Rapunzel

Overview
NameRapunzel
Smed IDSMED30000350
Length (bp)1477
Neoblast Clusters

Zeng et. al., 2018



 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30000350

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30000350h1SMcG0015361 SmedASXL_004259SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pigment cellSMED30000350h1SMcG0015361 SmedASXL_004259SmedAsxl_ww_GCZZ01PMID:29158443
He et al., 2017		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30000350h1SMcG0015361 dd_Smed_v6_5881_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30000350h1SMcG0015361 OX_Smed_1.0.17127ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Rapunzel vs. Ensembl Zebrafish
Match: rpz4 (rapunzel 4 [Source:ZFIN;Acc:ZDB-GENE-050417-472])

HSP 1 Score: 56.225 bits (134), Expect = 4.937e-8
Identity = 68/299 (22.74%), Postives = 124/299 (41.47%), Query Frame = 3 
Query:  297 FNKVGKLMADITDETFNV-SSSTIFTAFLLGELHATETESFKKASLLLKTRTSRWKEDFNEVRYSIDWAVVQINFEDLENRILTVQRLSTEFMSIDDNEKEFSAKQFLHSYEANYKATGD----LLYSVMININQSFSG-NLFRASQKFTQFDRKKVQTFMVELAKLLVQAVNIEANFANLKYENDENVKFLNSMWRERFKTLKATMEAVDLDTKEQFRNQYQMDLDEYLMTRESLTNEQFNQVLYNYLSTKYNWRDWFVVVYAPISGF----KDHTVSTCQGTYRFRT--NGRNIIVS 1157
            F +  +++A I  + F V S +       L  + + E E  K+    ++ R     E+  ++   I  + V   +  +E  +    R   + ++     +E   K FL  +    +  GD     LYS +   N  FSG ++   +  + Q  R+ V+ +   L KL    +      A LK  +DE  K L   W E+ K +++ M  V  D    F  Q ++D    +  +   +N+Q   +L  +L  KY+W  W V ++   +G     KD    T +  ++  T  +  N++VS
Sbjct:   23 FEQGAEVVASIAGDLFPVFSIAAPIVRLALDNVESKEAEYMKEQFQKVRDRLEVVSEEVQQINQEIKKSGVDATYFTVEENLTNQFRKFMDVLNAKPKFREVRKKTFLDHFN---RTGGDKNLHTLYSAVTGDN--FSGESVLEITLNYEQKSRRAVEEYCATLKKLFCVGLIALLGHAALKG-DDEEEKLLQE-WSEKMKVVQSKMIVVIEDCINSFPTQSELDAKRIVRDQSDKSNQQLADMLVEHLKKKYDWVCWSVRIFNSPTGLFTNKKDFQGLTGKSRFQVPTSDDKLNVVVS 314
[Close]
BLAST of Rapunzel vs. Ensembl Zebrafish
Match: rpz5 (rapunzel 5 [Source:ZFIN;Acc:ZDB-GENE-030131-4678])

HSP 1 Score: 48.9062 bits (115), Expect = 8.306e-6
Identity = 31/110 (28.18%), Postives = 51/110 (46.36%), Query Frame = 3 
Query:  738 RKKVQTFMVELAKLLVQAVNIEANFANLKYENDENVKFLNSMWRERFKTLKATMEAVDLDTKEQFRNQYQMDLDEYLMTRESLTNEQFNQVLYNYLSTKYNWRDWFVVVY 1067
            RK V+ F   L KL V  +      A LK       + +   W++R + ++  M+A   D  E F  Q + D++  L+ R++  N +F   + + L  KY W  W V V+
Sbjct:  170 RKPVEEFCARLKKLFVMGIIAVMGHAALK--EGAVGEAMVKKWQDRMEDVEKRMKAAVDDCIENFPLQAKTDVERQLLERQANVNPEFTGFILDILEKKYYWVSWSVRVF 277
[Close]
BLAST of Rapunzel vs. TrEMBL
Match: A0A0A7NXM5 (Rapunzel (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 351.673 bits (901), Expect = 1.523e-114
Identity = 168/366 (45.90%), Postives = 238/366 (65.03%), Query Frame = 3 
Query:  249 GIQAITQMEKFYGEKDFNKVGKLMADITDETFNVSSSTIFTAFLLGELHATETESFKKASLLLKTRTSRWKEDFNEVRYSIDWAVVQINFEDLENRILTVQRLSTEFMSIDDNEKEFSAKQFLHSYEANYKATGDLLYSVMININQSFSGNLFRASQKFTQFDRKKVQTFMVELAKLLVQAVNIEANFANLKYENDENVKFLNSMWRERFKTLKATMEAVDLDTKEQFRNQYQMDLDEYLMTRESLTNEQFNQVLYNYLSTKYNWRDWFVVVYAPISGFKDHTVSTCQGTYRFRTNGRNIIVSSVDKEDGISAGSHIERNKQIIGSYSDWKSDKEYSRGWVSFMDKLLPNCNDADTRLSVAWGSNV 1346
            G+Q + Q+ KF  EK+F K    ++ +    F+  S+ +   F+  E+ + E    KK    L  R  +W  +F  ++  IDW+ VQINF D+EN I+T+ ++ +    +    +E  A  F+++YE NYKA GD+L+  M+NI Q +S NL RA+Q + + DR +VQ FMV + KL+ QAV IEA +  LKY N+E  KF  S+W ++   LK  +E +D DTK ++  QYQ DLD  L T  S +NE+F  + Y++ +TKYNWR+WFV+VY  +SGFKDH V TCQGT+RFRT+GRNIIVSSVD+ +G +A +HI RNKQIIG+Y+      E+S+ WV +  + LP C+DADTR SVAWGSNV
Sbjct:    3 GVQGVVQLFKFLEEKNFFKTLTQISSVAGPIFSAISAILTVVFMFIEIESPEMALMKKEFANLNNRIDKWGVEFQIIKRKIDWSAVQINFADIENNIITLHKMLSTLPEVPIANRETQAALFINTYEINYKAAGDILHRAMLNIEQIYSVNLLRAAQLYMENDRNQVQEFMVGMMKLITQAVQIEAAYVGLKYNNEEASKFTQSLWEKKLAVLKDKLEQIDTDTKNKYNEQYQKDLDNLLATHYSQSNERFVTIAYDFFATKYNWREWFVLVYKELSGFKDHAVVTCQGTHRFRTHGRNIIVSSVDQSEGDAAMAHINRNKQIIGNYNSQWQGGEHSKHWVEYWGRALPLCSDADTRASVAWGSNV 368
[Close]
BLAST of Rapunzel vs. TrEMBL
Match: V4AXV0 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_238695 PE=4 SV=1)

