SMED30000404

Overview
NameSMED30000404
Smed IDSMED30000404
Length (bp)1048
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30000404 (SMED30000404) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30000404 (SMED30000404) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30000404 (SMED30000404) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30000404 (SMED30000404) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30000404

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
protonephridiaSMED30000404SMESG000012897.1 dd_Smed_v4_6875_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30000404SMESG000012897.1 dd_Smed_v4_6875_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30000404SMESG000012897.1 dd_Smed_v4_6875_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior sideSMED30000404SMESG000012897.1 dd_Smed_v4_6875_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30000404SMESG000012897.1 dd_Smed_v4_6875_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30000404 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.004164:20989..22080 -5151
Homology
BLAST of SMED30000404 vs. TrEMBL
Match: A0A183VJE0 (Uncharacterized protein OS=Trichobilharzia regenti OX=157069 GN=TRE_LOCUS124 PE=4 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 7.292e-11
Identity = 30/81 (37.04%), Postives = 56/81 (69.14%), Query Frame = 1
Query:  616 AQNVIERVSKKFTDVKLQYTEDFDLDVKILKTENGPK-LSGLFICHLCRKFKGQTKSIKFSVSKENYVILSNVITHLKLHF 855
            + + I   S++F +  L    DFD+++++  T +G K ++G+F CHLCR+ + +T +++FS+++  Y +LSNV++HLK HF
Sbjct:  441 SASAIRLASRQFVNAYLMRGRDFDMEMELSVTPDGSKRVTGIFYCHLCREKRERTSAVRFSIARNRYPVLSNVLSHLKTHF 521          
BLAST of SMED30000404 vs. TrEMBL
Match: A0A0X3Q066 (Uncharacterized protein OS=Schistocephalus solidus OX=70667 GN=TR153293 PE=4 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 7.560e-11
Identity = 32/101 (31.68%), Postives = 60/101 (59.41%), Query Frame = 1
Query:  556 PNFQSPLSEIAKKAEILNTWAQNVIERVSKKFTDVKLQYTEDFDLDVKILKTENGPK-LSGLFICHLCRKFKGQTKSIKFSVSKENYVILSNVITHLKLHF 855
            P FQ         + ++   + +     +++F + KL   EDFD+ +++     G + ++G+F CHLCR+ + +T +++FSV++  Y +LSNVI+HL+ HF
Sbjct:  399 PQFQPDTDSNVDLSRLIEHSSASACRLAARQFVNAKLVRGEDFDIQMEVSSPPGGSRRITGIFYCHLCREKRERTCAVRFSVARNRYPVLSNVISHLRTHF 499          
BLAST of SMED30000404 vs. TrEMBL
Match: A0A183T8T8 (Uncharacterized protein OS=Schistocephalus solidus OX=70667 GN=SSLN_LOCUS12885 PE=4 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 8.009e-11
Identity = 32/101 (31.68%), Postives = 60/101 (59.41%), Query Frame = 1
