
CAAGATGTATTTAGTGATGATTTTTTAGTTGATAATCCGCCGAAACTATT
AAAACCTATTACTAAACCTAAAACAACACTTTCAACGTCTAAGACAAATT
TATCAAAACCTAGTAAAAATAAAATCATATCACAATCAAAGCCTGTTTGT
AGCACTCCTATACCTAGTGCAGTTAATAAATTGACAATCAATAAAATAAA
GCCAAACGAAACTCTATCTAAAAATCTCACCGGAGTTCGAATTACTCAGA
AATATCATGATTTGTTGGATAATTTTTTCGGTTACTTAGAGCAATTTCCC
ATCAGCAAGTTAGTCGAATTTTTCGATGACAAGAGTAGTTTAGTTTCAGA
AATATTAACTGAAAGAAATATCAATAAGAAGGCAATGTTAGCCTTGTCTG
ATAGGTCAATCGTTTTGGAAAGTTCGTCTTTTCAATCGATCACTCCTGGC
AATTCTTATGGAGATATTTTTGATAAAGATGAAACAGTTGTTGATTTTGA
CGAGCAAGATGAAATCTGTGAAAATCAATTTGAACCAAGTGTTAAATCAT
TTGATGAATGGGAAGACGGCGATGACGTTTTTGAAAGTATTGACGAATCA
TTATTCATTGCGGACACGGAAAACTTTGTAGGTCAATTGAATGCATATAG
AAACGAGAACGCTCCAATGATTAACGTCACAAACGCTGCAGATCTCGATG
GAACTAGCTACGTTCCTATTAATGAGAAGCAATCTAAATGGGCCATTGAA
GATTTCGAATACAAAAGCCATCCGAATGATGGGGAAACCGCAGAATTTTC
CGGGGATTTTCCTCACACAGAAAGGATGATGATAGCATTCAAGGAAGTGT
TTGGATTGAATGGATTCCGTCGGAACCAGAAGGAAGCAATAAATTCCACC
CTGAGTGGATTTGATACGTTCGTTATCATGCCAACCGGTGGAGGGAAAAG
TCTATGTTATCAGTTGCCGGCAGTGATTACCGAAGGAGTTACAATTGTCA
TTTCCCCATTGAAGTCATTAATCCACGATCAAGTTACGAAATTGCAAGAA
TTAGATATAGTCGCAACTCACTTATCGGGTGACTTGAAAGAGGCGGAAGT
CGACAAAATCCTGCTACAATTGCAATCACAATTCATGCAAATCAAATTGC
TCTATGTCACCCCGGAGAAGGTGGTCGAGTCGAGAAAACTGATGAATGTG
TTCCAGAGTTTAAACCAACGGAAATTGTTATCCCGGTTTGTTATTGACGA
GGCTCATTGTGTGAGCCAATGGGGCCACGATTTTCGGCCGCATTACCGAT
TATTGGGCTTGTTGCGGACGACATTCCCCAATGTGCCGATGATAGCTCTG
ACTGCAACGGCAACAACAAAAGTCCGTGAAGATATTCTCTATCAATTAAA
AATGAAAAACAACACGAAATGGTTCATCCAGAGTTTTAATCGAGGAAATC
TCCGATGGGAAATACGACCGAAAACCGCCAAGAAATGTATATCGGAAATA
ATTAATTTGGTCAATGCTAAATTCCGGGATCAGAGCGGGATTGTGTACTG
CTTATCGAGGAATGAGTGCGATGAAGTGGCGTCGGTGATGGCGCAATCCG
GTTTGAAGTGCCAGGCGTACCATGCGGGTATGTCGGACACAAAAAGGAAA
CAGGTTCAGGAAATGTGGATTAATGAGAACGGCTGCAAAATCGTGGTGGC
AACGATCGCATTCGGTATGGGCATAGACAAGCCAGATGTCAGATTTGTAG
TGCACTATTCCATCCCAAAATCTATTGAAGGCTTCTACCAGGAGACGGGA
AGGGCAGGTCGAGATGGGTTACCTGCAACCTGTGTGCTATTTTACTCCTG
GACGGACGTCGTGCGAATGCGCAAGCTCATATACAGCGATAATTCTGGAA
CCATCGAGACAAAGAAACAGCACGAGCAGCAACTTTTCCTAATGGTTTCA
TATTGCGAGAATCTAATTGATTGTCGGAGGACGCAAGTGCTCAACCACTT
TGGAGAGCACTTTGATCGAAAGCTCTGTGGCGCCGTCAAAGGATGTATCT
GTGACAACTGTCAGAACAGCAATGACAATAAAGTGGTGATGAGGGATCTG
ACAGATCACGCCAAAGCTGTCGTCAAATATGTAATGGAGATCAGCAATGT
TGGATGGGGATCAACTTTGAATTATGTGCTGGATGTATTCAGAGGAGCTA
ACAACGCGAAAATAGTTGAGAAGCAAGGTGCCAACAATTGGCTATATGGA
ACCGGTAGCGTGTTTAGCAAAAATGATGCAGAGCGATTTTTCCACCATCT
GGTATTTGAGGGTTATCTGTGGGAGAAAATGGTTGTAACTCAAGCTGATT
TCGTTGTGGCTCACGTGGTACCGGGCCCGAAACACCAATTACTGTTATCT
GGTAACGCTACGGTCCAGATGCCGATTTCCAAACCGCGCACCACCAAGGA
CAAGGATAAAGTTATCGGAAAGGAACCGGTTAAGGAAGTCGATCGATTAC
AGAAGGAATGCTATCAGAAATTGGTTGAGGTCGCAAGGAAATTCGCCAAT
GAAGCGAAAATTCAGAACTATGCAAATTTGTTTTCCAATCAAACGCTGAT
GGAAATGTCTGAGCGATTGCCCGCTGATCTCAATGATTTGAAGGACATCA
CCAGCTTCCCCCAGGTCAAGATCAATAAATACGGCTGCAATTTCCTAGAA
