Ubiquitin thioesterase

Overview
NameUbiquitin thioesterase
Smed IDSMED30000494
Length (bp)960
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Ubiquitin thioesterase (SMED30000494) t-SNE clustered cells

Violin plots show distribution of expression levels for Ubiquitin thioesterase (SMED30000494) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Ubiquitin thioesterase (SMED30000494) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Ubiquitin thioesterase (SMED30000494) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30000494

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
posterior sideSMED30000494SMESG000043359.1 dd_Smed_v4_5846_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30000494SMESG000043359.1 dd_Smed_v4_5846_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Ubiquitin thioesterase vs. Ensembl Human
Match: OTUB1 (OTU deubiquitinase, ubiquitin aldehyde binding 1 [Source:HGNC Symbol;Acc:HGNC:23077])

HSP 1 Score: 181.03 bits (458), Expect = 4.326e-55
Identity = 95/217 (43.78%), Postives = 140/217 (64.52%), Query Frame = 2 
Query:  275 NSYLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVENCQNSGDIQCLTFTPSHIDSQSKDEIFINSYITLLYRPGHYDILYE 925
            N Y +K+K L  KY  +R+ R DGNCF+R+FGFS LE+LL   ++    R K      K  L+ QG++EFTI+DF+  F+++++ ++  + ++ L   FN+Q  SDY +V+LRLL S YLQ  + +++ FIE G T+K+FC++EVEPM  ESD+IHIIAL+Q+L V+I VE        +  T  P      S+ +++      LLYRPGHYDILY+
Sbjct:   36 NIYQQKIKDLHKKYSYIRKTRPDGNCFYRAFGFSHLEALL--DDSKELQRFKAVSAKSKEDLVSQGFTEFTIEDFHNTFMDLIEQVEKQTSVADLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEGGRTVKEFCQQEVEPMCKESDHIHIIALAQALSVSIQVEYMDRG---EGGTTNPHIFPEGSEPKVY------LLYRPGHYDILYK 241
[Close]
BLAST of Ubiquitin thioesterase vs. Ensembl Human
Match: OTUB1 (OTU deubiquitinase, ubiquitin aldehyde binding 1 [Source:HGNC Symbol;Acc:HGNC:23077])

HSP 1 Score: 181.03 bits (458), Expect = 9.974e-55
Identity = 95/217 (43.78%), Postives = 140/217 (64.52%), Query Frame = 2 
Query:  275 NSYLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVENCQNSGDIQCLTFTPSHIDSQSKDEIFINSYITLLYRPGHYDILYE 925
            N Y +K+K L  KY  +R+ R DGNCF+R+FGFS LE+LL   ++    R K      K  L+ QG++EFTI+DF+  F+++++ ++  + ++ L   FN+Q  SDY +V+LRLL S YLQ  + +++ FIE G T+K+FC++EVEPM  ESD+IHIIAL+Q+L V+I VE        +  T  P      S+ +++      LLYRPGHYDILY+
Sbjct:   66 NIYQQKIKDLHKKYSYIRKTRPDGNCFYRAFGFSHLEALL--DDSKELQRFKAVSAKSKEDLVSQGFTEFTIEDFHNTFMDLIEQVEKQTSVADLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEGGRTVKEFCQQEVEPMCKESDHIHIIALAQALSVSIQVEYMDRG---EGGTTNPHIFPEGSEPKVY------LLYRPGHYDILYK 271
[Close]
BLAST of Ubiquitin thioesterase vs. Ensembl Human
Match: OTUB1 (OTU deubiquitinase, ubiquitin aldehyde binding 1 [Source:HGNC Symbol;Acc:HGNC:23077])

HSP 1 Score: 181.03 bits (458), Expect = 9.974e-55
Identity = 95/217 (43.78%), Postives = 140/217 (64.52%), Query Frame = 2 
Query:  275 NSYLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVENCQNSGDIQCLTFTPSHIDSQSKDEIFINSYITLLYRPGHYDILYE 925
            N Y +K+K L  KY  +R+ R DGNCF+R+FGFS LE+LL   ++    R K      K  L+ QG++EFTI+DF+  F+++++ ++  + ++ L   FN+Q  SDY +V+LRLL S YLQ  + +++ FIE G T+K+FC++EVEPM  ESD+IHIIAL+Q+L V+I VE        +  T  P      S+ +++      LLYRPGHYDILY+
Sbjct:   66 NIYQQKIKDLHKKYSYIRKTRPDGNCFYRAFGFSHLEALL--DDSKELQRFKAVSAKSKEDLVSQGFTEFTIEDFHNTFMDLIEQVEKQTSVADLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEGGRTVKEFCQQEVEPMCKESDHIHIIALAQALSVSIQVEYMDRG---EGGTTNPHIFPEGSEPKVY------LLYRPGHYDILYK 271
[Close]
BLAST of Ubiquitin thioesterase vs. Ensembl Human
Match: OTUB1 (OTU deubiquitinase, ubiquitin aldehyde binding 1 [Source:HGNC Symbol;Acc:HGNC:23077])

HSP 1 Score: 180.644 bits (457), Expect = 1.858e-54
Identity = 95/217 (43.78%), Postives = 140/217 (64.52%), Query Frame = 2 
Query:  275 NSYLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVENCQNSGDIQCLTFTPSHIDSQSKDEIFINSYITLLYRPGHYDILYE 925
            N Y +K+K L  KY  +R+ R DGNCF+R+FGFS LE+LL   ++    R K      K  L+ QG++EFTI+DF+  F+++++ ++  + ++ L   FN+Q  SDY +V+LRLL S YLQ  + +++ FIE G T+K+FC++EVEPM  ESD+IHIIAL+Q+L V+I VE        +  T  P      S+ +++      LLYRPGHYDILY+
Sbjct:   75 NIYQQKIKDLHKKYSYIRKTRPDGNCFYRAFGFSHLEALL--DDSKELQRFKAVSAKSKEDLVSQGFTEFTIEDFHNTFMDLIEQVEKQTSVADLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEGGRTVKEFCQQEVEPMCKESDHIHIIALAQALSVSIQVEYMDRG---EGGTTNPHIFPEGSEPKVY------LLYRPGHYDILYK 280
[Close]
BLAST of Ubiquitin thioesterase vs. Ensembl Human
Match: OTUB1 (OTU deubiquitinase, ubiquitin aldehyde binding 1 [Source:HGNC Symbol;Acc:HGNC:23077])

HSP 1 Score: 179.104 bits (453), Expect = 4.983e-54
Identity = 96/217 (44.24%), Postives = 139/217 (64.06%), Query Frame = 2 
Query:  275 NSYLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVENCQNSGDIQCLTFTPSHIDSQSKDEIFINSYITLLYRPGHYDILYE 925
            N Y +K+K L  KY  +R+ R DGNCF+R+FGFS LE+LL  S      R K      K  L+ QG++EFTI+DF+  F+++++ ++  + ++ L   FN+Q  SDY +V+LRLL S YLQ  + +++ FIE G T+K+FC++EVEPM  ESD+IHIIAL+Q+L V+I VE        +  T  P      S+ +++      LLYRPGHYDILY+
Sbjct:   66 NIYQQKIKDLHKKYSYIRKTRPDGNCFYRAFGFSHLEALLDDSKELQ-RRFKAVSAKSKEDLVSQGFTEFTIEDFHNTFMDLIEQVEKQTSVADLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEGGRTVKEFCQQEVEPMCKESDHIHIIALAQALSVSIQVEYMDRG---EGGTTNPHIFPEGSEPKVY------LLYRPGHYDILYK 272
[Close]
BLAST of Ubiquitin thioesterase vs. Ensembl Celegans
Match: otub-1 (Ubiquitin thioesterase otubain-like [Source:UniProtKB/Swiss-Prot;Acc:Q9XVR6])

HSP 1 Score: 131.724 bits (330), Expect = 4.154e-36
Identity = 73/224 (32.59%), Postives = 129/224 (57.59%), Query Frame = 2 
Query:  278 SYLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIF---NNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVENCQNSGDIQCLTFTPS---HIDSQSKDEIFINSYITLLYRPGHYDILYEND 931
            ++L K   L   Y  +R +RGDGNCF+R+     +E +LK  + +  ++       +   L++ G+ ++T  DF + F+E ++  K+ S +   E ++   N+ G ++Y ++F RL+ S++L+ N+  Y  FI+EGMT+ Q+CE+E+EPM+ ++D++ I +L ++    + +E           T  P+   H D  S D+  I   ITLLYRPGHYD++Y+ D
Sbjct:   64 AFLSKATELSEVYGEIRYIRGDGNCFYRAILVGLIEIMLK--DRARLEKFIASSRDWTRTLVELGFPDWTCTDFCDFFIEFLE--KIHSGVHTEEAVYTILNDDGSANYILMFFRLITSAFLKQNSEEYAPFIDEGMTVAQYCEQEIEPMWKDADHLAINSLIKAAGTRVRIEYMDR-------TAAPNGGWHYDIPSDDQQ-IAPEITLLYRPGHYDVIYKKD 275
[Close]
BLAST of Ubiquitin thioesterase vs. Ensembl Celegans
Match: otub-1 (Ubiquitin thioesterase otubain-like [Source:UniProtKB/Swiss-Prot;Acc:Q9XVR6])

