Tubulin beta chain

Overview
NameTubulin beta chain
Smed IDSMED30000332
Length (bp)1766
Neoblast Clusters

Zeng et. al., 2018




 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020




SMED30000332

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
testis primordiumSMED30000332SMESG000057129.1 PL04005B1A02_SE3GPL10652PMID:20844018
Wang et al., 2010
whole organism juvenile cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Tubulin beta chain vs. Ensembl Human
Match: TUBB4B (tubulin beta 4B class IVb [Source:HGNC Symbol;Acc:HGNC:20771])

HSP 1 Score: 565.459 bits (1456), Expect = 0.000e+0
Identity = 267/429 (62.24%), Postives = 346/429 (80.65%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            MREI+++Q GQCGN IG KFWE+I++EHGI+  TG Y GDSDLQL+RI VY+N    GKYVPR+V+VDL+  T+D + +   G++F+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ +  V  L   MF  +NMMAAC+P +G+YLT+A +FRGRMS KEV+E +L V++KN S F++WIP+N+KTAVCDIPPRG ++SAT I N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y++
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDP-TGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Human
Match: TUBB (tubulin beta class I [Source:HGNC Symbol;Acc:HGNC:20778])

HSP 1 Score: 561.607 bits (1446), Expect = 0.000e+0
Identity = 264/429 (61.54%), Postives = 344/429 (80.19%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            MREI++IQ GQCGN IG KFWE+I++EHGI+  TG Y GDSDLQL RI VY+N    GKYVPR+++VDL+  T+D + +   G++F+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ +  V  L   +F  +NMMAAC+P +G+YLT+A +FRGRMS KEV+E +L V++KN S F++WIP+N+KTAVCDIPPRG +++ T I N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y++
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDP-TGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Human
Match: TUBB (tubulin beta class I [Source:HGNC Symbol;Acc:HGNC:20778])

HSP 1 Score: 561.607 bits (1446), Expect = 0.000e+0
Identity = 264/429 (61.54%), Postives = 344/429 (80.19%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            MREI++IQ GQCGN IG KFWE+I++EHGI+  TG Y GDSDLQL RI VY+N    GKYVPR+++VDL+  T+D + +   G++F+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ +  V  L   +F  +NMMAAC+P +G+YLT+A +FRGRMS KEV+E +L V++KN S F++WIP+N+KTAVCDIPPRG +++ T I N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y++
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDP-TGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Human
Match: TUBB4A (tubulin beta 4A class IVa [Source:HGNC Symbol;Acc:HGNC:20774])

HSP 1 Score: 561.607 bits (1446), Expect = 0.000e+0
Identity = 265/429 (61.77%), Postives = 344/429 (80.19%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            MREI+++Q GQCGN IG KFWE+I++EHGI+  TG Y GDSDLQL+RI VY+N    G YVPR+V+VDL+  T+D + +   G++F+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ +  V  L   MF  +NMMAAC+P +G+YLT+A +FRGRMS KEV+E +L V+ KN S F++WIP+N+KTAVCDIPPRG +++AT I N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y++
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDP-TGTYHGDSDLQLERINVYYNEATGGNYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDAVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEFPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLSVQSKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Human
Match: TUBB (tubulin beta class I [Source:HGNC Symbol;Acc:HGNC:20778])

HSP 1 Score: 561.607 bits (1446), Expect = 0.000e+0
Identity = 264/429 (61.54%), Postives = 344/429 (80.19%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            MREI++IQ GQCGN IG KFWE+I++EHGI+  TG Y GDSDLQL RI VY+N    GKYVPR+++VDL+  T+D + +   G++F+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ +  V  L   +F  +NMMAAC+P +G+YLT+A +FRGRMS KEV+E +L V++KN S F++WIP+N+KTAVCDIPPRG +++ T I N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y++
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDP-TGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Celegans
Match: tbb-4 (Tubulin beta-4 chain [Source:UniProtKB/Swiss-Prot;Acc:P41937])

HSP 1 Score: 571.237 bits (1471), Expect = 0.000e+0
Identity = 274/439 (62.41%), Postives = 348/439 (79.27%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEEKDNGDHGEDD 1379
            MREI++IQ GQCGN IG KFWE+I++EHGI+  TG Y GDSDLQL+RI VY+N  + GKYVPR+ +VDL+  T+D +     G+LF+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQM + LGGGTGSG+G+L+++KIREE+PDRI+ T S+VPSPK+S+TVVEPYN TL  + L   +DETFCIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ S  V  L   MF  +NMMAAC+P +G+YLT+A +FRGRMS KEV+E +L V++KN S F++WIP+N+KTAVCDIPPRG +++AT + N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y+E    D GE D
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDP-TGAYNGDSDLQLERINVYYNEASGGKYVPRACLVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDNVLDVVRKEAESCDCLQGFQMTHSLGGGTGSGMGTLLISKIREEYPDRIMMTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSMTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-SLTVPELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGVKMAATFVGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATADDEGEFD 437          
BLAST of Tubulin beta chain vs. Ensembl Celegans
Match: ben-1 (Tubulin beta chain [Source:UniProtKB/TrEMBL;Acc:Q18817])

HSP 1 Score: 566.229 bits (1458), Expect = 0.000e+0
Identity = 268/440 (60.91%), Postives = 351/440 (79.77%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEEKDNGDHGEDDG 1382
            MREI+++Q GQCGN IG KFWE+I++EHGI    G Y+G+SDLQL+RI VY+N  N GKYVPR+V+VDL+  T+D + +   G+LF+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI+S+ S+VPSPK+S+TVVEPYN TL  + L   +DETFCIDNEALY IC   LKL NPT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPL+++  + Y+ +  V  L   MF  +NMMAAC+P +G+YLT+A +FRGRMS +EV++ ++ V++KN S F++WIP+N+KTAVCDIPPRG ++SAT I N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y+E    + GE DG
Sbjct:    1 MREIVHVQAGQCGNQIGAKFWEVISDEHGIQP-DGTYKGESDLQLERINVYYNEANGGKYVPRAVLVDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDNVLDVVRKEAEGCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMSSFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLSNPTYGDLNHLVSVTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLSAKGAQAYR-ALTVAELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMREVDDQMMNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATAEEDGELDG 438          
BLAST of Tubulin beta chain vs. Ensembl Celegans
Match: tbb-1 (Tubulin beta chain [Source:UniProtKB/TrEMBL;Acc:O17921])

HSP 1 Score: 564.688 bits (1454), Expect = 0.000e+0
Identity = 266/439 (60.59%), Postives = 352/439 (80.18%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEEKDNGDHGEDD 1379
            MREI+++Q GQCGN IG+KFWE+I++EHGI    G ++G+SDLQL+RI+VY+N  NNGKYVPR+V+VDL+  T+D + +   G+LF+PD F+ G S A NN+AKG YT+GAE++D ++++IR + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI+S+ S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL NPT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPL+++  + Y+ +  V  L   MF  +NMMAAC+P +G+YLT+A +FRGRMS +EV+E +L V++KN S F++WIP+N+KTAVCDIPPRG +++AT + N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y+E    D   D+
Sbjct:    1 MREIVHVQAGQCGNQIGSKFWEVISDEHGIQP-DGTFKGESDLQLERIDVYYNEANNGKYVPRAVLVDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDNVLDVIRKEAEGCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEFPDRIMSSFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICYRTLKLTNPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLSAKGAQAYR-ALTVAELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMREVDEQMLSVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFVGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLISEYQQYQEATAEDEPLDE 437          
BLAST of Tubulin beta chain vs. Ensembl Celegans
Match: tbb-1 (Tubulin beta chain [Source:UniProtKB/TrEMBL;Acc:O17921])

HSP 1 Score: 564.688 bits (1454), Expect = 0.000e+0
Identity = 266/439 (60.59%), Postives = 352/439 (80.18%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEEKDNGDHGEDD 1379
            MREI+++Q GQCGN IG+KFWE+I++EHGI    G ++G+SDLQL+RI+VY+N  NNGKYVPR+V+VDL+  T+D + +   G+LF+PD F+ G S A NN+AKG YT+GAE++D ++++IR + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI+S+ S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL NPT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPL+++  + Y+ +  V  L   MF  +NMMAAC+P +G+YLT+A +FRGRMS +EV+E +L V++KN S F++WIP+N+KTAVCDIPPRG +++AT + N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y+E    D   D+
Sbjct:    1 MREIVHVQAGQCGNQIGSKFWEVISDEHGIQP-DGTFKGESDLQLERIDVYYNEANNGKYVPRAVLVDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDNVLDVIRKEAEGCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEFPDRIMSSFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICYRTLKLTNPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLSAKGAQAYR-ALTVAELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMREVDEQMLSVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFVGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLISEYQQYQEATAEDEPLDE 437          
BLAST of Tubulin beta chain vs. Ensembl Celegans
Match: tbb-2 (Tubulin beta-2 chain [Source:UniProtKB/Swiss-Prot;Acc:P52275])

HSP 1 Score: 563.918 bits (1452), Expect = 0.000e+0
Identity = 267/445 (60.00%), Postives = 355/445 (79.78%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEEKDNGDHGEDDGNPEAD 1397
            MREI+++Q GQCGN IG+KFWE+I++EHGI    G ++G++DLQL+RI+VY+N  NNGKYVPR+V+VDL+  T+D + +   G+LF+PD F+ G S A NN+AKG YT+GAE++D ++++IR + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI+S+ S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL NPT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPL+++  + Y+ +  V  L   MF  +NMMAAC+P +G+YLT+A +FRGRMS +EV+E +L V++KN S F++WIP+N+KTAVCDIPPRG +++AT + N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y+E    D  + DG  E +
Sbjct:    1 MREIVHVQAGQCGNQIGSKFWEVISDEHGIQP-DGTFKGETDLQLERIDVYYNEANNGKYVPRAVLVDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDNVLDVIRKEAEGCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMSSFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICYRTLKLTNPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLSAKGTQAYR-ALTVAELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMREVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFVGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLISEYQQYQEATAED--DVDGYAEGE 441          
BLAST of Tubulin beta chain vs. Ensembl Fly
Match: betaTub56D (gene:FBgn0284243 transcript:FBtr0086536)

HSP 1 Score: 567.77 bits (1462), Expect = 0.000e+0
Identity = 269/429 (62.70%), Postives = 347/429 (80.89%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            MREI++IQ GQCGN IG KFWE+I++EHGI+  TG Y GDSDLQL+RI VY+N  + GKYVPR+V+VDL+  T+D + +   G++F+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ +  V  L   MF  +NMMAAC+P +G+YLT+A IFRGRMS KEV+E +L +++KN S F++WIP+N+KTAVCDIPPRG ++SAT I N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y+E
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEIISDEHGID-ATGAYHGDSDLQLERINVYYNEASGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQE 427          
BLAST of Tubulin beta chain vs. Ensembl Fly
Match: betaTub85D (gene:FBgn0003889 transcript:FBtr0082046)

HSP 1 Score: 558.91 bits (1439), Expect = 0.000e+0
Identity = 267/429 (62.24%), Postives = 343/429 (79.95%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            MREI++IQ GQCGN IG KFWE+I++EH I+  TG Y GDSDLQL+RI VY+N     KYVPR+++VDL+  T+D + +   G++F+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ +  V  L   MF  +NMMAAC+P +G+YLT+A IFRGRMS KEV+E +L +++KN S F++WIP+N KTAVCDIPPRG ++SAT I N+TAIQE F+RV EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y+E
Sbjct:    1 MREIVHIQAGQCGNQIGGKFWEVISDEHCID-ATGTYYGDSDLQLERINVYYNEATGAKYVPRAILVDLEPGTMDSVRSGAFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESEGCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNIQNKNSSFFVEWIPNNCKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQE 427          
BLAST of Tubulin beta chain vs. Ensembl Fly
Match: betaTub60D (gene:FBgn0003888 transcript:FBtr0343443)