HSP 1 Score: 159.844 bits (403), Expect = 4.551e-40
Identity = 99/318 (31.13%), Postives = 158/318 (49.69%), Query Frame = 3 
Query:  234 TNLYFGIQAITQMEKFYGEKDF-NKVGKLMADITDETFNVSSSTIFTAFLLGELHATETESFKKASLLLKTRTSRWKEDFNEVRYSIDWAVVQINFEDLENRILTVQRLSTEFMSIDDNEKEFSAKQFLHSYEANYKATGDLLYSVMININQSFSGNLFRASQKFTQFDRKKVQTFMVELAKLLVQAVNIEANFANLKYENDENVKFLNSMWRERFKTLKATMEAVDLDTKEQFRNQYQMDLDEYLMTRES--LTNEQFNQVLYNYLSTKYNWRDWFVVVYAPISGFKDHTVSTCQGTYRFRTNGRNIIVSSVDKEDG 1178
            +N+  G+    ++  F   K F N +GKL   +T     V     F     G+  + E ++ K+    +  R  +    F EV+  IDW+ +Q+ + D+E +I  V +             + +   F+ +YE++Y+ +G  LY  ++N  + F   LF A  K++++DR K QTFM+ L +LL++A  +E  +  LK        + N  WR RF  ++  M + D     Q+  Q   D+D YL+  +   + N  FN  LY +LS KY WRDWF V Y  I+G   H V  C G  +FR +GRN++V+SVD   G
Sbjct:   28 SNIQNGLSLAKELGDFVVAKKFTNVIGKLATAVTPFLGIVGPFVSFIMGFFGQSESAELKAIKRLYTEVDNRFDKVDLQFIEVKRMIDWSKIQVQYSDIEQKINAVNKAFENIYKFSTAAVKTTTSAFVSNYESDYQNSGSKLYDGIMNEGRVFGDGLFTAVVKYSEYDRVKSQTFMLGLLQLLMKAAKLELTYHQLKGHKAALHDYQNQ-WRTRFTHIRIKMMSTDSSIVSQYHTQSGKDIDNYLIKHQKNTMNNNDFNNALYAFLSKKYFWRDWFTVTYNDITGSNVHYVGECGGHIKFRNHGRNVVVASVDSRKG 344
[Close]
BLAST of Rapunzel vs. TrEMBL
Match: V4B6X2 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_237783 PE=4 SV=1)

HSP 1 Score: 159.458 bits (402), Expect = 7.316e-40
Identity = 97/318 (30.50%), Postives = 162/318 (50.94%), Query Frame = 3 
Query:  234 TNLYFGIQAITQMEKFYGEKDF-NKVGKLMADITDETFNVSSSTIFTAFLLGELHATETESFKKASLLLKTRTSRWKEDFNEVRYSIDWAVVQINFEDLENRILTVQRLSTEFMSIDDNEKEFSAKQFLHSYEANYKATGDLLYSVMININQSFSGNLFRASQKFTQFDRKKVQTFMVELAKLLVQAVNIEANFANLKYENDENVKFLNSMWRERFKTLKATMEAVDLDTKEQFRNQYQMDLDEYLM--TRESLTNEQFNQVLYNYLSTKYNWRDWFVVVYAPISGFKDHTVSTCQGTYRFRTNGRNIIVSSVDKEDG 1178
            +N+  G+    ++  F   K+F N +GKL   +T     V     F     G   + E ++ K+    +  R  +    F EV+  IDW+ +QI + D+E +I  V +             + +A  F+ ++E++Y+ +G  LY  ++N  + F   LF A  K++++DR K QTFM+ L +LL++A  +E  +  LK        + N  WR RF  ++  M + D     Q+  Q   D+D YL+   + ++ N  FN +LY +L+ KY+WRDW  + Y  I+G   H V  C G  +FR +GRN++V+SVD+  G
Sbjct:   28 SNIQNGLSVAKELGDFVVAKNFTNVIGKLATAVTPFLGIVGPFVSFIMGFFGHSESAELKAIKRLYTEVDNRFDKVDLQFIEVKRLIDWSKIQIQYSDIEQKINAVNQEFENIYKFSIGAVKTTASAFVKNFESDYQNSGSKLYDGIMNEGRVFGDGLFTAIIKYSEYDRGKSQTFMLGLLQLLMKAAKLELTYHQLKGHTAALHDYQNQ-WRTRFTHIRIKMMSTDNSIVSQYHTQSGKDIDNYLIKHPKNTMNNNDFNNMLYGFLTKKYSWRDWLTITYNDITGSNVHYVGECGGYLKFRNHGRNVVVASVDRRKG 344
[Close]
BLAST of Rapunzel vs. TrEMBL
Match: V3ZRU7 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_235791 PE=4 SV=1)

