SMED30000484

Overview


Name	SMED30000484
Smed ID	SMED30000484
Length (bp)	1177

Neoblast Clusters

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of SMED30000484 (SMED30000484) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30000484 (SMED30000484) in cells (dots) of each of the 12 neoblast clusters.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30000484

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 2

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication	Specimen	Lifecycle	Evidence
X1 cell	SMED30000484		SmedASXL_016522	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
X2 cell	SMED30000484		SmedASXL_016522	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015

Note: Hover over icons to view figure legend

Alignments

SMED30000484 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.004104:42975..44126 -	4324

Homology

[Close]

BLAST of SMED30000484 vs. Ensembl Human
Match: TRAF2 (TNF receptor associated factor 2 [Source:HGNC Symbol;Acc:HGNC:12032])

HSP 1 Score: 83.5741 bits (205), Expect = 7.370e-17
Identity = 39/86 (45.35%), Postives = 57/86 (66.28%), Query Frame = -3

Query:   63 YIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCAND 320
            +IWKI+ F +K Q+A      +I S  FY+ R GYKM L++YL+G+G  +GTH+S+FF + KG  D L  WP++ K TL +L  N+
Sbjct:  354 FIWKISDFARKRQEAVAGRIPAIFSPAFYTSRYGYKMCLRIYLNGDGTGRGTHLSLFFVVMKGPNDALLRWPFNQKVTLMLLDQNN 439

[Close]

BLAST of SMED30000484 vs. Ensembl Human
Match: TRAF5 (TNF receptor associated factor 5 [Source:HGNC Symbol;Acc:HGNC:12035])

HSP 1 Score: 82.0333 bits (201), Expect = 3.210e-16
Identity = 39/98 (39.80%), Postives = 61/98 (62.24%), Query Frame = -3

Query:   39 IWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCANDISA-----ETFKP 317
            IWK+T +  K ++A D   +SI S  FY+ R GY++  + YL+G+G  +G+H+S++F + +G+FD L  WP+  + TL +L   D S      ETFKP
Sbjct:  407 IWKVTDYKMKKREAVDGHTVSIFSQSFYTSRCGYRLCARAYLNGDGSGRGSHLSLYFVVMRGEFDSLLQWPFRQRVTLMLL---DQSGKKNIMETFKP 501

[Close]

Query:   39 IWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCANDISA-----ETFKP 317
            IWK+T +  K ++A D   +SI S  FY+ R GY++  + YL+G+G  +G+H+S++F + +G+FD L  WP+  + TL +L   D S      ETFKP
Sbjct:  407 IWKVTDYKMKKREAVDGHTVSIFSQSFYTSRCGYRLCARAYLNGDGSGRGSHLSLYFVVMRGEFDSLLQWPFRQRVTLMLL---DQSGKKNIMETFKP 501

[Close]

Query:   39 IWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCANDISA-----ETFKP 317
            IWK+T +  K ++A D   +SI S  FY+ R GY++  + YL+G+G  +G+H+S++F + +G+FD L  WP+  + TL +L   D S      ETFKP
Sbjct:  407 IWKVTDYKMKKREAVDGHTVSIFSQSFYTSRCGYRLCARAYLNGDGSGRGSHLSLYFVVMRGEFDSLLQWPFRQRVTLMLL---DQSGKKNIMETFKP 501

[Close]

BLAST of SMED30000484 vs. Ensembl Human
Match: TRAF3 (TNF receptor associated factor 3 [Source:HGNC Symbol;Acc:HGNC:12033])

HSP 1 Score: 78.9518 bits (193), Expect = 2.148e-15
Identity = 35/81 (43.21%), Postives = 54/81 (66.67%), Query Frame = -3

Query:   75 IWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAIL 317
            IWKI  + ++ Q+A     +S+ S PFY+   GYKM  +VYL+G+G  KGTH+S+FF + +G++D L  WP+  K TL ++
Sbjct:  336 IWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLM 416

[Close]

BLAST of SMED30000484 vs. Ensembl Celegans
Match: trf-1 (TNF Receptor Associated Factor (TRAF) homolog; TRF-1 [Source:UniProtKB/TrEMBL;Acc:G5EG80])

HSP 1 Score: 62.003 bits (149), Expect = 2.901e-10
Identity = 63/258 (24.42%), Postives = 97/258 (37.60%), Query Frame = -3

Query:   75 CYEMRIKCEHCNKTKTWIKIQNEHKNIKDEKVCKKFKRKCPNQYGL---EKVELKEHWGKYNKRKVDCPLRKWDAKKKTVKEEMPQHLQMKIEEHA*I*MTALRENKLKIDDIVIEVESHKILMTEIFDTREAFELARQRTTDTXXXXXXXXXXXXXXXXXXXXXIPTAGELPVTPYIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAIL 839
            C   R  C  CNKT     I++  +  +  K C +    CP Q GL    ++E++ H          CP   +       KE + QHL  +   H                           L  EI D +  +EL  +            M    +   +++    T  E+     IWKI    ++  +A+     +I S+PF S R GYKM     L G+G   G  IS++  L KG+FD    WP+     +++L
Sbjct:  130 CELNRAVCSFCNKT-----IRDSDRE-RHPKTCPQVIISCPFQCGLTDRPRLEIEAHCPSCPNVDNVCPFVPYGCTFAGGKESIQQHLSDEPVRHL------------------------MYLCDEITDLKGTYELMER-----------DMGSFNDRQTRILSAAETCTEMFGPQLIWKIDKLQQRTNEAKSGADTTIFSVPFMSHRFGYKMMACACLFGDGSSAGKSISLYVLLLKGEFDPTLEWPFHRAIKISLL 346

[Close]

BLAST of SMED30000484 vs. Ensembl Fly
Match: Traf4 (gene:FBgn0026319 transcript:FBtr0334401)

HSP 1 Score: 71.633 bits (174), Expect = 2.816e-13
Identity = 29/81 (35.80%), Postives = 52/81 (64.20%), Query Frame = -3

Query:   75 IWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAIL 317
            +WKIT ++ K  +AR +  + + S PFY+ + GYK++  ++L+GNG  + TH+SV+  +  G++D L  WP+S+  T  + 
Sbjct:  325 LWKITDWSAKMAEARGKDGLELVSPPFYTSQYGYKLQASMFLNGNGPGENTHVSVYIKVLPGEYDALLKWPFSHSITFTLF 405

[Close]

BLAST of SMED30000484 vs. Ensembl Fly
Match: Traf4 (gene:FBgn0026319 transcript:FBtr0077429)

HSP 1 Score: 71.2478 bits (173), Expect = 3.760e-13
Identity = 29/81 (35.80%), Postives = 52/81 (64.20%), Query Frame = -3

Query:   75 IWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAIL 317
            +WKIT ++ K  +AR +  + + S PFY+ + GYK++  ++L+GNG  + TH+SV+  +  G++D L  WP+S+  T  + 
Sbjct:  334 LWKITDWSAKMAEARGKDGLELVSPPFYTSQYGYKLQASMFLNGNGPGENTHVSVYIKVLPGEYDALLKWPFSHSITFTLF 414

[Close]

BLAST of SMED30000484 vs. Ensembl Zebrafish
Match: traf4a (tnf receptor-associated factor 4a [Source:ZFIN;Acc:ZDB-GENE-040308-1])

HSP 1 Score: 86.6557 bits (213), Expect = 4.500e-18
Identity = 64/272 (23.53%), Postives = 113/272 (41.54%), Query Frame = -3

Query:   39 CEHCNKTKTWIKIQNEHKNIKDEKVCKKFKRKCPNQYGLEKVELKEHWGKYNKRK-----VDCPLRKWDAKKKTVKEEMPQHLQMKIEEHA*I*MTALRENKLKIDDIVIEVESHKILMTEIFDTREAFELARQRTTDTXXXXXXXXXXXXXXXXXXXXXIPTAGELPVTPYIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCANDIS-------AETFKP 818
            C++C K   +  IQN   +      C +F  +CPNQ G   +  +E    + K       V CP ++   K +  K  + +HL+   + H  +  + +   + +I ++  E+E   +    +                                                  IWK+T +++K Q+A+        S PFY+ R GYK+++  +L+GNG  +G+H+S++  +  G++D L  WP+S K T +IL  +D S        ETF P
Sbjct:  195 CKYCGKEFVYDTIQNHQYH------CPRFPVQCPNQCGTPNI-AREDLANHVKESCGSALVLCPFKEAGCKHRCPKMAIGRHLEESTKSHLTMMCSLVGRQRQEILELRREMEELSVSHDGVL-------------------------------------------------IWKLTDYSRKLQEAKLRNNHEFFSPPFYTHRYGYKLQVSAFLNGNGSGEGSHLSIYIRVLPGEYDNLLEWPFSYKVTFSILDQSDPSLSKPQHITETFNP 410

[Close]

