SMED30000394
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppSub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30000394 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Alignments
SMED30000394 aligns in the following genomic locations:
Homology
BLAST of SMED30000394 vs. Planmine SMEST
Match: SMESG000025344.1 (SMESG000025344.1) HSP 1 Score: 869.381 bits (2245), Expect = 0.000e+0 Identity = 471/490 (96.12%), Postives = 482/490 (98.37%), Query Frame = -2 Query: 162 MESGGYFLNQRKEICLKFLISVPLFLFGMVYVTNYGFYVYQISPRGDRKDEYLDKINYSISFVKIVSIILICYNTQINHRFRNLRIKHKLEQPLIFYLICLPILLMSLIGVFVVILYYTFERSVKNPSHITYCSLVIFEYFLFIVLVIDINGFSDTGEEWFMNYFNSXXXXXXXXXXXXXICLNIIMILTDDFTNTMNRTTFVSLFSNHNFTIFDKTCYRTLVFLKKRAVITLSLVFIPFLVIPLKRNLTHLFRFKPKEDLPEDKTVLSMNKVSSITGGFTVGVFLTFIVIVHLLYVMQNVPQVIIASRIIKLLVSFFISCLLGIMITEIKISARLNSSQYLNVKNILIYLGAFGQFSVQITNIVVNIYNLQTNGNGLFESTKDILILTSNFVYXXXXXXXXXXXLFVLDGKAVSFPKNSWSIFFYSPINILILQIGVFTEYLTKSFYDEQNSFQIRMMEIGTMYFAIPLVILYRAICFIIILLILRPFK 1631 MESGGYFLNQRKEICLKFLISVPLFLFGMVYVTNYGFYVYQISPRGDRKDEYLDKINYSISFVKIVSIILICYNTQINHRFRNLRIKHKLEQPLIFYLICLP+L +SL+G+FV+I +YTFE+S ++I Y +LVIFEYFLFI+LVIDINGFSDTGEEWFMNYFNSMTIIITMTSVMIQICLNIIMILTDDFTNTMNRTTFVSLFSNHNFTIFDKTCYRTLVFLKKRAVITLSLVFIPFLVIPLKRNLTHLFRFKPKEDLPEDKTVLSMNKVSSITGGFTVGVFLTFIVIVHLLYVMQNVPQVIIASRIIKLLVSFFISCLLGIMITEIKISARLNSSQYLNVKNILIYLGAFGQFSVQITNIVVNIYNLQTNGNGLFESTKDILILTSNFVYLIETILMTTLMLFVLDGKAVSFPKNSWSIFFYSPINILILQIGVFTEYLTKSFYDEQNSFQIRMMEIGTMYFAIPLVILYRAICFIIILLILRPFK Sbjct: 1 MESGGYFLNQRKEICLKFLISVPLFLFGMVYVTNYGFYVYQISPRGDRKDEYLDKINYSISFVKIVSIILICYNTQINHRFRNLRIKHKLEQPLIFYLICLPMLFISLMGLFVIIFHYTFEKS-GTSTNILYFALVIFEYFLFIILVIDINGFSDTGEEWFMNYFNSMTIIITMTSVMIQICLNIIMILTDDFTNTMNRTTFVSLFSNHNFTIFDKTCYRTLVFLKKRAVITLSLVFIPFLVIPLKRNLTHLFRFKPKEDLPEDKTVLSMNKVSSITGGFTVGVFLTFIVIVHLLYVMQNVPQVIIASRIIKLLVSFFISCLLGIMITEIKISARLNSSQYLNVKNILIYLGAFGQFSVQITNIVVNIYNLQTNGNGLFESTKDILILTSNFVYLIETILMTTLMLFVLDGKAVSFPKNSWSIFFYSPINILILQIGVFTEYLTKSFYDEQNSFQIRMMEIGTMYFAIPLVILYRAICFIIILLILRPFK 489
BLAST of SMED30000394 vs. Planmine SMEST
Match: SMESG000025336.1 (SMESG000025336.1) HSP 1 Score: 688.337 bits (1775), Expect = 0.000e+0 Identity = 370/492 (75.20%), Postives = 429/492 (87.20%), Query Frame = -2 Query: 162 