Dmd-2 splice form 1

Overview
NameDmd-2 splice form 1 (AGL61627.1)
Smed IDSMED30000372
Length (bp)1145
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Dmd-2 splice form 1 (SMED30000372) t-SNE clustered cells

Violin plots show distribution of expression levels for Dmd-2 splice form 1 (SMED30000372) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Dmd-2 splice form 1 (SMED30000372) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Dmd-2 splice form 1 (SMED30000372) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30000372

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30000372h1SMcG0013880 dd_Smed_v4_31674_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Dmd-2 splice form 1 vs. Ensembl Human
Match: DMRTA2 (DMRT like family A2 [Source:HGNC Symbol;Acc:HGNC:13908])

HSP 1 Score: 94.3597 bits (233), Expect = 1.763e-20
Identity = 38/43 (88.37%), Postives = 39/43 (90.70%), Query Frame = 1
Query:  139 EKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            EKY RTPKCARCRNHG VS LKGHKRYCRWKDC+CAKC LIAE
Sbjct:   62 EKYPRTPKCARCRNHGVVSALKGHKRYCRWKDCLCAKCTLIAE 104          
BLAST of Dmd-2 splice form 1 vs. Ensembl Human
Match: DMRTA2 (DMRT like family A2 [Source:HGNC Symbol;Acc:HGNC:13908])

HSP 1 Score: 94.3597 bits (233), Expect = 1.763e-20
Identity = 38/43 (88.37%), Postives = 39/43 (90.70%), Query Frame = 1
Query:  139 EKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            EKY RTPKCARCRNHG VS LKGHKRYCRWKDC+CAKC LIAE
Sbjct:   62 EKYPRTPKCARCRNHGVVSALKGHKRYCRWKDCLCAKCTLIAE 104          
BLAST of Dmd-2 splice form 1 vs. Ensembl Human
Match: DMRTA1 (DMRT like family A1 [Source:HGNC Symbol;Acc:HGNC:13826])

HSP 1 Score: 87.0409 bits (214), Expect = 5.523e-18
Identity = 34/41 (82.93%), Postives = 36/41 (87.80%), Query Frame = 1
Query:  145 YQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            Y RTPKCARCRNHG VS LKGHKR+CRW+DC CAKC LIAE
Sbjct:   91 YPRTPKCARCRNHGVVSALKGHKRFCRWRDCACAKCTLIAE 131          
BLAST of Dmd-2 splice form 1 vs. Ensembl Human
Match: DMRT1 (doublesex and mab-3 related transcription factor 1 [Source:HGNC Symbol;Acc:HGNC:2934])

HSP 1 Score: 80.8777 bits (198), Expect = 3.608e-16
Identity = 32/43 (74.42%), Postives = 35/43 (81.40%), Query Frame = 1
Query:  139 EKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            +K  R PKCARCRNHGY SPLKGHKR+C W+DC C KC LIAE
Sbjct:   68 KKSPRLPKCARCRNHGYASPLKGHKRFCMWRDCQCKKCNLIAE 110          
BLAST of Dmd-2 splice form 1 vs. Ensembl Human
Match: DMRT2 (doublesex and mab-3 related transcription factor 2 [Source:HGNC Symbol;Acc:HGNC:2935])

HSP 1 Score: 75.485 bits (184), Expect = 3.480e-15
Identity = 31/42 (73.81%), Postives = 34/42 (80.95%), Query Frame = 1
Query:  142 KYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            K  RTPKCARCRNHG VS LKGHKR+CRW+DC CA C L+ E
Sbjct:  116 KLSRTPKCARCRNHGVVSCLKGHKRFCRWRDCQCANCLLVVE 157          
BLAST of Dmd-2 splice form 1 vs. Ensembl Celegans
Match: dmd-5 (DM (Doublesex/MAB-3) Domain family [Source:UniProtKB/TrEMBL;Acc:Q19291])

HSP 1 Score: 88.1965 bits (217), Expect = 1.181e-19
Identity = 35/43 (81.40%), Postives = 38/43 (88.37%), Query Frame = 1
Query:  139 EKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            E+ QRTPKCARCRNHG  S LKGHKRYC+WKDC+CAKC LIAE
Sbjct:   25 ERNQRTPKCARCRNHGTTSALKGHKRYCQWKDCMCAKCTLIAE 67          
BLAST of Dmd-2 splice form 1 vs. Ensembl Celegans
Match: dmd-4 (DM (Doublesex/MAB-3) Domain family [Source:UniProtKB/TrEMBL;Acc:Q18248])

HSP 1 Score: 77.7962 bits (190), Expect = 3.495e-16
Identity = 80/245 (32.65%), Postives = 113/245 (46.12%), Query Frame = 1
Query:  148 QRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAEXXXXXXXXXXXXXXXXXXXXXINDFVMYQNEEI-VYPSDSKKNIS-------DFQDEKTEPKPLTEQCGNSSTLHPQISESEIVAKSQCIWNSINFPITKKLLNVSSHMTSEKKFSVSNLLHSSPMISTYDPILYLKKSFPHISNQILQLVLEISHGNLWLATNLLIRQFFNFERLILPRLQSDFMKTSFLNQIGPTNIRSLITP 858
            +R PKCARCRNHG VS LKGHKR+C++K+C C KC LIAERQRVMAAQVAL+R+QA E+       +   + I   P     N +       D+ DE+ E KP T                               P++++L+ +      E           +  +S++ PI  L   F      +L+LVLE  HGN+  A    I  F N  R+        F   + ++ + P N+ + I P
Sbjct:   17 ERKPKCARCRNHGLVSWLKGHKRHCKYKECACEKCNLIAERQRVMAAQVALKRRQATEDAIALGLRVVAGQAIDRLPQGPVWNTAGGEDEDMDYLDEEIEEKPPT-------------------------------PVSEELVPLKRKKVEE-----------TYELSSFSPIELLMTLFCEHEKHVLELVLEACHGNVLQA----IEHFANVRRVKNINQMKLFAAATRMDHVFP-NMTNFILP 214          
BLAST of Dmd-2 splice form 1 vs. Ensembl Celegans
Match: dmd-10 (DM (Doublesex/MAB-3) Domain family [Source:UniProtKB/TrEMBL;Acc:G5EEJ1])

HSP 1 Score: 57.3806 bits (137), Expect = 4.222e-9
Identity = 21/40 (52.50%), Postives = 29/40 (72.50%), Query Frame = 1
Query:  148 QRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            +R P C +C  HG  S LKGHKR C +++C CAKCA+++E
Sbjct:   35 KRVPNCQKCGQHGRKSRLKGHKRSCPFRECPCAKCAVVSE 74          
BLAST of Dmd-2 splice form 1 vs. Ensembl Celegans
Match: dmd-3 (DM (Doublesex/MAB-3) Domain family [Source:UniProtKB/TrEMBL;Acc:Q9XWN9])

