Intersectin-1
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30000291 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 6
Homology
BLAST of Intersectin-1 vs. Ensembl Human
Match: ITSN1 (intersectin 1 [Source:HGNC Symbol;Acc:HGNC:6183]) HSP 1 Score: 600.512 bits (1547), Expect = 0.000e+0 Identity = 400/1142 (35.03%), Postives = 583/1142 (51.05%), Query Frame = 3
Query: 1938 ILKKQKEPKTAVSEKNYNEFDKIGISKNPQLNSMPSNELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSECQ-----WAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIV-------TSEDLANTESAKFAEGV------PVEEMSESI-IDLMNASQHSAISLNS-----VTSNPYSTISDVENNSALYYPQNTVPV-NSNGAST----------PEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGD--HLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLA--TPCYNTGGMPFVFPLETSSKDKMQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMF 5240
+ + +EP + ++ +K ++ + Q N ++VYY A+Y FE R HDEIT + GDI++++ EP GWL G L + G FP Y K+ P E V + S+ L + TP SSE WA+ W +N E P N D W + + + G+ + + P T + +P G E + + L A YP++A LN D+I VL+Q+ WW + +G FP +YVK++ TS D ++ES + V PV E I + +S+ ++ VT + + A +P N V + +S G+ T PEIAQV+ + EQ+ L GQLI IR+K+P GWW+GE+Q RG++R+IGWFP YV + ++ TS I T + +I MY+Y+AQN DEL+F+K I+ +++K +P+WW G ++ Q GLFP NYV+ + S + W D + +LT E++RQ YI ELI TE+ Y DLQ V ++F +P+ +S LLT E+ IF+NW +LI + AL++RK ++G K P++ +GDIL + P+ Y+RFCS Q G A +Q+K P FKE + + RC+ MP++S+ LKPMQR+T+Y L+++NIL++T +NHPD S ++ A+ A LC VNEGVRE+ENS+RLEW+Q H+QCE LSE+L FNS TNCLG RK +HSG L K KS KEL GFLFNDF LL T + G VF S K +Q+KMYK PI LN+V++ + S F + I V + LRA S N+R WV+++ AS YI TE KRE + +R R G+GRL+V VVEGI L+ KS+P+CE ++G+Q H TK + TLNPKWN+ QF I++++Q+VLC+TV+ +D F+P+DFLGR E+ V ++ ++ ++ P+ K L L EV GE+VVRLDL +F
Sbjct: 708 LFHQHQEPAKPAVQAPWSTAEKGPLTISAQENV----KVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEP--GWLGGELKGKT--GWFPANYAEKI---PENEVPAPVKPVTDSTSAPAPKLALRE-TPAPLAVTSSEPSTTPNNWADFSSTWPTSTN----EKPE-------------TDNWDAWAAQPSLTVPSAGQLRQRSAFTPATAT------------GSSPSPVLGQGEKV---------------------EGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWW--FGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKAGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKL--------------------------------LSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGE--VNGQVGLFPSNYVKLTTDMDPSQQ-----------------------WCSD--LHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILSAQLPHMQPYIRFCSRQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSSGTDKVF----SPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRV------YTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELK--PCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQG-SKGPVTKCLLLHEVPTGEIVVRLDLQLF 1713 HSP 2 Score: 203.371 bits (516), Expect = 1.264e-51 Identity = 194/540 (35.93%), Postives = 290/540 (53.70%), Query Frame = 3
Query: 12 A*FPNIFQIDLHMLNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLP--LSLNPLQTN---ATTMSYSGYMPVSNTYNTMPVMHNTSHAMSNNNPNN--TMAVPH-ASAFVNYXXXXXXXXXXXXXXXXNAQF-----GSXXXXXXXXXXSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXK-----KNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAILDEQMRQXXXXXXXXXXXXXXXXXXXXQEKEQARFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKMRLESEKKRLLEMEKRRLEVAIXXXXXXXXXXXXVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYKAGIGSMREHRD 1577
A FP F L ++W IT EE+ K D QF L P++GFITG+QA++ F +S LP VL +IW LAD + DG M+ EF+IAM LIK KL+GY++P LP + P+ + A M MP +P+ MS ++ T AVP A+ + + ++ F GS S+ V + PP+ +W +PQS R +YR FN D+ G LTG +A+ IL+QS LP LASIW+L+DIDQDG LT EEF +AMHL++ A SG+ LP LP +PPS ++ + +S S +P P+ +++ ++K +T ED++R N+ +G ELEKRR L EQ R+E+E ERAE ER E+ RQE+E+ R E+++Q+E +R+ E +REE+ R+ E RE + E E++R LE E+ R + +++R +EQE + + +KK L L +KK L+ +++ + L T + I S + R+
Sbjct: 2 AQFPTPFGGSL------DIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPTLVSSVPTAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDV---ASVPPVA------EWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLE--------DEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRE 518
BLAST of Intersectin-1 vs. Ensembl Human
Match: ITSN1 (intersectin 1 [Source:HGNC Symbol;Acc:HGNC:6183]) HSP 1 Score: 594.349 bits (1531), Expect = 1.252e-180 Identity = 399/1145 (34.85%), Postives = 583/1145 (50.92%), Query Frame = 3
Query: 1938 ILKKQKEPKTAVSEKNYNEFDKIGISKNPQLNSMPSNELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSG---WLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSECQ-----WAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIV-------TSEDLANTESAKFAEGV------PVEEMSESI-IDLMNASQHSAISLNS-----VTSNPYSTISDVENNSALYYPQNTVPV-NSNGAST----------PEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGD--HLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLA--TPCYNTGGMPFVFPLETSSKDKMQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMF 5240
+ + +EP + ++ +K ++ + Q N ++VYY A+Y FE R HDEIT + GDI+++ + E +G WL G L + G FP Y K+ P E V + S+ L + TP SSE WA+ W +N E P N D W + + + G+ + + P T + +P G E + + L A YP++A LN D+I VL+Q+ WW + +G FP +YVK++ TS D ++ES + V PV E I + +S+ ++ VT + + A +P N V + +S G+ T PEIAQV+ + EQ+ L GQLI IR+K+P GWW+GE+Q RG++R+IGWFP YV + ++ TS I T + +I MY+Y+AQN DEL+F+K I+ +++K +P+WW G ++ Q GLFP NYV+ + S + W D + +LT E++RQ YI ELI TE+ Y DLQ V ++F +P+ +S LLT E+ IF+NW +LI + AL++RK ++G K P++ +GDIL + P+ Y+RFCS Q G A +Q+K P FKE + + RC+ MP++S+ LKPMQR+T+Y L+++NIL++T +NHPD S ++ A+ A LC VNEGVRE+ENS+RLEW+Q H+QCE LSE+L FNS TNCLG RK +HSG L K KS KEL GFLFNDF LL T + G VF S K +Q+KMYK PI LN+V++ + S F + I V + LRA S N+R WV+++ AS YI TE KRE + +R R G+GRL+V VVEGI L+ KS+P+CE ++G+Q H TK + TLNPKWN+ QF I++++Q+VLC+TV+ +D F+P+DFLGR E+ V ++ ++ ++ P+ K L L EV GE+VVRLDL +F
Sbjct: 708 LFHQHQEPAKPAVQAPWSTAEKGPLTISAQENV----KVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKT--GWFPANYAEKI---PENEVPAPVKPVTDSTSAPAPKLALRE-TPAPLAVTSSEPSTTPNNWADFSSTWPTSTN----EKPE-------------TDNWDAWAAQPSLTVPSAGQLRQRSAFTPATAT------------GSSPSPVLGQGEKV---------------------EGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWW--FGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKAGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKL--------------------------------LSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGE--VNGQVGLFPSNYVKLTTDMDPSQQ-----------------------WCSD--LHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILSAQLPHMQPYIRFCSRQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSSGTDKVF----SPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRV------YTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELK--PCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQG-SKGPVTKCLLLHEVPTGEIVVRLDLQLF 1718 HSP 2 Score: 202.986 bits (515), Expect = 1.357e-51 Identity = 194/540 (35.93%), Postives = 290/540 (53.70%), Query Frame = 3
Query: 12 A*FPNIFQIDLHMLNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLP--LSLNPLQTN---ATTMSYSGYMPVSNTYNTMPVMHNTSHAMSNNNPNN--TMAVPH-ASAFVNYXXXXXXXXXXXXXXXXNAQF-----GSXXXXXXXXXXSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXK-----KNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAILDEQMRQXXXXXXXXXXXXXXXXXXXXQEKEQARFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKMRLESEKKRLLEMEKRRLEVAIXXXXXXXXXXXXVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYKAGIGSMREHRD 1577
A FP F L ++W IT EE+ K D QF L P++GFITG+QA++ F +S LP VL +IW LAD + DG M+ EF+IAM LIK KL+GY++P LP + P+ + A M MP +P+ MS ++ T AVP A+ + + ++ F GS S+ V + PP+ +W +PQS R +YR FN D+ G LTG +A+ IL+QS LP LASIW+L+DIDQDG LT EEF +AMHL++ A SG+ LP LP +PPS ++ + +S S +P P+ +++ ++K +T ED++R N+ +G ELEKRR L EQ R+E+E ERAE ER E+ RQE+E+ R E+++Q+E +R+ E +REE+ R+ E RE + E E++R LE E+ R + +++R +EQE + + +KK L L +KK L+ +++ + L T + I S + R+
Sbjct: 2 AQFPTPFGGSL------DIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPTLVSSVPTAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDV---ASVPPVA------EWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLE--------DEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRE 518
BLAST of Intersectin-1 vs. Ensembl Human
Match: ITSN2 (intersectin 2 [Source:HGNC Symbol;Acc:HGNC:6184]) HSP 1 Score: 523.087 bits (1346), Expect = 2.032e-155 Identity = 347/1008 (34.42%), Postives = 524/1008 (51.98%), Query Frame = 3
Query: 2430 NQLSFETGQLIKIIDTNLDD--WWTGETTALDANGKEKIIMGWFPKTYV---------RLLDEQNANHPDSGTVTPTAGSLEDLAS------------KYSSLNLSVKYD-------------LNTFDGS----QKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGV---PVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALY----------YPQNTV---------PVNSNGAST--PEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYN---TGGMPFVFPLETSSKDKMQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMFKK 5246
+++SF +G +I++ + + + W G + N GWFP YV + + + A P + +++ T+ S E L+S +S+L ++ + ++ G + L A + A LN DII VL+Q+++WW + +G FP +YVKI+ ++ E V P + + +S++ +T I + + + +P N V + +GAS PEIAQV + +EQ+ L GQLI I +K+ SGWW+GE+Q RG++R+ GWFP +V ++ SS +T + P +I MY+Y+A N DELSF K ++ +++K++P+WW G ++ GLFP NYV+ + S + W D T + + E++RQ YI ELI+TE+ Y DLQ V +VF + + +S LT E+ IF+NW +LI ++ AL++RK G K P+Q +GDIL + + Y+RFCS Q G A LQ+K FKE + S+ RC+ MP++S+ LKPMQRIT+Y LL+ +IL++T ++H D S ++ A+ A LC VNEGVRE+ENS+RLEW+Q H+QCE L+E+L FNS TNCLG RKL+HSG L K KS KEL GFLFNDF LL + G +F SSK QFKMYK PI LN+V++ + SS F + I V + LR + N+R WV+++ AS YI+TE KRE + R +T+G+GRL+V V+E L+ + + KS+P+CE S+G+Q + T+ + TLNPKWN QF IK++ QDVLCLT++++D F+P+DFLGR E+ V + TE+ ++ P+ +RL L EV GE+ VR DL +F++
Sbjct: 757 DEMSFNSGDIIQVDEKTVGEPGWLYG---SFQGN------FGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWW--FGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVNKKPTSAAYSVGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLL----------------------------------GPSSERATPAFH---PVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGE--INGVTGLFPSNYVKMTTDSDPSQQ-----------------------WCAD-LQTLDT-MQPIERKRQGYIHELIQTEERYMADLQLVVEVFQKRMAESGFLTEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPESHADHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLF----SSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRV------YTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELK--ACKPNGKSNPYCEISMGSQSYTTRTIQDTLNPKWNFNCQFFIKDLYQDVLCLTLFDRDQFSPDDFLGRTEIPVAKIRTEQE-SKGPMTRRLLLHEVPTGEVWVRFDLQLFEQ 1676 HSP 2 Score: 192.586 bits (488), Expect = 2.221e-48 Identity = 185/536 (34.51%), Postives = 284/536 (52.99%), Query Frame = 3
Query: 33 QIDLHMLNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLP--LSLNPLQTNATTMSYS-GYMPVSNTYNTMP---VMHNTSHAMSNNN-------------------PNNT------MAVPHASAFVNYXXXXXXXXXX-XXXXXXNAQFGSXXXXXXXXXXSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXKKNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAILDEQMRQXXXXXXXXXXXXXXXXXXXXQEKEQARFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKMRLESEKKRLLEMEKRRLEVAIXXXXXXXXXXXXVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYK 1544
Q M PN+W ITSEE+ K D QF L P G+ITG+QA++ F +S LP VL IW L+D +KDG M+ EF+IAM LIK KL+G ++P VLP + P+ + + + G MP + +P + + S A S N PN T + +P++S+ + + +S ++ S AQ S ++ S N P T +W +PQ R +YR +FN D++ G+L+G +A+ L+QS L T LA+IW+LAD+D DG L EEF +AMHL + A++G+ LP LP L S I+ I NG + E+E Q+K +T ED+R+ANY +G ELEKRR L EQ ++E E + KE+ E ER ++ QE+E + E+++++E +R+ E +REE+ R+ E RE + E E++R LE E+ R + ++++ +EQE + +NSKK NL L K + + R++ ++ +T K
Sbjct: 14 QFPTAMNGGPNMWAITSEERTKHDRQFDNLKPSGGYITGDQARNFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQPLPPAAPITSLSSATSGTNLPPLMMPTPLVPSVSTSSLPNGTASLIQPLPIPYSSSTLPHGSSYSLMMGGFGGASIQKAQ--SLIDLGSSSSTSSTASLSGNSPKTGT----SEWAVPQPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADVDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPEL------------VPPSFRGGKQIDSI-NGTLPSYQKMQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEWERIRRQELLNQKNREQEEIVRLNSKKKNLHLELEALNGKHQQISGRLQDVRLKKQTQK 530 HSP 3 Score: 98.2117 bits (243), Expect = 1.530e-19 Identity = 118/480 (24.58%), Postives = 196/480 (40.83%), Query Frame = 3
Query: 2052 LVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSG--------------PP-----STESTQTKIDVNQNISS------GFADL-----------FNNDITPKGTLRKSSECQW-----AEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLN---LSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVP-VEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSA----------------LYYPQNTVPV--NSNGASTPE---IAQVVIEFNPLS--AEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYV 3287
LV Y A+Y FE R HDE++F +GDII ++ + EP GWLYG + G FP YV K+ PP +T ++ + NQ S F++L F ++P + Q A+A+ W + + N L+F +I +++ ++WW GE G+ GWFPK+YV+++ P S E+ + Y+++N S Y + G + +A YPY + GDL G+ I V + +WW G + G+FPSNYVK E + + + P + +++ + + AS +SL P I ++ N++ ++P + V + S+ +TP + QV+ ++ + +++ GQLI + K WW+GEI FP YV
Sbjct: 742 LVNYRALYPFEARNHDEMSFNSGDIIQVDEKTVGEP--GWLYGSFQG--NFGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKD-------NHLNFSKHDIITVLEQQ-ENWWFGEVHG----GR-----GWFPKSYVKII-------PGSEV------KREEPEALYAAVNKKPTSAAYSV----GEE---YIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTG--SIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYV----ASGSEQLSL-----APGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATPAFHPVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGEINGVTGL-----FPSNYV 1164
BLAST of Intersectin-1 vs. Ensembl Human
Match: ITSN2 (intersectin 2 [Source:HGNC Symbol;Acc:HGNC:6184]) HSP 1 Score: 522.316 bits (1344), Expect = 3.625e-155 Identity = 347/1008 (34.42%), Postives = 524/1008 (51.98%), Query Frame = 3
Query: 2430 NQLSFETGQLIKIIDTNLDD--WWTGETTALDANGKEKIIMGWFPKTYV---------RLLDEQNANHPDSGTVTPTAGSLEDLAS------------KYSSLNLSVKYD-------------LNTFDGS----QKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGV---PVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALY----------YPQNTV---------PVNSNGAST--PEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYN---TGGMPFVFPLETSSKDKMQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMFKK 5246
+++SF +G +I++ + + + W G + N GWFP YV + + + A P + +++ T+ S E L+S +S+L ++ + ++ G + L A + A LN DII VL+Q+++WW + +G FP +YVKI+ ++ E V P + + +S++ +T I + + + +P N V + +GAS PEIAQV + +EQ+ L GQLI I +K+ SGWW+GE+Q RG++R+ GWFP +V ++ SS +T + P +I MY+Y+A N DELSF K ++ +++K++P+WW G ++ GLFP NYV+ + S + W D T + + E++RQ YI ELI+TE+ Y DLQ V +VF + + +S LT E+ IF+NW +LI ++ AL++RK G K P+Q +GDIL + + Y+RFCS Q G A LQ+K FKE + S+ RC+ MP++S+ LKPMQRIT+Y LL+ +IL++T ++H D S ++ A+ A LC VNEGVRE+ENS+RLEW+Q H+QCE L+E+L FNS TNCLG RKL+HSG L K KS KEL GFLFNDF LL + G +F SSK QFKMYK PI LN+V++ + SS F + I V + LR + N+R WV+++ AS YI+TE KRE + R +T+G+GRL+V V+E L+ + + KS+P+CE S+G+Q + T+ + TLNPKWN QF IK++ QDVLCLT++++D F+P+DFLGR E+ V + TE+ ++ P+ +RL L EV GE+ VR DL +F++
Sbjct: 747 DEMSFNSGDIIQVDEKTVGEPGWLYG---SFQGN------FGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWW--FGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVNKKPTSAAYSVGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLL----------------------------------GPSSERATPAFH---PVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGE--INGVTGLFPSNYVKMTTDSDPSQQ-----------------------WCAD-LQTLDT-MQPIERKRQGYIHELIQTEERYMADLQLVVEVFQKRMAESGFLTEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPESHADHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLF----SSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRV------YTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELK--ACKPNGKSNPYCEISMGSQSYTTRTIQDTLNPKWNFNCQFFIKDLYQDVLCLTLFDRDQFSPDDFLGRTEIPVAKIRTEQE-SKGPMTRRLLLHEVPTGEVWVRFDLQLFEQ 1666 HSP 2 Score: 192.586 bits (488), Expect = 2.526e-48 Identity = 185/536 (34.51%), Postives = 284/536 (52.99%), Query Frame = 3