HSP 1 Score: 131.724 bits (330), Expect = 4.154e-36
Identity = 73/224 (32.59%), Postives = 129/224 (57.59%), Query Frame = 2 
Query:  278 SYLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIF---NNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVENCQNSGDIQCLTFTPS---HIDSQSKDEIFINSYITLLYRPGHYDILYEND 931
            ++L K   L   Y  +R +RGDGNCF+R+     +E +LK  + +  ++       +   L++ G+ ++T  DF + F+E ++  K+ S +   E ++   N+ G ++Y ++F RL+ S++L+ N+  Y  FI+EGMT+ Q+CE+E+EPM+ ++D++ I +L ++    + +E           T  P+   H D  S D+  I   ITLLYRPGHYD++Y+ D
Sbjct:   64 AFLSKATELSEVYGEIRYIRGDGNCFYRAILVGLIEIMLK--DRARLEKFIASSRDWTRTLVELGFPDWTCTDFCDFFIEFLE--KIHSGVHTEEAVYTILNDDGSANYILMFFRLITSAFLKQNSEEYAPFIDEGMTVAQYCEQEIEPMWKDADHLAINSLIKAAGTRVRIEYMDR-------TAAPNGGWHYDIPSDDQQ-IAPEITLLYRPGHYDVIYKKD 275
[Close]
BLAST of Ubiquitin thioesterase vs. Ensembl Fly
Match: CG4968 (gene:FBgn0032214 transcript:FBtr0079987)

HSP 1 Score: 172.555 bits (436), Expect = 6.665e-52
Identity = 108/254 (42.52%), Postives = 154/254 (60.63%), Query Frame = 2 
Query:  191 IQNEIKETQXXXXXXXXXXXXXKDFGGHNSYLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDIS------KLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVE--NCQNSGDIQCLTFTPSHIDSQSKDEIFINSYITLLYRPGHYDILYEN 928
            I+ EI +T PL+SE LPL+ L  ++ G   +  K++ L  KY+ +RR R DGNCFFR+F +S+LE L+  SN SA+   K+  E  K  L+Q G+  FT++DF+E F+E++  +  D+         +L +IFN QG+SDY +V+LRL+ S  LQ  A +YQ FIE  +T++ F   EVEPMY ESD+IHIIAL  +L   + VE  +    G ++   F      S+ +        I L+YRPGHYDILY N
Sbjct:   22 IEKEISDTTPLVSEQLPLTCLYAEYSGDEIFTAKIQDLSKKYKFIRRTRPDGNCFFRAFAYSYLEYLI--SNTSAYQEFKKLAEESKEKLVQLGFPSFTLEDFHETFMEVIQRVSPDNAGGHSTVQDELHKIFNEQGYSDYVVVYLRLITSGKLQEEADFYQNFIEGDLTIEAFRHLEVEPMYKESDHIHIIALCTALGAGVRVEYLDRGEGGTVKAHDFPEG---SEPR--------IYLIYRPGHYDILYPN 262
[Close]
BLAST of Ubiquitin thioesterase vs. Ensembl Zebrafish
Match: otub1a (OTU deubiquitinase, ubiquitin aldehyde binding 1a [Source:ZFIN;Acc:ZDB-GENE-040718-108])

HSP 1 Score: 173.711 bits (439), Expect = 3.641e-52
Identity = 95/217 (43.78%), Postives = 134/217 (61.75%), Query Frame = 2 
Query:  281 YLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVE--NCQNSGDIQCLTFTPSHIDSQSKDEIFINSYITLLYRPGHYDILYE 925
            Y +K++ L  KY  +R+ R DGNCF+R+FGFS LESLL+ S      R K      K  L+ QG++EFTI+DF+  F+++++     + + +L   FN Q  SDY +V+LRL+ S YLQ    ++Q FIE G T+++FC++EVEPM  ESD+IHIIAL+Q+L V I VE  +    G +    F         + +IF      LLYRPGHYDILY+
Sbjct:   64 YQQKIRDLQKKYSSVRKTRPDGNCFYRAFGFSHLESLLEDSKE--LHRFKAIAAKSKLDLVSQGFTEFTIEDFHNTFMDLIEACDKQTSVGELLTSFNEQSVSDYLVVYLRLVTSGYLQREEDFFQHFIEGGRTVREFCQQEVEPMSKESDHIHIIALAQALGVCIQVEYMDRGEGGTVNHHIFPEG-----GEPKIF------LLYRPGHYDILYK 267
[Close]
BLAST of Ubiquitin thioesterase vs. Ensembl Zebrafish
Match: otub1b (OTU deubiquitinase, ubiquitin aldehyde binding 1b [Source:ZFIN;Acc:ZDB-GENE-040718-204])

HSP 1 Score: 171.014 bits (432), Expect = 3.554e-51
Identity = 105/249 (42.17%), Postives = 153/249 (61.45%), Query Frame = 2 
Query:  188 RIQNEIKETQXXXXXXXXXXXXXKDFGGHNS-YLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVE--NCQNSGDIQCLTFTPSHIDSQSKDEIFINSYITLLYRPGHYDILYE 925
            RIQ EI  + PL+S+   LS L+ ++   ++ Y  K++ L  KY  +R+ R DGNCF+R+FGF+ LESLL+ S      R K      K  L+ QG++EFTI+DF+  F+++++  +    + ++   FN+Q  SDY +V+LRLL S YLQ    ++Q FIE G ++K+FC++EVEPM  ESD+IHIIAL+Q+L V + VE  +    G +    F        S+  IF      LLYRPGHYDILY+
Sbjct:   31 RIQQEIATSNPLVSDRQELSVLKGEYAMEDTIYQLKIQDLHKKYSSIRKTRPDGNCFYRAFGFAHLESLLEDSKE--LQRFKAVAAKSKLDLVNQGFTEFTIEDFHNTFMDLIELCEKQQSLGEVLSSFNDQSVSDYIVVYLRLLTSGYLQREHVFFQHFIEGGRSVKEFCQQEVEPMSKESDHIHIIALAQALNVPVLVEYMDRGEGGTVNNHVFPEG-----SEPRIF------LLYRPGHYDILYK 266
[Close]
BLAST of Ubiquitin thioesterase vs. Ensembl Zebrafish
Match: otub1b (OTU deubiquitinase, ubiquitin aldehyde binding 1b [Source:ZFIN;Acc:ZDB-GENE-040718-204])

HSP 1 Score: 154.836 bits (390), Expect = 1.511e-45
Identity = 88/201 (43.78%), Postives = 132/201 (65.67%), Query Frame = 2 
Query:  188 RIQNEIKETQXXXXXXXXXXXXXKDFGGHNS-YLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVE 787
            RIQ EI  + PL+S+   LS L+ ++   ++ Y  K++ L  KY  +R+ R DGNCF+R+FGF+ LESLL+ S      R K      K  L+ QG++EFTI+DF+  F+++++  +    + ++   FN+Q  SDY +V+LRLL S YLQ    ++Q FIE G ++K+FC++EVEPM  ESD+IHIIAL+Q+L V + VE
Sbjct:    6 RIQQEIATSNPLVSDRQELSVLKGEYAMEDTIYQLKIQDLHKKYSSIRKTRPDGNCFYRAFGFAHLESLLEDSKE--LQRFKAVAAKSKLDLVNQGFTEFTIEDFHNTFMDLIELCEKQQSLGEVLSSFNDQSVSDYIVVYLRLLTSGYLQREHVFFQHFIEGGRSVKEFCQQEVEPMSKESDHIHIIALAQALNVPVLVE 204
[Close]
BLAST of Ubiquitin thioesterase vs. Ensembl Zebrafish
Match: otub1a (OTU deubiquitinase, ubiquitin aldehyde binding 1a [Source:ZFIN;Acc:ZDB-GENE-040718-108])

