SMED30000388

Overview
NameSMED30000388
Smed IDSMED30000388
Length (bp)459
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30000388 (SMED30000388) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30000388 (SMED30000388) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30000388 (SMED30000388) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30000388 (SMED30000388) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30000388

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parenchymaSMED30000388SMESG000022724.1 dd_Smed_v4_13099_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30000388 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001411:81511..82049 -2238
Homology
[Close]
BLAST of SMED30000388 vs. Ensembl Human
Match: TMEM170A (transmembrane protein 170A [Source:HGNC Symbol;Acc:HGNC:29577])

HSP 1 Score: 50.8322 bits (120), Expect = 1.703e-8
Identity = 30/106 (28.30%), Postives = 53/106 (50.00%), Query Frame = 3 
Query:  108 QNGFSSFLWNIFLWSFVSNITAHLLAATVAIKNLWIHKHARIYAPLLYIFMSVLSIFTVNLFTSLSIASIYYTAEFPFNKWHAFIIGISQTMIMFVIAVTRITVTL 425
            Q   +   + +FLW+ VS++  H+ A  +A+  L  HK+ R +  +  + M ++   T  + TS +IA +Y  A      + A  +G  QT  + V++  RI  TL
Sbjct:    2 QRAAAEMWYGVFLWALVSSLFFHVPAGLLALFTLRHHKYGR-FMSVSILLMGIVGPITAGILTSAAIAGVYRAAGKEMIPFEALTLGTGQTFCVLVVSFLRILATL 106
[Close]
BLAST of SMED30000388 vs. Ensembl Human
Match: TMEM170A (transmembrane protein 170A [Source:HGNC Symbol;Acc:HGNC:29577])

HSP 1 Score: 50.447 bits (119), Expect = 2.642e-8
Identity = 29/98 (29.59%), Postives = 51/98 (52.04%), Query Frame = 3 
Query:  132 WNIFLWSFVSNITAHLLAATVAIKNLWIHKHARIYAPLLYIFMSVLSIFTVNLFTSLSIASIYYTAEFPFNKWHAFIIGISQTMIMFVIAVTRITVTL 425
            + +FLW+ VS++  H+ A  +A+  L  HK+ R +  +  + M ++   T  + TS +IA +Y  A      + A  +G  QT  + V++  RI  TL
Sbjct:    3 YGVFLWALVSSLFFHVPAGLLALFTLRHHKYGR-FMSVSILLMGIVGPITAGILTSAAIAGVYRAAGKEMIPFEALTLGTGQTFCVLVVSFLRILATL 99
[Close]
BLAST of SMED30000388 vs. Ensembl Human
Match: TMEM170A (transmembrane protein 170A [Source:HGNC Symbol;Acc:HGNC:29577])

HSP 1 Score: 50.8322 bits (120), Expect = 4.533e-8
Identity = 30/103 (29.13%), Postives = 52/103 (50.49%), Query Frame = 3 
Query:  117 FSSFLWNIFLWSFVSNITAHLLAATVAIKNLWIHKHARIYAPLLYIFMSVLSIFTVNLFTSLSIASIYYTAEFPFNKWHAFIIGISQTMIMFVIAVTRITVTL 425
            F    + +FLW+ VS++  H+ A  +A+  L  HK+ R +  +  + M ++   T  + TS +IA +Y  A      + A  +G  QT  + V++  RI  TL
Sbjct:   43 FPEMWYGVFLWALVSSLFFHVPAGLLALFTLRHHKYGR-FMSVSILLMGIVGPITAGILTSAAIAGVYRAAGKEMIPFEALTLGTGQTFCVLVVSFLRILATL 144
[Close]
BLAST of SMED30000388 vs. Ensembl Fly
Match: CG12341 (gene:FBgn0033550 transcript:FBtr0088251)