Query: 33 QIDLHMLNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLP--LSLNPLQTNATTMSYS-GYMPVSNTYNTMP---VMHNTSHAMSNNN-------------------PNNT------MAVPHASAFVNYXXXXXXXXXX-XXXXXXNAQFGSXXXXXXXXXXSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXKKNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAILDEQMRQXXXXXXXXXXXXXXXXXXXXQEKEQARFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKMRLESEKKRLLEMEKRRLEVAIXXXXXXXXXXXXVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYK 1544
Q M PN+W ITSEE+ K D QF L P G+ITG+QA++ F +S LP VL IW L+D +KDG M+ EF+IAM LIK KL+G ++P VLP + P+ + + + G MP + +P + + S A S N PN T + +P++S+ + + +S ++ S AQ S ++ S N P T +W +PQ R +YR +FN D++ G+L+G +A+ L+QS L T LA+IW+LAD+D DG L EEF +AMHL + A++G+ LP LP L S I+ I NG + E+E Q+K +T ED+R+ANY +G ELEKRR L EQ ++E E + KE+ E ER ++ QE+E + E+++++E +R+ E +REE+ R+ E RE + E E++R LE E+ R + ++++ +EQE + +NSKK NL L K + + R++ ++ +T K
Sbjct: 4 QFPTAMNGGPNMWAITSEERTKHDRQFDNLKPSGGYITGDQARNFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQPLPPAAPITSLSSATSGTNLPPLMMPTPLVPSVSTSSLPNGTASLIQPLPIPYSSSTLPHGSSYSLMMGGFGGASIQKAQ--SLIDLGSSSSTSSTASLSGNSPKTGT----SEWAVPQPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADVDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPEL------------VPPSFRGGKQIDSI-NGTLPSYQKMQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEWERIRRQELLNQKNREQEEIVRLNSKKKNLHLELEALNGKHQQISGRLQDVRLKKQTQK 520 HSP 3 Score: 97.8265 bits (242), Expect = 1.870e-19 Identity = 118/480 (24.58%), Postives = 196/480 (40.83%), Query Frame = 3
Query: 2052 LVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSG--------------PP-----STESTQTKIDVNQNISS------GFADL-----------FNNDITPKGTLRKSSECQW-----AEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLN---LSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVP-VEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSA----------------LYYPQNTVPV--NSNGASTPE---IAQVVIEFNPLS--AEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYV 3287
LV Y A+Y FE R HDE++F +GDII ++ + EP GWLYG + G FP YV K+ PP +T ++ + NQ S F++L F ++P + Q A+A+ W + + N L+F +I +++ ++WW GE G+ GWFPK+YV+++ P S E+ + Y+++N S Y + G + +A YPY + GDL G+ I V + +WW G + G+FPSNYVK E + + + P + +++ + + AS +SL P I ++ N++ ++P + V + S+ +TP + QV+ ++ + +++ GQLI + K WW+GEI FP YV
Sbjct: 732 LVNYRALYPFEARNHDEMSFNSGDIIQVDEKTVGEP--GWLYGSFQG--NFGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKD-------NHLNFSKHDIITVLEQQ-ENWWFGEVHG----GR-----GWFPKSYVKII-------PGSEV------KREEPEALYAAVNKKPTSAAYSV----GEE---YIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTG--SIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYV----ASGSEQLSL-----APGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATPAFHPVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGEINGVTGL-----FPSNYV 1154
BLAST of Intersectin-1 vs. Ensembl Human
Match: ITSN2 (intersectin 2 [Source:HGNC Symbol;Acc:HGNC:6184]) HSP 1 Score: 521.931 bits (1343), Expect = 8.224e-155 Identity = 347/1008 (34.42%), Postives = 524/1008 (51.98%), Query Frame = 3
Query: 2430 NQLSFETGQLIKIIDTNLDD--WWTGETTALDANGKEKIIMGWFPKTYV---------RLLDEQNANHPDSGTVTPTAGSLEDLAS------------KYSSLNLSVKYD-------------LNTFDGS----QKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGV---PVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALY----------YPQNTV---------PVNSNGAST--PEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYN---TGGMPFVFPLETSSKDKMQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMFKK 5246
+++SF +G +I++ + + + W G + N GWFP YV + + + A P + +++ T+ S E L+S +S+L ++ + ++ G + L A + A LN DII VL+Q+++WW + +G FP +YVKI+ ++ E V P + + +S++ +T I + + + +P N V + +GAS PEIAQV + +EQ+ L GQLI I +K+ SGWW+GE+Q RG++R+ GWFP +V ++ SS +T + P +I MY+Y+A N DELSF K ++ +++K++P+WW G ++ GLFP NYV+ + S + W D T + + E++RQ YI ELI+TE+ Y DLQ V +VF + + +S LT E+ IF+NW +LI ++ AL++RK G K P+Q +GDIL + + Y+RFCS Q G A LQ+K FKE + S+ RC+ MP++S+ LKPMQRIT+Y LL+ +IL++T ++H D S ++ A+ A LC VNEGVRE+ENS+RLEW+Q H+QCE L+E+L FNS TNCLG RKL+HSG L K KS KEL GFLFNDF LL + G +F SSK QFKMYK PI LN+V++ + SS F + I V + LR + N+R WV+++ AS YI+TE KRE + R +T+G+GRL+V V+E L+ + + KS+P+CE S+G+Q + T+ + TLNPKWN QF IK++ QDVLCLT++++D F+P+DFLGR E+ V + TE+ ++ P+ +RL L EV GE+ VR DL +F++
Sbjct: 774 DEMSFNSGDIIQVDEKTVGEPGWLYG---SFQGN------FGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWW--FGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVNKKPTSAAYSVGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLL----------------------------------GPSSERATPAFH---PVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGE--INGVTGLFPSNYVKMTTDSDPSQQ-----------------------WCAD-LQTLDT-MQPIERKRQGYIHELIQTEERYMADLQLVVEVFQKRMAESGFLTEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPESHADHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLF----SSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRV------YTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELK--ACKPNGKSNPYCEISMGSQSYTTRTIQDTLNPKWNFNCQFFIKDLYQDVLCLTLFDRDQFSPDDFLGRTEIPVAKIRTEQE-SKGPMTRRLLLHEVPTGEVWVRFDLQLFEQ 1693 HSP 2 Score: 192.586 bits (488), Expect = 2.464e-48 Identity = 185/536 (34.51%), Postives = 284/536 (52.99%), Query Frame = 3
Query: 33 QIDLHMLNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLP--LSLNPLQTNATTMSYS-GYMPVSNTYNTMP---VMHNTSHAMSNNN-------------------PNNT------MAVPHASAFVNYXXXXXXXXXX-XXXXXXNAQFGSXXXXXXXXXXSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXKKNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAILDEQMRQXXXXXXXXXXXXXXXXXXXXQEKEQARFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKMRLESEKKRLLEMEKRRLEVAIXXXXXXXXXXXXVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYK 1544
Q M PN+W ITSEE+ K D QF L P G+ITG+QA++ F +S LP VL IW L+D +KDG M+ EF+IAM LIK KL+G ++P VLP + P+ + + + G MP + +P + + S A S N PN T + +P++S+ + + +S ++ S AQ S ++ S N P T +W +PQ R +YR +FN D++ G+L+G +A+ L+QS L T LA+IW+LAD+D DG L EEF +AMHL + A++G+ LP LP L S I+ I NG + E+E Q+K +T ED+R+ANY +G ELEKRR L EQ ++E E + KE+ E ER ++ QE+E + E+++++E +R+ E +REE+ R+ E RE + E E++R LE E+ R + ++++ +EQE + +NSKK NL L K + + R++ ++ +T K
Sbjct: 4 QFPTAMNGGPNMWAITSEERTKHDRQFDNLKPSGGYITGDQARNFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQPLPPAAPITSLSSATSGTNLPPLMMPTPLVPSVSTSSLPNGTASLIQPLPIPYSSSTLPHGSSYSLMMGGFGGASIQKAQ--SLIDLGSSSSTSSTASLSGNSPK----TGTSEWAVPQPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADVDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPEL------------VPPSFRGGKQIDSI-NGTLPSYQKMQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEWERIRRQELLNQKNREQEEIVRLNSKKKNLHLELEALNGKHQQISGRLQDVRLKKQTQK 520 HSP 3 Score: 97.8265 bits (242), Expect = 1.928e-19 Identity = 118/480 (24.58%), Postives = 196/480 (40.83%), Query Frame = 3
Query: 2052 LVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSG--------------PP-----STESTQTKIDVNQNISS------GFADL-----------FNNDITPKGTLRKSSECQW-----AEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLN---LSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVP-VEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSA----------------LYYPQNTVPV--NSNGASTPE---IAQVVIEFNPLS--AEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYV 3287
LV Y A+Y FE R HDE++F +GDII ++ + EP GWLYG + G FP YV K+ PP +T ++ + NQ S F++L F ++P + Q A+A+ W + + N L+F +I +++ ++WW GE G+ GWFPK+YV+++ P S E+ + Y+++N S Y + G + +A YPY + GDL G+ I V + +WW G + G+FPSNYVK E + + + P + +++ + + AS +SL P I ++ N++ ++P + V + S+ +TP + QV+ ++ + +++ GQLI + K WW+GEI FP YV
Sbjct: 759 LVNYRALYPFEARNHDEMSFNSGDIIQVDEKTVGEP--GWLYGSFQG--NFGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKD-------NHLNFSKHDIITVLEQQ-ENWWFGEVHG----GR-----GWFPKSYVKII-------PGSEV------KREEPEALYAAVNKKPTSAAYSV----GEE---YIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTG--SIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYV----ASGSEQLSL-----APGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATPAFHPVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGEINGVTGL-----FPSNYV 1181
BLAST of Intersectin-1 vs. Ensembl Celegans
Match: ehs-1 (EHS-1; Eps15 (Endocytosis protein) Homologous Sequence [Source:UniProtKB/TrEMBL;Acc:Q9BIF4]) HSP 1 Score: 113.62 bits (283), Expect = 5.438e-25 Identity = 78/279 (27.96%), Postives = 130/279 (46.59%), Query Frame = 3
Query: 60 PNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLPLSLNPLQTNATTMSYSGYMP--VSNTYNTMPVMHNTSHAMSNNNPNNTMAVPHASAFVNYXXXXXXXXXXXXXXXXNAQFGSXXXXXXXXXXSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSG 890
P W IT ++ K+D FQ LNPVNG ++G + + S L L RIW L+D DKDG ++ E ++A+HL+ R L+ D +P L P + + Y+ P + + P+M + + ++++ + VN S S + + + + P + IH+ + + W I Y QF Q D NK G + G++ +A ++ +GL LA +W+LADI + G L E+F + MHL++ A+ G
Sbjct: 153 PPQWPITPTDQAKYDSIFQSLNPVNGKLSGAHVRPVLMNSGLDAHALARIWELSDQDKDGNLDRIEMSVALHLVYRSLQS----DPVPAQLPPNLIHPSKAMYAHSSPNFAAPPHPPRPMMGSRAGSVTSLDD------------VNMSQSYSATMPRAYPPQPGRAYSAQAHVNGSRTSGASTPISASHS-IHSFPAGE-WPINTG---DYADQFAQTDTNKDGLVDGMDMRAPMMTTGLSAQILAHVWALADIKKCGQLNLEQFALTMHLLDMAKRG 410 HSP 2 Score: 51.9878 bits (123), Expect = 4.845e-6 Identity = 23/69 (33.33%), Postives = 35/69 (50.72%), Query Frame = 3
Query: 132 NGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLPLSLNP 338
+G + G ++ + L +L +W LAD K G +N +FA+ MHL+ G +P LPL L P
Sbjct: 355 DGLVDGMDMRAPMMTTGLSAQILAHVWALADIKKCGQLNLEQFALTMHLLDMAKRGESIPSELPLHLIP 423
BLAST of Intersectin-1 vs. Ensembl Celegans
Match: itsn-1 (ITSN (Intersectin) family [Source:UniProtKB/TrEMBL;Acc:E6N0W1]) HSP 1 Score: 100.523 bits (249), Expect = 3.894e-21 Identity = 109/472 (23.09%), Postives = 184/472 (38.98%), Query Frame = 3
Query: 2430 NQLSFETGQLIKIIDTNLDD--WWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYD--------LNTFDGSQKLL------------CVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTE---------------------------SAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALY----------YPQNTVPVNSNGASTPEI----------------AQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMG-RNLLSSQEGLFPVNYVRR 3617
++LSFE G +I + ++ + W G+ +EK+ GWFP+ +V + A P G P ++ S + VK + +G C+A++ ++A DL+ GD IEVL++++ WKG N G FP +YVK V + T + + +E P +++ I D A+ + + TI +E N + +P N V ++ A P A+VV++F + Q+ ++VG++++IREKS +GWW+GE+ + G+ WFPG+YV + ++ +S P +Y+Y A DEL F ++++ DK+E EWW G R S+ GLFP NYV++
Sbjct: 227 DELSFEPGDVIIVFQSHAAEPGWRAGQL-------REKV--GWFPEAFVEAI----AAVPTPGGDPPIQNMPPNMTPSSSVDQIGVKAARKAEIAAAMGLTEGGAPPASSAPAAAAVISQCIAQFQWRARNEEDLSFAKGDTIEVLEKQEMKWKGRN-PAGEIGWFPKSYVKEVGATTSTTTPIVSPSKASAGAPGAAAGAQYDVVPSDVTLQASETAPQQQLYTVIYDF------EAVETTDLALHVGDTILVLEKNDEWWKGRCNGREGIFPANYVEISVQQAGDPTPPTQAPTPAAPPTVLCEAKVVVDFVASAPNQLGIKVGEIVKIREKSAAGWWEGELIRNGKPIAG-WFPGEYVK---------------------------------VLEEAAS-----------PATRATAVYDYEASQPDELGFKTGDVIIVTDKSEAEWWSGHREQDPSKSGLFPSNYVQQ 633 HSP 2 Score: 88.5817 bits (218), Expect = 2.427e-17 Identity = 101/449 (22.49%), Postives = 172/449 (38.31%), Query Frame = 3
Query: 2067 AIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSG-------------PPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKS---------SECQWAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFD--------GSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSE----------DLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNS--ALYYPQNTVPVNSNGASTPEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYV 3287
A++AFE R DE++F+ GD+II+ EP GW G L G FPE +V ++ PP+ + + + + +T G S S+C A + W+ + LSF G I++++ + W G A + +GWFPK+YV+ + + T TP S ++ +YD+ D Q+ L Y ++A DL + GD I VL++ +WWKG R +G+FP+NYV+I + E V + S + + + + ++ + + N A ++P V V AS A V ++ +++ + G +I + +KS + WW G +Q + + FP YV
Sbjct: 217 ALFAFEARSEDELSFEPGDVIIVFQSHAAEP--GWRAGQL--REKVGWFPEAFVEAIAAVPTPGGDPPIQNMPPNMTPSSSVDQIGVKAARKAEIAAAMGLTEGGAPPASSAPAAAAVISQCI---AQFQWRARNE-------EDLSFAKGDTIEVLEKQ-EMKWKGRNPAGE--------IGWFPKSYVKEVG------ATTSTTTPIV-SPSKASAGAPGAAAGAQYDVVPSDVTLQASETAPQQQLYTVIYDFEAVETTDLALHVGDTILVLEKNDEWWKG--RCNGREGIFPANYVEISVQQAGDPTPPTQAPTPAAPPTVLCEAKVVVDFVASAPNQLGIKVGEIVKIREKSAAGWWEGELIRNGKPIAGWFPGEYVKVLEEAASPATRATAVYDYEASQPDELGFKTGDVIIVTDKSEAEWWSGHREQDPSKSGL--FPSNYV 631
BLAST of Intersectin-1 vs. Ensembl Celegans
Match: itsn-1 (ITSN (Intersectin) family [Source:UniProtKB/TrEMBL;Acc:E6N0W1]) HSP 1 Score: 100.523 bits (249), Expect = 4.110e-21 Identity = 109/472 (23.09%), Postives = 184/472 (38.98%), Query Frame = 3
Query: 2430 NQLSFETGQLIKIIDTNLDD--WWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYD--------LNTFDGSQKLL------------CVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTE---------------------------SAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALY----------YPQNTVPVNSNGASTPEI----------------AQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMG-RNLLSSQEGLFPVNYVRR 3617
++LSFE G +I + ++ + W G+ +EK+ GWFP+ +V + A P G P ++ S + VK + +G C+A++ ++A DL+ GD IEVL++++ WKG N G FP +YVK V + T + + +E P +++ I D A+ + + TI +E N + +P N V ++ A P A+VV++F + Q+ ++VG++++IREKS +GWW+GE+ + G+ WFPG+YV + ++ +S P +Y+Y A DEL F ++++ DK+E EWW G R S+ GLFP NYV++
Sbjct: 225 DELSFEPGDVIIVFQSHAAEPGWRAGQL-------REKV--GWFPEAFVEAI----AAVPTPGGDPPIQNMPPNMTPSSSVDQIGVKAARKAEIAAAMGLTEGGAPPASSAPAAAAVISQCIAQFQWRARNEEDLSFAKGDTIEVLEKQEMKWKGRN-PAGEIGWFPKSYVKEVGATTSTTTPIVSPSKASAGAPGAAAGAQYDVVPSDVTLQASETAPQQQLYTVIYDF------EAVETTDLALHVGDTILVLEKNDEWWKGRCNGREGIFPANYVEISVQQAGDPTPPTQAPTPAAPPTVLCEAKVVVDFVASAPNQLGIKVGEIVKIREKSAAGWWEGELIRNGKPIAG-WFPGEYVK---------------------------------VLEEAAS-----------PATRATAVYDYEASQPDELGFKTGDVIIVTDKSEAEWWSGHREQDPSKSGLFPSNYVQQ 631 HSP 2 Score: 88.5817 bits (218), Expect = 2.354e-17 Identity = 101/449 (22.49%), Postives = 172/449 (38.31%), Query Frame = 3
Query: 2067 AIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSG-------------PPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKS---------SECQWAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFD--------GSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSE----------DLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNS--ALYYPQNTVPVNSNGASTPEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYV 3287
A++AFE R DE++F+ GD+II+ EP GW G L G FPE +V ++ PP+ + + + + +T G S S+C A + W+ + LSF G I++++ + W G A + +GWFPK+YV+ + + T TP S ++ +YD+ D Q+ L Y ++A DL + GD I VL++ +WWKG R +G+FP+NYV+I + E V + S + + + + ++ + + N A ++P V V AS A V ++ +++ + G +I + +KS + WW G +Q + + FP YV
Sbjct: 215 ALFAFEARSEDELSFEPGDVIIVFQSHAAEP--GWRAGQL--REKVGWFPEAFVEAIAAVPTPGGDPPIQNMPPNMTPSSSVDQIGVKAARKAEIAAAMGLTEGGAPPASSAPAAAAVISQCI---AQFQWRARNE-------EDLSFAKGDTIEVLEKQ-EMKWKGRNPAGE--------IGWFPKSYVKEVG------ATTSTTTPIV-SPSKASAGAPGAAAGAQYDVVPSDVTLQASETAPQQQLYTVIYDFEAVETTDLALHVGDTILVLEKNDEWWKG--RCNGREGIFPANYVEISVQQAGDPTPPTQAPTPAAPPTVLCEAKVVVDFVASAPNQLGIKVGEIVKIREKSAAGWWEGELIRNGKPIAGWFPGEYVKVLEEAASPATRATAVYDYEASQPDELGFKTGDVIIVTDKSEAEWWSGHREQDPSKSGL--FPSNYV 629
BLAST of Intersectin-1 vs. Ensembl Celegans
Match: rme-1 (Receptor Mediated Endocytosis [Source:UniProtKB/TrEMBL;Acc:Q86S80]) HSP 1 Score: 97.0561 bits (240), Expect = 7.500e-20 Identity = 43/89 (48.31%), Postives = 60/89 (67.42%), Query Frame = 3
Query: 72 FITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLPLSLNP 338
++ S E+ D F+ L PVNG+++G AK KS LP VLG++W LAD DKDG ++++EFA+A +LI KLEG+E+P LP L P
Sbjct: 679 WVVSRERTTADSTFESLGPVNGYLSGRAAKEHMVKSKLPNSVLGKVWKLADIDKDGQLDADEFALANYLINLKLEGHEIPSELPKHLIP 767 HSP 2 Score: 59.3066 bits (142), Expect = 2.870e-8 Identity = 24/49 (48.98%), Postives = 35/49 (71.43%), Query Frame = 3
Query: 726 GFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLV 872
G+L+G AK +V+S LPN+ L +W LADID+DG L +EF +A +L+
Sbjct: 700 GYLSGRAAKEHMVKSKLPNSVLGKVWKLADIDKDGQLDADEFALANYLI 748
BLAST of Intersectin-1 vs. Ensembl Celegans
Match: rme-1 (Receptor Mediated Endocytosis [Source:UniProtKB/TrEMBL;Acc:Q86S80]) HSP 1 Score: 97.0561 bits (240), Expect = 7.500e-20 Identity = 43/89 (48.31%), Postives = 60/89 (67.42%), Query Frame = 3