HSP 1 Score: 54.6842 bits (130), Expect = 7.259e-9
Identity = 21/44 (47.73%), Postives = 29/44 (65.91%), Query Frame = 1
Query:  136 FEKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
             ++ +R P C RC  H  V+ LKGHKR C ++DC CAKC ++ E
Sbjct:   14 VDRDERRPNCQRCAQHSVVNRLKGHKRACPFRDCFCAKCQVVVE 57          
BLAST of Dmd-2 splice form 1 vs. Ensembl Celegans
Match: dmd-3 (DM (Doublesex/MAB-3) Domain family [Source:UniProtKB/TrEMBL;Acc:Q9XWN9])

HSP 1 Score: 53.9138 bits (128), Expect = 5.214e-8
Identity = 21/40 (52.50%), Postives = 27/40 (67.50%), Query Frame = 1
Query:  148 QRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            +R P C RC  H  V+ LKGHKR C ++DC CAKC ++ E
Sbjct:  112 ERRPNCQRCAQHSVVNRLKGHKRACPFRDCFCAKCQVVVE 151          
BLAST of Dmd-2 splice form 1 vs. Ensembl Fly
Match: dmrt99B (gene:FBgn0039683 transcript:FBtr0085454)

HSP 1 Score: 97.8265 bits (242), Expect = 6.469e-22
Identity = 41/57 (71.93%), Postives = 43/57 (75.44%), Query Frame = 1
Query:   97 LKCLQPRQMFCRNFEKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            L  L P        E+YQRTPKCARCRNHG VS LKGHKRYCRW+DCVCAKC LIAE
Sbjct:   19 LGALPPAFFLRAASERYQRTPKCARCRNHGVVSALKGHKRYCRWRDCVCAKCTLIAE 75          
BLAST of Dmd-2 splice form 1 vs. Ensembl Fly
Match: dmrt11E (gene:FBgn0030477 transcript:FBtr0073768)

HSP 1 Score: 70.4774 bits (171), Expect = 4.239e-13
Identity = 26/48 (54.17%), Postives = 34/48 (70.83%), Query Frame = 1
Query:  124 FCRNFEKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
             C+   +  RTPKCARCRNHG +S +KGHKR CRW++C C  C L+ +
Sbjct:  109 ICQRERRLLRTPKCARCRNHGVISCVKGHKRLCRWRECCCPNCQLVVD 156          
BLAST of Dmd-2 splice form 1 vs. Ensembl Fly
Match: dmrt93B (gene:FBgn0038851 transcript:FBtr0084007)

HSP 1 Score: 66.2402 bits (160), Expect = 7.913e-12
Identity = 26/39 (66.67%), Postives = 31/39 (79.49%), Query Frame = 1
Query:  151 RTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            R PKCARCRNHG +S L+GHK+ C +K+C CAKC LI E
Sbjct:   39 RVPKCARCRNHGIISELRGHKKLCTYKNCKCAKCVLIFE 77          
BLAST of Dmd-2 splice form 1 vs. Ensembl Fly
Match: dsx (gene:FBgn0000504 transcript:FBtr0330074)

HSP 1 Score: 59.3066 bits (142), Expect = 2.966e-9
Identity = 23/37 (62.16%), Postives = 27/37 (72.97%), Query Frame = 1
Query:  157 PKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            P CARCRNHG    LKGHKRYC+++ C C KC L A+
Sbjct:   42 PNCARCRNHGLKITLKGHKRYCKFRYCTCEKCRLTAD 78          
BLAST of Dmd-2 splice form 1 vs. Ensembl Fly
Match: dsx (gene:FBgn0000504 transcript:FBtr0330073)

HSP 1 Score: 58.9214 bits (141), Expect = 3.337e-9
Identity = 23/37 (62.16%), Postives = 27/37 (72.97%), Query Frame = 1
Query:  157 PKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            P CARCRNHG    LKGHKRYC+++ C C KC L A+
Sbjct:   42 PNCARCRNHGLKITLKGHKRYCKFRYCTCEKCRLTAD 78          
BLAST of Dmd-2 splice form 1 vs. Ensembl Zebrafish
Match: dmrta2 (DMRT-like family A2 [Source:NCBI gene;Acc:474350])

HSP 1 Score: 95.5153 bits (236), Expect = 3.851e-21
Identity = 38/43 (88.37%), Postives = 39/43 (90.70%), Query Frame = 1
Query:  139 EKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            EKY RTPKCARCRNHG VS LKGHKRYCRWKDC+CAKC LIAE
Sbjct:   51 EKYPRTPKCARCRNHGVVSALKGHKRYCRWKDCMCAKCTLIAE 93          
BLAST of Dmd-2 splice form 1 vs. Ensembl Zebrafish
Match: dmrt1 (doublesex and mab-3 related transcription factor 1 [Source:NCBI gene;Acc:402923])

HSP 1 Score: 78.5666 bits (192), Expect = 5.261e-17
Identity = 31/45 (68.89%), Postives = 36/45 (80.00%), Query Frame = 1
Query:  133 NFEKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            +  K  R PKC+RCRNHG+VSPLKGHKR+C W+DC C KC LIAE
Sbjct:   12 SIRKPSRMPKCSRCRNHGFVSPLKGHKRFCNWRDCQCQKCRLIAE 56          
BLAST of Dmd-2 splice form 1 vs. Ensembl Zebrafish
Match: dmrt1 (doublesex and mab-3 related transcription factor 1 [Source:NCBI gene;Acc:402923])

HSP 1 Score: 78.5666 bits (192), Expect = 5.620e-17
Identity = 31/45 (68.89%), Postives = 36/45 (80.00%), Query Frame = 1
Query:  133 NFEKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            +  K  R PKC+RCRNHG+VSPLKGHKR+C W+DC C KC LIAE
Sbjct:   12 SIRKPSRMPKCSRCRNHGFVSPLKGHKRFCNWRDCQCQKCRLIAE 56          
BLAST of Dmd-2 splice form 1 vs. Ensembl Zebrafish
Match: dmrt1 (doublesex and mab-3 related transcription factor 1 [Source:NCBI gene;Acc:402923])

HSP 1 Score: 78.5666 bits (192), Expect = 1.150e-16
Identity = 31/45 (68.89%), Postives = 36/45 (80.00%), Query Frame = 1
Query:  133 NFEKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            +  K  R PKC+RCRNHG+VSPLKGHKR+C W+DC C KC LIAE
Sbjct:   12 SIRKPSRMPKCSRCRNHGFVSPLKGHKRFCNWRDCQCQKCRLIAE 56          
BLAST of Dmd-2 splice form 1 vs. Ensembl Zebrafish
Match: dmrt1 (doublesex and mab-3 related transcription factor 1 [Source:NCBI gene;Acc:402923])

HSP 1 Score: 78.5666 bits (192), Expect = 1.171e-16
Identity = 31/45 (68.89%), Postives = 36/45 (80.00%), Query Frame = 1
Query:  133 NFEKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            +  K  R PKC+RCRNHG+VSPLKGHKR+C W+DC C KC LIAE
Sbjct:   12 SIRKPSRMPKCSRCRNHGFVSPLKGHKRFCNWRDCQCQKCRLIAE 56          
BLAST of Dmd-2 splice form 1 vs. Ensembl Xenopus
Match: dynll1 (dynein light chain LC8-type 1 [Source:Xenbase;Acc:XB-GENE-854521])

