SMED30000295
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30000295 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 22Note: Hover over icons to view figure legend
Homology
BLAST of SMED30000295 vs. TrEMBL
Match: A0A1S6KMJ8 (Uncharacterized protein (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 1040.8 bits (2690), Expect = 0.000e+0 Identity = 598/599 (99.83%), Postives = 598/599 (99.83%), Query Frame = 1 Query: 52 TWSTLSADNPYNGFETICIPQKACYFVSKFKTSWQTAKEMCLSKTHENILKFNDTDKNILNPLISTMETKRYWVVEPKESSNQKSHQITIILNRKRRSFVIEQPESETIITTVEQVKNWNWEQLIENTKDTDQSTIKRNIKKIFEAVKTSGNKNLDPNLLKKWKSIYEILSKSRIHQQFIRTQDQWTELTDEFGNGQLESFISTLDPKLQVEWKDKLSKVKWFVKNKEFNKLDLNSLNEWKNLIYKLRLKIHGKESYKKRLLVQWQSHVXXXXXXXXXXXXXFVSGNYKERVTKTVNELFVIIKRQEFEKINLELVTNVKFVMRLLKRLWRIHIKYGSRKKANMIRRLKLILSIDWRRIINELEGIDRNQWIERFNKLKNLIQLKRYNKIDVNWLAEFKPIIDKFRGKIKINYIHQXXXXXXXXXXXXXXXXWNWKNLVVDLXXXXXXXXXXXXXLMERIIKEEHFNEINVDLIKRFKVIFKILRKIKQSKYPKGNXXXXXXXXXXXXXXXXDSNWLNIINGLEGVDKVKWTTRFNKLIQMIRNNNIDKIDFKWLLKFKRILKLIHRKSHPRKQKLNLIKKFVNRLKKWNWPDLIKNID 1848 TWSTLSADNPYNGFETICIPQKACYFVSKFKTSWQTAKEMCLSKTHENILKFNDTDKNILNPLISTMETKRYWVVEPKESSNQKSHQITIILNRKRRSFVIEQPESETIITTVEQVKNWNWEQLIENTKDTDQSTIKRNIKKIFEAVKTSGNKNLDPNLLKKWKSIYEILSKSRIHQQFIRTQDQWTELTDEFGNGQLESFISTLDPKLQVEWKDKLSKVKWFVKNKEFNKLDLNSLNEWKNLIYKLRLKIHGKESYKKRLLVQWQSHVEWLKKWKWMELLKFVSGNYKERVTKTVNELFVIIKRQEFEKINLELVTNVKFVMRLLKRLWRIHIKYGSRKKANMIRRLKLILSIDWRRIINELEGIDRNQWIERFNKLKNLIQLKRYNKIDVNWLAEFKPIIDKFRGKIKINYIHQKRQIKKIRLYLRLLKRWNWKNLVVDLNEKEKENIAKEIELMERIIKEEHFNEINVDLIKRFKVIFKILRKIKQSKYPKGNIKVTKEILLKKIKLIIDSNWLNIINGLE VDKVKWTTRFNKLIQMIRNNNIDKIDFKWLLKFKRILKLIHRKSHPRKQKLNLIKKFVNRLKKWNWPDLIKNID Sbjct: 12 TWSTLSADNPYNGFETICIPQKACYFVSKFKTSWQTAKEMCLSKTHENILKFNDTDKNILNPLISTMETKRYWVVEPKESSNQKSHQITIILNRKRRSFVIEQPESETIITTVEQVKNWNWEQLIENTKDTDQSTIKRNIKKIFEAVKTSGNKNLDPNLLKKWKSIYEILSKSRIHQQFIRTQDQWTELTDEFGNGQLESFISTLDPKLQVEWKDKLSKVKWFVKNKEFNKLDLNSLNEWKNLIYKLRLKIHGKESYKKRLLVQWQSHVEWLKKWKWMELLKFVSGNYKERVTKTVNELFVIIKRQEFEKINLELVTNVKFVMRLLKRLWRIHIKYGSRKKANMIRRLKLILSIDWRRIINELEGIDRNQWIERFNKLKNLIQLKRYNKIDVNWLAEFKPIIDKFRGKIKINYIHQKRQIKKIRLYLRLLKRWNWKNLVVDLNEKEKENIAKEIELMERIIKEEHFNEINVDLIKRFKVIFKILRKIKQSKYPKGNIKVTKEILLKKIKLIIDSNWLNIINGLEEVDKVKWTTRFNKLIQMIRNNNIDKIDFKWLLKFKRILKLIHRKSHPRKQKLNLIKKFVNRLKKWNWPDLIKNID 610 HSP 2 Score: 79.337 bits (194), Expect = 1.065e-11 Identity = 77/240 (32.08%), Postives = 136/240 (56.67%), Query Frame = 1 Query: 1159 QWIERFNKLKNLIQLKRYNKIDVNWLAEFKPIIDKFRGKIKINYIHQXXXXXXXXXXXXXXXXWNWKNLVVDLXXXXXXXXXXXXXLMERIIKEEHFNEINVDLIKRFKVIFKILRKIKQSKYPKGNXXXXXXXXXXXXXXXXDSNWLNIINGLEGVDKVKWTTRFNKLIQMIRNNNIDKIDFKWLLKFKRILK---------LIHRKSHPRKQKLNLIKKFVNRLKKWNWPDLIKNIDE 1851 +W ++ +K+K ++ K +NK+D+N L E+K +I K R KI ++KR + + + ++ LK+W W L+ ++ KE + K + + IIK + F +IN++L+ K + ++L+++ + G+ K I K+ L ID W IIN LEG+D+ +W RFNKL +I+ +KID WL +FK I+ IH+K +K +L ++ LK+WNW +L+ +++E Sbjct: 223 EWKDKLSKVKWFVKNKEFNKLDLNSLNEWKNLIYKLRLKIHGKESYKKRLLVQWQSHVEWLKKWKWMELLKFVSGNYKERVTKTVNELFVIIKRQEFEKINLELVTNVKFVMRLLKRLWRIHIKYGSRKKANMIRRLKLILSID--WRRIINELEGIDRNQWIERFNKLKNLIQLKRYNKIDVNWLAEFKPIIDKFRGKIKINYIHQKRQIKKIRL-----YLRLLKRWNWKNLVVDLNE 455
BLAST of SMED30000295 vs. TrEMBL
Match: A0A1S6KMJ4 (Uncharacterized protein (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 382.874 bits (982), Expect = 9.656e-123 Identity = 227/487 (46.61%), Postives = 345/487 (70.84%), Query Frame = 1 Query: 58 STLSADNPYNGFETICIPQKACYFVSKFKTSWQTAKEMCLSKTHENILKFNDTDKNILNPLISTMETKRYWVVE-PKESSNQKSHQITIILNRKRRSFVIEQPESETIITTVEQVKNWNWEQLIENTKDTDQSTIKRNIKKIFEAVKTSGNKNLDPNLLKKWKSIYEILSKSRIHQQFIRTQDQWTELTDEFGNGQLESFISTLDPKLQVEWKDKLSKVKWFVKNKEFNKLDLNSLNEWKNLIYKLRLKIHGKESYKKRLLVQWQSHVXXXXXXXXXXXXXFVSGNYKERVTKTVNELFVIIKRQEFEKINLELVTNVKFVMRLLKRLWRIHIKYGSRKKANMIRRLKLILSIDWRRIINELEGIDRNQWIERFNKLKNLIQLKRYNKIDVNWLAEFKPIIDKFRGKIKINYIHQXXXXXXXXXXXXXXXXWNWKNLVVDLXXXXXXXXXXXXXLMERIIKEEHFNEINVDLIKRFKVIFKILRKIK 1515 + + ADN + FETICIP +ACY+VSKFKTSW+ A+EMC K ++ + FN +D ILNPL + +KRYWV+E K+ ++ +ITIILNRKRRSF+I +P +++TTV QVKNWNW+ L++ + + ++ +++ I+++F+ G++ L+ +K+WK Y++LS++R H++FIRT ++W LTDE G L+ +I LDPKL++ WK+KL+KV +KNK+FNK+D L +WK +IYKLRLKI+GKE+YK+++LV W+ HV+WL K+KW +LL++VS YK RVT +NEL I+K + +INLELV NV F +R++KRLW+IHI YG R+KA +I +KLIL+IDW I+ LE ++ +WI RF ++ +I+ K+YN++D+N L E KPII K R I + +R+I IR Y++LLK WNW L+ +++KE+ N+ IE M I+ ++ + EI+ + I++ + I + +RKIK Sbjct: 14 TNIQADNSNDPFETICIPDQACYYVSKFKTSWENAQEMCQEKIRQHSVPFNSSDNVILNPLADNLLSKRYWVLENKKDVVKEQPRKITIILNRKRRSFMIHRPGRHSVVTTVRQVKNWNWQNLVDKSNNVNRPQMRKTIERVFKQTNNGGSE-LNEETVKQWKKTYKVLSRTRTHRKFIRTYERWNILTDEVGKEHLKRYIDKLDPKLRILWKNKLNKVMLLIKNKQFNKIDPKLLLKWKQIIYKLRLKINGKENYKQKILVNWRKHVDWLLKFKWTDLLQYVSEKYKIRVTNALNELISIVKNNDLNRINLELVDNVNFAIRIIKRLWKIHIVYGGREKAEIIHEIKLILTIDWLTILERLENKEKEKWIPRFKQVIEVIKRKKYNELDLNLLLELKPIIKKIREVTNIKPLISERRINLIRRYVKLLKEWNWPELMKFVDQKERNNLIIGIEKMIGIVDQKRYKEIDNNFIQKLRFIVRFMRKIK 499 HSP 2 Score: 63.1586 bits (152), Expect = 1.352e-6 Identity = 74/248 (29.84%), Postives = 141/248 (56.85%), Query Frame = 1 Query: 1120 RRIINELEGIDRNQWIERFNKLKNLIQLKRYNKIDVNWLAEFKPIIDKFRGKIKINYIHQXXXXXXXXXXXXXXXXWNWKNLVVDLXXXXXXXXXXXXXLMERIIKEEHFNEINVDLIKRFKVIFKILRKIKQSKYPKGNXXXXXXXXXXXXXXXXDSNWLNIINGLEGVDKVKWTTRFNKLIQMIRNNNIDKIDFKWLLKFKRILKLIHRKSHPR----KQKLNLIKKFVNRLKKWNWPDLIKNIDE 1851 +R I++L+ R W + NK+ LI+ K++NKID L ++K II K R KI ++++ + R ++ L ++ W +L+ ++EK K + + + I+K N IN++L+ +I++++ + G + + I K+ L ID WL I+ LE +K KW RF ++I++I+ +++D LL+ K I+K I ++ + ++++NLI+++V LK+WNWP+L+K +D+ Sbjct: 210 KRYIDKLDPKLRILWKNKLNKVMLLIKNKQFNKIDPKLLLKWKQIIYKLRLKINGKENYKQKILVNWRKHVDWLLKFKWTDLLQYVSEKYKIRVTNALNELISIVKNNDLNRINLELVDNVNFAIRIIKRLWKIHIVYGGREKAEIIHEIKLILTID--WLTILERLENKEKEKWIPRFKQVIEVIKRKKYNELDLNLLLELKPIIKKIREVTNIKPLISERRINLIRRYVKLLKEWNWPELMKFVDQ 455