Query: 72 FITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLPLSLNP 338
++ S E+ D F+ L PVNG+++G AK KS LP VLG++W LAD DKDG ++++EFA+A +LI KLEG+E+P LP L P
Sbjct: 679 WVVSRERTTADSTFESLGPVNGYLSGRAAKEHMVKSKLPNSVLGKVWKLADIDKDGQLDADEFALANYLINLKLEGHEIPSELPKHLIP 767 HSP 2 Score: 59.3066 bits (142), Expect = 2.870e-8 Identity = 24/49 (48.98%), Postives = 35/49 (71.43%), Query Frame = 3
Query: 726 GFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLV 872
G+L+G AK +V+S LPN+ L +W LADID+DG L +EF +A +L+
Sbjct: 700 GYLSGRAAKEHMVKSKLPNSVLGKVWKLADIDKDGQLDADEFALANYLI 748
BLAST of Intersectin-1 vs. Ensembl Fly
Match: Dap160 (gene:FBgn0023388 transcript:FBtr0301276) HSP 1 Score: 144.436 bits (363), Expect = 3.362e-34 Identity = 125/442 (28.28%), Postives = 207/442 (46.83%), Query Frame = 3
Query: 2433 QLSFETGQLIKI-IDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKY-----------SSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSA-----------ISLNSVTSNPYST---------------ISDVENNSALYYPQN----TVPVNSNGASTP-------EIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYV 3611
+++F G +I + ++ N + W A + NG GWFP++YV L E P + P + +A Y +S +L+ D+ + +A YPY+++ GDL+ G+++ V+ ++ +WW G + G+FPSNYV+ A+ +A A PVE + ++ ++ NA+ +A + + + P S+ +S + S + + P++ + TP EIAQV+ + S EQ+ L GQLI IR+K+ SGWW+GE+Q +G++R+IGWFP YV ++ + N G ++T S IE+ + + D +I +Y Y AQN DELSFDK+ I+ ++ ++EPEWW G L+ GLFP NYV
Sbjct: 692 EITFVPGDIILVPLEQNAEPGWL----AGEINGH----TGWFPESYVEKL-EVGEVAPVAAVEAPVDAQVATVADTYNDNINTSSIPAASADLTAAGDVEYY--------IAAYPYESAEEGDLSFSAGEMVMVIKKEGEWWTGTIGSRT--GMFPSNYVQ------KADVGTASTAAAEPVESLDQTTLN-GNAAYTAAPVEAQEQVYQPLPVQEPSEQPISSPGVGAEEAHEDLDTEVSQINTQSKTQSSEPAESYSRPMSRTSSMTPGMRAKRSEIAQVIAPYEATSTEQLSLTRGQLIMIRKKTDSGWWEGELQAKGRRRQIGWFPATYVKVLQGGR--------NSG------------------RNTPVSGSRIEMTEQIL-DKVIALYPYKAQNDDELSFDKDDIISVLGRDEPEWWRGE--LNGLSGLFPSNYV 1078 HSP 2 Score: 109.383 bits (272), Expect = 2.059e-23 Identity = 121/324 (37.35%), Postives = 191/324 (58.95%), Query Frame = 3
Query: 624 PPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXKKNILGTSMSASMNIPIN-PITNGVSVTXXXXXXXXXXXXXV-LTG----EDERRANYLKGQAELEKRRAILDEQMRQXXXXXXXXXXXXXXXXXXXXQEKEQARFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKMRLESEKKRLLEMEKRRLEVAIXXXXXXXXXXXXVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYKAGIGSMREHRD 1577
P I +V + +W + + + +Y FN DR + G+LTG +A+ +LVQS LP +LA IW+L+DID DG L C+EF +AM L E+A +G ++P LPQ +PP+L+K S+S ++ P + P + SV+ + L G ED+R+ NY+KGQAEL++RR I+++Q R+E E R+ KER E ++ EK R E E+ + E++RQ++ +R+ E+E+EEQ +R E +E R E EK+R E E+ R+ +++ +EQE + + + L L+ EK ++L RI T+ + K I MR RD
Sbjct: 175 PSIESV-NQGEWAVQAAQKRKYTQVFNANDRTRSGYLTGSQARGVLVQSKLPQVTLAQIWTLSDIDGDGRLNCDEFILAMFLCEKAMAGEKIPVTLPQEWVPPNLRKIKSRPGSVSGVVSRPGSQPASRHASVSSQSGVGVVDADPTAGLPGQTSFEDKRKENYVKGQAELDRRRKIMEDQQRKEREERERKEREEADKREKARLEAERKQQEELERQLQRQREIEMEKEEQRKRELEAKEAARKELEKQRQQEWEQARIAEMNAQKEREQERVLKQKAHNTQLNVELSTLNEKIKELSQRICDTRAGVTNVKTVIDGMRTQRD 497 HSP 3 Score: 105.531 bits (262), Expect = 2.952e-22 Identity = 48/90 (53.33%), Postives = 60/90 (66.67%), Query Frame = 3
Query: 63 NLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLPLSL 332
+ W +T E++K+ QF+ L P GF+TG QAK F +S LP +LG+IW LAD D DG MN NEF+IA LI KL G +VP VLP SL
Sbjct: 6 DAWAVTPRERLKYQEQFRALQPQAGFVTGAQAKGFFLQSQLPPLILGQIWALADTDSDGKMNINEFSIACKLINLKLRGMDVPKVLPPSL 95 HSP 4 Score: 77.0258 bits (188), Expect = 1.561e-13 Identity = 53/180 (29.44%), Postives = 83/180 (46.11%), Query Frame = 3
Query: 2052 LVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSECQWAEAIYHW---KPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLD 2582
V Y+A+Y F R +EITF GDII++ + EP GWL G ++ H G FPE YV KL + V+ +++ AD +N++I S++ A + ++ P + G+ LSF G+++ +I +WWTG + G FP YV+ D
Sbjct: 676 FVKYQAVYEFNARNAEEITFVPGDIILVPLEQNAEP--GWLAGEING--HTGWFPESYVEKLEVGEVAPVAAVEAPVDAQVAT-VADTYNDNINTSSIPAASADLTAAGDVEYYIAAYPYESAEEGD----LSFSAGEMVMVIKKE-GEWWTGTIGSR---------TGMFPSNYVQKAD 836 HSP 5 Score: 68.9366 bits (167), Expect = 4.212e-11 Identity = 32/95 (33.68%), Postives = 54/95 (56.84%), Query Frame = 3
Query: 57 NPNLWFITSEEKVKFDVQFQCLNPV-NGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLPLSLNP 338
N W + + +K K+ F + +G++TG+QA+ + +S LP L +IW L+D D DG +N +EF +AM L ++ + G ++P LP P
Sbjct: 181 NQGEWAVQAAQKRKYTQVFNANDRTRSGYLTGSQARGVLVQSKLPQVTLAQIWTLSDIDGDGRLNCDEFILAMFLCEKAMAGEKIPVTLPQEWVP 275 HSP 6 Score: 65.0846 bits (157), Expect = 6.298e-10 Identity = 33/77 (42.86%), Postives = 44/77 (57.14%), Query Frame = 3
Query: 651 DDWVIPQSVRPRYRLQFNQQDRN---KRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLV 872
D W +V PR RL++ +Q R + GF+TG +AK +QS LP L IW+LAD D DG + EF IA L+
Sbjct: 6 DAW----AVTPRERLKYQEQFRALQPQAGFVTGAQAKGFFLQSQLPPLILGQIWALADTDSDGKMNINEFSIACKLI 78 HSP 7 Score: 61.2326 bits (147), Expect = 1.024e-8 Identity = 65/237 (27.43%), Postives = 105/237 (44.30%), Query Frame = 3
Query: 3006 NSVTSNPYSTISDVENNSALYYPQNTVPVNSNGASTPE---IAQVVIEFNPLSAEQIPLQVGQ--LIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSI-NSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVA 3698
+ ++PY+ S+ + SAL P + G PE Q V EFN +AE+I G L+ + + + GW GEI WFP YV + + ++ + + T + IN TSSI ++ +L + ++ I Y Y + +LSF ++++I K E EWW G + S+ G+FP NYV++ G AS A ++ + N N A
Sbjct: 644 GTTVTDPYAVASN--DISALAAPAVDL-----GGPAPEGFVKYQAVYEFNARNAEEITFVPGDIILVPLEQNAEPGWLAGEINGHTG-----WFPESYVEKLEVGEVAPVAAV--EAPVDAQVATVADTYNDNIN--TSSIPAASADLTAAGDVEYYIAAYPYESAEEGDLSFSAGEMVMVI-KKEGEWWTG--TIGSRTGMFPSNYVQKADVGTASTAAAEPVESLDQTTLNGNAA 861
BLAST of Intersectin-1 vs. Ensembl Fly
Match: Dap160 (gene:FBgn0023388 transcript:FBtr0081503) HSP 1 Score: 144.05 bits (362), Expect = 4.898e-34 Identity = 125/442 (28.28%), Postives = 204/442 (46.15%), Query Frame = 3
Query: 2433 QLSFETGQLIKI-IDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKY-----------SSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSA-----------ISLNSVTSNPYST---------------ISDVENNSALYYPQN----TVPVNSNGASTP-------EIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYV 3611
+++F G +I + ++ N + W A + NG GWFP++YV L E P + P + +A Y +S +L+ D+ + +A YPY+++ GDL+ G+++ V+ ++ +WW G + G+FPSNYV+ A+ +A A PVE + + NA+ +A + + + P S+ +S + S + + P++ + TP EIAQV+ + S EQ+ L GQLI IR+K+ SGWW+GE+Q +G++R+IGWFP YV ++ + N G ++T S IE+ + + D +I +Y Y AQN DELSFDK+ I+ ++ ++EPEWW G L+ GLFP NYV
Sbjct: 692 EITFVPGDIILVPLEQNAEPGWL----AGEINGH----TGWFPESYVEKL-EVGEVAPVAAVEAPVDAQVATVADTYNDNINTSSIPAASADLTAAGDVEYY--------IAAYPYESAEEGDLSFSAGEMVMVIKKEGEWWTGTIGSRT--GMFPSNYVQ------KADVGTASTAAAEPVESLDQETTLNGNAAYTAAPVEAQEQVYQPLPVQEPSEQPISSPGVGAEEAHEDLDTEVSQINTQSKTQSSEPAESYSRPMSRTSSMTPGMRAKRSEIAQVIAPYEATSTEQLSLTRGQLIMIRKKTDSGWWEGELQAKGRRRQIGWFPATYVKVLQGGR--------NSG------------------RNTPVSGSRIEMTEQIL-DKVIALYPYKAQNDDELSFDKDDIISVLGRDEPEWWRGE--LNGLSGLFPSNYV 1079 HSP 2 Score: 109.383 bits (272), Expect = 2.169e-23 Identity = 121/324 (37.35%), Postives = 190/324 (58.64%), Query Frame = 3
Query: 624 PPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXKKNILGTSMSASMNIP-INPITNGVSVTXXXXXXXXXXXXXV-LTG----EDERRANYLKGQAELEKRRAILDEQMRQXXXXXXXXXXXXXXXXXXXXQEKEQARFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKMRLESEKKRLLEMEKRRLEVAIXXXXXXXXXXXXVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYKAGIGSMREHRD 1577
P I +V + +W + + + +Y FN DR + G+LTG +A+ +LVQS LP +LA IW+L+DID DG L C+EF +AM L E+A +G ++P LPQ +PP+L+K S+S ++ P P + SV+ + L G ED+R+ NY+KGQAEL++RR I+++Q R+E E R+ KER E ++ EK R E E+ + E++RQ++ +R+ E+E+EEQ +R E +E R E EK+R E E+ R+ +++ +EQE + + + L L+ EK ++L RI T+ + K I MR RD
Sbjct: 175 PSIESV-NQGEWAVQAAQKRKYTQVFNANDRTRSGYLTGSQARGVLVQSKLPQVTLAQIWTLSDIDGDGRLNCDEFILAMFLCEKAMAGEKIPVTLPQEWVPPNLRKIKSRPGSVSGVVSRPGSQPASRHASVSSQSGVGVVDADPTAGLPGQTSFEDKRKENYVKGQAELDRRRKIMEDQQRKEREERERKEREEADKREKARLEAERKQQEELERQLQRQREIEMEKEEQRKRELEAKEAARKELEKQRQQEWEQARIAEMNAQKEREQERVLKQKAHNTQLNVELSTLNEKIKELSQRICDTRAGVTNVKTVIDGMRTQRD 497 HSP 3 Score: 105.531 bits (262), Expect = 3.109e-22 Identity = 48/90 (53.33%), Postives = 60/90 (66.67%), Query Frame = 3
Query: 63 NLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLPLSL 332
+ W +T E++K+ QF+ L P GF+TG QAK F +S LP +LG+IW LAD D DG MN NEF+IA LI KL G +VP VLP SL
Sbjct: 6 DAWAVTPRERLKYQEQFRALQPQAGFVTGAQAKGFFLQSQLPPLILGQIWALADTDSDGKMNINEFSIACKLINLKLRGMDVPKVLPPSL 95 HSP 4 Score: 77.411 bits (189), Expect = 1.222e-13 Identity = 59/215 (27.44%), Postives = 95/215 (44.19%), Query Frame = 3
Query: 2052 LVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSECQWAEAIYHW---KPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKY 2687
V Y+A+Y F R +EITF GDII++ + EP GWL G ++ H G FPE YV KL + V+ +++ AD +N++I S++ A + ++ P + G+ LSF G+++ +I +WWTG + G FP YV+ D GT + A + + ++LN + Y
Sbjct: 676 FVKYQAVYEFNARNAEEITFVPGDIILVPLEQNAEP--GWLAGEING--HTGWFPESYVEKLEVGEVAPVAAVEAPVDAQVAT-VADTYNDNINTSSIPAASADLTAAGDVEYYIAAYPYESAEEGD----LSFSAGEMVMVIKKE-GEWWTGTIGSR---------TGMFPSNYVQ--------KADVGTASTAAAEPVESLDQETTLNGNAAY 863 HSP 5 Score: 68.9366 bits (167), Expect = 4.358e-11 Identity = 31/90 (34.44%), Postives = 53/90 (58.89%), Query Frame = 3
Query: 57 NPNLWFITSEEKVKFDVQFQCLNPV-NGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLP 323
N W + + +K K+ F + +G++TG+QA+ + +S LP L +IW L+D D DG +N +EF +AM L ++ + G ++P LP
Sbjct: 181 NQGEWAVQAAQKRKYTQVFNANDRTRSGYLTGSQARGVLVQSKLPQVTLAQIWTLSDIDGDGRLNCDEFILAMFLCEKAMAGEKIPVTLP 270 HSP 6 Score: 65.0846 bits (157), Expect = 6.628e-10 Identity = 33/77 (42.86%), Postives = 44/77 (57.14%), Query Frame = 3
Query: 651 DDWVIPQSVRPRYRLQFNQQDRN---KRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLV 872
D W +V PR RL++ +Q R + GF+TG +AK +QS LP L IW+LAD D DG + EF IA L+
Sbjct: 6 DAW----AVTPRERLKYQEQFRALQPQAGFVTGAQAKGFFLQSQLPPLILGQIWALADTDSDGKMNINEFSIACKLI 78
BLAST of Intersectin-1 vs. Ensembl Fly
Match: Dap160 (gene:FBgn0023388 transcript:FBtr0333086) HSP 1 Score: 144.05 bits (362), Expect = 5.361e-34 Identity = 125/442 (28.28%), Postives = 204/442 (46.15%), Query Frame = 3
Query: 2433 QLSFETGQLIKI-IDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKY-----------SSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSA-----------ISLNSVTSNPYST---------------ISDVENNSALYYPQN----TVPVNSNGASTP-------EIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYV 3611
+++F G +I + ++ N + W A + NG GWFP++YV L E P + P + +A Y +S +L+ D+ + +A YPY+++ GDL+ G+++ V+ ++ +WW G + G+FPSNYV+ A+ +A A PVE + + NA+ +A + + + P S+ +S + S + + P++ + TP EIAQV+ + S EQ+ L GQLI IR+K+ SGWW+GE+Q +G++R+IGWFP YV ++ + N G ++T S IE+ + + D +I +Y Y AQN DELSFDK+ I+ ++ ++EPEWW G L+ GLFP NYV
Sbjct: 692 EITFVPGDIILVPLEQNAEPGWL----AGEINGH----TGWFPESYVEKL-EVGEVAPVAAVEAPVDAQVATVADTYNDNINTSSIPAASADLTAAGDVEYY--------IAAYPYESAEEGDLSFSAGEMVMVIKKEGEWWTGTIGSRT--GMFPSNYVQ------KADVGTASTAAAEPVESLDQETTLNGNAAYTAAPVEAQEQVYQPLPVQEPSEQPISSPGVGAEEAHEDLDTEVSQINTQSKTQSSEPAESYSRPMSRTSSMTPGMRAKRSEIAQVIAPYEATSTEQLSLTRGQLIMIRKKTDSGWWEGELQAKGRRRQIGWFPATYVKVLQGGR--------NSG------------------RNTPVSGSRIEMTEQIL-DKVIALYPYKAQNDDELSFDKDDIISVLGRDEPEWWRGE--LNGLSGLFPSNYV 1079 HSP 2 Score: 109.383 bits (272), Expect = 2.136e-23 Identity = 121/324 (37.35%), Postives = 190/324 (58.64%), Query Frame = 3
Query: 624 PPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXKKNILGTSMSASMNIP-INPITNGVSVTXXXXXXXXXXXXXV-LTG----EDERRANYLKGQAELEKRRAILDEQMRQXXXXXXXXXXXXXXXXXXXXQEKEQARFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKMRLESEKKRLLEMEKRRLEVAIXXXXXXXXXXXXVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYKAGIGSMREHRD 1577
P I +V + +W + + + +Y FN DR + G+LTG +A+ +LVQS LP +LA IW+L+DID DG L C+EF +AM L E+A +G ++P LPQ +PP+L+K S+S ++ P P + SV+ + L G ED+R+ NY+KGQAEL++RR I+++Q R+E E R+ KER E ++ EK R E E+ + E++RQ++ +R+ E+E+EEQ +R E +E R E EK+R E E+ R+ +++ +EQE + + + L L+ EK ++L RI T+ + K I MR RD
Sbjct: 175 PSIESV-NQGEWAVQAAQKRKYTQVFNANDRTRSGYLTGSQARGVLVQSKLPQVTLAQIWTLSDIDGDGRLNCDEFILAMFLCEKAMAGEKIPVTLPQEWVPPNLRKIKSRPGSVSGVVSRPGSQPASRHASVSSQSGVGVVDADPTAGLPGQTSFEDKRKENYVKGQAELDRRRKIMEDQQRKEREERERKEREEADKREKARLEAERKQQEELERQLQRQREIEMEKEEQRKRELEAKEAARKELEKQRQQEWEQARIAEMNAQKEREQERVLKQKAHNTQLNVELSTLNEKIKELSQRICDTRAGVTNVKTVIDGMRTQRD 497 HSP 3 Score: 105.531 bits (262), Expect = 3.251e-22 Identity = 48/90 (53.33%), Postives = 60/90 (66.67%), Query Frame = 3
Query: 63 NLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLPLSL 332
+ W +T E++K+ QF+ L P GF+TG QAK F +S LP +LG+IW LAD D DG MN NEF+IA LI KL G +VP VLP SL
Sbjct: 6 DAWAVTPRERLKYQEQFRALQPQAGFVTGAQAKGFFLQSQLPPLILGQIWALADTDSDGKMNINEFSIACKLINLKLRGMDVPKVLPPSL 95 HSP 4 Score: 77.411 bits (189), Expect = 1.207e-13 Identity = 59/215 (27.44%), Postives = 95/215 (44.19%), Query Frame = 3
Query: 2052 LVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSECQWAEAIYHW---KPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKY 2687
V Y+A+Y F R +EITF GDII++ + EP GWL G ++ H G FPE YV KL + V+ +++ AD +N++I S++ A + ++ P + G+ LSF G+++ +I +WWTG + G FP YV+ D GT + A + + ++LN + Y
Sbjct: 676 FVKYQAVYEFNARNAEEITFVPGDIILVPLEQNAEP--GWLAGEING--HTGWFPESYVEKLEVGEVAPVAAVEAPVDAQVAT-VADTYNDNINTSSIPAASADLTAAGDVEYYIAAYPYESAEEGD----LSFSAGEMVMVIKKE-GEWWTGTIGSR---------TGMFPSNYVQ--------KADVGTASTAAAEPVESLDQETTLNGNAAY 863 HSP 5 Score: 68.9366 bits (167), Expect = 4.346e-11 Identity = 31/90 (34.44%), Postives = 53/90 (58.89%), Query Frame = 3
Query: 57 NPNLWFITSEEKVKFDVQFQCLNPV-NGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLP 323
N W + + +K K+ F + +G++TG+QA+ + +S LP L +IW L+D D DG +N +EF +AM L ++ + G ++P LP
Sbjct: 181 NQGEWAVQAAQKRKYTQVFNANDRTRSGYLTGSQARGVLVQSKLPQVTLAQIWTLSDIDGDGRLNCDEFILAMFLCEKAMAGEKIPVTLP 270 HSP 6 Score: 65.0846 bits (157), Expect = 6.723e-10 Identity = 33/77 (42.86%), Postives = 44/77 (57.14%), Query Frame = 3
Query: 651 DDWVIPQSVRPRYRLQFNQQDRN---KRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLV 872
D W +V PR RL++ +Q R + GF+TG +AK +QS LP L IW+LAD D DG + EF IA L+
Sbjct: 6 DAW----AVTPRERLKYQEQFRALQPQAGFVTGAQAKGFFLQSQLPPLILGQIWALADTDSDGKMNINEFSIACKLI 78
BLAST of Intersectin-1 vs. Ensembl Fly
Match: Dap160 (gene:FBgn0023388 transcript:FBtr0306017) HSP 1 Score: 143.665 bits (361), Expect = 6.140e-34 Identity = 125/442 (28.28%), Postives = 204/442 (46.15%), Query Frame = 3
Query: 2433 QLSFETGQLIKI-IDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKY-----------SSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSA-----------ISLNSVTSNPYST---------------ISDVENNSALYYPQN----TVPVNSNGASTP-------EIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYV 3611
+++F G +I + ++ N + W A + NG GWFP++YV L E P + P + +A Y +S +L+ D+ + +A YPY+++ GDL+ G+++ V+ ++ +WW G + G+FPSNYV+ A+ +A A PVE + + NA+ +A + + + P S+ +S + S + + P++ + TP EIAQV+ + S EQ+ L GQLI IR+K+ SGWW+GE+Q +G++R+IGWFP YV ++ + N G ++T S IE+ + + D +I +Y Y AQN DELSFDK+ I+ ++ ++EPEWW G L+ GLFP NYV
Sbjct: 688 EITFVPGDIILVPLEQNAEPGWL----AGEINGH----TGWFPESYVEKL-EVGEVAPVAAVEAPVDAQVATVADTYNDNINTSSIPAASADLTAAGDVEYY--------IAAYPYESAEEGDLSFSAGEMVMVIKKEGEWWTGTIGSRT--GMFPSNYVQ------KADVGTASTAAAEPVESLDQETTLNGNAAYTAAPVEAQEQVYQPLPVQEPSEQPISSPGVGAEEAHEDLDTEVSQINTQSKTQSSEPAESYSRPMSRTSSMTPGMRAKRSEIAQVIAPYEATSTEQLSLTRGQLIMIRKKTDSGWWEGELQAKGRRRQIGWFPATYVKVLQGGR--------NSG------------------RNTPVSGSRIEMTEQIL-DKVIALYPYKAQNDDELSFDKDDIISVLGRDEPEWWRGE--LNGLSGLFPSNYV 1075 HSP 2 Score: 111.694 bits (278), Expect = 4.375e-24 Identity = 119/320 (37.19%), Postives = 191/320 (59.69%), Query Frame = 3
Query: 624 PPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXKKNILGTSMSASMNIPIN-PITNGVSVTXXXXXXXXXXXXXV-LTGEDERRANYLKGQAELEKRRAILDEQMRQXXXXXXXXXXXXXXXXXXXXQEKEQARFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKMRLESEKKRLLEMEKRRLEVAIXXXXXXXXXXXXVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYKAGIGSMREHRD 1577