HSP 1 Score: 99.7525 bits (247), Expect = 1.516e-22
Identity = 41/54 (75.93%), Postives = 44/54 (81.48%), Query Frame = 1
Query:  106 LQPRQMFCRNFEKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            L+  Q+  R  EKY RTPKCARCRNHG VS LKGHKRYCRWKDC+CAKC LIAE
Sbjct:   30 LRGPQLLLRAAEKYPRTPKCARCRNHGVVSALKGHKRYCRWKDCMCAKCTLIAE 83          
BLAST of Dmd-2 splice form 1 vs. Ensembl Xenopus
Match: dmrta1 (DMRT like family A1 [Source:NCBI gene;Acc:779901])

HSP 1 Score: 88.5817 bits (218), Expect = 1.083e-18
Identity = 93/237 (39.24%), Postives = 119/237 (50.21%), Query Frame = 1
Query:  145 YQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAEXXXXXXXXXXXXXXXXXXXXXINDF--VMYQNEEIVYPSD--------SKKNISDFQDEKTEPKPLTEQCGNSSTLH---PQISESEIV--------------AKSQCIWNS--------INFPITKKLLNVSSHMTSEKKFSVSNLLHSSPMIST--YDPILYLKKSFPHISNQILQLVLEISHGNLWLATNLLI 744
            Y RTPKCARCRNHG VS LKGHKR+CRW+DC CAKC LIAERQRVMAAQVALRRQQAQEE E+ D    +Y   E   P          S++   D   EKTE     E+   SS      PQ S S IV               K +CI +S         + P +    ++ S    E    +S      P++ +   DP+  L+K FP+     LQ +L+   G++  A   ++
Sbjct:   77 YPRTPKCARCRNHGVVSALKGHKRFCRWRDCSCAKCTLIAERQRVMAAQVALRRQQAQEECEVRDVQHFLYPGGERETPGTRAQTLQIISREENKDETSEKTE--QTYERIPTSSIFSHSLPQPSPSGIVNACHILKSAGPENSNKDECIQSSGSEERSEGADSPRSLSSCDLESGNECEWPKDLSASSSKIPVVPSRQRDPLEILRKVFPNQKQSSLQSILQYCKGDVVQAIEFVL 311          
BLAST of Dmd-2 splice form 1 vs. Ensembl Xenopus
Match: DMRT1 (doublesex and mab-3 related transcription factor 1 [Source:NCBI gene;Acc:100488257])

HSP 1 Score: 82.4185 bits (202), Expect = 6.008e-17
Identity = 32/43 (74.42%), Postives = 36/43 (83.72%), Query Frame = 1
Query:  139 EKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            +K  R PKCARCRNHGY SPLKGHKR+C W+DC C KC+LIAE
Sbjct:   24 KKSPRLPKCARCRNHGYASPLKGHKRFCMWRDCQCKKCSLIAE 66          
BLAST of Dmd-2 splice form 1 vs. Ensembl Xenopus
Match: kdm2b (lysine (K)-specific demethylase 2B [Source:Xenbase;Acc:XB-GENE-990506])

HSP 1 Score: 77.7962 bits (190), Expect = 5.367e-15
Identity = 31/42 (73.81%), Postives = 34/42 (80.95%), Query Frame = 1
Query:  142 KYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            K  RTPKCARCRNHG VS LKGHKR+CRW+DC CA C L+ E
Sbjct:   90 KLSRTPKCARCRNHGVVSCLKGHKRFCRWRDCQCANCLLVVE 131          
BLAST of Dmd-2 splice form 1 vs. Ensembl Xenopus
Match: st3gal4 (ST3 beta-galactoside alpha-2,3-sialyltransferase 4 [Source:Xenbase;Acc:XB-GENE-963280])

HSP 1 Score: 77.0258 bits (188), Expect = 7.528e-15
Identity = 32/40 (80.00%), Postives = 34/40 (85.00%), Query Frame = 1
Query:  148 QRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            QRTPKCARCRNHG +S LKGHKRYCR+KDC C KC LI E
Sbjct:   23 QRTPKCARCRNHGVLSWLKGHKRYCRFKDCTCEKCILIIE 62          
BLAST of Dmd-2 splice form 1 vs. Ensembl Mouse
Match: Dmrta2 (doublesex and mab-3 related transcription factor like family A2 [Source:MGI Symbol;Acc:MGI:2653629])

HSP 1 Score: 93.9745 bits (232), Expect = 1.799e-20
Identity = 38/43 (88.37%), Postives = 39/43 (90.70%), Query Frame = 1
Query:  139 EKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            EKY RTPKCARCRNHG VS LKGHKRYCRWKDC+CAKC LIAE
Sbjct:   61 EKYPRTPKCARCRNHGVVSALKGHKRYCRWKDCLCAKCTLIAE 103          
BLAST of Dmd-2 splice form 1 vs. Ensembl Mouse
Match: Dmrta1 (doublesex and mab-3 related transcription factor like family A1 [Source:MGI Symbol;Acc:MGI:2653627])

HSP 1 Score: 86.2705 bits (212), Expect = 5.608e-18
Identity = 34/41 (82.93%), Postives = 36/41 (87.80%), Query Frame = 1
Query:  145 YQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            Y RTPKCARCRNHG VS LKGHKR+CRW+DC CAKC LIAE
Sbjct:   80 YPRTPKCARCRNHGVVSALKGHKRFCRWRDCACAKCTLIAE 120          
BLAST of Dmd-2 splice form 1 vs. Ensembl Mouse
Match: Dmrt1 (doublesex and mab-3 related transcription factor 1 [Source:MGI Symbol;Acc:MGI:1354733])

HSP 1 Score: 81.2629 bits (199), Expect = 4.511e-17
Identity = 32/43 (74.42%), Postives = 36/43 (83.72%), Query Frame = 1
Query:  139 EKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            +K  R PKCARCRNHGY SPLKGHKR+C W+DC C KC+LIAE
Sbjct:   66 KKSPRLPKCARCRNHGYASPLKGHKRFCMWRDCQCKKCSLIAE 108          
BLAST of Dmd-2 splice form 1 vs. Ensembl Mouse
Match: Dmrt1 (doublesex and mab-3 related transcription factor 1 [Source:MGI Symbol;Acc:MGI:1354733])

HSP 1 Score: 82.0333 bits (201), Expect = 7.825e-17
Identity = 32/43 (74.42%), Postives = 36/43 (83.72%), Query Frame = 1
Query:  139 EKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            +K  R PKCARCRNHGY SPLKGHKR+C W+DC C KC+LIAE
Sbjct:   66 KKSPRLPKCARCRNHGYASPLKGHKRFCMWRDCQCKKCSLIAE 108          
BLAST of Dmd-2 splice form 1 vs. Ensembl Mouse
Match: Dmrt2 (doublesex and mab-3 related transcription factor 2 [Source:MGI Symbol;Acc:MGI:1330307])