MESGGYFLNQRKEICLKFLISVPLFLFGMVYVTNYGFYVYQISPRGDRKDEYLDKINYSISFVKIVSIILICYNTQINHRFRNLRIKHKLEQPLIFYLICLPILLMSLIGVFVVILYYTFERSVKNPSHITYCSLVIFEYFLFIVLVIDINGFSDTGEEWFMNYFNSXXXXXXXXXXXXXICLNIIMILTDDFTNTMNRTTFVSLFSNHNFTIFDKTCYRTLVFLKKRAVITLSLVFIPFLVIPLKRNLTHLFRFKPKEDLPEDKTVLSMNKVSSITGGFTVGVFLTFIVIVHL--LYVMQNVPQVIIASRIIKLLVSFFISCLLGIMITEIKISARLNSSQYLNVKNILIYLGAFGQFSVQITNIVVNIYNLQTNGNGLFESTKDILILTSNFVYXXXXXXXXXXXLFVLDGKAVSFPKNSWSIFFYSPINILILQIGVFTEYLTKSFYDEQNSFQIRMMEIGTMYFAIPLVILYRAICFIIILLILRPFK 1631 MESGGYFLNQRKEICLKFLISVPLFLFGMVYVTNYGFYVY+I+P +R D Y++KINYSISFVK+VSIILICYNT+INHRFRNLRIKHKLEQPLIFYLICLPILLMSLIGVFVVILYYTFERSVKNPSHITYCSLVIFEYFLFIVLVIDINGFSDTGEEWFMNYFNSMTIIITMTSV++QICLNIIMILT++F+N++N+ +L+SNH+FT FDKT Y ++FLKKR VITLSL FIPFLVIPLKRNL HLF+FK K+DLPED+ +LSMNKVSSITG TVGV++T I IV L + + + + SRIIK + F LL IMITEIK+SARLNSSQYLNVKNILIYLGAFGQFS+ I IVV+IYNL+ NG GLFE ++IL+L S+F YLIET+L+TTLMLFVLDG+ + F +NSW+ FF++PINILILQ+ +F+EYLTK Y ++NSFQIR++E GT+ FAI LV LYR+ICF+IIL +L+PFK Sbjct: 1 MESGGYFLNQRKEICLKFLISVPLFLFGMVYVTNYGFYVYRITPNENRNDGYIEKINYSISFVKVVSIILICYNTKINHRFRNLRIKHKLEQPLIFYLICLPILLMSLIGVFVVILYYTFERSVKNPSHITYCSLVIFEYFLFIVLVIDINGFSDTGEEWFMNYFNSMTIIITMTSVIMQICLNIIMILTEEFSNSLNQVLHFNLYSNHSFTFFDKTSYNKIIFLKKRTVITLSLAFIPFLVIPLKRNLNHLFQFKTKDDLPEDQPILSMNKVSSITGSLTVGVYITLIFIVDLCNFVIHERGNYIYLTSRIIKAFLILFSIGLLAIMITEIKVSARLNSSQYLNVKNILIYLGAFGQFSISIAYIVVDIYNLEINGIGLFELIQNILLLVSSFEYLIETMLLTTLMLFVLDGETIFFLENSWTFFFHTPINILILQLSIFSEYLTKPVYGKENSFQIRIIEQGTLDFAIYLVYLYRSICFMIILFLLKPFK 492
BLAST of SMED30000394 vs. Planmine SMEST
Match: SMESG000025342.1 (SMESG000025342.1) HSP 1 Score: 506.908 bits (1304), Expect = 2.030e-178 Identity = 269/341 (78.89%), Postives = 299/341 (87.68%), Query Frame = -2 Query: 615 MESGGYFLNQRKEICLKFLISVPLFLFGMVYVTNYGFYVYQISPRGDRKDEYLDKINYSISFVKIVSIILICYNTQINHRFRNLRIKHKLEQPLIFYLICLPILLMSLIGVFVVILYYTFERSVKNPSHITYCSLVIFEYFLFIVLVIDINGFSDTGEEWFMNYFNSXXXXXXXXXXXXXICLNIIMILTDDFTNTMNRTTFVSLFSNHNFTIFDKTCYRTLVFLKKRAVITLSLVFIPFLVIPLKRNLTHLFRFKPKEDLPEDKTVLSMNKVSSITGGFTVGVFLTFIVIVHL--LYVMQNVPQVIIASRIIKLLVSFFISCLLGIMITEIKISARLNSS 1631 MESGGYFLNQRKEICLKFLISVPLFLFGMVYVTNYGFYVY+ISP +R D Y++KINYSISFVK+VSIILICYNT+INHRFRNLRIKHKL+QPLIFYLICLPILLMSLIGVFVVILYYTFERSVKNPSHITYCSLVIFEYFLFIVLVIDINGFSDTGEEWFMNYFNSMTIIITMTSV++QICLNIIMILTD+F N++N+ +L+SNH+FT FDKT Y ++FLKKR VITLSL FIPFLVIPLKRNL HLF+FK K+DLPED+ +LSMNKVSSITG TVGV++T I IV L + + + + SRIIK + F LL IMITEIK+SARLNSS Sbjct: 1 MESGGYFLNQRKEICLKFLISVPLFLFGMVYVTNYGFYVYRISPMENRNDGYIEKINYSISFVKVVSIILICYNTKINHRFRNLRIKHKLKQPLIFYLICLPILLMSLIGVFVVILYYTFERSVKNPSHITYCSLVIFEYFLFIVLVIDINGFSDTGEEWFMNYFNSMTIIITMTSVIMQICLNIIMILTDEFFNSLNQVLHFNLYSNHSFTFFDKTSYNKIIFLKKRTVITLSLAFIPFLVIPLKRNLNHLFQFKTKDDLPEDQPILSMNKVSSITGSLTVGVYITLIFIVDLCNFVIHERGNYIYLTSRIIKAFLILFSIGLLAIMITEIKVSARLNSS 341