HSP 1 Score: 122.479 bits (306), Expect = 4.230e-33
Identity = 69/173 (39.88%), Postives = 109/173 (63.01%), Query Frame = 2 
Query:  188 RIQNEIKETQXXXXXXXXXXXXXKDFGGHNS-YLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEK 703
            +IQ EI  +  L+SE   LS L++++   ++ Y +K++ L  KY  +R+ R DGNCF+R+FGFS LESLL+ S      R K      K  L+ QG++EFTI+DF+  F+++++     + + +L   FN Q  SDY +V+LRL+ S YLQ    ++Q FIE G T+++FC++
Sbjct:   31 KIQQEIATSISLVSERQELSVLKREYADEDTIYQQKIRDLQKKYSSVRKTRPDGNCFYRAFGFSHLESLLEDSKE--LHRFKAIAAKSKLDLVSQGFTEFTIEDFHNTFMDLIEACDKQTSVGELLTSFNEQSVSDYLVVYLRLVTSGYLQREEDFFQHFIEGGRTVREFCQQ 201
[Close]
BLAST of Ubiquitin thioesterase vs. Ensembl Xenopus
Match: OTUB1 (OTU deubiquitinase, ubiquitin aldehyde binding 1 [Source:NCBI gene;Acc:100492748])

HSP 1 Score: 184.496 bits (467), Expect = 3.866e-56
Identity = 97/215 (45.12%), Postives = 139/215 (64.65%), Query Frame = 2 
Query:  281 YLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVENCQNSGDIQCLTFTPSHIDSQSKDEIFINSYITLLYRPGHYDILYE 925
            Y EK+K L  +Y  +RR R DGNCF+R+FGF++LE+LL         R KE     K+ LI+QG++EFTI+DF+  F+E++  ++    +++L   FN Q  SDY +V+LRLL S +LQ ++ +YQ FIE G ++K+FC++EVEPM  ESD+IHII+LSQ+L V+I VE          +T    H+  +  D       + LLYRPGHYDILY+
Sbjct:   64 YQEKIKDLHRRYNHIRRTRPDGNCFYRAFGFAYLEALL--GGGPELARFKEVSLQSKDELIRQGFTEFTIEDFHNTFMELIALVEKRPAVTELLAAFNEQTVSDYVVVYLRLLTSGHLQKDSIFYQHFIEGGRSVKEFCQQEVEPMCKESDHIHIISLSQALNVSIQVEYMDRGEGGTTVT----HVFPEGSD----PPQVFLLYRPGHYDILYK 268
[Close]
BLAST of Ubiquitin thioesterase vs. Ensembl Xenopus
Match: mcm3 (minichromosome maintenance complex component 3 [Source:Xenbase;Acc:XB-GENE-5857845])

HSP 1 Score: 124.405 bits (311), Expect = 2.138e-33
Identity = 75/207 (36.23%), Postives = 115/207 (55.56%), Query Frame = 2 
Query:  281 YLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLL-KQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVENCQNSGDIQCLTFTPSHIDSQSKDEIFINSYITLLYR 898
            Y  KLK L  +Y  +R+ R DG+CF+R   F++LE LL KQ     F  +  +    K+ ++  G+ E   +  YE FL +V+  ++D  IS L   FN Q  SD  + ++RL+ S+YL+  A +YQ FI EGMT+  FC + VE M +E D+I IIAL+Q+L + + VE    + ++      P    S S   +F+  +  +LYR
Sbjct:   25 YQRKLKDLQNQYHSVRKTRADGSCFYRGLCFAYLEFLLGKQQEILRFKHLVVQS---KSEVVMAGFQEHLFKHHYETFLSIVEMAELDGSISSLLTAFNQQCSSDSVVQYVRLVASAYLRNRAEFYQHFIREGMTVVDFCAQNVEQMAMECDHIQIIALTQALEIPLQVEYVDKTENVINHHIFPEGC-SPSVFMLFLEHHFNILYR 227
[Close]
BLAST of Ubiquitin thioesterase vs. Ensembl Xenopus
Match: mcm3 (minichromosome maintenance complex component 3 [Source:Xenbase;Acc:XB-GENE-5857845])

HSP 1 Score: 124.405 bits (311), Expect = 2.547e-33
Identity = 75/207 (36.23%), Postives = 115/207 (55.56%), Query Frame = 2 
Query:  281 YLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLL-KQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVENCQNSGDIQCLTFTPSHIDSQSKDEIFINSYITLLYR 898
            Y  KLK L  +Y  +R+ R DG+CF+R   F++LE LL KQ     F  +  +    K+ ++  G+ E   +  YE FL +V+  ++D  IS L   FN Q  SD  + ++RL+ S+YL+  A +YQ FI EGMT+  FC + VE M +E D+I IIAL+Q+L + + VE    + ++      P    S S   +F+  +  +LYR
Sbjct:   27 YQRKLKDLQNQYHSVRKTRADGSCFYRGLCFAYLEFLLGKQQEILRFKHLVVQS---KSEVVMAGFQEHLFKHHYETFLSIVEMAELDGSISSLLTAFNQQCSSDSVVQYVRLVASAYLRNRAEFYQHFIREGMTVVDFCAQNVEQMAMECDHIQIIALTQALEIPLQVEYVDKTENVINHHIFPEGC-SPSVFMLFLEHHFNILYR 229
[Close]
BLAST of Ubiquitin thioesterase vs. Ensembl Mouse
Match: Otub1 (OTU domain, ubiquitin aldehyde binding 1 [Source:MGI Symbol;Acc:MGI:2147616])

HSP 1 Score: 181.03 bits (458), Expect = 2.582e-55
Identity = 95/217 (43.78%), Postives = 140/217 (64.52%), Query Frame = 2 
Query:  275 NSYLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVENCQNSGDIQCLTFTPSHIDSQSKDEIFINSYITLLYRPGHYDILYE 925
            N Y +K+K L  KY  +R+ R DGNCF+R+FGFS LE+LL   ++    R K      K  L+ QG++EFTI+DF+  F+++++ ++  + ++ L   FN+Q  SDY +V+LRLL S YLQ  + +++ FIE G T+K+FC++EVEPM  ESD+IHIIAL+Q+L V+I VE        +  T  P      S+ +++      LLYRPGHYDILY+
Sbjct:   36 NIYQQKIKDLHKKYSYIRKTRPDGNCFYRAFGFSHLEALL--DDSKELQRFKAVSAKSKEDLVSQGFTEFTIEDFHNTFMDLIEQVEKQTSVADLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEGGRTVKEFCQQEVEPMCKESDHIHIIALAQALSVSIQVEYMDRG---EGGTTNPHVFPEGSEPKVY------LLYRPGHYDILYK 241
[Close]
BLAST of Ubiquitin thioesterase vs. Ensembl Mouse
Match: Otub1 (OTU domain, ubiquitin aldehyde binding 1 [Source:MGI Symbol;Acc:MGI:2147616])

HSP 1 Score: 181.415 bits (459), Expect = 5.014e-55
Identity = 95/217 (43.78%), Postives = 140/217 (64.52%), Query Frame = 2 
Query:  275 NSYLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVENCQNSGDIQCLTFTPSHIDSQSKDEIFINSYITLLYRPGHYDILYE 925
            N Y +K+K L  KY  +R+ R DGNCF+R+FGFS LE+LL   ++    R K      K  L+ QG++EFTI+DF+  F+++++ ++  + ++ L   FN+Q  SDY +V+LRLL S YLQ  + +++ FIE G T+K+FC++EVEPM  ESD+IHIIAL+Q+L V+I VE        +  T  P      S+ +++      LLYRPGHYDILY+
Sbjct:   66 NIYQQKIKDLHKKYSYIRKTRPDGNCFYRAFGFSHLEALL--DDSKELQRFKAVSAKSKEDLVSQGFTEFTIEDFHNTFMDLIEQVEKQTSVADLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEGGRTVKEFCQQEVEPMCKESDHIHIIALAQALSVSIQVEYMDRG---EGGTTNPHVFPEGSEPKVY------LLYRPGHYDILYK 271
[Close]
BLAST of Ubiquitin thioesterase vs. Ensembl Mouse
Match: Otub2 (OTU domain, ubiquitin aldehyde binding 2 [Source:MGI Symbol;Acc:MGI:1915399])

HSP 1 Score: 144.821 bits (364), Expect = 2.188e-41
Identity = 79/225 (35.11%), Postives = 129/225 (57.33%), Query Frame = 2 
Query:  257 KDFGGHNSYLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVENCQNSGDIQCLTFTPSHIDSQSKDEIFINSYIT---LLYRPGHYDILY 922
            +D   +  Y  K++ L  ++  +R+ +GDGNCF+R+ G+S+LESLL +S      + KER     N L+  G+ E   ++F+  F  +V+ ++ DS +S L ++FN+Q  SD  + FLRLL S++++  A +++ FI+E M +K FC  EVEPM +E D++ I ALSQ+L + + VE                 +D+     +F  + I    LLY+  HY+ILY
Sbjct:   20 RDHPENRIYQRKIQELSKRFTSIRKTKGDGNCFYRALGYSYLESLLGKSREIL--KFKERVLQTPNDLLAAGFEEHKFRNFFNAFYSVVELVEKDSSVSSLLKVFNDQSSSDRIVQFLRLLTSAFIRNRADFFRHFIDEEMDIKDFCTHEVEPMAMECDHVQITALSQALNIALQVEYVDE-------------MDTALNHHVFPEAAIPSVYLLYKTSHYNILY 229
[Close]
BLAST of Ubiquitin thioesterase vs. Ensembl Mouse
Match: Otub2 (OTU domain, ubiquitin aldehyde binding 2 [Source:MGI Symbol;Acc:MGI:1915399])

