SMED30000514

Overview
NameSMED30000514
Smed IDSMED30000514
Length (bp)3832
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30000514 (SMED30000514) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30000514 (SMED30000514) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30000514 (SMED30000514) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30000514 (SMED30000514) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30000514

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
protonephridiaSMED30000514h1SMcG0014040 dd_Smed_v4_10854_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30000514h1SMcG0014040 dd_Smed_v4_10854_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30000514h1SMcG0014040 dd_Smed_v4_10854_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30000514 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000794:72767..107147 -18581
Homology
BLAST of SMED30000514 vs. TrEMBL
Match: A0A267E659 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig017121g2 PE=4 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 6.452e-15
Identity = 83/390 (21.28%), Postives = 171/390 (43.85%), Query Frame = 1
Query: 1870 VSGMRLFLN--RENVSQFVLAVRGFDVIRAVKIIQNQPEF----RNKAMCTRFKENSYKEMCKYFG----PRLNEEQIKELV-EKDNSRYVANELSYGISLYNSLCWSQP-TDVMLLVSPMILDIGWILNILYEKGGYLLINIVRINLSSST-GRQIGIPENELDNFEKKNGSCHLLVIRNENPLH-SLKFVLRECMIFMNNNELN--ENRFFYMTPYSDALMNILDIRRSFQTPEVRELISNMSDAPFDDCERCAFVLIISDEFTKHAAQILDQMTNLDLLAIKCFANELDQDHMNLIKSQFNIRFPNLSDDSEADGPEIRMRIGVIAIRSMYVIRELEKIHNTIKIEKNLIFAYTITVEASFRMIASFFTSMELVSDPRRRSTLKFLP 2991
            ++G+R+  +  +   +   +AVRG   + A++ +          R+    +R+ E+  +E+ +YFG    P++ + Q++ L  ++++   VA  L+           + P  DV +L SP++ +IGW++++  E GG+ LI   R+ L        +G+ +++   F   NG   +L++R EN LH +  F+        +  EL    +  F+  P   A++  +   R    P+++       +  + D E+ A +++    F   AA++LDQ+   +LL +KC      Q H + +  Q          +  A  P + +      IR + ++ +L+ I        +    ++ +   ++ +  + F   EL     RR  L   P
Sbjct:  700 LAGVRMIYSAAKSQAATLAIAVRGCGAVAAMRSVAASGGVFEGRRDGWAVSRYAESVPRELARYFGGRLSPKMTQSQVRALAPDREDLSCVARLLT-----------AAPLEDVAMLASPLVTEIGWVIHLASE-GGFALIGCRRVQLHRDELANLLGMADSQAACF--SNGVACVLLLRRENALHHAGAFIQNVVKEIKSRRELGVPASLLFFAMPCLSAVLQTVHCTRIDSLPKLKSDAHRPEEPRYADQEQLAVLIVSGRNFQAIAAELLDQLAEFELLGLKCLP-RFPQLHAHFVAEQLLGGS-GGGSEWRAAKPLMELPALTSVIRGVGLMHQLDAIAPHYPACSDYRLLHSTSWHQAYCLTRNLFADFELFECSERRPCLLTCP 1073          