HSP 1 Score: 51.6026 bits (122), Expect = 6.624e-9
Identity = 34/105 (32.38%), Postives = 55/105 (52.38%), Query Frame = 3 
Query:  111 NGFSSFLWNIFLWSFVSNITAHLLAATVAIKNLWIHKHARIYAPLLYIFMSVLSIFTVNLFTSLSIASIYYTAEFPFNKWHAFIIGISQTMIMFVIAVTRITVTL 425
            N F    +++FLW+  S+I  H  AA VA   L  HK  R ++ +L + M  LS  +  + +S  IA ++  +  P +  +A I G+ QT++   +  TRI  TL
Sbjct:   25 NTFREMWYHVFLWALFSSIFIHTCAAVVAFFTLRKHKFGRFFS-ILILVMGFLSPASSGIISSAVIAFVHRASSLPMSPIYAMIWGLGQTIVSACLGFTRILATL 128
[Close]
BLAST of SMED30000388 vs. Ensembl Xenopus
Match: rnf182 (ring finger protein 182 [Source:Xenbase;Acc:XB-GENE-1009192])

HSP 1 Score: 53.5286 bits (127), Expect = 3.535e-9
Identity = 29/103 (28.16%), Postives = 54/103 (52.43%), Query Frame = 3 
Query:  117 FSSFLWNIFLWSFVSNITAHLLAATVAIKNLWIHKHARIYAPLLYIFMSVLSIFTVNLFTSLSIASIYYTAEFPFNKWHAFIIGISQTMIMFVIAVTRITVTL 425
            F    + +FLW+ VS++  H+ A  +A+  L  HK+ R +  +  + M +L   +  + TS +IA +Y  A      + A ++G+ QT  + +++  RI  TL
Sbjct:   41 FPEMWYGVFLWALVSSLFFHIPAGLLALFTLRHHKYGR-FMSVGILLMGILGPISAGILTSAAIAGVYKAAGKEMIPFEALVLGVGQTFCVLIVSFLRILATL 142
[Close]
BLAST of SMED30000388 vs. Ensembl Xenopus
Match: rnf182 (ring finger protein 182 [Source:Xenbase;Acc:XB-GENE-1009192])

HSP 1 Score: 53.5286 bits (127), Expect = 3.766e-9
Identity = 29/103 (28.16%), Postives = 54/103 (52.43%), Query Frame = 3 
Query:  117 FSSFLWNIFLWSFVSNITAHLLAATVAIKNLWIHKHARIYAPLLYIFMSVLSIFTVNLFTSLSIASIYYTAEFPFNKWHAFIIGISQTMIMFVIAVTRITVTL 425
            F    + +FLW+ VS++  H+ A  +A+  L  HK+ R +  +  + M +L   +  + TS +IA +Y  A      + A ++G+ QT  + +++  RI  TL
Sbjct:   44 FPEMWYGVFLWALVSSLFFHIPAGLLALFTLRHHKYGR-FMSVGILLMGILGPISAGILTSAAIAGVYKAAGKEMIPFEALVLGVGQTFCVLIVSFLRILATL 145
[Close]
BLAST of SMED30000388 vs. Ensembl Mouse
Match: Tmem170 (transmembrane protein 170 [Source:MGI Symbol;Acc:MGI:106426])

