SMED30000449

Overview
NameSMED30000449
Smed IDSMED30000449
Length (bp)2039
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30000449 (SMED30000449) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30000449 (SMED30000449) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30000449 (SMED30000449) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30000449 (SMED30000449) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30000449

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
protonephridiaSMED30000449SMESG000020436.1 dd_Smed_v4_5646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30000449SMESG000020436.1 dd_Smed_v4_5646_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30000449SMESG000020436.1 Contig24624newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30000449SMESG000020436.1 Contig24624uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
epidermisSMED30000449SMESG000020436.1 dd_Smed_v4_5646_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30000449 vs. Ensembl Human
Match: EPB41 (erythrocyte membrane protein band 4.1 [Source:HGNC Symbol;Acc:HGNC:3377])

HSP 1 Score: 50.447 bits (119), Expect = 9.364e-6
Identity = 51/220 (23.18%), Postives = 95/220 (43.18%), Query Frame = 1 
Query:   79 ILLLDGKIL-CVYCKNISTRVLIKHLCKYLDLQRPETVGLLYKDSRGSLQWMNESEYILDHNIDIDHEFIQLIFKIRYVPHHLCYNESNIHQLFLLSVRLLKQNWFIAQIEIHSQLIALYILFKYGSQLNESEIDSAIHECNLSVD---APISNTNLLSMIVEAFNNYRSKLNSQADFLFKFLHLTLSFPIVNSKLYVVSDSKGQNLVLGINSVELTLFQ 726
            + LLD  +  CV  K+   + L+K +C++L+L   +  GL   D+  S  W++ ++ I      +   F    F +++ P        +I + +L     L+Q+  I    +      L +L  Y  Q    + D  +H  +   D   AP     L   ++E   +YRS   +QAD   +FL       +    L+   D +G +++LG+ S  L +++
Sbjct:    5 VSLLDDTVYECVVEKHAKGQDLLKRVCEHLNLLEEDYFGLAIWDNATSKTWLDSAKEIKKQVRGVPWNF---TFNVKFYPPDPAQLTEDITRYYL--CLQLRQD--IVAGRLPCSFATLALLGSYTIQSELGDYDPELHGVDYVSDFKLAPNQTKELEEKVMELHKSYRSMTPAQAD--LEFLENAKKLSMYGVDLHKAKDLEGVDIILGVCSSGLLVYK 215
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BLAST of SMED30000449 vs. Ensembl Zebrafish
Match: epb41b (erythrocyte membrane protein band 4.1b [Source:ZFIN;Acc:ZDB-GENE-030130-1])

HSP 1 Score: 52.373 bits (124), Expect = 2.254e-6
Identity = 49/199 (24.62%), Postives = 91/199 (45.73%), Query Frame = 1 
Query:  139 LIKHLCKYLDLQRPETVGLLYKDSRGSLQWMNESEYILDHNIDIDHEFIQLIFKIRYVPHHLCYNESNIHQLFLLSVRLLKQNWFIAQIEIHSQLIALYILFKYGSQLNESEIDSAIHECNLS---VDAPISNTNLLSMIVEAFNNYRSKLNSQADFLFKFLHLTLSFPIVNSKLYVVSDSKGQNLVLGINSVELTLFQ 726