HSP 1 Score: 146.747 bits (369), Expect = 2.732e-35
Identity = 96/317 (30.28%), Postives = 159/317 (50.16%), Query Frame = 3 
Query:  234 TNLYFGIQAITQMEKFYGEKDF-NKVGKLMADITDETFNVSSSTIFTAFLLGELHATETESFKKASLLLKTRTSRWKEDFNEVRYSIDWAVVQINFEDLENRI--LTVQRLSTEFMSIDDNEKEFSAKQFLHSYEANYKATGDLLYSVMININQSFSGNLFRASQKFTQFDRKKVQTFMVELAKLLVQAVNIEANFANLKYENDENVKFLNSMWRERFKTLKATMEAVDLDTKEQFRNQYQMDLDEYLMT--RESLTNEQFNQVLYNYLSTKYNWRDWFVVVYAPISGFKDHTVSTCQGTYRFRTNGRNIIVSSVDK 1169
            +N+  G+    ++  F   K+F N +GKL   +T     +     F    + +    E ++ K  S  +  R  R    F+EV+  I+W  VQ+ +   E RI  L  +  +    S  D  K  SA  F++SY++ Y+ +G +LY  ++     F G LF +  K T + R K Q FM+ L KLL++A  +E  +  L +   ++V+   + W  RF  ++  M A D    ++F+ Q   D+D+ L    + ++ N  FN ++Y YL+ KY+W+DW  V Y  I+G   H V  C G  +FR +GRN + +SVD+
Sbjct:   28 SNIQHGLSLAKEIGDFVVAKNFTNVIGKLATAVTPYLGIIGPCVSFIMGFVAKTETAELKAIKDLSTKVDNRFDRVDSQFDEVKNLIEWVKVQVQYSSAEQRINALNARFENIYTFSTKDVRKTTSA--FVNSYDSTYQESGTILYQGIMQGGHVFGGGLFTSVVKHTNYHRGKSQAFMLGLLKLLMKAAKLELTYYQLTH---KDVRGNQNQWSTRFSEVRKKMMATDAAIVKEFKTQSGKDIDDLLKNNLKTAMNNNDFNNMIYGYLTEKYSWKDWLTVTYNDITGGNVHYVGECGGYIKFRNHGRNFVAASVDR 339
[Close]
BLAST of Rapunzel vs. TrEMBL
Match: V4ASI2 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_174733 PE=4 SV=1)

HSP 1 Score: 145.206 bits (365), Expect = 1.891e-34
Identity = 103/333 (30.93%), Postives = 166/333 (49.85%), Query Frame = 3 
Query:  234 TNLYFGIQAITQMEKFYGEKDFNKV-GKLMADITDETFNVSSSTIFTAFLLGELHATETESFKKASLLLKTRTSRWKEDFNEVRYSIDWAVVQINFEDLENRILTVQRLSTEFMSIDDNEKEFSAKQFLHSYEANYKATGDLLYSVMININQSFSGNLFRASQKFTQFDRKKVQTFMVELAKLLVQAVNIEANFANLKYENDENVKFLNSMWRERFKTLKATMEAVDLDTKEQFRNQYQMDLDEYLM--TRESLTNEQFNQVLYNYLSTKYNWRDWFVVVYAPISGFKDHTVSTCQGTYRFRTN--GRNIIVSSVDKEDGISAGSHIERNKQI 1217
            +N+  G+ A  ++  F   K+FN V GKL + +T     V     F     G   + E ++ K+ +  +  R  +   +F+EV+  I+W+ +Q+   + E  I  V        +      + +   F+ +++  Y+ +G  LY  ++N  Q F   LF A  K T++DR K QTFM+ L KLL++A  +E  +  LK        + N +W  RF  +++ M + D     Q+  Q   D+D YL+   +  + N  FN +LY++L+ KY WRDWF V Y  I+G K H V  C G  +F +N  GRN++V SVDK  G     HI R + +
Sbjct:   71 SNIQNGLSAAKELGDFIVAKNFNNVIGKLASAVTPYLGIVGPFVSFIMGFFGLSESAELKAIKRLNQDVDNRFDKVDIEFDEVKNLIEWSKIQVQNSNAEQNINAVNTDFEHIYTFSSGAVKTAVSTFVDNFKTVYQDSGTKLYDGIMNEGQMFGDGLFSAVVKHTEYDRGKSQTFMLGLLKLLMKAAKLELAYHQLKGHTAALHDYQN-VWNTRFTHIRSKMMSTDNYIVSQYDTQSGKDIDNYLIKNPKTKMNNNDFNNMLYDFLAKKYFWRDWFTVTYKHINGGKVHKVGQCGGYTKFNSNNSGRNVVVDSVDKLKG-----HINRYRAL 397
[Close]
BLAST of Rapunzel vs. Ensembl Cavefish
Match: rpz4 (uncharacterized LOC103039774 [Source:NCBI gene;Acc:103039774])

