SMED30000404
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30000404 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 5
Alignments
SMED30000404 aligns in the following genomic locations:
Homology
BLAST of SMED30000404 vs. TrEMBL
Match: A0A183VJE0 (Uncharacterized protein OS=Trichobilharzia regenti OX=157069 GN=TRE_LOCUS124 PE=4 SV=1) HSP 1 Score: 74.7146 bits (182), Expect = 7.292e-11 Identity = 30/81 (37.04%), Postives = 56/81 (69.14%), Query Frame = 1 Query: 616 AQNVIERVSKKFTDVKLQYTEDFDLDVKILKTENGPK-LSGLFICHLCRKFKGQTKSIKFSVSKENYVILSNVITHLKLHF 855 + + I S++F + L DFD+++++ T +G K ++G+F CHLCR+ + +T +++FS+++ Y +LSNV++HLK HF Sbjct: 441 SASAIRLASRQFVNAYLMRGRDFDMEMELSVTPDGSKRVTGIFYCHLCREKRERTSAVRFSIARNRYPVLSNVLSHLKTHF 521
BLAST of SMED30000404 vs. TrEMBL
Match: A0A0X3Q066 (Uncharacterized protein OS=Schistocephalus solidus OX=70667 GN=TR153293 PE=4 SV=1) HSP 1 Score: 74.7146 bits (182), Expect = 7.560e-11 Identity = 32/101 (31.68%), Postives = 60/101 (59.41%), Query Frame = 1 Query: 556 PNFQSPLSEIAKKAEILNTWAQNVIERVSKKFTDVKLQYTEDFDLDVKILKTENGPK-LSGLFICHLCRKFKGQTKSIKFSVSKENYVILSNVITHLKLHF 855 P FQ + ++ + + +++F + KL EDFD+ +++ G + ++G+F CHLCR+ + +T +++FSV++ Y +LSNVI+HL+ HF Sbjct: 399 PQFQPDTDSNVDLSRLIEHSSASACRLAARQFVNAKLVRGEDFDIQMEVSSPPGGSRRITGIFYCHLCREKRERTCAVRFSVARNRYPVLSNVISHLRTHF 499
BLAST of SMED30000404 vs. TrEMBL
Match: A0A183T8T8 (Uncharacterized protein OS=Schistocephalus solidus OX=70667 GN=SSLN_LOCUS12885 PE=4 SV=1) HSP 1 Score: 74.7146 bits (182), Expect = 8.009e-11 Identity = 32/101 (31.68%), Postives = 60/101 (59.41%), Query Frame = 1 Query: 556 PNFQSPLSEIAKKAEILNTWAQNVIERVSKKFTDVKLQYTEDFDLDVKILKTENGPK-LSGLFICHLCRKFKGQTKSIKFSVSKENYVILSNVITHLKLHF 855 P FQ + ++ + + +++F + KL EDFD+ +++ G + ++G+F CHLCR+ + +T +++FSV++ Y +LSNVI+HL+ HF Sbjct: 392 PQFQPDTDSNVDLSRLIEHSSASACRLAARQFVNAKLVRGEDFDIQMEVSSPPGGSRRITGIFYCHLCREKRERTCAVRFSVARNRYPVLSNVISHLRTHF 492
BLAST of SMED30000404 vs. TrEMBL
Match: A0A1S8WKI8 (Uncharacterized protein (Fragment) OS=Opisthorchis viverrini OX=6198 GN=X801_09246 PE=4 SV=1) HSP 1 Score: 74.3294 bits (181), Expect = 8.043e-11 Identity = 31/81 (38.27%), Postives = 55/81 (67.90%), Query Frame = 1 Query: 616 AQNVIERVSKKFTDVKLQYTEDFDLDVKILKTENGPK-LSGLFICHLCRKFKGQTKSIKFSVSKENYVILSNVITHLKLHF 855 + + I S++F + L DFD++++I T G K ++G+F CHLCR+ + +T +++FS+++ Y +LSNV++HLK HF Sbjct: 169 SASAIRLASRQFLNAHLVRGRDFDMEMEISTTPEGVKRVTGIFYCHLCREKRERTSAVRFSIARNRYPVLSNVLSHLKTHF 249
BLAST of SMED30000404 vs. TrEMBL
Match: A0A074ZZZ3 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_00944 PE=4 SV=1) HSP 1 Score: 74.3294 bits (181), Expect = 8.750e-11 Identity = 31/81 (38.27%), Postives = 55/81 (67.90%), Query Frame = 1 Query: 616 AQNVIERVSKKFTDVKLQYTEDFDLDVKILKTENGPK-LSGLFICHLCRKFKGQTKSIKFSVSKENYVILSNVITHLKLHF 855 + + I S++F + L DFD++++I T G K ++G+F CHLCR+ + +T +++FS+++ Y +LSNV++HLK HF Sbjct: 433 SASAIRLASRQFLNAHLVRGRDFDMEMEISTTPEGVKRVTGIFYCHLCREKRERTSAVRFSIARNRYPVLSNVLSHLKTHF 513
BLAST of SMED30000404 vs. Planmine SMEST