            L   +C++L+L   +  GL+  DS  +  W++ ++ I      I     +  F I++ P        +I + FL     L+++  I ++   S ++AL  L  Y  Q    + D  +H+ N     V AP  +  L   ++E    YR    +QAD L  FL   +  P+    L+   D+ G++++LG+ S  L +++
Sbjct:   27 LFNKVCEHLNLLERDYFGLVMWDSPTNRVWLDCAKEIRKQ---IKSPVAEFFFSIKFYPPDPSILAEDITRYFL--CLHLRKDILIGRLPCPSDILAL--LGSYTVQSTLGDYDPNLHKNNYVRKIVLAPNQSKELEEKVMELHATYRFMSPAQADLL--FLENVMGLPMYGVDLHPAKDASGEDVMLGVCSEGLIVYE 216
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BLAST of SMED30000449 vs. Ensembl Zebrafish
Match: frmd4ba (FERM domain containing 4Ba [Source:ZFIN;Acc:ZDB-GENE-130412-1])

HSP 1 Score: 50.8322 bits (120), Expect = 5.486e-6
Identity = 56/282 (19.86%), Postives = 127/282 (45.04%), Query Frame = 1 
Query:   76 HILLLDGKILCVYCK-NISTRVLIKHLCKYLDLQRPETVGLLYKDSRGSLQWMNESEYILDHNIDIDHEFIQLIFKIRYVPHHLCY-NESNIHQLFLLSVRLLKQNWFIAQIEIHSQLI---ALYILFKY-GSQLNESEIDSAIHECNLSVDAPISNTNLLSM----IVEAFNNYRSKLNSQADFLFKFLHLTLSFPIVNSKLYVVSDSKGQNLVLGINSVELTLFQLEQFHCDLIKYKFSIVEKVMNRGIKIVIDLKYNKKRSLTPSRYLENEMA--TFYA 885
             +LLLD + L +  +  + +R L+  +  + +L+  +  G+ + D RG  +W+     +LDH+ +     ++L F++R+    + Y  ++   +LF  + ++   + +   IE+ S+++   A Y L +  G   ++    SA+ +      A +     L+     ++E +   +     QA  + ++L L  S P      Y V D +G    LGI+   +  + L+        + +  +E +  R  K  +++   ++R+ +   + ++  A  T+YA
Sbjct:    8 QVLLLDDRQLELLVQPKLLSRDLLDLVSSHFNLKEKDYFGISFTDERGQKKWLQLDRRVLDHDFNKKSGPLELKFQVRFYIESVAYLKDATTVELFFRNAKI---SVYNENIEVESEIVFKLAAYTLQEANGDYTSDESTRSALKKLPAFPTAVLKEHPSLTFCEDKVIERYKKLKGLSRGQA--IVRYLTLVESLPAYGVHYYKVKDKQGLPWWLGISYKGIGQYDLQDKLKPRKLFLWKQLENLYFREKKFAVEVNDPQRRTASRRTFGQSGQAIHTWYA 284
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BLAST of SMED30000449 vs. Ensembl Mouse
Match: Epb41 (erythrocyte membrane protein band 4.1 [Source:MGI Symbol;Acc:MGI:95401])

HSP 1 Score: 51.2174 bits (121), Expect = 3.831e-6
Identity = 52/220 (23.64%), Postives = 95/220 (43.18%), Query Frame = 1 
Query:   79 ILLLDGKIL-CVYCKNISTRVLIKHLCKYLDLQRPETVGLLYKDSRGSLQWMNESEYILDHNIDIDHEFIQLIFKIRYVPHHLCYNESNIHQLFLLSVRLLKQNWFIAQIEIHSQLIALYILFKYGSQLNESEIDSAIHECNLSVD---APISNTNLLSMIVEAFNNYRSKLNSQADFLFKFLHLTLSFPIVNSKLYVVSDSKGQNLVLGINSVELTLFQ 726
            + LLD  +  CV  K+   + L+K +C++L+L   +  GL   DS  S  W++ ++ I      +   F    F +++ P        +I + +L     L+Q+  I    +      L +L  Y  Q    + D  +H  +   D   AP     L   ++E   +YRS   +QAD   +FL       +    L+   D +G +++LG+ S  L +++