Query:  556 PNFQSPLSEIAKKAEILNTWAQNVIERVSKKFTDVKLQYTEDFDLDVKILKTENGPK-LSGLFICHLCRKFKGQTKSIKFSVSKENYVILSNVITHLKLHF 855
            P FQ         + ++   + +     +++F + KL   EDFD+ +++     G + ++G+F CHLCR+ + +T +++FSV++  Y +LSNVI+HL+ HF
Sbjct:  392 PQFQPDTDSNVDLSRLIEHSSASACRLAARQFVNAKLVRGEDFDIQMEVSSPPGGSRRITGIFYCHLCREKRERTCAVRFSVARNRYPVLSNVISHLRTHF 492          
BLAST of SMED30000404 vs. TrEMBL
Match: A0A1S8WKI8 (Uncharacterized protein (Fragment) OS=Opisthorchis viverrini OX=6198 GN=X801_09246 PE=4 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 8.043e-11
Identity = 31/81 (38.27%), Postives = 55/81 (67.90%), Query Frame = 1
Query:  616 AQNVIERVSKKFTDVKLQYTEDFDLDVKILKTENGPK-LSGLFICHLCRKFKGQTKSIKFSVSKENYVILSNVITHLKLHF 855
            + + I   S++F +  L    DFD++++I  T  G K ++G+F CHLCR+ + +T +++FS+++  Y +LSNV++HLK HF
Sbjct:  169 SASAIRLASRQFLNAHLVRGRDFDMEMEISTTPEGVKRVTGIFYCHLCREKRERTSAVRFSIARNRYPVLSNVLSHLKTHF 249          
BLAST of SMED30000404 vs. TrEMBL
Match: A0A074ZZZ3 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_00944 PE=4 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 8.750e-11
Identity = 31/81 (38.27%), Postives = 55/81 (67.90%), Query Frame = 1
Query:  616 AQNVIERVSKKFTDVKLQYTEDFDLDVKILKTENGPK-LSGLFICHLCRKFKGQTKSIKFSVSKENYVILSNVITHLKLHF 855
            + + I   S++F +  L    DFD++++I  T  G K ++G+F CHLCR+ + +T +++FS+++  Y +LSNV++HLK HF
Sbjct:  433 SASAIRLASRQFLNAHLVRGRDFDMEMEISTTPEGVKRVTGIFYCHLCREKRERTSAVRFSIARNRYPVLSNVLSHLKTHF 513          
BLAST of SMED30000404 vs. Planmine SMEST
Match: SMESG000012897.1 (SMESG000012897.1)

HSP 1 Score: 505.368 bits (1300), Expect = 0.000e+0
Identity = 248/251 (98.80%), Postives = 249/251 (99.20%), Query Frame = 1
Query:  145 MDNFCKSILLDYIKSDKLIFLFEICGVIKINDIMEINDETLNKMEKYIYNFLSHEKNIDHYLDPMFIYRSPEFLFSPGLRMKILRAVKRIKEEYALQNEIQSADKERKISIDQSDEIDNTEAVVNKQVEVIKDKETDPNFQSPLSEIAKKAEILNTWAQNVIERVSKKFTDVKLQYTEDFDLDVKILKTENGPKLSGLFICHLCRKFKGQTKSIKFSVSKENYVILSNVITHLKLHFASVDKLACLPTQLN 897
            MDNFCKSILLDY+KSDKLIFLFEICGVIKINDIMEINDETLNKMEKYIYNFLSHEKNIDHYLDPMFIYRSPEFLFSPGLRMKILRAVKRIKEEYALQNEIQSADKERK SIDQSDEIDNTEAVVNKQVE IKDKETDPNFQSPLSEIAKKAEILNTWAQNVIERVSKKFTDVKLQYTEDFDLDVKILKTENGPKLSGLFICHLCRKFKGQTKSIKFSVSKENYVILSNVITHLKLHFASVDKLACLPTQLN
Sbjct:    1 MDNFCKSILLDYVKSDKLIFLFEICGVIKINDIMEINDETLNKMEKYIYNFLSHEKNIDHYLDPMFIYRSPEFLFSPGLRMKILRAVKRIKEEYALQNEIQSADKERKTSIDQSDEIDNTEAVVNKQVEEIKDKETDPNFQSPLSEIAKKAEILNTWAQNVIERVSKKFTDVKLQYTEDFDLDVKILKTENGPKLSGLFICHLCRKFKGQTKSIKFSVSKENYVILSNVITHLKLHFASVDKLACLPTQLN 251          
BLAST of SMED30000404 vs. Planmine SMEST