HSP 1 Score: 52.373 bits (124), Expect = 6.889e-7
Identity = 57/269 (21.19%), Postives = 108/269 (40.15%), Query Frame = 3 
Query:  297 FNKVGKLMADITDETFNV-SSSTIFTAFLLGELHATETESFKKASLLLKTRTSRWKEDFNEVRYSIDWAVVQINFEDLENRILTVQRLSTEFMSIDDNEKEFSAKQFLHSYEANYKATGD----LLYSVMININQSFSG-NLFRASQKFTQFDRKKVQTFMVELAKLLVQAVNIEANFANLKYENDENVKFLNSMWRERFKTLKATMEAVDLDTKEQFRNQYQMDLDEYLMTRESLTNEQFNQVLYNYLSTKYNWRDWFVVVYAPISGF 1085
            F +  +++A I  + F V + +       L  + + E E  K+    ++ R     E+   +   +  +     +  +E  +    R   + ++     +E   K FL  +    K  GD    +LY+ +   N  FSG ++   S  + Q  R+ V+ F   L +L    +      A LK   DE  + L   W ++ K ++  M AV  D    F +Q ++D+   +       N+Q   ++   L  KY+W  W + V+   +G 
Sbjct:   23 FEQGAEVVASIAGDLFPVFAIAAPIVKLALDNVESKEAEYMKEQFQRVRERLEVVSEEIQRINEEVKKSGADAAYFSVEENLTNQFRKYMDILNAKPKFREVKKKLFLEHFS---KTGGDKNLNILYNAVTGDN--FSGESVLEISLNYEQKSRRAVEDFCARLKQLFCVGLIALMGHAALKGGEDE--EGLLKDWADKMKEVQLKMNAVIEDCINSFPSQAEIDIKRLVRDNRDKPNQQLANMILEALKKKYDWIGWSIRVFNSPTGL 284
[Close]
BLAST of Rapunzel vs. Ensembl Medaka
Match: ENSORLT00000027173.1 (uncharacterized LOC101165669 [Source:NCBI gene;Acc:101165669])

HSP 1 Score: 56.9954 bits (136), Expect = 1.998e-8
Identity = 54/229 (23.58%), Postives = 95/229 (41.48%), Query Frame = 3 
Query:  462 EDFNEVRYSIDWAVVQINFEDLENRILTVQRLSTEFMSIDDNEKEFSAKQFLHSYEANYKATG-DLLYSVMININQSFSGN-LFRASQKFTQFDRKKVQTFMVELAKLLVQAVNIEANFANLKYENDENVKFLNSMWRERFKTLKATMEAVDLDTKEQFRNQYQMDLDEYLMTRESLTNEQFNQVLYNYLSTKYNWRDWFVVVYAPISGFKDHTVS-TCQGTYRFRTNG 1139
            +D   +   I  +++ + F   E  I    R+  EF+      +E   +QFL  + ++      +LLYS+++     ++G  L      + +  R+ V+ +   L  LL   +      A +   N E  K L   W E+  T++  M A   +    F +Q + D +  +M     + +Q    L   L  KY+W  W V VY    GF + T S   +G + F+  G
Sbjct:   78 DDVKRINEEIKKSMLDLGFTSKEENIRHQFRMYMEFVIAKPEFREVKKEQFLIQFSSSEGEKNLNLLYSLLVG---DYNGKPLLETILTYEENSRRSVEEYCARLINLLCIGIIALVGHAAVSG-NGEEEKLLKE-WGEKTTTVQKRMSAAVEECIASFPSQAKSDCERLVMQNGDSSPQQLADTLIEMLKKKYDWVSWSVRVYILNEGFFEKTTSEEIKGQHSFQVTG 301
[Close]
BLAST of Rapunzel vs. Ensembl Medaka
Match: rpz4 (uncharacterized LOC101175680 [Source:NCBI gene;Acc:101175680])