Sbjct:    5 VSLLDDTVYECVVEKHAKGQDLLKRVCEHLNLLEEDYFGLALWDSATSKTWLDSAKEIKKQVRGVPWNF---TFNVKFYPPDPAQLTEDITRYYL--CLQLRQD--IVAGRLPCSFATLALLGSYTIQSELGDYDPELHGMDYVSDFKLAPNQTKELEEKVMELHKSYRSMTPAQAD--LEFLENAKKLSMYGVDLHKAKDLEGVDIILGVCSSGLLVYK 215
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BLAST of SMED30000449 vs. Ensembl Mouse
Match: Epb41 (erythrocyte membrane protein band 4.1 [Source:MGI Symbol;Acc:MGI:95401])

HSP 1 Score: 50.8322 bits (120), Expect = 5.445e-6
Identity = 52/220 (23.64%), Postives = 95/220 (43.18%), Query Frame = 1 
Query:   79 ILLLDGKIL-CVYCKNISTRVLIKHLCKYLDLQRPETVGLLYKDSRGSLQWMNESEYILDHNIDIDHEFIQLIFKIRYVPHHLCYNESNIHQLFLLSVRLLKQNWFIAQIEIHSQLIALYILFKYGSQLNESEIDSAIHECNLSVD---APISNTNLLSMIVEAFNNYRSKLNSQADFLFKFLHLTLSFPIVNSKLYVVSDSKGQNLVLGINSVELTLFQ 726
            + LLD  +  CV  K+   + L+K +C++L+L   +  GL   DS  S  W++ ++ I      +   F    F +++ P        +I + +L     L+Q+  I    +      L +L  Y  Q    + D  +H  +   D   AP     L   ++E   +YRS   +QAD   +FL       +    L+   D +G +++LG+ S  L +++
Sbjct:   63 VSLLDDTVYECVVEKHAKGQDLLKRVCEHLNLLEEDYFGLALWDSATSKTWLDSAKEIKKQVRGVPWNF---TFNVKFYPPDPAQLTEDITRYYL--CLQLRQD--IVAGRLPCSFATLALLGSYTIQSELGDYDPELHGMDYVSDFKLAPNQTKELEEKVMELHKSYRSMTPAQAD--LEFLENAKKLSMYGVDLHKAKDLEGVDIILGVCSSGLLVYK 273
[Close]
BLAST of SMED30000449 vs. Ensembl Mouse
Match: Epb41 (erythrocyte membrane protein band 4.1 [Source:MGI Symbol;Acc:MGI:95401])

HSP 1 Score: 50.8322 bits (120), Expect = 5.887e-6
Identity = 51/220 (23.18%), Postives = 97/220 (44.09%), Query Frame = 1 
Query:   79 ILLLDGKIL-CVYCKNISTRVLIKHLCKYLDLQRPETVGLLYKDSRGSLQWMNESEYILDHNIDIDHEFIQLIFKIRYVPHHLCYNESNIHQLFLLSVRLLKQNWFIAQIEIHSQLIALYILFKYGSQLNESEIDSAIHECNLSVD---APISNTNLLSMIVEAFNNYRSKLNSQADFLFKFLHLTLSFPIVNSKLYVVSDSKGQNLVLGINSVELTLFQ 726
            + LLD  +  CV  K+   + L+K +C++L+L   +  GL   DS  S  W++ ++ I      +   F    F +++ P        +I + +L     L+Q+    ++      +AL   +   S+L   + D  +H  +   D   AP     L   ++E   +YRS   +QAD   +FL       +    L+   D +G +++LG+ S  L +++
Sbjct:   57 VSLLDDTVYECVVEKHAKGQDLLKRVCEHLNLLEEDYFGLALWDSATSKTWLDSAKEIKKQVRGVPWNF---TFNVKFYPPDPAQLTEDITRYYL--CLQLRQDIVAGRLPCSFATLALLGSYTIQSELG--DYDPELHGMDYVSDFKLAPNQTKELEEKVMELHKSYRSMTPAQAD--LEFLENAKKLSMYGVDLHKAKDLEGVDIILGVCSSGLLVYK 267
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BLAST of SMED30000449 vs. Planmine SMEST
Match: SMESG000020436.1 (SMESG000020436.1)

HSP 1 Score: 1142.49 bits (2954), Expect = 0.000e+0
Identity = 607/609 (99.67%), Postives = 608/609 (99.84%), Query Frame = 1 