Match: SMESG000036851.1 (SMESG000036851.1)

HSP 1 Score: 113.235 bits (282), Expect = 2.950e-29
Identity = 72/235 (30.64%), Postives = 122/235 (51.91%), Query Frame = 1
Query:  145 MDNFCKSILLDYIKSDKLIFLFEICGVIKINDIMEINDETLNKMEKYIYN--FLSHEKNIDHYLDPMFIYRSPEFLFSPGLRMKILRAVKRIKEEY------ALQNEIQSADKERKISIDQSDEIDNTEAVV--NKQVEVIKDKETDPN--FQSPLSEIAKKAEILNTWAQNVIERVSKKFTDVKLQYTEDFDLDVKILKTENGPKLSGLFICHLCRKFKGQTKSIKFSVSKENY 813
            MD FCK I+L+   S+K+I   E+C + +  D++ I+D+ ++++E +      L     +  Y+D  F      FL SPG+R+K+LR+VK I E Y      +L N   S       +   +   D   +V   N+ +E I D +   N    S LS   +K   +  W+++++ R +K F    L+   DFD+D++       PK +G+F CH+CR  +G + +++FS+SK NY
Sbjct:    1 MDEFCKQIILEVTGSEKIIACLELCDIKEDVDVINIDDKVIDRIENFTKECCLLMLPDQMQFYIDERFYNNPSRFLISPGIRIKLLRSVKIITERYQRKTILSLANTYSSMPNNNNNNNASNINDDGNSSVGDNNQFLESISDFKLFNNNLLGSDLSN-EEKVVCVEAWSKSIVHRAAKIFLGANLEPKVDFDMDMETNYQNGVPKRTGIFHCHICRATRGSSATVRFSISKLNY 234          
BLAST of SMED30000404 vs. Planmine SMEST
Match: SMESG000009577.1 (SMESG000009577.1)

HSP 1 Score: 79.337 bits (194), Expect = 3.286e-16
Identity = 66/305 (21.64%), Postives = 135/305 (44.26%), Query Frame = 1
Query:  145 MDNFCKSILLDYIKSDKLIFLFEICGVIKINDIMEINDETLNKMEKYIYNFLS--HEKNIDHYLDPMFIYRSPEFLFSPGLRMKILRAVKRIKE-------------EYALQ---------------------------------------NEIQSADKERKISIDQSDE--IDNTEAV-VNKQVEVIKDKETDPNFQSPLSEIAKKAEILNT---------WAQNVIERVSKKFTDVKLQYTEDFDLDVKILKT-ENGP-KLSGLFICHLCRKFKGQTKSIKFSVSKENYVILSNVITHLKLHF 855
            M+  CK+++  Y++SD +I   E+ G      I  ++   ++++E+++ +  +  +      +L  +F   + +F F  G+RM ++ AV  +K              E A+Q                                       N++ S     K ++DQ+ +  +DN E    NK    + +  ++ N      EI  +  + N           + + I   S++F +  L     F +D+++  T E GP +++G+F CHLCR+ + ++  ++FS+++  Y ++SNV++HLK+HF
Sbjct:    1 METDCKALIDKYVRSDSVIACLELTGYTSAILIRNLDSNQIDQIEEFVRHACTVMNPNQARTFLGSVFSSHADKFCFPIGVRMGLMLAVNELKSLNGNISVNNARLSEKAIQTDISIGCSFSSFLNPNMNFLNKLTATNSIFCDQPQLRLVNDLSSLPSTPKSNVDQASDGFVDNAEMRNTNKLNSSVNNNSSNNNHNETREEIHNRHSMFNEINIEHLKQHSSASAIRLASRQFINANLVQNRHFKIDMEVNPTSEGGPLRVTGIFNCHLCRQKRERSTPVRFSIARNRYPVMSNVLSHLKIHF 305          
BLAST of SMED30000404 vs. Planmine SMEST
Match: SMESG000002170.1 (SMESG000002170.1)

HSP 1 Score: 67.781 bits (164), Expect = 9.368e-13
Identity = 71/257 (27.63%), Postives = 120/257 (46.69%), Query Frame = 1
Query:  181 IKSDKLIFLFEICGVIKINDIMEINDETLNKMEKYIYN--FLSHEKNIDHYLDPMFIYRSPEFLFSPGLRMKILRAVKRIKEEYALQN------EIQSADKERKI----SIDQSDEIDNTEA---VVNKQVEVIKDKETDPN-------FQSPLS-EIAKKAEILNTWAQNVIERVSKKFTDVKLQYTEDFDLDVKILKTENGPKLS-GLFICHLCRKF-KGQTK-SIKFSVSKENYVILSNVITHLKLHFASVDKL 873