HSP 1 Score: 50.8322 bits (120), Expect = 2.284e-6
Identity = 66/275 (24.00%), Postives = 114/275 (41.45%), Query Frame = 3 
Query:  297 FNKVGKLMADITDETFNVSSSTIFTAFLLGELHATETESFKKASLLLKTRTSRWKEDFNEVRYSIDWAVVQINFEDLENRILTVQRLSTEFMSIDDNEKE--------FSAK-QFLHSYEANY-----KATGDL----LYSVMININQSFSGNLFRASQKFTQFDRKKVQTFMVELAKLLVQAVNIEANFANLKYENDENVKFLNSMWRERFKTLKATMEAVDLDTKEQFRNQYQMDLDEYLMTRESLTNEQFNQVLYNYLSTKYNWRDWFVVVY 1067
            F K  +++A I  + F + S     A  L +L     ES + A +         K+ F +VR  +D  VV    + + N  +   R+ TEF S+++N +          +AK QF    + N+     K+ G+     LYSV++  +  F   L      + +  R+ ++ +   L  L    + I A   +   +     + L   W E+   ++  + AV  +    F NQ + D +  +  +E L N+Q    L   L  KY+W  W V VY
Sbjct:   90 FEKGAEMVASIAGDLFPIFS----VAAPLVQLALDNVESSEAAFM---------KDQFQKVRDRLD--VVSEEIQRI-NEEIKKSRIDTEFFSVEENIRNQFRKYMEILNAKPQFTEVKKQNFLKHFSKSGGNKNLHSLYSVVVG-DGFFGEPLLVTILNYEEKSRRPIEEYCARLKNLF--CIGIIALLGHAAVDGFNEGEELLKEWAEKMTNVQEKINAVVEECIVSFPNQAKTDCERLVRDQEGLDNKQLADALIGILKKKYDWVGWSVRVY 345
[Close]
BLAST of Rapunzel vs. Planmine SMEST
Match: SMESG000052591.1 (SMESG000052591.1)

HSP 1 Score: 872.848 bits (2254), Expect = 0.000e+0
Identity = 421/422 (99.76%), Postives = 421/422 (99.76%), Query Frame = 3 
Query:  165 MQNEFLNHRPFPTNKTYLTGSEMTNLYFGIQAITQMEKFYGEKDFNKVGKLMADITDETFNVSSSTIFTAFLLGELHATETESFKKASLLLKTRTSRWKEDFNEVRYSIDWAVVQINFEDLENRILTVQRLSTEFMSIDDNEKEFSAKQFLHSYEANYKATGDLLYSVMININQSFSGNLFRASQKFTQFDRKKVQTFMVELAKLLVQAVNIEANFANLKYENDENVKFLNSMWRERFKTLKATMEAVDLDTKEQFRNQYQMDLDEYLMTRESLTNEQFNQVLYNYLSTKYNWRDWFVVVYAPISGFKDHTVSTCQGTYRFRTNGRNIIVSSVDKEDGISAGSHIERNKQIIGSYSDWKSDKEYSRGWVSFMDKLLPNCNDADTRLSVAWGSNVWFTNTNLVVYKKNCVNDKSPCFNTFLFA 1430
            MQNEFLNHRPFPTNKTYLTGSEMTNLYFGIQAITQMEKFYGEKDFNKVGKLMADITDETFNVSSSTIFTAFL GELHATETESFKKASLLLKTRTSRWKEDFNEVRYSIDWAVVQINFEDLENRILTVQRLSTEFMSIDDNEKEFSAKQFLHSYEANYKATGDLLYSVMININQSFSGNLFRASQKFTQFDRKKVQTFMVELAKLLVQAVNIEANFANLKYENDENVKFLNSMWRERFKTLKATMEAVDLDTKEQFRNQYQMDLDEYLMTRESLTNEQFNQVLYNYLSTKYNWRDWFVVVYAPISGFKDHTVSTCQGTYRFRTNGRNIIVSSVDKEDGISAGSHIERNKQIIGSYSDWKSDKEYSRGWVSFMDKLLPNCNDADTRLSVAWGSNVWFTNTNLVVYKKNCVNDKSPCFNTFLFA
Sbjct:    1 MQNEFLNHRPFPTNKTYLTGSEMTNLYFGIQAITQMEKFYGEKDFNKVGKLMADITDETFNVSSSTIFTAFLSGELHATETESFKKASLLLKTRTSRWKEDFNEVRYSIDWAVVQINFEDLENRILTVQRLSTEFMSIDDNEKEFSAKQFLHSYEANYKATGDLLYSVMININQSFSGNLFRASQKFTQFDRKKVQTFMVELAKLLVQAVNIEANFANLKYENDENVKFLNSMWRERFKTLKATMEAVDLDTKEQFRNQYQMDLDEYLMTRESLTNEQFNQVLYNYLSTKYNWRDWFVVVYAPISGFKDHTVSTCQGTYRFRTNGRNIIVSSVDKEDGISAGSHIERNKQIIGSYSDWKSDKEYSRGWVSFMDKLLPNCNDADTRLSVAWGSNVWFTNTNLVVYKKNCVNDKSPCFNTFLFA 422
[Close]
BLAST of Rapunzel vs. Planmine SMEST
Match: SMESG000052592.1 (SMESG000052592.1)