Match: SMESG000012897.1 (SMESG000012897.1) HSP 1 Score: 505.368 bits (1300), Expect = 0.000e+0 Identity = 248/251 (98.80%), Postives = 249/251 (99.20%), Query Frame = 1 Query: 145 MDNFCKSILLDYIKSDKLIFLFEICGVIKINDIMEINDETLNKMEKYIYNFLSHEKNIDHYLDPMFIYRSPEFLFSPGLRMKILRAVKRIKEEYALQNEIQSADKERKISIDQSDEIDNTEAVVNKQVEVIKDKETDPNFQSPLSEIAKKAEILNTWAQNVIERVSKKFTDVKLQYTEDFDLDVKILKTENGPKLSGLFICHLCRKFKGQTKSIKFSVSKENYVILSNVITHLKLHFASVDKLACLPTQLN 897 MDNFCKSILLDY+KSDKLIFLFEICGVIKINDIMEINDETLNKMEKYIYNFLSHEKNIDHYLDPMFIYRSPEFLFSPGLRMKILRAVKRIKEEYALQNEIQSADKERK SIDQSDEIDNTEAVVNKQVE IKDKETDPNFQSPLSEIAKKAEILNTWAQNVIERVSKKFTDVKLQYTEDFDLDVKILKTENGPKLSGLFICHLCRKFKGQTKSIKFSVSKENYVILSNVITHLKLHFASVDKLACLPTQLN Sbjct: 1 MDNFCKSILLDYVKSDKLIFLFEICGVIKINDIMEINDETLNKMEKYIYNFLSHEKNIDHYLDPMFIYRSPEFLFSPGLRMKILRAVKRIKEEYALQNEIQSADKERKTSIDQSDEIDNTEAVVNKQVEEIKDKETDPNFQSPLSEIAKKAEILNTWAQNVIERVSKKFTDVKLQYTEDFDLDVKILKTENGPKLSGLFICHLCRKFKGQTKSIKFSVSKENYVILSNVITHLKLHFASVDKLACLPTQLN 251
BLAST of SMED30000404 vs. Planmine SMEST
Match: SMESG000036851.1 (SMESG000036851.1) HSP 1 Score: 113.235 bits (282), Expect = 2.950e-29 Identity = 72/235 (30.64%), Postives = 122/235 (51.91%), Query Frame = 1 Query: 145 MDNFCKSILLDYIKSDKLIFLFEICGVIKINDIMEINDETLNKMEKYIYN--FLSHEKNIDHYLDPMFIYRSPEFLFSPGLRMKILRAVKRIKEEY------ALQNEIQSADKERKISIDQSDEIDNTEAVV--NKQVEVIKDKETDPN--FQSPLSEIAKKAEILNTWAQNVIERVSKKFTDVKLQYTEDFDLDVKILKTENGPKLSGLFICHLCRKFKGQTKSIKFSVSKENY 813 MD FCK I+L+ S+K+I E+C + + D++ I+D+ ++++E + L + Y+D F FL SPG+R+K+LR+VK I E Y +L N S + + D +V N+ +E I D + N S LS +K + W+++++ R +K F L+ DFD+D++ PK +G+F CH+CR +G + +++FS+SK NY Sbjct: 1 MDEFCKQIILEVTGSEKIIACLELCDIKEDVDVINIDDKVIDRIENFTKECCLLMLPDQMQFYIDERFYNNPSRFLISPGIRIKLLRSVKIITERYQRKTILSLANTYSSMPNNNNNNNASNINDDGNSSVGDNNQFLESISDFKLFNNNLLGSDLSN-EEKVVCVEAWSKSIVHRAAKIFLGANLEPKVDFDMDMETNYQNGVPKRTGIFHCHICRATRGSSATVRFSISKLNY 234
BLAST of SMED30000404 vs. Planmine SMEST
Match: SMESG000009577.1 (SMESG000009577.1) HSP 1 Score: 79.337 bits (194), Expect = 3.286e-16 Identity = 66/305 (21.64%), Postives = 135/305 (44.26%), Query Frame = 1 Query: 145 MDNFCKSILLDYIKSDKLIFLFEICGVIKINDIMEINDETLNKMEKYIYNFLS--HEKNIDHYLDPMFIYRSPEFLFSPGLRMKILRAVKRIKE-------------EYALQ---------------------------------------NEIQSADKERKISIDQSDE--IDNTEAV-VNKQVEVIKDKETDPNFQSPLSEIAKKAEILNT---------WAQNVIERVSKKFTDVKLQYTEDFDLDVKILKT-ENGP-KLSGLFICHLCRKFKGQTKSIKFSVSKENYVILSNVITHLKLHF 855 M+ CK+++ Y++SD +I E+ G I ++ ++++E+++ + + + +L +F + +F F G+RM ++ AV +K E A+Q N++ S K ++DQ+ + +DN E NK + + ++ N EI + + N + + I S++F + L F +D+++ T E GP +++G+F CHLCR+ + ++ ++FS+++ Y ++SNV++HLK+HF Sbjct: 1 METDCKALIDKYVRSDSVIACLELTGYTSAILIRNLDSNQIDQIEEFVRHACTVMNPNQARTFLGSVFSSHADKFCFPIGVRMGLMLAVNELKSLNGNISVNNARLSEKAIQTDISIGCSFSSFLNPNMNFLNKLTATNSIFCDQPQLRLVNDLSSLPSTPKSNVDQASDGFVDNAEMRNTNKLNSSVNNNSSNNNHNETREEIHNRHSMFNEINIEHLKQHSSASAIRLASRQFINANLVQNRHFKIDMEVNPTSEGGPLRVTGIFNCHLCRQKRERSTPVRFSIARNRYPVMSNVLSHLKIHF 305
BLAST of SMED30000404 vs. Planmine SMEST