BLAST of SMED30000514 vs. TrEMBL
Match: A0A267FCV8 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig017121g1 PE=4 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 1.002e-14
Identity = 83/390 (21.28%), Postives = 171/390 (43.85%), Query Frame = 1
Query: 1870 VSGMRLFLN--RENVSQFVLAVRGFDVIRAVKIIQNQPEF----RNKAMCTRFKENSYKEMCKYFG----PRLNEEQIKELV-EKDNSRYVANELSYGISLYNSLCWSQP-TDVMLLVSPMILDIGWILNILYEKGGYLLINIVRINLSSST-GRQIGIPENELDNFEKKNGSCHLLVIRNENPLH-SLKFVLRECMIFMNNNELN--ENRFFYMTPYSDALMNILDIRRSFQTPEVRELISNMSDAPFDDCERCAFVLIISDEFTKHAAQILDQMTNLDLLAIKCFANELDQDHMNLIKSQFNIRFPNLSDDSEADGPEIRMRIGVIAIRSMYVIRELEKIHNTIKIEKNLIFAYTITVEASFRMIASFFTSMELVSDPRRRSTLKFLP 2991
            ++G+R+  +  +   +   +AVRG   + A++ +          R+    +R+ E+  +E+ +YFG    P++ + Q++ L  ++++   VA  L+           + P  DV +L SP++ +IGW++++  E G + LI   R+ L        +G+ +++   F   NG   +L++R EN LH +  F+        +  EL    +  F+  P   A++  +   R    P+++       +  + D E+ A +++    F   AA++LDQ+   +LL +KC      Q H + +  Q          +  A  P + +      IR + ++ +L+ I        +    ++I+   ++ +  + F   EL     RR  L   P
Sbjct:  700 LAGVRMIYSAAKSQAATLAIAVRGCGAVTAMRSVAASGGVFEGRRDGWAVSRYAESVPRELARYFGGRLSPKMTQSQVRALAPDREDLSCVARLLT-----------AAPLEDVAMLASPLVTEIGWVIHLASESG-FALIGCRRVQLHRDELANLLGMADSQAACF--SNGVACVLLLRRENALHHAGAFIQNVVKEIKSRRELGVPASLLFFAMPCLSAVLQTVHCTRIDSLPKLKSDAHRPDEPRYADQEQLAVLIVSGRNFQAIAAELLDQLAEFELLGLKCLP-RFPQLHAHFVAEQLLGGS-GGGSEWRAAKPLMELPALTFVIRGVGLMHQLDAIAPHYPACSDYRLLHSISWHQAYCLTRNLFADFELFECSERRPCLLTCP 1073          
BLAST of SMED30000514 vs. TrEMBL
Match: A0A267FZV4 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig017121g3 PE=4 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 2.631e-14
Identity = 82/390 (21.03%), Postives = 170/390 (43.59%), Query Frame = 1
Query: 1870 VSGMRLFLN--RENVSQFVLAVRGFDVIRAVKIIQNQPEF----RNKAMCTRFKENSYKEMCKYFG----PRLNEEQIKELV-EKDNSRYVANELSYGISLYNSLCWSQP-TDVMLLVSPMILDIGWILNILYEKGGYLLINIVRINLSSST-GRQIGIPENELDNFEKKNGSCHLLVIRNENPLH-SLKFVLRECMIFMNNNELN--ENRFFYMTPYSDALMNILDIRRSFQTPEVRELISNMSDAPFDDCERCAFVLIISDEFTKHAAQILDQMTNLDLLAIKCFANELDQDHMNLIKSQFNIRFPNLSDDSEADGPEIRMRIGVIAIRSMYVIRELEKIHNTIKIEKNLIFAYTITVEASFRMIASFFTSMELVSDPRRRSTLKFLP 2991