BLAST of SMED30000295 vs. TrEMBL
Match: A0A1S6KMJ1 (Uncharacterized protein (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 77.0258 bits (188), Expect = 6.599e-11 Identity = 66/177 (37.29%), Postives = 110/177 (62.15%), Query Frame = 1 Query: 1348 WNWKNLVVDLXXXXXXXXXXXXXLMERIIKEEHFNEINVDLIKRFKVIFKILRKIKQSKYPKGNXXXXXXXXXXXXXXXXDSNWLNIINGLEGVDKVKWTTRFNKLIQMIRNNNIDKIDFKWLLKFKRILKLIHRKSHPRKQKLNLIK---------KFVNRLKKWNWPDLIKNIDE 1851 WNW +L+ +++ KE+E KEI+++ +++KE F EI+ +L K+ +VI+K +RK+ + K PK I++ KE L+K+K II NW IIN + +D+VKW R N +I++++ N +DKID WL + ++I K+ K P ++ N+I F+ L + W +L+K IDE Sbjct: 27 WNWPDLIKNIDVKERERFQKEIDIVLKLVKENKFKEIDENLYKKIRVIYKKIRKVIKDKLPKVEIQIQKENSLEKLKPIITQNWAKIINETKNIDRVKWIDRINTIIKLVKLNEVDKIDLTWLKELEKIFKV---KEIPSTEE-NIIDIKTITDKSNNFLITLTELKWEELLKYIDE 199 HSP 2 Score: 61.2326 bits (147), Expect = 6.208e-6 Identity = 85/334 (25.45%), Postives = 170/334 (50.90%), Query Frame = 1 Query: 916 KERVTKTVNELFVIIKRQEFEKINLELVTNVKFVMRLLKR-----LWRIHIKYGSRKKANMIRRLKLILSIDWRRIINELEGIDRNQWIERFNKLKNLIQLKRYNKIDVNWLAEFKPIID-KFRGKIKINYIHQXXXXXXXXXXXXXXXXWNWKNLVVDLXXXXXXXXXXXXXLMERIIKEEHFNEINVDLIKRFKVIFKILRK-----IKQSKYPKGNXXXXXXXXXXXXXXXXDSNWLNIINGLEGVDKVKWTTRFNKLIQMIRNNNIDKID---FKW---LLKFKRILKLIHRKSHP-----RKQKLNLIKKFVNRLKKWNWPDLIKNIDE 1851 +ER K ++ + ++K +F++I+ L ++ + + +++ L ++ I+ +K N + +LK I++ +W +IINE + IDR +WI+R N + L++L +KID+ WL E + I K + N I K K +L L W+ L+ ++E + + +I+ +++ + + +I++DLI I L+K IK +K + N+K E K I ++++ NW ++ ++ ++++ + I ++N +KID W ++ R +K +H K+ RK + K+VN L + NW +L+K I+E Sbjct: 41 RERFQKEIDIVLKLVKENKFKEIDENLYKKIRVIYKKIRKVIKDKLPKVEIQI---QKENSLEKLKPIITQNWAKIINETKNIDRVKWIDRINTIIKLVKLNEVDKIDLTWLKELEKIFKVKEIPSTEENIIDIKTITDKSNNFLITLTELKWEELLKYIDESSRPEVLNKIKTFVTLVENKQYEKIDIDLITWITEIITTLKKAKEVQIKNTKTEEVNLKEISETSNKFIDILVEINWEELVKYIDQSNRLEVINKIKTFITFVKNKQYEKIDTNLINWINHIITIFRQVKEVHIKNEKITNDNRKLITDTSNKYVNFLIELNWEELVKQIEE 371
BLAST of SMED30000295 vs. Planmine SMEST
Match: SMESG000008835.1 (SMESG000008835.1) HSP 1 Score: 1041.18 bits (2691), Expect = 0.000e+0 Identity = 593/600 (98.83%), Postives = 597/600 (99.50%), Query Frame = 1 Query: 52 TWSTLSADNPYNGFETICIPQKACYFVSKFKTSWQTAKEMCLSKTHENILKFNDTDKNILNPLISTMETKRYWVVEPKESSNQKSHQITIILNRKRRSFVIEQPESETIITTVEQVKNWNWEQLIENTKDTDQSTIKRNIKKIFEAVKTSGNKNLDPNLLKKWKSIYEILSKSRIHQQFIRTQDQWTELTDEFGNGQLESFISTLDPKLQVEWKDKLSKVKWFVKNKEFNKLDLNSLNEWKNLIYKLRLKIHGKESYKKRLLVQWQSHVXXXXXXXXXXXXXFVSGNYKERVTKTVNELFVIIKRQEFEKINLELVTNVKFVMRLLKRLWRIHIKYGSRKKANMIRRLKLILSIDWRRIINELEGIDRNQWIERFNKLKNLIQLKRYNKIDVNWLAEFKPIIDKFRGKIKINYIHQXXXXXXXXXXXXXXXXWNWKNLVVDLXXXXXXXXXXXXXLMERIIKEEHFNEINVDLIKRFKVIFKILRKIKQSKYPKGNXXXXXXXXXXXXXXXXDSNWLNIINGLEGVDKVKWTTRFNKLIQMIRNNNIDKIDFKWLLKFKRILKLIHRKSHPRKQKLNLIKKFVNRLKKWNWPDLIKNIDE 1851 TWSTLSADNPYNGFETICIPQKACYFVSKFKTSWQTAKEMCLSKTHENILKFNDTDKNILNPLISTMETKRYWVVEPKESSNQKSHQITIILNRKRRSFVIEQPESETIITTVEQVKNWNWEQLIENTKDTDQSTIKRNIKKIFEAVKTSGNKNLDPNLLKKWKSIYEILSKSRIHQQFIRTQDQWTELTDEFGNGQLESFISTLDPKLQVEWKDKLSKVKWFVKNKEFNKLDLNSLNEWKNLIYKLRLKIHGKESYKKRLLVQWQSHVEWLKKWKWMELLKFVSGNYKERVTKTVNELFVIIKRQEFEKINLELV NVKFVMRLLKRLWRIHIKYGSRKKANMIRRLKLILSIDWRRIINELEGIDRNQWIERFNKLKNLIQLKRYN+IDVNWLAEFKPIIDKFRGKIKINYIHQKRQ KKIRLYLRLLKRWNWKNLVVDLNEKEKENIAKEIELM+RIIKEEHFNEINVDLIKRFKVIFKILRKIKQSKYPKGN+KVTKEILLKKIKLIIDSNWLNIINGLEGVDKVKWTTRFNK+IQMIRNNNIDKIDFKWLLKFKRILKLIHRKSHPRKQKLNLIKKFVNRL KWNWPDLIKNIDE Sbjct: 12 TWSTLSADNPYNGFETICIPQKACYFVSKFKTSWQTAKEMCLSKTHENILKFNDTDKNILNPLISTMETKRYWVVEPKESSNQKSHQITIILNRKRRSFVIEQPESETIITTVEQVKNWNWEQLIENTKDTDQSTIKRNIKKIFEAVKTSGNKNLDPNLLKKWKSIYEILSKSRIHQQFIRTQDQWTELTDEFGNGQLESFISTLDPKLQVEWKDKLSKVKWFVKNKEFNKLDLNSLNEWKNLIYKLRLKIHGKESYKKRLLVQWQSHVEWLKKWKWMELLKFVSGNYKERVTKTVNELFVIIKRQEFEKINLELVKNVKFVMRLLKRLWRIHIKYGSRKKANMIRRLKLILSIDWRRIINELEGIDRNQWIERFNKLKNLIQLKRYNEIDVNWLAEFKPIIDKFRGKIKINYIHQKRQKKKIRLYLRLLKRWNWKNLVVDLNEKEKENIAKEIELMKRIIKEEHFNEINVDLIKRFKVIFKILRKIKQSKYPKGNVKVTKEILLKKIKLIIDSNWLNIINGLEGVDKVKWTTRFNKIIQMIRNNNIDKIDFKWLLKFKRILKLIHRKSHPRKQKLNLIKKFVNRLIKWNWPDLIKNIDE 611 HSP 2 Score: 194.897 bits (494), Expect = 3.405e-52 Identity = 179/495 (36.16%), Postives = 301/495 (60.81%), Query Frame = 1 Query: 406 