HSP 1 Score: 145.206 bits (365), Expect = 1.690e-40
Identity = 79/225 (35.11%), Postives = 129/225 (57.33%), Query Frame = 2 
Query:  257 KDFGGHNSYLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVENCQNSGDIQCLTFTPSHIDSQSKDEIFINSYIT---LLYRPGHYDILY 922
            +D   +  Y  K++ L  ++  +R+ +GDGNCF+R+ G+S+LESLL +S      + KER     N L+  G+ E   ++F+  F  +V+ ++ DS +S L ++FN+Q  SD  + FLRLL S++++  A +++ FI+E M +K FC  EVEPM +E D++ I ALSQ+L + + VE                 +D+     +F  + I    LLY+  HY+ILY
Sbjct:  109 RDHPENRIYQRKIQELSKRFTSIRKTKGDGNCFYRALGYSYLESLLGKSRE--ILKFKERVLQTPNDLLAAGFEEHKFRNFFNAFYSVVELVEKDSSVSSLLKVFNDQSSSDRIVQFLRLLTSAFIRNRADFFRHFIDEEMDIKDFCTHEVEPMAMECDHVQITALSQALNIALQVEYVDE-------------MDTALNHHVFPEAAIPSVYLLYKTSHYNILY 318
[Close]
BLAST of Ubiquitin thioesterase vs. Ensembl Mouse
Match: Otub2 (OTU domain, ubiquitin aldehyde binding 2 [Source:MGI Symbol;Acc:MGI:1915399])

HSP 1 Score: 145.206 bits (365), Expect = 1.690e-40
Identity = 79/225 (35.11%), Postives = 129/225 (57.33%), Query Frame = 2 
Query:  257 KDFGGHNSYLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVENCQNSGDIQCLTFTPSHIDSQSKDEIFINSYIT---LLYRPGHYDILY 922
            +D   +  Y  K++ L  ++  +R+ +GDGNCF+R+ G+S+LESLL +S      + KER     N L+  G+ E   ++F+  F  +V+ ++ DS +S L ++FN+Q  SD  + FLRLL S++++  A +++ FI+E M +K FC  EVEPM +E D++ I ALSQ+L + + VE                 +D+     +F  + I    LLY+  HY+ILY
Sbjct:  109 RDHPENRIYQRKIQELSKRFTSIRKTKGDGNCFYRALGYSYLESLLGKSRE--ILKFKERVLQTPNDLLAAGFEEHKFRNFFNAFYSVVELVEKDSSVSSLLKVFNDQSSSDRIVQFLRLLTSAFIRNRADFFRHFIDEEMDIKDFCTHEVEPMAMECDHVQITALSQALNIALQVEYVDE-------------MDTALNHHVFPEAAIPSVYLLYKTSHYNILY 318
[Close]
BLAST of Ubiquitin thioesterase vs. UniProt/SwissProt
Match: sp|B2RYG6|OTUB1_RAT (Ubiquitin thioesterase OTUB1 OS=Rattus norvegicus OX=10116 GN=Otub1 PE=1 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 3.507e-54
Identity = 95/217 (43.78%), Postives = 140/217 (64.52%), Query Frame = 2 
Query:  275 NSYLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVENCQNSGDIQCLTFTPSHIDSQSKDEIFINSYITLLYRPGHYDILYE 925
            N Y +K+K L  KY  +R+ R DGNCF+R+FGFS LE+LL   ++    R K      K  L+ QG++EFTI+DF+  F+++++ ++  + ++ L   FN+Q  SDY +V+LRLL S YLQ  + +++ FIE G T+K+FC++EVEPM  ESD+IHIIAL+Q+L V+I VE        +  T  P      S+ +++      LLYRPGHYDILY+
Sbjct:   66 NIYQQKIKDLHKKYSYIRKTRPDGNCFYRAFGFSHLEALL--DDSKELQRFKAVSAKSKEDLVSQGFTEFTIEDFHNTFMDLIEQVEKQTSVADLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEGGRTVKEFCQQEVEPMCKESDHIHIIALAQALSVSIQVEYMDRG---EGGTTNPHVFPEGSEPKVY------LLYRPGHYDILYK 271
[Close]
BLAST of Ubiquitin thioesterase vs. UniProt/SwissProt
Match: sp|Q7TQI3|OTUB1_MOUSE (Ubiquitin thioesterase OTUB1 OS=Mus musculus OX=10090 GN=Otub1 PE=1 SV=2)

HSP 1 Score: 181.415 bits (459), Expect = 3.507e-54
Identity = 95/217 (43.78%), Postives = 140/217 (64.52%), Query Frame = 2 
Query:  275 NSYLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVENCQNSGDIQCLTFTPSHIDSQSKDEIFINSYITLLYRPGHYDILYE 925
            N Y +K+K L  KY  +R+ R DGNCF+R+FGFS LE+LL   ++    R K      K  L+ QG++EFTI+DF+  F+++++ ++  + ++ L   FN+Q  SDY +V+LRLL S YLQ  + +++ FIE G T+K+FC++EVEPM  ESD+IHIIAL+Q+L V+I VE        +  T  P      S+ +++      LLYRPGHYDILY+
Sbjct:   66 NIYQQKIKDLHKKYSYIRKTRPDGNCFYRAFGFSHLEALL--DDSKELQRFKAVSAKSKEDLVSQGFTEFTIEDFHNTFMDLIEQVEKQTSVADLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEGGRTVKEFCQQEVEPMCKESDHIHIIALAQALSVSIQVEYMDRG---EGGTTNPHVFPEGSEPKVY------LLYRPGHYDILYK 271
[Close]
BLAST of Ubiquitin thioesterase vs. UniProt/SwissProt
Match: sp|Q96FW1|OTUB1_HUMAN (Ubiquitin thioesterase OTUB1 OS=Homo sapiens OX=9606 GN=OTUB1 PE=1 SV=2)

HSP 1 Score: 181.03 bits (458), Expect = 4.790e-54
Identity = 95/217 (43.78%), Postives = 140/217 (64.52%), Query Frame = 2 
Query:  275 NSYLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVENCQNSGDIQCLTFTPSHIDSQSKDEIFINSYITLLYRPGHYDILYE 925
            N Y +K+K L  KY  +R+ R DGNCF+R+FGFS LE+LL   ++    R K      K  L+ QG++EFTI+DF+  F+++++ ++  + ++ L   FN+Q  SDY +V+LRLL S YLQ  + +++ FIE G T+K+FC++EVEPM  ESD+IHIIAL+Q+L V+I VE        +  T  P      S+ +++      LLYRPGHYDILY+
Sbjct:   66 NIYQQKIKDLHKKYSYIRKTRPDGNCFYRAFGFSHLEALL--DDSKELQRFKAVSAKSKEDLVSQGFTEFTIEDFHNTFMDLIEQVEKQTSVADLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEGGRTVKEFCQQEVEPMCKESDHIHIIALAQALSVSIQVEYMDRG---EGGTTNPHIFPEGSEPKVY------LLYRPGHYDILYK 271
[Close]
BLAST of Ubiquitin thioesterase vs. UniProt/SwissProt
Match: sp|Q9VL00|OTUBL_DROME (Ubiquitin thioesterase otubain-like OS=Drosophila melanogaster OX=7227 GN=CG4968 PE=2 SV=1)

HSP 1 Score: 172.555 bits (436), Expect = 6.681e-51
Identity = 108/254 (42.52%), Postives = 154/254 (60.63%), Query Frame = 2 
Query:  191 IQNEIKETQXXXXXXXXXXXXXKDFGGHNSYLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDIS------KLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVE--NCQNSGDIQCLTFTPSHIDSQSKDEIFINSYITLLYRPGHYDILYEN 928
            I+ EI +T PL+SE LPL+ L  ++ G   +  K++ L  KY+ +RR R DGNCFFR+F +S+LE L+  SN SA+   K+  E  K  L+Q G+  FT++DF+E F+E++  +  D+         +L +IFN QG+SDY +V+LRL+ S  LQ  A +YQ FIE  +T++ F   EVEPMY ESD+IHIIAL  +L   + VE  +    G ++   F      S+ +        I L+YRPGHYDILY N
Sbjct:   22 IEKEISDTTPLVSEQLPLTCLYAEYSGDEIFTAKIQDLSKKYKFIRRTRPDGNCFFRAFAYSYLEYLI--SNTSAYQEFKKLAEESKEKLVQLGFPSFTLEDFHETFMEVIQRVSPDNAGGHSTVQDELHKIFNEQGYSDYVVVYLRLITSGKLQEEADFYQNFIEGDLTIEAFRHLEVEPMYKESDHIHIIALCTALGAGVRVEYLDRGEGGTVKAHDFPEG---SEPR--------IYLIYRPGHYDILYPN 262
[Close]
BLAST of Ubiquitin thioesterase vs. UniProt/SwissProt
Match: sp|Q8LG98|OTU1_ARATH (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 1 OS=Arabidopsis thaliana OX=3702 GN=OTU1 PE=1 SV=2)