HSP 1 Score: 554.288 bits (1427), Expect = 0.000e+0
Identity = 265/448 (59.15%), Postives = 353/448 (78.79%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGN-----NGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEEKDNGDHGEDDGNPE 1391
            MREI+N+Q GQCGN IG KFWE+I+ EHGI++  G+Y GDSDLQL+R+ VY+N  +      GKYVPR++++DL+  T++ + +   G+LF+PD F+ G S A NN+AKG YT+GAE++D +++++R + E+CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LK+ NP++ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ +  V  L   MF  +NMMAAC+P +G+YLT+A +FRGRMS KEV+E +L V++KN S F++WIP+N+KTAVCDIPP+G ++S+T I N TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y+E    D  + + N E
Sbjct:    1 MREIVNLQAGQCGNQIGAKFWEIISEEHGIDS-NGIYVGDSDLQLERVSVYYNEASVTRSSGGKYVPRAILLDLEPGTMESVRSGPYGQLFRPDNFVYGQSGAGNNWAKGHYTEGAELVDNVLDVVRKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSIHQLVENTDETYCIDNEALYDICFRTLKVSNPSYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLAVQNKNSSYFVEWIPNNVKTAVCDIPPKGLKMSSTFIGNTTAIQELFKRISEQFSAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATADDEFDPEVNQE 446          
BLAST of Tubulin beta chain vs. Ensembl Fly
Match: betaTub60D (gene:FBgn0003888 transcript:FBtr0072270)

HSP 1 Score: 553.903 bits (1426), Expect = 0.000e+0
Identity = 265/449 (59.02%), Postives = 353/449 (78.62%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGN------NGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEEKDNGDHGEDDGNPE 1391
            MREI+N+Q GQCGN IG KFWE+I+ EHGI++  G+Y GDSDLQL+R+ VY+N  +       GKYVPR++++DL+  T++ + +   G+LF+PD F+ G S A NN+AKG YT+GAE++D +++++R + E+CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LK+ NP++ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ +  V  L   MF  +NMMAAC+P +G+YLT+A +FRGRMS KEV+E +L V++KN S F++WIP+N+KTAVCDIPP+G ++S+T I N TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y+E    D  + + N E
Sbjct:    1 MREIVNLQAGQCGNQIGAKFWEIISEEHGIDS-NGIYVGDSDLQLERVSVYYNEASAVTRSSGGKYVPRAILLDLEPGTMESVRSGPYGQLFRPDNFVYGQSGAGNNWAKGHYTEGAELVDNVLDVVRKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSIHQLVENTDETYCIDNEALYDICFRTLKVSNPSYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLAVQNKNSSYFVEWIPNNVKTAVCDIPPKGLKMSSTFIGNTTAIQELFKRISEQFSAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATADDEFDPEVNQE 447          
BLAST of Tubulin beta chain vs. Ensembl Fly
Match: betaTub97EF (gene:FBgn0003890 transcript:FBtr0300611)

HSP 1 Score: 551.977 bits (1421), Expect = 0.000e+0
Identity = 265/439 (60.36%), Postives = 346/439 (78.82%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEEKDNGDHGEDD 1379
            MREI+++Q GQCGN IG+KFWE+I++EHGI+   G Y G+S LQ +RI+VY+N  ++GKYVPR+V++DL+  T+D +  S +G+LF+PD F+ G S A NN+AKG YT+GAE+ID ++E++R + E CD LQGFQ+ + LGGGTGSGLG+L+++KIREE+PDRI+++ S+VPSPK+S+TVVEPYN TL  + L   +DETFCIDNEALY IC   LKL +PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLT++ ++ Y+ +  V  L   MF  +NMM AC+P +G+YLT+A IFRG MS KEV+  +  V+ KN S F++WIP+N+K AVCDIPPRG ++SAT I N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y+E    D  E D
Sbjct:    1 MREIVHLQAGQCGNQIGSKFWEIISDEHGIDP-NGYYHGESALQHERIDVYYNEASSGKYVPRAVLIDLEPGTMDSVRQSPVGQLFRPDNFVYGQSGAGNNWAKGHYTEGAELIDSVLEVLRKESEGCDCLQGFQLAHSLGGGTGSGLGTLLISKIREEYPDRIMNSFSVVPSPKVSDTVVEPYNATLSIHQLVENTDETFCIDNEALYDICFRTLKLSSPTYGDLNHLVSVTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTAKGSQQYR-ALTVAELTQQMFDAKNMMTACDPRHGRYLTVACIFRGPMSMKEVDTQMYNVQSKNSSYFVEWIPNNVKVAVCDIPPRGLKMSATFIGNSTAIQEIFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLISEYQQYQEATADDEVEFD 437          
BLAST of Tubulin beta chain vs. Ensembl Zebrafish
Match: zgc:153426 (zgc:153426 [Source:ZFIN;Acc:ZDB-GENE-060929-220])

HSP 1 Score: 565.844 bits (1457), Expect = 0.000e+0
Identity = 266/429 (62.00%), Postives = 346/429 (80.65%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            MREI+++Q GQCGN IG KFWE+I++EHGI+  TG Y GDSDLQL RI VY+N    GKYVPR+++VDL+  T+D + +   G++F+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ S +V  L   MF  +NMMAAC+P +G+YLT+A +FRGRMS KEV+E +L V++KN S F++WIP+N+KTAVCDIPPRG +++AT I N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y++
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDP-TGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-SLSVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Zebrafish
Match: zgc:65894 (zgc:65894 [Source:ZFIN;Acc:ZDB-GENE-030131-7741])

HSP 1 Score: 565.459 bits (1456), Expect = 0.000e+0
Identity = 268/429 (62.47%), Postives = 346/429 (80.65%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            MREIL++Q GQCGN IG KFWE+I++EHGI+  TG Y GDSDLQL RI VY+N  + GKYVPR+++VDL+  T+D + +   G++F+PD F+ G S A NN+AKG YT+GAE++D +M+++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+   +V  L   MF  +NMMAAC+P +G+YLT+A +FRGRMS KEV+E +L V++KN S F++WIP+N+KTAVCDIPPRG ++SAT I N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y++
Sbjct:    1 MREILHLQAGQCGNQIGAKFWEVISDEHGIDP-TGSYHGDSDLQLDRISVYYNEASGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVMDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEFPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-VLSVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Zebrafish
Match: zgc:55461 (zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879])

HSP 1 Score: 565.074 bits (1455), Expect = 0.000e+0
Identity = 266/429 (62.00%), Postives = 346/429 (80.65%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            MREI+++Q GQCGN IG KFWE+I++EHGI+  TG Y GDSDLQL+RI VY+N    GKYVPR+V+VDL+  T+D + +   G++F+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ +  V  L   MF  +NMMAAC+P +G+YLT+A +FRGRMS KEV+E +L V++KN S F++WIP+N+KTAVCDIPPRG +++AT I N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y++
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDP-TGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Zebrafish
Match: zgc:55461 (zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879])

HSP 1 Score: 565.074 bits (1455), Expect = 0.000e+0
Identity = 266/429 (62.00%), Postives = 346/429 (80.65%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            MREI+++Q GQCGN IG KFWE+I++EHGI+  TG Y GDSDLQL+RI VY+N    GKYVPR+V+VDL+  T+D + +   G++F+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ +  V  L   MF  +NMMAAC+P +G+YLT+A +FRGRMS KEV+E +L V++KN S F++WIP+N+KTAVCDIPPRG +++AT I N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y++
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDP-TGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Zebrafish
Match: zgc:55461 (zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879])

HSP 1 Score: 565.074 bits (1455), Expect = 0.000e+0
Identity = 266/429 (62.00%), Postives = 346/429 (80.65%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            MREI+++Q GQCGN IG KFWE+I++EHGI+  TG Y GDSDLQL+RI VY+N    GKYVPR+V+VDL+  T+D + +   G++F+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ +  V  L   MF  +NMMAAC+P +G+YLT+A +FRGRMS KEV+E +L V++KN S F++WIP+N+KTAVCDIPPRG +++AT I N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y++
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDP-TGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Xenopus
Match: ubxn11 (UBX domain protein 11 [Source:Xenbase;Acc:XB-GENE-985480])

HSP 1 Score: 566.614 bits (1459), Expect = 0.000e+0
Identity = 268/429 (62.47%), Postives = 347/429 (80.89%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            MREI+++Q GQCGN IG KFWE+I++EHGI+  TG Y GDSDLQL+RI VY+N  + GKYVPR+V+VDL+  T+D + +   G++F+PD F+ G S A NN+AKG YT+GAE++D +M+++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ S  V  L   MF  +NMMAAC+P +G+YLT+A +FRGRMS KEV+E +L V++KN S F++WIP+N+KTAVCDIPPRG +++AT I N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y++
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDP-TGTYHGDSDLQLERINVYYNEASGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVMDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-SLTVPELTQQMFDSKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Xenopus
Match: reps2 (RALBP1 associated Eps domain containing 2 [Source:Xenbase;Acc:XB-GENE-985000])

HSP 1 Score: 566.229 bits (1458), Expect = 0.000e+0
Identity = 267/429 (62.24%), Postives = 346/429 (80.65%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            MREI+++Q GQCGN IG KFWE+I++EHGI+  TG Y GDSDLQL+RI VY+N    GKYVPR+V+VDL+  T+D + +   G++F+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ +  V  L   MF  +NMMAAC+P +G+YLT+A +FRGRMS KEV+E +L V++KN S F++WIP+N+KTAVCDIPPRG ++SAT I N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y++
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDP-TGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Xenopus
Match: tubb (tubulin beta class I [Source:NCBI gene;Acc:448742])

HSP 1 Score: 561.607 bits (1446), Expect = 0.000e+0
Identity = 264/429 (61.54%), Postives = 344/429 (80.19%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            MREI++IQ GQCGN IG KFWE+I++EHGI+  TG Y GDSDLQL RI VY+N    GKYVPR+++VDL+  T+D + +   G++F+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ +  V  L   +F  +NMMAAC+P +G+YLT+A +FRGRMS KEV+E +L V++KN S F++WIP+N+KTAVCDIPPRG +++ T I N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y++
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDP-TGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Xenopus
Match: znf330 (zinc finger protein 330 [Source:Xenbase;Acc:XB-GENE-1010548])

HSP 1 Score: 560.066 bits (1442), Expect = 0.000e+0
Identity = 265/429 (61.77%), Postives = 345/429 (80.42%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            MREI++IQ GQCGN IG KFWE+I++EHGI+  TG Y GDSDLQL+RI VY+N     K+VPR+++VDL+  T+D + +   G++F+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S++PSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ +  V  L   MF  +NMMAAC+P +G+YLT+A IFRGRMS KEV+E +L V++KN S F++WIP+N+KTAVCDIPPRG ++SAT I N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y++
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDP-TGSYHGDSDLQLERINVYYNEATGNKFVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Xenopus
Match: tubb3 (tubulin beta 3 class III [Source:NCBI gene;Acc:395045])

HSP 1 Score: 556.214 bits (1432), Expect = 0.000e+0
Identity = 266/442 (60.18%), Postives = 351/442 (79.41%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE---KDNGDHGEDD 1379
            MREI++IQ GQCGN IG KFWE+I++EHGI+   G Y GDSDLQL+RI VY+N  ++ KYVPR+++VDL+  T+D + +   G LF+PD FI G S A NN+AKG YT+GAE++D +++++R + E+CD LQGFQ+ + LGGGTGSG+G+L+++K+REE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TT LRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLT+R ++ Y+ +  V  L   MF  +NMMAAC+P +G+YLT+AT+FRGRMS KEV+E +L ++ KN S F++WIP+N+K AVCDIPPRG ++S+T I N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y++   ++ G+  EDD
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPC-GNYIGDSDLQLERISVYYNEASSHKYVPRAILVDLEPGTMDSVRSGAFGHLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKVREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSIHQLVENTDETYCIDNEALYDICFRTLKLATPTYGDLNHLVSATMSGVTTSLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTARGSQQYR-ALTVPELTQQMFDAKNMMAACDPRHGRYLTVATVFRGRMSMKEVDEQMLAIQSKNSSYFVEWIPNNVKVAVCDIPPRGLKMSSTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEEGEMYEDD 440          
BLAST of Tubulin beta chain vs. Ensembl Mouse
Match: Tubb4b (tubulin, beta 4B class IVB [Source:MGI Symbol;Acc:MGI:1915472])