Query:   39 CEHCNKTKTWIKIQNEHKNIKDEKVCKKFKRKCPNQYGLEKVELKEHWGKYNKRK-----VDCPLRKWDAKKKTVKEEMPQHLQMKIEEHA*I*MTALRENKLKIDDIVIEVESHKILMTEIFDTREAFELARQRTTDTXXXXXXXXXXXXXXXXXXXXXIPTAGELPVTPYIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCANDIS-------AETFKP 818
            C++C K   +  IQN   +      C +F  +CPNQ G   +  +E    + K       V CP ++   K +  K  + +HL+   + H  +  + +   + +I ++  E+E   +    +                                                  IWK+T +++K Q+A+        S PFY+ R GYK+++  +L+GNG  +G+H+S++  +  G++D L  WP+S K T +IL  +D S        ETF P
Sbjct:  195 CKYCGKEFVYDTIQNHQYH------CPRFPVQCPNQCGTPNI-AREDLANHVKESCGSALVLCPFKEAGCKHRCPKMAIGRHLEESTKSHLTMMCSLVGRQRQEILELRREMEELSVSHDGVL-------------------------------------------------IWKLTDYSRKLQEAKLRNNHEFFSPPFYTHRYGYKLQVSAFLNGNGSGEGSHLSIYIRVLPGEYDNLLEWPFSYKVTFSILDQSDPSLSKPQHITETFNP 410

[Close]

BLAST of SMED30000484 vs. Ensembl Zebrafish
Match: traf2b (Tnf receptor-associated factor 2b [Source:ZFIN;Acc:ZDB-GENE-030131-5345])

HSP 1 Score: 84.3445 bits (207), Expect = 3.053e-17
Identity = 40/96 (41.67%), Postives = 62/96 (64.58%), Query Frame = -3

Query:   39 YIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCANDIS--AETFKP 320
            +IWKI  F+++ Q A      ++ S  FYS + GYKM L++YL+G+G  +G+H+S+FF + KGK+D L  WP+S K TL +L  N+     + F+P
Sbjct:  385 FIWKIAEFSRRRQDAVAGRAPAMFSPAFYSSKYGYKMCLRLYLNGDGTGRGSHLSLFFVVMKGKYDALLKWPFSQKVTLMLLDQNNREHIIDAFRP 480

[Close]

Query:   39 YIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCANDIS--AETFKP 320
            +IWKI  F+++ Q A      ++ S  FYS + GYKM L++YL+G+G  +G+H+S+FF + KGK+D L  WP+S K TL +L  N+     + F+P
Sbjct:  385 FIWKIAEFSRRRQDAVAGRAPAMFSPAFYSSKYGYKMCLRLYLNGDGTGRGSHLSLFFVVMKGKYDALLKWPFSQKVTLMLLDQNNREHIIDAFRP 480

[Close]

BLAST of SMED30000484 vs. Ensembl Zebrafish
Match: traf2b (Tnf receptor-associated factor 2b [Source:ZFIN;Acc:ZDB-GENE-030131-5345])

HSP 1 Score: 84.3445 bits (207), Expect = 3.250e-17
Identity = 40/96 (41.67%), Postives = 62/96 (64.58%), Query Frame = -3

Query:   39 YIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCANDIS--AETFKP 320
            +IWKI  F+++ Q A      ++ S  FYS + GYKM L++YL+G+G  +G+H+S+FF + KGK+D L  WP+S K TL +L  N+     + F+P
Sbjct:  398 FIWKIAEFSRRRQDAVAGRAPAMFSPAFYSSKYGYKMCLRLYLNGDGTGRGSHLSLFFVVMKGKYDALLKWPFSQKVTLMLLDQNNREHIIDAFRP 493

[Close]

BLAST of SMED30000484 vs. Ensembl Xenopus
Match: hspb6 (heat shock protein family B (small) member 6 [Source:Xenbase;Acc:XB-GENE-876273])

HSP 1 Score: 81.6481 bits (200), Expect = 1.705e-16
Identity = 38/96 (39.58%), Postives = 60/96 (62.50%), Query Frame = -3

Query:   39 YIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCAN--DISAETFKP 320
            ++WKI+  T K   A     IS+ S  FY+ + GYK+ L++YL+G+G  KGTH+S+FF + KG++D L  WP+ +K T  ++  N  D   + F+P
Sbjct:  232 FLWKISDLTNKSHDAVTGRAISLYSPAFYTAKYGYKVCLRIYLNGDGAGKGTHVSLFFTVMKGEYDALLPWPFKHKVTFVLVDHNNRDSVFDAFRP 327

[Close]

BLAST of SMED30000484 vs. Ensembl Xenopus
Match: TRAF2 (TNF receptor associated factor 2 [Source:NCBI gene;Acc:100494415])

HSP 1 Score: 82.0333 bits (201), Expect = 2.037e-16
Identity = 40/96 (41.67%), Postives = 61/96 (63.54%), Query Frame = -3

Query:   39 YIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCANDIS--AETFKP 320
            +IWKI+ F KK Q A +    ++ S PFY+ + GYKM L++YL+G+G  + TH+S+FF + KG  D L  WP++ K TL +L  N+     + F+P
Sbjct:  307 FIWKISEFGKKRQDAINGRCPAMFSPPFYTNKYGYKMCLRIYLNGDGTGRNTHLSLFFVVMKGNNDALLRWPFNQKVTLMLLDQNNREHVIDAFRP 402

[Close]

BLAST of SMED30000484 vs. Ensembl Xenopus
Match: TRAF2 (TNF receptor associated factor 2 [Source:NCBI gene;Acc:100494415])

HSP 1 Score: 82.0333 bits (201), Expect = 2.127e-16
Identity = 40/96 (41.67%), Postives = 61/96 (63.54%), Query Frame = -3

Query:   39 YIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCANDIS--AETFKP 320
            +IWKI+ F KK Q A +    ++ S PFY+ + GYKM L++YL+G+G  + TH+S+FF + KG  D L  WP++ K TL +L  N+     + F+P
Sbjct:  334 FIWKISEFGKKRQDAINGRCPAMFSPPFYTNKYGYKMCLRIYLNGDGTGRNTHLSLFFVVMKGNNDALLRWPFNQKVTLMLLDQNNREHVIDAFRP 429

[Close]

BLAST of SMED30000484 vs. Ensembl Xenopus
Match: sytl2 (synaptotagmin like 2 [Source:Xenbase;Acc:XB-GENE-945155])

HSP 1 Score: 79.337 bits (194), Expect = 1.706e-15
Identity = 66/274 (24.09%), Postives = 115/274 (41.97%), Query Frame = -3

Query:   39 CEHCNKTKTWIKIQNEHKNIKDEKVCKKFKRKCPNQYGLEKV---ELKEHW-GKYNKRKVDCPLRKWDAKKKTVKEEMPQHLQMKIEEHA*I*MTALRENKLKIDDIVIEVESHKILMTEIFDTREAFELARQRTTDTXXXXXXXXXXXXXXXXXXXXXIPTAGELPVTP---YIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCANDIS-------AETFKP 818
            C +CNK   +  IQ+          C ++   CPNQ G+  +   +L  H     +   V CP +    K +  K  + +HL+  +  H  + M++L   + +                EI + R+  E                                   EL V+     IWKI+ +++K Q+A+        S PFY+ + GYK+++  +L+GNG  +G+++SV+  +  G++D L  WP+S + T ++L  +D S        ETF P
Sbjct:  195 CPYCNKEFVFDTIQSHQYQ------CPRYPTPCPNQCGIASIAREDLSNHLKDSCSSALVLCPFKDSGCKHRGPKITISRHLEENMRAHLSM-MSSLVSRQRQ----------------EILELRKQVE-----------------------------------ELSVSSEGVLIWKISDYSRKVQEAKVRGNFESFSPPFYTHKYGYKLQVSAFLNGNGSGEGSYLSVYIRVLPGEYDNLLEWPFSYRVTFSLLDQSDPSLSKPQHITETFNP 410

[Close]

BLAST of SMED30000484 vs. Ensembl Xenopus
Match: ssh2 (slingshot protein phosphatase 2 [Source:Xenbase;Acc:XB-GENE-993093])

HSP 1 Score: 77.7962 bits (190), Expect = 5.318e-15
Identity = 34/81 (41.98%), Postives = 54/81 (66.67%), Query Frame = -3

Query:   75 IWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAIL 317
            IWKI  + ++ Q+A     +S+ S PFY+   GYKM  +VYL+G+G  KGTH+S+FF + +G++D L  WP+  K +L ++
Sbjct:  387 IWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVSLMLM 467

[Close]

BLAST of SMED30000484 vs. Ensembl Mouse
Match: Traf2 (TNF receptor-associated factor 2 [Source:MGI Symbol;Acc:MGI:101835])

HSP 1 Score: 86.6557 bits (213), Expect = 5.389e-18
Identity = 44/92 (47.83%), Postives = 60/92 (65.22%), Query Frame = -3

Query:   75 ELPVTPY----IWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAIL 338
            EL V+ Y    IWKI+ FT+K Q+A      +I S  FY+ R GYKM L+VYL+G+G  +GTH+S+FF + KG  D L  WP++ K TL +L
Sbjct:  351 ELEVSTYDGVFIWKISDFTRKRQEAVAGRTPAIFSPAFYTSRYGYKMCLRVYLNGDGTGRGTHLSLFFVVMKGPNDALLQWPFNQKVTLMLL 442

[Close]

BLAST of SMED30000484 vs. Ensembl Mouse
Match: Traf2 (TNF receptor-associated factor 2 [Source:MGI Symbol;Acc:MGI:101835])