P I +V + +W + + + +Y FN DR + G+LTG +A+ +LVQS LP +LA IW+L+DID DG L C+EF +AM L E+A +G ++P LPQ +PP+L+K S+S ++ P + P + SV+ + L G+++R+ NY+KGQAEL++RR I+++Q R+E E R+ KER E ++ EK R E E+ + E++RQ++ +R+ E+E+EEQ +R E +E R E EK+R E E+ R+ +++ +EQE + + + L L+ EK ++L RI T+ + K I MR RD
Sbjct: 175 PSIESV-NQGEWAVQAAQKRKYTQVFNANDRTRSGYLTGSQARGVLVQSKLPQVTLAQIWTLSDIDGDGRLNCDEFILAMFLCEKAMAGEKIPVTLPQEWVPPNLRKIKSRPGSVSGVVSRPGSQPASRHASVSSQSGVGVVDADPTAGLPGQNKRKENYVKGQAELDRRRKIMEDQQRKEREERERKEREEADKREKARLEAERKQQEELERQLQRQREIEMEKEEQRKRELEAKEAARKELEKQRQQEWEQARIAEMNAQKEREQERVLKQKAHNTQLNVELSTLNEKIKELSQRICDTRAGVTNVKTVIDGMRTQRD 493 HSP 3 Score: 105.145 bits (261), Expect = 4.810e-22 Identity = 48/90 (53.33%), Postives = 60/90 (66.67%), Query Frame = 3
Query: 63 NLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLPLSL 332
+ W +T E++K+ QF+ L P GF+TG QAK F +S LP +LG+IW LAD D DG MN NEF+IA LI KL G +VP VLP SL
Sbjct: 6 DAWAVTPRERLKYQEQFRALQPQAGFVTGAQAKGFFLQSQLPPLILGQIWALADTDSDGKMNINEFSIACKLINLKLRGMDVPKVLPPSL 95 HSP 4 Score: 77.0258 bits (188), Expect = 1.640e-13 Identity = 59/215 (27.44%), Postives = 95/215 (44.19%), Query Frame = 3
Query: 2052 LVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSECQWAEAIYHW---KPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKY 2687
V Y+A+Y F R +EITF GDII++ + EP GWL G ++ H G FPE YV KL + V+ +++ AD +N++I S++ A + ++ P + G+ LSF G+++ +I +WWTG + G FP YV+ D GT + A + + ++LN + Y
Sbjct: 672 FVKYQAVYEFNARNAEEITFVPGDIILVPLEQNAEP--GWLAGEING--HTGWFPESYVEKLEVGEVAPVAAVEAPVDAQVAT-VADTYNDNINTSSIPAASADLTAAGDVEYYIAAYPYESAEEGD----LSFSAGEMVMVIKKE-GEWWTGTIGSR---------TGMFPSNYVQ--------KADVGTASTAAAEPVESLDQETTLNGNAAY 859 HSP 5 Score: 75.485 bits (184), Expect = 5.177e-13 Identity = 57/158 (36.08%), Postives = 78/158 (49.37%), Query Frame = 3
Query: 2433 QLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLL--CVARYPYQASVLGDLNIQPGDIIEVLDQKQ-DWWKGYNRNTNSQGLFPSNYVK-IVTSE 2894
QLS GQLI I WW GE L A G+ + I GWFP TYV++L +SG TP +GS ++ ++++L +A YPY+A +L+ DII VL + + +WW+G + GLFPSNYV VTSE
Sbjct: 955 QLSLTRGQLIMIRKKTDSGWWEGE---LQAKGRRRQI-GWFPATYVKVLQ----GGRNSGRNTPVSGSRIEM--------------------TEQILDKVIALYPYKAQNDDELSFDKDDIISVLGRDEPEWWRGELNGLS--GLFPSNYVGPFVTSE 1082 HSP 6 Score: 68.9366 bits (167), Expect = 4.792e-11 Identity = 32/95 (33.68%), Postives = 54/95 (56.84%), Query Frame = 3
Query: 57 NPNLWFITSEEKVKFDVQFQCLNPV-NGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLPLSLNP 338
N W + + +K K+ F + +G++TG+QA+ + +S LP L +IW L+D D DG +N +EF +AM L ++ + G ++P LP P
Sbjct: 181 NQGEWAVQAAQKRKYTQVFNANDRTRSGYLTGSQARGVLVQSKLPQVTLAQIWTLSDIDGDGRLNCDEFILAMFLCEKAMAGEKIPVTLPQEWVP 275 HSP 7 Score: 64.6994 bits (156), Expect = 1.037e-9 Identity = 33/77 (42.86%), Postives = 44/77 (57.14%), Query Frame = 3
Query: 651 DDWVIPQSVRPRYRLQFNQQDRN---KRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLV 872
D W +V PR RL++ +Q R + GF+TG +AK +QS LP L IW+LAD D DG + EF IA L+
Sbjct: 6 DAW----AVTPRERLKYQEQFRALQPQAGFVTGAQAKGFFLQSQLPPLILGQIWALADTDSDGKMNINEFSIACKLI 78
BLAST of Intersectin-1 vs. Ensembl Fly
Match: Dap160 (gene:FBgn0023388 transcript:FBtr0333087) HSP 1 Score: 140.198 bits (352), Expect = 5.867e-33 Identity = 116/405 (28.64%), Postives = 183/405 (45.19%), Query Frame = 3
Query: 2433 QLSFETGQLIKI-IDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKY-----------SSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALYYPQNTVPVNSNGASTPEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYV 3611
+++F G +I + ++ N + W A + NG GWFP++YV L E P + P + +A Y +S +L+ D+ + +A YPY+++ GDL+ G+++ V+ ++ +WW G + G+FPSNYV+ A+ +A A PVE + + + A EIAQV+ + S EQ+ L GQLI IR+K+ SGWW+GE+Q +G++R+IGWFP YV ++ + N G ++T S IE+ + + D +I +Y Y AQN DELSFDK+ I+ ++ ++EPEWW G L+ GLFP NYV
Sbjct: 692 EITFVPGDIILVPLEQNAEPGWL----AGEINGH----TGWFPESYVEKL-EVGEVAPVAAVEAPVDAQVATVADTYNDNINTSSIPAASADLTAAGDVEYY--------IAAYPYESAEEGDLSFSAGEMVMVIKKEGEWWTGTIGSRT--GMFPSNYVQ------KADVGTASTAAAEPVESLDQGM----------------------------------------------RAKRSEIAQVIAPYEATSTEQLSLTRGQLIMIRKKTDSGWWEGELQAKGRRRQIGWFPATYVKVLQGGR--------NSG------------------RNTPVSGSRIEMTEQIL-DKVIALYPYKAQNDDELSFDKDDIISVLGRDEPEWWRGE--LNGLSGLFPSNYV 996 HSP 2 Score: 109.383 bits (272), Expect = 2.155e-23 Identity = 121/324 (37.35%), Postives = 191/324 (58.95%), Query Frame = 3
Query: 624 PPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXKKNILGTSMSASMNIPIN-PITNGVSVTXXXXXXXXXXXXXV-LTG----EDERRANYLKGQAELEKRRAILDEQMRQXXXXXXXXXXXXXXXXXXXXQEKEQARFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKMRLESEKKRLLEMEKRRLEVAIXXXXXXXXXXXXVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYKAGIGSMREHRD 1577
P I +V + +W + + + +Y FN DR + G+LTG +A+ +LVQS LP +LA IW+L+DID DG L C+EF +AM L E+A +G ++P LPQ +PP+L+K S+S ++ P + P + SV+ + L G ED+R+ NY+KGQAEL++RR I+++Q R+E E R+ KER E ++ EK R E E+ + E++RQ++ +R+ E+E+EEQ +R E +E R E EK+R E E+ R+ +++ +EQE + + + L L+ EK ++L RI T+ + K I MR RD
Sbjct: 175 PSIESV-NQGEWAVQAAQKRKYTQVFNANDRTRSGYLTGSQARGVLVQSKLPQVTLAQIWTLSDIDGDGRLNCDEFILAMFLCEKAMAGEKIPVTLPQEWVPPNLRKIKSRPGSVSGVVSRPGSQPASRHASVSSQSGVGVVDADPTAGLPGQTSFEDKRKENYVKGQAELDRRRKIMEDQQRKEREERERKEREEADKREKARLEAERKQQEELERQLQRQREIEMEKEEQRKRELEAKEAARKELEKQRQQEWEQARIAEMNAQKEREQERVLKQKAHNTQLNVELSTLNEKIKELSQRICDTRAGVTNVKTVIDGMRTQRD 497 HSP 3 Score: 104.76 bits (260), Expect = 4.360e-22 Identity = 48/90 (53.33%), Postives = 60/90 (66.67%), Query Frame = 3
Query: 63 NLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLPLSL 332
+ W +T E++K+ QF+ L P GF+TG QAK F +S LP +LG+IW LAD D DG MN NEF+IA LI KL G +VP VLP SL
Sbjct: 6 DAWAVTPRERLKYQEQFRALQPQAGFVTGAQAKGFFLQSQLPPLILGQIWALADTDSDGKMNINEFSIACKLINLKLRGMDVPKVLPPSL 95 HSP 4 Score: 94.7449 bits (234), Expect = 6.398e-19 Identity = 90/323 (27.86%), Postives = 142/323 (43.96%), Query Frame = 3
Query: 1926 AAIAILKKQKEPKTAVSEKNYNEFDKIGISKNPQLNS--MPSNELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLS-GPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSECQWAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLL--CVARYPYQASVLGDLNIQPGDIIEVLDQKQ-DWWKGYNRNTNSQGLFPSNYV 2876
A +A ++ + + A YN D I S P ++ + ++ YY A Y +E + +++F G+++++ I+ W G + S G+FP YV+K G ST + + ++Q G K SE A+ I ++ S QLS GQLI I WW GE L A G+ + I GWFP TYV++L +SG TP +GS ++ ++++L +A YPY+A +L+ DII VL + + +WW+G + GLFPSNYV
Sbjct: 739 APVAAVEAPVDAQVATVADTYN--DNINTSSIPAASADLTAAGDVEYYIAAYPYESAEEGDLSFSAGEMVMV-----IKKEGEWWTGTI--GSRTGMFPSNYVQKADVGTASTAAAEPVESLDQ-----------------GMRAKRSEI--AQVIAPYEATST-------EQLSLTRGQLIMIRKKTDSGWWEGE---LQAKGRRRQI-GWFPATYVKVLQ----GGRNSGRNTPVSGSRIEM--------------------TEQILDKVIALYPYKAQNDDELSFDKDDIISVLGRDEPEWWRGELNGLS--GLFPSNYV 996 HSP 5 Score: 89.3521 bits (220), Expect = 2.786e-17 Identity = 76/286 (26.57%), Postives = 126/286 (44.06%), Query Frame = 3
Query: 2052 LVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSECQWAEAIYHW---KPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQD--WWKGYNRNTNSQ---GLFPSNYVKIV 2885
V Y+A+Y F R +EITF GDII++ + EP GWL G ++ H G FPE YV KL + V+ +++ AD +N++I S++ A + ++ P + G+ LSF G+++ +I +WWTG + G FP YV+ D GT + A A SL+ ++ + + PY+A+ L++ G +I ++ +K D WW+G + + G FP+ YVK++
Sbjct: 676 FVKYQAVYEFNARNAEEITFVPGDIILVPLEQNAEP--GWLAGEING--HTGWFPESYVEKLEVGEVAPVAAVEAPVDAQVAT-VADTYNDNINTSSIPAASADLTAAGDVEYYIAAYPYESAEEGD----LSFSAGEMVMVIKKE-GEWWTGTIGSR---------TGMFPSNYVQ--------KADVGTASTAA------AEPVESLDQGMR-------AKRSEIAQVIAPYEATSTEQLSLTRGQLI-MIRKKTDSGWWEGELQAKGRRRQIGWFPATYVKVL 920 HSP 6 Score: 68.9366 bits (167), Expect = 4.708e-11 Identity = 32/95 (33.68%), Postives = 54/95 (56.84%), Query Frame = 3
Query: 57 NPNLWFITSEEKVKFDVQFQCLNPV-NGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLPLSLNP 338
N W + + +K K+ F + +G++TG+QA+ + +S LP L +IW L+D D DG +N +EF +AM L ++ + G ++P LP P
Sbjct: 181 NQGEWAVQAAQKRKYTQVFNANDRTRSGYLTGSQARGVLVQSKLPQVTLAQIWTLSDIDGDGRLNCDEFILAMFLCEKAMAGEKIPVTLPQEWVP 275 HSP 7 Score: 64.6994 bits (156), Expect = 8.220e-10 Identity = 33/77 (42.86%), Postives = 44/77 (57.14%), Query Frame = 3
Query: 651 DDWVIPQSVRPRYRLQFNQQDRN---KRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLV 872
D W +V PR RL++ +Q R + GF+TG +AK +QS LP L IW+LAD D DG + EF IA L+
Sbjct: 6 DAW----AVTPRERLKYQEQFRALQPQAGFVTGAQAKGFFLQSQLPPLILGQIWALADTDSDGKMNINEFSIACKLI 78
BLAST of Intersectin-1 vs. Ensembl Zebrafish
Match: itsn1 (intersectin 1 (SH3 domain protein) [Source:ZFIN;Acc:ZDB-GENE-030616-226]) HSP 1 Score: 564.688 bits (1454), Expect = 4.971e-170 Identity = 357/973 (36.69%), Postives = 518/973 (53.24%), Query Frame = 3
Query: 2373 AEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLAS-KYSSLNLSVKYDL--------NTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALYYPQNTVPVNSNGASTPEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGD----HLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYNTG--GMPFVFPLETSSKDKMQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMF 5240
A+A+Y W+ + + N L+F +I +++ D WW GE GWFPK+YV+L+ + P +++ +GS + S K S +L+ +L N+ S++ VA Y Y+++ GDL Q GD+I V ++ DWW G + G+FPSNYVK SE L SA S+ P I+ V P + GA EQ+ L GQLI IR+K+P GWW+GE+Q RG++R+IGWFP YV + ++ TS T LP +I MY+Y+AQN DEL F K I+ ++ + +P+WW G L+ GLFP NYV+ + S + W D + +LT E++RQ YI ELI TE+ Y DLQ V + F +P+ +S+LLT E+ IF+NW +L + AL++RK ++G + P++ +GDIL + P+ Y+RFCS Q G +Q+K P+FK+ + + RC+ MP++S+ LKPMQR+T+Y L+++NIL++T ++HPD S + A+ A LC VNEGVRE+ENS+RLEW+Q H+QCE LSE+L FNS TNCLG RK +HSG L K KS KEL GFLFNDF LL G G VF S+K +Q++MYK PI LN+V++ PT + + IF + + +RA S N+R WV+++ AS +I TE KRE + +R R G+GRL+V +VEGI L+ + KS+P+CE ++G+Q H TK + TLNPKWN+ QF IK+++QDVLC+TV+ +D F+P+DFLGR E+ + ++ ++ ++ PI KRL L EV GE+VVRLDL +F
Sbjct: 904 AQALYPWRAKKD-------NHLNFNKNDVITVLEQQ-DMWWFGEVQGQ---------RGWFPKSYVKLI-----SGPVRKSMSIESGSSDSPPSVKRPSPSLNKPTELGEGQNSNSNSMYPSKEY--VAMYTYESNEQGDLTFQQGDVITVTKKEGDWWTGT--VSGKIGVFPSNYVKPKESEGLG-----------------------------SAGKTGSLGKKP--EIAQV-----------IAPYTATGA-----------------EQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKL--------------------------------LSPSTSKTTPTDPNPPKLPTPNAVCQVIGMYDYTAQNDDELPFGKGQIINVLSREDPDWWKGE--LNGSVGLFPSNYVKLTTDTDPSQQ-----------------------WCAD--LHLLDMLTPVERKRQGYIHELIVTEENYVNDLQLVTETFQKPLLESDLLTEKEVAMIFVNWKELTMCNIKLLKALRVRKKMSGERMPVKMIGDILTAQLPHMQPYIRFCSCQLNGATLIQQKTDEVPEFKDFVKRLAMDPRCKGMPLSSFLLKPMQRVTRYPLIIKNILENTPESHPDHSHLRLALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGTDKVF----SAKSHLQYRMYKTPIFLNEVLVK--LPTDPSGDE----PIFHISHIDRVYTIRAESINERTAWVQKIKAASELFIETEKRKREKAYLVRSQRATGIGRLMVNIVEGIELK--PCRSNGKSNPYCEVTMGSQCHVTKTLQDTLNPKWNSNCQFFIKDLEQDVLCVTVFERDQFSPDDFLGRTEIRLADIKKDQG-SKGPITKRLLLHEVPTGEIVVRLDLQLF 1719 HSP 2 Score: 170.244 bits (430), Expect = 9.199e-42 Identity = 135/382 (35.34%), Postives = 196/382 (51.31%), Query Frame = 3
Query: 63 NLWFITSEEKVKFDVQFQCLNPV-NGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGY---------------EVPDVLPLSLNPLQTNATTMSYSGYMPVSNTYNTMPVMHNTSHAMSNNNPNNTMAVPHASAFVNYXXXXXXXXXXXXXXXXNAQFGSXXXXXXXXXXSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXK-KNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAILDEQMRQ 1157
+ W I+ +E+ K D QF L P GFITG+QA++ F +S LP +L +IW LAD + DG M+ +EF+IAM LIK KL+G+ +P P + P + + S PV + N P M S H A +N K+ S + S+ Q G Q + P DW + QS R +YR FN D+ G+LTG +A+ IL+QS LP LA+IW+L+DIDQDG LT EEF +AMHL++ A SG LP LP L+PP+ ++ ++ G S+++ + T+ S + + EKE+++K +T ED++R N+ +G ELEKRR L EQ R+
Sbjct: 13 DTWVISVDERAKHDQQFHSLTPTPAGFITGDQARNFFLQSGLPAPILAQIWALADMNNDGKMDMHEFSIAMKLIKLKLQGHPLPPSLPPSMKQQPISIPPPTPFGV-PGMGVSPPLGASVTPPVPSLANGAPAMIQPLSGFS-----------HPGAVLN-------KTPSFNRSSIKLQKG----------------QSVEAPSAPAAPPPADWAVSQSSRLKYRQLFNSHDKMMSGYLTGPQARTILMQSSLPQAQLATIWNLSDIDQDGKLTAEEFILAMHLIDMAMSGLPLPPLLPPDLIPPTFRRMRSGSGVSVTSMHS------TDLRSQEEPEEEEKEAEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRK 353 HSP 3 Score: 95.9005 bits (237), Expect = 4.012e-19 Identity = 97/329 (29.48%), Postives = 135/329 (41.03%), Query Frame = 3
Query: 1917 KKNAAIAILKKQKEPKTAVSEKNYNEFDKIGISKNPQLNSM-PSNELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSECQWAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQ-DWWKGYNRNTNSQGLFPSNYVKIVTSED 2897
+K+ +I P + N+ ++G +N NSM PS E Y A+Y +E + ++TF+ GD+I + ++ W G + S G+FP YV+ P +E S+G G+L K E A Y T E QL+ GQLI I N WW GE L A GK++ I GWFP YV+LL P + TPT + L + + + + Y Y A +L G II VL ++ DWWKG S GLFPSNYVK+ T D
Sbjct: 960 RKSMSIESGSSDSPPSVKRPSPSLNKPTELGEGQNSNSNSMYPSKE---YVAMYTYESNEQGDLTFQQGDVITVTKKE-----GDWWTGTV--SGKIGVFPSNYVK----PKESEGLG---------SAGKT----------GSLGKKPEIAQVIAPY------TATGAE---QLTLAPGQLILIRKKNPGGWWEGE---LQARGKKRQI-GWFPANYVKLLS------PSTSKTTPTDPNPPKLPTPNAVCQV-----------------IGMYDYTAQNDDELPFGKGQIINVLSREDPDWWKG--ELNGSVGLFPSNYVKLTTDTD 1217 HSP 4 Score: 63.929 bits (154), Expect = 2.590e-9 Identity = 76/306 (24.84%), Postives = 125/306 (40.85%), Query Frame = 3
Query: 2007 GISKNPQLNSMPSNELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSECQWAE-----AIYHWKPESNVTYGEYPNQ---LSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQ-DWWKG--YNRNTNSQ-GLFPSNYVKIVT 2888
G S +P L E + +A+Y + ++ + + F D+I + Q + W +G + G FP+ YV+ +SGP + + +I SG +D + P +L K +E + ++Y K E Y N+ L+F+ G +I + DWWTG + GK +G FP YV+ + + GS A K SL G + + PY A+ L + PG +I + + WW+G R Q G FP+NYVK+++
Sbjct: 886 GSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDM-----WWFG--EVQGQRGWFPKSYVKLISGP---------VRKSMSIESGSSDSPPSVKRPSPSLNKPTELGEGQNSNSNSMYPSK-EYVAMYTYESNEQGDLTFQQGDVITVTKKE-GDWWTGTVS-----GK----IGVFPSNYVKPKESEG------------LGS----AGKTGSL------------GKKPEIAQVIAPYTATGAEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLS 1136 HSP 5 Score: 57.3806 bits (137), Expect = 2.304e-7 Identity = 27/63 (42.86%), Postives = 37/63 (58.73%), Query Frame = 3
Query: 2049 ELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKL 2237
++VYY A+Y FE R HDEIT GDI++++ EP GWL G + + G FP Y K+
Sbjct: 704 KVVYYRAMYPFEARSHDEITIHPGDIVMVDESQTGEP--GWLGGEIKGKT--GWFPANYAEKI 762
BLAST of Intersectin-1 vs. Ensembl Zebrafish
Match: itsn1 (intersectin 1 (SH3 domain protein) [Source:ZFIN;Acc:ZDB-GENE-030616-226]) HSP 1 Score: 563.148 bits (1450), Expect = 1.600e-169 Identity = 356/973 (36.59%), Postives = 516/973 (53.03%), Query Frame = 3
Query: 2373 AEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLAS-KYSSLNLSVKYDL--------NTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALYYPQNTVPVNSNGASTPEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGD----HLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYNTG--GMPFVFPLETSSKDKMQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMF 5240
A+A+Y W+ + + N L+F +I +++ D WW GE GWFPK+YV+L+ + P +++ +GS + S K S +L+ +L N+ S++ VA Y Y+++ GDL Q GD+I V ++ DWW G + G+FPSNYVK SE L SA S+ P Q P + GA EQ+ L GQLI IR+K+P GWW+GE+Q RG++R+IGWFP YV + ++ TS T LP +I MY+Y+AQN DEL F K I+ ++ + +P+WW G L+ GLFP NYV+ + S + W D + +LT E++RQ YI ELI TE+ Y DLQ V + F +P+ +S+LLT E+ IF+NW +L + AL++RK ++G + P++ +GDIL + P+ Y+RFCS Q G +Q+K P+FK+ + + RC+ MP++S+ LKPMQR+T+Y L+++NIL++T ++HPD S + A+ A LC VNEGVRE+ENS+RLEW+Q H+QCE LSE+L FNS TNCLG RK +HSG L K KS KEL GFLFNDF LL G G VF S+K +Q++MYK PI LN+V++ PT + + IF + + +RA S N+R WV+++ AS +I TE KRE + +R R G+GRL+V +VEGI L+ + KS+P+CE ++G+Q H TK + TLNPKWN+ QF IK+++QDVLC+TV+ +D F+P+DFLGR E+ + ++ ++ ++ PI KRL L EV GE+VVRLDL +F