HSP 1 Score: 167.162 bits (422), Expect = 3.029e-48
Identity = 98/230 (42.61%), Postives = 136/230 (59.13%), Query Frame = 2 
Query:  269 GHNSYLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDIS-KLEEIFN---NQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIE--EGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINV-----ENCQNSG-DIQCLTFTPSHIDSQSKDEIFINSYITLLYRPGHYDILY 922
            G    LEK+KIL  +Y G+RR RGDGNCFFRSF FS+LE +L+  + +  DRIK   E  +  L   GY++FT +DF+  FLE +D +   ++ S   +E+ N   +Q  SDY ++F R + +  ++  A +++ FI      T+ QFC+  VEPM  ESD+IHI ALS +L V I V      +C + G  +    F P  I +  KDE     +ITLLYRPGHYDILY
Sbjct:   65 GSPILLEKIKILDSQYIGIRRTRGDGNCFFRSFMFSYLEHILESQDRAEVDRIKVNVEKCRKTLQNLGYTDFTFEDFFALFLEQLDDILQGTEESISYDELVNRSRDQSVSDYIVMFFRFVTAGDIRTRADFFEPFITGLSNATVDQFCKSSVEPMGEESDHIHITALSDALGVAIRVVYLDRSSCDSGGVTVNHHDFVPVGI-TNEKDEEASAPFITLLYRPGHYDILY 293
[Close]
BLAST of Ubiquitin thioesterase vs. TrEMBL
Match: Q5DC72 (Ubiquitin thioesterase OS=Schistosoma japonicum OX=6182 PE=2 SV=1)

HSP 1 Score: 236.884 bits (603), Expect = 2.308e-73
Identity = 120/251 (47.81%), Postives = 167/251 (66.53%), Query Frame = 2 
Query:  257 KDFGGHNSYLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDS-DISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVENCQNSGDIQCLTFTPSHID-------SQSKDEI-------------------FINSYITLLYRPGHYDILYEN 928
            +DF GH+ YLEKLK++   Y+G+RR+R DGNCF+R+FGF+++E LL         R K++C+  K+ LI  GY++FT++DF+EQF+ MVD   +D+  + +LEE+FN+Q +SDYY+VFLRLLVS+Y+Q NA Y+  FI+E  T+ QFCE EVEPM  ESDNIH+ AL+ ++ + I VENCQ SG++  + F P+H D       S S D +                   + N  +TLLYRPGHYDILY N
Sbjct:   18 EDFNGHSVYLEKLKLMKQNYRGIRRLRRDGNCFYRAFGFAYIEYLLSGKLIKEAGRFKKKCDVCKDTLIANGYTQFTVEDFHEQFVGMVDRFTVDNGTLEELEEVFNDQAYSDYYVVFLRLLVSAYIQKNAGYFVNFIDEDKTVSQFCETEVEPMARESDNIHVAALALAVELPIYVENCQQSGELNRIEF-PAHSDLININEGSSSDDSVSAEQIVCSENDSLTNNHQKYNNPPVTLLYRPGHYDILYPN 267
[Close]
BLAST of Ubiquitin thioesterase vs. TrEMBL
Match: C1L832 (Ubiquitin thioesterase OS=Schistosoma japonicum OX=6182 PE=2 SV=1)

HSP 1 Score: 237.654 bits (605), Expect = 2.415e-73
Identity = 120/251 (47.81%), Postives = 167/251 (66.53%), Query Frame = 2 
Query:  257 KDFGGHNSYLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDS-DISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVENCQNSGDIQCLTFTPSHID-------SQSKDEI-------------------FINSYITLLYRPGHYDILYEN 928
            +DF GH+ YLEKLK++   Y+G+RR+R DGNCF+R+FGF+++E LL         R K++C+  K+ LI  GY++FT++DF+EQF+ MVD   +D+  + +LEE+FN+Q +SDYY+VFLRLLVS+Y+Q NA Y+  FI+E  T+ QFCE EVEPM  ESDNIH+ AL+ ++ + I VENCQ SG++  + F P+H D       S S D +                   + N  +TLLYRPGHYDILY N
Sbjct:   44 EDFNGHSVYLEKLKLMKQNYRGIRRLRRDGNCFYRAFGFAYIEYLLSGKLIKEAGRFKKKCDVCKDTLIANGYTQFTVEDFHEQFVGMVDRFTVDNGTLEELEEVFNDQAYSDYYVVFLRLLVSAYIQKNAGYFVNFIDEDKTVSQFCETEVEPMARESDNIHVAALALAVELPIYVENCQQSGELNRIEF-PAHSDLININEGSSSDDSVSAEQIVCSENDSLTNNHQKYNNPPVTLLYRPGHYDILYPN 293
[Close]
BLAST of Ubiquitin thioesterase vs. TrEMBL
Match: C1L834 (Ubiquitin thioesterase OS=Schistosoma japonicum OX=6182 PE=2 SV=1)

HSP 1 Score: 237.654 bits (605), Expect = 2.626e-73
Identity = 120/251 (47.81%), Postives = 167/251 (66.53%), Query Frame = 2 
Query:  257 KDFGGHNSYLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSD-ISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVENCQNSGDIQCLTFTPSHID-------SQSKDEI-------------------FINSYITLLYRPGHYDILYEN 928
            +DF GH+ YLEKLK++   Y+G+RR+R DGNCF+R+FGF+++E LL         R K++C+  K+ LI  GY++FT++DF+EQF+ MVD   +D+  + +LEE+FN+Q +SDYY+VFLRLLVS+Y+Q NA Y+  FI+E  T+ QFCE EVEPM  ESDNIH+ AL+ ++ + I VENCQ SG++  + F P+H D       S S D +                   + N  +TLLYRPGHYDILY N
Sbjct:   46 EDFNGHSVYLEKLKLMKQNYRGIRRLRRDGNCFYRAFGFAYIEYLLSGKLIKEAGRFKKKCDVCKDTLIANGYTQFTVEDFHEQFVGMVDRFTVDNGTLEELEEVFNDQAYSDYYVVFLRLLVSAYIQKNAGYFVNFIDEDKTVSQFCETEVEPMARESDNIHVAALALAVELPIYVENCQQSGELNRIEF-PAHSDLININEGSSSDDSVSAEQIVCSENDSLTNNHQKYNNPPVTLLYRPGHYDILYPN 295
[Close]
BLAST of Ubiquitin thioesterase vs. TrEMBL
Match: C1L833 (Ubiquitin thioesterase OS=Schistosoma japonicum OX=6182 PE=2 SV=1)

HSP 1 Score: 237.269 bits (604), Expect = 3.480e-73
Identity = 120/251 (47.81%), Postives = 167/251 (66.53%), Query Frame = 2 
Query:  257 KDFGGHNSYLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSD-ISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVENCQNSGDIQCLTFTPSHID-------SQSKDEI-------------------FINSYITLLYRPGHYDILYEN 928
            +DF GH+ YLEKLK++   Y+G+RR+R DGNCF+R+FGF+++E LL         R K++C+  K+ LI  GY++FT++DF+EQF+ MVD   +D+  + +LEE+FN+Q +SDYY+VFLRLLVS+Y+Q NA Y+  FI+E  T+ QFCE EVEPM  ESDNIH+ AL+ ++ + I VENCQ SG++  + F P+H D       S S D +                   + N  +TLLYRPGHYDILY N
Sbjct:   46 EDFNGHSVYLEKLKLMKQNYRGIRRLRRDGNCFYRAFGFAYIEYLLSGKLIKEAGRFKKKCDVCKDTLIANGYTQFTVEDFHEQFVGMVDRFTVDNGTLEELEEVFNDQAYSDYYVVFLRLLVSAYIQKNAGYFVNFIDEDKTVSQFCETEVEPMARESDNIHVAALALAVELPIYVENCQQSGELNRIEF-PAHSDLININEGSSSDDSVSAEQIVCSENDSLTNNHQKYNNPPVTLLYRPGHYDILYPN 295
[Close]
BLAST of Ubiquitin thioesterase vs. TrEMBL
Match: Q86ED9 (Ubiquitin thioesterase OS=Schistosoma japonicum OX=6182 PE=2 SV=1)