WNWEQLIENTKDTDQSTIKRNIKKIFEAVKTSGNKNLDPNLLKKWKSIYEILSKSRIHQQ-FIRTQDQWTELTDEFG---NGQLESFISTLDPKLQVEWKDKLSKVKWFVKNKEFNKLDLNSLNEWKNLIYKLRLKIHGKESYKKRLLVQWQSHVXXXXXXXXXXXXXFVSGNYKERVTKTVNELFVIIKRQEFEKINLELVTNVKFVMRLLKRLWR-----IHIKYGSRKKANMIRRLKLILSIDWRRIINELEGIDRNQWIERFNKLKNLIQLKRYNKIDVNWLAEFKPIIDKFRGKIKINYIHQXXXXXXXXXXXXXXXXWNWKNLVVDLXXXXXXXXXXXXXLMERIIKEEHFNEINVDLIKRFKVIFKILRKIKQSKYPKGNXXXXXXXXXXXXXXXXDSNWLNIINGLEGVDKVKWTTRFNKLIQMIRNNNIDKIDFKWLLKFKRILKLIHRKSHPRKQKLNLI----KKFVNRLKKWNWPDLIKNIDE 1851 WNW LI+N + D+ +K+ I+ + + VK + K +D NL K+ + I++ + K + ++ I + Q L ++ + + I+ +V+W ++++K+ +K + +K+DL L ++K +I ++ K K + + ++ L KW W +L+K + +E V K + + ++K +F++I+ L ++ + + ++++ I IK +K ++ +LKLI+ +W+++INE +GID+ +W R NKL LI+L + +KID+ WL +FK II+ + + I K K ++ +++ L +WNW +L+ +++EK++EN+ KEIEL+ +++KE F EI+ +L KR +VIFK +RKIK K P +IKV KE LL K+KLIID+NW +IN +G+DKVKWT R NKLIQ+I+ N DKID KWL KF R++K++ +K+ R + +I KKFVNRL KWNW DLIKNIDE Sbjct: 1080 WNWSDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFKRIINIVKKNAIKKPITVKIITKNVKRFIKRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKKPIIDIKV---QKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFKRIINIVKKNVIKKPITVKIITKNVKRFIKRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKKPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKFVNRLIKWNWSDLIKNIDE 1571 HSP 3 Score: 194.512 bits (493), Expect = 3.986e-52 Identity = 179/495 (36.16%), Postives = 301/495 (60.81%), Query Frame = 1 Query: 406 WNWEQLIENTKDTDQSTIKRNIKKIFEAVKTSGNKNLDPNLLKKWKSIYEILSKSRIHQQ-FIRTQDQWTELTDEFG---NGQLESFISTLDPKLQVEWKDKLSKVKWFVKNKEFNKLDLNSLNEWKNLIYKLRLKIHGKESYKKRLLVQWQSHVXXXXXXXXXXXXXFVSGNYKERVTKTVNELFVIIKRQEFEKINLELVTNVKFVMRLLKRLWR-----IHIKYGSRKKANMIRRLKLILSIDWRRIINELEGIDRNQWIERFNKLKNLIQLKRYNKIDVNWLAEFKPIIDKFRGKIKINYIHQXXXXXXXXXXXXXXXXWNWKNLVVDLXXXXXXXXXXXXXLMERIIKEEHFNEINVDLIKRFKVIFKILRKIKQSKYPKGNXXXXXXXXXXXXXXXXDSNWLNIINGLEGVDKVKWTTRFNKLIQMIRNNNIDKIDFKWLLKFKRILKLIHRKSHPRKQKLNLI----KKFVNRLKKWNWPDLIKNIDE 1851 WNW LI+N + D+ +K+ I+ + + VK + K +D NL K+ + I++ + K + ++ I + Q L ++ + + I+ +V+W ++++K+ +K + +K+DL L ++K +I ++ K K + + ++ L KW W +L+K + +E V K + + ++K +F++I+ L ++ + + ++++ I IK +K ++ +LKLI+ +W+++INE +GID+ +W R NKL LI+L + +KID+ WL +FK II+ + + I K K ++ +++ L +WNW +L+ +++EK++EN+ KEIEL+ +++KE F EI+ +L KR +VIFK +RKIK K P +IKV KE LL K+KLIID+NW +IN +G+DKVKWT R NKLIQ+I+ N DKID KWL KF R++K++ +K+ R + +I KKFVNRL KWNW DLIKNIDE Sbjct: 600 WNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFKRIINIVKKNAIKKPITVKIITKNVKRFIKRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKKPIIDIKV---QKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFKRIINIVKKNVIKKPITVKIITKNVKRFIKRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKKPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKFVNRLIKWNWSDLIKNIDE 1091 HSP 4 Score: 192.971 bits (489), Expect = 1.586e-51 Identity = 181/519 (34.87%), Postives = 310/519 (59.73%), Query Frame = 1 Query: 349 VIEQPESETIITT-----VEQVKNWNWEQLIENTKDTDQSTIKRNIKKIFEAVKTSGNKNLDPNLLKKWKSIYEILSKSRIHQQ-FIRTQDQWTELTDEFG---NGQLESFISTLDPKLQVEWKDKLSKVKWFVKNKEFNKLDLNSLNEWKNLIYKLRLKIHGKESYKKRLLVQWQSHVXXXXXXXXXXXXXFVSGNYKERVTKTVNELFVIIKRQEFEKINLELVTNVKFVMRLLKRLWR-----IHIKYGSRKKANMIRRLKLILSIDWRRIINELEGIDRNQWIERFNKLKNLIQLKRYNKIDVNWLAEFKPIIDKFRGKIKINYIHQXXXXXXXXXXXXXXXXWNWKNLVVDLXXXXXXXXXXXXXLMERIIKEEHFNEINVDLIKRFKVIFKILRKIKQSKYPKGNXXXXXXXXXXXXXXXXDSNWLNIINGLEGVDKVKWTTRFNKLIQMIRNNNIDKIDFKWLLKFKRILKLIHRKSHPRKQKLNLI----KKFVNRLKKWNWPDLIKNIDE 1851 I++P + IIT ++++ WNW LI+N + D+ +K+ I+ + + VK + K +D NL K+ + I++ + K + ++ I + Q L ++ + + I+ +V+W ++++K+ +K + +K+DL L ++K +I ++ + K K + + ++ L KW W +L+K + +E V K + + ++K +F++I+ L ++ + + ++++ I IK +K ++ +LKLI+ +W+++INE +GID+ +W R NKL LI+L + +KID+ WL +F +I R K I K K ++ ++ L +WNW +L+ +++EK++EN+ KEIEL+ +++KE F EI+ +L KR +VIFK +RKIK K P +IKV KE LL K+KLIID+NW +IN +G+DKVKWT R NKLIQ+I+ N DKID KWL KFKRI+ ++ + + + + +I K+F+ RL KWNWPDLIKNIDE Sbjct: 1216 AIKKPITVKIITKNVKRFIKRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKKPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFKRIINIVKKNVIKKPITVKIITKNVKRFIKRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKKPIIDIKV---QKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKFVNRLIKWNWSDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFKRIINIVKKNAIKKPITVKIITKNVKRFIKRLIKWNWPDLIKNIDE 1731 HSP 5 Score: 142.124 bits (357), Expect = 9.712e-35 Identity = 156/523 (29.83%), Postives = 292/523 (55.83%), Query Frame = 1 Query: 349 VIEQPESETIITT-----VEQVKNWNWEQLIENTKDTDQSTIKRNIKKIFEAVKTSGNKNLDPNLLKKWKSIYEILSKSRIHQQ-FIRTQDQWTELTDEFG---NGQLESFISTLDPKLQVEWKDKLSKVKWFVKNKEFNKLDLNSLNEWKNLIYKLRLKIHGKESYKKRLLVQWQSHVXXXXXXXXXXXXXFVSGNYKERVTKTVNELFVIIKRQEFEKINLELVTNVKFVMRLLKRLWR-----IHIKYGSRKKANMIRRLKLILSIDWRRIINELEGIDRNQWIERFNKLKNLIQLKRYNKIDVNWLAEFKPIIDKFRGKIKINYIHQXXXXXXXXXXXXXXXXWNWKNLVVDLXXXXXXXXXXXXXLMERIIKEEHFNEINVDLIKRFKVIFKILRKIKQSKYPKGNXXXXXXXXXXXXXXXXDSNWLNIINGLEGVDKVKWTTRFNKLIQMIRNNNIDKIDFKWLLKFKRILKLIHRKSHPRKQ----KLNLIKK----FVNRLKKWNWPDLIKNIDE 1851 VI++P + IIT ++++ WNW LI+N + D+ +K+ I+ + + VK + K +D NL K+ + I++ + K + ++ I + Q L ++ + + I+ +V+W ++++K+ +K + +K+DL L ++ LI +R K + K + + V L KW W +L+K + +E V K + + ++K +F++I+ L ++ + + ++++ I IK +K ++ +LKLI+ +W+++INE +GID+ +W R NKL LI+L + +KID+ WL +FK II+ + I K K ++ +++ L +WNW +L+ +++EK++EN+ KEIEL+ +++KE F EI+ +L KR +VIFK +RK+K K P ++ KE +L K+K I++ NW II +DK KWTTR + ++I + DKID+ WLL+ +L++ + P ++ L ++ + + L + NW +L+K I E Sbjct: 1376 VIKKPITVKIITKNVKRFIKRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKKPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKFVNRLIKWNWSDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIKV---QKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFKRIINIVKKNAIKKPITVKIITKNVKRFIKRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKKVRKVKIDKIPTEKKQLLKEKILTKLKPILNENWTKIIEKSTNIDKTKWTTRIKTITELINSKQTDKIDYSWLLE---LLEIFNFPDEPDREIPTIDLAVVTEKSISLITDLNQLNWEELVKYIGE 1892 HSP 6 Score: 78.9518 bits (193), Expect = 8.810e-15 Identity = 123/522 (23.56%), Postives = 249/522 (47.70%), Query Frame = 1 Query: 412 WEQLIENTKDTDQSTIKRNIKKIFEAVKTSGNKNLDPNLLKKWKSIYEILSKSR---IHQQFIRTQD--QWTELTDEFGNG----QLESFISTLDPKLQVEWKDKLSKVKWFVKNKEFNKLDLNSLNEWKNLIYKLRLKIHGKESYK----------KRLLVQWQSHVXXXXXXXXXXXXXFVSGNYKERVTKTVNELFVIIKRQEFEKINLELVTNVKFVMRLLKRLWRIHIKYGSRKKANM-------IRRLKLILSIDWRRIINELEGIDRNQWIERFNKLKNLIQLKRYNKIDVNWLAEFKPIIDKFRGKIKINYIHQXXXXXXXXXXXXXXXXW-------NWKNLVVDLXXXXXXXXXXXXXLMERIIKEEHFNEINVDLIKRFKVIFKILRKIK--QSKYPKGNXXXXXXXXXXXXXXXXDSNWLNIINGLEGVDKVKWTTRFNKLIQMIRNNNIDKIDFKWLLKFKRILKLIHRKSHPRKQKLNL-------IKKFVNRLKKWNWPDLIKNIDE 1851 WE+L + + ++ T+ IK V+ +N+D +LL I + L K+R + + +T + TE + ++ N + E +D K + DK+ FV+NKEF +D++ LN +I LR + +ES+ K + ++ L + KW EL K++ ++ V + ++ +EFE I+++L+ + +++ L+ H+ K +N+ I+ + + + W + ++ +R I++ ++ K + ID++ L II R +IN ++K +IK ++ ++ W+ LV ++ + + +I+ ++ + F++I+V+L I ILRKI+ QSK + + + I+L + SNW ++ + G+D WT+R ++L+ ++++ N KIDF+W+ + ++I+ LI ++S K+++ L +K FV++LK+WNW L+ IDE Sbjct: 1967 WEELTKYIDEKNRQTVIDKIKTFIRFVENKEFENIDIDLLNWILEIIKTLRKARESHVTNEKTKTSNLKTITETSIKYINSLHELKWEELTKYIDEKNRQTVIDKIKTFIRFVENKEFENIDIDLLNWILEIIKTLR---NARESHVTNEKTKTSNLKTITETSIKYINSLHELKWEELTKYIDEKNRQTVIDKIKTFIRFVENKEFENIDIDLLNWILEIIKTLRNARESHVTNEKTKTSNLKTITETSIKYINSLHELKWEELTKYIDEKNRQTVIDKIKTFIRFVENKEFENIDIDLLNWILEIIKTLRKAREINETNEKVKIKNLKTLTETSLKYFNYLIELKWEELVQHIDSSSRVEVINKIKTFISFVQNKSFDKIDVNLANWIAEIINILRKIRIGQSKVTEN----KTLKITENIQLTLPSNWQELLQRINGIDYQLWTSRIDQLLTIMKSKNHQKIDFEWIGELEKIVNLISQESIKMKKEVFLNETSIEKLKGFVSQLKEWNWNSLLIYIDE 2481 HSP 7 Score: 60.077 bits (144), Expect = 5.943e-9 Identity = 48/207 (23.19%), Postives = 111/207 (53.62%), Query Frame = 1 Query: 901 VSGNYKERVTKTVNELFVIIKRQEFEKINLELVTNVKFVMRLLKRLWRIHIKYGSRKKANMIRRLKLILSIDWRRIINELEGIDRNQWIERFNKLKNLIQLKRYNKIDVNWLAEFKPIIDKFRGKI---KINYIHQXXXXXXXXXXXXXXXXWNWKNLVVDLXXXXXXXXXXXXXLMERIIKEEHFNEINVDLIKRFKVIFKILRKI 1512 + G+ K + + + +++ +E++KI+ E + + ++ +K+ + + + K +I ++++ L +W+ ++ +EGID N W R L +L+Q K Y KID W+ E + I+ ++ K ++ + KI+ + LK+WNW++L+ + E ++ + +++ M I++ E F++I+ ++ F I ++ KI Sbjct: 3288 IEGDNKPEIKEKIKRFITLVQNREYDKIDTEFLNWLFEIIENIKKTKQKLTETDNTKNVKVIEKIQINLPSNWKELLQNVEGIDVNLWTARIENLLSLVQSKEYQKIDFEWIDELQKIVQLISTQVTESKEKFVLPESTKTKIKENINSLKQWNWESLLKYIEETNRKQVETDVKKMIVIVENEEFDKIDSQFVQWFNQIVELFNKI 3494 HSP 8 Score: 59.6918 bits (143), Expect = 6.309e-9 Identity = 33/94 (35.11%), Postives = 56/94 (59.57%), Query Frame = 1 Query: 1591 SNWLNIINGLEGVDKVKWTTRFNKLIQMIRNNNIDKIDFKWLLKFKRILKLIHRKSHPRKQKLNL-------IKKFVNRLKKWNWPDLIKNIDE 1851 SNW ++ +EG+D WT R L+ ++++ KIDF+W+ + ++I++LI + K+K L IK+ +N LK+WNW L+K I+E Sbjct: 3358 SNWKELLQNVEGIDVNLWTARIENLLSLVQSKEYQKIDFEWIDELQKIVQLISTQVTESKEKFVLPESTKTKIKENINSLKQWNWESLLKYIEE 3451 HSP 9 Score: 56.225 bits (134), Expect = 8.915e-8 Identity = 90/403 (22.33%), Postives = 192/403 (47.64%), Query Frame = 1 Query: 367 SETIITTVEQVKNWNWEQLIENTKDTDQSTIKRNIKKIFEAVKTSGNKNLDPNLLKKWKSIYEILSKSR---IHQQFIRTQD--QWTELTDEFGNG----QLESFISTLDPKLQVEWKDKLSKVKWFVKNKEFNKLDLNSLNEWKNLIYKLRLKIHGKESYKKRLLVQWQS-------HVXXXXXXXXXXXXXFVSGNYKERVTKTVNELFVIIKRQEFEKINLELVTNVKFVMRLLKRLWRIHIKYGSRKKANMIRRLKLILSIDWRRIINELEGIDRNQWIERFNKLKNLIQLKRYNKIDVNWLAEFKPIIDKFRG---KIKINYIHQXXXXXXXXXXXXXXXXWNWKNLVVDLXXXXXXXXXXXXXLMERIIKEEHFNEINVDLIKRFKVIFKILRKIKQ 1518 +ET I + + WE+L + + ++ T+ IK V+ +N+D +LL I + L +R + + +T + TE + ++ N + E +D K + DK+ FV+NKEF +D++ LN +I LR E+ +K + ++ + +L + KW EL++ + + + V + ++ + F+KI++ L + ++ +L+++ K K + ++L L +W+ ++ + GID W R ++L +++ K + KID W+ E + I++ K+K + I+K++ ++ LK WNW +L++ ++E ++ I EI+ + +++ +F ++ + + F IF+ KIK+ Sbjct: 2124 TETSIKYINSLHELKWEELTKYIDEKNRQTVIDKIKTFIRFVENKEFENIDIDLLNWILEIIKTLRNARESHVTNEKTKTSNLKTITETSIKYINSLHELKWEELTKYIDEKNRQTVIDKIKTFIRFVENKEFENIDIDLLNWILEIIKTLRKAREINETNEKVKIKNLKTLTETSLKYFNYLIELKWEELVQHIDSSSRVEVINKIKTFISFVQNKSFDKIDVNLANWIAEIINILRKIRIGQSKVTENKTLKITENIQLTLPSNWQELLQRINGIDYQLWTSRIDQLLTIMKSKNHQKIDFEWIGELEKIVNLISQESIKMKKEVFLNETSIEKLKGFVSQLKEWNWNSLLIYIDENNRQQIQNEIQGLISVVESGNFEKLEIQTVNWFYQIFETFSKIKE 2526 HSP 10 Score: 54.299 bits (129), Expect = 3.600e-7 Identity = 82/337 (24.33%), Postives = 158/337 (46.88%), Query