Sbjct: 903 AQALYPWRAKKD-------NHLNFNKNDVITVLEQQ-DMWWFGEVQGQ---------RGWFPKSYVKLI-----SGPVRKSMSIESGSSDSPPSVKRPSPSLNKPTELGEGQNSNSNSMYPSKEY--VAMYTYESNEQGDLTFQQGDVITVTKKEGDWWTGT--VSGKIGVFPSNYVKPKESEGLG-----------------------------SAGKTGSLGKKP-------------EIAQVIAPYTATGA-----------------EQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKL--------------------------------LSPSTSKTTPTDPNPPKLPTPNAVCQVIGMYDYTAQNDDELPFGKGQIINVLSREDPDWWKGE--LNGSVGLFPSNYVKLTTDTDPSQQ-----------------------WCAD--LHLLDMLTPVERKRQGYIHELIVTEENYVNDLQLVTETFQKPLLESDLLTEKEVAMIFVNWKELTMCNIKLLKALRVRKKMSGERMPVKMIGDILTAQLPHMQPYIRFCSCQLNGATLIQQKTDEVPEFKDFVKRLAMDPRCKGMPLSSFLLKPMQRVTRYPLIIKNILENTPESHPDHSHLRLALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGTDKVF----SAKSHLQYRMYKTPIFLNEVLVK--LPTDPSGDE----PIFHISHIDRVYTIRAESINERTAWVQKIKAASELFIETEKRKREKAYLVRSQRATGIGRLMVNIVEGIELK--PCRSNGKSNPYCEVTMGSQCHVTKTLQDTLNPKWNSNCQFFIKDLEQDVLCVTVFERDQFSPDDFLGRTEIRLADIKKDQG-SKGPITKRLLLHEVPTGEIVVRLDLQLF 1718 HSP 2 Score: 169.859 bits (429), Expect = 1.379e-41 Identity = 135/382 (35.34%), Postives = 196/382 (51.31%), Query Frame = 3
Query: 63 NLWFITSEEKVKFDVQFQCLNPV-NGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGY---------------EVPDVLPLSLNPLQTNATTMSYSGYMPVSNTYNTMPVMHNTSHAMSNNNPNNTMAVPHASAFVNYXXXXXXXXXXXXXXXXNAQFGSXXXXXXXXXXSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXK-KNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAILDEQMRQ 1157
+ W I+ +E+ K D QF L P GFITG+QA++ F +S LP +L +IW LAD + DG M+ +EF+IAM LIK KL+G+ +P P + P + + S PV + N P M S H A +N K+ S + S+ Q G Q + P DW + QS R +YR FN D+ G+LTG +A+ IL+QS LP LA+IW+L+DIDQDG LT EEF +AMHL++ A SG LP LP L+PP+ ++ ++ G S+++ + T+ S + + EKE+++K +T ED++R N+ +G ELEKRR L EQ R+
Sbjct: 13 DTWVISVDERAKHDQQFHSLTPTPAGFITGDQARNFFLQSGLPAPILAQIWALADMNNDGKMDMHEFSIAMKLIKLKLQGHPLPPSLPPSMKQQPISIPPPTPFGV-PGMGVSPPLGASVTPPVPSLANGAPAMIQPLSGFS-----------HPGAVLN-------KTPSFNRSSIKLQKG----------------QSVEAPSAPAAPPPADWAVSQSSRLKYRQLFNSHDKMMSGYLTGPQARTILMQSSLPQAQLATIWNLSDIDQDGKLTAEEFILAMHLIDMAMSGLPLPPLLPPDLIPPTFRRMRSGSGVSVTSMHS------TDLRSQEEPEEEEKEAEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRK 353 HSP 3 Score: 95.9005 bits (237), Expect = 4.327e-19 Identity = 97/329 (29.48%), Postives = 135/329 (41.03%), Query Frame = 3
Query: 1917 KKNAAIAILKKQKEPKTAVSEKNYNEFDKIGISKNPQLNSM-PSNELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSECQWAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQ-DWWKGYNRNTNSQGLFPSNYVKIVTSED 2897
+K+ +I P + N+ ++G +N NSM PS E Y A+Y +E + ++TF+ GD+I + ++ W G + S G+FP YV+ P +E S+G G+L K E A Y T E QL+ GQLI I N WW GE L A GK++ I GWFP YV+LL P + TPT + L + + + + Y Y A +L G II VL ++ DWWKG S GLFPSNYVK+ T D
Sbjct: 959 RKSMSIESGSSDSPPSVKRPSPSLNKPTELGEGQNSNSNSMYPSKE---YVAMYTYESNEQGDLTFQQGDVITVTKKE-----GDWWTGTV--SGKIGVFPSNYVK----PKESEGLG---------SAGKT----------GSLGKKPEIAQVIAPY------TATGAE---QLTLAPGQLILIRKKNPGGWWEGE---LQARGKKRQI-GWFPANYVKLLS------PSTSKTTPTDPNPPKLPTPNAVCQV-----------------IGMYDYTAQNDDELPFGKGQIINVLSREDPDWWKG--ELNGSVGLFPSNYVKLTTDTD 1216 HSP 4 Score: 63.5438 bits (153), Expect = 3.117e-9 Identity = 76/306 (24.84%), Postives = 125/306 (40.85%), Query Frame = 3
Query: 2007 GISKNPQLNSMPSNELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSECQWAE-----AIYHWKPESNVTYGEYPNQ---LSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQ-DWWKG--YNRNTNSQ-GLFPSNYVKIVT 2888
G S +P L E + +A+Y + ++ + + F D+I + Q + W +G + G FP+ YV+ +SGP + + +I SG +D + P +L K +E + ++Y K E Y N+ L+F+ G +I + DWWTG + GK +G FP YV+ + + GS A K SL G + + PY A+ L + PG +I + + WW+G R Q G FP+NYVK+++
Sbjct: 885 GSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDM-----WWFG--EVQGQRGWFPKSYVKLISGP---------VRKSMSIESGSSDSPPSVKRPSPSLNKPTELGEGQNSNSNSMYPSK-EYVAMYTYESNEQGDLTFQQGDVITVTKKE-GDWWTGTVS-----GK----IGVFPSNYVKPKESEG------------LGS----AGKTGSL------------GKKPEIAQVIAPYTATGAEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLS 1135 HSP 5 Score: 57.3806 bits (137), Expect = 2.527e-7 Identity = 27/63 (42.86%), Postives = 37/63 (58.73%), Query Frame = 3
Query: 2049 ELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKL 2237
++VYY A+Y FE R HDEIT GDI++++ EP GWL G + + G FP Y K+
Sbjct: 703 KVVYYRAMYPFEARSHDEITIHPGDIVMVDESQTGEP--GWLGGEIKGKT--GWFPANYAEKI 761
BLAST of Intersectin-1 vs. Ensembl Zebrafish
Match: itsn2a (intersectin 2a [Source:ZFIN;Acc:ZDB-GENE-041014-84]) HSP 1 Score: 499.59 bits (1285), Expect = 1.510e-147 Identity = 330/928 (35.56%), Postives = 482/928 (51.94%), Query Frame = 3
Query: 2547 GWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSES-IIDLMNAS-----------QHSAISLNSVTSNPYSTISDVENNSALYYPQNTV-PVNSNGAST------PEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYNTGGMPFVFPLETSSKDKM-------QFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMFKK 5246
GWFP++Y + + P + TA S + +Q VA + A L DII VL+++++WW Y +G FP ++V +VT+ NT+S V E+S+S ++D A SA + VT + +P N V P + AS+ PEIAQV + EQ+ L GQLI + K+ + WW GE+Q RG++R+ GWF V ++ + N G +TP P +I MY+Y A N DE+SF K ++ +++K+ P+WW G ++ GLFP NYV+ + S + W D ++ +++ TE E++RQ YI ELI+TE+ Y EDL+ +VF++P+ +S LT E+ IF+NW +LI S AL++RK AG + P+Q VGDIL S + Y+RFCS Q A LQ+K FK + SN RC+ MP++S+ LKPMQRIT+Y LL++NIL++T H D S+++ A+ A LC VNE VRE+ENS+RLEW+Q H+ C+ + E L FNS TNCLG RKL+HSG L K KS KEL LFNDF LL Y + +SS DK+ Q+KMYK P+ LN+V++ S SS + F + I V + L+ + N+R WV+++ AS +I TE LKRE + R + +G+GRLLVTV+E L+ + KS+P+CE ++G Q + ++ P TLNPKW+ QF IK++ QDVLCLTV+ +D F+P+DFLGR EV V + ++ + P+ +RL L EV GE+ VRLDL ++++
Sbjct: 796 GWFPESYAERCSKDSQTQPTAAETESTAPSANYPGIPRVDIEGPTPTHTPVSTNTQHSQAVALCEWSAKTDSHLGFCKDDIITVLEKQENWW--YGELKEKRGWFPCSHVSVVTTN---NTQSEPLYSSVEEIEVSDSGLLDEYVALYTYESPESGDLTFSAEDVVLVTEKEGEWWRGCIGDQTGLFPSNYVKPKEPDTASSAVPSKKPEIAQVTTVSPATTPEQLSLTPGQLIVVLHKNSNSWWLGELQARGKKRKKGWFQSSNVRLLEA----------NSGKITPAPQ---------------------------PLCQVIAMYDYKAANKDEMSFQKGQLITVLNKDNPDWWKGE--VAGVTGLFPTNYVKMTTECDPSQQ-----------------------WCAD-LLSLDSMCTE-ERKRQGYIHELIQTEESYLEDLELAVEVFYKPMAESGRLTEAEISMIFVNWRELIMCSTKLLKALRVRKKTAGERMPVQVVGDILSSELSHMQAYIRFCSCQLNAAALLQQKTDKSLDFKLFLKKIASNYRCKGMPLSSFLLKPMQRITRYPLLIKNILENTPPAHADHSNLQAALEQAEELCSQVNEAVREKENSDRLEWIQNHVLCDGVIEHLVFNSLTNCLGPRKLLHSGKLHKTKSSKELWALLFNDFLLLT---YTSKQF-------SSSPDKLFNPNSNTQYKMYKTPVFLNEVLVKMPSDPSSDDPVFHISHIDRV------YTLKTDTINERTTWVQKIKAASDHFIETEKLKREKAYQARSQKNSGIGRLLVTVLEATELK--PCKPNGKSNPYCELTMGAQCYTSRHQPDTLNPKWSFNCQFFIKDLYQDVLCLTVFERDQFSPDDFLGRTEVPVATIKKDQD-GKGPLVRRLLLHEVPTGEVKVRLDLQLYEQ 1635 HSP 2 Score: 167.548 bits (423), Expect = 6.612e-41 Identity = 131/388 (33.76%), Postives = 191/388 (49.23%), Query Frame = 3
Query: 63 NLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLPLSL----NPLQTNAT--------TMSYSGYMPVSNTYNT-----------------MPVMHNTSHAMSNNNPNNTMAVPHASAFVNYXXXXXXXXXXXXXXXXNAQFGSXXXXXXXXXXSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXKKNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAIL 1139
++W IT EE+ K D QF L P G+++G+QA+ F +S LPT VL IW LAD KDG M+ EF+IAM LIK +L+G +P LP+ + P+ T+++ +S MP+ T +P M N+ + N N T+ P + V+ S T + S++ + + P+ TS DW +PQS R +YR FN D+ G+L+G + + L S L T LA+IW LAD+D+DG L EEF +AMHLVE A++GR LPP L ++ T +N P+ I+ T+ I+ E + KS ++ ED+ R N+ +G AELEKRR L
Sbjct: 6 SIWAITPEERGKHDKQFDSLAPTLGYLSGDQARKFFLQSGLPTSVLADIWALADIGKDGKMDRLEFSIAMKLIKLQLQGQPLPSSLPIIMKQTPTPVMTSSSRFGMGSMPNLSAMSVMPILTPIPTCPVMAPLVPVPTLISGPLPQMPNSISVPALPNGNATLLTPTPAFPVS--------SGLTKSHSLLDLGSSSSNSSSTTSLASNSPK----------TSSSDWAVPQSSRLKYRQLFNSLDKLMSGYLSGPQVRNALTASNLTQTQLATIWFLADVDRDGQLRAEEFILAMHLVEMAKTGR-----PLPLTLPPDLVPPSLRTTKSCDLLNGPVPLIS-----TELIEPELPQKNKSNVSYEDKLRENFQRGNAELEKRRLAL 365 HSP 3 Score: 94.7449 bits (234), Expect = 1.050e-18 Identity = 107/427 (25.06%), Postives = 182/427 (42.62%), Query Frame = 3
Query: 2052 LVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDI---TPKGT-LRKSSECQWAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESII-DLMNASQHSAISLNSVTSNPY----STISDVENNSALYYPQNTVPVNSN-GASTPE---IAQVVIEFNPLSA--EQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYV 3287
L Y A+Y F R DE+T + I ++ E +GWLYG S + G FPE Y + S T+ T + + + S+ + + DI TP T + +++ A A+ W +++ + L F +I +++ ++WW GE KEK GWFP ++V ++ N P S+E++ S L L+ + VA Y Y++ GDL D++ V +++ +WW+G + GLFPSNYVK + ++ +K E V +S + + ++ + I + SN + + + N + +N G TP + QV+ ++ +A +++ Q GQLI + K WWKGE+ FP YV
Sbjct: 749 LTTYRALYPFTARNSDELTLEADCFIQVDESTVRE--TGWLYG--SYSGNRGWFPESYAERCSKDSQTQPTAAETE-STAPSANYPGIPRVDIEGPTPTHTPVSTNTQHSQAVALCEWSAKTD-------SHLGFCKDDIITVLEKQ-ENWWYGEL-------KEK--RGWFPCSHVSVVTTNNTQS------EPLYSSVEEIEVSDSGL-------LDEY--------VALYTYESPESGDLTFSAEDVVLVTEKEGEWWRGCI--GDQTGLFPSNYVKPKEPDTASSAVPSKKPEIAQVTTVSPATTPEQLSLTPGQLIVVLHKNSNSWWLGELQARGKKRKKGWFQSSNVRLLEANSGKITPAPQPLCQVIAMYDYKAANKDEMSFQKGQLITVLNKDNPDWWKGEVAGVTGL-----FPTNYV 1125 HSP 4 Score: 88.9669 bits (219), Expect = 5.206e-17 Identity = 83/313 (26.52%), Postives = 127/313 (40.58%), Query Frame = 3
Query: 1974 SEKNYNEFDKIGISKNPQLNSMPSNELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSECQWAEAIYHWKPE----SNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQ-DWWKGYNRNTNSQGLFPSNYVKIVTSED 2897
SE Y+ ++I +S + L+ Y A+Y +E + ++TF D++++ E W G + + + GLFP YV+ P T S+ A+ KPE + V+ P QLS GQLI ++ N + WW GE L A GK++ GWF + VRLL+ +SG +TP L + +A Y Y+A+ +++ Q G +I VL++ DWWKG GLFP+NYVK+ T D
Sbjct: 913 SEPLYSSVEEIEVSDSGLLDE--------YVALYTYESPESGDLTFSAEDVVLVT-----EKEGEWWRGCIGDQT--GLFPSNYVK--PKEPDTASS--------------------------------------AVPSKKPEIAQVTTVSPATTPEQLSLTPGQLIVVLHKNSNSWWLGE---LQARGKKR-KKGWFQSSNVRLLEA------NSGKITPAPQPLCQV--------------------------IAMYDYKAANKDEMSFQKGQLITVLNKDNPDWWKGEVAGVT--GLFPTNYVKMTTECD 1132
BLAST of Intersectin-1 vs. Ensembl Zebrafish
Match: itsn2b (intersectin 2b [Source:ZFIN;Acc:ZDB-GENE-050309-102]) HSP 1 Score: 496.508 bits (1277), Expect = 6.719e-146 Identity = 334/942 (35.46%), Postives = 482/942 (51.17%), Query Frame = 3
Query: 2433 QLSFETGQLIKIIDT--NLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALYYPQNTVPVNSNGASTPEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLL--ATPCYNTGGMPFVFPLETSSKDKMQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMF 5240
+LSFE+ LI++ ++ W G GK MGWFP++YV + A + P S+++S +T DGS VA Y Y++ GDL GD I V +++ +WW+G + GLFPSNYVK P E + S++S S T ++ V T + GA EQ+ L GQLI I K+PSGWW GE+Q RG++R+ GWFP +V + S + T + S +I +Y+Y+A N DELSF K+ ++ ++DK+ P+WW G L+ GL P NYV+ + S + W D + T ++ +D ++RQ YI ELI+TE+ Y EDLQ V +VF++P+ +SN L+ E+ IF NW +LI S ALK RK G P+ +GDIL S N Y+RFCS Q +G A LQ++ P FK + ++ RC+ MP++S+ LKPMQRIT+Y L +++IL++T ++H D + EA+ A LC VNEGVRE+ENS+RLEW+Q H+QCE ++E L FNS TNCLG RKL+HSG + K KS KEL FLF+DF L A + + G +F S K Q+KMYK P+ LN+V++ S SS F + I V + L+ + N+R WV+++ AS +I TE K E + R + +G+GRLLVT++E L+ SS + KS+P+CE ++G+Q + ++ + T+NPKWN QF IK++ QDVLC+T+ K+ F+P+ FLGR EV V + E + P +RL L EV GE+ VRLDL +F
Sbjct: 952 ELSFESDDLIEVDESVEREQGWLYG-----SWQGK----MGWFPESYVEKQTKSEAPVVAKQALKPQV-----------SISVSSTDASDTTDGSNYEEYVALYTYESPEPGDLTFNEGDTILVSEREGEWWRG--SIGDRAGLFPSNYVK-------------------PKE------------TDTSSLSGKSGTPGKKPEVAQV-----------TTACTAAGA-----------------EQLNLSPGQLILILNKNPSGWWLGELQARGKKRQKGWFPASHV----------------------------KLLGSNSGKSTPATPSV---------GQVIAIYDYTAANEDELSFSKSQVINVLDKSNPDWWKGE--LNGVTGLIPTNYVKLTTDSDPSQQ-----------------------WCADQS-TLESMSPKD-RKRQDYIHELIDTEEKYVEDLQLVLEVFYKPMSESNRLSEAEMNMIFANWRELIQCSSKMLKALKARKKSGGDNMPVHMIGDILTSELSNMQAYIRFCSCQLEGAALLQQRTDQEPDFKTFLKKIATDYRCKGMPLSSFLLKPMQRITRYPLHIKHILENTPESHVDYIQLTEALEKAELLCFEVNEGVREKENSDRLEWIQSHVQCEGIAENLIFNSMTNCLGPRKLLHSGKVFKMKSNKELHAFLFSDFLLFTQAVRQFTSSGTDKLF----SPKSNTQYKMYKTPVFLNEVLVKLPSDPSSDEPVFHISHIDRV------YTLKTENINERTAWVQKIKVASEGFIETEKKKHEKAYQSRSMKGSGIGRLLVTILEATELK--SSKPNGKSNPYCEVTMGSQCYTSRTINDTVNPKWNFNCQFHIKDLYQDVLCITINEKELFSPDAFLGRTEVPVATIKKELE-NKGPSTRRLLLHEVPTGEVWVRLDLQLF 1735 HSP 2 Score: 99.3673 bits (246), Expect = 3.464e-20 Identity = 50/122 (40.98%), Postives = 71/122 (58.20%), Query Frame = 3
Query: 63 NLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLPLSLNPLQTNATTMSYS----GYMPVSNTYNTMPVM 416
++W IT EE+ K D +F L P G++TG +A++ F +S LP VL IW LAD +KDG M+ EF+IAM LIK KL+G +P LP+ + A TM + G MP +P++
Sbjct: 6 SIWAITPEERDKHDQKFDSLLPAQGYVTGEKARNFFIQSGLPQPVLAAIWALADMNKDGKMDRLEFSIAMKLIKMKLQGQNLPTALPIIMKQPPVPAPTMPAAPLGMGAMPNVPMMPNVPIL 127 HSP 3 Score: 93.2041 bits (230), Expect = 3.256e-18 Identity = 67/162 (41.36%), Postives = 96/162 (59.26%), Query Frame = 3
Query: 654 DWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXKKNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAIL 1139
DW +PQ+ R +YR QFN D+ G+LTG + + + + L T LA+IW+LAD+D+DG L +EF +AMHLV+ A+ G+ LP LP L+PPSL+ K LG SM+ + P + E + ++ LT ED+ + N G AELEKRR +L
Sbjct: 232 DWGVPQASRLKYRQQFNSLDKLMSGYLTGPQVRTAMATTLLTQTQLAAIWNLADVDKDGKLKADEFILAMHLVDMAKMGQPLPLALPSDLVPPSLRGK--LGESMNGT-----TPSIYAGLAEDLCEPEPPQKSRNNLTFEDKFKKNMELGNAELEKRRQVL 386 HSP 4 Score: 84.3445 bits (207), Expect = 1.370e-15 Identity = 77/280 (27.50%), Postives = 112/280 (40.00%), Query Frame = 3
Query: 2061 YEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSECQWAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQ-DWWKGYNRNTNSQGLFPSNYVKIVTSED 2897
Y A+Y +E + ++TF GD I+++ E W G + + GLFP YV+ P T+++ SG + GT K E + QL+ GQLI I++ N WW GE L A GK++ GWFP ++V+LL +N S TP+ G + +A Y Y A+ +L+ +I VLD+ DWWKG GL P+NYVK+ T D
Sbjct: 1031 YVALYTYESPEPGDLTFNEGDTILVS-----EREGEWWRGSIGD--RAGLFPSNYVK----PKETDTSSL---------SGKS----------GTPGKKPEVAQV---------TTACTAAGAEQLNLSPGQLILILNKNPSGWWLGE---LQARGKKRQ-KGWFPASHVKLL---GSNSGKSTPATPSVGQV-----------------------------IAIYDYTAANEDELSFSKSQVINVLDKSNPDWWKGELNGVT--GLIPTNYVKLTTDSD 1233 HSP 5 Score: 67.0106 bits (162), Expect = 2.763e-10 Identity = 33/96 (34.38%), Postives = 52/96 (54.17%), Query Frame = 3
Query: 54 NNPNLWFITSEEKVKFDVQFQCLNPV-NGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLPLSLNP 338
P+ W + ++K+ QF L+ + +G++TG Q ++ + L L IW LAD DKDG + ++EF +AMHL+ G +P LP L P
Sbjct: 228 GGPSDWGVPQASRLKYRQQFNSLDKLMSGYLTGPQVRTAMATTLLTQTQLAAIWNLADVDKDGKLKADEFILAMHLVDMAKMGQPLPLALPSDLVP 323 HSP 6 Score: 60.4622 bits (145), Expect = 2.417e-8 Identity = 29/79 (36.71%), Postives = 48/79 (60.76%), Query Frame = 3
Query: 657 WVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGR 893
W I R ++ +F+ +G++TG +A+ +QSGLP LA+IW+LAD+++DG + EF IAM L++ G+
Sbjct: 8 WAITPEERDKHDQKFDSL-LPAQGYVTGEKARNFFIQSGLPQPVLAAIWALADMNKDGKMDRLEFSIAMKLIKMKLQGQ 85
BLAST of Intersectin-1 vs. Ensembl Zebrafish
Match: itsn2a (intersectin 2a [Source:ZFIN;Acc:ZDB-GENE-041014-84]) HSP 1 Score: 489.96 bits (1260), Expect = 4.649e-144 Identity = 328/944 (34.75%), Postives = 483/944 (51.17%), Query Frame = 3
Query: 2547 GWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTES-----AKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYST---------------ISDVEN--------NSALYYPQNTV-PVNSNGAST------PEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYNTGGMPFVFPLETSSKDKM-------QFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMFKK 5246
GWFP++Y + + P + TA S + +Q VA + A L DII VL+++++WW Y +G FP ++V +VT+ NT+S A V V + I+ + + L V Y + +++ E + +P N V P + AS+ PEIAQV + EQ+ L GQLI + K+ + WW GE+Q RG++R+ GWF V ++ + N G +TP P +I MY+Y A N DE+SF K ++ +++K+ P+WW G ++ GLFP NYV+ + S + W D ++ +++ TE E++RQ YI ELI+TE+ Y EDL+ +VF++P+ +S LT E+ IF+NW +LI S AL++RK AG + P+Q VGDIL S + Y+RFCS Q A LQ+K FK + SN RC+ MP++S+ LKPMQRIT+Y LL++NIL++T H D S+++ A+ A LC VNE VRE+ENS+RLEW+Q H+ C+ + E L FNS TNCLG RKL+HSG L K KS KEL LFNDF LL Y + +SS DK+ Q+KMYK P+ LN+V++ S SS + F + I V + L+ + N+R WV+++ AS +I TE LKRE + R + +G+GRLLVTV+E L+ + KS+P+CE ++G Q + ++ P TLNPKW+ QF IK++ QDVLCLTV+ +D F+P+DFLGR EV V + ++ + P+ +RL L EV GE+ VRLDL ++++