Query:   58 MSDIVIHILLLDGKILCVYCKNISTRVLIKHLCKYLDLQRPETVGLLYKDSRGSLQWMNESEYILDHNIDIDHEFIQLIFKIRYVPHHLCYNESNIHQLFLLSVRLLKQNWFIAQIEIHSQLIALYILFKYGSQLNESEIDSAIHECNLSVDAPISNTNLLSMIVEAFNNYRSKLNSQADFLFKFLHLTLSFPIVNSKLYVVSDSKGQNLVLGINSVELTLFQLEQFHCDLIKYKFSIVEKVMNRGIKIVIDLKYNKKRSLTPSRYLENEMATFYALTAHQSRCIYAEIQSSRGQFQESSSVYKISDMMKNHLFLKSLNKWLYKYSSDINAEYSVDDIFICFEKDPCSDENEIEILFGEVDMYNSEIYQKLHQQKTVNSDIEIKPDFXXXXXXXXXXXXXXXXXXXXXXXXFVEEEKVTGIMPNEVLEYFPNGIPKFQRNVKTATLNYYQHRGCAASVRNRLMAADNVSRQGTIRSYDKFSSSRSVTDLSTMNTYXXXXXXXXXXXXXXXXXXXXXNVVHKSSGNLFFTDSKHIVTNPKNKIPKSKSQSSNIPSTLTMKPRIYNSISPSSPRSDVFLSSTPNHIPSTHSSPIMYPQKYKRIQNRSYERK 1884
            MSDIVIHILLLDGKILCVYCKNISTRVLIKHLCKYLDLQRPETVGLLYKDSRGSLQWMNESEYILDHNIDIDHEFIQLIFKIRYVPHHLCYNESNIHQLFLLSVRLLKQNWFIAQIEIHSQLIALYILFKYGSQLNESEIDSAIHECNLSVDAPISNTNLLSMIVEAFNNYRSKLNSQADFLFKFLHLTLSFPIVNSKLYVVSDSKGQNLVLGINSVELTLFQLEQFHCDLIKYKFSIVE VMNRGIKIVIDLKYNKKRSLTPSRYLENEMATFYALTAHQSRCIYAEIQSSRGQFQESSSVYKISDMMKNHLFLKSLNKWLYKYSSDINAEYSVDDIFICFEKDPCSDENEIEILFGEVDMYNSEIYQKLHQQKTVNSDIEIKPDFQQLVSEEKQLKELLQQKMIQLQELFVEEEKVTGIMPNEVLEYFPNGIPKFQRNVKTATLNYYQHRGCAASVRNRLMAADNVSRQGTIRSYDKFSSSRSVTDLST+NTYSPWKTKSDSSSSLRSFEDSASNVVHKSSGNLFFTDSKHIVTNPKNKIPKSKSQSSNIPSTLTMKPRIYNSISPSSPRSDVFLSSTPNHIPSTHSSPIMYPQKYKRIQNRSYERK
Sbjct:    1 MSDIVIHILLLDGKILCVYCKNISTRVLIKHLCKYLDLQRPETVGLLYKDSRGSLQWMNESEYILDHNIDIDHEFIQLIFKIRYVPHHLCYNESNIHQLFLLSVRLLKQNWFIAQIEIHSQLIALYILFKYGSQLNESEIDSAIHECNLSVDAPISNTNLLSMIVEAFNNYRSKLNSQADFLFKFLHLTLSFPIVNSKLYVVSDSKGQNLVLGINSVELTLFQLEQFHCDLIKYKFSIVENVMNRGIKIVIDLKYNKKRSLTPSRYLENEMATFYALTAHQSRCIYAEIQSSRGQFQESSSVYKISDMMKNHLFLKSLNKWLYKYSSDINAEYSVDDIFICFEKDPCSDENEIEILFGEVDMYNSEIYQKLHQQKTVNSDIEIKPDFQQLVSEEKQLKELLQQKMIQLQELFVEEEKVTGIMPNEVLEYFPNGIPKFQRNVKTATLNYYQHRGCAASVRNRLMAADNVSRQGTIRSYDKFSSSRSVTDLSTINTYSPWKTKSDSSSSLRSFEDSASNVVHKSSGNLFFTDSKHIVTNPKNKIPKSKSQSSNIPSTLTMKPRIYNSISPSSPRSDVFLSSTPNHIPSTHSSPIMYPQKYKRIQNRSYERK 609
The following BLAST results are available for this feature:
BLAST of SMED30000449 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
EPB419.364e-623.18erythrocyte membrane protein band 4.1 [Source:HGNC... [more]
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BLAST of SMED30000449 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000449 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000449 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
epb41b2.254e-624.62erythrocyte membrane protein band 4.1b [Source:ZFI... [more]
frmd4ba5.486e-619.86FERM domain containing 4Ba [Source:ZFIN;Acc:ZDB-GE... [more]