HSP 1 Score: 86.6557 bits (213), Expect = 5.686e-18
Identity = 44/92 (47.83%), Postives = 60/92 (65.22%), Query Frame = -3

Query:   75 ELPVTPY----IWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAIL 338
            EL V+ Y    IWKI+ FT+K Q+A      +I S  FY+ R GYKM L+VYL+G+G  +GTH+S+FF + KG  D L  WP++ K TL +L
Sbjct:  344 ELEVSTYDGVFIWKISDFTRKRQEAVAGRTPAIFSPAFYTSRYGYKMCLRVYLNGDGTGRGTHLSLFFVVMKGPNDALLQWPFNQKVTLMLL 435

[Close]

BLAST of SMED30000484 vs. Ensembl Mouse
Match: Traf5 (TNF receptor-associated factor 5 [Source:MGI Symbol;Acc:MGI:107548])

HSP 1 Score: 80.4925 bits (197), Expect = 7.035e-17
Identity = 33/81 (40.74%), Postives = 55/81 (67.90%), Query Frame = -3

Query:   75 IWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAIL 317
            IWK+T +  K ++A +   +S+ S PFY+ R GY++  + YL+G+G  KGTH+S++F + +G+FD L  WP+  + TL +L
Sbjct:   99 IWKVTDYRVKKREAVEGHTVSVFSQPFYTSRCGYRLCARAYLNGDGSGKGTHLSLYFVVMRGEFDSLLQWPFRQRVTLMLL 179

[Close]

Query:   75 IWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAIL 317
            IWK+T +  K ++A +   +S+ S PFY+ R GY++  + YL+G+G  KGTH+S++F + +G+FD L  WP+  + TL +L
Sbjct:   99 IWKVTDYRVKKREAVEGHTVSVFSQPFYTSRCGYRLCARAYLNGDGSGKGTHLSLYFVVMRGEFDSLLQWPFRQRVTLMLL 179

[Close]

BLAST of SMED30000484 vs. Ensembl Mouse
Match: Traf5 (TNF receptor-associated factor 5 [Source:MGI Symbol;Acc:MGI:107548])

HSP 1 Score: 82.4185 bits (202), Expect = 1.635e-16
Identity = 33/81 (40.74%), Postives = 55/81 (67.90%), Query Frame = -3

Query:   75 IWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAIL 317
            IWK+T +  K ++A +   +S+ S PFY+ R GY++  + YL+G+G  KGTH+S++F + +G+FD L  WP+  + TL +L
Sbjct:  401 IWKVTDYRVKKREAVEGHTVSVFSQPFYTSRCGYRLCARAYLNGDGSGKGTHLSLYFVVMRGEFDSLLQWPFRQRVTLMLL 481

[Close]

BLAST of SMED30000484 vs. UniProt/SwissProt
Match: sp|P39429|TRAF2_MOUSE (TNF receptor-associated factor 2 OS=Mus musculus OX=10090 GN=Traf2 PE=1 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 3.977e-17
Identity = 44/92 (47.83%), Postives = 60/92 (65.22%), Query Frame = -3

Query:   75 ELPVTPY----IWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAIL 338
            EL V+ Y    IWKI+ FT+K Q+A      +I S  FY+ R GYKM L+VYL+G+G  +GTH+S+FF + KG  D L  WP++ K TL +L
Sbjct:  344 ELEVSTYDGVFIWKISDFTRKRQEAVAGRTPAIFSPAFYTSRYGYKMCLRVYLNGDGTGRGTHLSLFFVVMKGPNDALLQWPFNQKVTLMLL 435

[Close]

BLAST of SMED30000484 vs. UniProt/SwissProt
Match: sp|Q12933|TRAF2_HUMAN (TNF receptor-associated factor 2 OS=Homo sapiens OX=9606 GN=TRAF2 PE=1 SV=2)

HSP 1 Score: 83.5741 bits (205), Expect = 3.539e-16
Identity = 39/86 (45.35%), Postives = 57/86 (66.28%), Query Frame = -3

Query:   63 YIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCAND 320
            +IWKI+ F +K Q+A      +I S  FY+ R GYKM L++YL+G+G  +GTH+S+FF + KG  D L  WP++ K TL +L  N+
Sbjct:  354 FIWKISDFARKRQEAVAGRIPAIFSPAFYTSRYGYKMCLRIYLNGDGTGRGTHLSLFFVVMKGPNDALLRWPFNQKVTLMLLDQNN 439

[Close]

BLAST of SMED30000484 vs. UniProt/SwissProt
Match: sp|P70191|TRAF5_MOUSE (TNF receptor-associated factor 5 OS=Mus musculus OX=10090 GN=Traf5 PE=1 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 1.410e-15
Identity = 33/81 (40.74%), Postives = 55/81 (67.90%), Query Frame = -3

Query:   75 IWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAIL 317
            IWK+T +  K ++A +   +S+ S PFY+ R GY++  + YL+G+G  KGTH+S++F + +G+FD L  WP+  + TL +L
Sbjct:  407 IWKVTDYRVKKREAVEGHTVSVFSQPFYTSRCGYRLCARAYLNGDGSGKGTHLSLYFVVMRGEFDSLLQWPFRQRVTLMLL 487

[Close]

BLAST of SMED30000484 vs. UniProt/SwissProt
Match: sp|O00463|TRAF5_HUMAN (TNF receptor-associated factor 5 OS=Homo sapiens OX=9606 GN=TRAF5 PE=1 SV=2)

HSP 1 Score: 82.0333 bits (201), Expect = 1.542e-15
Identity = 39/98 (39.80%), Postives = 61/98 (62.24%), Query Frame = -3

Query:   39 IWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCANDISA-----ETFKP 317
            IWK+T +  K ++A D   +SI S  FY+ R GY++  + YL+G+G  +G+H+S++F + +G+FD L  WP+  + TL +L   D S      ETFKP
Sbjct:  407 IWKVTDYKMKKREAVDGHTVSIFSQSFYTSRCGYRLCARAYLNGDGSGRGSHLSLYFVVMRGEFDSLLQWPFRQRVTLMLL---DQSGKKNIMETFKP 501

[Close]

BLAST of SMED30000484 vs. UniProt/SwissProt
Match: sp|P39428|TRAF1_MOUSE (TNF receptor-associated factor 1 OS=Mus musculus OX=10090 GN=Traf1 PE=1 SV=2)

HSP 1 Score: 78.5666 bits (192), Expect = 1.040e-14
Identity = 35/96 (36.46%), Postives = 61/96 (63.54%), Query Frame = -3

Query:   39 YIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCAN--DISAETFKP 320
            ++WKIT  TK+  ++     +S+ S  FY+ + GYK+ L++YL+G+G  K TH+S+F  + +G++D L  WP+ NK T  +L  N  + + + F+P
Sbjct:  262 FLWKITNVTKRCHESVCGRTVSLFSPAFYTAKYGYKLCLRLYLNGDGSGKKTHLSLFIVIMRGEYDALLPWPFRNKVTFMLLDQNNREHAIDAFRP 357

[Close]

BLAST of SMED30000484 vs. TrEMBL
Match: D5JG59 (TNF receptor-associated factor-2-like protein A OS=Dugesia japonica OX=6161 GN=TRAF-2A PE=2 SV=1)

HSP 1 Score: 118.627 bits (296), Expect = 2.611e-26
Identity = 92/273 (33.70%), Postives = 146/273 (53.48%), Query Frame = -3

Query:   39 EMR-IKCEHCNKTKTWIKIQNEHKNIKDEKVCKKFKRKCPNQYG-LEKVELKEHWGKYNKRKVDCPLRKWDAK-KKTVKEEMPQHLQMKIEEHA*I*MTALRENKLK-IDDIVIEVESHKILMTEIFDTREAFELARQRTTDTXXXXXXXXXXXXXXXXXXXXXIPTAGEL-PVTPYIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCANDIS---AETFKP 833
            EMR + C++C  T     +   H N   +++C K+   C N      KV LK+H      +K++CP  ++D K  K  KE++ +HL  +   H     T+   NK+K ++  +I++ES   L  +I  T+E FE+ ++ +   ++I + I E  K+     ++ I     +     +IWK T F  +   A    ++S+TS  FY+   GYKM L+VY  G+G  KGTH+SVFFA+ KG+FD+L  WP+  K TL+IL     S   ++TFKP
Sbjct:   36 EMRMVNCDYCGITMKSKDLNIIHLNPDRDELCSKYVGICSNNCNETRKVNLKDHLKICPNKKLECPFHQFDCKIDKMTKEKLMKHLDEEYFNH-----TSSLINKVKDLESKIIDLESE--LHNQILITKELFEMNKKSSESRLKIEKTIEELKKKAPATSIQTIDNKNVIFRNASFIWKFTDFEVRRVDAESGGRVSVTSDAFYTSEFGYKMCLRVYPAGDGVGKGTHLSVFFAIMKGEFDDLLSWPFKQKVTLSILDQESGSKPHSDTFKP 301

[Close]

BLAST of SMED30000484 vs. TrEMBL
Match: D5JG60 (TNF receptor-associated factor-2-like protein B OS=Dugesia japonica OX=6161 GN=TRAF-2B PE=2 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 1.693e-21
Identity = 84/277 (30.32%), Postives = 131/277 (47.29%), Query Frame = -3