HSP 1 Score: 234.187 bits (596), Expect = 6.492e-72
Identity = 119/251 (47.41%), Postives = 165/251 (65.74%), Query Frame = 2 
Query:  257 KDFGGHNSYLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSD-ISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVENCQNSGDIQCLTFTPSHID-------SQSKDEI-------------------FINSYITLLYRPGHYDILYEN 928
            +DF GH+ YLEKLK++   Y+G+RR+R DGNCF+R+FGF+++E LL         R K++C+  K+ LI  GY++FT++DF+EQF+ MVD    D+  + +LEE+ N+Q +SDYY+VFLRLLVS+Y+Q NA Y+  FI+E  T+ QFCE EVEPM  ESDNIH+ AL+ ++ + I VENCQ SG++  + F P+H D       S S D +                   + N  +TLLYRPGHYDILY N
Sbjct:   46 EDFNGHSVYLEKLKLMKQNYRGIRRLRRDGNCFYRAFGFAYIEYLLSGKLIKEAGRFKKKCDVCKDTLIANGYTQFTVEDFHEQFVGMVDRFTGDNGTLEELEEVVNDQAYSDYYVVFLRLLVSAYIQKNAGYFVNFIDEDKTVSQFCETEVEPMARESDNIHVAALALAVELPIYVENCQQSGELNRIEF-PAHSDLININEGSSSDDSVSAEQIVCSENDSLTNNHQKYNNPPVTLLYRPGHYDILYPN 295
[Close]
BLAST of Ubiquitin thioesterase vs. Ensembl Cavefish
Match: otub1b (OTU deubiquitinase, ubiquitin aldehyde binding 1 [Source:NCBI gene;Acc:103024331])

HSP 1 Score: 176.407 bits (446), Expect = 2.593e-53
Identity = 109/249 (43.78%), Postives = 155/249 (62.25%), Query Frame = 2 
Query:  188 RIQNEIKETQXXXXXXXXXXXXXKDFGGHNS-YLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVE--NCQNSGDIQCLTFTPSHIDSQSKDEIFINSYITLLYRPGHYDILYE 925
            RIQ EI  T PL+S+   LS L+K++   ++ Y  K+K L  KY  +R+ R DGNCF+R+FGFS LESLL   ++    + K      K  L+ QG++EFTI+DF+  F+++++  +    + +L   FN+Q  SDY +V+LRLL S YLQ    ++Q FIE G ++K+FC++EVEPM  ESD+IHIIAL+Q+L V+I VE  +    G +    F        S+  IF      LLYRPGHYDILY+
Sbjct:   31 RIQQEIATTNPLVSDRQELSVLQKEYAEEDTIYQLKIKDLHKKYSYIRKTRPDGNCFYRAFGFSHLESLL--DDSKELQKFKAVAAKSKLDLVTQGFTEFTIEDFHNTFMDLIELCEKQPALGELLNSFNDQSVSDYVVVYLRLLTSGYLQREHGFFQHFIEGGRSVKEFCQQEVEPMSKESDHIHIIALAQALNVSILVEYMDRGEGGTVNHHVFPEG-----SEPRIF------LLYRPGHYDILYK 266
[Close]
BLAST of Ubiquitin thioesterase vs. Ensembl Cavefish
Match: otub1a (ubiquitin thioesterase OTUB1-like [Source:NCBI gene;Acc:103027164])

HSP 1 Score: 169.474 bits (428), Expect = 1.518e-50
Identity = 102/249 (40.96%), Postives = 152/249 (61.04%), Query Frame = 2 
Query:  188 RIQNEIKETQXXXXXXXXXXXXXKDFGGHNS-YLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVE--NCQNSGDIQCLTFTPSHIDSQSKDEIFINSYITLLYRPGHYDILYE 925
            RIQ EI  +  L+S+   L+ L+K++   ++ Y +K++ L   Y  +R+ R DGNCF+R+FGFS+LESLL   ++    R K      K  L+ QG++EFTI+DF+  F+++++       + +L   FN Q  SDY +V+LRL+ S +LQ    ++Q FIE G T+++FC++EVEPM  ESD+IHIIAL+Q+L V I VE  +    G +    F P   D +          + LLYRPGHYDILY+
Sbjct:   34 RIQQEIATSISLVSDQQDLAILKKEYAEDDTIYQQKIRDLQKTYTFIRKTRPDGNCFYRAFGFSYLESLL--DDSKELHRFKAVAAKSKMDLVSQGFTEFTIEDFHNTFMDLIEVCDKQPSVGELLNSFNEQSVSDYLVVYLRLVTSGFLQREEDFFQHFIEGGRTVREFCQQEVEPMSKESDHIHIIALAQALNVCILVEYMDRGEGGTVNHHIF-PEDGDPK----------VFLLYRPGHYDILYK 269
[Close]
BLAST of Ubiquitin thioesterase vs. Ensembl Cavefish
Match: OTUB2 (OTU deubiquitinase, ubiquitin aldehyde binding 2 [Source:NCBI gene;Acc:103042941])

HSP 1 Score: 114.39 bits (285), Expect = 3.892e-29
Identity = 71/208 (34.13%), Postives = 120/208 (57.69%), Query Frame = 2 
Query:  305 CGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVENCQNSGDIQCLTFTPSHIDSQSKDEIFINSYITLLYRPGHYDILYEN 928
            C ++  +RRV+GDGNCF+R+  F  +E+L+   N S+  RIK++       L++ G  E   ++    FL ++D L+ D     L   FN+   SD  + +LRLL S++LQ +A ++Q F+ E   +K +C +EVE M +E D++ I+ALS++L +++ + +  +S D   +  T   I   S+  I+      LLY+  HYDILY++
Sbjct:  117 CQQFGFIRRVKGDGNCFYRALCFRLVETLMH--NGSSVRRIKDQLLRSNEDLVRAGVEEGEFKELIGTFLRVLDQLEADDCEETLLGFFNDHTTSDSMVKYLRLLTSAHLQNHADFFQHFL-EAPNLKAYCTQEVEAMAMECDHVEILALSEALDISLCIIS-MDSSDGHLVYHT---IPEGSECSIY------LLYKTSHYDILYKH 311
[Close]
BLAST of Ubiquitin thioesterase vs. Ensembl Sea Lamprey
Match: otub1a (OTU deubiquitinase, ubiquitin aldehyde binding 1a [Source:ZFIN;Acc:ZDB-GENE-040718-108])

HSP 1 Score: 174.096 bits (440), Expect = 5.595e-53
Identity = 93/217 (42.86%), Postives = 135/217 (62.21%), Query Frame = 2 
Query:  281 YLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVE--NCQNSGDIQCLTFTPSHIDSQSKDEIFINSYITLLYRPGHYDILYE 925
            Y +K+K LC KY  +R  R DGNCF+R+FGF+ LE+L+        +R K      K+ L+  G+ EFTI+DF+  FL++++ ++  + +  +   FN Q  SDY +V+LRLL S YLQ  + +++ F+E G T+K+FC +EVEPM  ESD+IHIIAL+Q+L V+I VE  +    G +       +H D     E F    + LLYRPGHYDILY+
Sbjct:   66 YQKKIKDLCNKYSHIRMTRRDGNCFYRAFGFAHLEALMDNPK-DLQNRFKTVAAKSKDELVSLGFPEFTIEDFHSTFLDIIEMVEKQASLEDVRGSFNEQSTSDYVVVYLRLLTSGYLQKESIFFEHFVEGGRTVKEFCSQEVEPMAKESDHIHIIALAQALGVSIRVEYMDRGEGGSV-------NHHDFPEGTEPF----VYLLYRPGHYDILYK 270
[Close]
BLAST of Ubiquitin thioesterase vs. Ensembl Nematostella
Match: EDO44100 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RWX6])

HSP 1 Score: 169.859 bits (429), Expect = 5.905e-52
Identity = 87/207 (42.03%), Postives = 130/207 (62.80%), Query Frame = 2 
Query:  302 LCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVENCQNSGDIQCLTFTPSHIDSQSKDEIFINSYITLLYRPGHYDILY 922
            L  +Y  +RR RGDGNCFFR+FGFS+ E LL  ++   + R K+     K+ L++  +  FTI+DF+E F+E++D +  ++   +L + F ++G S+Y +V+LRLL S+ LQ NA +++ FIE   T+K+FC +EVEPM  ESD+IHII L+++L V I VE    +GD          ++     E      + LLYRPGHYD+LY
Sbjct:    3 LSAQYSHVRRTRGDGNCFFRAFGFSYFEKLL--TDEKEYQRFKDLAARSKDELVELNFPAFTIEDFHEVFMEVLDMMGNNATEDQLIQKFQDEGISNYLVVYLRLLTSAQLQRNAVFFENFIEGERTVKEFCNQEVEPMGKESDHIHIIGLTEALGVCIRVEYVDRTGD---------DVNHHDFPEDGSTPIVHLLYRPGHYDVLY 198
[Close]
BLAST of Ubiquitin thioesterase vs. Ensembl Nematostella
Match: EDO44101 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RWX7])