Match: SMESG000002170.1 (SMESG000002170.1) HSP 1 Score: 67.781 bits (164), Expect = 9.368e-13 Identity = 71/257 (27.63%), Postives = 120/257 (46.69%), Query Frame = 1 Query: 181 IKSDKLIFLFEICGVIKINDIMEINDETLNKMEKYIYN--FLSHEKNIDHYLDPMFIYRSPEFLFSPGLRMKILRAVKRIKEEYALQN------EIQSADKERKI----SIDQSDEIDNTEA---VVNKQVEVIKDKETDPN-------FQSPLS-EIAKKAEILNTWAQNVIERVSKKFTDVKLQYTEDFDLDVKILKTENGPKLS-GLFICHLCRKF-KGQTK-SIKFSVSKENYVILSNVITHLKLHFASVDKL 873 + S K+ ICG ++ N E+L MEKYI FL +++ Y+D +FI EF+F P +IL+ V IKE + N I ++D + + I++SD N + + + I ET N SP+ E K+ + L + Q++ +R ++ F + L+ + D +V++ K ++ S FIC LC K KGQ+ ++F ++ VI N+ HL HF ++K+ Sbjct: 12 LGSSKISACLAICGYTEMQHFASFNTESLANMEKYIGKACFLMNKEERKKYIDEIFIDNPKEFIFPPRCTDEILKIVASIKEYLDMNNVPMKNTNISNSDLDDSLKLIPKIEKSDYFINLKQQSHLFKSIISPIMLNETISNEMSINSCCSSPVCEERIKEIDRLKLFYQSIKDRSNRHFLNCNLE--PNIDFEVQLYKVDDVLYNSKAYFICKLCNKIRKGQSGVVVRFDITPSGNVIFRNIKIHLAKHFQILNKV 266
BLAST of SMED30000404 vs. Planmine SMEST
Match: SMESG000047524.1 (SMESG000047524.1) HSP 1 Score: 67.781 bits (164), Expect = 1.384e-12 Identity = 67/257 (26.07%), Postives = 123/257 (47.86%), Query Frame = 1 Query: 145 MDNFCKSILLDYIKSDKLIFLFEICGVIKINDIMEINDETLNKMEKYIYNFLSHEKNIDH--YLDPMFIYRSPEFLFSPGLRMKILRAVKRI-KEEYALQNEI-QSADKE-RKISIDQSDEIDNTEAVVNKQVEV-IKDKETDPNFQ-SPLSEIAKKAEI----------LNTWAQNVIERVSKKFTDVKLQYTEDFDLDVKILKTENGPKLSGLFICHLCRKFKGQTKSIKFSVSKENYVILSNVITHLKLHFASV 864 M++ + + + ++S ++ EI +++ +D + + ++ +E YI S + D YL FI+ +F+F+PG+R ++L+A K I K+ YA E Q++D E ++S D ++ + Q + I++ + F SPL+ K E+ + + ++++ +K F + L DF L + + L G + CHLC K IKF SK N VI +N+ +HLK H+ V Sbjct: 1 MNSRVRETIANVVESPLVLSCLEIAELLEPDDFINFSHTNVDILENYIRCRTSVMEINDRIPYLSGEFIHFPEKFMFTPGMRNRLLKAAKLISKDVYAAVFESNQTSDCESNEMSCDSNNNSTTPMNKLQPQNNISIENSLIENQFLLSPLNSSIIKQELDMNRSRHLQNMEKYYNDIVKNAAKNFIECSLIPDVDFHLSIP---ENDSTDLIGYYHCHLCTDRKKAMTKIKFRFSKYNSVIYTNINSHLKTHYQYV 254 The following BLAST results are available for this feature:
BLAST of SMED30000404 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30000404 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30000404 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30000404 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30000404 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30000404 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30000404 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30000404 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30000404 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30000404 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30000404 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30000404 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30000404 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30000404 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30000404 ID=SMED30000404|Name=SMED30000404|organism=Schmidtea mediterranea sexual|type=transcript|length=1048bpback to top protein sequence of SMED30000404-orf-1 >SMED30000404-orf-1 ID=SMED30000404-orf-1|Name=SMED30000404-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=252bp MDNFCKSILLDYIKSDKLIFLFEICGVIKINDIMEINDETLNKMEKYIYNback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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