HSP 1 Score: 565.459 bits (1456), Expect = 0.000e+0
Identity = 267/429 (62.24%), Postives = 346/429 (80.65%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            MREI+++Q GQCGN IG KFWE+I++EHGI+  TG Y GDSDLQL+RI VY+N    GKYVPR+V+VDL+  T+D + +   G++F+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ +  V  L   MF  +NMMAAC+P +G+YLT+A +FRGRMS KEV+E +L V++KN S F++WIP+N+KTAVCDIPPRG ++SAT I N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y++
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDP-TGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Mouse
Match: Tubb2b (tubulin, beta 2B class IIB [Source:MGI Symbol;Acc:MGI:1920960])

HSP 1 Score: 561.607 bits (1446), Expect = 0.000e+0
Identity = 266/429 (62.00%), Postives = 345/429 (80.42%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            MREI++IQ GQCGN IG KFWE+I++EHGI+  TG Y GDSDLQL+RI VY+N     KYVPR+++VDL+  T+D + +   G++F+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S++PSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ +  V  L   MF  +NMMAAC+P +G+YLT+A IFRGRMS KEV+E +L V++KN S F++WIP+N+KTAVCDIPPRG ++SAT I N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y++
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDP-TGSYHGDSDLQLERINVYYNEATGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Mouse
Match: Tubb5 (tubulin, beta 5 class I [Source:MGI Symbol;Acc:MGI:107812])

HSP 1 Score: 561.607 bits (1446), Expect = 0.000e+0
Identity = 264/429 (61.54%), Postives = 344/429 (80.19%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            MREI++IQ GQCGN IG KFWE+I++EHGI+  TG Y GDSDLQL RI VY+N    GKYVPR+++VDL+  T+D + +   G++F+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ +  V  L   +F  +NMMAAC+P +G+YLT+A +FRGRMS KEV+E +L V++KN S F++WIP+N+KTAVCDIPPRG +++ T I N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y++
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDP-TGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Mouse
Match: Tubb4a (tubulin, beta 4A class IVA [Source:MGI Symbol;Acc:MGI:107848])

HSP 1 Score: 561.607 bits (1446), Expect = 0.000e+0
Identity = 265/429 (61.77%), Postives = 344/429 (80.19%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            MREI+++Q GQCGN IG KFWE+I++EHGI+  TG Y GDSDLQL+RI VY+N    G YVPR+V+VDL+  T+D + +   G++F+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ +  V  L   MF  +NMMAAC+P +G+YLT+A +FRGRMS KEV+E +L V+ KN S F++WIP+N+KTAVCDIPPRG +++AT I N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y++
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDP-TGTYHGDSDLQLERINVYYNEATGGNYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDAVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEFPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLSVQSKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Mouse
Match: Tubb3 (tubulin, beta 3 class III [Source:MGI Symbol;Acc:MGI:107813])

HSP 1 Score: 558.14 bits (1437), Expect = 0.000e+0
Identity = 268/448 (59.82%), Postives = 352/448 (78.57%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEEKDNGDHGE----DDGNPEA 1394
            MREI++IQ GQCGN IG KFWE+I++EHGI+  +G Y GDSDLQL+RI VY+N  ++ KYVPR+++VDL+  T+D + +   G LF+PD FI G S A NN+AKG YT+GAE++D +++++R + E+CD LQGFQ+ + LGGGTGSG+G+L+++K+REE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TT LRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLT+R ++ Y+ +  V  L   MF  +NMMAAC+P +G+YLT+AT+FRGRMS KEV+E +L ++ KN S F++WIP+N+K AVCDIPPRG ++S+T I N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y++    + GE    DD   EA
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDP-SGNYVGDSDLQLERISVYYNEASSHKYVPRAILVDLEPGTMDSVRSGAFGHLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKVREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSIHQLVENTDETYCIDNEALYDICFRTLKLATPTYGDLNHLVSATMSGVTTSLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTARGSQQYR-ALTVPELTQQMFDAKNMMAACDPRHGRYLTVATVFRGRMSMKEVDEQMLAIQSKNSSYFVEWIPNNVKVAVCDIPPRGLKMSSTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEEGEMYEDDDEESEA 446          
BLAST of Tubulin beta chain vs. UniProt/SwissProt
Match: sp|P41937|TBB4_CAEEL (Tubulin beta-4 chain OS=Caenorhabditis elegans OX=6239 GN=tbb-4 PE=3 SV=1)

HSP 1 Score: 571.237 bits (1471), Expect = 0.000e+0
Identity = 274/439 (62.41%), Postives = 348/439 (79.27%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEEKDNGDHGEDD 1379
            MREI++IQ GQCGN IG KFWE+I++EHGI+  TG Y GDSDLQL+RI VY+N  + GKYVPR+ +VDL+  T+D +     G+LF+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQM + LGGGTGSG+G+L+++KIREE+PDRI+ T S+VPSPK+S+TVVEPYN TL  + L   +DETFCIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ S  V  L   MF  +NMMAAC+P +G+YLT+A +FRGRMS KEV+E +L V++KN S F++WIP+N+KTAVCDIPPRG +++AT + N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y+E    D GE D
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDP-TGAYNGDSDLQLERINVYYNEASGGKYVPRACLVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDNVLDVVRKEAESCDCLQGFQMTHSLGGGTGSGMGTLLISKIREEYPDRIMMTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSMTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-SLTVPELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGVKMAATFVGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATADDEGEFD 437          
BLAST of Tubulin beta chain vs. UniProt/SwissProt
Match: sp|Q9NFZ6|TBB2_ECHMU (Tubulin beta-2 chain OS=Echinococcus multilocularis OX=6211 GN=TUB-2 PE=3 SV=1)

HSP 1 Score: 569.696 bits (1467), Expect = 0.000e+0
Identity = 271/429 (63.17%), Postives = 347/429 (80.89%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            MREI++IQ GQCGN IG KFWE+I++EHGI+  TG Y GDSDLQL+RI VY+N  + GKYVPR+++VDL+  T+D +     G++F+PD F+ G S A NN+AKG YT+GAE++D ++++IR + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI+ T S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL NPT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ + +V  L   MF  +NMMAAC+P +G+YLT+A IFRGRMS KEV+E +L V++KN S F++WIP+N+KTAVCDIPPRG ++SAT + N+TAIQE FRRV EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y++
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDP-TGTYHGDSDLQLERINVYYNEASGGKYVPRAILVDLEPGTMDSVRAGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVIRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMVTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLSNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALSVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFVGNSTAIQELFRRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. UniProt/SwissProt
Match: sp|Q27U48|TBB1_GLOMM (Tubulin beta-1 chain OS=Glossina morsitans morsitans OX=37546 PE=2 SV=1)

HSP 1 Score: 567.77 bits (1462), Expect = 0.000e+0
Identity = 269/429 (62.70%), Postives = 347/429 (80.89%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            MREI++IQ GQCGN IG KFWE+I++EHGI+  TG Y GDSDLQL+RI VY+N  + GKYVPR+V+VDL+  T+D + +   G++F+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ +  V  L   MF  +NMMAAC+P +G+YLT+A IFRGRMS KEV+E +L +++KN S F++WIP+N+KTAVCDIPPRG ++SAT I N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y+E
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEIISDEHGID-ATGAYHGDSDLQLERINVYYNEASGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQE 427          
BLAST of Tubulin beta chain vs. UniProt/SwissProt
Match: sp|Q24560|TBB1_DROME (Tubulin beta-1 chain OS=Drosophila melanogaster OX=7227 GN=betaTub56D PE=1 SV=2)

HSP 1 Score: 567.77 bits (1462), Expect = 0.000e+0
Identity = 269/429 (62.70%), Postives = 347/429 (80.89%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            MREI++IQ GQCGN IG KFWE+I++EHGI+  TG Y GDSDLQL+RI VY+N  + GKYVPR+V+VDL+  T+D + +   G++F+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ +  V  L   MF  +NMMAAC+P +G+YLT+A IFRGRMS KEV+E +L +++KN S F++WIP+N+KTAVCDIPPRG ++SAT I N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y+E
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEIISDEHGID-ATGAYHGDSDLQLERINVYYNEASGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQE 427          
BLAST of Tubulin beta chain vs. UniProt/SwissProt
Match: sp|O17449|TBB1_MANSE (Tubulin beta-1 chain OS=Manduca sexta OX=7130 PE=2 SV=1)

HSP 1 Score: 567.385 bits (1461), Expect = 0.000e+0
Identity = 268/429 (62.47%), Postives = 347/429 (80.89%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            MREI++IQ GQCGN IG KFWE+I++EHGI+  TG Y GDSDLQL+RI VY+N  + GKYVPR+++VDL+  T+D + +   G++F+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ +  V  L   MF  +NMMAAC+P +G+YLT+A IFRGRMS KEV+E +L +++KN S F++WIP+N+KTAVCDIPPRG ++SAT I N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y+E
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDP-TGAYHGDSDLQLERINVYYNEASGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLSTPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQE 427          
BLAST of Tubulin beta chain vs. TrEMBL
Match: A0A0X3PFM5 (Tubulin beta chain OS=Schistocephalus solidus OX=70667 GN=TBB4 PE=3 SV=1)

HSP 1 Score: 575.474 bits (1482), Expect = 0.000e+0
Identity = 273/437 (62.47%), Postives = 349/437 (79.86%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEEKDNGDHGE 1373
            MREI++IQ GQCGN IG+KFWE+I++EHGI+  TG Y GDSDLQL+RI VYFN    GKYVPR+V+VDL+  T+D +     G+LF+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL NPT+ DLNHL+  T+SG+TTC RFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ +  V  L   MF  +NMMAAC+P +G+YLT+A IFRGRMS KEV+E +L V++KN S F++WIP+N+KTAVCDIPPRG ++SAT + N+TAIQE F+RV +QF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y++    D GE
Sbjct:    1 MREIVHIQAGQCGNQIGSKFWEVISDEHGIDP-TGAYHGDSDLQLERINVYFNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMTTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTNPTYSDLNHLVSATMSGVTTCFRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALTVAELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFVGNSTAIQELFKRVSDQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDASADDDGE 435          
BLAST of Tubulin beta chain vs. TrEMBL
Match: C1LM62 (Tubulin beta chain OS=Schistosoma japonicum OX=6182 GN=EWB00_008460 PE=2 SV=1)

HSP 1 Score: 574.318 bits (1479), Expect = 0.000e+0
Identity = 275/443 (62.08%), Postives = 353/443 (79.68%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEEKDNGDHGEDDGNPE 1391
            MREI++IQ GQCGN IG+KFWE+I++EHGI+  TG Y GDSDLQL+RI VY+N  + GKYVPR+++VDL+  T+D +     G+LF+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL NPT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ S  V  L   MF  +NMMAAC+P +G+YLT+A IFRGRMS KEV+E +L V++KN S F++WIP+N+KTAVCDIPPRG ++S T I N+TAIQE F+RV EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y++    D GE + + E
Sbjct:    1 MREIVHIQAGQCGNQIGSKFWEVISDEHGIDP-TGTYHGDSDLQLERINVYYNEASGGKYVPRAILVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTNPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-SLTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSVTFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATADDEGEFEEDAE 441          
BLAST of Tubulin beta chain vs. TrEMBL
Match: A0A0P5FPY0 (Tubulin beta chain OS=Daphnia magna OX=35525 PE=3 SV=1)

HSP 1 Score: 573.933 bits (1478), Expect = 0.000e+0
Identity = 274/448 (61.16%), Postives = 360/448 (80.36%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEEKDNGDHG--EDDGNPEADN 1400
            MREI++IQ+GQCGN IG KFWE+I++EHGI    GVY+G+S+LQL+RI+VY+N  + GKYVPR+V+VDL+  T+D + +S  G++F+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ +  V  L   MF  +NMMAAC+P +G+YLT+A+IFRGRMS KEV+E +L +++KN S F++WIP+N+KTAVCDIPPRG +++AT I N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y+E    D    E+D + EADN
Sbjct:   31 MREIVHIQVGQCGNQIGAKFWEIISDEHGIQP-NGVYQGESELQLERIDVYYNEASGGKYVPRAVLVDLEPGTMDSVRSSPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAEGCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALTVPELTQQMFDAKNMMAACDPRHGRYLTVASIFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFSAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATADDEADFEEDPDAEADN 476          
BLAST of Tubulin beta chain vs. TrEMBL
Match: A0A162PE16 (Tubulin beta chain OS=Daphnia magna OX=35525 GN=APZ42_015339 PE=3 SV=1)