HSP 1 Score: 411.379 bits (1056), Expect = 2.455e-140
Identity = 196/422 (46.45%), Postives = 275/422 (65.17%), Query Frame = 3 
Query:  165 MQNEFLNHRPFPTNKTYLTGSEMTNLYFGIQAITQMEKFYGEKDFNKVGKLMADITDETFNVSSSTIFTAFLLGELHATETESFKKASLLLKTRTSRWKEDFNEVRYSIDWAVVQINFEDLENRILTVQRLSTEFMSIDDNEKEFSAKQFLHSYEANYKATGDLLYSVMININQSFSGNLFRASQKFTQFDRKKVQTFMVELAKLLVQAVNIEANFANLKYENDENVKFLNSMWRERFKTLKATMEAVDLDTKEQFRNQYQMDLDEYLMTRESLTNEQFNQVLYNYLSTKYNWRDWFVVVYAPISGFKDHTVSTCQGTYRFRTNGRNIIVSSVDKEDGISAGSHIERNKQIIGSYSDWKSDKEYSRGWVSFMDKLLPNCNDADTRLSVAWGSNVWFTNTNLVVYKKNCVNDKSPCFNTFLFA 1430
            + NE + +R  P  KT LT  E + +  G+Q + Q+ KF  EK+F K    ++ +    F+  S+ +   F+  E+ + E    KK    L  R  +W  +F  ++  IDW+ VQINF D+EN I+T+ ++ +    +    +E  A  F+++YE NYKA GD+L+  M+NI Q +S NL RA+Q + + DR +VQ FMV + KL+ QAV IEA +  LKY N+E  KF  S+W ++   LK  +E +D DTK ++  QYQ DLD  L T  S +NE+F  + Y++ +TKYNWR+WFV+VY  +SGFKDH V TCQGT+RFRT+GRNIIVSSVD+ +G +A +HI RNKQIIG+Y+      E+S+ WV +  ++LP C+DADTR SVAWGSNVWF NT LVVY+KNCV   S C+NT+LFA
Sbjct:   23 IDNELILYRGLPQIKTSLTEKEKSQIMNGVQGVVQLFKFLEEKNFFKTLTQISSVAGPIFSAISAILTVVFMFIEIESPEMALMKKEFANLNNRIDKWGVEFQIIKRKIDWSAVQINFADIENNIITLHKMLSTLPEVPIANRETQAALFINTYEINYKAAGDILHRAMLNIEQIYSVNLLRAAQLYMENDRNQVQEFMVGMMKLITQAVQIEAAYVGLKYNNEEASKFTQSLWEKKLAVLKDKLEQIDTDTKNKYNEQYQKDLDNLLATHYSQSNERFVTIAYDFFATKYNWREWFVLVYKELSGFKDHAVVTCQGTHRFRTHGRNIIVSSVDQSEGDAAMAHINRNKQIIGNYNSQWQGGEHSKHWVEYWGRVLPLCSDADTRASVAWGSNVWFINTRLVVYQKNCVRGSSSCYNTYLFA 444
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BLAST of Rapunzel vs. Planmine SMEST
Match: SMESG000062792.1 (SMESG000062792.1)

HSP 1 Score: 130.183 bits (326), Expect = 5.811e-33
Identity = 88/313 (28.12%), Postives = 152/313 (48.56%), Query Frame = 3 
Query:  462 EDFNEVRYSIDWAVVQINFEDLENRILTVQRLSTEFMSIDDNEKEFSAKQFLHSYEANYKATGDLLYSVMININQSFSGNLFRASQKFTQFDRKKVQTFMVELAKLLVQAVNIEANFANLKYENDENVKFLNSMWRERFKTLKATMEAVDLDTKEQFRNQYQMDLDEYLMTRESLTNEQFNQVLYNYLSTKYNWRDWFVVVYAPISGFKDHTVSTCQGTYRFRTNGRNIIVSSVDKEDGISAGSHIERNKQIIGSYSDWKSDKEYSRGWVS--FMDKLLP---NCNDADTRLSVAWGSNVWF-TNTNLVVYKK 1382
            + F+EV+ +I+W VV++ + + E  I  +Q +         N ++    +F  +Y+  Y    D LY    N  + FS NL  AS +   F R+KV+ F +   +L+++ + +E+ +   K     N  +    W ++   + A ME      K  + +Q Q D +E+ +      N+++ +  YN+L+ K++WR+WF+VVY PI+G  +H VS   G    R+ G NI V+S DKED     + I    Q++ +    +S     +G      MD+L+P     +       + WG+ VWF +N     YKK
Sbjct:  105 QQFDEVKKAINWNVVKVQYFNHETAIGLLQDMILSISDAPSNVRQMMMDRFRITYDQTYYNAADKLYESFTNPQRIFSENLVTASCQTFNFHRRKVEKFFLGNIQLILKGLEVESFYLTTK-NLTSNQDYWAEQWNKKINQISAIMEETINSIKGNWHSQLQKDAEEFNVKNYGKGNKEYLEEFYNFLTDKFSWREWFLVVYNPITGGDNHYVSVYGGIINLRSRGHNIAVASRDKEDNNFNANLIS---QVLDNLP--RSFNTRGKGLAESLLMDQLIPAIQRVHKYSLAAVIEWGAGVWFISNNQFRAYKK 411
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BLAST of Rapunzel vs. Planmine SMEST
Match: SMESG000004912.1 (SMESG000004912.1)