Query:   39 MR-IKCEHCNKTKTWIKIQNEHKNIKDEKVCKKFKRKCPNQYGLEK-VELKEHWGKYNKRKVDCPLRKWDAKKKTVKEEMPQHLQMKIEEHA*I*MTALRENKLKIDDIVIEVESHKIL-------MTEIFDTREAFELARQRTTDTXXXXXXXXXXXXXXXXXXXXXIPTAGELPVTPYIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILC----ANDISAETFKP 830
            MR + C HC +   +  +   H N+  EK+C K   KCP +    K V L +H    +K+ V C   +    +   KE++ +H   +  +H+ + M  +   K  I ++ ++     IL        +EI D  ++ E   + T+ +   +    E                       +IWKI  F++   +A    +ISITS  FY+   GYKM +K+Y  G+G  KGTH+SVFF L +G+FD+L  WP+ NK TL IL      ND  ++TFKP
Sbjct:  143 MRLVSCTHCGEEMEYSNLTELHLNVHTEKLCAKLVGKCPYKCDNGKEVNLLDHRKSCSKKPVTCLFSEIGCNRVMTKEDLIKHNNEESLQHSRLLMKEIITMKNDISELKMKTLEFNILKIEFSKFQSEILDKIKSLEKIPRHTSPSTSSVRSSEEKTVYRNGV---------------FIWKIERFSELKSEAERGNRISITSDYFYTKEFGYKMSMKIYPAGDGVGKGTHLSVFFTLMRGEFDDLLQWPFKNKVTLTILDQKNKTND-HSDTFKP 403

[Close]

BLAST of SMED30000484 vs. TrEMBL
Match: F1AGB4 (TNF receptor-associated factor (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 6.071e-20
Identity = 41/90 (45.56%), Postives = 57/90 (63.33%), Query Frame = -3

Query:   63 PVTPYIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCAND 332
            P  P+IWKIT F +K Q+A+D TQ  + S P YSW NGYK+ +++Y +G+G   GTHISVF  + +G +DEL  WP+     L +    D
Sbjct:   12 PTAPFIWKITEFAEKLQRAKDGTQRILDSPPIYSWENGYKLGIRLYPNGDGNATGTHISVFIRVGRGDYDELLTWPFKQNMILTMKGQED 101

[Close]

BLAST of SMED30000484 vs. TrEMBL
Match: C1LGV7 (TNF receptor-associated factor 3 OS=Schistosoma japonicum OX=6182 GN=TRAF3 PE=2 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 1.161e-16
Identity = 75/282 (26.60%), Postives = 128/282 (45.39%), Query Frame = -3

Query:    9 CYEMRIKCEHCNKTKTWIKIQNEHKNIKDEKVCKKFKRKCPNQYGLEKVE---LKEHW-GKYNKRKVDCPLRKWDAKKKTVKEEMPQHLQMKIEEHA*I*MTALRENKLKIDDIVIEVESHKILMTEIFDTREAFELARQRTTDTXXXXXXXXXXXXXXXXXXXXXIPTAGELPVTPYIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCAN-DISAETFKPACVRIYPSFS 839
            C +  I+C+ C        ++ E K+I     C+++   CPN+  +  +    + EH   + +++ + CP      + +  K +M QHL++ + +H       L    L +  + + +E+     TEI   R + E   +R T                         T  +    P++WKI  +++K   AR   Q ++ S PFY+ R GY+M L V L GNGE KG  +SV+  L +G  D L  WP+S++ T  ++  N +I+A   KP    I PS +
Sbjct:  205 CPKRNIQCKFCG---AKFSVEKEAKHIS---YCREYPLPCPNKCEMTSIPRSLIPEHLKNECSRQNLRCPFNIHGCEYRGRKYKMDQHLELSLIKH-------LNLLNLSVQQMSLLIEAQTKTYTEI---RNSLEYQMKRIT-------------------------TLEQCFGPPFLWKIDSYSEKLNNARTGKQNALFSTPFYTHRFGYRMALSVSLYGNGEAKGKFMSVYACLFRGDHDSLLQWPFSHQLTFTLIDQNPEINAR--KPIVYTIKPSLT 443

[Close]

BLAST of SMED30000484 vs. TrEMBL
Match: A0A4Z2D8H8 (TNF receptor-associated factor 4 OS=Schistosoma japonicum OX=6182 GN=EWB00_003737 PE=4 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 1.228e-16
Identity = 75/282 (26.60%), Postives = 128/282 (45.39%), Query Frame = -3

Query:    9 CYEMRIKCEHCNKTKTWIKIQNEHKNIKDEKVCKKFKRKCPNQYGLEKVE---LKEHW-GKYNKRKVDCPLRKWDAKKKTVKEEMPQHLQMKIEEHA*I*MTALRENKLKIDDIVIEVESHKILMTEIFDTREAFELARQRTTDTXXXXXXXXXXXXXXXXXXXXXIPTAGELPVTPYIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCAN-DISAETFKPACVRIYPSFS 839
            C +  I+C+ C        ++ E K+I     C+++   CPN+  +  +    + EH   + +++ + CP      + +  K +M QHL++ + +H       L    L +  + + +E+     TEI   R + E   +R T                         T  +    P++WKI  +++K   AR   Q ++ S PFY+ R GY+M L V L GNGE KG  +SV+  L +G  D L  WP+S++ T  ++  N +I+A   KP    I PS +
Sbjct:  205 CPKRNIQCKFCG---AKFSVEKEAKHIS---YCREYPLPCPNKCEMTSIPRSLIPEHLKNECSRQNLRCPFNIHGCEYRGRKYKMDQHLELSLIKH-------LNLLNLSVQQMSLLIEAQTKTYTEI---RNSLEYQMKRIT-------------------------TLEQCFGPPFLWKIDSYSEKLNNARTGKQNALFSTPFYTHRFGYRMALSVSLYGNGEAKGKFMSVYACLFRGDHDSLLQWPFSHQLTFTLIDQNPEINAR--KPIVYTIKPSLT 443

[Close]

BLAST of SMED30000484 vs. Ensembl Cavefish
Match: traf2b (TNF receptor associated factor 2 [Source:NCBI gene;Acc:103028261])

HSP 1 Score: 83.9593 bits (206), Expect = 3.184e-17
Identity = 38/96 (39.58%), Postives = 63/96 (65.62%), Query Frame = -3

Query:   39 YIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCANDIS--AETFKP 320
            ++WKI+ F+++ Q A      ++ S  FYS + GYKM L++YL+G+G  +G+H+S+FF + +GK+D L  WP+S K TL +L  N+     + F+P
Sbjct:  384 FVWKISDFSRRRQDALAGRAPAMFSPAFYSSKYGYKMCLRLYLNGDGTGRGSHLSLFFVVMRGKYDALLKWPFSQKVTLMLLDQNNREHIIDAFRP 479

[Close]

BLAST of SMED30000484 vs. Ensembl Cavefish
Match: traf4a (TNF receptor associated factor 4 [Source:NCBI gene;Acc:103024022])

HSP 1 Score: 83.5741 bits (205), Expect = 4.390e-17
Identity = 63/274 (22.99%), Postives = 111/274 (40.51%), Query Frame = -3

Query:   39 CEHCNKTKTWIKIQNEHKNIKDEKVCKKFKRKCPNQYGLEKV-------ELKEHWGKYNKRKVDCPLRKWDAKKKTVKEEMPQHLQMKIEEHA*I*MTALRENKLKIDDIVIEVESHKILMTEIFDTREAFELARQRTTDTXXXXXXXXXXXXXXXXXXXXXIPTAGELPVTPYIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCANDIS-------AETFKP 818
            C++C K   +  IQN   +      C +F  +CPNQ G   +        +KE  G      V CP +    K +  K  + +HL+   + H  +  + +   + +I ++  E+E   +    +                                                  IWK+  +++K Q+A+        S PFY+ R GYK+++  +L+GNG  +G+H+S++  +  G++D L  WP++ K T +IL  +D S        ETF P
Sbjct:  195 CKYCGKEFVYDTIQNHQYH------CPRFPVQCPNQCGTPNIAREDLATHVKESCGS---ALVLCPFKDAGCKHRCPKLAIGRHLEETTKSHLTMMCSLVGRQRQEIIELRREMEELSVSHDGVL-------------------------------------------------IWKLNDYSRKLQEAKLHNNHEFFSPPFYTHRYGYKLQVSAFLNGNGSGEGSHLSIYIRVLPGEYDNLLEWPFAYKVTFSILDQSDPSLSKPQHITETFNP 410

[Close]

BLAST of SMED30000484 vs. Ensembl Cavefish
Match: ENSAMXT00000035169.1 (pep primary_assembly:Astyanax_mexicanus-2.0:9:3354744:3362219:1 gene:ENSAMXG00000029095.1 transcript:ENSAMXT00000035169.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 77.7962 bits (190), Expect = 9.428e-17
Identity = 38/96 (39.58%), Postives = 58/96 (60.42%), Query Frame = -3