HSP 1 Score: 48.521 bits (114), Expect = 1.656e-7
Identity = 28/103 (27.18%), Postives = 52/103 (50.49%), Query Frame = 3 
Query:  117 FSSFLWNIFLWSFVSNITAHLLAATVAIKNLWIHKHARIYAPLLYIFMSVLSIFTVNLFTSLSIASIYYTAEFPFNKWHAFIIGISQTMIMFVIAVTRITVTL 425
            F    + +FLW+ +S++  H+ A  +A+  L  HK+ R +  +  + M ++   T  + TS +IA +Y  A      + A  +G  QT  + V++  R+  TL
Sbjct:   43 FPEMWYGVFLWALMSSVFFHVPAGLLALFTLRHHKYGR-FMSVSILLMGIVGPITAGILTSAAIAGVYRAAGKEMIPFEALTLGTGQTFCVVVVSFLRVLATL 144
[Close]
BLAST of SMED30000388 vs. UniProt/SwissProt
Match: sp|Q6DF87|T170A_XENLA (Transmembrane protein 170A OS=Xenopus laevis OX=8355 GN=tmem170a PE=2 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 1.012e-8
Identity = 30/103 (29.13%), Postives = 55/103 (53.40%), Query Frame = 3 
Query:  117 FSSFLWNIFLWSFVSNITAHLLAATVAIKNLWIHKHARIYAPLLYIFMSVLSIFTVNLFTSLSIASIYYTAEFPFNKWHAFIIGISQTMIMFVIAVTRITVTL 425
            F    + +FLW+ VS++  H+ A  +A+  L  HK+ R  +  +++ M VL   +  + TS +IA +Y  A      + A ++G+ QT  + +++  RI  TL
Sbjct:   41 FPEMWYGVFLWALVSSLFFHIPAGLLALFTLRHHKYGRFMSVGIFL-MGVLGPISAGILTSAAIAGVYKAAGKEMIPFEALVLGVGQTFCVLIVSFLRILATL 142
[Close]
BLAST of SMED30000388 vs. UniProt/SwissProt
Match: sp|Q5ZM31|T170A_CHICK (Transmembrane protein 170A OS=Gallus gallus OX=9031 GN=TMEM170A PE=2 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 4.593e-8
Identity = 32/103 (31.07%), Postives = 54/103 (52.43%), Query Frame = 3 
Query:  117 FSSFLWNIFLWSFVSNITAHLLAATVAIKNLWIHKHARIYAPLLYIFMSVLSIFTVNLFTSLSIASIYYTAEFPFNKWHAFIIGISQTMIMFVIAVTRITVTL 425
            F    + +FLW+ VS+++ H+ AA +A+  L  HK+ R  +  L + M ++   T  + TS +IA +Y  A      + A I  + QT  + V++  RI  TL
Sbjct:   37 FPEMWYGVFLWALVSSLSFHVPAALLALFTLRHHKYGRFMSVSL-LLMGIVGPITAGILTSAAIAGVYRAAGKKMIPFEALIFEVGQTFCVVVVSFLRILATL 138
[Close]
BLAST of SMED30000388 vs. UniProt/SwissProt
Match: sp|Q8WVE7|T170A_HUMAN (Transmembrane protein 170A OS=Homo sapiens OX=9606 GN=TMEM170A PE=1 SV=1)

HSP 1 Score: 50.8322 bits (120), Expect = 2.177e-7
Identity = 30/103 (29.13%), Postives = 52/103 (50.49%), Query Frame = 3 
Query:  117 FSSFLWNIFLWSFVSNITAHLLAATVAIKNLWIHKHARIYAPLLYIFMSVLSIFTVNLFTSLSIASIYYTAEFPFNKWHAFIIGISQTMIMFVIAVTRITVTL 425
            F    + +FLW+ VS++  H+ A  +A+  L  HK+ R +  +  + M ++   T  + TS +IA +Y  A      + A  +G  QT  + V++  RI  TL
Sbjct:   43 FPEMWYGVFLWALVSSLFFHVPAGLLALFTLRHHKYGR-FMSVSILLMGIVGPITAGILTSAAIAGVYRAAGKEMIPFEALTLGTGQTFCVLVVSFLRILATL 144
[Close]
BLAST of SMED30000388 vs. UniProt/SwissProt
Match: sp|Q9D342|T170A_MOUSE (Transmembrane protein 170A OS=Mus musculus OX=10090 GN=Tmem170a PE=2 SV=1)