Frame = 1 Query: 358 QPESETIITTVEQVKNWNWEQLIENTKDTDQSTIKRNIKKIFEAVKTSGNKNLDPNLLKKWKSIYEILSKSRIHQQFIRTQDQWTELTDEFGNGQLESFISTLDPKLQVEWKDKLSKVKWFVKN-------------KEFNKLDLNSLNEWKNLIYKLRLKIHGKESYKKRLLVQWQSHVXXXXXXXXXXXXXFVSGNYKERVTKTVNELFVIIKRQEFEKINLEL---VTNVKFVMRLLKRLWRIHIKYGSRKKANMIRRLKLIL-----------SIDWRRIINELEGIDRNQWIERFNKLKNLIQLKRYNKID---VNWLAEFKPIIDKFRGKI 1278 Q +TI T + +K WNWE ++ + + I+ NI KI ++ + LDP+ + W FI+ +++ ++ NG + + K VE ++K+ K + F K K NK+ +N+L WK + K KI KE +KR++ + V WL+KW W L+K+V N+K + + +IK ++++ I+ +L ++ +K +++++++ ++ N+ LK+++ + W II+E+EG ++ + E+ + L+Q + Y+KID +NWL E I K + K+ Sbjct: 3027 QVSIQTIRTNINSLKLWNWENFLQYIDEDKREPIQTNINKIINVIENGESDKLDPDTI-DW---------------FIQIFEEFKKINYAIENGYVYDPNTRTWTKNTVEVENKIRKKRCFSKGHRKIRKFRPHNNLKFHNKIHINNLFHWKKIHQKHHRKIVSKE--RKRIVHKVIKRVNWLEKWNWKNLMKYVVDNHKRDTLVKIKYIISLIKNKDYDNIDEDLLKWISTIKTKLKIVRKI--------KKETKNIDENLKIVMKKSHKFVDILNQLRWIDIIDEIEGDNKPEIKEKIKRFITLVQNREYDKIDTEFLNWLFEIIENIKKTKQKL 3337 HSP 11 Score: 51.2174 bits (121), Expect = 2.844e-6 Identity = 33/94 (35.11%), Postives = 52/94 (55.32%), Query Frame = 1 Query: 1591 SNWLNIINGLEGVDKVKWTTRFNKLIQMIRNNNIDKIDFKWLLKFKRILKLIHRKS-----HPRKQKLNL--IKKFVNRLKKWNWPDLIKNIDE 1851 SNW +I L ++ W R L+ MI N DKIDF W+ + ++++ LI +S P+ ++ + +K+ VN LK+WNW +K IDE Sbjct: 2822 SNWKEVIVKLPNINVDLWIKRIQLLLPMIELNEFDKIDFGWIDELQKLVDLITEESIKTVEKPKVNEVTIKTVKENVNSLKQWNWETYLKYIDE 2915
BLAST of SMED30000295 vs. Planmine SMEST
Match: SMESG000008843.1 (SMESG000008843.1) HSP 1 Score: 387.497 bits (994), Expect = 5.596e-118 Identity = 229/487 (47.02%), Postives = 348/487 (71.46%), Query Frame = 1 Query: 58 STLSADNPYNGFETICIPQKACYFVSKFKTSWQTAKEMCLSKTHENILKFNDTDKNILNPLISTMETKRYWVVE-PKESSNQKSHQITIILNRKRRSFVIEQPESETIITTVEQVKNWNWEQLIENTKDTDQSTIKRNIKKIFEAVKTSGNKNLDPNLLKKWKSIYEILSKSRIHQQFIRTQDQWTELTDEFGNGQLESFISTLDPKLQVEWKDKLSKVKWFVKNKEFNKLDLNSLNEWKNLIYKLRLKIHGKESYKKRLLVQWQSHVXXXXXXXXXXXXXFVSGNYKERVTKTVNELFVIIKRQEFEKINLELVTNVKFVMRLLKRLWRIHIKYGSRKKANMIRRLKLILSIDWRRIINELEGIDRNQWIERFNKLKNLIQLKRYNKIDVNWLAEFKPIIDKFRGKIKINYIHQXXXXXXXXXXXXXXXXWNWKNLVVDLXXXXXXXXXXXXXLMERIIKEEHFNEINVDLIKRFKVIFKILRKIK 1515 + + ADN + FETICIP +ACY+VSKFKTSW+ A+EMC K ++ + FN +D ILNPL + +KRYWV+E K+ N++ +ITIILNRKRRSF+I +P +++TTV QVKNWNW+ L++ + + ++ +++ I+++F+ G++ L+ +K+WK Y++LS++R H++FIRT ++W LTDE G L+ +I LDPKL++ WK+KL+KV +KNK+FNK+D L +WK +IYKLRLKI+GKE+YK+++LV W+ HV+WL K+KW +LL++VS YK RVT +NEL I+K + +INLELV NV F +R++KRLW+IHI YG R+KA +I +KLIL+IDW +I+ LE ++ +WI RF ++ +I+ K+YN++D+N L E KPII K R KI + +R+I IR Y++LLK WNW L+ +++KE+ N+ IE M I+ ++ + EI+ + I++ + I + +RKIK Sbjct: 14 TNIQADNSNDPFETICIPDQACYYVSKFKTSWENAQEMCQEKIRQHSVPFNSSDNVILNPLADNLLSKRYWVLENKKDVVNEQPRKITIILNRKRRSFMIHRPGRHSVVTTVRQVKNWNWQNLVDKSNNVNRPQMRKTIERVFKQTNNGGSE-LNEETVKQWKKTYKVLSRTRTHRKFIRTYERWNILTDEVGKEHLKRYIDKLDPKLRILWKNKLNKVMLLIKNKQFNKIDPKLLLKWKQIIYKLRLKINGKENYKQKILVNWRKHVDWLLKFKWTDLLQYVSEKYKIRVTNALNELISIVKNNDLNRINLELVDNVNFAIRIIKRLWKIHIVYGGREKAEIIHEIKLILTIDWLKILERLENKEKEKWIPRFKQVIEVIKRKKYNELDLNLLLELKPIIKKIREVTKIKPLISERRINLIRRYVKLLKEWNWPELMKFVDQKERNNLIIGIEKMIGIVDQKRYKEIDNNFIQKLRFIVRFMRKIK 499 HSP 2 Score: 200.29 bits (508), Expect = 4.635e-54 Identity = 182/495 (36.77%), Postives = 298/495 (60.20%), Query Frame = 1 Query: 406 WNWEQLIENTKDTDQSTIKRNIKKIFEAVKTSGNKNLDPNLLKKWKSIYEILSKSRIHQQ-FIRTQDQWTELTDEFG---NGQLESFISTLDPKLQVEWKDKLSKVKWFVKNKEFNKLDLNSLNEWKNLIYKLRLKIHGKESYKKRLLVQWQSHVXXXXXXXXXXXXXFVSGNYKERVTKTVNELFVIIKRQEFEKINLELVTNVKFVMRLLKRLWR-----IHIKYGSRKKANMIRRLKLILSIDWRRIINELEGIDRNQWIERFNKLKNLIQLKRYNKIDVNWLAEFKPIIDKFRGKIKINYIHQXXXXXXXXXXXXXXXXWNWKNLVVDLXXXXXXXXXXXXXLMERIIKEEHFNEINVDLIKRFKVIFKILRKIKQSKYPKGNXXXXXXXXXXXXXXXXDSNWLNIINGLEGVDKVKWTTRFNKLIQMIRNNNIDKIDFKWLLKFKRILKLIHRKSHPRKQKLNLI----KKFVNRLKKWNWPDLIKNIDE 1851 WNW LI+N + D+ +K+ I+ + + VK + K +D NL K+ + I++ + K + ++ I + Q L ++ + + I+ +V+W ++++K+ +K + +K+DL L ++ LI +R K + K + + V L KW W +L+K + +E V K + + ++K +F++I+ L ++ + + ++++ I IK +K ++ +LKLI+ +W+++INE +GID+ +W R NKL LI+L + +KID+ WL +F +I R K I K K ++ ++ L +WNW +L+ +++EK++EN+ KEIEL+ +++KE F EI+ +L KR +VIFK +RKIK K P +IKV KE LL K+KLIID+NW +IN +G+DKVKWT R NKLIQ+I+ N DKID KWL KF R++K++ +K+ R + +I KKFVNRL KWNWPDLIKNIDE Sbjct: 847 WNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKFVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKKPIIDIKV---QKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKFVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKFVNRLIKWNWPDLIKNIDE 1338 HSP 3 Score: 200.29 bits (508), Expect = 5.739e-54 Identity = 180/491 (36.66%), Postives = 292/491 (59.47%), Query Frame = 1 Query: 406 WNWEQLIENTKDTDQSTIKRNIKKIFEAVKTSGNKNLDPNLLKKWKSIYEILSKSRIHQQFIRTQDQWTELTDEFGNGQLESFISTLDPKLQVEWKDKLSKVKWFVKNKEFNKLDLNSLNEWKNLIYKLRLKIHGKESYKKRLLVQWQSHVXXXXXXXXXXXXXFVSGNYKERVTKTVNELFVIIKRQEFEKINLELVTNVKFVMRLLKRLWR-----IHIKYGSRKKANMIRRLKLILSIDWRRIINELEGIDRNQWIERFNKLKNLIQLKRYNKIDVNWLAEFKPIIDKFRGKIKINYIHQXXXXXXXXXXXXXXXXWNWKNLVVDLXXXXXXXXXXXXXLMERIIKEEHFNEINVDLIKRFKVIFKILRKIKQSKYPKGNXXXXXXXXXXXXXXXXDSNWLNIINGLEGVDKVKWTTRFNKLIQMIRNNNIDKIDFKWLLKFKRILKLIHRKSHPRKQKLNLI----KKFVNRLKKWNWPDLIKNIDE 