Query:   39 IWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAIL---CANDISAETFKP 317
            IWKI  + ++  +A     +S+ S PFY+   GYKM  +VYL+G+G  KGTH+S+FF + +G++D L  WP+  K TL ++    A     + FKP
Sbjct:    7 IWKIRDYKRRKAEAVASKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVVMRGEYDALLPWPFKQKVTLMLMDQGPARKHLGDAFKP 102

[Close]

BLAST of SMED30000484 vs. Ensembl Cavefish
Match: traf2a (TNF receptor-associated factor 2-like [Source:NCBI gene;Acc:103036375])

HSP 1 Score: 81.2629 bits (199), Expect = 1.862e-16
Identity = 40/104 (38.46%), Postives = 63/104 (60.58%), Query Frame = -3

Query:   42 YIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCAN-----------DISAETFK 320
            ++WKI+ F+KK Q A      ++ S  FY+ + GYKM L++YL+G+G  +GTH+S+FF + +G  D L  WP++ K TL +L  N           DIS+ +F+
Sbjct:  312 FVWKISDFSKKRQDAVAGRAPAMFSPAFYTSKYGYKMCLRIYLNGDGTGRGTHLSLFFVVMRGHSDALLKWPFNQKVTLMLLDQNNREHIIDAFRPDISSSSFQ 415

[Close]

BLAST of SMED30000484 vs. Ensembl Cavefish
Match: traf2a (TNF receptor-associated factor 2-like [Source:NCBI gene;Acc:103036375])

HSP 1 Score: 81.6481 bits (200), Expect = 2.144e-16
Identity = 40/104 (38.46%), Postives = 63/104 (60.58%), Query Frame = -3

Query:   42 YIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCAN-----------DISAETFK 320
            ++WKI+ F+KK Q A      ++ S  FY+ + GYKM L++YL+G+G  +GTH+S+FF + +G  D L  WP++ K TL +L  N           DIS+ +F+
Sbjct:  425 FVWKISDFSKKRQDAVAGRAPAMFSPAFYTSKYGYKMCLRIYLNGDGTGRGTHLSLFFVVMRGHSDALLKWPFNQKVTLMLLDQNNREHIIDAFRPDISSSSFQ 528

[Close]

BLAST of SMED30000484 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002130.1 (pep scaffold:Pmarinus_7.0:GL484662:8457:12718:-1 gene:ENSPMAG00000001936.1 transcript:ENSPMAT00000002130.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 80.1073 bits (196), Expect = 7.991e-17
Identity = 36/96 (37.50%), Postives = 60/96 (62.50%), Query Frame = -3

Query:   39 IWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAIL---CANDISAETFKP 317
            +WKI  + ++ Q+A     +S+ S PFY+   GYKM  + YL+G+G  +GTH+S+FF + +G++D L  WP+  + TL++L    A    ++TF P
Sbjct:  271 VWKIRDYARRKQEAASGRTLSLYSQPFYTSPFGYKMCARAYLNGDGVGRGTHLSLFFVVMRGEYDALLEWPFRRRVTLSLLDQGAARRHLSDTFTP 366

[Close]

BLAST of SMED30000484 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000074.1 (pep scaffold:Pmarinus_7.0:GL482142:165593:173010:-1 gene:ENSPMAG00000000066.1 transcript:ENSPMAT00000000074.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 79.7221 bits (195), Expect = 1.296e-16
Identity = 36/96 (37.50%), Postives = 59/96 (61.46%), Query Frame = -3

Query:   39 IWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAIL---CANDISAETFKP 317
            +WKI  + ++ Q A     +S+ S PFY+   GY+M  + YL+G+G  +GTH+S+FF + +G++D L  WP+  + TL +L    A    ++TFKP
Sbjct:  328 VWKIRDYARRKQDAVSGRTLSLYSQPFYTSPFGYRMCARAYLNGDGVGRGTHLSLFFVVMRGEYDALLPWPFRQRVTLTLLDQGAARRQLSDTFKP 423

[Close]

BLAST of SMED30000484 vs. Ensembl Nematostella
Match: EDO49464 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RG36])

HSP 1 Score: 84.7297 bits (208), Expect = 5.978e-18
Identity = 40/96 (41.67%), Postives = 59/96 (61.46%), Query Frame = -3

Query:   39 YIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCANDIS--AETFKP 320
            YIWKI  +T+++Q+       SI S PFY  R GYK   +VY +G+G  KG+H+S+FF L +G+FD L  WP+  K T  +L  + +    +TF+P
Sbjct:  380 YIWKIDEYTRRYQEGVSGKTPSIYSPPFYVGRYGYKACARVYPNGDGMGKGSHLSLFFVLMRGEFDALLPWPFRQKVTFKLLDQDRVHDIGDTFRP 475

[Close]

BLAST of SMED30000484 vs. Ensembl Nematostella
Match: EDO36181 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJA2])

HSP 1 Score: 75.485 bits (184), Expect = 9.548e-17
Identity = 36/95 (37.89%), Postives = 60/95 (63.16%), Query Frame = -3

Query:   39 YIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCANDISAETF-KP 320
            ++W+I  +  +++K +     +I+S  FY+ R GYKM+LK +L+GNG+ KGTH+S+F  + KG++D L  WP+  K TL ++   D     + KP
Sbjct:   17 FVWRIRNYNDQYEKKQ-----TISSRLFYTSRYGYKMRLKSHLNGNGDGKGTHLSLFVQITKGEYDALLEWPFKRKITLYLIDQIDQQRHMYDKP 106

[Close]

BLAST of SMED30000484 vs. Ensembl Nematostella
Match: EDO29145 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T4G8])

HSP 1 Score: 68.5514 bits (166), Expect = 1.111e-14
Identity = 30/83 (36.14%), Postives = 53/83 (63.86%), Query Frame = -3

Query:   75 YIWKITGFTKKWQKA-RDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAIL 320
            ++WKI+ +  ++ +A     +++I S PFY+ + GYK++LK YL+G    KGTH+S++  + KG +D L  WP+  K T  ++
Sbjct:    4 FLWKISNYKHQYDQAVASPEELAIFSPPFYTSQYGYKLRLKAYLNGRDRGKGTHLSLYIIIMKGDYDALLEWPFKQKITFYLI 86

[Close]

BLAST of SMED30000484 vs. Ensembl Nematostella
Match: EDO32849 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7STV3])

HSP 1 Score: 74.3294 bits (181), Expect = 1.298e-14
Identity = 56/224 (25.00%), Postives = 103/224 (45.98%), Query Frame = -3

Query:   75 CPNQYG----LEKVELKEHWGKYNKR-KVDCPLRKWDAKKKTVKEEMPQHLQMKIEEHA*I*MTALRENKLKIDDIVIEVESHKILMTEIFDTREAFELARQRTTDTXXXXXXXXXXXXXXXXXXXXXIPTAGELPVTPY---IWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAIL 722
            CPN+      + + +L EH  K   R K+ C       + +  +E+M +H++ +I  H  +    + E K K+D+   + E H+ +M ++ D+R                                      G+L    +   IW+I  +T+K ++A+     ++ S  F + ++GY++   V L+G+G+ KGTH+SVF ++ KG FD L  WP+  + T  +L
Sbjct:  204 CPNKCDKTLTIPRGQLDEHIEKECPRAKLMCEFESLGCQHRCSREKMAKHMKSEIIGHVKMIFGVVLEQKEKLDNHAQKFEEHETIMKQV-DSR-------------------------------------VGDLEKIAHSQIIWRIEDYTRKLKEAKAGNAETLFSPTFTTSKHGYRLCASVCLNGDGKGKGTHMSVFVSVLKGAFDALLKWPFHYRVTFFLL 389

[Close]

BLAST of SMED30000484 vs. Ensembl Nematostella
Match: EDO48394 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJK8])

HSP 1 Score: 73.559 bits (179), Expect = 2.707e-14
Identity = 39/121 (32.23%), Postives = 66/121 (54.55%), Query Frame = -3

Query:   15 YIWKITGFTKKWQKA----------------RDETQIS---ITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCANDISAETFKPACVRIYPS 320
            ++W+I  +  K++KA                 D+  ++   + SLPFY+ R GYKM+LK Y +G G+ KGTH+S+   + KG++D +  WP+  K TL+++      +E ++   V IYP 
Sbjct:  322 FVWRICNYKDKYKKAVAFATNQEVKGGNSDSDDDISMAGDIMVSLPFYTSRYGYKMRLKAYPNGYGDGKGTHLSLSIRIMKGEYDAILEWPFRRKITLSLI----NQSEQWRSETVEIYPD 438

[Close]

BLAST of SMED30000484 vs. Ensembl Medaka
Match: ENSORLT00000020861.2 (TNF receptor-associated factor 2 [Source:NCBI gene;Acc:101167626])

HSP 1 Score: 83.9593 bits (206), Expect = 4.069e-17
Identity = 42/104 (40.38%), Postives = 63/104 (60.58%), Query Frame = -3

Query:   42 YIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCAN-----------DISAETFK 320
            +IWKI+ FTKK Q A      ++ S  FY+ + GYKM L++YL+G+G  +GTH+S+FF + +G  D L  WP++ K TL +L  N           DIS+ +F+
Sbjct:  422 FIWKISDFTKKRQDAMAGRAPAMFSPAFYTSKYGYKMCLRIYLNGDGTGRGTHLSLFFVVMRGHSDALLKWPFNQKVTLMLLDQNNREHIIDAFRPDISSSSFQ 525