HSP 1 Score: 573.548 bits (1477), Expect = 0.000e+0
Identity = 274/448 (61.16%), Postives = 360/448 (80.36%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEEKDNGDHG--EDDGNPEADN 1400
            MREI++IQ+GQCGN IG KFWE+I++EHGI    GVY+G+S+LQL+RI+VY+N  + GKYVPR+V+VDL+  T+D + +S  G++F+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ +  V  L   MF  +NMMAAC+P +G+YLT+A+IFRGRMS KEV+E +L +++KN S F++WIP+N+KTAVCDIPPRG +++AT I N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y+E    D    E+D + EADN
Sbjct:    1 MREIVHIQVGQCGNQIGAKFWEIISDEHGIQP-NGVYQGESELQLERIDVYYNEASGGKYVPRAVLVDLEPGTMDSVRSSPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAEGCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALTVPELTQQMFDAKNMMAACDPRHGRYLTVASIFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFSAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATADDEADFEEDPDAEADN 446          
BLAST of Tubulin beta chain vs. TrEMBL
Match: E9HEX5 (Tubulin beta chain OS=Daphnia pulex OX=6669 GN=DAPPUDRAFT_300845 PE=3 SV=1)

HSP 1 Score: 573.548 bits (1477), Expect = 0.000e+0
Identity = 274/448 (61.16%), Postives = 360/448 (80.36%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEEKDNGDHG--EDDGNPEADN 1400
            MREI++IQ+GQCGN IG KFWE+I++EHGI    GVY+G+S+LQL+RI+VY+N  + GKYVPR+V+VDL+  T+D + +S  G++F+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ +  V  L   MF  +NMMAAC+P +G+YLT+A+IFRGRMS KEV+E +L +++KN S F++WIP+N+KTAVCDIPPRG +++AT I N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y+E    D    E+D + EADN
Sbjct:    1 MREIVHIQVGQCGNQIGAKFWEIISDEHGIQP-NGVYQGESELQLERIDVYYNEASGGKYVPRAVLVDLEPGTMDSVRSSPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAEGCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALTVPELTQQMFDAKNMMAACDPRHGRYLTVASIFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFSAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATADDEADFEEDPDAEADN 446          
BLAST of Tubulin beta chain vs. Ensembl Cavefish
Match: zgc:55461 (tubulin beta-4B chain [Source:NCBI gene;Acc:103024068])

HSP 1 Score: 564.688 bits (1454), Expect = 0.000e+0
Identity = 266/429 (62.00%), Postives = 346/429 (80.65%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            MREI+++Q GQCGN IG KFWE+I++EHGI+  TG Y GDSDLQL+RI VY+N    GKYVPR+V+VDL+  T+D + +   G++F+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ +  V  L   MF  +NMMAAC+P +G+YLT+A +FRGRMS KEV+E +L V++KN S F++WIP+N+KTAVCDIPPRG +++AT I N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y++
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDP-TGAYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Cavefish
Match: TUBB2A (tubulin beta-2A chain [Source:NCBI gene;Acc:103041736])

HSP 1 Score: 561.607 bits (1446), Expect = 0.000e+0
Identity = 265/436 (60.78%), Postives = 349/436 (80.05%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEEKDNGDHG 1370
            MREI++IQ GQCGN IG KFWE+I++EHGI+  TG Y+G+S+LQL+RI VY+N     K+VPR+++VDL+  T+D + +   G++F+PD F+ G S A NN+AKG YT+GAE++D +++++R + E+CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S++PSPK+S+TVVEPYN TL  + L   +DETF IDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ + +V  L   MF  +NMMAAC+P +G+YLT+A IFRGRMS KEV+E +L V++KN S F++WIP+N+KTAVCDIPPRG ++SAT I N+TAIQE FRR+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y++    + G
Sbjct:   78 MREIVHIQAGQCGNQIGAKFWEVISDEHGID-ATGSYQGESELQLERINVYYNEATGSKFVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESENCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETFSIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALSVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLSVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFRRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATADEMG 511          
BLAST of Tubulin beta chain vs. Ensembl Cavefish
Match: TUBB1 (tubulin beta 1 class VI [Source:HGNC Symbol;Acc:HGNC:16257])

HSP 1 Score: 489.574 bits (1259), Expect = 1.565e-169
Identity = 245/429 (57.11%), Postives = 317/429 (73.89%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            MREI+++Q+GQCGN IG+K  E+      I+T                         GKYVPR+++VDL+  T+D +  S IG+LF+PD FI G S A NN+AKG YT+GAE+++++++ +R + E CD LQGFQ  + LGGGTGSG+G+LI+ KIREE+PDRI+++ SI+PSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TT LRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLT+R ++ Y+ +  V  L   MF   NMM AC+P  G+YLT+A IFRGRMSTKEV+E +L ++ KN + F+DWIP N+K AVCDIPPRG ++++T I NNTAIQE FRRV EQF  M+RR AFLHWYTGEGM+  EF EA +N+ DL++EYQ Y++
Sbjct:    1 MREIVHLQIGQCGNQIGSKVTEI----RLIDTC-----------------------GGKYVPRALLVDLEPGTMDSVRGSRIGQLFRPDNFIHGNSGAGNNWAKGHYTEGAELVEQVVDRVRNEAESCDCLQGFQFVHSLGGGTGSGMGTLIINKIREEYPDRIMNSFSIMPSPKVSDTVVEPYNATLSIHQLIENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSLTMSGVTTSLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTARGSQQYR-ALTVPELTQQMFDARNMMTACDPRRGRYLTVAGIFRGRMSTKEVDEQMLAIQQKNSNYFVDWIPHNVKVAVCDIPPRGLKMASTFIGNNTAIQEIFRRVGEQFAMMFRRKAFLHWYTGEGMDEMEFTEAENNLNDLVSEYQQYQD 401          
BLAST of Tubulin beta chain vs. Ensembl Cavefish
Match: ENSAMXT00000019550.2 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000026.1:1405913:1407004:-1 gene:ENSAMXG00000018987.2 transcript:ENSAMXT00000019550.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 442.965 bits (1138), Expect = 2.194e-152
Identity = 211/340 (62.06%), Postives = 274/340 (80.59%), Query Frame = 3
Query:  330 YFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            +   G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ +  V  L   +F  +NMMAAC+P +G+YLT+A +FRGRMS KEV+E +L V++KN S F++WIP+N+KTAVCDIPPRG +++ T I N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y++
Sbjct:    8 HLSAGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 346          
BLAST of Tubulin beta chain vs. Ensembl Cavefish
Match: ENSAMXT00000019223.2 (tubulin alpha-1 chain [Source:NCBI gene;Acc:103031418])

HSP 1 Score: 308.531 bits (789), Expect = 9.131e-99
Identity = 164/436 (37.61%), Postives = 254/436 (58.26%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQR---IEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPP--------RGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQ 1337
            MRE ++I +GQ G   G   WEL   EHG+    GV +  + L   R      +FN+G++G++VPR+VMVDL+ T +D + +    +LF P+  I G   A+NN+A+G YT G EIID ++E +R   + C  LQGF +F+  GGGTGSG  SL++ ++  ++  +     +I P+P++S  VVEPYN  L  +     SD  F +DNEA+Y ICR NL +  P++ +LN LI   VS IT  LRF G LN DL +   N+VP+PR+HF +  ++P+ S + + Y +  +V  + +  F+P N M  C+P +GKY+    ++RG +  K+V   +  ++ K    F+DW P+  K  +   PP           + +  +++N TAI E++ R+  +F  MY + AF+HWY GEGME  EF EA +++  L  +Y+
Sbjct:    1 MRECISIHVGQAGIQTGNACWELFCLEHGVGP-DGVMQDVAALSNSRQDPFNTFFNTGSSGRHVPRAVMVDLEPTVVDEVRSGIYRQLFHPEQLISGKEDAANNYARGHYTVGKEIIDVVLERVRKLTDQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPSYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYSPIIS-TEKAYHEQLSVAEITSACFEPSNQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIANIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYE 434          
BLAST of Tubulin beta chain vs. Ensembl Sea Lamprey
Match: tubb2 (tubulin, beta 2A class IIa [Source:ZFIN;Acc:ZDB-GENE-050522-384])

HSP 1 Score: 464.151 bits (1193), Expect = 3.111e-161
Identity = 225/376 (59.84%), Postives = 300/376 (79.79%), Query Frame = 3
Query:  117 KFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTI-SIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRR 1241
            +FWE+I++EHGI+  TG Y GDSDLQL+RI VY+N  + GKYVPR+++VDL+  T+D + +   G++F+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+  +++ T+ +  PSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ +  V  L   MF  +NMMAAC+P +G+YLT+A IFRGRMS KEV+E +L V++KN S F++WIP+N+KTAVCDIPPRG ++SAT I N+TAIQE F+R+ EQF AM+RR
Sbjct:    1 QFWEVISDEHGIDP-TGSYHGDSDLQLERINVYYNEASGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYIIQVLYTVVASGPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRR 374          
BLAST of Tubulin beta chain vs. Ensembl Sea Lamprey
Match: TUBA3C (tubulin alpha 3c [Source:HGNC Symbol;Acc:HGNC:12408])

HSP 1 Score: 248.054 bits (632), Expect = 1.754e-77
Identity = 137/359 (38.16%), Postives = 202/359 (56.27%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGIN---------TVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSN 1112
            MRE ++I +GQ G  IG   WEL   EHGI          T+ G   GD          +F+    GK+VPR+V VDL+ T +D +      +LF P+  I G   A+NN+A+G YT G EI+D +++ IR   + C  LQGF +F+  GGGTGSG  SL++ ++  ++  +     +I P+P++S  VVEPYN  L  +     SD  F +DNEA+Y ICR NL +  PT+ +LN LI   VS IT  LRF G LN DL +   N+VP+PR+HF +  +AP+ S + + Y +  +V  +    F+P N M  C+P +GKY+    ++RG +  K+V   +  ++ K    F+DW P+ 
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGG---GDDSFN-----TFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVIS-AEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTG 350          
BLAST of Tubulin beta chain vs. Ensembl Sea Lamprey
Match: tubg1 (tubulin, gamma 1 [Source:ZFIN;Acc:ZDB-GENE-040426-836])