HSP 1 Score: 78.1814 bits (191), Expect = 2.905e-15
Identity = 31/42 (73.81%), Postives = 34/42 (80.95%), Query Frame = 1
Query:  142 KYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            K  RTPKCARCRNHG VS LKGHKR+CRW+DC CA C L+ E
Sbjct:  116 KLSRTPKCARCRNHGVVSCLKGHKRFCRWRDCQCANCLLVVE 157          
BLAST of Dmd-2 splice form 1 vs. UniProt/SwissProt
Match: sp|A4QNP7|DMTA2_XENTR (Doublesex- and mab-3-related transcription factor A2 OS=Xenopus tropicalis OX=8364 GN=dmrta2 PE=2 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 8.030e-22
Identity = 41/54 (75.93%), Postives = 44/54 (81.48%), Query Frame = 1
Query:  106 LQPRQMFCRNFEKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            L+  Q+  R  EKY RTPKCARCRNHG VS LKGHKRYCRWKDC+CAKC LIAE
Sbjct:   30 LRGPQLLLRAAEKYPRTPKCARCRNHGVVSALKGHKRYCRWKDCMCAKCTLIAE 83          
BLAST of Dmd-2 splice form 1 vs. UniProt/SwissProt
Match: sp|Q2MJB4|DMTA2_XENLA (Doublesex- and mab-3-related transcription factor A2 OS=Xenopus laevis OX=8355 GN=dmrta2 PE=2 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 1.425e-21
Identity = 41/54 (75.93%), Postives = 44/54 (81.48%), Query Frame = 1
Query:  106 LQPRQMFCRNFEKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            L+  Q+  R  EKY RTPKCARCRNHG VS LKGHKRYCRWKDC+CAKC LIAE
Sbjct:   30 LRGPQLLLRAAEKYPRTPKCARCRNHGVVSALKGHKRYCRWKDCMCAKCTLIAE 83          
BLAST of Dmd-2 splice form 1 vs. UniProt/SwissProt
Match: sp|Q5UU75|DMTA2_DANRE (Doublesex- and mab-3-related transcription factor A2 OS=Danio rerio OX=7955 GN=dmrta2 PE=2 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 2.907e-20
Identity = 38/43 (88.37%), Postives = 39/43 (90.70%), Query Frame = 1
Query:  139 EKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            EKY RTPKCARCRNHG VS LKGHKRYCRWKDC+CAKC LIAE
Sbjct:   51 EKYPRTPKCARCRNHGVVSALKGHKRYCRWKDCMCAKCTLIAE 93          
BLAST of Dmd-2 splice form 1 vs. UniProt/SwissProt
Match: sp|A6QQ94|DMTA2_BOVIN (Doublesex- and mab-3-related transcription factor A2 OS=Bos taurus OX=9913 GN=DMRTA2 PE=2 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 7.860e-20
Identity = 38/43 (88.37%), Postives = 39/43 (90.70%), Query Frame = 1
Query:  139 EKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            EKY RTPKCARCRNHG VS LKGHKRYCRWKDC+CAKC LIAE
Sbjct:   61 EKYPRTPKCARCRNHGVVSALKGHKRYCRWKDCLCAKCTLIAE 103          
BLAST of Dmd-2 splice form 1 vs. UniProt/SwissProt
Match: sp|Q96SC8|DMTA2_HUMAN (Doublesex- and mab-3-related transcription factor A2 OS=Homo sapiens OX=9606 GN=DMRTA2 PE=2 SV=2)

HSP 1 Score: 94.3597 bits (233), Expect = 8.468e-20
Identity = 38/43 (88.37%), Postives = 39/43 (90.70%), Query Frame = 1
Query:  139 EKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            EKY RTPKCARCRNHG VS LKGHKRYCRWKDC+CAKC LIAE
Sbjct:   62 EKYPRTPKCARCRNHGVVSALKGHKRYCRWKDCLCAKCTLIAE 104          
BLAST of Dmd-2 splice form 1 vs. TrEMBL
Match: R4PV69 (Dmd-2 splice form 1 (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 223.787 bits (569), Expect = 2.358e-69
Identity = 127/129 (98.45%), Postives = 128/129 (99.22%), Query Frame = 1
Query:    4 LENSNSFNNTKINLSSVESIIDNEPKDFKSNLKCLQPRQMFCRNFEKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAEXXXXXXXXXXXXXXXXXXXXXINDFVMYQNEEIVYPSDSKK 390
            +ENSNSFNNTKINLSSVESIIDNEPKDFKSNLKCLQPRQMFCRNFEKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAERQRVMAAQVALRRQQAQEEQEINDFVMYQNEEIVYP DSKK
Sbjct:    1 MENSNSFNNTKINLSSVESIIDNEPKDFKSNLKCLQPRQMFCRNFEKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAERQRVMAAQVALRRQQAQEEQEINDFVMYQNEEIVYPPDSKK 129          
BLAST of Dmd-2 splice form 1 vs. TrEMBL
Match: R4PJK4 (Dmd-2 splice form 2 (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 162.54 bits (410), Expect = 2.756e-46
Identity = 75/76 (98.68%), Postives = 76/76 (100.00%), Query Frame = 1
Query:    4 LENSNSFNNTKINLSSVESIIDNEPKDFKSNLKCLQPRQMFCRNFEKYQRTPKCARCRNHGYVSPLKGHKRYCRWK 231
            +ENSNSFNNTKINLSSVESIIDNEPKDFKSNLKCLQPRQMFCRNFEKYQRTPKCARCRNHGYVSPLKGHKRYCRWK
Sbjct:    1 MENSNSFNNTKINLSSVESIIDNEPKDFKSNLKCLQPRQMFCRNFEKYQRTPKCARCRNHGYVSPLKGHKRYCRWK 76          
BLAST of Dmd-2 splice form 1 vs. TrEMBL
Match: K1RAP5 (Doublesex-and mab-3-related transcription factor A2 OS=Crassostrea gigas OX=29159 GN=CGI_10015952 PE=4 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 3.095e-22
Identity = 91/217 (41.94%), Postives = 125/217 (57.60%), Query Frame = 1
Query:  121 MFCRNFEKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAEXXXXXXXXXXXXXXXXXXXXXINDFVMYQN---------EEIVYPSDSKKNI-SDFQDEKTEPKPLTEQCGNSSTLHP--QISESEIVAKSQCIWNSINFPITKKLLNVSSHMTSE-KKFSVS----NLLHSSPMISTYDPILYLKKSFPHISNQILQLVLEISHGNL 720
            +F R  ++Y RTPKCARCRNHG VS LKGHKRYCRW+DCVCAKC LIAERQRVMAAQVALRRQQAQEE E  +  M            E I    D+KK + +   D +  P    ++  +S T  P  + S  ++  ++    +S + P + KL +  S    + + F  S     LL  +   S  +PI  L++ FPH+   +LQL+L+  +G++
Sbjct:   14 VFMRASDRYPRTPKCARCRNHGVVSALKGHKRYCRWRDCVCAKCTLIAERQRVMAAQVALRRQQAQEENEARELGMLYGPNGLLQLNPETITMFPDAKKVVDTSGSDREDGPATKRQKLDSSRTDSPVSRCSSEDMNERTHSPADSTSPPTSPKLADPPSPSDDKPEPFPKSPFEEGLLAGN---SKKNPIEMLQRIFPHMKRSVLQLILQGCNGDV 227          
BLAST of Dmd-2 splice form 1 vs. TrEMBL
Match: A7UD85 (Doublesex and mab-3 related transcription factor-like protein OS=Crassostrea gigas OX=29159 GN=DMl PE=2 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 3.285e-22
Identity = 91/217 (41.94%), Postives = 125/217 (57.60%), Query Frame = 1
Query:  121 MFCRNFEKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAEXXXXXXXXXXXXXXXXXXXXXINDFVMYQN---------EEIVYPSDSKKNI-SDFQDEKTEPKPLTEQCGNSSTLHP--QISESEIVAKSQCIWNSINFPITKKLLNVSSHMTSE-KKFSVS----NLLHSSPMISTYDPILYLKKSFPHISNQILQLVLEISHGNL 720
            +F R  ++Y RTPKCARCRNHG VS LKGHKRYCRW+DCVCAKC LIAERQRVMAAQVALRRQQAQEE E  +  M            E I    D+KK + +   D +  P    ++  +S T  P  + S  ++  ++    +S + P + KL +  S    + + F  S     LL  +   S  +PI  L++ FPH+   +LQL+L+  +G++
Sbjct:   14 VFMRASDRYPRTPKCARCRNHGVVSALKGHKRYCRWRDCVCAKCTLIAERQRVMAAQVALRRQQAQEENEARELGMLYGPNGLLQLNPETITMFPDAKKVVDTSGSDREDGPATKRQKLDSSRTDSPVSRCSSEDMNERTHSPADSTSPPTSPKLADPPSPSDDKPEPFPKSPFEEGLLAGN---SKKNPIEMLQRIFPHMKRSVLQLILQGCNGDV 227          
BLAST of Dmd-2 splice form 1 vs. TrEMBL
Match: A0A5N5SJ83 (Doublesex-and mab-3-related transcription factor A2 OS=Armadillidium nasatum OX=96803 GN=dmrta2_0 PE=4 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 4.200e-21
Identity = 41/52 (78.85%), Postives = 44/52 (84.62%), Query Frame = 1
Query:  112 PRQMFCRNFEKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            P  +F R  E+YQRTPKCARCRNHG VS LKGHKRYCRW+DCVCAKC LIAE
Sbjct:   17 PPALFLRASERYQRTPKCARCRNHGVVSALKGHKRYCRWRDCVCAKCTLIAE 68          
BLAST of Dmd-2 splice form 1 vs. Ensembl Cavefish
Match: dmrta2 (DMRT like family A2 [Source:NCBI gene;Acc:103023395])