HSP 1 Score: 119.783 bits (299), Expect = 2.774e-29
Identity = 81/293 (27.65%), Postives = 138/293 (47.10%), Query Frame = 3 
Query:  288 EKDFNKVGKLMADITDETFNVSSSTIFTAFLLGELHATETESFKKASLLLKTRTSRWKEDFNEVRYSIDWAVVQINFEDLENRILTVQRLSTEFMSIDDNEKEFSAKQFLHSYEANYKATGDLLYSVMININQSFSGNLFRASQKFTQFDRKKVQTFMVELAKLLVQAVNIEANFANLK-YENDENVKFLNSMWRERFKTLKATMEAVDLDTKEQFRNQYQMDLDEYLMTRESLTNEQFNQVLYNYLSTKYNWRDWFVVVYAPISGFKDHTVSTCQGTYRFRTNGRNIIVSSV 1163
            E D  K GK +A           S      +  E  + E    K+   +L  +     + F EV+  +DW VV+I +   E  +  +Q L         + +  +  +FL SY+  Y    + LY   IN N+ FS NL  A +K+T+  R +++ F +   +L+++ + +E  + NLK + + E  KF    W +R  +L   M+ V     E +  Q  MD++E+       ++E + + LYN+L+ K+  R W V+VY P+    DH +  C G  + + NG N+ VSS+
Sbjct:   51 ESDMYKTGKAIAKWGKPLLEAIGSFAKIIMVQFESDSKELTFMKEQFKILNEKMDYVDQQFEEVKRLMDWNVVKIQYGKYEMHVYMLQHLIQIIADSPASLRHITIDKFLLSYDQVYDNAAENLYQGFINQNKVFSENLVLAIRKYTKDHRYQIEKFFMGNIQLIIKCLEVETFYINLKNFTSIE--KFWQVKWSDRMNSLMDIMKTVSEKISENWEAQSNMDIEEFNAENGKKSHENYAKDLYNFLNDKFRNRIWLVLVYDPLDSGTDHALKACNGISQMQRNGHNVAVSSL 341
[Close]
BLAST of Rapunzel vs. Planmine SMEST
Match: SMESG000046431.1 (SMESG000046431.1)