            ++G+R+  +  +   +   +AVRG   + A++ +          R+    +R+ E+  +E+ +YFG    P++ + Q++ L  ++++   VA  L+           + P  DV +L SP++ +IGW++++  E G + LI   R+ L        +G+ +++   F   NG   +L++R EN LH +  F+        +  EL    +  F+  P   A++  +   R    P+++       +  + D E+ A +++    F   AA++LDQ+   +LL +KC      Q H + +  Q          +  A  P + +      IR + ++ +L+ I        +    ++ +   ++ +  + F   EL     RR  L   P
Sbjct:  700 LAGVRMIYSAAKSQAATLAIAVRGCGAVTAMRSVAASGGVFEGRRDGWAVSRYAESVPRELARYFGGRLSPKMTQSQVRALAPDREDLSCVARLLT-----------AAPLEDVAMLASPLVTEIGWVIHLASESG-FALIGCRRVQLHRDELANLLGMADSQAACF--SNGVACVLLLRRENALHHAGAFIQNVVKEIKSRRELGVPASLLFFAMPCLSAVLQTVHCTRIDSLPKLKSDAHRPDEPRYPDQEQLAVLIVSGRNFQAIAAELLDQLAEFELLGLKCLP-RFPQLHAHFVAEQLLGGS-GGGSEWRAAKPLMELPALTFVIRGVGLMHQLDAIAPHYPACSDYRLLHSTSWHQAYCLTRNLFADFELFECSERRPCLLTCP 1073          
BLAST of SMED30000514 vs. TrEMBL
Match: A0A1I8GL26 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 1.422e-9
Identity = 62/278 (22.30%), Postives = 120/278 (43.17%), Query Frame = 1
Query: 2170 DVMLLVSPMILDIGWILNILYEKGGYLLINIVRINLS-SSTGRQIGIPENELDNFEKKNGSCHLLVIRNENPLH-SLKFVLRECMIFMNNNELN--ENRFFYMTPYSDALMNILDIRRSFQTPEVRELISNMSDAPFDDCERCAFVLIISDEFTKHAAQILDQMTNLDLLAIKCFANELDQDHMNLIKSQFNIRFPNLSDDSEADGPEIRMRIGVIAIRSMYVIRELEKIHNTIKIEKNLIFAYTITVEASFRMIASFFTSMELVSDPRRRSTLKFLP 2991
            DV +L SP++ +IGW++++  E GG+ LI   R+ L        +G+ +++   F   NG   +L++R EN LH +  F+        +  EL    +  F+  P   A++  +   R    P+++       +  + D E+ A +++    F   AA++LDQ+   +LL +KC      Q H + +  Q          +  A  P + +      IR + ++ +L+ I        +    ++ +   ++ +  + F   EL     RR  L   P
Sbjct:   30 DVAMLASPLVTEIGWVIHLASE-GGFALIGCRRVQLHRDELANLLGMADSQAACF--SNGVACVLLLRRENALHHAGAFIQNVVKEIKSRRELGVPASLLFFAMPCLSAVLQTVHCTRIDSLPKLKSDAHRPEEPRYADQEQLAVLIVSGRNFQAIAAELLDQLAEFELLGLKCLP-RFPQLHAHFVAEQLLGGS-GGGSEWRAAKPLMELPALTSVIRGVGLMHQLDAIAPHYPACSDYRLLHSTSWHQAYCLTRNLFADFELFECSERRPCLLTCP 302          
BLAST of SMED30000514 vs. TrEMBL
Match: A0A1I8H432 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 1.536e-9
Identity = 62/278 (22.30%), Postives = 120/278 (43.17%), Query Frame = 1
Query: 2170 DVMLLVSPMILDIGWILNILYEKGGYLLINIVRINLS-SSTGRQIGIPENELDNFEKKNGSCHLLVIRNENPLH-SLKFVLRECMIFMNNNELN--ENRFFYMTPYSDALMNILDIRRSFQTPEVRELISNMSDAPFDDCERCAFVLIISDEFTKHAAQILDQMTNLDLLAIKCFANELDQDHMNLIKSQFNIRFPNLSDDSEADGPEIRMRIGVIAIRSMYVIRELEKIHNTIKIEKNLIFAYTITVEASFRMIASFFTSMELVSDPRRRSTLKFLP 2991
            DV +L SP++ +IGW++++  E G + LI   R+ L        +G+ +++   F   NG   +L++R EN LH +  F+        +  EL    +  F+  P   A++  +   R    P+++       +  + D E+ A +++    F   AA++LDQ+   +LL +KC      Q H + +  Q          +  A  P + +      IR + ++ +L+ I        +    ++I+   ++ +  + F   EL     RR  L   P