HSP 1 Score: 48.521 bits (114), Expect = 1.158e-6
Identity = 28/103 (27.18%), Postives = 52/103 (50.49%), Query Frame = 3 
Query:  117 FSSFLWNIFLWSFVSNITAHLLAATVAIKNLWIHKHARIYAPLLYIFMSVLSIFTVNLFTSLSIASIYYTAEFPFNKWHAFIIGISQTMIMFVIAVTRITVTL 425
            F    + +FLW+ +S++  H+ A  +A+  L  HK+ R +  +  + M ++   T  + TS +IA +Y  A      + A  +G  QT  + V++  R+  TL
Sbjct:   43 FPEMWYGVFLWALMSSVFFHVPAGLLALFTLRHHKYGR-FMSVSILLMGIVGPITAGILTSAAIAGVYRAAGKEMIPFEALTLGTGQTFCVVVVSFLRVLATL 144
[Close]
BLAST of SMED30000388 vs. TrEMBL
Match: A0A0X3PQI6 (Transmembrane protein 170B OS=Schistocephalus solidus OX=70667 GN=T170B PE=4 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 1.329e-20
Identity = 40/98 (40.82%), Postives = 65/98 (66.33%), Query Frame = 3 
Query:  132 WNIFLWSFVSNITAHLLAATVAIKNLWIHKHARIYAPLLYIFMSVLSIFTVNLFTSLSIASIYYTAEFPFNKWHAFIIGISQTMIMFVIAVTRITVTL 425
            WNIFLW+   N   + +A  +A  NL +HK AR+Y PL+ + + ++S FTV L T L IA ++++   PF +WHA + G+ Q+++MFV +++R+  TL
Sbjct:   18 WNIFLWAASCNAVIYGIATVIAFVNLRVHKRARVYFPLVIVILGLISTFTVVLITCLVIAGVFHSMGLPFGEWHAVVCGVLQSLLMFVFSISRLPATL 115
[Close]
BLAST of SMED30000388 vs. TrEMBL
Match: A0A4S2LK89 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_006561 PE=4 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 2.222e-18
Identity = 45/99 (45.45%), Postives = 64/99 (64.65%), Query Frame = 3 
Query:  117 FSSFLWNIFLWSFVSNITAHLLAATVAIKNLWIHKHARIYAPLLYIFMSVLSIFTVNLFTSLSIASIYYTAEFPFNKWHAFIIGISQTMIMFVIAVTRI 413
            FSS  + IFLW+ VSNI+ H++A  VA  NL +HK  RI  PLL + M VL+  TV L +SL IA +Y      FN+ HA ++G+ QT + F+ +V+R+
Sbjct:   19 FSSIAFRIFLWAAVSNISVHVIALLVAFHNLKLHKTFRICVPLLILVMGVLNTVTVMLVSSLVIAGVYKATGISFNEVHAIVMGLLQTSLAFIFSVSRV 117
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BLAST of SMED30000388 vs. TrEMBL
Match: A0A1S8WKZ9 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=X801_09092 PE=4 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 4.785e-18
Identity = 45/99 (45.45%), Postives = 63/99 (63.64%), Query Frame = 3 
Query:  117 FSSFLWNIFLWSFVSNITAHLLAATVAIKNLWIHKHARIYAPLLYIFMSVLSIFTVNLFTSLSIASIYYTAEFPFNKWHAFIIGISQTMIMFVIAVTRI 413
            FSS  + IFLW+ VSNI+ H++A  VA  NL +HK  RI  PLL + M VL+  TV L +SL IA +Y      FN  HA ++G+ QT + F+ +V+R+
Sbjct:   19 FSSIAFRIFLWAAVSNISVHVIALLVAFHNLKLHKTFRICVPLLILGMGVLNTVTVMLVSSLVIAGVYKATGISFNDVHAIVMGLLQTSLAFIFSVSRV 117
[Close]
BLAST of SMED30000388 vs. TrEMBL
Match: A0A183T0T8 (Uncharacterized protein OS=Schistocephalus solidus OX=70667 PE=4 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 2.327e-17
Identity = 36/94 (38.30%), Postives = 61/94 (64.89%), Query Frame = 3 
Query:  144 LWSFVSNITAHLLAATVAIKNLWIHKHARIYAPLLYIFMSVLSIFTVNLFTSLSIASIYYTAEFPFNKWHAFIIGISQTMIMFVIAVTRITVTL 425
            LW+   N   + +A  +A  NL +HK AR+Y PL+ + + ++S FTV L T L IA ++++   PF +WHA + G+ Q+++MFV +++R+  TL
Sbjct:    1 LWAASCNAVIYGIATVIAFVNLRVHKRARVYFPLVIVILGLISTFTVVLITCLVIAGVFHSMGLPFGEWHAVVCGVLQSLLMFVFSISRLPATL 94
[Close]
BLAST of SMED30000388 vs. TrEMBL
Match: A0A3P7CE84 (Uncharacterized protein OS=Schistocephalus solidus OX=70667 GN=SSLN_LOCUS10084 PE=4 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 4.858e-17
Identity = 35/94 (37.23%), Postives = 61/94 (64.89%), Query Frame = 3 
Query:  144 LWSFVSNITAHLLAATVAIKNLWIHKHARIYAPLLYIFMSVLSIFTVNLFTSLSIASIYYTAEFPFNKWHAFIIGISQTMIMFVIAVTRITVTL 425
            +W+   N   + +A  +A  NL +HK AR+Y PL+ + + ++S FTV L T L IA ++++   PF +WHA + G+ Q+++MFV +++R+  TL
Sbjct:    1 MWAASCNAVIYGIATVIAFVNLRVHKRARVYFPLVIVILGLISTFTVVLITCLVIAGVFHSMGLPFGEWHAVVCGVLQSLLMFVFSISRLPATL 94
[Close]
BLAST of SMED30000388 vs. Ensembl Nematostella
Match: EDO49215 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RHE9])