HSP 1 Score: 233.032 bits (593), Expect = 1.409e-70
Identity = 138/442 (31.22%), Postives = 238/442 (53.85%), Query Frame = 3
Query:   66 REILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPD--YFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSP-KISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACN---PANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPP---RGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGM---EASEFIEAHDNMVDLLNEYQMYEEKD 1355
            REI+ +Q+GQCGN IG +FW+ +  EHGI+   G+ E  +     R +V+F   ++  Y+PR+V++DL+   ++ I+NS+   L+ P+  Y       A NN+A G ++ G +I +EI ++I  + +  D L+   +         S      L K    +P ++I T S+ P+  ++S+ VV+PYN  L    L+  +D    +DN AL RI  + L + NP+F  +N L+   +S  TT LR+PG +N DL  L  +++P PRLHF +  + PLT+  +        V  ++  + +P+N+M +       N  Y+ I  I +G +   +V + + R+R++ L+ FI W P++I+ A+    P      R+S  ++AN+T+I   F R  +Q+  + +R AFL  +  E M   + SEF ++ + +  L++EYQ     D
Sbjct:    3 REIITLQLGQCGNQIGFEFWKQLCAEHGISP-DGILEDFATEGTDRKDVFFYQADDEHYIPRAVLLDLEPRVINSIVNSSYANLYNPENIYLSEHGGGAGNNWASG-HSQGEKIHEEIFDIIDREADGSDSLEKCPL-----TRRLSATNWNELCKYFCRFPKKLIQTYSVFPNQDEMSDVVVQPYNSLLTLKRLTQNADCVVVLDNTALNRIATDRLHIQNPSFSQINQLVSTIMSASTTTLRYPGYMNNDLIGLIASLIPTPRLHFLMTGYTPLTTDQSVASVRKTTVLDVMRRLLQPKNVMVSTGRDRQTNHCYIAILNIIQGEVDPTQVHKSLQRIRERKLANFIPWGPASIQVALSRKSPYLTSAHRVSGLMMANHTSISSLFDRTCKQYDKLRKREAFLDQFRKEDMFKEDLSEFDDSREVVQQLIDEYQAATRPD 437          
BLAST of Tubulin beta chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001368.1 (pep scaffold:Pmarinus_7.0:GL476337:866089:875956:-1 gene:ENSPMAG00000001230.1 transcript:ENSPMAT00000001368.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 209.92 bits (533), Expect = 1.670e-61
Identity = 135/458 (29.48%), Postives = 229/458 (50.00%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNN-----------GKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRIC----------------------RNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTG-EGMEASEFIEAHDNMVDLLNEY 1334
            M + + +Q+GQCGN IG +FW+L   EH      G+Y+       + ++    +G                  R+V++D++   ++ +L   + ++F     I   S + NN+A G    GA+  ++I+E +R+  E CD LQ F + + +GGGTGSGLG+ +L  + +E+PD     +++ PS +  + V  PYN  L    L+  +D    I+N+AL  I                          LK     F+ +N+++ N +  +T+  RF G LN DL ++ +N+VPFPRLH+ + +  PL + ++        ++ + ++ F  EN +   +P    YL  A + RG +   +V   + R++      F+DW     KT +C +PP G+  S   +ANNT ++ +F  + E+F  +YRR A LH YT  EGME S F EA  ++  ++ EY
Sbjct:    1 MAQSVVVQVGQCGNQIGCRFWDLALREHAAVNKKGIYDEALSSFFRNVDTRPGTGEEVDIPVDSRSTIRSLKARAVLIDMEEGVVNELLQGPLRDVFDQKQLITDVSGSGNNWAVGNKVYGAQFREKILERLRVTAEHCDSLQCFFLIHSMGGGTGSGLGTFVLRLLEDEFPDVHRFVMALYPSAE-DDVVTSPYNSVLAMRELTEHADCVLPIENQALVDIVNKINHAAALGKSVRSQTTLSSSHGCLKGQEKPFDTMNNIVANLMLNMTSSSRFEGSLNVDLNEITMNLVPFPRLHYLVSSLTPLYALADVALPPR-RLDQMFSDAFSKENQLLRVDPRRSLYLACALMVRGNVQLSDVRRNIERLKPS--LHFVDWNEEGWKTGLCSVPPVGYPHSLLALANNTCVKHTFADIQERFARLYRRKAHLHHYTDVEGMELSCFTEAASSLASVIEEY 454          
BLAST of Tubulin beta chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001139.1 (pep scaffold:Pmarinus_7.0:GL478075:30403:34872:1 gene:ENSPMAG00000001018.1 transcript:ENSPMAT00000001139.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 201.83 bits (512), Expect = 8.024e-60
Identity = 115/354 (32.49%), Postives = 188/354 (53.11%), Query Frame = 3
Query:   66 REILNIQMGQCGNNIGTKFWELITNEHGI--NTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDY---ILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSN 1112
            R+ ++I +GQ G  +G   WEL   EHGI  +     +   + +       +F+   +GK+VPR++ +DL+ + +     +    +G   +    + G    S+   +G    G +I   ++   R Q E C  LQGF +F+  GGGTGSG  SL++ ++  ++  +      I P+P++S  VVEPYN  L  +     SD +F +DNEA+Y ICR  L +  P++ +LN LI   VS IT  LRF G LN DL +   N+VP+PR+HF +  +AP+ S + + Y +  +V  +    F+P N M  C+P NGKY+    ++RG +  K+V   +  ++ +    F+DW P+ 
Sbjct:    3 RDCISIHIGQAGVQMGNACWELYCLEHGIKPDGHLATHPDVTSVVDSSFNTFFSETGSGKHVPRAIFIDLEPSVIGEPCGLRTLTVGGXLRMSMMLAGREVRSSRIWRGQKGGGRKICSRVLRRNRPQSERCTGLQGFLLFHSFGGGTGSGFTSLLMERLSADYGKKSKLEFCIYPAPQVSTAVVEPYNAILTTHTTLEHSDCSFMVDNEAIYDICRKKLDIDFPSYFNLNRLIAQMVSSITASLRFDGALNVDLNEFQTNLVPYPRIHFPLTTYAPVIS-AEKAYHEQLSVAEITNACFEPSNQMVKCDPRNGKYMACCLLYRGDIVPKDVNTAIAIIKSRRAVRFVDWCPTG 355          
BLAST of Tubulin beta chain vs. Ensembl Yeast
Match: TUB2 (Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria [Source:SGD;Acc:S000001857])

HSP 1 Score: 505.368 bits (1300), Expect = 2.214e-176
Identity = 247/441 (56.01%), Postives = 334/441 (75.74%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEEKDNGDHGEDDGN 1385
            MREI++I  GQCGN IG  FWE I  EHG++   G Y G  D+Q +R+ VYFN  ++GK+VPRS+ VDL+  T+D + NS IG LF+PD +I G SSA N +AKG YT+GAE++D +M++IR + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDR+++T S++PSPK S+TVVEPYN TL  + L   SDETFCIDNEALY IC+  LKL  P++ DLN+L+ + +SG+TT LR+PGQLN+DLRKLAVN+VPFPRLHFF+  +APLT+  +++++ S  V  L   MF  +NMMAA +P NG+YLT+A  FRG++S KEVE+ + +V+ KN   F++WIP+N++TAVC + P+G  ++AT IAN+T+IQE F+RV +QF AM++R AFLHWYT EGM+  EF EA  NM DL++EYQ Y+E    D  E D N
Sbjct:    1 MREIIHISTGQCGNQIGAAFWETICGEHGLD-FNGTYHGHDDIQKERLNVYFNEASSGKWVPRSINVDLEPGTIDAVRNSAIGNLFRPDNYIFGQSSAGNVWAKGHYTEGAELVDSVMDVIRREAEGCDSLQGFQITHSLGGGTGSGMGTLLISKIREEFPDRMMATFSVLPSPKTSDTVVEPYNATLSVHQLVEHSDETFCIDNEALYDICQRTLKLNQPSYGDLNNLVSSVMSGVTTSLRYPGQLNSDLRKLAVNLVPFPRLHFFMVGYAPLTAIGSQSFR-SLTVPELTQQMFDAKNMMAAADPRNGRYLTVAAFFRGKVSVKEVEDEMHKVQSKNSDYFVEWIPNNVQTAVCSVAPQGLDMAATFIANSTSIQELFKRVGDQFSAMFKRKAFLHWYTSEGMDELEFSEAESNMNDLVSEYQQYQEATVEDDEEVDEN 439          
BLAST of Tubulin beta chain vs. Ensembl Yeast
Match: TUB3 (Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication [Source:SGD;Acc:S000004593])

HSP 1 Score: 312.383 bits (799), Expect = 4.227e-101
Identity = 167/434 (38.48%), Postives = 257/434 (59.22%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQ--LQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRIS--------ATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEY 1334
            MRE+++I +GQ G  IG   WEL + EHGI     + +G S  +   +    +F+    GK+VPR++ VDL+   +D +      ELF P+  I G   A+NN+A+G YT G EI+DE+ E IR   + CD LQGF   + LGGGTGSGLGSL+L  +  E+  +     ++ P+P++S +VVEPYN  L  +     +D TF +DNEA+Y IC+ NL +  P+F +LN LI   +S +T  LRF G LN DL +   N+VP+PR+HF + ++AP+ S+    + +S +V+ +    F+P N M  C+P  GKY+    ++RG + T++V+  V +V++K     +DW P+  K  +C  PP     S          +++N TAI ++++R+ ++F  MY + AF+HWY GEGME  EF EA +++  L  +Y
Sbjct:    1 MREVISINVGQAGCQIGNACWELYSLEHGIKEDGHLEDGLSKPKGGEEGFSTFFHETGYGKFVPRAIYVDLEPNVIDEVRTGRFKELFHPEQLINGKEDAANNYARGHYTVGREIVDEVEERIRKMADQCDGLQGFLFTHSLGGGTGSGLGSLLLENLSYEYGKKSKLEFAVYPAPQLSTSVVEPYNTVLTTHTTLEHADCTFMVDNEAIYDICKRNLGISRPSFSNLNGLIAQVISSVTASLRFDGSLNVDLNEFQTNLVPYPRIHFPLVSYAPILSKKRATH-ESNSVSEITNACFEPGNQMVKCDPTKGKYMANCLLYRGDVVTRDVQRAVEQVKNKKTVQMVDWCPTGFKIGICYEPPSVIPSSELANVDRAVCMLSNTTAIADAWKRIDQKFDLMYAKRAFVHWYVGEGMEEGEFTEAREDLAALERDY 433          
BLAST of Tubulin beta chain vs. Ensembl Yeast
Match: TUB1 (Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication [Source:SGD;Acc:S000004550])

HSP 1 Score: 311.612 bits (797), Expect = 8.450e-101
Identity = 164/434 (37.79%), Postives = 263/434 (60.60%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQ--LQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRG--------FRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEY 1334
            MRE+++I +GQ G  IG   WEL + EHGI     + +G S  +   +    +F+    GK+VPR++ VDL+   +D + N    +LF P+  I G   A+NN+A+G YT G EI+ ++++ IR   + CD LQGF   + LGGGTGSGLGSL+L ++  E+  +     ++ P+P++S +VVEPYN  L  +     +D TF +DNEA+Y +C+ NL +P P+F +LN+LI   VS +T  LRF G LN DL +   N+VP+PR+HF + +++P+ S+S + + +S +V+ +    F+P N M  C+P +GKY+    ++RG + T++V+  V +V++K     +DW P+  K  +C  PP             +  +++N T+I E+++R+  +F  MY + AF+HWY GEGME  EF EA +++  L  +Y
Sbjct:    1 MREVISINVGQAGCQIGNACWELYSLEHGIKPDGHLEDGLSKPKGGEEGFSTFFHETGYGKFVPRAIYVDLEPNVIDEVRNGPYKDLFHPEQLISGKEDAANNYARGHYTVGREILGDVLDRIRKLADQCDGLQGFLFTHSLGGGTGSGLGSLLLEELSAEYGKKSKLEFAVYPAPQVSTSVVEPYNTVLTTHTTLEHADCTFMVDNEAIYDMCKRNLDIPRPSFANLNNLIAQVVSSVTASLRFDGSLNVDLNEFQTNLVPYPRIHFPLVSYSPVLSKS-KAFHESNSVSEITNACFEPGNQMVKCDPRDGKYMATCLLYRGDVVTRDVQRAVEQVKNKKTVQLVDWCPTGFKIGICYEPPTATPNSQLATVDRAVCMLSNTTSIAEAWKRIDRKFDLMYAKRAFVHWYVGEGMEEGEFTEAREDLAALERDY 433          
BLAST of Tubulin beta chain vs. Ensembl Yeast
Match: TUB4 (Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress [Source:SGD;Acc:S000004202])