Sbjct:   30 DVAMLASPLVTEIGWVIHLASESG-FALIGCRRVQLHRDELANLLGMADSQAACF--SNGVACVLLLRRENALHHAGAFIQNVVKEIKSRRELGVPASLLFFAMPCLSAVLQTVHCTRIDSLPKLKSDAHRPDEPRYADQEQLAVLIVSGRNFQAIAAELLDQLAEFELLGLKCLP-RFPQLHAHFVAEQLLGGS-GGGSEWRAAKPLMELPALTFVIRGVGLMHQLDAIAPHYPACSDYRLLHSISWHQAYCLTRNLFADFELFECSERRPCLLTCP 302          
BLAST of SMED30000514 vs. Planmine SMEST
Match: SMESG000036214.1 (SMESG000036214.1)

HSP 1 Score: 1963.35 bits (5085), Expect = 0.000e+0
Identity = 1063/1068 (99.53%), Postives = 1065/1068 (99.72%), Query Frame = 1
Query:  274 KRQKDLSEKIINTNMNCELPFNNIKLPTGILHGQPVNTQDQIETNERVILSNFGVRDDIDLDKILEEMDLSNYQTTFFEEILMDXXXXXXXXXXXXDNSKSQLLKYLDKMSQQYEMMSKPNNSNNNCSQKPPKSKSNLSLECLFDQMKQEKGIKTVYLDLRSENHLFCQINQNLNPSADNTIKPVDDCKXXXXXXXXXXXXXPNISLWIDNYRKSKSADKKNNLKNSNDAKNKEIKSENKLIYNEIQVENVRQEIVFDDAKERNHVDLENDDKVPEHXXXXXXXXXXXXXXXSERRFIINQLKELVEHSDSYPVLLHEEVTFESNTNKLTPELIADSENKYFNIFISISPPNNVMTISKVQSPFSVDHPKENFIALISLILKSTDQNSKTHLPFQVVAFXXXXXXXXXXXXXXXXXXXXXXXXRMDLRNCTLRNIFNDNNENCEDSNQVLSNLPLSNIFRLQPHDVXXXXXXXXXXXXXXXXHKISVKGAICESNSKTRNLDTNTSSVILGKEFFMSEMFKTFLHLLLNRFTVSGMRLFLNRENVSQFVLAVRGFDVIRAVKIIQNQPEFRNKAMCTRFKENSYKEMCKYFGPRLNEEQIKELVEKDNSRYVANELSYGISLYNSLCWSQPTDVMLLVSPMILDIGWILNILYEKGGYLLINIVRINLSSSTGRQIGIPENELDNFEKKNGSCHLLVIRNENPLHSLKFVLRECMIFMNNNELNENRFFYMTPYSDALMNILDIRRSFQTPEVRELISNMSDAPFDDCERCAFVLIISDEFTKHAAQILDQMTNLDLLAIKCFANELDQDHMNLIKSQFNIRFPNLSDDSEADGPEIRMRIGVIAIRSMYVIRELEKIHNTIKIEKNLIFAYTITVEASFRMIASFFTSMELVSDPRRRSTLKFLPESLVKDLAQKSIIAQFPILYSSIKTIFIVQINKSESVLSRFRKFWFLLSRDGFSIVKSSLDSNGKLYFIVERPNAIFYLIQWMKYHFSDEFDSKIEICANPEDFELFLRKIPTNITYHSVLSMNRTTNCELALLVIKCNLLQSSVEMLSNTKMFWNMLEDNK 3477
            K ++DLSEKIINTNMNCELPFNNIKLPTGILHGQPVNTQDQIETNERVILSNFGVRDDIDLDKILEEMDLSNYQTTFFEEILMDPEEKIPETEITTDNSKSQLLKYLDKMSQQYEMMSKPNNSNNNCSQKPPKSKSNLSLECLFDQMKQEKGIKTVYLDLRSENHLFCQINQNLNPSADNTIKPVDDCKDSSDDDEEESNENPNISLWIDNYRKSKSADKKNNLKNSNDAKNKEIKSENKLIYNEIQVENVRQEIVFDDAKERNHVDLENDDKVPEHIQLKIEKIKQQEKILSERRFIINQLKELVEHSDSYPVLLHEEVTFESNTNKLTPELIADSENKYFNIFISISPPNNVMTISKVQSPFSVDHPKENFIALISLILKSTDQNSKTHLPFQVVAFQQIYYQNQLQLALLCLSDLSLTKLRMDLRNCTLRNIFNDNNENCEDSNQVLSNLPLSNIFRLQPHDVLLTNNIWLWNNSSIIWHKISVKGAICESNSKTRNLDTNTSSVILGKEFFMSEMFKTFLHLLLNRFTVSGMRLFLN EN