HSP 1 Score: 95.1301 bits (235), Expect = 4.381e-21
Identity = 38/43 (88.37%), Postives = 39/43 (90.70%), Query Frame = 1
Query:  139 EKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            EKY RTPKCARCRNHG VS LKGHKRYCRWKDC+CAKC LIAE
Sbjct:   55 EKYPRTPKCARCRNHGVVSALKGHKRYCRWKDCMCAKCTLIAE 97          
BLAST of Dmd-2 splice form 1 vs. Ensembl Cavefish
Match: DMRTA1 (doublesex- and mab-3-related transcription factor A2 [Source:NCBI gene;Acc:103021351])

HSP 1 Score: 82.0333 bits (201), Expect = 5.863e-17
Identity = 31/41 (75.61%), Postives = 36/41 (87.80%), Query Frame = 1
Query:  145 YQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            + RTPKCARCRNHG +S LKGHKR CRW++C CA+CALIAE
Sbjct:   28 FPRTPKCARCRNHGVLSALKGHKRACRWRECACARCALIAE 68          
BLAST of Dmd-2 splice form 1 vs. Ensembl Cavefish
Match: DMRTA1 (doublesex- and mab-3-related transcription factor A2 [Source:NCBI gene;Acc:103021351])

HSP 1 Score: 82.0333 bits (201), Expect = 9.045e-17
Identity = 31/41 (75.61%), Postives = 36/41 (87.80%), Query Frame = 1
Query:  145 YQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            + RTPKCARCRNHG +S LKGHKR CRW++C CA+CALIAE
Sbjct:   42 FPRTPKCARCRNHGVLSALKGHKRACRWRECACARCALIAE 82          
BLAST of Dmd-2 splice form 1 vs. Ensembl Cavefish
Match: dmrt2a (doublesex- and mab-3-related transcription factor 2 [Source:NCBI gene;Acc:103040902])

HSP 1 Score: 79.7221 bits (195), Expect = 5.664e-16
Identity = 33/49 (67.35%), Postives = 37/49 (75.51%), Query Frame = 1
Query:  124 FCRNFEK-YQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
             C  F+K   RTPKCARCRNHG VS LKGHKR+CRW+DC CA C L+ E
Sbjct:    1 MCNTFQKKLSRTPKCARCRNHGVVSCLKGHKRFCRWRDCQCANCLLVVE 49          
BLAST of Dmd-2 splice form 1 vs. Ensembl Cavefish
Match: dmrt1 (doublesex- and mab-3-related transcription factor 1 [Source:NCBI gene;Acc:103041212])

HSP 1 Score: 77.411 bits (189), Expect = 1.053e-15
Identity = 29/39 (74.36%), Postives = 34/39 (87.18%), Query Frame = 1
Query:  151 RTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            R PKC+RCRNHG+VSPLKGHKR+C W+DC C KC LI+E
Sbjct:   30 RMPKCSRCRNHGFVSPLKGHKRFCNWRDCQCQKCKLISE 68          
BLAST of Dmd-2 splice form 1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004009.1 (pep scaffold:Pmarinus_7.0:GL483432:15080:15595:1 gene:ENSPMAG00000003668.1 transcript:ENSPMAT00000004009.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 95.5153 bits (236), Expect = 4.012e-24
Identity = 39/52 (75.00%), Postives = 43/52 (82.69%), Query Frame = 1
Query:  112 PRQMFCRNFEKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            P  +  R  +KYQRTPKCARCRNHG VS LKGHKRYCRW+DC+CAKC LIAE
Sbjct:   37 PHSLLLRAADKYQRTPKCARCRNHGVVSALKGHKRYCRWRDCLCAKCTLIAE 88          
BLAST of Dmd-2 splice form 1 vs. Ensembl Sea Lamprey
Match: dmrt2b (doublesex and mab-3 related transcription factor 2b [Source:ZFIN;Acc:ZDB-GENE-080813-1])

HSP 1 Score: 71.633 bits (174), Expect = 8.184e-15
Identity = 30/43 (69.77%), Postives = 34/43 (79.07%), Query Frame = 1
Query:  139 EKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
             +  RTPKCARCRNHG VS LKGHKR+CRW+DC CA C L+ E
Sbjct:   46 RRMARTPKCARCRNHG-VSCLKGHKRFCRWRDCQCANCLLVVE 87          
BLAST of Dmd-2 splice form 1 vs. Ensembl Nematostella
Match: EDO45848 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRL3])