HSP 1 Score: 146.362 bits (368), Expect = 1.513e-38
Identity = 114/444 (25.68%), Postives = 211/444 (47.52%), Query Frame = 3
Query:   69 EILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQR-IEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQP--DYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSR--SNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNI------KTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWY-TGEGMEA-----SEFIEAHDNMVDLLNEYQMYEE 1349
            EI+ +Q GQCGN++G   W  +  EH I T       DS  +     + +F   +  K+ PR++M+D + + +  + N+  G  F P   +     +SA N++A G Y  G    D+I+  I  +++  D  +GFQ+ + + GGTGSGLGS +L  + + +P +I++T S+ P+ + SE VV+ YN  L    L   SD T   DN +L  I     + PN   +  N LI   +S +T  +RFP  + + +  +   ++P P LHF  P+F P TS    ++      +   ++ ++  P N + +    N  Y  +     G +  +++   + +++ +    F  W  S +      ++    + P    +S  +++N + +   F      F  ++ + AFL+ Y  G+  ++      EF E+ + +  L+ +Y   E+
Sbjct:    4 EIITLQAGQCGNHVGKFLWSQLAKEHAIGTDGLSQLPDSSTERDDDTKPFFRENSRNKFTPRAIMMDSEPSVIADVENTFRG-FFDPRNTWVASDGASAGNSWANG-YDIGTRNQDDILNKIDKEIDSTDNFEGFQLLHSVAGGTGSGLGSNLLEALCDRYPKKILTTYSVFPA-RSSEVVVQSYNTILALRRLIEDSDATVVFDNASLLNISGKVFRNPNIDLQHTNQLISTIISSVTNSIRFPSYMYSSMSSIYSTLIPSPELHFLSPSFTPFTSDYIHDDIAHKGHSSYDVMLDLLDPSNSLVSTAMNNPTYFNVYNTIIGNVEPRQISRAMTKLQQR--IKFPSWSSSAMHVNIGRRSPYLPLQPNENEVSGMMLSNMSTVVNVFENACNTFDKVFAKGAFLNNYNVGDLFQSMQNVQDEFAESREVVQSLMEDYVAAEQ 442          
BLAST of Tubulin beta chain vs. Ensembl Nematostella
Match: EDO35958 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJX9])

HSP 1 Score: 564.303 bits (1453), Expect = 0.000e+0
Identity = 267/429 (62.24%), Postives = 346/429 (80.65%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            MREI+++Q GQCGN IG KFWE+I++EHGI+  TG Y GDSDLQL+RI VY+N    GKYVPRSV++DL+  T+D + +   G++F+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD +QGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T SIVPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ +  V  L   MF  +NMMAAC+P +G+YLT+A +FRGRMS KEV+E +L V++KN S F++WIP+N+KTAVCDIPPRG ++SAT I N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y++
Sbjct:    1 MREIVSLQAGQCGNQIGAKFWEVISDEHGIDP-TGTYHGDSDLQLERINVYYNEATGGKYVPRSVLIDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELMDSVLDVVRKEAESCDCIQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSIVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Nematostella
Match: EDO39945 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S8I3])

HSP 1 Score: 563.533 bits (1451), Expect = 0.000e+0
Identity = 266/429 (62.00%), Postives = 345/429 (80.42%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            MREI++ Q GQCGN IG KFWE+I++EHGI+  TG Y GDSDLQL+RI VY+N    GKYVPR+++VDL+  T+D + +   G++F+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ +  V  L   MF  +NMMAAC+P +G+YLT+A +FRGRMS KEV+E +L V++KN S F++WIP+N+KTAVCDIPPRG ++SAT I N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y++
Sbjct:    1 MREIVHCQAGQCGNQIGAKFWEVISDEHGIDP-TGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Nematostella
Match: EDO42890 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S027])

HSP 1 Score: 550.051 bits (1416), Expect = 0.000e+0
Identity = 262/429 (61.07%), Postives = 339/429 (79.02%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            MREI+++Q GQCGN IG KFW    NEHGI+  TG Y GDSDLQL+RI VY+N    GKYVPR+V+VDL+  T+D + +   G++F+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD +QGFQ+ + LGGGTGSG+ +L++ KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRL FF+P FAPLTSR ++ Y+ +  V  L   MF  +NMMAAC+P +G+YLT+A +FRGRMS KE++E +L V++KN S F++WIP+N+KTAVCDIPPRG ++SAT I N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y++
Sbjct:    1 MREIVHLQAGQCGNQIGAKFW----NEHGIDP-TGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCIQGFQLTHSLGGGTGSGMVTLLILKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLPFFMPGFAPLTSRGSQQYR-ALTVPELTQQMFDAKNMMAACDPLHGRYLTVAAMFRGRMSMKELDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 423          
BLAST of Tubulin beta chain vs. Ensembl Nematostella
Match: EDO49518 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGG3])

HSP 1 Score: 509.99 bits (1312), Expect = 8.522e-178
Identity = 254/447 (56.82%), Postives = 328/447 (73.38%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYE--GDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEEKDNGDHGE--DDGNPE 1391
            MREI+NIQ+GQCGN IGT+FW  I  EHGI+  TG      D+  +  RI+VYF+ GN G+YVPRS++VDL+  T+D +    +G +F+PD F+   S A+NN+AKG Y++GAE+ID I +++R + E+CD +QG Q+   LGGGTGSGLG+LIL+KIREE+PDRI+S  S+VPSPK+SETVVEPYN TL  + L  + DE F  DNEALY IC   LKL  P + DLN L+   + G+T  LRFPGQLN DLRKLAVNMVPFPRLHFF+P FAPL SR+N+ Y++  +V  L   MF   NMM AC+P +G+YLT+A IFRG+MS +EV+E +L  ++KN   F++WIP+N+KT VCDI P+G +++AT + NNTAIQE F+RV EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL+ EYQ Y+E +    GE  D+  PE
Sbjct:    1 MREIINIQVGQCGNQIGTEFWRDIAEEHGIDP-TGAKSLIEDTHGRFDRIDVYFHEGNGGRYVPRSILVDLEPGTMDAVRGGPLGNMFRPDNFVNSQSGAANNWAKGHYSEGAELIDNIFDVLRKEAENCDCMQGVQLIQALGGGTGSGLGTLILSKIREEYPDRILSAFSVVPSPKVSETVVEPYNATLSVHQLVEVVDEDFMFDNEALYDICCRTLKLAKPNYADLNQLVSMAMCGLTASLRFPGQLNGDLRKLAVNMVPFPRLHFFMPGFAPLASRANQQYQN-VSVEELTQQMFDARNMMTACDPRHGRYLTVAAIFRGKMSMREVDEQMLNTQNKNSPFFVEWIPNNVKTTVCDIAPKGLKMAATFVGNNTAIQELFKRVGEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMHDLIAEYQQYQEAEADIEGEVSDEELPE 445          
BLAST of Tubulin beta chain vs. Ensembl Nematostella
Match: EDO43980 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RX70])

HSP 1 Score: 489.96 bits (1260), Expect = 5.846e-170
Identity = 240/434 (55.30%), Postives = 323/434 (74.42%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEEKDNGD 1364
            MR+I+++Q+GQCGN+I +KFWE+I  EHGI+         S +Q  R+ VYF+   +G++VPRS  +DL+  ++D +  S  G LF P+  I G +SA+NN+AKGFYT+GAE++D  M+ IR   E+CD +QGFQ+ + LGGGTGSGLGSL+L+KIREE+PDR++S+  ++PSP +SETV EPYN TL  + L  ++D   CIDNEALY IC N LK   PT+ +LN LI  T+SG+TT LRFPGQLNADLRKLAVNMVPFPRLHFFIP+ APL S  NE Y+ +++V  L   MF P+ MM AC+P  G+YLT+A +FRG+M+ ++VEE +  ++D + S F++WIP+N+KTAVCD+PP G + +AT + N+TAIQE+FRR  EQF AM+RR AFLHWYT EGM+  EF EA  N+ DL++EYQ YE+  + D
Sbjct:    1 MRDIVHLQVGQCGNHISSKFWEVIAEEHGIDPDGQCTAVQSKIQTDRLNVYFDERLDGRFVPRSACIDLEPGSIDALRASRYGRLFNPECCISGQNSAANNWAKGFYTEGAELMDSGMDCIRKLAENCDTVQGFQIAHSLGGGTGSGLGSLLLSKIREEYPDRLVSSFCVLPSPLVSETVTEPYNATLSFHQLVDVADAAICIDNEALYNICYNVLKRETPTYSELNSLIATTMSGVTTSLRFPGQLNADLRKLAVNMVPFPRLHFFIPSHAPLISSRNEEYR-TYHVTELTEKMFGPQGMMVACDPRQGRYLTVAAMFRGKMAMRDVEESISCMQDSHSSSFVEWIPNNMKTAVCDVPPVGLKNAATFLYNSTAIQEAFRRFTEQFAAMFRRRAFLHWYTTEGMDPLEFTEADSNLNDLISEYQQYEDAKSDD 433          
BLAST of Tubulin beta chain vs. Ensembl Medaka
Match: tubb2 (tubulin beta-2B chain [Source:NCBI gene;Acc:101162245])

HSP 1 Score: 565.459 bits (1456), Expect = 0.000e+0
Identity = 268/429 (62.47%), Postives = 349/429 (81.35%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            MREI++IQ GQCGN IG KFWE+I++EHGI+  TG Y+GDSDLQL+RI VY+N  +  KYVPR+++VDL+  T+D + +   G+LF+PD F+ G S A NN+AKG YT+GAE++D +++++R + E+CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S++PSPK+S+TVVEPYN TL  + L   +DETFCIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ + +V  L   MF  +NMMAAC+P +G+YLT+A IFRGRMS +EV+E +L V++KN S F++WIP+N+KTAVCDIPPRG ++SAT I N+TAIQE FRR+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y++
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDP-TGSYQGDSDLQLERINVYYNEASGSKYVPRAILVDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDAVLDVVRKESENCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALSVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMREVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFRRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Medaka
Match: tubb5 (tubulin beta chain [Source:NCBI gene;Acc:101157969])

HSP 1 Score: 559.681 bits (1441), Expect = 0.000e+0
Identity = 264/429 (61.54%), Postives = 344/429 (80.19%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            MREI++IQ GQCGN IG KFWE+I++EHGI+  TG Y GDSDLQL RI VY+N    GKYVPR+++VDL+  T+D + +   G++F+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DET+CIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ +  V  L   +F  +NMMAAC+P +G+YLT+A +FRGRMS KEV+E +L V++KN S F++WIP+N+KTAVCDIPPRG +++ T I N+TAIQE F+R+ EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y++
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDP-TGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Medaka
Match: ENSORLT00000045809.1 (tubulin beta-6 chain [Source:NCBI gene;Acc:101166121])

HSP 1 Score: 558.14 bits (1437), Expect = 0.000e+0
Identity = 268/429 (62.47%), Postives = 340/429 (79.25%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            MREI+++Q+GQCGN IG+KFWE+I+ EHGIN  TG+YEGDS LQL+RI VYF+    GKYVPR+++VDL+  T+D I  S IG LF+PD FI G S A NN+AKG YT+GAE+++++++ +R + E CD LQGFQ+ + LGGGTGSG+G+LI+ KI+EE+PDRI++T SI+PSPK+S+TVVEPYN TL  + L   +D TFCIDNEALY IC   LKL  PT+ DLNHL+C T+SG+TT LRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLT+R ++ Y+ +  V  L   MF   NMM AC+P  G+YLT+A IFRG+MSTKEV+E +L ++ KN + F+DWIP N+K AVCDIPP+G R+++T I NNTAIQE FRRV EQF  M+RR AFLHWYTGEGM+  EF EA  N+ DL++EYQ Y+E
Sbjct:    1 MREIVHVQIGQCGNQIGSKFWEVISEEHGIN-ATGIYEGDSTLQLERINVYFSEAYGGKYVPRALLVDLEPGTMDSIRGSRIGALFRPDNFIHGNSGAGNNWAKGHYTEGAELVEQVVDRVRHESEGCDCLQGFQLVHSLGGGTGSGMGTLIINKIKEEFPDRIMNTFSIMPSPKVSDTVVEPYNATLSVHQLLENADATFCIDNEALYDICFRTLKLTTPTYGDLNHLVCMTMSGVTTSLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTARGSQQYR-ALTVPELTQQMFDARNMMTACDPRRGRYLTVAGIFRGKMSTKEVDEQMLAIQQKNSTYFVDWIPHNVKVAVCDIPPKGLRMASTFIGNNTAIQEIFRRVGEQFSLMFRRKAFLHWYTGEGMDEMEFTEAESNLNDLVSEYQQYQE 427          
BLAST of Tubulin beta chain vs. Ensembl Medaka
Match: tuba1c (tubulin alpha-1B chain-like [Source:NCBI gene;Acc:101167538])