Sbjct: 806 GWFPESYAERCSKDSQTQPTAAETESTAPSANYPGIPRVDIEGPTPTHTPVSTNTQHSQAVALCEWSAKTDSHLGFCKDDIITVLEKQENWW--YGELKEKRGWFPCSHVSVVTTN---NTQSDFDMPAGNLLSVLVSLLGLCILAWRRLKCQTLVCLMYVALYTYESPESGDLTFSAEDVVLVTEKEGEWWRGCIGDQTGLFPSNYVKPKEPDTASSAVPSKKPEIAQVTTVSPATTPEQLSLTPGQLIVVLHKNSNSWWLGELQARGKKRKKGWFQSSNVRLLEA----------NSGKITPAPQ---------------------------PLCQVIAMYDYKAANKDEMSFQKGQLITVLNKDNPDWWKGE--VAGVTGLFPTNYVKMTTECDPSQQ-----------------------WCAD-LLSLDSMCTE-ERKRQGYIHELIQTEESYLEDLELAVEVFYKPMAESGRLTEAEISMIFVNWRELIMCSTKLLKALRVRKKTAGERMPVQVVGDILSSELSHMQAYIRFCSCQLNAAALLQQKTDKSLDFKLFLKKIASNYRCKGMPLSSFLLKPMQRITRYPLLIKNILENTPPAHADHSNLQAALEQAEELCSQVNEAVREKENSDRLEWIQNHVLCDGVIEHLVFNSLTNCLGPRKLLHSGKLHKTKSSKELWALLFNDFLLLT---YTSKQF-------SSSPDKLFNPNSNTQYKMYKTPVFLNEVLVKMPSDPSSDDPVFHISHIDRV------YTLKTDTINERTTWVQKIKAASDHFIETEKLKREKAYQARSQKNSGIGRLLVTVLEATELK--PCKPNGKSNPYCELTMGAQCYTSRHQPDTLNPKWSFNCQFFIKDLYQDVLCLTVFERDQFSPDDFLGRTEVPVATIKKDQD-GKGPLVRRLLLHEVPTGEVKVRLDLQLYEQ 1661 HSP 2 Score: 167.548 bits (423), Expect = 6.054e-41 Identity = 131/388 (33.76%), Postives = 191/388 (49.23%), Query Frame = 3
Query: 63 NLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLPLSL----NPLQTNAT--------TMSYSGYMPVSNTYNT-----------------MPVMHNTSHAMSNNNPNNTMAVPHASAFVNYXXXXXXXXXXXXXXXXNAQFGSXXXXXXXXXXSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXKKNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAIL 1139
++W IT EE+ K D QF L P G+++G+QA+ F +S LPT VL IW LAD KDG M+ EF+IAM LIK +L+G +P LP+ + P+ T+++ +S MP+ T +P M N+ + N N T+ P + V+ S T + S++ + + P+ TS DW +PQS R +YR FN D+ G+L+G + + L S L T LA+IW LAD+D+DG L EEF +AMHLVE A++GR LPP L ++ T +N P+ I+ T+ I+ E + KS ++ ED+ R N+ +G AELEKRR L
Sbjct: 6 SIWAITPEERGKHDKQFDSLAPTLGYLSGDQARKFFLQSGLPTSVLADIWALADIGKDGKMDRLEFSIAMKLIKLQLQGQPLPSSLPIIMKQTPTPVMTSSSRFGMGSMPNLSAMSVMPILTPIPTCPVMAPLVPVPTLISGPLPQMPNSISVPALPNGNATLLTPTPAFPVS--------SGLTKSHSLLDLGSSSSNSSSTTSLASNSPK----------TSSSDWAVPQSSRLKYRQLFNSLDKLMSGYLSGPQVRNALTASNLTQTQLATIWFLADVDRDGQLRAEEFILAMHLVEMAKTGR-----PLPLTLPPDLVPPSLRTTKSCDLLNGPVPLIS-----TELIEPELPQKNKSNVSYEDKLRENFQRGNAELEKRRLAL 365 HSP 3 Score: 93.5893 bits (231), Expect = 2.143e-18 Identity = 110/430 (25.58%), Postives = 184/430 (42.79%), Query Frame = 3
Query: 2052 LVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDI---TPKGT-LRKSSECQWAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLC---VARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESII-DLMNASQHSAISLNSVTSNPY----STISDVENNSALYYPQNTVPVNSN-GASTPE---IAQVVIEFNPLSA--EQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYV 3287
L Y A+Y F R DE+T + I ++ E +GWLYG S + G FPE Y + S T+ T + + + S+ + + DI TP T + +++ A A+ W +++ + L F +I +++ ++WW GE KEK GWFP ++V ++ N+ S P L L S L+ + Q L+C VA Y Y++ GDL D++ V +++ +WW+G + GLFPSNYVK + ++ +K E V +S + + ++ + I + SN + + + N + +N G TP + QV+ ++ +A +++ Q GQLI + K WWKGE+ FP YV
Sbjct: 759 LTTYRALYPFTARNSDELTLEADCFIQVDESTVRE--TGWLYG--SYSGNRGWFPESYAERCSKDSQTQPTAAETE-STAPSANYPGIPRVDIEGPTPTHTPVSTNTQHSQAVALCEWSAKTD-------SHLGFCKDDIITVLEKQ-ENWWYGEL-------KEK--RGWFPCSHVSVV---TTNNTQSDFDMPAGNLLSVLVSLLGLCILAWRRL-----KCQTLVCLMYVALYTYESPESGDLTFSAEDVVLVTEKEGEWWRGC--IGDQTGLFPSNYVKPKEPDTASSAVPSKKPEIAQVTTVSPATTPEQLSLTPGQLIVVLHKNSNSWWLGELQARGKKRKKGWFQSSNVRLLEANSGKITPAPQPLCQVIAMYDYKAANKDEMSFQKGQLITVLNKDNPDWWKGEVAGVTGL-----FPTNYV 1151
BLAST of Intersectin-1 vs. Ensembl Xenopus
Match: tpk1 (thiamin pyrophosphokinase 1 [Source:Xenbase;Acc:XB-GENE-958035]) HSP 1 Score: 595.504 bits (1534), Expect = 0.000e+0 Identity = 390/1125 (34.67%), Postives = 571/1125 (50.76%), Query Frame = 3
Query: 2004 IGISKNPQLNSMPSNELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSECQ-WAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIV-------TSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALY------------------YPQNTV-PVNSNGAST----------PEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYNTGGMPFVFPLETSSKDK-------MQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMF 5240
I I+K P + ++VYY A+Y F+ R HDEIT + GDII+++ EP GWL G L + G FP Y ++ P E T + L + TP S+ WA+ W P +N E N D W + + + G+ + + P T + +P G E + + L A YP++A LN DII VL+Q+ WW + +G FP +YVK++ TS D ++ES + V S + M ++ I++ + SN ++ + + + +P N V P +S A + PEIAQV+ + EQ+ L GQLI IR+K+P GWW+GE+Q RG++R+IGWFP YV + +S N+ T + + P +I MY+Y AQN DEL+F K ++ +++K +P+WW G L+ GLFP NYV+ + S + W D + +L+ E++RQ YI ELI TE+ Y DLQ V + F +P+ +S LLT E+ IF+NW +LI + AL++RK ++G K P++ +GDIL + P+ Y+RFCS Q G A +Q+K P+FKE + + RC+ MP++S+ LKPMQR+T+Y L+++NI+++T +NHPD S +++A+ A LC VNEGVRE+ENS+RLEW+Q H+QCE LSE+L FNS TNCLG RK +HSG L K KS KEL GFLFNDF LL + PL +S DK +Q+KMYK PI LN+V++ PT + + IF + + LRA S N+R WV+++ AS YI TE KRE + +R R G+GRL+V +VEGI L+ KS+P+CE ++G+Q H TK + TLNPKWN+ QF IK+++QDVLC+TV+ +D F+P+DFLGR E+ V ++ ++ ++ P+ K L L EV GE+VVRLDL +F
Sbjct: 717 IYINKAPLTFNQGDVKVVYYRALYPFDARSHDEITIQPGDIIMVDESQTGEP--GWLGGELKGKT--GWFPANYAERM---PEGEFPSTTKQAADTTAKPTVHLAPSTPTPAAFTDNSANSNNWADFSSTW-PTNNTDKAE----------------TDNWDTWGAQPSLTVPSAGQHRQRSAFTPATVT------------GSSPSPVLGQGEKV---------------------EGLQAQALYPWRAKKDNHLNFNKNDIITVLEQQDMWW--FGEVQGQKGWFPKSYVKLISGPLRKSTSIDSTSSESPASLKRV----SSPAFKPAMQGEEY--IAMYTYESNEQGDLTFQQGDLIVVIKKDGDWWTGTVGEKTGVFPSNYVRPKDSEAAGSAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKL----------------------------LSPGTNKSTPT--EPPKATSLPPTCQVIGMYDYIAQNDDELAFGKGQVINVLNKEDPDWWKGE--LNGHVGLFPSNYVKLTTDMDPSQQ-----------------------WCAD--LHLLDMLSPTERKRQGYIHELIVTEENYVNDLQLVTETFQKPLLESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEVPEFKEFVKRLAMDPRCKGMPLSSFLLKPMQRVTRYPLIIKNIIENTPENHPDHSHLKQALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQ---------IIKPLGSSGNDKVFSPKSNLQYKMYKTPIFLNEVLVK--LPTDPSGDE----PIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNIVEGIELK--PCRTHGKSNPYCEITMGSQCHITKTIQDTLNPKWNSNCQFFIKDLEQDVLCITVFERDQFSPDDFLGRTEIRVADIKKDQG-SKGPVTKCLLLHEVPTGEIVVRLDLQLF 1701 HSP 2 Score: 198.749 bits (504), Expect = 2.900e-50 Identity = 184/540 (34.07%), Postives = 292/540 (54.07%), Query Frame = 3
Query: 51 LNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLPLSL--NPLQTNATTM------SYSGYMPVS--------------------NTYNTMPVMHNTSHAMSNNNP--NNTMAVPHASAFVNYXXXXXXXXXXXXXXXXNAQFGSXXXXXXXXXXSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXK-KNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAILDEQMRQXXXXXXXXXXXXXXXXXXXXQEKEQARFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKMRLESEKKRLLEMEKRRLEVAIXXXXXXXXXXXXVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYKAGIGSMREHRD 1577
+ N ++W IT EE+ K D QF L P G+ITG+QA++ F +S LP VL +IW LAD + DG M+ EF+IAM LIK KL+GY +P LP ++ P+ +A SG P++ ++ T+P + N + A+ P ++ +P +S+F + GS S+ VP PP+ +W +PQS R +YR FN D+ G LTG +A+ IL+QS LP + LA+IW+L+DID DG LT EEF +AMHL++ A SG+ LP LP +PPS ++ ++ G S+ +S++ + + + + + + ++ +T ED++R N+ +G ELEKRR L EQ R+E+E ERAE ER E+ RQ++E+ R E+++Q+E +R+ E +REE+ R+ E RE + E E++R LE E+ R + +++R +EQE + + +KK L L +KK L+ +++ + L T + I S + R+
Sbjct: 7 IGNLDIWAITVEERAKHDQQFHGLQPTAGYITGDQARNFFLQSGLPQPVLAQIWALADMNNDGRMDQLEFSIAMKLIKLKLQGYPLPSALPSNMLKQPVAMSAAAAAAAAAGGISGIPPLAAVAPVPMPSIPVVGMSPPLVSSVPTVPPLANGAPAVIQPLPAFAHSATLPKSSSF------------------GRSGAGSQMNTKLQKAQSFDVP---TPPPLA------EWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQSQLATIWNLSDIDLDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGLSIMSSVS-----VDQRLPEEPAEEEPQNTDKRLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQDQERKRQQELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNREQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRHEIESTNKSRE 514 HSP 3 Score: 61.6178 bits (148), Expect = 1.794e-8 Identity = 34/89 (38.20%), Postives = 49/89 (55.06%), Query Frame = 3
Query: 624 PPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSG 890
PPI + D W I R ++ QF+ G++TG +A+ +QSGLP LA IW+LAD++ DG + EF IAM L++ G
Sbjct: 5 PPIGNL---DIWAITVEERAKHDQQFHGLQPTA-GYITGDQARNFFLQSGLPQPVLAQIWALADMNNDGRMDQLEFSIAMKLIKLKLQG 89
BLAST of Intersectin-1 vs. Ensembl Xenopus
Match: tpk1 (thiamin pyrophosphokinase 1 [Source:Xenbase;Acc:XB-GENE-958035]) HSP 1 Score: 594.734 bits (1532), Expect = 0.000e+0 Identity = 386/1110 (34.77%), Postives = 565/1110 (50.90%), Query Frame = 3
Query: 2049 ELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSECQ-WAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIV-------TSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALY------------------YPQNTV-PVNSNGAST----------PEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYNTGGMPFVFPLETSSKDK-------MQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMF 5240
++VYY A+Y F+ R HDEIT + GDII+++ EP GWL G L + G FP Y ++ P E T + L + TP S+ WA+ W P +N E N D W + + + G+ + + P T + +P G E + + L A YP++A LN DII VL+Q+ WW + +G FP +YVK++ TS D ++ES + V S + M ++ I++ + SN ++ + + + +P N V P +S A + PEIAQV+ + EQ+ L GQLI IR+K+P GWW+GE+Q RG++R+IGWFP YV + +S N+ T + + P +I MY+Y AQN DEL+F K ++ +++K +P+WW G L+ GLFP NYV+ + S + W D + +L+ E++RQ YI ELI TE+ Y DLQ V + F +P+ +S LLT E+ IF+NW +LI + AL++RK ++G K P++ +GDIL + P+ Y+RFCS Q G A +Q+K P+FKE + + RC+ MP++S+ LKPMQR+T+Y L+++NI+++T +NHPD S +++A+ A LC VNEGVRE+ENS+RLEW+Q H+QCE LSE+L FNS TNCLG RK +HSG L K KS KEL GFLFNDF LL + PL +S DK +Q+KMYK PI LN+V++ PT + + IF + + LRA S N+R WV+++ AS YI TE KRE + +R R G+GRL+V +VEGI L+ KS+P+CE ++G+Q H TK + TLNPKWN+ QF IK+++QDVLC+TV+ +D F+P+DFLGR E+ V ++ ++ ++ P+ K L L EV GE+VVRLDL +F
Sbjct: 736 KVVYYRALYPFDARSHDEITIQPGDIIMVDESQTGEP--GWLGGELKGKT--GWFPANYAERM---PEGEFPSTTKQAADTTAKPTVHLAPSTPTPAAFTDNSANSNNWADFSSTW-PTNNTDKAE----------------TDNWDTWGAQPSLTVPSAGQHRQRSAFTPATVT------------GSSPSPVLGQGEKV---------------------EGLQAQALYPWRAKKDNHLNFNKNDIITVLEQQDMWW--FGEVQGQKGWFPKSYVKLISGPLRKSTSIDSTSSESPASLKRV----SSPAFKPAMQGEEY--IAMYTYESNEQGDLTFQQGDLIVVIKKDGDWWTGTVGEKTGVFPSNYVRPKDSEAAGSAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKL----------------------------LSPGTNKSTPT--EPPKATSLPPTCQVIGMYDYIAQNDDELAFGKGQVINVLNKEDPDWWKGE--LNGHVGLFPSNYVKLTTDMDPSQQ-----------------------WCAD--LHLLDMLSPTERKRQGYIHELIVTEENYVNDLQLVTETFQKPLLESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEVPEFKEFVKRLAMDPRCKGMPLSSFLLKPMQRVTRYPLIIKNIIENTPENHPDHSHLKQALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQ---------IIKPLGSSGNDKVFSPKSNLQYKMYKTPIFLNEVLVK--LPTDPSGDE----PIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNIVEGIELK--PCRTHGKSNPYCEITMGSQCHITKTIQDTLNPKWNSNCQFFIKDLEQDVLCITVFERDQFSPDDFLGRTEIRVADIKKDQG-SKGPVTKCLLLHEVPTGEIVVRLDLQLF 1705 HSP 2 Score: 199.519 bits (506), Expect = 1.662e-50 Identity = 184/542 (33.95%), Postives = 286/542 (52.77%), Query Frame = 3
Query: 54 NNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLP---------LSLNPLQTNATTMSYSGYM-----------------------PVSNTYNTMPVMHNTSHAMSNNNPN--NTMAVPHASAFVNYXXXXXXXXXXXXXXXXNAQFGSXXXXXXXXXXSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXKKNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAILDEQMRQXXXXXXXXXXXXXXXXXXXXQEKEQARFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKMRLESEKKRLLEMEKRRLEVAIXXXXXXXXXXXXVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYKAGIGSMREHRD 1577
N ++W IT EE+ K D QF L P G+ITG+QA++ F +S LP VL +IW LAD + DG M+ EF+IAM LIK KL+GY +P LP +S A SG P+ ++ T+P + N + A+ P ++ +P +S+F + GS S+ VP PP+ +W +PQS R +YR FN D+ G LTG +A+ IL+QS LP + LA+IW+L+DID DG LT EEF +AMHL++ A SG+ LP LP +PPS + ++ G S+ +S++ + + + + + + ++ +T ED++R N+ +G ELEKRR L EQ R+E+E ERAE ER E+ RQ++E+ R E+++Q+E +R+ E +REE+ R+ E RE + E E++R LE E+ R + +++R +EQE + + +KK L L +KK L+ +++ + L T + I S + R+
Sbjct: 10 GNLDIWAITVEERAKHDQQFHGLQPTAGYITGDQARNFFLQSGLPQPVLAQIWALADMNNDGRMDQLEFSIAMKLIKLKLQGYPLPSALPSNMLKQPVAMSAAAAAAAAAGFGMSGISGIPPLAAVAPVPMPSIPVVGMSPPLVSSVPTVPPLANGAPAVIQPLPAFAHSATLPKSSSF------------------GRSGAGSQMNTKLQKAQSFDVP---TPPPLA------EWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQSQLATIWNLSDIDLDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRVRSGSGLSIMSSVS-----VDQRLPEEPAEEEPQNTDKRLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQDQERKRQQELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNREQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRHEIESTNKSRE 519 HSP 3 Score: 63.929 bits (154), Expect = 3.141e-9 Identity = 80/303 (26.40%), Postives = 117/303 (38.61%), Query Frame = 3
Query: 2007 GISKNPQLNSMPSNELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSEC-----QWAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQ-DWWKG--YNRNTNSQ-GLFPSNYVKIVT 2888
G S +P L E + +A+Y + ++ + + F DII + +E W +G + G FP+ YV+ +SGP T ID + S P R SS Q E I + ESN L+F+ G LI +I + DWWTG EK G FP YVR D + A S T + G ++A +S Y A+ L + PG +I + + WW+G R Q G FP+NYVK+++
Sbjct: 887 GSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDIITV-----LEQQDMWWFG--EVQGQKGWFPKSYVKLISGP---LRKSTSIDSTSSES------------PASLKRVSSPAFKPAMQGEEYIAMYTYESNEQ-----GDLTFQQGDLIVVIKKD-GDWWTGTVG-------EKT--GVFPSNYVRPKDSEAAG---SAGKTGSLGKKPEIAQVIAS-------------------------YTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLS 1124
BLAST of Intersectin-1 vs. Ensembl Xenopus
Match: tpk1 (thiamin pyrophosphokinase 1 [Source:Xenbase;Acc:XB-GENE-958035]) HSP 1 Score: 594.734 bits (1532), Expect = 0.000e+0 Identity = 386/1110 (34.77%), Postives = 565/1110 (50.90%), Query Frame = 3
Query: 2049 ELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSECQ-WAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIV-------TSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALY------------------YPQNTV-PVNSNGAST----------PEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYNTGGMPFVFPLETSSKDK-------MQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMF 5240
++VYY A+Y F+ R HDEIT + GDII+++ EP GWL G L + G FP Y ++ P E T + L + TP S+ WA+ W P +N E N D W + + + G+ + + P T + +P G E + + L A YP++A LN DII VL+Q+ WW + +G FP +YVK++ TS D ++ES + V S + M ++ I++ + SN ++ + + + +P N V P +S A + PEIAQV+ + EQ+ L GQLI IR+K+P GWW+GE+Q RG++R+IGWFP YV + +S N+ T + + P +I MY+Y AQN DEL+F K ++ +++K +P+WW G L+ GLFP NYV+ + S + W D + +L+ E++RQ YI ELI TE+ Y DLQ V + F +P+ +S LLT E+ IF+NW +LI + AL++RK ++G K P++ +GDIL + P+ Y+RFCS Q G A +Q+K P+FKE + + RC+ MP++S+ LKPMQR+T+Y L+++NI+++T +NHPD S +++A+ A LC VNEGVRE+ENS+RLEW+Q H+QCE LSE+L FNS TNCLG RK +HSG L K KS KEL GFLFNDF LL + PL +S DK +Q+KMYK PI LN+V++ PT + + IF + + LRA S N+R WV+++ AS YI TE KRE + +R R G+GRL+V +VEGI L+ KS+P+CE ++G+Q H TK + TLNPKWN+ QF IK+++QDVLC+TV+ +D F+P+DFLGR E+ V ++ ++ ++ P+ K L L EV GE+VVRLDL +F
Sbjct: 738 KVVYYRALYPFDARSHDEITIQPGDIIMVDESQTGEP--GWLGGELKGKT--GWFPANYAERM---PEGEFPSTTKQAADTTAKPTVHLAPSTPTPAAFTDNSANSNNWADFSSTW-PTNNTDKAE----------------TDNWDTWGAQPSLTVPSAGQHRQRSAFTPATVT------------GSSPSPVLGQGEKV---------------------EGLQAQALYPWRAKKDNHLNFNKNDIITVLEQQDMWW--FGEVQGQKGWFPKSYVKLISGPLRKSTSIDSTSSESPASLKRV----SSPAFKPAMQGEEY--IAMYTYESNEQGDLTFQQGDLIVVIKKDGDWWTGTVGEKTGVFPSNYVRPKDSEAAGSAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKL----------------------------LSPGTNKSTPT--EPPKATSLPPTCQVIGMYDYIAQNDDELAFGKGQVINVLNKEDPDWWKGE--LNGHVGLFPSNYVKLTTDMDPSQQ-----------------------WCAD--LHLLDMLSPTERKRQGYIHELIVTEENYVNDLQLVTETFQKPLLESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEVPEFKEFVKRLAMDPRCKGMPLSSFLLKPMQRVTRYPLIIKNIIENTPENHPDHSHLKQALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQ---------IIKPLGSSGNDKVFSPKSNLQYKMYKTPIFLNEVLVK--LPTDPSGDE----PIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNIVEGIELK--PCRTHGKSNPYCEITMGSQCHITKTIQDTLNPKWNSNCQFFIKDLEQDVLCITVFERDQFSPDDFLGRTEIRVADIKKDQG-SKGPVTKCLLLHEVPTGEIVVRLDLQLF 1707 HSP 2 Score: 197.208 bits (500), Expect = 9.068e-50 Identity = 184/547 (33.64%), Postives = 288/547 (52.65%), Query Frame = 3
Query: 42 LHMLNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLP---------LSLNPLQTNATTMSYSGYM-----------------------PVSNTYNTMPVMHNTSHAMSNNNP--NNTMAVPHASAFVNYXXXXXXXXXXXXXXXXNAQFGSXXXXXXXXXXSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXK-KNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAILDEQMRQXXXXXXXXXXXXXXXXXXXXQEKEQARFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKMRLESEKKRLLEMEKRRLEVAIXXXXXXXXXXXXVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYKAGIGSMREHRD 1577