BLAST of SMED30000394 vs. Planmine SMEST
Match: SMESG000025346.1 (SMESG000025346.1) HSP 1 Score: 379.407 bits (973), Expect = 3.384e-126 Identity = 247/499 (49.50%), Postives = 338/499 (67.74%), Query Frame = -2 Query: 162 MESGGYFLNQRKEICLKFLISVPLFLFGMVYVTNYGFYVYQISPRGDRKDEYLDKINYSISFVKIVSIILICYNTQINHRFRNLRIKHKLEQPLIFYLICLPILLMSLIGVFVVILYYTFERSVKNPSHITYCSLVIFEYFLFIVLVIDINGFSDTGEEWFMNYFNSXXXXXXXXXXXXXICLNIIMILTDDFTNTMNRTTFVSLFSNHNFTIFDKTCYRTLVFLKKRAVITLSLVFIPFLVIPLKRNLTHLFRFKPKEDLPEDKTVLSMNKVSSITGGFTVGVFLTFIVIVHLLYVMQNVPQ--VIIASRIIKLLVSFFISCLLGIMITEIKISARLNSSQYLNVKNILIYLGAFGQFSVQITNIVVNIYNL---QTNGNGLFESTKDILILTSNFVYXXXXXXXXXXXLFVLDGKAVSFPKNSWSIFFYSPINILILQIGV----FTEYLTKSFYDEQNSFQIRMMEIGTMYFAIPLVILYRAICFIIILLILRPFK 1631 ME +F N +KEI LK LIS+PL + +V+VTNYG YVY ++ G++ Y INYS+ VKI SI+LICYN QIN+RFRNLRIKHKL QPL FYLICLP++L+S+IG+ V+ L+ F+R++ + S YC LVI EYFL+IVL+IDI+GF DTGEEW MNY NSMTIIIT+TS+ +QIC NII++LTD F+ T+NR +N + ++ ++ KKRAVITL VFIPFL+I L+ NL HL RFK K + +++ V ++N+++ TGG T+ ++L + V L++V++ Q + A RIIK+ + F LL IMI E+KIS+RL S YL+VK+ILIYLG GQF I NI++ IY + + N FE+ I++ T + + + T + L+ DGK++ F +NSW+ FFYSP+NILILQI + F + K+ Y NSFQI +++ GT+ FAIPLV LYR IC II + IL PFK Sbjct: 1 MEQNVHFNNHKKEIYLKLLISLPLLVMSLVFVTNYGLYVYMVTLEGNKNIAYDFMINYSMCLVKIFSIMLICYNRQINYRFRNLRIKHKLIQPLTFYLICLPMILVSIIGLVVLSLHEIFDRNLDDVSRFIYCCLVILEYFLYIVLIIDISGFRDTGEEWIMNYVNSMTIIITITSIAMQICYNIILLLTDCFSKTLNRPE-----NNKTYAFINRPLLVRIMDNKKRAVITLIAVFIPFLLISLQVNLNHLIRFKKKLESLKEEIVPNINQITFATGGLTIVIYLALTITVDLVHVLEKDHQRNIFAAGRIIKVFLVLFSIFLLVIMIIELKISSRLKSPNYLSVKSILIYLGTLGQFCSLIMNIMITIYKINAKEVNSEKRFETFLSIVLDTESLLEVTLTTTLVLLLS---DGKSIFFSRNSWTFFFYSPVNILILQISICFQNFGKINNKNVY---NSFQIDLIKHGTLTFAIPLVFLYRLICIIIFISILPPFK 488
BLAST of SMED30000394 vs. Planmine SMEST
Match: SMESG000025369.1 (SMESG000025369.1) HSP 1 Score: 142.895 bits (359), Expect = 1.712e-36 Identity = 138/500 (27.60%), Postives = 251/500 (50.20%), Query Frame = -2 Query: 186 AMESGGYFLNQRKEICLKFLISVPLFLFGMVYVTNYGFYVYQISPRGDRKDEYLDKINYSISFVKIVSIILICYNTQINHRFRNLRIKHKLEQPLIFYLICLPILLMSLIGVFVVILYYTFERSVKNPSHITYCSLVIFEYFLFIVLVIDINGFSDTGEEWFMNYFNSXXXXXXXXXXXXXICLNIIMILTDDFTNTMNRTTFVSLFSNHNF--TIFDKTCYRTLVFLKKRAVITLSLVFIPFLVIPLKRNLTHLFRFKPKEDLPEDKTVLSMNKVSSITGGFTVGVF----LTFIVIVH-LLYVMQNVPQVIIASRIIKLLVSFFISCLLGIMITEIKISARLNSSQYLNVKNILIYLGAFGQFSVQITNIVVNIYNLQTNGNGLFESTKDILILTSNFVYXXXXXXXXXXXL-------FVLDGKAVSFPKNSWSIFFYSPINILILQIGVFTEYLTKSFY---DEQNSFQIRMMEIGTMYFAIPLVILYRAICFIII 