HSP 1 Score: 306.605 bits (784), Expect = 5.298e-98
Identity = 168/435 (38.62%), Postives = 245/435 (56.32%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQL--QRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPP--------RGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQ 1337
            MRE ++I +GQ G  IG   WEL   EHGI    G    D  L         +F+    GK+VPR+V VDL+ T +D +      +LF P+  I G   A+NN+A+G YT G EIID +++ IR   + C  LQGF +F+  GGGTGSG  SL++ ++  ++  +     SI P+P++S  VVEPYN  L  +     SD  F +DNEA+Y ICR NL +  PT+ +LN LI   VS IT  LRF G LN DL +   N+VP+PR+HF +  +AP+ S + + Y +  +V  +    F+P N M  C+P +GKY+    +FRG +  K+V   +  ++ K    F+DW P+  K  +   PP           + +  +++N TAI E++ R+  +F  MY + AF+HWY GEGME  EF EA ++M  L  +Y+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQP-DGQMPSDKTLGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVIS-AEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLFRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYE 433          
BLAST of Tubulin beta chain vs. Ensembl Medaka
Match: ENSORLT00000033163.1 (tubulin alpha-1A chain [Source:NCBI gene;Acc:105354455])

HSP 1 Score: 305.064 bits (780), Expect = 2.528e-97
Identity = 168/442 (38.01%), Postives = 247/442 (55.88%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGIN---------TVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPP--------RGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQ 1337
            MRE ++I +GQ G  IG   WEL   EHGI          T+ G   GD          +F+    GK+VPR+V VDL+ T +D +      +LF P+  I G   A+NN+A+G YT G EIID +++ IR   + C  LQGF +F+  GGGTGSG  SL++ ++  ++  +     SI P+P++S  VVEPYN  L  +     SD  F +DNEA+Y ICR NL +  PT+ +LN LI   VS IT  LRF G LN DL +   N+VP+PR+HF +  +AP+ S + + Y +  +V  +    F+P N M  C+P +GKY+    ++RG +  K+V   +  ++ K    F+DW P+  K  +   PP           + +  +++N TAI E++ R+  +F  MY + AF+HWY GEGME  EF EA ++M  L  +Y+
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGG---GDDSFN-----TFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNAILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVIS-AEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYE 433          
BLAST of Tubulin beta chain vs. Planmine SMEST
Match: SMESG000057129.1 (SMESG000057129.1)

HSP 1 Score: 1016.14 bits (2626), Expect = 0.000e+0
Identity = 518/518 (100.00%), Postives = 518/518 (100.00%), Query Frame = 3
Query:   87 MGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEEKDNGDHGEDDGNPEADNISKVTKGKKTRDXXXXXXXXXXXXTVTKSAVSTPEVVNSKSSKSNAQSDRQNELRPKDSWISNVSTEQSLTPKNILKVNY 1640
            MGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNGLGGGTGSGLGSLILTKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEEKDNGDHGEDDGNPEADNISKVTKGKKTRDNSKKSSANSQSKTVTKSAVSTPEVVNSKSSKSNAQSDRQNELRPKDSWISNVSTEQSLTPKNILKVNY
Sbjct:    1 MGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNGLGGGTGSGLGSLILTKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEEKDNGDHGEDDGNPEADNISKVTKGKKTRDNSKKSSANSQSKTVTKSAVSTPEVVNSKSSKSNAQSDRQNELRPKDSWISNVSTEQSLTPKNILKVNY 518          
BLAST of Tubulin beta chain vs. Planmine SMEST
Match: SMESG000047291.1 (SMESG000047291.1)

HSP 1 Score: 570.852 bits (1470), Expect = 0.000e+0
Identity = 271/429 (63.17%), Postives = 347/429 (80.89%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            MREI+++Q GQCGN IG+KFWE+I++EHGI+  TG Y GDSDLQL+RI VYFN    GKYVPR+V++DL+  T+D + +   G+LF+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DETFCIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFFIP FAPLTSR ++ Y+ +  V  L   MF  +NMMAAC+P +G+YLT+A +FRGRMS KEV+E +L V++KN S F++WIP+N+KTAVCDIPPRG ++SAT I N+TAIQE F+RV EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y++
Sbjct:    1 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDP-TGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYR-ALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Planmine SMEST
Match: SMESG000044888.1 (SMESG000044888.1)

HSP 1 Score: 570.852 bits (1470), Expect = 0.000e+0
Identity = 271/429 (63.17%), Postives = 347/429 (80.89%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            MREI+++Q GQCGN IG+KFWE+I++EHGI+  TG Y GDSDLQL+RI VYFN    GKYVPR+V++DL+  T+D + +   G+LF+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DETFCIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFFIP FAPLTSR ++ Y+ +  V  L   MF  +NMMAAC+P +G+YLT+A +FRGRMS KEV+E +L V++KN S F++WIP+N+KTAVCDIPPRG ++SAT I N+TAIQE F+RV EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y++
Sbjct:    1 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDP-TGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYR-ALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Planmine SMEST
Match: SMESG000050810.1 (SMESG000050810.1)

HSP 1 Score: 569.696 bits (1467), Expect = 0.000e+0
Identity = 273/429 (63.64%), Postives = 346/429 (80.65%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            MREI+++Q GQCGN IG+KFWE+I++EHGI+  TG Y GDSDLQL+RI VY+N    GKYVPR+V+VDL+  T+D +     G+LF+PD FI G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DETFCIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ +  V  L   MF  +NMMAAC+P +G+YLT+A IFRGRMS KEV+E +L V++KN S F++WIP+NIKTAVCDIPPRG ++SAT I N+TAIQE F+RV EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y++
Sbjct:    1 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDP-TGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Planmine SMEST
Match: SMESG000047284.1 (SMESG000047284.1)