HSP 1 Score: 55.8398 bits (133), Expect = 4.623e-11
Identity = 33/98 (33.67%), Postives = 53/98 (54.08%), Query Frame = 3 
Query:  132 WNIFLWSFVSNITAHLLAATVAIKNLWIHKHARIYAPLLYIFMSVLSIFTVNLFTSLSIASIYYTAEFPFNKWHAFIIGISQTMIMFVIAVTRITVTL 425
            + +FLW    ++  H +AA VA  +L  H   R + PL+ +F+  L   +    TS  IA++Y TA F    + AF+ G  Q+ I F+++ +RI  TL
Sbjct:    3 YQVFLWCLAGSVFIHCIAAFVACASLRKHSRGR-FLPLIILFVGFLYPVSGGAITSACIAAVYKTANFTMPVYVAFLWGAGQSFISFLLSYSRILPTL 99
[Close]
BLAST of SMED30000388 vs. Planmine SMEST
Match: SMESG000022724.1 (SMESG000022724.1)

HSP 1 Score: 212.231 bits (539), Expect = 3.966e-72
Identity = 108/108 (100.00%), Postives = 108/108 (100.00%), Query Frame = 3 
Query:  102 MSQNGFSSFLWNIFLWSFVSNITAHLLAATVAIKNLWIHKHARIYAPLLYIFMSVLSIFTVNLFTSLSIASIYYTAEFPFNKWHAFIIGISQTMIMFVIAVTRITVTL 425
            MSQNGFSSFLWNIFLWSFVSNITAHLLAATVAIKNLWIHKHARIYAPLLYIFMSVLSIFTVNLFTSLSIASIYYTAEFPFNKWHAFIIGISQTMIMFVIAVTRITVTL
Sbjct:    1 MSQNGFSSFLWNIFLWSFVSNITAHLLAATVAIKNLWIHKHARIYAPLLYIFMSVLSIFTVNLFTSLSIASIYYTAEFPFNKWHAFIIGISQTMIMFVIAVTRITVTL 108
The following BLAST results are available for this feature:
BLAST of SMED30000388 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
TMEM170A1.703e-828.30transmembrane protein 170A [Source:HGNC Symbol;Acc... [more]
TMEM170A2.642e-829.59transmembrane protein 170A [Source:HGNC Symbol;Acc... [more]
TMEM170A4.533e-829.13transmembrane protein 170A [Source:HGNC Symbol;Acc... [more]
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BLAST of SMED30000388 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000388 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
CG123416.624e-932.38gene:FBgn0033550 transcript:FBtr0088251[more]
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BLAST of SMED30000388 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000388 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rnf1823.535e-928.16ring finger protein 182 [Source:Xenbase;Acc:XB-GEN... [more]
rnf1823.766e-928.16ring finger protein 182 [Source:Xenbase;Acc:XB-GEN... [more]
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BLAST of SMED30000388 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Tmem1701.656e-727.18transmembrane protein 170 [Source:MGI Symbol;Acc:M... [more]
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BLAST of SMED30000388 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4
Match NameE-valueIdentityDescription
sp|Q6DF87|T170A_XENLA1.012e-829.13Transmembrane protein 170A OS=Xenopus laevis OX=83... [more]