1851 WNW LI+N + D+ +K+ I+ + + VK + K +D NL K+ + I++ + K + D+ ++ I+ +V+W ++++K+ +K + +K+DL L ++ LI +R K + K + + V L KW W +L+K + +E V K + + ++K +F++I+ L ++ + + ++++ I IK +K ++ +LKLI+ +W+++INE +GID+ +W R NKL LI+L + +KID+ WL +F +I R K I K K ++ ++ L +WNW +L+ +++EK++EN+ KEIEL+ +++KE F EI+ +L KR +VIFK +RKIK K P +IKV KE LL K+KLIID+NW +IN +G+DKVKWT R NKLIQ+I+ N DKID KWL KF R++K++ +K+ R + +I KKFVNRL KWNWPDLIKNIDE Sbjct: 1327 WNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIK---------------NDKRPIIDIKKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKFVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKKPIIDIKV---QKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKFVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKEKLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKFVNRLIKWNWPDLIKNIDE 1799 HSP 4 Score: 198.364 bits (503), Expect = 2.536e-53 Identity = 187/523 (35.76%), Postives = 308/523 (58.89%), Query Frame = 1 Query: 349 VIEQPESETIITT-----VEQVKNWNWEQLIENTKDTDQSTIKRNIKKIFEAVKTSGNKNLDPNLLKKWKSIYEILSKSR--------IHQQFIRTQDQWTELTDEFGNGQLESFISTLDPKLQVEWKDKLSKVKWFVKNKEFNKLDLNSLNEWKNLIYKLRLKIHGKESYKKRLLVQWQSHVXXXXXXXXXXXXXFVSGNYKERVTKTVNELFVIIKRQEFEKINLELVTNVKFVMRLLKRLWR-----IHIKYGSRKKANMIRRLKLILSIDWRRIINELEGIDRNQWIERFNKLKNLIQLKRYNKIDVNWLAEFKPIIDKFRGKIKINYIHQXXXXXXXXXXXXXXXXWNWKNLVVDLXXXXXXXXXXXXXLMERIIKEEHFNEINVDLIKRFKVIFKILRKIKQSKYPKGNXXXXXXXXXXXXXXXXDSNWLNIINGLEGVDKVKWTTRFNKLIQMIRNNNIDKIDFKWLLKFKRILKLIHRKSHPRKQKLNLI----KKFVNRLKKWNWPDLIKNIDE 1851 I++P + IIT ++++ WNW LI+N + D+ +K+ I+ + + VK + K +D NL K+ + I++ + K + I Q + ++ + D + I+ +V+W ++++K+ +K + +K+DL L ++ LI +R K + K + + V L KW W +L+K + +E V K + + ++K +F++I+ L ++ + + ++++ I IK +K ++ +LKLI+ +W+++INE +GID+ +W R NKL LI+L + +KID+ WL +F +I R K I K K ++ ++ L +WNW +L+ +++EK++EN+ KEIEL+ +++KE F EI+ +L KR +VIFK +RKIK K P +IKV KE LL K+KLIID+NW +IN +G+DKVKWT R NKLIQ+I+ N DKID KWL KF R++K++ +K+ R + +I KKFVNRL KWNWPDLIKNIDE Sbjct: 663 AIKKPITVKIITKNVKRFIKRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKEKLLNKLKLIID----TNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKFVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIKV---QKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKFVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKKPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKFVNRLIKWNWPDLIKNIDE 1178 HSP 5 Score: 187.578 bits (475), Expect = 8.884e-50 Identity = 178/490 (36.33%), Postives = 287/490 (58.57%), Query Frame = 1 Query: 406 WNWEQLIENTKDTDQSTIKRNIKKIFEAVKTSGNKNLDPNLLKKWKSIYEILSKSRIHQQ-FIRTQDQWTELTDEFG---NGQLESFISTLDPKLQVEWKDKLSKVKWFVKNKEFNKLDLNSLNEWKNLIYKLRLKIHGKESYKKRLLVQWQSHVXXXXXXXXXXXXXFVSGNYKERVTKTVNELFVIIKRQEFEKINLELVTNVKFVMRLLKRLWRIHIKYGSRKKANMIRRLKLILSIDWRRIINELEGIDRNQWIERFNKLKNLIQLKRYNKIDVNWLAEFKPIIDKFRGKIKINYIHQXXXXXXXXXXXXXXXXWNWKNLVVDLXXXXXXXXXXXXXLMERIIKEEHFNEINVDLIKRFKVIFKILRKIKQSKYPKGNXXXXXXXXXXXXXXXXDSNWLNIINGLEGVDKVKWTTRFNKLIQMIRNNNIDKIDFKWLLKFKRILKLIHRKSHPRKQKLNLI----KKFVNRLKKWNWPDLIKNIDE 1851 WNW LI+N + D+ +K+ I+ + + VK + K +D NL K+ + I++ + K + ++ I + Q L ++ + + I+ +V+W ++++K+ +K + +K+DL L ++ LI +R K + K + + V L KW W +L+K + +E V K + + ++K +F++I+ L ++ + + +++ IK R ID +++INE +GID+ +W R NKL LI+L + +KID+ WL +F +I R K I K K ++ ++ L +WNW +L+ +++EK++EN+ KEIEL+ +++KE F EI+ +L KR +VIFK +RKIK K P +IKV KE LL K+KLIID+NW +IN +G+DKVKWT R NKLIQ+I+ N DKID KWL KF R++K++ +K+ R + +I KKFVNRL KWNWPDLIKNIDE Sbjct: 1167 WNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKFVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRK-----IKNDKRP------------IIDIKKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKFVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKKPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKFVNRLIKWNWPDLIKNIDE 1639 HSP 6 Score: 186.808 bits (473), Expect = 1.518e-49 Identity = 184/500 (36.80%), Postives = 299/500 (59.80%), Query Frame = 1 Query: 406 WNWEQLIENTKDTDQSTIKRNIKKIFEAVKTSGNKNLDPNLLKKWKSIYEILSKSRIHQQ-FIRTQDQWTELTDEFG---NGQLESFISTLDPKLQVEWKDKLSKVKWFVKNKEFNKLDLNSLNEWKNLIYKLRLKIHGKESYKKRLLVQW-----QSHVXXXXXXXXXXXXXFVSGNYKERVTKTVNELFVIIKRQEFEKINLELVTNVKFVMRLLKRLWR-----IHIKYGSRKKANMIRRLKLILSIDWRRIINELEGIDRNQWIERFNKLKNLIQLKRYNKIDVNWLAEFKPIIDKFRGKIKINYIHQXXXXXXXXXXXXXXXXWNWKNLVVDLXXXXXXXXXXXXXLMERIIKEEHFNEINVDLIKRFKVIFKILRKIKQSKYPKGNXXXXXXXXXXXXXXXXDSNWLNIINGLEGVDKVKWTTRFNKLIQMIRNNNIDKIDFKWLLKFKRILKLIHRKSHPRKQKLNLI----KKFVNRLKKWNWPDLIKNIDE 1851 WNW LI+N + D+ +K+ I+ + + VK + K +D NL K+ + I++ + K + ++ I + Q L ++ + + I+ +V+W ++++K+ +K + +K+DL L ++K +I I K + KK + V+ + ++ L KW W +L+K + +E V K + + ++K +F++I+ L ++ + + ++++ I IK K N LKLI+ +W+++INE +GID+ +W R NKL LI+L + +KID+ WL +F +I R K I K K ++ ++ L +WNW +L+ +++EK++EN+ KEIEL+ +++KE F EI+ +L KR +VIFK +RKIK K P +IKV KE LL K+KLIID+NW +IN +G+DKVKWT R NKLIQ+I+ N DKID KWL KF R++K++ +K+ R + +I KKFVNRL KWNWPDLIKNIDE Sbjct: 527 WNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFKRII-----NIVKKNAIKKPITVKIITKNVKRFIKRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKEKLLNK---LKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKFVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKFVNRLIKWNWPDLIKNIDE 1018 HSP 7 Score: 176.792 bits (447), Expect = 4.018e-46 Identity = 177/494 (35.83%), Postives = 289/494 (58.50%), Query Frame = 1 Query: 406 WNWEQLIENTKDTDQSTIKRNIKKIFEAVKTSGNKNLDPNLLKKWKSIYEILSKSRIHQQ-FIRTQDQWTELTDEFG---NGQLESFISTLDPKLQVEWKDKLSKVKWFVKNKEFNKLDLNSLNEWKNLIYKLRLKIHGKESYKKRLLVQWQSHVXXXXXXXXXXXXXFVSGNYKERVTKTVNELFVIIKRQEFEKINLELVTNVKFVMRLLKRLWR-----IHIKYGSRKKANMIRRLKLILSIDWRRIINELEGIDRNQWIERFNKLKNLIQLKRYNKIDVNWLAEFKPIIDKFRGKIKINYIHQXXXXXXXXXXXXXXXXWNWKNLVVDLXXXXXXXXXXXXXLMERIIKEEHFNEINVDLIKRFKVIFKILRKIKQSKYPKGNXXXXXXXXXXXXXXXXDSNWLNIINGLEGVDKVKWTTRFNKLIQMIRNNNIDKIDFKWLLKFKRILKLIHRKSHPRKQKLNL----IKKFVNRLKKWNWPDLIKNID 1848 WNW LI+N + D+ +K+ I+ + + VK + K +D NL K+ + I++ + K + ++ I + Q L ++ + + I+ +V+W ++++K+ +K + +K+DL L ++ LI +R K + K + + V L KW W +L+K + +E V K + + ++K +F++I+ L ++ + + ++++ I IK K N LKLI+ +W+++INE +GID+ +W R NKL LI+L + +KID+ WL +F +I R K I K K ++ ++ L +WNW +L+ +++EK++EN+ KEIEL+ +++KE F EI+ +L KR +VIFK +RKIK K P +IKV KE LL K+KLIID+NW +IN +G+DKVKWT R NKLIQ+I+ N DKID KWL KF +I+ +I + + + + IK+ NRL KWNWPDLIKNID Sbjct: 1468 WNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKKPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKFVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKEKLLNK---LKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKFVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKKPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNKIINVIRKNTTEKPITVKFITKDIKRITNRLIKWNWPDLIKNID 1958 HSP 8 Score: 74.7146 bits (182), Expect = 1.797e-13 Identity = 122/513 (23.78%), Postives = 257/513 (50.10%), Query Frame = 1 Query: 406 WNWEQLIENTKDTDQSTIKRNIKKIFEAVKTSGNKNLDPNLLKKWKSIYEILSKSRIHQQ-FIRTQDQWTELTDEFG---NGQLESFISTLDPKLQVEWKDKLSKVKWFVKNKEFNKLDLNSLNEWKNLIYKLRLKIHGKESYKKRLLVQWQSHVXXXXXXXXXX-----XXXFVSGNYKERVTKTVNELFVIIKRQEFEKINLELVTNVKFVMRLLKR-----LWRIHIKYGSRKKANMIRRLKLILSIDWRRIINELEGIDRNQWIERFNKLKNLIQLKRYNKIDVNWLAEFKPIID-KFRGKIKINYIHQXXXXXXXXXXXXXXXXWNWKNLVVDLXXXXXXXXXXXXXLMERIIKEEHFNEINVDLIKRFKVIFKILRK-----IKQSKYPKGNXXXXXXXXXXXXXXXXDSNWLNIINGLEGVDKVKWTTRFNKLIQMIRNNNIDKID---FKW---LLKFKRILKLIHRKSHP-----RKQKLNLIKKFVNRLKKWNWPDLIKNIDE 1851 WNW LI+N + D+ +K+ I+ + + VK + K +D NL K+ + I++ + K + ++ I + Q L ++ + + I+ +V+W ++++K+ +K + +K+DL L ++ +I +R K + +K + V++ + + ++ L+K + +ER K ++ + ++K +F++I+ L ++ + + +++ L ++ I+ +K N + +LK I++ +W +IINE + IDR +WI+R N + L++L +KID+ WL E + I K + N I K K +L L W+ L+ ++E + + +I+ +++ + + +I++DLI I L+K IK +K + N+K E K I ++++ NW ++ ++ ++++ + I ++N +KID W ++ R +K +H K+ RK + K+VN L + NW +L+K I+E Sbjct: 1788 WNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKKPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNKIINVIR-----KNTTEKPITVKFITKDIKRITNRLIKWNWPDLIKNIDVKERERFQKEIDIVLKLVKENKFKEIDENLYKKIRVIYKKIRKVIKDKLPKVEIQI---QKENSLEKLKPIITKNWAKIINETKNIDRVKWIDRINTIIKLVKLNEVDKIDLTWLKELEKIFKVKEIPSTEENIIDIKTITDKSNNFLITLTELKWEELLKYIDESSRPEVLNKIKTFVTLVENKQYEKIDIDLITWITEIITTLKKAKEVQIKNTKTEEVNLKEISETSNKFIDILVEINWEELVKYIDQSNRLEVINKIKTFITFVKNKQYEKIDTNLINWINHIITIFRQVKEVHIKNEKITNDNRKLITDTSNKYVNFLIELNWEELVKQIEE 2292 HSP 9 Score: 72.7886 bits (177), Expect = 6.211e-13 Identity = 89/390 (22.82%), Postives = 202/390 (51.79%), Query Frame = 1 Query: 409 NWEQLIENTKDTDQSTIKRNIKKIFEAVKTSGNKNLDPNLLKKWKSIYEILSK-SRIHQQFIRTQDQWTELTDEFGNGQLESF--------ISTLDPKLQVEWKDKLSKVKWFVKNKEFNKLDLNSLNEWKNLIYKLRL--KIHGK-ESYKKR----LLVQWQSHVXXXXXXXXXXXXXFVSGNYKERVTKTVNELFVIIKRQEFEKINLELVTNVKFVMRLLKRLWRIHIKYGSRKKANMIRRLKLILSIDWRRIINELEGIDRNQWIERFNKLKNLIQLKRYNKIDVNWLAEFKPIIDKFRG---KIKINYIHQXXXXXXXXXXXXXXXXWNWKNLVVDLXXXXXXXXXXXXXLMERIIKEEHFNEINVDLIKRFKVIFKILRKIKQS 1521 NWE L++ ++ + + + I V+ + +D +L+ +I + L K S++H + + + ++ E N +++ + +D + E DK+ K +VKNKE+ K+D +N ++ L+ IH K E KK ++ + ++ L + KW EL++++ N + + K +NE +K + ++ I+++LV ++ +++ L +I + + ++ ++I+++++ L +W ++ ++G+D + WI R KL ++++ K Y KID +W+ E + II K+K I + I K++ ++ L+ W W++ + ++E ++ + K++ + I++ E F ++ + L+ F+ IF+I KI + Sbjct: 3122 NWEVLVKYIDESSRPEVIKKITTFIALVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHIKNEKIKKENVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVN---YIGQIITTLQKIRVTKFTSRRKDIIKKIQIKLPGNWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFDWIEELQKIISLISEEEIKLKKEVILNEGSIAKLKSNVKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKINDA 3508 HSP 10 Score: 63.1586 bits (152), Expect = 6.428e-10 Identity = 110/518 (21.24%), Postives = 249/518 (48.07%), Query Frame = 1 Query: 409 NWEQLIENTKDTDQSTIKRNIKKIFEAVKTSGNKNLDPNLLKKWKSIYEILSKSR-IHQQFIRTQDQWTELTDEFGNGQL--------ESFISTLDPKLQVEWKDKLSKVKWFVKNKEFNKLDLNSLNEWKNLIYKLR--LKIHGK-----ESYKKRLLVQWQSHVXXXXXXXXXXXXXFVSGNYKERVTKTVNELFVIIKRQEFEKINLELVTNVKFVMRLLKRLWRIHIKYGSRKKANMI-------RRLKLILSIDWRRIINELEGIDRNQWIERFNKLKNLIQLKRYNKID---VNWLAEFKPIIDKFRG-KIKINYIHQXXXXXXXXXXXXXXXXWN---WKNLVVDLXXXXXXXXXXXXXLMERIIKEEHFNEINVDLIKRFKVIFKILRKIKQSKYPKGNXXXXXXXXXXXXXXXXDSNWLNIINGLEGVDKVKWTTRFNKLIQMIRNNNIDKIDFKWLLKFKRILKLIHRKSHPRKQKLNL-------IKKFVNRLKKWNWPDLIKNIDE 