HSP 1 Score: 59.3066 bits (142), Expect = 8.341e-12
Identity = 33/74 (44.59%), Postives = 42/74 (56.76%), Query Frame = 2 
Query:  701 KEVEPMYIESDNIHIIALSQSLLVNINVENCQNSGDIQCLTFTPSHIDSQSKDEIFINSYITLLYRPGHYDILY 922
            KEVEPM  ESD+IHII L+++L V I VE    +GD          ++     E      + LLYRPGHYD+LY
Sbjct:    1 KEVEPMGKESDHIHIIGLTEALGVCIRVEYVDRTGD---------DVNHHDFPEDGSTPIVHLLYRPGHYDVLY 65
[Close]
BLAST of Ubiquitin thioesterase vs. Ensembl Medaka
Match: otub1b (OTU deubiquitinase, ubiquitin aldehyde binding 1 [Source:NCBI gene;Acc:101167904])

HSP 1 Score: 171.785 bits (434), Expect = 1.368e-51
Identity = 109/249 (43.78%), Postives = 153/249 (61.45%), Query Frame = 2 
Query:  188 RIQNEIKETQXXXXXXXXXXXXXKDFGGHN-SYLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVE--NCQNSGDIQCLTFTPSHIDSQSKDEIFINSYITLLYRPGHYDILYE 925
            RIQ EI  T PL+SE   LS L+K++   +  Y  K+K L  KY  +R+ R DGNCF+R+FGF+ LESLL+ S      R K      K  L+ +G++EFTI+DF+  F+++++  +    + +L   FN+Q  SDY +V+LRLL S YLQ    ++  FIE G ++K+FC++EVEPM  ESD+IHIIAL+Q+L V+I VE  +    G +    F P   D +          I LLYRPGHYDILY+
Sbjct:   31 RIQQEIANTNPLVSERQELSVLQKEYAEDDMVYQLKIKDLHKKYSHIRKTRPDGNCFYRAFGFAHLESLLEDS--KELQRFKAVAAKSKLDLVNEGFTEFTIEDFHNTFMDLIELCEKQPSLQELLSSFNDQNVSDYVVVYLRLLTSGYLQRENGFFLNFIEGGRSIKEFCQQEVEPMSKESDHIHIIALAQALNVSILVEYMDRGEGGTVNHHVF-PEGGDPR----------IFLLYRPGHYDILYK 266
[Close]
BLAST of Ubiquitin thioesterase vs. Ensembl Medaka
Match: OTUB2 (OTU deubiquitinase, ubiquitin aldehyde binding 2 [Source:NCBI gene;Acc:101170908])

HSP 1 Score: 102.064 bits (253), Expect = 2.544e-25
Identity = 68/212 (32.08%), Postives = 118/212 (55.66%), Query Frame = 2 
Query:  290 KLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVENCQNSGDIQCLTFTPSHIDSQSKDEIFINSYITLLYRPGHYDILYE 925
            K K L G++ G+R++ GDGNCF+R+F F++LES+       A +R K++       L   G+ E +     +  + +V+  + D   S L ++FN+   SD  + +LRLL S++LQ  A ++  F+ E   ++ +C +EVE M +E D++ I+ALSQ+L V +++ + +  G+ Q L        +Q          + LLY+  HY+ILY+
Sbjct:   25 KQKDLDGEFFGVRKILGDGNCFYRAFCFAYLESIFHIPR--ALERFKQKIIQSGQVLAVAGFEESSFIHHLDTVVNVVEECQADEQESTLLKLFNDSAVSDGVVQYLRLLTSAHLQNFADFFCNFV-EAPDLQVYCRQEVEVMAMECDHVDILALSQALDVGLHIVSLE--GEQQQLVHYIIPEGAQPS--------LHLLYQSSHYNILYQ 223
[Close]
BLAST of Ubiquitin thioesterase vs. Ensembl Medaka
Match: OTUB2 (OTU deubiquitinase, ubiquitin aldehyde binding 2 [Source:NCBI gene;Acc:101170908])

HSP 1 Score: 97.4413 bits (241), Expect = 8.028e-24
Identity = 56/165 (33.94%), Postives = 98/165 (59.39%), Query Frame = 2 
Query:  290 KLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINV 784
            K K L G++ G+R++ GDGNCF+R+F F++LES+       A +R K++       L   G+ E +     +  + +V+  + D   S L ++FN+   SD  + +LRLL S++LQ  A ++  F+ E   ++ +C +EVE M +E D++ I+ALSQ+L V +++
Sbjct:   25 KQKDLDGEFFGVRKILGDGNCFYRAFCFAYLESIFHIPR--ALERFKQKIIQSGQVLAVAGFEESSFIHHLDTVVNVVEECQADEQESTLLKLFNDSAVSDGVVQYLRLLTSAHLQNFADFFCNFV-EAPDLQVYCRQEVEVMAMECDHVDILALSQALDVGLHI 186
[Close]
BLAST of Ubiquitin thioesterase vs. Planmine SMEST
Match: SMESG000043359.1 (SMESG000043359.1)

HSP 1 Score: 482.641 bits (1241), Expect = 7.392e-174
Identity = 249/249 (100.00%), Postives = 249/249 (100.00%), Query Frame = 2 
Query:  188 RIQNEIKETQXXXXXXXXXXXXXKDFGGHNSYLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVENCQNSGDIQCLTFTPSHIDSQSKDEIFINSYITLLYRPGHYDILYENDL 934
            RIQNEIKETQPLISELLPLSSLEKDFGGHNSYLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVENCQNSGDIQCLTFTPSHIDSQSKDEIFINSYITLLYRPGHYDILYENDL
Sbjct:   16 RIQNEIKETQPLISELLPLSSLEKDFGGHNSYLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVENCQNSGDIQCLTFTPSHIDSQSKDEIFINSYITLLYRPGHYDILYENDL 264
[Close]
BLAST of Ubiquitin thioesterase vs. Planmine SMEST
Match: SMESG000043359.1 (SMESG000043359.1)