            + S K+     ICG  ++      N E+L  MEKYI    FL +++    Y+D +FI    EF+F P    +IL+ V  IKE   + N       I ++D +  +     I++SD   N +    +    +  I   ET  N         SP+  E  K+ + L  + Q++ +R ++ F +  L+   + D +V++ K ++    S   FIC LC K  KGQ+   ++F ++    VI  N+  HL  HF  ++K+
Sbjct:   12 LGSSKISACLAICGYTEMQHFASFNTESLANMEKYIGKACFLMNKEERKKYIDEIFIDNPKEFIFPPRCTDEILKIVASIKEYLDMNNVPMKNTNISNSDLDDSLKLIPKIEKSDYFINLKQQSHLFKSIISPIMLNETISNEMSINSCCSSPVCEERIKEIDRLKLFYQSIKDRSNRHFLNCNLE--PNIDFEVQLYKVDDVLYNSKAYFICKLCNKIRKGQSGVVVRFDITPSGNVIFRNIKIHLAKHFQILNKV 266          
BLAST of SMED30000404 vs. Planmine SMEST
Match: SMESG000047524.1 (SMESG000047524.1)

HSP 1 Score: 67.781 bits (164), Expect = 1.384e-12
Identity = 67/257 (26.07%), Postives = 123/257 (47.86%), Query Frame = 1
Query:  145 MDNFCKSILLDYIKSDKLIFLFEICGVIKINDIMEINDETLNKMEKYIYNFLSHEKNIDH--YLDPMFIYRSPEFLFSPGLRMKILRAVKRI-KEEYALQNEI-QSADKE-RKISIDQSDEIDNTEAVVNKQVEV-IKDKETDPNFQ-SPLSEIAKKAEI----------LNTWAQNVIERVSKKFTDVKLQYTEDFDLDVKILKTENGPKLSGLFICHLCRKFKGQTKSIKFSVSKENYVILSNVITHLKLHFASV 864
            M++  +  + + ++S  ++   EI  +++ +D +  +   ++ +E YI    S  +  D   YL   FI+   +F+F+PG+R ++L+A K I K+ YA   E  Q++D E  ++S D ++        +  Q  + I++   +  F  SPL+    K E+          +  +  ++++  +K F +  L    DF L +      +   L G + CHLC   K     IKF  SK N VI +N+ +HLK H+  V
Sbjct:    1 MNSRVRETIANVVESPLVLSCLEIAELLEPDDFINFSHTNVDILENYIRCRTSVMEINDRIPYLSGEFIHFPEKFMFTPGMRNRLLKAAKLISKDVYAAVFESNQTSDCESNEMSCDSNNNSTTPMNKLQPQNNISIENSLIENQFLLSPLNSSIIKQELDMNRSRHLQNMEKYYNDIVKNAAKNFIECSLIPDVDFHLSIP---ENDSTDLIGYYHCHLCTDRKKAMTKIKFRFSKYNSVIYTNINSHLKTHYQYV 254          
The following BLAST results are available for this feature:
BLAST of SMED30000404 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000404 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000404 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000404 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000404 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000404 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000404 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000404 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A183VJE07.292e-1137.04Uncharacterized protein OS=Trichobilharzia regenti... [more]
A0A0X3Q0667.560e-1131.68Uncharacterized protein OS=Schistocephalus solidus... [more]
A0A183T8T88.009e-1131.68Uncharacterized protein OS=Schistocephalus solidus... [more]
A0A1S8WKI88.043e-1138.27Uncharacterized protein (Fragment) OS=Opisthorchis... [more]