SQFVLAVRGFDVIRAVKIIQNQPEFRNKAMCTRFKENSYKEMCKYFGPRLNEEQIKELVEKDNSRYVANELSYGISLYNSLCWSQPTDVMLLVSPMILDIGWILNILYEKGGYLLINIVRINLSSSTGRQIGIPENELDNFEKKNGSCHLLVIRNENPLHSLKFVLRECMIFMNNNELNENRFFYMTPYSDALMNILDIRRSFQTPEVRELISNMSDAPFDDCERCAFVLIISDEFTKHAAQILDQMTNLDLLAIKCFANELDQDHMNLIKSQFNIRFPNLSDDSEADGPEIRMRIGVIAIRSMYVIRELEKIHNTIKIEKNLIFAYTITVEASFRMIASFFTSMELVSDPRRRSTLKFLPESLVKDLAQKSIIAQFPILYSSIKTIFIVQINKSESVLSRFRKFWFLLSRDGFSIVKSSLDSNGKLYFIVERPNAIFYLIQWMKYHFSDEFDSKIEICANPEDFELFLRKIPTNITYHSVLSMNRTTNCELALLVIKCNLLQSSVEMLSNTKMFWNMLEDNK
Sbjct:   75 KDKEDLSEKIINTNMNCELPFNNIKLPTGILHGQPVNTQDQIETNERVILSNFGVRDDIDLDKILEEMDLSNYQTTFFEEILMDPEEKIPETEITTDNSKSQLLKYLDKMSQQYEMMSKPNNSNNNCSQKPPKSKSNLSLECLFDQMKQEKGIKTVYLDLRSENHLFCQINQNLNPSADNTIKPVDDCKDSSDDDEEESNENPNISLWIDNYRKSKSADKKNNLKNSNDAKNKEIKSENKLIYNEIQVENVRQEIVFDDAKERNHVDLENDDKVPEHIQLKIEKIKQQEKILSERRFIINQLKELVEHSDSYPVLLHEEVTFESNTNKLTPELIADSENKYFNIFISISPPNNVMTISKVQSPFSVDHPKENFIALISLILKSTDQNSKTHLPFQVVAFQQIYYQNQLQLALLCLSDLSLTKLRMDLRNCTLRNIFNDNNENCEDSNQVLSNLPLSNIFRLQPHDVLLTNNIWLWNNSSIIWHKISVKGAICESNSKTRNLDTNTSSVILGKEFFMSEMFKTFLHLLLNRFTVSGMRLFLNGENASQFVLAVRGFDVIRAVKIIQNQPEFRNKAMCTRFKENSYKEMCKYFGPRLNEEQIKELVEKDNSRYVANELSYGISLYNSLCWSQPTDVMLLVSPMILDIGWILNILYEKGGYLLINIVRINLSSSTGRQIGIPENELDNFEKKNGSCHLLVIRNENPLHSLKFVLRECMIFMNNNELNENRFFYMTPYSDALMNILDIRRSFQTPEVRELISNMSDAPFDDCERCAFVLIISDEFTKHAAQILDQMTNLDLLAIKCFANELDQDHMNLIKSQFNIRFPNLSDDSEADGPEIRMRIGVIAIRSMYVIRELEKIHNTIKIEKNLIFAYTITVEASFRMIASFFTSMELVSDPRRRSTLKFLPESLVKDLAQKSIIAQFPILYSSIKTIFIVQINKSESVLSRFRKFWFLLSRDGFSIVKSSLDSNGKLYFIVERPNAIFYLIQWMKYHFSDEFDSKIEICANPEDFELFLRKIPTNITYHSVLSMNRTTNCELALLVIKCNLLQSSVEMLSNTKMFWNMLEDNK 1142          

HSP 2 Score: 213.772 bits (543), Expect = 5.534e-56
Identity = 106/106 (100.00%), Postives = 106/106 (100.00%), Query Frame = 3
Query: 3513 IQYLALSVVDVNGSMIYKLRNIAILNPSGVQLAKETRYLCIVAAGRISSIITSDLIKSNLGFMKSDSELSDDVYANYDFEQIIQCCEILLLHESDSIESISNRNVI 3830
            IQYLALSVVDVNGSMIYKLRNIAILNPSGVQLAKETRYLCIVAAGRISSIITSDLIKSNLGFMKSDSELSDDVYANYDFEQIIQCCEILLLHESDSIESISNRNVI
Sbjct: 1143 IQYLALSVVDVNGSMIYKLRNIAILNPSGVQLAKETRYLCIVAAGRISSIITSDLIKSNLGFMKSDSELSDDVYANYDFEQIIQCCEILLLHESDSIESISNRNVI 1248          

HSP 3 Score: 153.68 bits (387), Expect = 2.952e-37
Identity = 76/76 (100.00%), Postives = 76/76 (100.00%), Query Frame = 2
Query:   53 MEGSIYNNISKEINFQLPEINFDSDSDEIDDNQPIKPFIRPSAEILFGNDDSDNEEIFNLLSKLPHQESDVANEKD 280
            MEGSIYNNISKEINFQLPEINFDSDSDEIDDNQPIKPFIRPSAEILFGNDDSDNEEIFNLLSKLPHQESDVANEKD