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BLAST of SMED30000449 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000449 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Epb413.831e-623.64erythrocyte membrane protein band 4.1 [Source:MGI ... [more]
Epb415.445e-623.64erythrocyte membrane protein band 4.1 [Source:MGI ... [more]
Epb415.887e-623.18erythrocyte membrane protein band 4.1 [Source:MGI ... [more]
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BLAST of SMED30000449 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000449 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000449 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000449 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000449 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000449 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000449 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000449 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000020436.10.000e+099.67SMESG000020436.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30000449 ID=SMED30000449|Name=SMED30000449|organism=Schmidtea mediterranea sexual|type=transcript|length=2039bp
TCAGACTATATTAAAATAGAAATTAAATTCTAAAATTTTTATTTTATTAT
TCCCTACATGTCTGACATTGTAATTCATATTTTATTGCTCGACGGAAAGA
TATTATGTGTCTATTGTAAAAACATATCAACCAGAGTTTTAATAAAACAT
TTATGTAAGTACTTAGATCTCCAGCGACCAGAAACTGTTGGATTATTATA
CAAAGACTCGAGAGGCTCATTACAATGGATGAATGAATCAGAATACATTT
TAGATCATAATATTGATATAGACCATGAATTTATTCAATTAATTTTTAAA
ATTAGGTATGTTCCTCATCATTTATGCTATAACGAATCCAATATTCACCA
ATTATTTCTGCTCTCCGTCAGATTACTTAAACAAAATTGGTTCATAGCAC
AGATAGAAATACATTCACAGTTGATAGCCCTGTATATATTATTCAAATAT
GGTAGCCAGTTGAATGAATCAGAAATTGATAGTGCAATTCATGAGTGTAA
TTTGTCAGTTGATGCTCCGATTTCCAACACAAACTTGTTGAGTATGATAG
TTGAAGCTTTCAATAATTACAGATCAAAATTGAATTCTCAGGCTGACTTC
TTATTCAAATTTCTTCATTTGACTTTGAGCTTTCCGATAGTAAATTCCAA
ATTATATGTTGTTTCAGATTCAAAAGGGCAGAATTTGGTTCTGGGAATAA
ATTCTGTAGAATTGACACTATTTCAATTAGAACAATTTCATTGTGATTTA
ATAAAGTATAAATTCAGTATTGTTGAAAAGGTTATGAATCGTGGAATTAA
AATTGTTATTGATCTCAAATATAATAAAAAAAGATCACTGACTCCGTCGA
GATATTTAGAGAATGAAATGGCAACATTTTATGCACTGACTGCCCACCAG
AGCCGTTGTATTTACGCCGAAATTCAATCTAGTCGTGGTCAGTTTCAAGA
ATCGTCATCAGTTTATAAAATAAGTGACATGATGAAAAATCATTTATTTC
TTAAAAGTCTTAACAAGTGGTTGTATAAATACTCGTCTGATATTAATGCA
GAGTACAGTGTAGATGATATATTCATATGCTTTGAAAAGGATCCGTGTTC
AGATGAGAATGAAATTGAAATTCTTTTCGGTGAAGTTGATATGTATAATA
GTGAGATCTACCAAAAATTGCATCAACAGAAAACCGTGAACTCAGACATT
GAAATAAAACCAGATTTCCAGCAATTAGTATCCGAAGAAAAACAATTAAA