+ N ++W IT EE+ K D QF L P G+ITG+QA++ F +S LP VL +IW LAD + DG M+ EF+IAM LIK KL+GY +P LP +S A SG P+ ++ T+P + N + A+ P ++ +P +S+F + GS S+ VP PP+ +W +PQS R +YR FN D+ G LTG +A+ IL+QS LP + LA+IW+L+DID DG LT EEF +AMHL++ A SG+ LP LP +PPS ++ ++ G S+ +S++ + + + + + + ++ +T ED++R N+ +G ELEKRR L EQ R+E+E ERAE ER E+ RQ++E+ R E+++Q+E +R+ E +REE+ R+ E RE + E E++R LE E+ R + +++R +EQE + + +KK L L +KK L+ +++ + L T + I S + R+
Sbjct: 7 IRSFGNLDIWAITVEERAKHDQQFHGLQPTAGYITGDQARNFFLQSGLPQPVLAQIWALADMNNDGRMDQLEFSIAMKLIKLKLQGYPLPSALPSNMLKQPVAMSAAAAAAAAAGFGMSGISGIPPLAAVAPVPMPSIPVVGMSPPLVSSVPTVPPLANGAPAVIQPLPAFAHSATLPKSSSF------------------GRSGAGSQMNTKLQKAQSFDVP---TPPPLA------EWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQSQLATIWNLSDIDLDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGLSIMSSVS-----VDQRLPEEPAEEEPQNTDKRLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQDQERKRQQELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNREQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRHEIESTNKSRE 521 HSP 3 Score: 63.929 bits (154), Expect = 3.089e-9 Identity = 80/303 (26.40%), Postives = 117/303 (38.61%), Query Frame = 3
Query: 2007 GISKNPQLNSMPSNELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSEC-----QWAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQ-DWWKG--YNRNTNSQ-GLFPSNYVKIVT 2888
G S +P L E + +A+Y + ++ + + F DII + +E W +G + G FP+ YV+ +SGP T ID + S P R SS Q E I + ESN L+F+ G LI +I + DWWTG EK G FP YVR D + A S T + G ++A +S Y A+ L + PG +I + + WW+G R Q G FP+NYVK+++
Sbjct: 889 GSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDIITV-----LEQQDMWWFG--EVQGQKGWFPKSYVKLISGP---LRKSTSIDSTSSES------------PASLKRVSSPAFKPAMQGEEYIAMYTYESNEQ-----GDLTFQQGDLIVVIKKD-GDWWTGTVG-------EKT--GVFPSNYVRPKDSEAAG---SAGKTGSLGKKPEIAQVIAS-------------------------YTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLS 1126
BLAST of Intersectin-1 vs. Ensembl Xenopus
Match: tpk1 (thiamin pyrophosphokinase 1 [Source:Xenbase;Acc:XB-GENE-958035]) HSP 1 Score: 594.349 bits (1531), Expect = 0.000e+0 Identity = 386/1110 (34.77%), Postives = 565/1110 (50.90%), Query Frame = 3
Query: 2049 ELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSECQ-WAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIV-------TSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALY------------------YPQNTV-PVNSNGAST----------PEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYNTGGMPFVFPLETSSKDK-------MQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMF 5240
++VYY A+Y F+ R HDEIT + GDII+++ EP GWL G L + G FP Y ++ P E T + L + TP S+ WA+ W P +N E N D W + + + G+ + + P T + +P G E + + L A YP++A LN DII VL+Q+ WW + +G FP +YVK++ TS D ++ES + V S + M ++ I++ + SN ++ + + + +P N V P +S A + PEIAQV+ + EQ+ L GQLI IR+K+P GWW+GE+Q RG++R+IGWFP YV + +S N+ T + + P +I MY+Y AQN DEL+F K ++ +++K +P+WW G L+ GLFP NYV+ + S + W D + +L+ E++RQ YI ELI TE+ Y DLQ V + F +P+ +S LLT E+ IF+NW +LI + AL++RK ++G K P++ +GDIL + P+ Y+RFCS Q G A +Q+K P+FKE + + RC+ MP++S+ LKPMQR+T+Y L+++NI+++T +NHPD S +++A+ A LC VNEGVRE+ENS+RLEW+Q H+QCE LSE+L FNS TNCLG RK +HSG L K KS KEL GFLFNDF LL + PL +S DK +Q+KMYK PI LN+V++ PT + + IF + + LRA S N+R WV+++ AS YI TE KRE + +R R G+GRL+V +VEGI L+ KS+P+CE ++G+Q H TK + TLNPKWN+ QF IK+++QDVLC+TV+ +D F+P+DFLGR E+ V ++ ++ ++ P+ K L L EV GE+VVRLDL +F
Sbjct: 678 KVVYYRALYPFDARSHDEITIQPGDIIMVDESQTGEP--GWLGGELKGKT--GWFPANYAERM---PEGEFPSTTKQAADTTAKPTVHLAPSTPTPAAFTDNSANSNNWADFSSTW-PTNNTDKAE----------------TDNWDTWGAQPSLTVPSAGQHRQRSAFTPATVT------------GSSPSPVLGQGEKV---------------------EGLQAQALYPWRAKKDNHLNFNKNDIITVLEQQDMWW--FGEVQGQKGWFPKSYVKLISGPLRKSTSIDSTSSESPASLKRV----SSPAFKPAMQGEEY--IAMYTYESNEQGDLTFQQGDLIVVIKKDGDWWTGTVGEKTGVFPSNYVRPKDSEAAGSAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKL----------------------------LSPGTNKSTPT--EPPKATSLPPTCQVIGMYDYIAQNDDELAFGKGQVINVLNKEDPDWWKGE--LNGHVGLFPSNYVKLTTDMDPSQQ-----------------------WCAD--LHLLDMLSPTERKRQGYIHELIVTEENYVNDLQLVTETFQKPLLESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEVPEFKEFVKRLAMDPRCKGMPLSSFLLKPMQRVTRYPLIIKNIIENTPENHPDHSHLKQALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQ---------IIKPLGSSGNDKVFSPKSNLQYKMYKTPIFLNEVLVK--LPTDPSGDE----PIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNIVEGIELK--PCRTHGKSNPYCEITMGSQCHITKTIQDTLNPKWNSNCQFFIKDLEQDVLCITVFERDQFSPDDFLGRTEIRVADIKKDQG-SKGPVTKCLLLHEVPTGEIVVRLDLQLF 1647 HSP 2 Score: 197.208 bits (500), Expect = 8.702e-50 Identity = 177/508 (34.84%), Postives = 274/508 (53.94%), Query Frame = 3
Query: 54 NNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLPLSLNPLQTNATTMSYSGYMPVSNTYNTMPVMHNTSHAMSNNNPNNTMAVPHASAFVNYXXXXXXXXXXXXXXXXNAQFGSXXXXXXXXXXSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXKKNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAILDEQMRQXXXXXXXXXXXXXXXXXXXXQEKEQARFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKMRLESEKKRLLEMEKRRLEVAIXXXXXXXXXXXXVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYKAGIGSMREHRD 1577
N ++W IT EE+ K D QF L P G+ITG+QA++ F +S LP VL +IW LAD + DG M+ EF+IAM LIK KL+GY +P LP ++ MS + + + T+P +S+F + GS S+ VP PP+ +W +PQS R +YR FN D+ G LTG +A+ IL+QS LP + LA+IW+L+DID DG LT EEF +AMHL++ A SG+ LP LP +PPS + ++ G S+ +S++ + + + + + + ++ +T ED++R N+ +G ELEKRR L EQ R+E+E ERAE ER E+ RQ++E+ R E+++Q+E +R+ E +REE+ R+ E RE + E E++R LE E+ R + +++R +EQE + + +KK L L +KK L+ +++ + L T + I S + R+
Sbjct: 10 GNLDIWAITVEERAKHDQQFHGLQPTAGYITGDQARNFFLQSGLPQPVLAQIWALADMNNDGRMDQLEFSIAMKLIKLKLQGYPLPSALPSNM---LKQPVAMSAAAAAAAAAGFATLP---------------------KSSSF------------------GRSGAGSQMNTKLQKAQSFDVP---TPPPLA------EWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQSQLATIWNLSDIDLDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRVRSGSGLSIMSSVS-----VDQRLPEEPAEEEPQNTDKRLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQDQERKRQQELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNREQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRHEIESTNKSRE 461 HSP 3 Score: 63.929 bits (154), Expect = 3.147e-9 Identity = 80/303 (26.40%), Postives = 117/303 (38.61%), Query Frame = 3
Query: 2007 GISKNPQLNSMPSNELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSEC-----QWAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQ-DWWKG--YNRNTNSQ-GLFPSNYVKIVT 2888
G S +P L E + +A+Y + ++ + + F DII + +E W +G + G FP+ YV+ +SGP T ID + S P R SS Q E I + ESN L+F+ G LI +I + DWWTG EK G FP YVR D + A S T + G ++A +S Y A+ L + PG +I + + WW+G R Q G FP+NYVK+++
Sbjct: 829 GSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDIITV-----LEQQDMWWFG--EVQGQKGWFPKSYVKLISGP---LRKSTSIDSTSSES------------PASLKRVSSPAFKPAMQGEEYIAMYTYESNEQ-----GDLTFQQGDLIVVIKKD-GDWWTGTVG-------EKT--GVFPSNYVRPKDSEAAG---SAGKTGSLGKKPEIAQVIAS-------------------------YTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLS 1066
BLAST of Intersectin-1 vs. Ensembl Xenopus
Match: tpk1 (thiamin pyrophosphokinase 1 [Source:Xenbase;Acc:XB-GENE-958035]) HSP 1 Score: 594.349 bits (1531), Expect = 0.000e+0 Identity = 392/1102 (35.57%), Postives = 565/1102 (51.27%), Query Frame = 3
Query: 2049 ELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKL----------------SGP-----PSTESTQTKIDVNQNIS------SGFADLFNNDITPKGTLRKSSECQW--AEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALYYPQNTVPVNSNGASTPEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYNTGGMPFVFPLETSSKDK-------MQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMF 5240
++VYY A+Y F+ R HDEIT + GDII+++ EP GWL G L + G FP Y ++ + P PST + D + N + S F +P L + + + A+A+Y W+ + + N L+F +I +++ D WW GE G++ GWFPK+YV+L+ +G L K +S KY +A Y Y+++ GDL Q GD+I V+ + DWW G G+FPSNYV+ SE A G SA S+ P I+ V + S A+ PE Q+ L GQLI IR+K+P GWW+GE+Q RG++R+IGWFP YV + + G+ N T + +I MY+Y AQN DEL+F K ++ +++K +P+WW G L+ GLFP NYV+ + S + W D + +L+ E++RQ YI ELI TE+ Y DLQ V + F +P+ +S LLT E+ IF+NW +LI + AL++RK ++G K P++ +GDIL + P+ Y+RFCS Q G A +Q+K P+FKE + + RC+ MP++S+ LKPMQR+T+Y L+++NI+++T +NHPD S +++A+ A LC VNEGVRE+ENS+RLEW+Q H+QCE LSE+L FNS TNCLG RK +HSG L K KS KEL GFLFNDF LL + PL +S DK +Q+KMYK PI LN+V++ PT + + IF + + LRA S N+R WV+++ AS YI TE KRE + +R R G+GRL+V +VEGI L+ KS+P+CE ++G+Q H TK + TLNPKWN+ QF IK+++QDVLC+TV+ +D F+P+DFLGR E+ V ++ ++ ++ P+ K L L EV GE+VVRLDL +F
Sbjct: 722 KVVYYRALYPFDARSHDEITIQPGDIIMVDESQTGEP--GWLGGELKGKT--GWFPANYAERMPEGEFPSTTKQAADTTAKPTVHLAPSTPTPAAFTDNSANSNNWHRQRSAFTPATVTGSSPSPVLGQGEKVEGLQAQALYPWRAKKD-------NHLNFNKNDIITVLEQQ-DMWWFGEV-----QGQK----GWFPKSYVKLI----------------SGPLR----KSTSSGNKKKY-------------IAMYTYESNEQGDLTFQQGDLIVVIKKDGDWWTG--TVGEKTGVFPSNYVRPKDSE----------AAG-------------------SAGKTGSLGKKP--EIAQV--------------IASYTATGPE--------------QLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYV------------KLLSPGT-----------------------NKTCQ---------VIGMYDYIAQNDDELAFGKGQVINVLNKEDPDWWKGE--LNGHVGLFPSNYVKLTTDMDPSQQ-----------------------WCAD--LHLLDMLSPTERKRQGYIHELIVTEENYVNDLQLVTETFQKPLLESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEVPEFKEFVKRLAMDPRCKGMPLSSFLLKPMQRVTRYPLIIKNIIENTPENHPDHSHLKQALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQ---------IIKPLGSSGNDKVFSPKSNLQYKMYKTPIFLNEVLVK--LPTDPSGDE----PIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNIVEGIELK--PCRTHGKSNPYCEITMGSQCHITKTIQDTLNPKWNSNCQFFIKDLEQDVLCITVFERDQFSPDDFLGRTEIRVADIKKDQG-SKGPVTKCLLLHEVPTGEIVVRLDLQLF 1619 HSP 2 Score: 192.586 bits (488), Expect = 2.141e-48 Identity = 183/552 (33.15%), Postives = 279/552 (50.54%), Query Frame = 3
Query: 21 PNIFQIDLHMLNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLPLSL--NPLQTNATTMSYSGYMPVSNTYNTMPVMHNTSHAMSNNNPNNTMAVPHASAFVNYXXXXXXXXXXXXXXXXNAQFGSXXXXXXXXXXSYAVPQDINKPPIHTVTSD---------------------------DDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXKKNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXV----LTGEDERRANYLKGQAELEKRRAILDEQMRQXXXXXXXXXXXXXXXXXXXXQEKEQARFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKMRLESEKKRLLEMEKRRLEVAIXXXXXXXXXXXXVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYKAGIGSMREHRD 1577
P FQ +L ++W IT EE+ K D QF L P G+ITG+QA++ F +S LP VL +IW LAD + DG M+ EF+IAM LIK KL+GY +P LP ++ P+ + + +SG +S +P + + + P M+ P S+ + + A G+ P + +P+ N P+ S +W +PQS R +YR FN D+ G LTG +A+ IL+QS LP + LA+IW+L+DID DG LT EEF +AMHL++ A SG+ LP LP I P + VSV + + E ++ +T ED++R N+ +G ELEKRR L EQ R+E+E ERAE ER E+ RQ++E+ R E+++Q+E +R+ E +REE+ R+ E RE + E E++R LE E+ R + +++R +EQE + + +KK L L +KK L+ +++ + L T + I S + R+
Sbjct: 9 PTDFQCNL------DIWAITVEERAKHDQQFHGLQPTAGYITGDQARNFFLQSGLPQPVLAQIWALADMNNDGRMDQLEFSIAMKLIKLKLQGYPLPSALPSNMLKQPVAMSFSLFVFSGMSGISG----IPPLAAVAPVPMPSIPVVGMSPPLVSSVPTVPPLANGAPAVIQPLPAFAHSGT---PLSTQTDCFCLPRHHNVSPLSLRNSSAIKMILREKNSFVYDIFYFSSCSPPPLAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQSQLATIWNLSDIDLDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEY----------------------IPPSFSSVSVDQRLPEEPAEEEPVAFFLTVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQDQERKRQQELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNREQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRHEIESTNKSRE 525
BLAST of Intersectin-1 vs. Ensembl Mouse
Match: Itsn1 (intersectin 1 (SH3 domain protein 1A) [Source:MGI Symbol;Acc:MGI:1338069]) HSP 1 Score: 602.438 bits (1552), Expect = 0.000e+0 Identity = 397/1117 (35.54%), Postives = 569/1117 (50.94%), Query Frame = 3
Query: 2028 LNSMPSNELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSECQ-----WAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIV-------TSEDLANTESAKFAEGVPVEEMSESI-------IDLMNASQHSAISLNS-----VTSNPYSTISDVENNSALYYPQNTVPV-NSNGAST----------PEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGD-------HLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLA--TPCYNTGGMPFVFPLETSSKDKMQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMF 5240
+++ S ++VYY A+Y FE R HDEIT + GDI++++ EP GWL G L + G FP Y K+ P E V +++S A TP SSE WA+ W SN E P N D W + + + G+ + + P T + +P G E + + L A YP++A LN D+I VL+Q+ WW + +G FP +YVK++ TS D TES + V +I + +S+ ++ VT + + + +P N V + +S G+ T PEIAQV+ + EQ+ L GQLI IR+K+P GWW+GE+Q RG++R+IGWFP YV + ++ TS I T LP +I MY+Y+AQN DEL+F K I+ +++K +P+WW G +S Q GLFP NYV+ + S + W D + +LT E++RQ YI ELI TE+ Y DLQ V ++F +P+ +S LLT E+ IF+NW +LI + AL++RK ++G K P++ +GDIL + P+ Y+RFCS Q G A +Q+K P FKE + + RC+ MP++S+ LKPMQR+T+Y L+++NIL++T +NHPD S ++ A+ A LC VNEGVRE+ENS+RLEW+Q H+QCE LSE+L FNS TNCLG RK +HSG L K KS KEL GFLFNDF LL T + G VF S K +Q+KMYK PI LN+V++ + S F + I V + LRA S N+R WV+++ AS YI TE KRE + +R R G+GRL+V VVEGI L+ KS+P+CE ++G+Q H TK + TLNPKWN+ QF I++++Q+VLC+TV+ +D F+P+DFLGR E+ V ++ ++ ++ P+ K L L EV GE+VVRLDL +F
Sbjct: 732 ISAQESVKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEP--GWLGGELKGKT--GWFPANYAEKI---PENEVPTPAKPVT-DLTSAPAPKLALRETPAPLPVTSSEPSTTPNNWADFSSTWPSSSN----EKPE-------------TDNWDTWAAQPSLTVPSAGQLRQRSAFTPATAT------------GSSPSPVLGQGEKV---------------------EGLQAQALYPWRAKKDNHLNFNKSDVITVLEQQDMWW--FGEVQGQKGWFPKSYVKLISGPVRKSTSIDTGPTESPASLKRVASPAAKPAIPGEEFIAMYTYESSEQGDLTFQQGDVIVVTKKDGDWWTGTVGDKSGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYAATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKL--------------------------------LSPGTSKITPT-----ELPKTAVQPAVCQVIGMYDYTAQNDDELAFSKGQIINVLNKEDPDWWKGE--VSGQVGLFPSNYVKLTTDMDPSQQ-----------------------WCSD--LHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLTESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILSAQLPHMQPYIRFCSCQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSSGTDKVF----SPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRV------YTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELK--PCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQG-SKGPVTKCLLLHEVPTGEIVVRLDLQLF 1711 HSP 2 Score: 196.438 bits (498), Expect = 1.195e-49 Identity = 191/542 (35.24%), Postives = 288/542 (53.14%), Query Frame = 3
Query: 12 A*FPNIFQIDLHMLNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLP--LSLNPLQ-TNATTMSYSGY--MP-----VSNTYNTMPVMHNTSHAMSNNNPNNTMAVPHASAFVNYXXXXXXXXXXXXXXXXNAQF-----GSXXXXXXXXXXSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXK-----KNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAILDEQMRQXXXXXXXXXXXXXXXXXXXXQEKEQARFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKMRLESEKKRLLEMEKRRLEVAIXXXXXXXXXXXXVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYKAGIGSMREHRD 1577
A FP F L ++W IT EE+ K D QF L P+ GFITG+QA++ F +S LP VL +IW LAD + DG M+ EF+IAM LIK KL+GY++P LP + P+ ++A G MP ++PV+ + +S+ P P A+ + + ++ F GS S+ V + PP +W +PQS R +YR FN D+ G LTG +A+ IL+QS LP LASIW+L+DIDQDG LT EEF +AMHL++ A SG+ LP LP +PPS ++ + +S S +P P + E++ ++K +T ED++R N+ +G ELEKRR L EQ R+E+E ERAE ER E+ RQE+E+ R E+++Q+E +R+ E +REE+ R+ E RE + E E++R LE E+ R + +++R +EQE + +++ L L +KK L+ +++ + L T + I S + R+
Sbjct: 2 AQFPTPFGGSL------DVWAITVEERAKHDQQFLSLKPIAGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSTLPPVMKQQPVAISSAPAFGIGGIASMPPLTAVAPVPMGSIPVVGMSPPLVSSVPP--AAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDV---ASAPPAA------EWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGMSVISSSSVDQRLPEEPSSED---------EQQPEKKLPVTFEDKKRENFERGSVELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEGTVVLKARRKTLEFELEALNDKKHQLEGKLQDIRCRLATQRQEIESTNKSRE 517 HSP 3 Score: 65.0846 bits (157), Expect = 1.017e-9 Identity = 85/352 (24.15%), Postives = 128/352 (36.36%), Query Frame = 3
Query: 2007 GISKNPQLNSMPSNELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSECQWAEAIYHWKPESNVTY-GEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQN------------------------ANHPDSGTVTPTA--------------GSLEDLASKYSSLNLSVKYDLNTFDGSQKL-------------LC--VARYPYQASVLGDLNIQPGDIIEVLDQKQ-DWWKGYNRNTNSQGLFPSNYVKIVTSED 2897
G S +P L E + +A+Y + ++ + + F D+I + Q + W +G + G FP+ YV+ +SGP T ID S +L++ + AI + + TY L+F+ G +I ++ DWWTG T D +G FP YVRL D + A P+ T+ P G L+ K Y G+ K+ +C + Y Y A +L G II VL+++ DWWKG + GLFPSNYVK+ T D