1851 NWE L++ ++ + + + I V+ + +D +L+ +I + L K R +H + + ++ + E N + E + +D + E K++ V+NK++ K+D++ +N +I LR ++H K E K + ++ +L++ W L+K++ + + V K + +++ +++EKI+++L+ + ++ L+++ ++H K + N+ + + + +W ++ ++ +R + I++ K ++ K Y KID +N++A+ + + R IK I ++ I+ + + N W+ L+ ++E + I K+I +K ++++ I+VDL+ I L+KI+ +K+ ++ ++KKI++ + NW+ ++ ++GVD W R KL+ M+ + KIDF W+ + ++I+ LI + K+++ L +K V +L++W W + IDE Sbjct: 2950 NWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFIALVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHIKNEKIKKENVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIRVTKFTSR-----RKDIIKKIQIKLPGNWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFDWIEELQKIISLISEEEIKLKKEVILNEGSIAKLKSNVKQLQEWKWESYLAYIDE 3462 HSP 11 Score: 59.3066 bits (142), Expect = 9.102e-9 Identity = 107/448 (23.88%), Postives = 208/448 (46.43%), Query Frame = 1 Query: 604 QWTELTDEFGNGQLESFISTLDPKLQVEWKDKLSKVKWFVKNKEFNKLDLNSLNEWKNLIYKLRL--KIHGKE----SYKKRLLVQWQS-HVXXXXXXXXXXXXXFVSGNYKERVTKTVNELFVIIKRQEFEKINLELVTNVKFVMRLLKRLWRIHIKYGSRKKANMI-------RRLKLILSIDWRRIINELEGIDRNQWIERFNKLKNLIQLKRYNKIDVNWLAEFKPIIDKFRGKIKINYIHQXXXXXXXXXXXXXXXXWN-WKNLVVDLXXXXXXXXXXXXXLMERIIKEEHFNEINVDLIKRFKVIFKILRKIKQ-----SKYPKGNXXXXXXXXXXXXXXXXDSNWLNIINGLEGVDKVKWTTRFNKLIQMIRNNNIDKID---FKWLLKFKRILKLIHRKSHPRKQKLN---------LIKKFVNRLKKWNWPDLIKNIDE 1851 Q+ +L +E + E I +D K + E DK+ FV+NK+F ++D LN +I LR +IH K + K+++ + + +V +L KW EL+K++ + TK + +++ +++EKI+++L+ + ++ L+++ +HIK + N+ + + + ++W ++ ++ R + I++ L+Q K+Y KIDV+ + IID R K+ +H K + + + N + N + +L E++ + I E EI+VDLI I LRK++Q K + N+K E K I + + NW ++ ++ + + + I +++N +KID WL L+ + R+ H + +K+ K++N L++ NW L+K IDE Sbjct: 2444 QYIQLLNEL---KWEELIKYIDAKNRPEVVDKIKTFIKFVQNKQFEQIDTGLLNWITRIITTLREVKQIHIKNEEVTTENKKIITETSNKYVNYLIDLKWEELIKYIDEKNRPEFTKKIKTFITLVQNKQYEKIDVDLINWLTEIIPTLRKIREVHIKNAKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLR---KVRQVHIKNEKVTEENLKTITETSNKYINFLREL----------NWEVLVKYIDESSRPEIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKV-RQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDE 2874 HSP 12 Score: 53.5286 bits (127), Expect = 5.897e-7 Identity = 107/527 (20.30%), Postives = 247/527 (46.87%), Query Frame = 1 Query: 409 NWEQLIENTKDTDQSTIKRNIKKIFEAVKTSGNKNLDPNLLKKWKSIYEILSKSR-IHQQFIRTQDQWTELTDEFGNGQL--------ESFISTLDPKLQVEWKDKLSKVKWFVKNKEFNKLDLNSLNEWKNLIYKLR--LKIHGK-----ESYKKRLLVQWQSHVXXXXXXXXXXXXXFVSGNYKERVTKTVNELFVIIKRQEFEKINLELVTNVKFVMRLLKRLWRIHIKYGSRKKANMI-------RRLKLILSIDWRRIINELEGIDRNQWIERFNKLKNLIQLKRYNKIDVNWLAEFKPIIDKFRGKIKINYIHQXXXXXXXXXXXXXXXXW-------NWKNLVVDLXXXXXXXXXXXXXLMERIIKEEHFNEINVDLIKRFKVIFKILRKIKQ-----SKYPKGNXXXXXXXXXXXXXXXXDSNWLNIINGLEGVDKVKWTTRFNKLIQMIRNNNIDKIDFKWLLKFKRIL------KLIHRKSHP-RKQKLNLI----KKFVNRLKKWNWPDLIKNIDE 1851 NWE L++ ++ + + + I V+ + +D +L+ +I + L K R +H + + ++ + E N + E + +D + E K++ V+NK++ K+D++ +N +I LR ++H K E K + ++ +L++ W L+K++ + + V K + +++ +++EKI+++L+ + ++ L+++ ++HIK + N+ + + + ++W ++ ++ R + I++ L+Q K+Y KIDV+ + IID R +++ ++K + ++ ++ NW+ LV ++E + + K+I +++ + + +I+VDLI I LRK+ Q K + N+K+ E K I + + W ++ ++ ++ + + K I ++N +KID + +IL + IH K+ +K+ +++I +K++ L + W +L++ IDE Sbjct: 2778 NWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFIALVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHIKNEKIKKENVDVIIKTSEKYIVMLNELKWEELMQYIDE 3304 The following BLAST results are available for this feature:
BLAST of SMED30000295 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30000295 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30000295 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30000295 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30000295 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30000295 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30000295 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30000295 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 3
BLAST of SMED30000295 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30000295 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30000295 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30000295 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30000295 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30000295 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 2
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30000295 ID=SMED30000295|Name=SMED30000295|organism=Schmidtea mediterranea sexual|type=transcript|length=1851bpback to top protein sequence of SMED30000295-orf-1 >SMED30000295-orf-1 ID=SMED30000295-orf-1|Name=SMED30000295-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=611bp MLKKIILLFLVTWSTLSADNPYNGFETICIPQKACYFVSKFKTSWQTAKEback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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