Sbjct:    1 MEGSIYNNISKEINFQLPEINFDSDSDEIDDNQPIKPFIRPSAEILFGNDDSDNEEIFNLLSKLPHQESDVANEKD 76          
The following BLAST results are available for this feature:
BLAST of SMED30000514 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000514 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000514 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000514 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000514 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000514 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000514 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000514 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267E6596.452e-1521.28Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267FCV81.002e-1421.28Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267FZV42.631e-1421.03Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8GL261.422e-922.30Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8H4321.536e-922.30Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of SMED30000514 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000514 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000514 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000514 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000514 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000514 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000036214.15.534e-5699.53SMESG000036214.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30000514 ID=SMED30000514|Name=SMED30000514|organism=Schmidtea mediterranea sexual|type=transcript|length=3832bp
TTCCGATCTATTAGATACCAAATTTGCGTTAAATATTTAAATTAATTTGA
AAATGGAAGGAAGTATTTACAATAATATTTCAAAGGAAATAAATTTTCAA
TTGCCAGAAATAAACTTTGATTCAGATTCTGATGAAATTGATGATAATCA
GCCAATTAAGCCTTTTATTCGACCATCTGCTGAGATATTATTTGGAAACG
ATGATTCAGATAACGAGGAAATCTTTAACTTATTATCAAAATTACCTCAC
CAAGAATCAGATGTTGCAAATGAAAAAGACAAAAAGATTTGTCTGAAAAG
ATTATTAATACGAATATGAATTGTGAATTGCCATTTAATAATATTAAGTT
ACCAACTGGAATATTACATGGACAACCTGTTAATACGCAAGATCAAATAG
AGACTAATGAAAGAGTAATTTTGTCTAATTTCGGGGTTCGGGATGACATT
GACCTTGACAAAATACTAGAAGAAATGGATTTATCCAATTATCAAACGAC
ATTTTTCGAAGAAATTCTAATGGATCCCGAAGAAAAGATTCCAGAGACTG
AGATAACCACTGATAATTCCAAAAGCCAGTTATTGAAATATCTAGATAAA
ATGTCCCAACAATATGAAATGATGTCCAAACCCAATAATTCCAACAATAA
TTGCTCACAGAAACCCCCTAAATCAAAATCCAATCTGTCATTGGAATGTC