[Close]

BLAST of SMED30000484 vs. Ensembl Medaka
Match: traf4a (TNF receptor associated factor 4 [Source:NCBI gene;Acc:101161428])

HSP 1 Score: 83.5741 bits (205), Expect = 4.218e-17
Identity = 64/271 (23.62%), Postives = 111/271 (40.96%), Query Frame = -3

Query:   39 CEHCNKTKTWIKIQNEHKNIKDEKVCKKFKRKCPNQYG---LEKVELKEHW-GKYNKRKVDCPLRKWDAKKKTVKEEMPQHLQMKIEEHA*I*MTALRENKLKIDDIVIEVESHKILMTEIFDTREAFELARQRTTDTXXXXXXXXXXXXXXXXXXXXXIPTAGELPVTPYIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCANDIS-------AETFKP 818
            C++C K   +  IQN   +      C +F  +CPNQ G   + + +L  H         V CP +    K +  K  + +HL+   + H  +    +   + +I ++  E+E   +    +                                                  IWK+  +++K Q+A+  +     S PFY+ R GYK+++  +L+GNG  +G+H+SV+  +  G++D L  WP+S K T +IL  +D S        ETF P
Sbjct:  188 CKYCGKEFVFDTIQNHQFH------CPRFPVQCPNQCGTPNIAREDLANHVKDNCGSALVLCPFKDAGCKHRCPKLAIGRHLEDTTKSHLTMMCNLVSRQRQEILELRREMEELSVSHDGVL-------------------------------------------------IWKLNDYSRKLQEAKLRSNHEFFSPPFYTHRYGYKLQVSAFLNGNGSGEGSHLSVYIRVLPGEYDNLLEWPFSYKVTFSILDQSDPSLSKPQHITETFNP 403

[Close]

BLAST of SMED30000484 vs. Ensembl Medaka
Match: traf2b (TNF receptor associated factor 2 [Source:NCBI gene;Acc:101159764])

HSP 1 Score: 83.5741 bits (205), Expect = 4.643e-17
Identity = 39/96 (40.62%), Postives = 62/96 (64.58%), Query Frame = -3

Query:   39 YIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCANDIS--AETFKP 320
            ++WKIT F+ + Q+A      ++ S  FYS + GYKM L++YL+G+G  +GTH+S+FF + + K+D L  WP+S K TL +L  N+     + F+P
Sbjct:  376 FVWKITDFSHRRQEAVSGRAPAMFSPAFYSSKYGYKMCLRLYLNGDGTGRGTHLSLFFVVMRSKYDALLKWPFSQKVTLMLLDQNNREHIIDAFRP 471

[Close]

BLAST of SMED30000484 vs. Ensembl Medaka
Match: traf2b (TNF receptor associated factor 2 [Source:NCBI gene;Acc:101159764])

HSP 1 Score: 83.5741 bits (205), Expect = 4.740e-17
Identity = 39/96 (40.62%), Postives = 62/96 (64.58%), Query Frame = -3

Query:   39 YIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCANDIS--AETFKP 320
            ++WKIT F+ + Q+A      ++ S  FYS + GYKM L++YL+G+G  +GTH+S+FF + + K+D L  WP+S K TL +L  N+     + F+P
Sbjct:  369 FVWKITDFSHRRQEAVSGRAPAMFSPAFYSSKYGYKMCLRLYLNGDGTGRGTHLSLFFVVMRSKYDALLKWPFSQKVTLMLLDQNNREHIIDAFRP 464

[Close]

BLAST of SMED30000484 vs. Ensembl Medaka
Match: traf4a (TNF receptor associated factor 4 [Source:NCBI gene;Acc:101161428])

HSP 1 Score: 83.1889 bits (204), Expect = 5.336e-17
Identity = 64/271 (23.62%), Postives = 111/271 (40.96%), Query Frame = -3

Query:   39 CEHCNKTKTWIKIQNEHKNIKDEKVCKKFKRKCPNQYG---LEKVELKEHW-GKYNKRKVDCPLRKWDAKKKTVKEEMPQHLQMKIEEHA*I*MTALRENKLKIDDIVIEVESHKILMTEIFDTREAFELARQRTTDTXXXXXXXXXXXXXXXXXXXXXIPTAGELPVTPYIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCANDIS-------AETFKP 818
            C++C K   +  IQN   +      C +F  +CPNQ G   + + +L  H         V CP +    K +  K  + +HL+   + H  +    +   + +I ++  E+E   +    +                                                  IWK+  +++K Q+A+  +     S PFY+ R GYK+++  +L+GNG  +G+H+SV+  +  G++D L  WP+S K T +IL  +D S        ETF P
Sbjct:  195 CKYCGKEFVFDTIQNHQFH------CPRFPVQCPNQCGTPNIAREDLANHVKDNCGSALVLCPFKDAGCKHRCPKLAIGRHLEDTTKSHLTMMCNLVSRQRQEILELRREMEELSVSHDGVL-------------------------------------------------IWKLNDYSRKLQEAKLRSNHEFFSPPFYTHRYGYKLQVSAFLNGNGSGEGSHLSVYIRVLPGEYDNLLEWPFSYKVTFSILDQSDPSLSKPQHITETFNP 410

[Close]

BLAST of SMED30000484 vs. Planmine SMEST
Match: SMESG000022654.1 (SMESG000022654.1)

HSP 1 Score: 170.629 bits (431), Expect = 2.806e-46
Identity = 91/256 (35.55%), Postives = 146/256 (57.03%), Query Frame = -3

Query:   78 TCYEMRIKCEHCNKTKTWIKIQNEHKNIKDEKVCKKFKRKCPNQYGL-EKVELKEHWGKYNKRKVDCPLRKWDAKKKTVKEEMPQHLQMKIEEHA*I*MTALRENKLKIDDIVIEVESHKILMTEIFDTREAFELARQRTTDTXXXXXXXXXXXXXXXXXXXXXIPTAGELPVTPYIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAI 842
             C     KCE C     WI I+ EH+N K+E++C  ++  C N     +KV+LKEHW +   + V CPL  ++ + +    EM +H+     +H  I M  +     ++  +  E ++ K LM EI ++R+ FE ++  T   I  ME+ ++ +    + +    P + +LP TP+IWKITGF +K Q+A+D TQ ++ S P YSW NGYK+ +++Y +G+G   GTHISVF ++ KG++DEL  WP+     L +
Sbjct: 2080 VCISREEKCEFCGAIDEWINIEKEHRNPKEEQICDLYEGICANHCNQKDKVKLKEHWNECIGKPVKCPLELFNCQAQLPIAEMQKHMIENSIKHTEIMMKTIGMIAWEMKSLRTETDAQKALMREILESRKNFEKSQAITPKKIAEMEEKIQQIISSIKDLETTSPISRKLPTTPFIWKITGFAEKLQRAKDGTQRTLDSPPVYSWGNGYKLGIRLYPNGDGNATGTHISVFVSVGKGEYDELLTWPFKQNMILTM 2335

[Close]

BLAST of SMED30000484 vs. Planmine SMEST
Match: SMESG000023758.1 (SMESG000023758.1)

HSP 1 Score: 159.844 bits (403), Expect = 1.546e-42
Identity = 90/261 (34.48%), Postives = 143/261 (54.79%), Query Frame = -3

Query:   63 TCYEMRIKCEHCNKTKTWIKIQNEHKNIKDEKVCKKFKRKCPNQYGL-EKVELKEHWGKYNKRKVDCPLRKWDAKKKTVKEEMPQHLQMKIEEHA*I*MTALRENKLKIDDIVIEVESHKILMTEIFDTREAFELARQRTTDTXXXXXXXXXXXXXXXXXXXXXIPTAGELPVTPYIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCAND 842
             C +   KCE C     WIKI+ EH+N K+E++C  ++  C N     +KV+LKEHW + + + V CPL     + +    EM +H+     EH  I M  +     ++  +  E ++ K L+ EI ++R+ FE ++  T   +  +EK M+ M    +++      A + P  P+IWKIT F +K Q+A+D TQ  + S P YSW+NGYK+ +++Y +G+G   GTHISVF  + +G +DEL  WP+     L +    D
Sbjct: 3718 VCIKREEKCEFCGVIDEWIKIEMEHRNPKEEQICDLYEGTCANNCDKKDKVKLKEHWNECSGKPVKCPLELLKCQVRLPITEMQKHMMENSIEHTEIMMKTIGMLAWEVKSLRTENDAQKALIREILESRKNFERSQGNTLKKMAEIEKKMQQMTNSLKELETNSSIARKPPTAPFIWKITEFAEKLQRAKDGTQRILDSPPIYSWKNGYKLGIRLYPNGDGNATGTHISVFIRVGRGDYDELLTWPFKQNMILTMKGQED 3978

HSP 2 Score: 53.5286 bits (127), Expect = 2.743e-7
Identity = 21/45 (46.67%), Postives = 34/45 (75.56%), Query Frame = -3

Query:   78 MKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAI 212
            MKL+VY +G+G  KG ++SV+F LA+G++D    WP++ K T++I
Sbjct:    1 MKLRVYPNGDGVGKGKYLSVYFLLARGRYDGKLEWPFNRKVTISI 45