HSP 1 Score: 79.7221 bits (195), Expect = 4.792e-19
Identity = 32/40 (80.00%), Postives = 34/40 (85.00%), Query Frame = 1
Query:  148 QRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            QRTPKCARCRNHG  S LKGHK YCRW+DC+C KC LIAE
Sbjct:    9 QRTPKCARCRNHGVCSSLKGHKYYCRWRDCLCTKCLLIAE 48          
BLAST of Dmd-2 splice form 1 vs. Ensembl Nematostella
Match: EDO41281 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4Z8])

HSP 1 Score: 78.5666 bits (192), Expect = 1.021e-18
Identity = 31/37 (83.78%), Postives = 33/37 (89.19%), Query Frame = 1
Query:  157 PKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            PKCARCRNHG VS LKGHKRYCRW+DC CA+C LIAE
Sbjct:    2 PKCARCRNHGVVSWLKGHKRYCRWRDCNCAQCTLIAE 38          
BLAST of Dmd-2 splice form 1 vs. Ensembl Nematostella
Match: EDO30270 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T198])

HSP 1 Score: 77.411 bits (189), Expect = 4.393e-18
Identity = 31/43 (72.09%), Postives = 35/43 (81.40%), Query Frame = 1
Query:  139 EKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            E   R+P+CARCRNHG VS LKGHKRYC+WKDC C KC+LI E
Sbjct:    9 ENLIRSPRCARCRNHGIVSYLKGHKRYCQWKDCTCTKCSLIIE 51          
BLAST of Dmd-2 splice form 1 vs. Ensembl Nematostella
Match: EDO37386 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SFT8])

HSP 1 Score: 76.6406 bits (187), Expect = 4.848e-18
Identity = 30/39 (76.92%), Postives = 33/39 (84.62%), Query Frame = 1
Query:  151 RTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            R PKCARCRNHG +S LKGHK YCRW+DC C+KC LIAE
Sbjct:    1 RIPKCARCRNHGALSWLKGHKHYCRWRDCTCSKCLLIAE 39          
BLAST of Dmd-2 splice form 1 vs. Ensembl Nematostella
Match: EDO35744 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SKL0])

HSP 1 Score: 74.7146 bits (182), Expect = 2.900e-17
Identity = 29/39 (74.36%), Postives = 32/39 (82.05%), Query Frame = 1
Query:  151 RTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            R PKCARCR+HG VS LKGHK YCRW+DC C+KC LI E
Sbjct:    1 RVPKCARCRSHGTVSWLKGHKHYCRWRDCTCSKCQLITE 39          
BLAST of Dmd-2 splice form 1 vs. Ensembl Medaka
Match: dmrta2 (DMRT like family A2 [Source:NCBI gene;Acc:111947399])

HSP 1 Score: 93.5893 bits (231), Expect = 1.242e-20
Identity = 37/43 (86.05%), Postives = 39/43 (90.70%), Query Frame = 1
Query:  139 EKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            +KY RTPKCARCRNHG VS LKGHKRYCRWKDC+CAKC LIAE
Sbjct:   49 DKYPRTPKCARCRNHGVVSALKGHKRYCRWKDCMCAKCTLIAE 91          
BLAST of Dmd-2 splice form 1 vs. Ensembl Medaka
Match: dmrta2 (DMRT like family A2 [Source:NCBI gene;Acc:111947399])

HSP 1 Score: 93.5893 bits (231), Expect = 1.242e-20
Identity = 37/43 (86.05%), Postives = 39/43 (90.70%), Query Frame = 1
Query:  139 EKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            +KY RTPKCARCRNHG VS LKGHKRYCRWKDC+CAKC LIAE
Sbjct:   49 DKYPRTPKCARCRNHGVVSALKGHKRYCRWKDCMCAKCTLIAE 91          
BLAST of Dmd-2 splice form 1 vs. Ensembl Medaka
Match: DMRTA1 (DMRT like family A1 [Source:NCBI gene;Acc:101165100])

HSP 1 Score: 90.1225 bits (222), Expect = 1.667e-19
Identity = 40/59 (67.80%), Postives = 45/59 (76.27%), Query Frame = 1
Query:  106 LQPRQMFCRN----FEK-YQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            L+P  +F R      E+ Y RTPKCARCRNHG VS LKGHKR+CRW+DCVCAKC LIAE
Sbjct:   28 LRPPSLFLRACNPALERGYPRTPKCARCRNHGVVSALKGHKRFCRWRDCVCAKCTLIAE 86          
BLAST of Dmd-2 splice form 1 vs. Ensembl Medaka
Match: dmy (Oryzias latipes doublesex- and mab-3-related transcription factor 1 (dmy), mRNA. [Source:RefSeq mRNA;Acc:NM_001104680])

HSP 1 Score: 82.8037 bits (203), Expect = 1.031e-18
Identity = 33/43 (76.74%), Postives = 38/43 (88.37%), Query Frame = 1
Query:  139 EKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            ++  R PKC+RCRNHG+VSPLKGHKR+CRWKDC CAKC LIAE
Sbjct:   19 QRSPRMPKCSRCRNHGFVSPLKGHKRFCRWKDCRCAKCKLIAE 61          
BLAST of Dmd-2 splice form 1 vs. Ensembl Medaka
Match: dmy (Oryzias latipes doublesex- and mab-3-related transcription factor 1 (dmy), mRNA. [Source:RefSeq mRNA;Acc:NM_001104680])

HSP 1 Score: 85.1149 bits (209), Expect = 2.469e-18
Identity = 33/43 (76.74%), Postives = 38/43 (88.37%), Query Frame = 1
Query:  139 EKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            ++  R PKC+RCRNHG+VSPLKGHKR+CRWKDC CAKC LIAE
Sbjct:   19 QRSPRMPKCSRCRNHGFVSPLKGHKRFCRWKDCRCAKCKLIAE 61          
BLAST of Dmd-2 splice form 1 vs. Planmine SMEST
Match: SMESG000075920.1 (SMESG000075920.1)