sp|Q5ZM31|T170A_CHICK4.593e-831.07Transmembrane protein 170A OS=Gallus gallus OX=903... [more]
sp|Q8WVE7|T170A_HUMAN2.177e-729.13Transmembrane protein 170A OS=Homo sapiens OX=9606... [more]
sp|Q9D342|T170A_MOUSE1.158e-627.18Transmembrane protein 170A OS=Mus musculus OX=1009... [more]
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BLAST of SMED30000388 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0X3PQI61.329e-2040.82Transmembrane protein 170B OS=Schistocephalus soli... [more]
A0A4S2LK892.222e-1845.45Uncharacterized protein OS=Opisthorchis felineus O... [more]
A0A1S8WKZ94.785e-1845.45Uncharacterized protein OS=Opisthorchis viverrini ... [more]
A0A183T0T82.327e-1738.30Uncharacterized protein OS=Schistocephalus solidus... [more]
A0A3P7CE844.858e-1737.23Uncharacterized protein OS=Schistocephalus solidus... [more]
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BLAST of SMED30000388 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000388 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000388 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000388 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO492154.623e-1133.67Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30000388 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000388 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000022724.13.966e-72100.00SMESG000022724.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30000388 ID=SMED30000388|Name=SMED30000388|organism=Schmidtea mediterranea sexual|type=transcript|length=459bp
TTTAATAATTTGAAAAATAATTAAGATAATTTGTATTCGAAGTTGTTTAC
TAAATCCCAATAGTTTCTTTACCTAATCGAAAATTTAATTATTTTTTATT
AATGAGTCAGAATGGATTTTCAAGTTTTTTATGGAACATTTTTCTATGGT
CTTTTGTGTCAAATATTACTGCACATCTACTTGCAGCCACGGTAGCAATT
AAAAATCTGTGGATTCATAAGCATGCTAGAATTTATGCTCCGTTGTTGTA
TATTTTTATGAGTGTTTTAAGCATTTTCACAGTTAATTTATTCACAAGTC
TATCCATTGCCAGCATATATTACACTGCAGAATTTCCTTTTAATAAATGG
CATGCATTCATTATTGGTATATCTCAAACAATGATCATGTTTGTAATTGC
AGTTACTCGAATTACTGTCACCTTGTGATTTTTAATTGAAGATTTTAATT
TATAACCGC
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: INTERPRO
TermDefinition
IPR019334Transmembrane_pr_170
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019334Transmembrane protein 170PFAMPF10190Tmemb_170coord: 6..108
e-value: 6.5E-21
score: 74.7
IPR019334Transmembrane protein 170PANTHERPTHR22779UNCHARACTERIZEDcoord: 9..108
NoneNo IPR availableTMHMMTMhelixcoord: 85..107
NoneNo IPR availableTMHMMTMhelixcoord: 48..70
NoneNo IPR availableTMHMMTMhelixcoord: 13..35