[Close]

BLAST of SMED30000484 vs. Planmine SMEST
Match: SMESG000010523.1 (SMESG000010523.1)

HSP 1 Score: 159.458 bits (402), Expect = 2.055e-42
Identity = 90/260 (34.62%), Postives = 144/260 (55.38%), Query Frame = -3

Query:   63 CYEMRIKCEHCNKTKTWIKIQNEHKNIKDEKVCKKFKRKCPNQYGL-EKVELKEHWGKYNKRKVDCPLRKWDAKKKTVKEEMPQHLQMKIEEHA*I*MTALRENKLKIDDIVIEVESHKILMTEIFDTREAFELARQRTTDTXXXXXXXXXXXXXXXXXXXXXIPTAGELPVTPYIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCAND 839
            C +   KCE C  T  WIKI+ EH+N K+E++C  ++  C N     +KV+LKEHW + + + V CPL  ++ + +    EM +H+     EH  I M  +     ++  +  E ++ K ++ EI ++R+ FE ++  T   +  MEK M+ M    +++        + P  P+IWKIT F +K Q+A+D TQ  + S P YSW NGYK+ +++Y +G+G   GTHISVF  + +G +DEL  WP+     L +    D
Sbjct: 3867 CIKREEKCEFCGVTDEWIKIEMEHRNPKEEQICDLYEGVCANNCDKKDKVKLKEHWNECSGKPVKCPLELFNCQTQLPMAEMQKHMMENSIEHTEIMMKTIGMLAWEVKSLRTENDAQKAIIREILESRKNFEKSQGDTLKKMVEMEKKMQQMTNNIKELETNNSITRKHPTAPFIWKITEFAEKLQRAKDGTQRILDSPPIYSWENGYKLGIRLYPNGDGNATGTHISVFIRVGRGDYDELLTWPFKQNMILTMKGQED 4126

HSP 2 Score: 86.2705 bits (212), Expect = 9.332e-18
Identity = 38/84 (45.24%), Postives = 58/84 (69.05%), Query Frame = -3

Query:   78 VTPYIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAI 329
            + PY WKI G+ +K + A +E  I ITS  FY+   GY+MKL+VY +G+G  KG ++SV+F LA+G++D    WP++ K T++I
Sbjct:  320 LAPYTWKIGGWAEKVKSAEEEKIIGITSHQFYTEIGGYRMKLRVYPNGDGVGKGKYLSVYFLLARGRYDGKLEWPFNRKVTISI 403

[Close]

BLAST of SMED30000484 vs. Planmine SMEST
Match: SMESG000010523.1 (SMESG000010523.1)

HSP 1 Score: 159.458 bits (402), Expect = 2.137e-42
Identity = 90/260 (34.62%), Postives = 144/260 (55.38%), Query Frame = -3

Query:   63 CYEMRIKCEHCNKTKTWIKIQNEHKNIKDEKVCKKFKRKCPNQYGL-EKVELKEHWGKYNKRKVDCPLRKWDAKKKTVKEEMPQHLQMKIEEHA*I*MTALRENKLKIDDIVIEVESHKILMTEIFDTREAFELARQRTTDTXXXXXXXXXXXXXXXXXXXXXIPTAGELPVTPYIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCAND 839
            C +   KCE C  T  WIKI+ EH+N K+E++C  ++  C N     +KV+LKEHW + + + V CPL  ++ + +    EM +H+     EH  I M  +     ++  +  E ++ K ++ EI ++R+ FE ++  T   +  MEK M+ M    +++        + P  P+IWKIT F +K Q+A+D TQ  + S P YSW NGYK+ +++Y +G+G   GTHISVF  + +G +DEL  WP+     L +    D
Sbjct: 3917 CIKREEKCEFCGVTDEWIKIEMEHRNPKEEQICDLYEGVCANNCDKKDKVKLKEHWNECSGKPVKCPLELFNCQTQLPMAEMQKHMMENSIEHTEIMMKTIGMLAWEVKSLRTENDAQKAIIREILESRKNFEKSQGDTLKKMVEMEKKMQQMTNNIKELETNNSITRKHPTAPFIWKITEFAEKLQRAKDGTQRILDSPPIYSWENGYKLGIRLYPNGDGNATGTHISVFIRVGRGDYDELLTWPFKQNMILTMKGQED 4176

HSP 2 Score: 86.2705 bits (212), Expect = 9.090e-18
Identity = 38/84 (45.24%), Postives = 58/84 (69.05%), Query Frame = -3

Query:   78 VTPYIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAI 329
            + PY WKI G+ +K + A +E  I ITS  FY+   GY+MKL+VY +G+G  KG ++SV+F LA+G++D    WP++ K T++I
Sbjct:  320 LAPYTWKIGGWAEKVKSAEEEKIIGITSHQFYTEIGGYRMKLRVYPNGDGVGKGKYLSVYFLLARGRYDGKLEWPFNRKVTISI 403

[Close]

BLAST of SMED30000484 vs. Planmine SMEST
Match: SMESG000010523.1 (SMESG000010523.1)

HSP 1 Score: 159.458 bits (402), Expect = 2.281e-42
Identity = 90/260 (34.62%), Postives = 144/260 (55.38%), Query Frame = -3

Query:   63 CYEMRIKCEHCNKTKTWIKIQNEHKNIKDEKVCKKFKRKCPNQYGL-EKVELKEHWGKYNKRKVDCPLRKWDAKKKTVKEEMPQHLQMKIEEHA*I*MTALRENKLKIDDIVIEVESHKILMTEIFDTREAFELARQRTTDTXXXXXXXXXXXXXXXXXXXXXIPTAGELPVTPYIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCAND 839
            C +   KCE C  T  WIKI+ EH+N K+E++C  ++  C N     +KV+LKEHW + + + V CPL  ++ + +    EM +H+     EH  I M  +     ++  +  E ++ K ++ EI ++R+ FE ++  T   +  MEK M+ M    +++        + P  P+IWKIT F +K Q+A+D TQ  + S P YSW NGYK+ +++Y +G+G   GTHISVF  + +G +DEL  WP+     L +    D
Sbjct: 3866 CIKREEKCEFCGVTDEWIKIEMEHRNPKEEQICDLYEGVCANNCDKKDKVKLKEHWNECSGKPVKCPLELFNCQTQLPMAEMQKHMMENSIEHTEIMMKTIGMLAWEVKSLRTENDAQKAIIREILESRKNFEKSQGDTLKKMVEMEKKMQQMTNNIKELETNNSITRKHPTAPFIWKITEFAEKLQRAKDGTQRILDSPPIYSWENGYKLGIRLYPNGDGNATGTHISVFIRVGRGDYDELLTWPFKQNMILTMKGQED 4125

HSP 2 Score: 85.8853 bits (211), Expect = 9.589e-18
Identity = 38/84 (45.24%), Postives = 58/84 (69.05%), Query Frame = -3

Query:   78 VTPYIWKITGFTKKWQKARDETQISITSLPFYSWRNGYKMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAI 329
            + PY WKI G+ +K + A +E  I ITS  FY+   GY+MKL+VY +G+G  KG ++SV+F LA+G++D    WP++ K T++I
Sbjct:  320 LAPYTWKIGGWAEKVKSAEEEKIIGITSHQFYTEIGGYRMKLRVYPNGDGVGKGKYLSVYFLLARGRYDGKLEWPFNRKVTISI 403

The following BLAST results are available for this feature:

BLAST of SMED30000484 vs. Ensembl Human
BLAST of SMED30000484 vs. Ensembl Celegans
BLAST of SMED30000484 vs. Ensembl Fly
BLAST of SMED30000484 vs. Ensembl Zebrafish
BLAST of SMED30000484 vs. Ensembl Xenopus
BLAST of SMED30000484 vs. Ensembl Mouse
BLAST of SMED30000484 vs. UniProt/SwissProt
BLAST of SMED30000484 vs. TrEMBL
BLAST of SMED30000484 vs. Ensembl Cavefish
BLAST of SMED30000484 vs. Ensembl Sea Lamprey
BLAST of SMED30000484 vs. Ensembl Yeast
BLAST of SMED30000484 vs. Ensembl Nematostella
BLAST of SMED30000484 vs. Ensembl Medaka
BLAST of SMED30000484 vs. Planmine SMEST

BLAST of SMED30000484 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
TRAF2	7.370e-17	45.35	TNF receptor associated factor 2 [Source:HGNC Symb...	[more]
TRAF5	3.210e-16	39.80	TNF receptor associated factor 5 [Source:HGNC Symb...	[more]
TRAF5	3.210e-16	39.80	TNF receptor associated factor 5 [Source:HGNC Symb...	[more]
TRAF5	3.210e-16	39.80	TNF receptor associated factor 5 [Source:HGNC Symb...	[more]
TRAF3	2.148e-15	43.21	TNF receptor associated factor 3 [Source:HGNC Symb...	[more]

BLAST of SMED30000484 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1


Match Name	E-value	Identity	Description
trf-1	2.901e-10	24.42	TNF Receptor Associated Factor (TRAF) homolog; TRF...	[more]