HSP 1 Score: 49.6766 bits (117), Expect = 4.100e-6
Identity = 53/214 (24.77%), Postives = 90/214 (42.06%), Query Frame = 3 
Query:  582 DNEKE---FSAKQFLHSYEANYKATGDLLYSVMININQSFSGNLFRASQKFTQFDRKKVQTFMVELAKLLVQAVNIEANFANLKYENDENVKFLNSMWRERFKTLKATMEAVDLDTKEQFRNQYQMDLDEYLMTRESLTNEQF---NQVLYNYLSTKYN----WRDWFVVVYAPISGFKDHTVSTCQGTYRF-RTNGRNIIVSSVDKEDGISAG 1190
            DN KE   F AKQ    YE +YK     LY  +   + SF  NL    +   + +   +  F V    L+ + ++IE  +          + F N+    + +T K  ++ +     E   +Q +    EY   + +LT+++    NQV  N L+ + +     R W  V Y   SGF DH  S+     +     G ++ + S  KE+ ++ G
Sbjct:  157 DNTKESYAFFAKQ----YEKSYKFAAANLYDALTGSSGSFGFNLINLYKDHVKCNTVSLMEFCVMGIHLINKGLSIEGAYLGF-------IDF-NAKIEAKMETWKGKLDEISQRC-EGLVSQCEASWKEYAYRQFTLTHDEIGNDNQVFANKLAKELDKVVPHRSWLAVSYNDASGFDDHCYSSYYEAEKIWNKKGHDVTIFSTPKEEALTGG 357
The following BLAST results are available for this feature:
BLAST of Rapunzel vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rapunzel vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rapunzel vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rapunzel vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rpz44.937e-822.74rapunzel 4 [Source:ZFIN;Acc:ZDB-GENE-050417-472][more]
rpz58.306e-628.18rapunzel 5 [Source:ZFIN;Acc:ZDB-GENE-030131-4678][more]
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BLAST of Rapunzel vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rapunzel vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rapunzel vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rapunzel vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0A7NXM51.523e-11445.90Rapunzel (Fragment) OS=Schmidtea mediterranea OX=7... [more]
V4AXV04.551e-4031.13Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
V4B6X27.316e-4030.50Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
V3ZRU72.732e-3530.28Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
V4ASI21.891e-3430.93Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
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BLAST of Rapunzel vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rpz46.889e-721.19uncharacterized LOC103039774 [Source:NCBI gene;Acc... [more]
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BLAST of Rapunzel vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rapunzel vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rapunzel vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rapunzel vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSORLT00000027173.11.998e-823.58uncharacterized LOC101165669 [Source:NCBI gene;Acc... [more]
rpz42.284e-624.00uncharacterized LOC101175680 [Source:NCBI gene;Acc... [more]
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BLAST of Rapunzel vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000052591.10.000e+099.76SMESG000052591.1[more]
SMESG000052592.12.455e-14046.45SMESG000052592.1[more]
SMESG000062792.15.811e-3328.12SMESG000062792.1[more]
SMESG000004912.12.774e-2927.65SMESG000004912.1[more]
SMESG000046431.14.100e-624.77SMESG000046431.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30000350 ID=SMED30000350|Name=Rapunzel|organism=Schmidtea mediterranea sexual|type=transcript|length=1477bp
TTGACTTATAATAAATTAATGACCGTTTAGGATTTAAAATGAGAACCCCA
GTGAATCTTATAAAATCTATTTTTCTTATAATAGTAACCTTTAACATCAT
CGGGCTCGGAAGGATGACATTAATTTCAAGTTCCATCAATGACCCGCTTT
TTCAAATACCTATTATGCAAAACGAATTTTTGAACCATCGTCCATTTCCT
ACCAATAAGACATATTTGACCGGATCTGAGATGACGAATTTATATTTTGG
AATTCAAGCTATAACTCAAATGGAAAAATTCTACGGTGAAAAAGACTTCA
ATAAGGTCGGGAAACTAATGGCTGATATAACTGATGAAACGTTTAATGTT
TCTTCATCAACGATATTTACAGCATTTCTCTTGGGTGAACTACATGCGAC
GGAAACGGAATCCTTTAAAAAGGCCTCTTTACTATTGAAGACTAGAACGA
GTCGATGGAAGGAAGATTTTAATGAAGTGAGATATTCGATTGACTGGGCC
GTTGTCCAGATCAATTTTGAAGACCTCGAAAATAGAATACTTACAGTTCA
AAGGTTATCAACTGAATTCATGAGTATTGACGATAATGAAAAGGAATTTT
CTGCCAAGCAGTTTCTTCATTCATATGAAGCTAATTACAAAGCTACTGGA
GACCTGCTGTACTCGGTGATGATAAATATCAACCAGAGTTTCTCGGGAAA
TCTATTCAGGGCTTCGCAGAAGTTTACCCAGTTTGACCGAAAGAAAGTGC
AAACATTTATGGTAGAACTGGCCAAACTTCTGGTTCAAGCTGTAAATATT
GAAGCTAATTTTGCGAATTTAAAATATGAAAATGATGAAAATGTAAAGTT
TTTGAATTCAATGTGGAGGGAAAGATTCAAAACATTAAAAGCCACAATGG
AAGCCGTTGATCTAGACACAAAAGAACAATTTAGAAACCAATATCAAATG
GATCTAGATGAATATTTAATGACAAGAGAATCTTTGACAAACGAACAATT
TAATCAGGTTTTATATAATTATCTGTCCACAAAATATAACTGGAGGGATT
GGTTTGTCGTTGTCTACGCACCGATTTCCGGTTTTAAAGATCATACAGTT
TCCACGTGTCAAGGGACCTATAGATTCAGAACAAATGGCCGAAACATTAT
TGTTTCAAGCGTAGATAAGGAAGACGGTATCTCAGCAGGCTCTCACATAG
AACGAAATAAGCAAATAATCGGTAGCTATTCCGATTGGAAATCGGACAAA
GAATATTCCAGAGGCTGGGTATCTTTTATGGACAAATTACTTCCCAATTG
TAATGACGCGGATACGAGGCTGTCTGTTGCTTGGGGTAGTAACGTTTGGT
TTACGAATACAAATTTGGTTGTTTACAAGAAGAACTGTGTCAATGATAAA
TCACCTTGTTTTAATACTTTTCTTTTTGCCTGAAAATATTAATAAATATA
AAATTTTCTCTTTTTGTTCAAAAAAAA
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protein sequence of SMED30000350-orf-1

>SMED30000350-orf-1 ID=SMED30000350-orf-1|Name=SMED30000350-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=465bp
MRTPVNLIKSIFLIIVTFNIIGLGRMTLISSSINDPLFQIPIMQNEFLNH
RPFPTNKTYLTGSEMTNLYFGIQAITQMEKFYGEKDFNKVGKLMADITDE
TFNVSSSTIFTAFLLGELHATETESFKKASLLLKTRTSRWKEDFNEVRYS
IDWAVVQINFEDLENRILTVQRLSTEFMSIDDNEKEFSAKQFLHSYEANY
KATGDLLYSVMININQSFSGNLFRASQKFTQFDRKKVQTFMVELAKLLVQ
AVNIEANFANLKYENDENVKFLNSMWRERFKTLKATMEAVDLDTKEQFRN
QYQMDLDEYLMTRESLTNEQFNQVLYNYLSTKYNWRDWFVVVYAPISGFK
DHTVSTCQGTYRFRTNGRNIIVSSVDKEDGISAGSHIERNKQIIGSYSDW
KSDKEYSRGWVSFMDKLLPNCNDADTRLSVAWGSNVWFTNTNLVVYKKNC
VNDKSPCFNTFLFA*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0000419prepharyngeal region
PLANA:0000420parapharyngeal region
PLANA:0002075pigment cell
Vocabulary: INTERPRO
TermDefinition
IPR039051SE-CTX-like
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 299..319
NoneNo IPR availableTMHMMTMhelixcoord: 7..29
IPR039051SE-cephalotoxin-likePANTHERPTHR40472FAMILY NOT NAMEDcoord: 95..377