HSP 1 Score: 568.54 bits (1464), Expect = 0.000e+0
Identity = 271/429 (63.17%), Postives = 346/429 (80.65%), Query Frame = 3
Query:   63 MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEVYFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASNNFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNXXXXXXXXXXXXXXXXKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDETFCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPENMMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSNIKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWYTGEGMEASEFIEAHDNMVDLLNEYQMYEE 1349
            MREI+++Q GQCGN IG+KFWE+I++EHGI+  TG Y GDSDLQL+RI VY+N    GKYVPR+V+VDL+  T+D +     G+LF+PD F+ G S A NN+AKG YT+GAE++D +++++R + E CD LQGFQ+ + LGGGTGSG+G+L+++KIREE+PDRI++T S+VPSPK+S+TVVEPYN TL  + L   +DETFCIDNEALY IC   LKL  PT+ DLNHL+  T+SG+TTCLRFPGQLNADLRKLAVNMVPFPRLHFF+P FAPLTSR ++ Y+ +  V  L   MF  +NMMAAC+P +G+YLT+A +FRGRMS KEV+E +L V++KN S F++WIP+NIKTAVCDIPPRG ++SAT I N+TAIQE F+RV EQF AM+RR AFLHWYTGEGM+  EF EA  NM DL++EYQ Y++
Sbjct:    1 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDP-TGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR-ALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
The following BLAST results are available for this feature:
BLAST of Tubulin beta chain vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TUBB4B0.000e+062.24tubulin beta 4B class IVb [Source:HGNC Symbol;Acc:... [more]
TUBB0.000e+061.54tubulin beta class I [Source:HGNC Symbol;Acc:HGNC:... [more]
TUBB0.000e+061.54tubulin beta class I [Source:HGNC Symbol;Acc:HGNC:... [more]
TUBB4A0.000e+061.77tubulin beta 4A class IVa [Source:HGNC Symbol;Acc:... [more]
TUBB0.000e+061.54tubulin beta class I [Source:HGNC Symbol;Acc:HGNC:... [more]
back to top
BLAST of Tubulin beta chain vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tbb-40.000e+062.41Tubulin beta-4 chain [Source:UniProtKB/Swiss-Prot... [more]
ben-10.000e+060.91Tubulin beta chain [Source:UniProtKB/TrEMBL;Acc:Q... [more]
tbb-10.000e+060.59Tubulin beta chain [Source:UniProtKB/TrEMBL;Acc:O... [more]
tbb-10.000e+060.59Tubulin beta chain [Source:UniProtKB/TrEMBL;Acc:O... [more]
tbb-20.000e+060.00Tubulin beta-2 chain [Source:UniProtKB/Swiss-Prot... [more]
back to top
BLAST of Tubulin beta chain vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
betaTub56D0.000e+062.70gene:FBgn0284243 transcript:FBtr0086536[more]
betaTub85D0.000e+062.24gene:FBgn0003889 transcript:FBtr0082046[more]
betaTub60D0.000e+059.15gene:FBgn0003888 transcript:FBtr0343443[more]
betaTub60D0.000e+059.02gene:FBgn0003888 transcript:FBtr0072270[more]
betaTub97EF0.000e+060.36gene:FBgn0003890 transcript:FBtr0300611[more]
back to top
BLAST of Tubulin beta chain vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
zgc:1534260.000e+062.00zgc:153426 [Source:ZFIN;Acc:ZDB-GENE-060929-220][more]
zgc:658940.000e+062.47zgc:65894 [Source:ZFIN;Acc:ZDB-GENE-030131-7741][more]
zgc:554610.000e+062.00zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879][more]
zgc:554610.000e+062.00zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879][more]
zgc:554610.000e+062.00zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879][more]
back to top
BLAST of Tubulin beta chain vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ubxn110.000e+062.47UBX domain protein 11 [Source:Xenbase;Acc:XB-GENE-... [more]
reps20.000e+062.24RALBP1 associated Eps domain containing 2 [Source:... [more]
tubb0.000e+061.54tubulin beta class I [Source:NCBI gene;Acc:448742][more]
znf3300.000e+061.77zinc finger protein 330 [Source:Xenbase;Acc:XB-GEN... [more]
tubb30.000e+060.18tubulin beta 3 class III [Source:NCBI gene;Acc:395... [more]
back to top
BLAST of Tubulin beta chain vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Tubb4b0.000e+062.24tubulin, beta 4B class IVB [Source:MGI Symbol;Acc:... [more]
Tubb2b0.000e+062.00tubulin, beta 2B class IIB [Source:MGI Symbol;Acc:... [more]
Tubb50.000e+061.54tubulin, beta 5 class I [Source:MGI Symbol;Acc:MGI... [more]
Tubb4a0.000e+061.77tubulin, beta 4A class IVA [Source:MGI Symbol;Acc:... [more]
Tubb30.000e+059.82tubulin, beta 3 class III [Source:MGI Symbol;Acc:M... [more]
back to top
BLAST of Tubulin beta chain vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P41937|TBB4_CAEEL0.000e+062.41Tubulin beta-4 chain OS=Caenorhabditis elegans OX=... [more]
sp|Q9NFZ6|TBB2_ECHMU0.000e+063.17Tubulin beta-2 chain OS=Echinococcus multiloculari... [more]
sp|Q27U48|TBB1_GLOMM0.000e+062.70Tubulin beta-1 chain OS=Glossina morsitans morsita... [more]
sp|Q24560|TBB1_DROME0.000e+062.70Tubulin beta-1 chain OS=Drosophila melanogaster OX... [more]
sp|O17449|TBB1_MANSE0.000e+062.47Tubulin beta-1 chain OS=Manduca sexta OX=7130 PE=2... [more]
back to top
BLAST of Tubulin beta chain vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0X3PFM50.000e+062.47Tubulin beta chain OS=Schistocephalus solidus OX=7... [more]
C1LM620.000e+062.08Tubulin beta chain OS=Schistosoma japonicum OX=618... [more]
A0A0P5FPY00.000e+061.16Tubulin beta chain OS=Daphnia magna OX=35525 PE=3 ... [more]
A0A162PE160.000e+061.16Tubulin beta chain OS=Daphnia magna OX=35525 GN=AP... [more]
E9HEX50.000e+061.16Tubulin beta chain OS=Daphnia pulex OX=6669 GN=DAP... [more]
back to top
BLAST of Tubulin beta chain vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
zgc:554610.000e+062.00tubulin beta-4B chain [Source:NCBI gene;Acc:103024... [more]
TUBB2A0.000e+060.78tubulin beta-2A chain [Source:NCBI gene;Acc:103041... [more]
TUBB11.565e-16957.11tubulin beta 1 class VI [Source:HGNC Symbol;Acc:HG... [more]
ENSAMXT00000019550.22.194e-15262.06pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000019223.29.131e-9937.61tubulin alpha-1 chain [Source:NCBI gene;Acc:103031... [more]
back to top
BLAST of Tubulin beta chain vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tubb23.111e-16159.84tubulin, beta 2A class IIa [Source:ZFIN;Acc:ZDB-GE... [more]
TUBA3C1.754e-7738.16tubulin alpha 3c [Source:HGNC Symbol;Acc:HGNC:1240... [more]
tubg11.409e-7031.22tubulin, gamma 1 [Source:ZFIN;Acc:ZDB-GENE-040426-... [more]
ENSPMAT00000001368.11.670e-6129.48pep scaffold:Pmarinus_7.0:GL476337:866089:875956:-... [more]
ENSPMAT00000001139.18.024e-6032.49pep scaffold:Pmarinus_7.0:GL478075:30403:34872:1 g... [more]
back to top
BLAST of Tubulin beta chain vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 4
Match NameE-valueIdentityDescription
TUB22.214e-17656.01Beta-tubulin; associates with alpha-tubulin (Tub1p... [more]
TUB34.227e-10138.48Alpha-tubulin; associates with beta-tubulin (Tub2p... [more]
TUB18.450e-10137.79Alpha-tubulin; associates with beta-tubulin (Tub2p... [more]
TUB41.513e-3825.68Gamma-tubulin; involved in nucleating microtubules... [more]
back to top
BLAST of Tubulin beta chain vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO359580.000e+062.24Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO399450.000e+062.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO428900.000e+061.07Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO495188.522e-17856.82Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO439805.846e-17055.30Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of Tubulin beta chain vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tubb20.000e+062.47tubulin beta-2B chain [Source:NCBI gene;Acc:101162... [more]
tubb50.000e+061.54tubulin beta chain [Source:NCBI gene;Acc:101157969... [more]
ENSORLT00000045809.10.000e+062.47tubulin beta-6 chain [Source:NCBI gene;Acc:1011661... [more]
tuba1c5.298e-9838.62tubulin alpha-1B chain-like [Source:NCBI gene;Acc:... [more]
ENSORLT00000033163.12.528e-9738.01tubulin alpha-1A chain [Source:NCBI gene;Acc:10535... [more]
back to top
BLAST of Tubulin beta chain vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000057129.10.000e+0100.00SMESG000057129.1[more]
SMESG000047291.10.000e+063.17SMESG000047291.1[more]
SMESG000044888.10.000e+063.17SMESG000044888.1[more]
SMESG000050810.10.000e+063.64SMESG000050810.1[more]
SMESG000047284.10.000e+063.17SMESG000047284.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30000332 ID=SMED30000332|Name=Tubulin beta chain|organism=Schmidtea mediterranea sexual|type=transcript|length=1766bp
TGAATTATTAATTAAAAATATCTATTATATTAATTGTAATAAGTTCAAAA
TTATTAAAAAAAATGAGAGAAATTCTAAACATTCAAATGGGACAATGCGG
AAATAATATCGGAACAAAGTTTTGGGAATTAATTACTAATGAGCACGGCA
TTAATACGGTAACAGGAGTTTACGAGGGAGACAGCGATCTGCAATTGCAA
CGGATTGAGGTCTATTTTAATTCCGGAAATAATGGGAAATATGTCCCGAG
AAGTGTCATGGTGGACCTGGATAGCACGACCCTTGATTATATTTTGAATT
CAAATATTGGAGAATTGTTCCAGCCGGATTATTTCATCGGAGGAGCTTCC
AGTGCGTCAAATAATTTTGCAAAAGGCTTTTATACGGATGGAGCGGAAAT
AATTGATGAGATTATGGAACTAATACGAATTCAAGTGGAAGATTGTGATG
TGTTGCAAGGTTTTCAGATGTTTAACGGACTTGGTGGTGGAACGGGATCT
GGTTTGGGTTCTTTGATTCTAACGAAAATTAGAGAAGAATGGCCTGATAG
GATTATTAGTACAATTTCAATAGTGCCTTCTCCTAAAATTTCTGAAACAG
TTGTTGAGCCCTACAATTGTACTCTAGGAGCAAATTATCTTTCATATTTA
TCTGATGAAACATTTTGTATTGATAACGAAGCTCTTTACAGAATTTGTCG
AAACAATCTGAAATTACCCAATCCGACCTTTGAAGATCTAAATCATTTAA
TTTGCAACACAGTTTCTGGAATAACCACTTGCCTAAGATTTCCTGGACAA
CTCAATGCCGATTTGAGAAAATTAGCTGTAAATATGGTTCCTTTTCCCCG
ACTTCATTTCTTTATTCCCGCATTTGCTCCTTTAACAAGCCGATCAAATG
AAAATTATAAGGATTCCTTCAACGTCAATAGTCTTATTACAAACATGTTT
AAACCAGAAAATATGATGGCCGCCTGTAATCCGGCAAATGGAAAGTATTT
AACTATAGCAACGATTTTCCGGGGAAGAATGTCAACGAAAGAAGTTGAAG
AAGGAGTTTTGAGGGTTCGAGATAAAAATTTATCATGTTTTATAGATTGG
ATACCTTCCAATATCAAAACTGCGGTTTGTGATATACCTCCAAGAGGCTT
TAGAATTTCGGCAACTCTTATTGCTAATAATACAGCCATACAGGAATCAT
TTAGAAGAGTACATGAACAGTTTTTAGCTATGTACCGCCGTGCAGCGTTT
TTGCATTGGTACACGGGCGAAGGAATGGAAGCTTCAGAATTTATTGAAGC
TCACGATAATATGGTAGATTTATTAAATGAATATCAAATGTACGAAGAAA
AAGACAATGGTGATCATGGAGAAGACGATGGAAATCCCGAAGCCGACAAT
ATAAGCAAAGTAACTAAAGGTAAAAAAACAAGAGATAATTCCAAGAAATC
TTCCGCTAATAGCCAGTCAAAAACAGTGACGAAATCTGCAGTTTCGACTC
CTGAAGTAGTGAACAGCAAATCTAGCAAATCAAATGCCCAAAGTGATAGG
CAAAACGAATTGCGACCAAAAGATTCTTGGATTTCTAACGTTTCAACAGA
GCAATCTCTGACTCCTAAAAATATATTGAAGGTTAACTATTAGAATAATA
CTACTTATTGCTAGTCAATAATTGCTATTTTTCATTTGAAGAAAAATAAA
TCACTGACAATAGTATAATGAATATTATTATTTTTATAACTTTGCCAGTA
AAAAATTGAACTGCCA
back to top

protein sequence of SMED30000332-orf-1

>SMED30000332-orf-1 ID=SMED30000332-orf-1|Name=SMED30000332-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=527bp
MREILNIQMGQCGNNIGTKFWELITNEHGINTVTGVYEGDSDLQLQRIEV
YFNSGNNGKYVPRSVMVDLDSTTLDYILNSNIGELFQPDYFIGGASSASN
NFAKGFYTDGAEIIDEIMELIRIQVEDCDVLQGFQMFNGLGGGTGSGLGS
LILTKIREEWPDRIISTISIVPSPKISETVVEPYNCTLGANYLSYLSDET
FCIDNEALYRICRNNLKLPNPTFEDLNHLICNTVSGITTCLRFPGQLNAD
LRKLAVNMVPFPRLHFFIPAFAPLTSRSNENYKDSFNVNSLITNMFKPEN
MMAACNPANGKYLTIATIFRGRMSTKEVEEGVLRVRDKNLSCFIDWIPSN
IKTAVCDIPPRGFRISATLIANNTAIQESFRRVHEQFLAMYRRAAFLHWY
TGEGMEASEFIEAHDNMVDLLNEYQMYEEKDNGDHGEDDGNPEADNISKV
TKGKKTRDNSKKSSANSQSKTVTKSAVSTPEVVNSKSSKSNAQSDRQNEL
RPKDSWISNVSTEQSLTPKNILKVNY*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0003304testis primordium
Vocabulary: INTERPRO
TermDefinition
IPR036525Tubulin/FtsZ_GTPase_sf
IPR037103Tubulin/FtsZ_C_sf
IPR013838Beta-tubulin_BS
IPR000217Tubulin
IPR002453Beta_tubulin
IPR018316Tubulin/FtsZ_2-layer-sand-dom
IPR003008Tubulin_FtsZ_GTPase
IPR023123Tubulin_C
IPR017975Tubulin_CS
IPR008280Tub_FtsZ_C
Vocabulary: biological process
TermDefinition
GO:0007017microtubule-based process
GO:0007010cytoskeleton organization
Vocabulary: cellular component
TermDefinition
GO:0005874microtubule
GO:0005737cytoplasm
GO:0005856cytoskeleton
Vocabulary: molecular function
TermDefinition
GO:0005525GTP binding
GO:0005200structural constituent of cytoskeleton
GO:0003924GTPase activity
GO:0000166nucleotide binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002453Beta tubulinPRINTSPR01163BETATUBULINcoord: 109..127
score: 33.92
coord: 331..345
score: 34.59
coord: 349..372
score: 56.83
coord: 153..165
score: 64.09
coord: 266..282
score: 56.81
coord: 414..432
score: 43.1
coord: 246..259
score: 89.06
coord: 42..59
score: 54.41
coord: 89..100
score: 49.0
coord: 313..331
score: 64.54
coord: 213..225
score: 47.21
coord: 231..240
score: 50.4
coord: 379..390
score: 62.86
IPR000217TubulinPRINTSPR01161TUBULINcoord: 133..151
score: 66.98
coord: 152..173
score: 38.27
coord: 177..190
score: 62.95
coord: 10..30
score: 63.68
coord: 191..211
score: 49.67
coord: 52..71
score: 46.55
coord: 94..105
score: 58.07
coord: 107..131
score: 41.77
coord: 372..400
score: 58.74
IPR000217TubulinPANTHERPTHR11588TUBULINcoord: 2..442
IPR003008Tubulin/FtsZ, GTPase domainSMARTSM00864Tubulin_4coord: 48..245
e-value: 3.1E-49
score: 179.5
IPR003008Tubulin/FtsZ, GTPase domainPFAMPF00091Tubulincoord: 3..212
e-value: 1.6E-63
score: 214.5
IPR018316Tubulin/FtsZ, 2-layer sandwich domainSMARTSM00865Tubulin_C_4coord: 247..385
e-value: 9.2E-27
score: 104.9
IPR018316Tubulin/FtsZ, 2-layer sandwich domainPFAMPF03953Tubulin_Ccoord: 262..384
e-value: 3.9E-36
score: 124.0
IPR023123Tubulin, C-terminalGENE3DG3DSA:1.10.287.600coord: 376..446
e-value: 3.0E-23
score: 83.5
IPR036525Tubulin/FtsZ, GTPase domain superfamilyGENE3DG3DSA:3.40.50.1440coord: 1..264
e-value: 1.3E-102
score: 344.7
IPR036525Tubulin/FtsZ, GTPase domain superfamilySUPERFAMILYSSF52490Tubulin nucleotide-binding domain-likecoord: 1..244
IPR037103Tubulin/FtsZ, C-terminal domain superfamilyGENE3DG3DSA:3.30.1330.20coord: 265..375
e-value: 7.6E-43
score: 147.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 431..526
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 462..495
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 431..461
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 503..526
NoneNo IPR availablePANTHERPTHR11588:SF279TUBULIN BETA-4 CHAINcoord: 2..442
NoneNo IPR availableCDDcd02187beta_tubulincoord: 2..428
e-value: 0.0
score: 663.495
IPR017975Tubulin, conserved sitePROSITEPS00227TUBULINcoord: 141..147
IPR013838Beta tubulin, autoregulation binding sitePROSITEPS00228TUBULIN_B_AUTOREGcoord: 1..4
IPR008280Tubulin/FtsZ, C-terminalSUPERFAMILYSSF55307Tubulin C-terminal domain-likecoord: 245..430