HSP 1 Score: 440.269 bits (1131), Expect = 2.340e-157
Identity = 234/249 (93.98%), Postives = 234/249 (93.98%), Query Frame = 2 
Query:  188 RIQNEIKETQXXXXXXXXXXXXXKDFGGHNSYLEKLKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVENCQNSGDIQCLTFTPSHIDSQSKDEIFINSYITLLYRPGHYDILYENDL 934
            RIQNEIKETQPLISELLPLSSLEKDFGGHNSYLEKLKILCGKYQGMRRVR               ESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVENCQNSGDIQCLTFTPSHIDSQSKDEIFINSYITLLYRPGHYDILYENDL
Sbjct:   16 RIQNEIKETQPLISELLPLSSLEKDFGGHNSYLEKLKILCGKYQGMRRVR---------------ESLLKQSNASAFDRIKERCEFYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIFNNQGFSDYYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIHIIALSQSLLVNINVENCQNSGDIQCLTFTPSHIDSQSKDEIFINSYITLLYRPGHYDILYENDL 249
The following BLAST results are available for this feature:
BLAST of Ubiquitin thioesterase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
OTUB14.326e-5543.78OTU deubiquitinase, ubiquitin aldehyde binding 1 [... [more]
OTUB19.974e-5543.78OTU deubiquitinase, ubiquitin aldehyde binding 1 [... [more]
OTUB19.974e-5543.78OTU deubiquitinase, ubiquitin aldehyde binding 1 [... [more]
OTUB11.858e-5443.78OTU deubiquitinase, ubiquitin aldehyde binding 1 [... [more]
OTUB14.983e-5444.24OTU deubiquitinase, ubiquitin aldehyde binding 1 [... [more]
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BLAST of Ubiquitin thioesterase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
otub-14.154e-3632.59Ubiquitin thioesterase otubain-like [Source:UniPr... [more]
otub-14.154e-3632.59Ubiquitin thioesterase otubain-like [Source:UniPr... [more]
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BLAST of Ubiquitin thioesterase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
CG49686.665e-5242.52gene:FBgn0032214 transcript:FBtr0079987[more]
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BLAST of Ubiquitin thioesterase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
otub1a3.641e-5243.78OTU deubiquitinase, ubiquitin aldehyde binding 1a ... [more]
otub1b3.554e-5142.17OTU deubiquitinase, ubiquitin aldehyde binding 1b ... [more]
otub1b1.511e-4543.78OTU deubiquitinase, ubiquitin aldehyde binding 1b ... [more]
otub1a4.230e-3339.88OTU deubiquitinase, ubiquitin aldehyde binding 1a ... [more]
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BLAST of Ubiquitin thioesterase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3
Match NameE-valueIdentityDescription
OTUB13.866e-5645.12OTU deubiquitinase, ubiquitin aldehyde binding 1 [... [more]
mcm32.138e-3336.23minichromosome maintenance complex component 3 [So... [more]
mcm32.547e-3336.23minichromosome maintenance complex component 3 [So... [more]
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BLAST of Ubiquitin thioesterase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Otub12.582e-5543.78OTU domain, ubiquitin aldehyde binding 1 [Source:M... [more]
Otub15.014e-5543.78OTU domain, ubiquitin aldehyde binding 1 [Source:M... [more]
Otub22.188e-4135.11OTU domain, ubiquitin aldehyde binding 2 [Source:M... [more]
Otub21.690e-4035.11OTU domain, ubiquitin aldehyde binding 2 [Source:M... [more]
Otub21.690e-4035.11OTU domain, ubiquitin aldehyde binding 2 [Source:M... [more]
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BLAST of Ubiquitin thioesterase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|B2RYG6|OTUB1_RAT3.507e-5443.78Ubiquitin thioesterase OTUB1 OS=Rattus norvegicus ... [more]
sp|Q7TQI3|OTUB1_MOUSE3.507e-5443.78Ubiquitin thioesterase OTUB1 OS=Mus musculus OX=10... [more]
sp|Q96FW1|OTUB1_HUMAN4.790e-5443.78Ubiquitin thioesterase OTUB1 OS=Homo sapiens OX=96... [more]
sp|Q9VL00|OTUBL_DROME6.681e-5142.52Ubiquitin thioesterase otubain-like OS=Drosophila ... [more]
sp|Q8LG98|OTU1_ARATH3.029e-4842.61OVARIAN TUMOR DOMAIN-containing deubiquitinating e... [more]
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BLAST of Ubiquitin thioesterase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
Q5DC722.308e-7347.81Ubiquitin thioesterase OS=Schistosoma japonicum OX... [more]
C1L8322.415e-7347.81Ubiquitin thioesterase OS=Schistosoma japonicum OX... [more]
C1L8342.626e-7347.81Ubiquitin thioesterase OS=Schistosoma japonicum OX... [more]
C1L8333.480e-7347.81Ubiquitin thioesterase OS=Schistosoma japonicum OX... [more]
Q86ED96.492e-7247.41Ubiquitin thioesterase OS=Schistosoma japonicum OX... [more]
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BLAST of Ubiquitin thioesterase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
otub1b2.593e-5343.78OTU deubiquitinase, ubiquitin aldehyde binding 1 [... [more]
otub1a1.518e-5040.96ubiquitin thioesterase OTUB1-like [Source:NCBI gen... [more]
OTUB23.892e-2934.13OTU deubiquitinase, ubiquitin aldehyde binding 2 [... [more]
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BLAST of Ubiquitin thioesterase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
otub1a5.595e-5342.86OTU deubiquitinase, ubiquitin aldehyde binding 1a ... [more]
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BLAST of Ubiquitin thioesterase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ubiquitin thioesterase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO441005.905e-5242.03Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO441018.341e-1244.59Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Ubiquitin thioesterase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
otub1b1.368e-5143.78OTU deubiquitinase, ubiquitin aldehyde binding 1 [... [more]
OTUB22.544e-2532.08OTU deubiquitinase, ubiquitin aldehyde binding 2 [... [more]
OTUB28.028e-2433.94OTU deubiquitinase, ubiquitin aldehyde binding 2 [... [more]
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BLAST of Ubiquitin thioesterase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000043359.17.392e-174100.00SMESG000043359.1[more]
SMESG000043359.12.340e-15793.98SMESG000043359.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30000494 ID=SMED30000494|Name=Ubiquitin thioesterase|organism=Schmidtea mediterranea sexual|type=transcript|length=960bp
TAATACTGTATATCATAATAATGTATTAAAAAACTGTGAATAAATAACCG
AATTGTAATAAATTTTATTGATCCTTGTTTAAATATTGTTCTTTTATAAT
AATATAACTTTCATTTCACTACTTAGTTTTGCAATTTTGTCGATGTCAGC
TCAGGAAGCTTATGAAGCCACACAAGCTCAACAAGAGAGAATTCAAAATG
AGATCAAAGAAACACAGCCTCTAATCTCTGAACTCCTTCCTTTGTCGTCT
TTGGAAAAAGATTTTGGTGGTCATAATTCGTATTTAGAAAAATTAAAAAT
ATTGTGTGGTAAATACCAAGGAATGAGACGAGTACGAGGTGACGGGAATT
GTTTTTTTCGGTCTTTTGGATTTAGTTTTTTAGAATCATTACTAAAACAA
TCCAATGCATCGGCATTTGATCGTATTAAAGAAAGATGTGAATTCTACAA
AAATTATCTAATCCAACAAGGATACTCCGAATTTACAATTCAAGATTTTT
ATGAACAATTTCTAGAAATGGTGGATTGCCTAAAAATGGATAGTGATATT
AGTAAATTGGAAGAAATTTTCAACAATCAAGGATTTTCCGATTATTATAT
TGTATTTCTGAGATTACTCGTTTCAAGTTATTTACAAATGAATGCTGCAT
ATTATCAAGCTTTTATTGAAGAAGGAATGACAATGAAGCAATTCTGTGAA
AAAGAAGTTGAGCCGATGTACATTGAAAGTGACAATATCCATATAATAGC
ACTAAGTCAATCTTTACTTGTTAATATAAATGTGGAAAATTGTCAAAATT
CTGGAGACATTCAATGCCTTACATTTACTCCTAGTCACATTGATTCCCAG
TCTAAAGATGAAATTTTCATTAATTCATATATTACATTGCTCTATCGGCC
CGGTCACTATGACATATTATATGAAAATGATCTGTAATTGTATTTGTTTT
GGAAATGAAT
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protein sequence of SMED30000494-orf-1

>SMED30000494-orf-1 ID=SMED30000494-orf-1|Name=SMED30000494-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=265bp
MSAQEAYEATQAQQERIQNEIKETQPLISELLPLSSLEKDFGGHNSYLEK
LKILCGKYQGMRRVRGDGNCFFRSFGFSFLESLLKQSNASAFDRIKERCE
FYKNYLIQQGYSEFTIQDFYEQFLEMVDCLKMDSDISKLEEIFNNQGFSD
YYIVFLRLLVSSYLQMNAAYYQAFIEEGMTMKQFCEKEVEPMYIESDNIH
IIALSQSLLVNINVENCQNSGDIQCLTFTPSHIDSQSKDEIFINSYITLL
YRPGHYDILYENDL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
PLANA:0002056medial muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR042467Peptidase_C65_otubain_sub2
IPR042468Peptidase_C65_otubain_sub1
IPR030298OTUB1
IPR038765Papain-like_cys_pep_sf
IPR019400Peptidase_C65_otubain
IPR016615Otubain
IPR003323OTU_dom
Vocabulary: molecular function
TermDefinition
GO:0016787hydrolase activity
GO:0004843thiol-dependent ubiquitin-specific protease activity
GO:0008233peptidase activity
GO:0008234cysteine-type peptidase activity
GO:0019784NEDD8-specific protease activity
GO:0036459thiol-dependent ubiquitinyl hydrolase activity
GO:0043130ubiquitin binding
Vocabulary: biological process
TermDefinition
GO:0016579protein deubiquitination
GO:0006508proteolysis
GO:0071108protein K48-linked deubiquitination
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR042467Peptidase C65, otubain, subdomain 2GENE3DG3DSA:1.20.1300.20coord: 5..150
e-value: 2.0E-61
score: 209.4
IPR019400Peptidase C65, otubainPFAMPF10275Peptidase_C65coord: 1..201
e-value: 5.1E-59
score: 199.7
IPR019400Peptidase C65, otubainPANTHERPTHR12931UBIQUITIN THIOLESTERASE PROTEIN OTUBcoord: 1..200
IPR042468Peptidase C65, otubain, subdomain 1GENE3DG3DSA:3.30.200.60coord: 1..200
e-value: 2.0E-61
score: 209.4
IPR030298Ubiquitin thioesterase OTUB1PANTHERPTHR12931:SF19UBIQUITIN THIOESTERASE OTUB1coord: 1..200
IPR003323OTU domainPROSITEPS50802OTUcoord: 1..202
score: 16.64
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILYSSF54001Cysteine proteinasescoord: 2..201