1634 M + +KF+IS+ L +F +VY+TNY ++ + +P DR I ++ VK+ +I+LI YN +IN+RFRNLRI++ +E+P+I YLICLP+L++S+I + + L T + + I I+ ++DI+G +TGEEW M+Y N IIIT+ ++ ++ + ++ ++N + + +S + + F + + + +++ ++I L +V P+L++ L +++ ++F+ K + P+ +V ++ + S++G TV VF L I+ H Y + I R L F+ + MI EI++ + +++ ++K + IY G FG + + NI+ Q +FE+T L+ N Y+I + + +LD + F K SWS F SPI +L+ ++ E + S Y F+I ++ IG+ FA P+VI Y C II+ Sbjct: 32 TMPQDSIVTQSNRNAVIKFMISIKLLIFSIVYLTNYSLFIVRFTPLKDRCIGIPKIITCAMVIVKLTNILLIIYNARINYRFRNLRIRNPVERPMILYLICLPLLVISIISISLSALNTTLKWLSDSIDMSVDFGCSILNLIAVILFILDISGCRNTGEEWLMSYVNFCIIIITICTIFMEAMKTLTFKMSSIYSNLIYKYFNISFYEENKFSKSFLTSSAINQIRVIEEDSIIMLLVVLFPYLIVCLDQHMKNMFKDVSKGNNPQTLSVRNLTFL-SLSGCITVVVFSIFELVLIIYTHGKPYTEWGCEEWI--YRGFDGLKFIFVVINIVSMIHEIRLWLTVEENRFYDLKKLFIYFGTFGNIIKAVILVFFNIF--QIVSQSVFENTT----LSVNCSYIIMHVEDIIQTVLMSSLILLILDKEPKPFLKISWSYEFCSPILLLMNRVN---EIIRCSLYMLIPNGKYFEINLILIGSYSFAQPIVIFYNVTCVIIL 519 The following BLAST results are available for this feature:
BLAST of SMED30000394 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30000394 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30000394 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30000394 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30000394 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30000394 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30000394 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30000394 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30000394 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30000394 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30000394 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30000394 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30000394 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30000394 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30000394 ID=SMED30000394|Name=SMED30000394|organism=Schmidtea mediterranea sexual|type=transcript|length=1680bpback to top protein sequence of SMED30000394-orf-1 >SMED30000394-orf-1 ID=SMED30000394-orf-1|Name=SMED30000394-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=491bp MESGGYFLNQRKEICLKFLISVPLFLFGMVYVTNYGFYVYQISPRGDRKDback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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