HSP 1 Score: 648.277 bits (1671), Expect = 0.000e+0
Identity = 337/341 (98.83%), Postives = 339/341 (99.41%), Query Frame = 1
Query:    4 LENSNSFNNTKINLSSVESIIDNEPKDFKSNLKCLQPRQMFCRNFEKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAEXXXXXXXXXXXXXXXXXXXXXINDFVMYQNEEIVYPSDSKKNISDFQDEKTEPKPLTEQCGNSSTLHPQISESEIVAKSQCIWNSINFPITKKLLNVSSHMTSEKKFSVSNLLHSSPMISTYDPILYLKKSFPHISNQILQLVLEISHGNLWLATNLLIRQFFNFERLILPRLQSDFMKTSFLNQIGPTNIRSLITPNDFMEPNSLCEEKSNCQELYDQYGIFSNGNFSQSFGPKLNPYNKLETNTNHEFKCD 1026
            +ENSNSFNNTKINLSSVESIIDNEPKDFKSNLKCLQPRQMFCRNFEKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAERQRVMAAQVALRRQQAQEEQEINDFVMYQNEEIVYP DSKKNISDFQDEKTEPKPLTEQCGNSSTLHPQISESEIVAKSQCIWNSINFPITKKLLNVSSHMTSEKKFSVSNLLHSSPMISTYDPILYLKKSFPHISNQILQLVLEISHGNLWLATNLLIRQFFNFERLILPRLQSDFMKTSFLNQIGPTNIRSLITPNDFME NSLCEEKSNCQELYDQYGIFSNGNFSQ+FGPKLNPYNKLETNTNHEFKCD
Sbjct:    1 MENSNSFNNTKINLSSVESIIDNEPKDFKSNLKCLQPRQMFCRNFEKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAERQRVMAAQVALRRQQAQEEQEINDFVMYQNEEIVYPPDSKKNISDFQDEKTEPKPLTEQCGNSSTLHPQISESEIVAKSQCIWNSINFPITKKLLNVSSHMTSEKKFSVSNLLHSSPMISTYDPILYLKKSFPHISNQILQLVLEISHGNLWLATNLLIRQFFNFERLILPRLQSDFMKTSFLNQIGPTNIRSLITPNDFMEQNSLCEEKSNCQELYDQYGIFSNGNFSQNFGPKLNPYNKLETNTNHEFKCD 341          
BLAST of Dmd-2 splice form 1 vs. Planmine SMEST
Match: SMESG000006521.1 (SMESG000006521.1)

HSP 1 Score: 79.7221 bits (195), Expect = 5.110e-16
Identity = 40/71 (56.34%), Postives = 48/71 (67.61%), Query Frame = 1
Query:   55 ESIIDNEPKDFKSNLKCLQPRQMFCRNFEKYQRTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAE 267
            +S+ D  P+  ++ LK +QP        EK  R PKCARCRNHG VS LKGHKR+C +KDC CAKC LIAE
Sbjct:   10 KSLSDQTPEFSRNFLKLMQPPLYPIT--EKGARKPKCARCRNHGMVSWLKGHKRHCHYKDCRCAKCNLIAE 78          
The following BLAST results are available for this feature:
BLAST of Dmd-2 splice form 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
DMRTA21.763e-2088.37DMRT like family A2 [Source:HGNC Symbol;Acc:HGNC:1... [more]
DMRTA21.763e-2088.37DMRT like family A2 [Source:HGNC Symbol;Acc:HGNC:1... [more]
DMRTA15.523e-1882.93DMRT like family A1 [Source:HGNC Symbol;Acc:HGNC:1... [more]
DMRT13.608e-1674.42doublesex and mab-3 related transcription factor 1... [more]
DMRT23.480e-1573.81doublesex and mab-3 related transcription factor 2... [more]
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BLAST of Dmd-2 splice form 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
dmd-51.181e-1981.40DM (Doublesex/MAB-3) Domain family [Source:UniPro... [more]
dmd-43.495e-1632.65DM (Doublesex/MAB-3) Domain family [Source:UniPro... [more]
dmd-104.222e-952.50DM (Doublesex/MAB-3) Domain family [Source:UniPro... [more]
dmd-37.259e-947.73DM (Doublesex/MAB-3) Domain family [Source:UniPro... [more]
dmd-35.214e-852.50DM (Doublesex/MAB-3) Domain family [Source:UniPro... [more]
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BLAST of Dmd-2 splice form 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
dmrt99B6.469e-2271.93gene:FBgn0039683 transcript:FBtr0085454[more]
dmrt11E4.239e-1354.17gene:FBgn0030477 transcript:FBtr0073768[more]
dmrt93B7.913e-1266.67gene:FBgn0038851 transcript:FBtr0084007[more]
dsx2.966e-962.16gene:FBgn0000504 transcript:FBtr0330074[more]
dsx3.337e-962.16gene:FBgn0000504 transcript:FBtr0330073[more]
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BLAST of Dmd-2 splice form 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
dmrta23.851e-2188.37DMRT-like family A2 [Source:NCBI gene;Acc:474350][more]
dmrt15.261e-1768.89doublesex and mab-3 related transcription factor 1... [more]
dmrt15.620e-1768.89doublesex and mab-3 related transcription factor 1... [more]
dmrt11.150e-1668.89doublesex and mab-3 related transcription factor 1... [more]
dmrt11.171e-1668.89doublesex and mab-3 related transcription factor 1... [more]
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BLAST of Dmd-2 splice form 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
dynll11.516e-2275.93dynein light chain LC8-type 1 [Source:Xenbase;Acc:... [more]
dmrta11.083e-1839.24DMRT like family A1 [Source:NCBI gene;Acc:779901][more]
DMRT16.008e-1774.42doublesex and mab-3 related transcription factor 1... [more]
kdm2b5.367e-1573.81lysine (K)-specific demethylase 2B [Source:Xenbase... [more]
st3gal47.528e-1580.00ST3 beta-galactoside alpha-2,3-sialyltransferase 4... [more]
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BLAST of Dmd-2 splice form 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Dmrta21.799e-2088.37doublesex and mab-3 related transcription factor l... [more]
Dmrta15.608e-1882.93doublesex and mab-3 related transcription factor l... [more]
Dmrt14.511e-1774.42doublesex and mab-3 related transcription factor 1... [more]
Dmrt17.825e-1774.42doublesex and mab-3 related transcription factor 1... [more]
Dmrt22.905e-1573.81doublesex and mab-3 related transcription factor 2... [more]
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BLAST of Dmd-2 splice form 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|A4QNP7|DMTA2_XENTR8.030e-2275.93Doublesex- and mab-3-related transcription factor ... [more]
sp|Q2MJB4|DMTA2_XENLA1.425e-2175.93Doublesex- and mab-3-related transcription factor ... [more]
sp|Q5UU75|DMTA2_DANRE2.907e-2088.37Doublesex- and mab-3-related transcription factor ... [more]
sp|A6QQ94|DMTA2_BOVIN7.860e-2088.37Doublesex- and mab-3-related transcription factor ... [more]
sp|Q96SC8|DMTA2_HUMAN8.468e-2088.37Doublesex- and mab-3-related transcription factor ... [more]
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BLAST of Dmd-2 splice form 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
R4PV692.358e-6998.45Dmd-2 splice form 1 (Fragment) OS=Schmidtea medite... [more]
R4PJK42.756e-4698.68Dmd-2 splice form 2 (Fragment) OS=Schmidtea medite... [more]
K1RAP53.095e-2241.94Doublesex-and mab-3-related transcription factor A... [more]
A7UD853.285e-2241.94Doublesex and mab-3 related transcription factor-l... [more]
A0A5N5SJ834.200e-2178.85Doublesex-and mab-3-related transcription factor A... [more]
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BLAST of Dmd-2 splice form 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
dmrta24.381e-2188.37DMRT like family A2 [Source:NCBI gene;Acc:10302339... [more]
DMRTA15.863e-1775.61doublesex- and mab-3-related transcription factor ... [more]
DMRTA19.045e-1775.61doublesex- and mab-3-related transcription factor ... [more]
dmrt2a5.664e-1667.35doublesex- and mab-3-related transcription factor ... [more]
dmrt11.053e-1574.36doublesex- and mab-3-related transcription factor ... [more]
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BLAST of Dmd-2 splice form 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSPMAT00000004009.14.012e-2475.00pep scaffold:Pmarinus_7.0:GL483432:15080:15595:1 g... [more]
dmrt2b8.184e-1569.77doublesex and mab-3 related transcription factor 2... [more]
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BLAST of Dmd-2 splice form 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dmd-2 splice form 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO458484.792e-1980.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO412811.021e-1883.78Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO302704.393e-1872.09Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO373864.848e-1876.92Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO357442.900e-1774.36Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Dmd-2 splice form 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
dmrta21.242e-2086.05DMRT like family A2 [Source:NCBI gene;Acc:11194739... [more]
dmrta21.242e-2086.05DMRT like family A2 [Source:NCBI gene;Acc:11194739... [more]
DMRTA11.667e-1967.80DMRT like family A1 [Source:NCBI gene;Acc:10116510... [more]
dmy1.031e-1876.74Oryzias latipes doublesex- and mab-3-related trans... [more]
dmy2.469e-1876.74Oryzias latipes doublesex- and mab-3-related trans... [more]
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BLAST of Dmd-2 splice form 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000075920.10.000e+098.83SMESG000075920.1[more]
SMESG000006521.15.110e-1656.34SMESG000006521.1[more]
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Cross References
External references for this transcript
DatabaseAccession
SmedGD GBrowseSMED30000372
GenBankAGL61627.1
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30000372 ID=SMED30000372|Name=Dmd-2 splice form 1|organism=Schmidtea mediterranea sexual|type=transcript|length=1145bp
GATCTGGAAAATTCTAATAGCTTTAATAATACAAAAATTAATTTAAGTAG
TGTGGAATCTATTATAGATAATGAACCAAAAGATTTTAAATCAAATCTTA
AATGTTTGCAGCCTAGGCAAATGTTTTGTCGAAATTTTGAAAAATACCAA
CGAACTCCTAAATGTGCACGTTGCCGCAATCATGGATATGTTTCACCTTT
AAAAGGTCACAAGCGATACTGTAGATGGAAAGATTGTGTCTGTGCAAAAT
GTGCTCTAATAGCGGAACGACAAAGAGTGATGGCAGCTCAGGTTGCATTG
CGGAGACAACAAGCACAAGAGGAGCAGGAAATTAATGATTTTGTCATGTA
TCAAAATGAAGAAATTGTTTACCCGTCAGATTCCAAGAAAAATATTTCTG
ATTTTCAAGATGAAAAAACCGAACCCAAACCTTTAACGGAACAATGTGGA
AATAGCTCTACTCTTCATCCTCAAATATCTGAATCTGAAATCGTTGCCAA
ATCTCAATGTATTTGGAATTCTATCAACTTCCCAATTACGAAAAAATTAC
TAAACGTGTCATCTCACATGACTTCCGAAAAGAAATTCAGCGTTTCCAAC
TTATTGCATTCAAGTCCTATGATCAGTACTTATGATCCGATTTTATATTT
AAAGAAATCTTTTCCACATATTTCCAATCAAATATTACAGTTGGTTCTTG
AAATAAGTCATGGGAATTTGTGGTTAGCTACGAATCTTTTGATTCGGCAA
TTTTTTAATTTTGAAAGACTCATCCTTCCAAGATTACAAAGCGACTTCAT
GAAAACCAGTTTTTTGAATCAAATCGGTCCCACTAACATCCGATCTTTGA
TTACGCCAAATGATTTCATGGAACCAAATTCGCTATGTGAGGAAAAATCA
AATTGTCAAGAGCTTTATGACCAATATGGAATCTTTTCAAATGGTAATTT
TTCCCAAAGTTTTGGTCCCAAACTAAATCCGTACAATAAACTCGAGACCA
ACACAAATCATGAATTTAAATGCGATTAAACTATGACTTTTAATCTGTGT
TTTCTTTCATTATTTTTTACATTTTTTCTCGATAAATGCTTTGTAATTAA
AATTTGTGTTCATTGTAGAATCCTAAAGTTATTGTTACCTTTTAC
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protein sequence of SMED30000372-orf-1