TTTTTGATCAAATGAAACAAGAAAAGGGAATCAAAACAGTTTATTTGGAT
TTGCGTTCAGAAAACCATTTATTCTGTCAAATAAACCAAAATCTTAACCC
CAGTGCTGATAATACCATCAAACCCGTTGATGATTGTAAGGATTCAAGTG
ATGATGATGAGGAGGAATCGAATGAAAATCCCAATATCAGCTTGTGGATT
GATAATTACCGCAAATCAAAAAGTGCTGACAAAAAGAATAATTTAAAAAA
CTCAAATGATGCAAAAAATAAAGAAATTAAATCTGAAAATAAACTGATTT
ATAATGAAATCCAAGTGGAAAATGTTCGTCAAGAAATTGTGTTTGATGAT
GCAAAAGAACGTAACCATGTGGACCTTGAAAACGATGACAAAGTTCCTGA
ACATATTCAATTAAAAATTGAAAAAATTAAGCAACAGGAAAAGATTTTAT
CTGAAAGAAGATTCATTATTAATCAATTGAAAGAATTAGTTGAACATTCT
GATTCTTATCCTGTCTTGCTTCATGAGGAAGTGACTTTCGAATCCAATAC
AAATAAATTAACTCCTGAATTAATTGCCGACTCGGAAAATAAATATTTCA
ATATTTTCATATCTATTTCTCCACCTAATAACGTGATGACAATCTCCAAA
GTGCAATCTCCTTTTAGTGTCGATCACCCAAAAGAAAATTTCATTGCTCT
TATTTCCTTGATATTGAAGTCCACAGATCAAAATTCTAAAACTCATTTAC
CATTCCAAGTTGTTGCTTTTCAGCAGATTTATTATCAAAACCAATTACAA
TTGGCACTTCTATGTTTGTCTGATCTATCTTTGACAAAATTACGAATGGA
TCTAAGAAACTGTACTTTAAGAAATATTTTCAATGATAATAATGAAAATT
GTGAAGATTCCAATCAAGTTTTGAGTAACCTTCCTTTATCAAACATATTT
CGACTACAACCGCATGATGTTTTGTTGACAAACAATATCTGGCTTTGGAA
CAACAGTTCTATTATTTGGCATAAAATCTCAGTAAAAGGAGCAATTTGTG
AATCGAATTCGAAGACAAGGAATCTTGATACTAATACATCATCAGTTATT
TTAGGAAAAGAATTTTTCATGTCAGAAATGTTTAAAACATTTCTTCATTT
ATTGTTAAACAGATTTACTGTATCTGGAATGAGGCTGTTCTTAAATAGAG
AGAATGTTAGCCAGTTCGTTTTAGCTGTCAGAGGATTTGACGTAATACGA
GCGGTCAAGATTATACAAAATCAACCAGAGTTTAGAAACAAAGCTATGTG
TACTAGATTCAAAGAAAACTCTTATAAAGAAATGTGTAAATATTTTGGAC
CGAGATTAAATGAAGAACAAATTAAAGAATTAGTTGAAAAAGATAATTCT
CGATATGTTGCTAATGAATTGAGTTATGGAATCAGTTTGTATAATTCTTT
ATGTTGGTCACAACCAACGGATGTTATGCTATTGGTATCACCAATGATTC
TGGACATAGGATGGATATTAAATATACTGTACGAGAAAGGCGGCTATTTA
TTAATAAATATTGTTCGAATCAATTTATCTTCATCAACTGGAAGGCAAAT
CGGTATACCCGAGAATGAACTTGATAATTTCGAAAAGAAAAATGGTAGTT
GTCATTTATTGGTGATTCGTAATGAAAATCCATTACATTCTTTAAAATTT
GTGCTTCGAGAATGTATGATTTTCATGAATAATAATGAATTGAATGAAAA
CAGATTTTTCTACATGACTCCATATTCTGATGCATTAATGAATATACTGG
ATATTCGACGCTCTTTTCAAACTCCTGAGGTTCGAGAGTTAATTTCAAAT
ATGTCCGATGCTCCATTTGACGACTGCGAACGATGCGCTTTTGTATTGAT
AATCAGTGACGAGTTTACGAAACATGCTGCTCAAATTCTTGATCAAATGA
CAAATTTAGACCTCTTAGCCATTAAATGCTTTGCCAATGAACTAGATCAA
GATCACATGAACTTAATAAAATCTCAATTTAACATCCGATTTCCCAATTT
ATCAGACGATTCCGAAGCCGACGGACCAGAAATTAGGATGAGAATCGGAG
TCATTGCGATCCGCAGCATGTATGTGATTCGTGAATTGGAGAAAATTCAC
AACACCATCAAAATTGAAAAGAATTTAATATTTGCATATACCATTACAGT
CGAAGCAAGTTTCAGAATGATTGCATCATTTTTCACCAGTATGGAGTTGG
TTAGTGATCCAAGACGCAGGTCAACCCTGAAATTCTTACCGGAATCACTA