BLAST of SMED30000484 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2


Match Name	E-value	Identity	Description
Traf4	2.816e-13	35.80	gene:FBgn0026319 transcript:FBtr0334401	[more]
Traf4	3.760e-13	35.80	gene:FBgn0026319 transcript:FBtr0077429	[more]

BLAST of SMED30000484 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
traf4a	4.500e-18	23.53	tnf receptor-associated factor 4a [Source:ZFIN;Acc...	[more]
traf4a	4.500e-18	23.53	tnf receptor-associated factor 4a [Source:ZFIN;Acc...	[more]
traf2b	3.053e-17	41.67	Tnf receptor-associated factor 2b [Source:ZFIN;Acc...	[more]
traf2b	3.053e-17	41.67	Tnf receptor-associated factor 2b [Source:ZFIN;Acc...	[more]
traf2b	3.250e-17	41.67	Tnf receptor-associated factor 2b [Source:ZFIN;Acc...	[more]

BLAST of SMED30000484 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
hspb6	1.705e-16	39.58	heat shock protein family B (small) member 6 [Sour...	[more]
TRAF2	2.037e-16	41.67	TNF receptor associated factor 2 [Source:NCBI gene...	[more]
TRAF2	2.127e-16	41.67	TNF receptor associated factor 2 [Source:NCBI gene...	[more]
sytl2	1.706e-15	24.09	synaptotagmin like 2 [Source:Xenbase;Acc:XB-GENE-9...	[more]
ssh2	5.318e-15	41.98	slingshot protein phosphatase 2 [Source:Xenbase;Ac...	[more]

BLAST of SMED30000484 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Traf2	5.389e-18	47.83	TNF receptor-associated factor 2 [Source:MGI Symbo...	[more]
Traf2	5.686e-18	47.83	TNF receptor-associated factor 2 [Source:MGI Symbo...	[more]
Traf5	7.035e-17	40.74	TNF receptor-associated factor 5 [Source:MGI Symbo...	[more]
Traf5	7.035e-17	40.74	TNF receptor-associated factor 5 [Source:MGI Symbo...	[more]
Traf5	1.635e-16	40.74	TNF receptor-associated factor 5 [Source:MGI Symbo...	[more]

BLAST of SMED30000484 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|P39429\|TRAF2_MOUSE	3.977e-17	47.83	TNF receptor-associated factor 2 OS=Mus musculus O...	[more]
sp\|Q12933\|TRAF2_HUMAN	3.539e-16	45.35	TNF receptor-associated factor 2 OS=Homo sapiens O...	[more]
sp\|P70191\|TRAF5_MOUSE	1.410e-15	40.74	TNF receptor-associated factor 5 OS=Mus musculus O...	[more]
sp\|O00463\|TRAF5_HUMAN	1.542e-15	39.80	TNF receptor-associated factor 5 OS=Homo sapiens O...	[more]
sp\|P39428\|TRAF1_MOUSE	1.040e-14	36.46	TNF receptor-associated factor 1 OS=Mus musculus O...	[more]

BLAST of SMED30000484 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
D5JG59	2.611e-26	33.70	TNF receptor-associated factor-2-like protein A OS...	[more]
D5JG60	1.693e-21	30.32	TNF receptor-associated factor-2-like protein B OS...	[more]
F1AGB4	6.071e-20	45.56	TNF receptor-associated factor (Fragment) OS=Schmi...	[more]
C1LGV7	1.161e-16	26.60	TNF receptor-associated factor 3 OS=Schistosoma ja...	[more]
A0A4Z2D8H8	1.228e-16	26.60	TNF receptor-associated factor 4 OS=Schistosoma ja...	[more]

BLAST of SMED30000484 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
traf2b	3.184e-17	39.58	TNF receptor associated factor 2 [Source:NCBI gene...	[more]
traf4a	4.390e-17	22.99	TNF receptor associated factor 4 [Source:NCBI gene...	[more]
ENSAMXT00000035169.1	9.428e-17	39.58	pep primary_assembly:Astyanax_mexicanus-2.0:9:3354...	[more]
traf2a	1.862e-16	38.46	TNF receptor-associated factor 2-like [Source:NCBI...	[more]
traf2a	2.144e-16	38.46	TNF receptor-associated factor 2-like [Source:NCBI...	[more]

BLAST of SMED30000484 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2


Match Name	E-value	Identity	Description
ENSPMAT00000002130.1	7.991e-17	37.50	pep scaffold:Pmarinus_7.0:GL484662:8457:12718:-1 g...	[more]
ENSPMAT00000000074.1	1.296e-16	37.50	pep scaffold:Pmarinus_7.0:GL482142:165593:173010:-...	[more]

BLAST of SMED30000484 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30000484 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO49464	5.978e-18	41.67	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO36181	9.548e-17	37.89	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO29145	1.111e-14	36.14	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO32849	1.298e-14	25.00	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO48394	2.707e-14	32.23	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of SMED30000484 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSORLT00000020861.2	4.069e-17	40.38	TNF receptor-associated factor 2 [Source:NCBI gene...	[more]
traf4a	4.218e-17	23.62	TNF receptor associated factor 4 [Source:NCBI gene...	[more]
traf2b	4.643e-17	40.63	TNF receptor associated factor 2 [Source:NCBI gene...	[more]
traf2b	4.740e-17	40.63	TNF receptor associated factor 2 [Source:NCBI gene...	[more]
traf4a	5.336e-17	23.62	TNF receptor associated factor 4 [Source:NCBI gene...	[more]

BLAST of SMED30000484 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000022654.1	2.806e-46	35.55	SMESG000022654.1	[more]
SMESG000023758.1	1.546e-42	34.48	SMESG000023758.1	[more]
SMESG000010523.1	2.055e-42	34.62	SMESG000010523.1	[more]
SMESG000010523.1	2.137e-42	34.62	SMESG000010523.1	[more]
SMESG000010523.1	2.281e-42	34.62	SMESG000010523.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30000484 ID=SMED30000484|Name=SMED30000484|organism=Schmidtea mediterranea sexual|type=transcript|length=1177bp
AATTAAATGGAAAAGCTTGGGTAAATTCTGACGCAAGCAGGTTTGAATGT
TTCTGCTGATATATCGTTTGCACATAGTATTGCTAGAGTCGTTTTGTTGC
TATATGGCCATAGTTGTAACTCATCAAATTTTCCTTTAGCTAGTGCAAAA
AATACTGATATGTGTGTACCTTTTTCCTCACCGTTCCCGTCTAGATACAC
TTTCAGTTTCATTTTATATCCATTTCTCCATGAATAGAACGGTAGGCTCG
TTATTGATATTTGCGTTTCGTCTCTTGCTTTTTGCCATTTTTTGGTGAAG
CCCGTGATTTTCCATATGTATGGTGTGACTGGTAATTCTCCTGCGGTGGG
AATCAATTTCATCATTTTTTCGTATTATTCTTTCATCCCTTCCATTATTT
TCTCCATAATTTGTATGGTATCTGTCGTTCTTTGTCTCGCTAGTTCGAAT
GCTTCTCTGGTGTCAAAGATCTCCGTCATTAAGATTTTATGGGACTCCAC
TTCGATTACTATATCATCGATTTTTAGCTTATTTTCTCTTAAGGCTGTCA
TCTAGATTTATGCATGCTCTTCGATTTTCATTTGTAGGTGTTGTGGCATT
TCTTCTTTCACGGTTTTTTTCTTCGCATCCCATTTCCGCAAGGGGCAATC
TACTTTTCTTTTATTGTATTTTCCCCAATGCTCCTTAAGTTCTACTTTTT
CTAATCCGTATTGGTTCGGACACTTTCGTTTGAATTTTTTACAAACTTTT
TCATCTTTTATATTTTTATGTTCGTTCTGTATTTTTATCCACGTTTTGGT
TTTGTTGCAGTGTTCGCATTTTATTCTCATTTCATAACAAGTTTAAATAA
ATTTTATTTCAAGCAAAGCTGCTTCAAAGAGAAGATTATATCCCTTTTAA
CAGGGTAAGCTTCCGCATAAATTGCCAAAGTCATTATCTCCATTATTCGT
TTTCATCACTATTCATCTAGCCACAGTCCCAGGATACCATTTGCTTGCAG
AGCATCATTCCAGTCATACTTCAGGTTTTCATCTTATCATGGCATTTGCG
AATTCCAAGCATAAGTCAGCTGTTTCATCTCACCAGGGCATCTCATTCAG
TAGTCACAATTCATCATGGGATTATTCATTATTTCCAGTATTATTTGATA
AAATATATATTTTGTTATGTAAAAAAA

protein sequence of SMED30000484-orf-1

>SMED30000484-orf-1 ID=SMED30000484-orf-1|Name=SMED30000484-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=121bp
MMKLIPTAGELPVTPYIWKITGFTKKWQKARDETQISITSLPFYSWRNGY
KMKLKVYLDGNGEEKGTHISVFFALAKGKFDELQLWPYSNKTTLAILCAN
DISAETFKPACVRIYPSFSI*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0002109	X1 cell
PLANA:0002111	X2 cell

Vocabulary: **molecular function**
Term	Definition
GO:0005515	protein binding

Vocabulary: **INTERPRO**
Term	Definition
IPR008974	TRAF-like
IPR002083	MATH/TRAF_dom

Main menu

SMED30000484

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 2

Owlery

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