A0A074ZZZ38.750e-1138.27Uncharacterized protein OS=Opisthorchis viverrini ... [more]
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BLAST of SMED30000404 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000404 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000404 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000404 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000404 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000404 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000012897.10.000e+098.80SMESG000012897.1[more]
SMESG000036851.12.950e-2930.64SMESG000036851.1[more]
SMESG000009577.13.286e-1621.64SMESG000009577.1[more]
SMESG000002170.19.368e-1327.63SMESG000002170.1[more]
SMESG000047524.11.384e-1226.07SMESG000047524.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30000404 ID=SMED30000404|Name=SMED30000404|organism=Schmidtea mediterranea sexual|type=transcript|length=1048bp
AATTACAAATTATTTTATTTATGCTGTTTTATCAAGTTTTTGAATCCTAT
AATTTTATATTTCACTAATTATAATAAAATTTCGACTAATTTCGATTATT
AATAATAATTTTATGATTATCTTTATTAAATTAAGGAGCGCTTAATGGAT
AACTTTTGCAAAAGTATTCTTCTGGATTATATAAAATCAGATAAACTTAT
TTTCCTATTTGAAATTTGTGGCGTCATTAAGATAAATGATATAATGGAAA
TCAATGATGAAACATTAAACAAGATGGAAAAATATATTTATAATTTCTTG
TCTCATGAAAAAAATATCGACCATTATTTAGACCCAATGTTTATCTACAG
ATCTCCAGAATTTTTGTTTTCTCCTGGCTTAAGAATGAAAATACTTCGAG
CAGTTAAACGAATTAAAGAAGAATATGCATTGCAAAATGAAATTCAAAGC
GCTGACAAGGAAAGAAAAATTTCTATTGATCAATCTGATGAAATTGATAA
TACCGAAGCTGTAGTTAATAAACAAGTTGAAGTAATAAAAGATAAGGAAA
CTGATCCTAATTTTCAATCCCCGCTAAGTGAAATTGCTAAAAAAGCCGAA
ATTCTCAATACTTGGGCACAAAATGTGATTGAAAGAGTTTCTAAAAAATT
TACCGATGTTAAATTGCAATACACTGAAGATTTTGATTTAGATGTAAAAA
TTTTAAAAACCGAAAATGGTCCCAAATTGAGTGGTCTGTTTATATGTCAT
CTGTGTAGAAAATTTAAAGGTCAAACCAAAAGCATTAAATTTAGTGTTTC
CAAGGAAAATTACGTTATTTTAAGTAATGTTATTACCCATCTGAAATTGC
ATTTTGCGTCTGTTGATAAATTAGCTTGTCTTCCCACTCAGCTAAATTGA
TTTCATAATTCTGTATCATATTTTTGCGGTTCAGTTATTAGGATTTTCTA
ACAGTTTAGTCATTTTTTGACAATATATTAATTTTCTAACATTTGTAAAT
CAATTTTGTAACGAAACATTTTCTTTTTTAATAAATGCTTTAATATCT
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protein sequence of SMED30000404-orf-1

>SMED30000404-orf-1 ID=SMED30000404-orf-1|Name=SMED30000404-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=252bp
MDNFCKSILLDYIKSDKLIFLFEICGVIKINDIMEINDETLNKMEKYIYN
FLSHEKNIDHYLDPMFIYRSPEFLFSPGLRMKILRAVKRIKEEYALQNEI
QSADKERKISIDQSDEIDNTEAVVNKQVEVIKDKETDPNFQSPLSEIAKK
AEILNTWAQNVIERVSKKFTDVKLQYTEDFDLDVKILKTENGPKLSGLFI
CHLCRKFKGQTKSIKFSVSKENYVILSNVITHLKLHFASVDKLACLPTQL
N*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 117..137