GTCAAAGATCTCGCTCAAAAATCAATTATTGCTCAATTTCCAATATTATA
TTCATCGATTAAAACAATTTTCATTGTACAAATTAATAAATCGGAATCTG
TATTGTCAAGATTCAGAAAATTCTGGTTCTTGCTGTCGAGAGATGGCTTC
TCTATTGTCAAATCTTCACTAGACAGCAATGGAAAACTCTATTTCATTGT
CGAACGGCCAAATGCGATATTTTATCTAATTCAGTGGATGAAGTATCATT
TTAGTGATGAATTTGACAGCAAAATTGAAATTTGTGCCAATCCTGAAGAT
TTTGAGTTATTTTTAAGAAAAATTCCAACAAATATTACTTACCATTCTGT
ATTGTCAATGAATCGGACTACAAATTGTGAGTTAGCACTCCTCGTGATAA
AGTGTAATCTGCTCCAATCTTCTGTAGAAATGCTTTCTAATACAAAGATG
TTCTGGAATATGTTAGAAGATAATAAACGGAGAGGAAAGCATAGAGAACA
GTGTGTCAATGAATTCAATATCTTGCTTTATCTGTGGTTGATGTTAACGG
ATCTATGATTTACAAGTTACGTAACATTGCTATTCTTAATCCATCAGGAG
TTCAATTAGCAAAGGAAACAAGATATTTGTGCATTGTAGCCGCAGGGAGA
ATTTCTTCTATTATCACGAGCGACTTGATAAAATCAAATTTAGGCTTTAT
GAAATCGGATTCAGAGTTAAGTGATGATGTCTATGCGAATTATGATTTCG
AACAGATCATTCAGTGCTGTGAAATTTTGCTGTTACATGAATCGGATTCA
ATCGAGTCTATTTCGAATAGAAATGTTATCAA
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protein sequence of SMED30000514-orf-1

>SMED30000514-orf-1 ID=SMED30000514-orf-1|Name=SMED30000514-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1081bp
MNCELPFNNIKLPTGILHGQPVNTQDQIETNERVILSNFGVRDDIDLDKI
LEEMDLSNYQTTFFEEILMDPEEKIPETEITTDNSKSQLLKYLDKMSQQY
EMMSKPNNSNNNCSQKPPKSKSNLSLECLFDQMKQEKGIKTVYLDLRSEN
HLFCQINQNLNPSADNTIKPVDDCKDSSDDDEEESNENPNISLWIDNYRK
SKSADKKNNLKNSNDAKNKEIKSENKLIYNEIQVENVRQEIVFDDAKERN
HVDLENDDKVPEHIQLKIEKIKQQEKILSERRFIINQLKELVEHSDSYPV
LLHEEVTFESNTNKLTPELIADSENKYFNIFISISPPNNVMTISKVQSPF
SVDHPKENFIALISLILKSTDQNSKTHLPFQVVAFQQIYYQNQLQLALLC
LSDLSLTKLRMDLRNCTLRNIFNDNNENCEDSNQVLSNLPLSNIFRLQPH
DVLLTNNIWLWNNSSIIWHKISVKGAICESNSKTRNLDTNTSSVILGKEF
FMSEMFKTFLHLLLNRFTVSGMRLFLNRENVSQFVLAVRGFDVIRAVKII
QNQPEFRNKAMCTRFKENSYKEMCKYFGPRLNEEQIKELVEKDNSRYVAN
ELSYGISLYNSLCWSQPTDVMLLVSPMILDIGWILNILYEKGGYLLINIV
RINLSSSTGRQIGIPENELDNFEKKNGSCHLLVIRNENPLHSLKFVLREC
MIFMNNNELNENRFFYMTPYSDALMNILDIRRSFQTPEVRELISNMSDAP
FDDCERCAFVLIISDEFTKHAAQILDQMTNLDLLAIKCFANELDQDHMNL
IKSQFNIRFPNLSDDSEADGPEIRMRIGVIAIRSMYVIRELEKIHNTIKI
EKNLIFAYTITVEASFRMIASFFTSMELVSDPRRRSTLKFLPESLVKDLA
QKSIIAQFPILYSSIKTIFIVQINKSESVLSRFRKFWFLLSRDGFSIVKS
SLDSNGKLYFIVERPNAIFYLIQWMKYHFSDEFDSKIEICANPEDFELFL
RKIPTNITYHSVLSMNRTTNCELALLVIKCNLLQSSVEMLSNTKMFWNML
EDNKRRGKHREQCVNEFNILLYLWLMLTDL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000099neuron
PLANA:0002032epidermal cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 69..83
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 69..89