GGAGTTGCTGCAGCAAAAAATGATTCAATTGCAAGAATTATTTGTTGAAG
AAGAGAAAGTTACCGGAATAATGCCAAATGAAGTTCTAGAATATTTTCCA
AATGGGATTCCAAAATTCCAAAGAAATGTCAAAACTGCAACGTTAAATTA
CTATCAACATCGAGGATGCGCTGCTAGTGTGAGAAACAGACTAATGGCAG
CGGATAATGTCAGTAGACAAGGGACCATTAGATCATATGACAAGTTCTCT
TCCAGCCGTTCGGTTACCGATCTCAGCACAATGAATACTTATTCTCCTTG
GAAAACTAAGTCAGACTCGAGTTCTTCGTTGAGGTCGTTTGAAGACAGTG
CATCGAATGTAGTTCACAAATCATCGGGCAATTTGTTTTTCACTGATTCA
AAGCACATTGTCACAAATCCAAAAAATAAAATACCAAAATCCAAATCTCA
AAGCAGCAATATCCCATCCACTCTTACAATGAAGCCTAGAATATATAATA
GTATTTCTCCGTCTTCTCCGAGGTCAGATGTGTTTCTGAGTAGCACCCCA
AACCATATTCCTTCGACTCATTCGAGTCCCATTATGTATCCACAAAAGTA
TAAAAGAATTCAGAATCGCAGTTATGAGAGGAAATGATGAATCAAGGCAA
ATCGCCAATGTAATGTACAATGCAGCACCGATTGAATTGAACATATCAAA
TCATCCAGATTTTTTAATTATTTTTTTTAATTAGTTTTGTTGTGTTTTTA
AATATTTTATTAAATATAGTTAAAATTTCTATACAACAA
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protein sequence of SMED30000449-orf-1

>SMED30000449-orf-1 ID=SMED30000449-orf-1|Name=SMED30000449-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=610bp
MSDIVIHILLLDGKILCVYCKNISTRVLIKHLCKYLDLQRPETVGLLYKD
SRGSLQWMNESEYILDHNIDIDHEFIQLIFKIRYVPHHLCYNESNIHQLF
LLSVRLLKQNWFIAQIEIHSQLIALYILFKYGSQLNESEIDSAIHECNLS
VDAPISNTNLLSMIVEAFNNYRSKLNSQADFLFKFLHLTLSFPIVNSKLY
VVSDSKGQNLVLGINSVELTLFQLEQFHCDLIKYKFSIVEKVMNRGIKIV
IDLKYNKKRSLTPSRYLENEMATFYALTAHQSRCIYAEIQSSRGQFQESS
SVYKISDMMKNHLFLKSLNKWLYKYSSDINAEYSVDDIFICFEKDPCSDE
NEIEILFGEVDMYNSEIYQKLHQQKTVNSDIEIKPDFQQLVSEEKQLKEL
LQQKMIQLQELFVEEEKVTGIMPNEVLEYFPNGIPKFQRNVKTATLNYYQ
HRGCAASVRNRLMAADNVSRQGTIRSYDKFSSSRSVTDLSTMNTYSPWKT
KSDSSSSLRSFEDSASNVVHKSSGNLFFTDSKHIVTNPKNKIPKSKSQSS
NIPSTLTMKPRIYNSISPSSPRSDVFLSSTPNHIPSTHSSPIMYPQKYKR
IQNRSYERK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000034epidermis
Vocabulary: INTERPRO
TermDefinition
IPR000299FERM_domain
IPR029071Ubiquitin-like_domsf
Vocabulary: cellular component
TermDefinition
GO:0005856cytoskeleton
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 390..411
NoneNo IPR availableGENE3DG3DSA:3.10.20.90coord: 2..86
e-value: 7.7E-6
score: 27.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 538..557
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 569..593
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 569..609
NoneNo IPR availableCDDcd01765FERM_F0_F1coord: 4..67
e-value: 2.6089E-6
score: 43.7303
IPR000299FERM domainPROSITEPS50057FERM_3coord: 4..300
score: 15.573
IPR029071Ubiquitin-like domain superfamilySUPERFAMILYSSF54236Ubiquitin-likecoord: 4..85