>SMED30000372-orf-1 ID=SMED30000372-orf-1|Name=SMED30000372-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=343bp
DLENSNSFNNTKINLSSVESIIDNEPKDFKSNLKCLQPRQMFCRNFEKYQ
RTPKCARCRNHGYVSPLKGHKRYCRWKDCVCAKCALIAERQRVMAAQVAL
RRQQAQEEQEINDFVMYQNEEIVYPSDSKKNISDFQDEKTEPKPLTEQCG
NSSTLHPQISESEIVAKSQCIWNSINFPITKKLLNVSSHMTSEKKFSVSN
LLHSSPMISTYDPILYLKKSFPHISNQILQLVLEISHGNLWLATNLLIRQ
FFNFERLILPRLQSDFMKTSFLNQIGPTNIRSLITPNDFMEPNSLCEEKS
NCQELYDQYGIFSNGNFSQSFGPKLNPYNKLETNTNHEFKCD*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: INTERPRO
TermDefinition
IPR036407DM_DNA-bd_sf
IPR026607DMRT/dsx/mab-3
IPR001275DM_DNA-bd
Vocabulary: molecular function
TermDefinition
GO:0043565sequence-specific DNA binding
GO:0003677DNA binding
GO:0003700transcription factor activity, sequence-specific DNA binding
GO:0046872metal ion binding
Vocabulary: biological process
TermDefinition
GO:0006355regulation of transcription, DNA-templated
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001275DM DNA-binding domainSMARTSM00301DM_3coord: 49..102
e-value: 1.9E-27
score: 107.2
IPR001275DM DNA-binding domainPFAMPF00751DMcoord: 49..95
e-value: 5.5E-23
score: 80.6
IPR001275DM DNA-binding domainPROSITEPS40000DM_1coord: 53..82
IPR001275DM DNA-binding domainPROSITEPS50809DM_2coord: 53..100
score: 14.391
IPR036407DM DNA-binding domain superfamilyGENE3DG3DSA:4.10.1040.10coord: 46..95
e-value: 2.4E-23
score: 83.6
IPR036407DM DNA-binding domain superfamilySUPERFAMILYSSF82927Cysteine-rich DNA binding domain, (DM domain)coord: 47..95
IPR026607DMRT/protein doublesex/protein male abnormal 3PANTHERPTHR12322DOUBLESEX AND MAB-3 RELATED TRANSCRIPTION FACTOR DMRTcoord: 45..198
NoneNo IPR availableCDDcd14279CUEcoord: 210..245
e-value: 8.44769E-4
score: 34.3662