GTTACCAAACATTACAATCTTCTTTTAACCAAACACAAACAAGAAATGAA
AGATCTGGAGAAGAAGCTGACTGTTGATATTAAACCAGAAAATGCCAAAT
CCAGTTATTTTTCATCGACTTCCAAGGCTCCGGCTAAAGGTTATAATAGA
AAAAGGAAATCACCCAATAATGTATTTTCGAAAAAATTTTCAAATTTCAA
GCGCTCGAAAACAGCCAGTTCGAAGTCAGAGAGCGTATCAAAGAAAAAAT
CGGCAGGATTGATGAAATTGACCGTCCGCAGCTAATATAATTCTGAATGC
CTGTACAGGTGTATATAGTTGATGATGATGCTGCATCGTTTGTATTTTGA
TAGAAATATATGGTAACCCCGTGTTTTATTGCTTCGCATTTGATTTTTAT
TTTTTATTGGTGATTTTTATTCGTGATGTGTTATTGATTC
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protein sequence of SMED30000541-orf-1

>SMED30000541-orf-1 ID=SMED30000541-orf-1|Name=SMED30000541-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1111bp
MAPKFKFKSFKSDNLQGEKQNVSLLSESVSFDKSNNSTISNSISNNQSSN
LNKVLFSPEINLTAKSHHFEITHNKNMEINKIEIAKTLPTAKIIPLQNVS
PAKQILPESSTLSLDDQDVFSDDFLVDNPPKLLKPITKPKTTLSTSKTNL
SKPSKNKIISQSKPVCSTPIPSAVNKLTINKIKPNETLSKNLTGVRITQK
YHDLLDNFFGYLEQFPISKLVEFFDDKSSLVSEILTERNINKKAMLALSD
RSIVLESSSFQSITPGNSYGDIFDKDETVVDFDEQDEICENQFEPSVKSF
DEWEDGDDVFESIDESLFIADTENFVGQLNAYRNENAPMINVTNAADLDG
TSYVPINEKQSKWAIEDFEYKSHPNDGETAEFSGDFPHTERMMIAFKEVF
GLNGFRRNQKEAINSTLSGFDTFVIMPTGGGKSLCYQLPAVITEGVTIVI
SPLKSLIHDQVTKLQELDIVATHLSGDLKEAEVDKILLQLQSQFMQIKLL
YVTPEKVVESRKLMNVFQSLNQRKLLSRFVIDEAHCVSQWGHDFRPHYRL
LGLLRTTFPNVPMIALTATATTKVREDILYQLKMKNNTKWFIQSFNRGNL
RWEIRPKTAKKCISEIINLVNAKFRDQSGIVYCLSRNECDEVASVMAQSG
LKCQAYHAGMSDTKRKQVQEMWINENGCKIVVATIAFGMGIDKPDVRFVV
HYSIPKSIEGFYQETGRAGRDGLPATCVLFYSWTDVVRMRKLIYSDNSGT
IETKKQHEQQLFLMVSYCENLIDCRRTQVLNHFGEHFDRKLCGAVKGCIC
DNCQNSNDNKVVMRDLTDHAKAVVKYVMEISNVGWGSTLNYVLDVFRGAN
NAKIVEKQGANNWLYGTGSVFSKNDAERFFHHLVFEGYLWEKMVVTQADF
VVAHVVPGPKHQLLLSGNATVQMPISKPRTTKDKDKVIGKEPVKEVDRLQ
KECYQKLVEVARKFANEAKIQNYANLFSNQTLMEMSERLPADLNDLKDIT
SFPQVKINKYGCNFLEVTKHYNLLLTKHKQEMKDLEKKLTVDIKPENAKS
SYFSSTSKAPAKGYNRKRKSPNNVFSKKFSNFKRSKTASSKSESVSKKKS
AGLMKLTVRS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0003678DNA helicase activity
GO:0004386helicase activity
GO:0016787hydrolase activity
GO:00431383'-5' DNA helicase activity
GO:0043140ATP-dependent 3'-5' DNA helicase activity
GO:0003676nucleic acid binding
GO:0005524ATP binding
GO:0008026ATP-dependent helicase activity
GO:0003824catalytic activity
GO:0000166nucleotide binding
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
GO:0005622intracellular
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR036388WH-like_DNA-bd_sf
IPR004589DNA_helicase_ATP-dep_RecQ
IPR036390WH_DNA-bd_sf
IPR018982RQC_domain
IPR014001Helicase_ATP-bd
IPR001650Helicase_C
IPR002121HRDC_dom
IPR027417P-loop_NTPase
IPR011991WHTH_DNA-bd_dom
IPR032284RecQ_Zn-bd
IPR011545DEAD/DEAH_box_helicase_dom
IPR002464DNA/RNA_helicase_DEAH_CS
IPR010997HRDC-like_sf
Vocabulary: biological process
TermDefinition
GO:0006260DNA replication
GO:0006281DNA repair
GO:0044237cellular metabolic process
GO:0006310DNA recombination
GO:0032508DNA duplex unwinding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 1018..1038
NoneNo IPR availableGENE3DG3DSA:3.40.50.300coord: 380..596
e-value: 2.4E-87
score: 293.6
NoneNo IPR availableGENE3DG3DSA:1.10.150.80coord: 942..1023
e-value: 8.7E-19
score: 69.2
NoneNo IPR availableGENE3DG3DSA:3.40.50.300coord: 597..809
e-value: 2.2E-66
score: 225.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1074..1097
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1052..1110
NoneNo IPR availablePANTHERPTHR13710DNA HELICASE RECQ FAMILY MEMBERcoord: 350..1096
NoneNo IPR availablePANTHERPTHR13710:SF128ATP-DEPENDENT DNA HELICASE Q-LIKE 4Acoord: 350..1096
NoneNo IPR availableCDDcd18016DEXHc_RecQ2_BLMcoord: 388..596
e-value: 1.82792E-106
score: 329.865
NoneNo IPR availableCDDcd18794SF2_C_RecQcoord: 597..731
e-value: 1.52433E-64
score: 212.069
IPR014001Helicase superfamily 1/2, ATP-binding domainSMARTSM00487ultradead3coord: 401..605
e-value: 1.3E-23
score: 94.4
IPR014001Helicase superfamily 1/2, ATP-binding domainPROSITEPS51192HELICASE_ATP_BIND_1coord: 413..588
score: 22.493
IPR001650Helicase, C-terminalSMARTSM00490helicmild6coord: 640..722
e-value: 1.0E-22
score: 91.4
IPR001650Helicase, C-terminalPFAMPF00271Helicase_Ccoord: 614..721
e-value: 4.0E-15
score: 56.1
IPR001650Helicase, C-terminalPROSITEPS51194HELICASE_CTERcoord: 615..762
score: 19.796
IPR018982RQC domainSMARTSM00956RQC_2coord: 815..927
e-value: 3.0E-10
score: 50.1
IPR018982RQC domainPFAMPF09382RQCcoord: 813..928
e-value: 3.2E-16
score: 59.1
IPR002121HRDC domainSMARTSM00341hrdc7coord: 947..1028
e-value: 1.9E-4
score: 30.8
IPR002121HRDC domainPFAMPF00570HRDCcoord: 950..1017
e-value: 2.6E-11
score: 43.3
IPR002121HRDC domainPROSITEPS50967HRDCcoord: 947..1028
score: 14.877
IPR011545DEAD/DEAH box helicase domainPFAMPF00270DEADcoord: 407..574
e-value: 6.5E-19
score: 68.4
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3DG3DSA:1.10.10.10coord: 812..926
e-value: 6.0E-27
score: 95.6
IPR004589DNA helicase, ATP-dependent, RecQ typeTIGRFAMTIGR00614TIGR00614coord: 396..868
e-value: 7.5E-174
score: 576.9
IPR032284ATP-dependent DNA helicase RecQ, zinc-binding domainPFAMPF16124RecQ_Zn_bindcoord: 734..804
e-value: 3.4E-15
score: 56.5
IPR002464DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved sitePROSITEPS00690DEAH_ATP_HELICASEcoord: 527..536
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILYSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 445..740
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILYSSF46785"Winged helix" DNA-binding domaincoord: 813..918
IPR010997HRDC-like superfamilySUPERFAMILYSSF47819HRDC-likecoord: 946..1021