Sbjct: 893 GSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKSDVITVLEQQDM-----WWFG--EVQGQKGWFPKSYVKLISGP---VRKSTSIDTGPTESP-------------ASLKRVASPAAKPAIPGEEFIAMYTYESSEQGDLTFQQGDVI-VVTKKDGDWWTG--TVGDKSGV-------FPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYAATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTELPKTAVQPAVCQVIGMYDYTAQNDDELAFSKGQIINVLNKEDPDWWKG--EVSGQVGLFPSNYVKLTTDMD 1209
BLAST of Intersectin-1 vs. Ensembl Mouse
Match: Itsn1 (intersectin 1 (SH3 domain protein 1A) [Source:MGI Symbol;Acc:MGI:1338069]) HSP 1 Score: 596.66 bits (1537), Expect = 0.000e+0 Identity = 396/1120 (35.36%), Postives = 569/1120 (50.80%), Query Frame = 3
Query: 2028 LNSMPSNELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSG---WLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSECQ-----WAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIV-------TSEDLANTESAKFAEGVPVEEMSESI-------IDLMNASQHSAISLNS-----VTSNPYSTISDVENNSALYYPQNTVPV-NSNGAST----------PEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGD-------HLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLA--TPCYNTGGMPFVFPLETSSKDKMQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMF 5240
+++ S ++VYY A+Y FE R HDEIT + GDI+++ + E +G WL G L + G FP Y K+ P E V +++S A TP SSE WA+ W SN E P N D W + + + G+ + + P T + +P G E + + L A YP++A LN D+I VL+Q+ WW + +G FP +YVK++ TS D TES + V +I + +S+ ++ VT + + + +P N V + +S G+ T PEIAQV+ + EQ+ L GQLI IR+K+P GWW+GE+Q RG++R+IGWFP YV + ++ TS I T LP +I MY+Y+AQN DEL+F K I+ +++K +P+WW G +S Q GLFP NYV+ + S + W D + +LT E++RQ YI ELI TE+ Y DLQ V ++F +P+ +S LLT E+ IF+NW +LI + AL++RK ++G K P++ +GDIL + P+ Y+RFCS Q G A +Q+K P FKE + + RC+ MP++S+ LKPMQR+T+Y L+++NIL++T +NHPD S ++ A+ A LC VNEGVRE+ENS+RLEW+Q H+QCE LSE+L FNS TNCLG RK +HSG L K KS KEL GFLFNDF LL T + G VF S K +Q+KMYK PI LN+V++ + S F + I V + LRA S N+R WV+++ AS YI TE KRE + +R R G+GRL+V VVEGI L+ KS+P+CE ++G+Q H TK + TLNPKWN+ QF I++++Q+VLC+TV+ +D F+P+DFLGR E+ V ++ ++ ++ P+ K L L EV GE+VVRLDL +F
Sbjct: 732 ISAQESVKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKT--GWFPANYAEKI---PENEVPTPAKPVT-DLTSAPAPKLALRETPAPLPVTSSEPSTTPNNWADFSSTWPSSSN----EKPE-------------TDNWDTWAAQPSLTVPSAGQLRQRSAFTPATAT------------GSSPSPVLGQGEKV---------------------EGLQAQALYPWRAKKDNHLNFNKSDVITVLEQQDMWW--FGEVQGQKGWFPKSYVKLISGPVRKSTSIDTGPTESPASLKRVASPAAKPAIPGEEFIAMYTYESSEQGDLTFQQGDVIVVTKKDGDWWTGTVGDKSGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYAATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKL--------------------------------LSPGTSKITPT-----ELPKTAVQPAVCQVIGMYDYTAQNDDELAFSKGQIINVLNKEDPDWWKGE--VSGQVGLFPSNYVKLTTDMDPSQQ-----------------------WCSD--LHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLTESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILSAQLPHMQPYIRFCSCQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSSGTDKVF----SPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRV------YTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELK--PCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQG-SKGPVTKCLLLHEVPTGEIVVRLDLQLF 1716 HSP 2 Score: 196.052 bits (497), Expect = 1.293e-49 Identity = 191/542 (35.24%), Postives = 288/542 (53.14%), Query Frame = 3
Query: 12 A*FPNIFQIDLHMLNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLP--LSLNPLQ-TNATTMSYSGY--MP-----VSNTYNTMPVMHNTSHAMSNNNPNNTMAVPHASAFVNYXXXXXXXXXXXXXXXXNAQF-----GSXXXXXXXXXXSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXK-----KNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAILDEQMRQXXXXXXXXXXXXXXXXXXXXQEKEQARFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKMRLESEKKRLLEMEKRRLEVAIXXXXXXXXXXXXVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYKAGIGSMREHRD 1577
A FP F L ++W IT EE+ K D QF L P+ GFITG+QA++ F +S LP VL +IW LAD + DG M+ EF+IAM LIK KL+GY++P LP + P+ ++A G MP ++PV+ + +S+ P P A+ + + ++ F GS S+ V + PP +W +PQS R +YR FN D+ G LTG +A+ IL+QS LP LASIW+L+DIDQDG LT EEF +AMHL++ A SG+ LP LP +PPS ++ + +S S +P P + E++ ++K +T ED++R N+ +G ELEKRR L EQ R+E+E ERAE ER E+ RQE+E+ R E+++Q+E +R+ E +REE+ R+ E RE + E E++R LE E+ R + +++R +EQE + +++ L L +KK L+ +++ + L T + I S + R+
Sbjct: 2 AQFPTPFGGSL------DVWAITVEERAKHDQQFLSLKPIAGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSTLPPVMKQQPVAISSAPAFGIGGIASMPPLTAVAPVPMGSIPVVGMSPPLVSSVPP--AAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDV---ASAPPAA------EWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGMSVISSSSVDQRLPEEPSSED---------EQQPEKKLPVTFEDKKRENFERGSVELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEGTVVLKARRKTLEFELEALNDKKHQLEGKLQDIRCRLATQRQEIESTNKSRE 517 HSP 3 Score: 65.0846 bits (157), Expect = 1.070e-9 Identity = 85/352 (24.15%), Postives = 128/352 (36.36%), Query Frame = 3
Query: 2007 GISKNPQLNSMPSNELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSECQWAEAIYHWKPESNVTY-GEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQN------------------------ANHPDSGTVTPTA--------------GSLEDLASKYSSLNLSVKYDLNTFDGSQKL-------------LC--VARYPYQASVLGDLNIQPGDIIEVLDQKQ-DWWKGYNRNTNSQGLFPSNYVKIVTSED 2897
G S +P L E + +A+Y + ++ + + F D+I + Q + W +G + G FP+ YV+ +SGP T ID S +L++ + AI + + TY L+F+ G +I ++ DWWTG T D +G FP YVRL D + A P+ T+ P G L+ K Y G+ K+ +C + Y Y A +L G II VL+++ DWWKG + GLFPSNYVK+ T D
Sbjct: 898 GSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKSDVITVLEQQDM-----WWFG--EVQGQKGWFPKSYVKLISGP---VRKSTSIDTGPTESP-------------ASLKRVASPAAKPAIPGEEFIAMYTYESSEQGDLTFQQGDVI-VVTKKDGDWWTG--TVGDKSGV-------FPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYAATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTELPKTAVQPAVCQVIGMYDYTAQNDDELAFSKGQIINVLNKEDPDWWKG--EVSGQVGLFPSNYVKLTTDMD 1214
BLAST of Intersectin-1 vs. Ensembl Mouse
Match: Itsn2 (intersectin 2 [Source:MGI Symbol;Acc:MGI:1338049]) HSP 1 Score: 510.375 bits (1313), Expect = 3.224e-151 Identity = 342/1010 (33.86%), Postives = 512/1010 (50.69%), Query Frame = 3
Query: 2430 NQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYV-RLLDEQNANHPDSGTVTPTA------------GSLEDLAS-KYSSLNLSVKYDLNTF-----------------DGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSES--IIDLMNASQHSAISLNSVTSNPYSTISDVENNSALY------------------YPQNTVP---------VNSNGAST--PEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGDH----LITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYN---TGGMPFVFPLETSSKDKMQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMFKK 5246
+++SF +G +I++ D+ GE L + + K GWFP YV ++L + A P + PT S+ D + +S+L ++ + + + L A + A LN D+I VL+Q+++WW + +G FP +YVK++ ++ E V + S + + I+L S +S ++ E L +P N V + +GAS PEIAQV + EQ+ L GQLI I +K+ SGWW+GE+Q RG++R+ GWFP +V ++ S ++P H +I MY+Y A N DEL+F K ++ +++K++P+WW G + GLFP NYV+ + S + W D + + E++RQ YI ELI+TE+ Y +DLQ V +VF + + +S LT ++ IF+NW +LI ++ AL++RK G K P+Q +GDIL + + Y+RFCS Q G LQ+K FKE + S+ RC+ MP++S+ LKPMQRIT+Y LL+ +IL++T +H D S ++ A+ A LC VNEGVRE+ENS+RLEW+Q H+QCE L+E+L FNS TNCLG RKL+HSG L K KS KEL FLFNDF LL G +F +SK QF+MYK PI LN+V++ + SS F + I V + LR + N+R WV+++ AS YI+TE KRE + R +T+G+GRL+V V+E L+ + + KS+P+CE S+G+Q + T+ + TLNPKWN QF IK++ QDVLCLT++++D F+P+DFLGR EV V + TE+ ++ P +RL L EV GE+ VR DL +F++
Sbjct: 735 DEMSFSSGDIIQV-----DEKTVGEPGWLYGSFQGKF--GWFPCNYVEKVLSSEKALSPKKALLPPTVSLSATSTSSQPPASVTDYHNVSFSNLTVNTTWQQKSAFTRTVSPGSVSPIHGQGQAVENLKAQALCSWTAKKENHLNFSKHDVITVLEQQENWW--FGEVHGGRGWFPKSYVKLIPGNEVQRGEPEALYAAVTKKPTSTAYPVTSTAYPVGEDYIALYSYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGERTGIFPSNYVRPKDQENFGNASKSGASNKKPEIAQVTSAYAASGTEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSER-----------------------------------------TMPTFHAVCQVIAMYDYMANNEDELNFSKGQLINVMNKDDPDWWQGET--NGLTGLFPSNYVKMTTDSDPSQQ-----------------------WCAD--LQALDTMQPTERKRQGYIHELIQTEERYMDDLQLVIEVFQKRMAESGFLTEADMALIFVNWKELIMSNTKLLRALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGATLLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPQSHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHAFLFNDFLLLTYLVRQFAAASGHEKLF----NSKSSAQFRMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRV------YTLRTDNINERTAWVQKIKGASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELK--ACKPNGKSNPYCEVSMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQE-SKGPTTRRLLLHEVPTGEVWVRFDLQLFEQ 1654 HSP 2 Score: 191.045 bits (484), Expect = 5.093e-48 Identity = 139/408 (34.07%), Postives = 208/408 (50.98%), Query Frame = 3
Query: 33 QIDLHMLNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLP--LSLNPLQTNATTMSYS-GYMPVSNTYNTMPVMHNTSHAMSNNN-----------------------PNNT------MAVPHASAFVNYXXXXXXXXXX-XXXXXXNAQFGSXXXXXXXXXXSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXKKNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAILDEQMRQ 1157
Q M PN+W ITSEE+ K D QF L P G+ITG+QA++ F +S LP VL IW L+D +KDG M+ EF+IAM LIK KL+G ++P VLP + P+ + + + G MP + + +P + + +S+ PN T +++P++S+ + +++S ++ S AQ S ++ S N P T +W +PQ R +YR +FN D+ G+L+G +A+ L+QS L T LA+IW+LADID DG L EEF +AMHL + A++G+ LPP L + G S+ NG + E+E Q+K +T ED+R+ANY +G ELEKRR +L EQ ++
Sbjct: 4 QFPTAMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIHQPLPPVAPIATPLSSATSGTSIPPLMMPAPLVPSVSTSSLPNGTASLIQPLSIPYSSSTLPHASSYSLMMGGFGGASIQKAQ--SLIDLGSSSSTSSTASLSGNSPK----TGTSEWAVPQPSRLKYRQKFNSLDKGMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQ-----PLPLTLPPELVPPSFRGGKQVDSV--------NGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQVLMEQQQR 392 HSP 3 Score: 102.449 bits (254), Expect = 4.172e-21 Identity = 128/562 (22.78%), Postives = 228/562 (40.57%), Query Frame = 3
Query: 1797 RMTHRGQKLA------TNRNKVRDLEKKVTSKLLSDIKKLQTSVCEKKNAAI------AILKKQKEPKTAVSEKNYNEFDKIGISKNPQLNSMPSNELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSG------------PPS------TESTQTKIDVNQNISSGFADL-----------FNNDITPKGTLRKSSECQWAEAIYHWKPESNVTY-GEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSA----------------LYYPQNTVPV--NSNGASTP---EIAQVVIEFNPLS--AEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYV 3287
R ++ Q+LA R+K++++E+K ++ KKL+ K +I K+++E + + E+ + + K S ++ LV Y A+Y FE R HDE++F +GDII ++ + EP GWLYG G FP YV K+ PP+ + S+Q V + F++L F ++P + Q A+ + K ++ ++ + N L+F +I +++ ++WW GE G+ GWFPK+YV+L+ G E L + + S Y + + +A Y Y + GDL G+ I V + +WW G G+FPSNYV+ E+ N + + P E+++ S ++A ++ P I ++ N++ ++P + V + S+ + P + QV+ ++ ++ +++ GQLI + K WW+GE FP YV
Sbjct: 625 RESYNTQQLALEQLHKIKRDKLKEIERKRLEQI--QKKKLEDEAARKAKQGKENLWRESIRKEEEEKQKRLQEEKSQDKTQEEERKAEAKQSETASALVNYRALYPFEARNHDEMSFSSGDIIQVDEKTVGEP--GWLYGSFQGK--FGWFPCNYVEKVLSSEKALSPKKALLPPTVSLSATSTSSQPPASVTDYHNVSFSNLTVNTTWQQKSAFTRTVSPGSVSPIHGQGQ---AVENLKAQALCSWTAKKENHLNFSKHDVITVLEQQ-ENWWFGEVHG----GR-----GWFPKSYVKLIPGNEVQR----------GEPEALYAAVTKKPTSTAYPVTSTAYPVGEDYIALYSYSSVEPGDLTFTEGEEILVTQKDGEWWTG--SIGERTGIFPSNYVRPKDQENFGNASKSGASNKKP--EIAQV------TSAYAASGTEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTMPTFHAVCQVIAMYDYMANNEDELNFSKGQLINVMNKDDPDWWQGETNGLTGL-----FPSNYV 1142
BLAST of Intersectin-1 vs. Ensembl Mouse
Match: Itsn2 (intersectin 2 [Source:MGI Symbol;Acc:MGI:1338049]) HSP 1 Score: 510.375 bits (1313), Expect = 3.224e-151 Identity = 342/1010 (33.86%), Postives = 512/1010 (50.69%), Query Frame = 3
Query: 2430 NQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYV-RLLDEQNANHPDSGTVTPTA------------GSLEDLAS-KYSSLNLSVKYDLNTF-----------------DGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSES--IIDLMNASQHSAISLNSVTSNPYSTISDVENNSALY------------------YPQNTVP---------VNSNGAST--PEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGDH----LITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYN---TGGMPFVFPLETSSKDKMQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMFKK 5246
+++SF +G +I++ D+ GE L + + K GWFP YV ++L + A P + PT S+ D + +S+L ++ + + + L A + A LN D+I VL+Q+++WW + +G FP +YVK++ ++ E V + S + + I+L S +S ++ E L +P N V + +GAS PEIAQV + EQ+ L GQLI I +K+ SGWW+GE+Q RG++R+ GWFP +V ++ S ++P H +I MY+Y A N DEL+F K ++ +++K++P+WW G + GLFP NYV+ + S + W D + + E++RQ YI ELI+TE+ Y +DLQ V +VF + + +S LT ++ IF+NW +LI ++ AL++RK G K P+Q +GDIL + + Y+RFCS Q G LQ+K FKE + S+ RC+ MP++S+ LKPMQRIT+Y LL+ +IL++T +H D S ++ A+ A LC VNEGVRE+ENS+RLEW+Q H+QCE L+E+L FNS TNCLG RKL+HSG L K KS KEL FLFNDF LL G +F +SK QF+MYK PI LN+V++ + SS F + I V + LR + N+R WV+++ AS YI+TE KRE + R +T+G+GRL+V V+E L+ + + KS+P+CE S+G+Q + T+ + TLNPKWN QF IK++ QDVLCLT++++D F+P+DFLGR EV V + TE+ ++ P +RL L EV GE+ VR DL +F++
Sbjct: 735 DEMSFSSGDIIQV-----DEKTVGEPGWLYGSFQGKF--GWFPCNYVEKVLSSEKALSPKKALLPPTVSLSATSTSSQPPASVTDYHNVSFSNLTVNTTWQQKSAFTRTVSPGSVSPIHGQGQAVENLKAQALCSWTAKKENHLNFSKHDVITVLEQQENWW--FGEVHGGRGWFPKSYVKLIPGNEVQRGEPEALYAAVTKKPTSTAYPVTSTAYPVGEDYIALYSYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGERTGIFPSNYVRPKDQENFGNASKSGASNKKPEIAQVTSAYAASGTEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSER-----------------------------------------TMPTFHAVCQVIAMYDYMANNEDELNFSKGQLINVMNKDDPDWWQGET--NGLTGLFPSNYVKMTTDSDPSQQ-----------------------WCAD--LQALDTMQPTERKRQGYIHELIQTEERYMDDLQLVIEVFQKRMAESGFLTEADMALIFVNWKELIMSNTKLLRALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGATLLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPQSHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHAFLFNDFLLLTYLVRQFAAASGHEKLF----NSKSSAQFRMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRV------YTLRTDNINERTAWVQKIKGASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELK--ACKPNGKSNPYCEVSMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQE-SKGPTTRRLLLHEVPTGEVWVRFDLQLFEQ 1654 HSP 2 Score: 191.045 bits (484), Expect = 5.093e-48 Identity = 139/408 (34.07%), Postives = 208/408 (50.98%), Query Frame = 3
Query: 33 QIDLHMLNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLP--LSLNPLQTNATTMSYS-GYMPVSNTYNTMPVMHNTSHAMSNNN-----------------------PNNT------MAVPHASAFVNYXXXXXXXXXX-XXXXXXNAQFGSXXXXXXXXXXSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXKKNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAILDEQMRQ 1157
Q M PN+W ITSEE+ K D QF L P G+ITG+QA++ F +S LP VL IW L+D +KDG M+ EF+IAM LIK KL+G ++P VLP + P+ + + + G MP + + +P + + +S+ PN T +++P++S+ + +++S ++ S AQ S ++ S N P T +W +PQ R +YR +FN D+ G+L+G +A+ L+QS L T LA+IW+LADID DG L EEF +AMHL + A++G+ LPP L + G S+ NG + E+E Q+K +T ED+R+ANY +G ELEKRR +L EQ ++
Sbjct: 4 QFPTAMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIHQPLPPVAPIATPLSSATSGTSIPPLMMPAPLVPSVSTSSLPNGTASLIQPLSIPYSSSTLPHASSYSLMMGGFGGASIQKAQ--SLIDLGSSSSTSSTASLSGNSPK----TGTSEWAVPQPSRLKYRQKFNSLDKGMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQ-----PLPLTLPPELVPPSFRGGKQVDSV--------NGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQVLMEQQQR 392 HSP 3 Score: 102.449 bits (254), Expect = 4.172e-21 Identity = 128/562 (22.78%), Postives = 228/562 (40.57%), Query Frame = 3
Query: 1797 RMTHRGQKLA------TNRNKVRDLEKKVTSKLLSDIKKLQTSVCEKKNAAI------AILKKQKEPKTAVSEKNYNEFDKIGISKNPQLNSMPSNELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSG------------PPS------TESTQTKIDVNQNISSGFADL-----------FNNDITPKGTLRKSSECQWAEAIYHWKPESNVTY-GEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSA----------------LYYPQNTVPV--NSNGASTP---EIAQVVIEFNPLS--AEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYV 3287
R ++ Q+LA R+K++++E+K ++ KKL+ K +I K+++E + + E+ + + K S ++ LV Y A+Y FE R HDE++F +GDII ++ + EP GWLYG G FP YV K+ PP+ + S+Q V + F++L F ++P + Q A+ + K ++ ++ + N L+F +I +++ ++WW GE G+ GWFPK+YV+L+ G E L + + S Y + + +A Y Y + GDL G+ I V + +WW G G+FPSNYV+ E+ N + + P E+++ S ++A ++ P I ++ N++ ++P + V + S+ + P + QV+ ++ ++ +++ GQLI + K WW+GE FP YV
Sbjct: 625 RESYNTQQLALEQLHKIKRDKLKEIERKRLEQI--QKKKLEDEAARKAKQGKENLWRESIRKEEEEKQKRLQEEKSQDKTQEEERKAEAKQSETASALVNYRALYPFEARNHDEMSFSSGDIIQVDEKTVGEP--GWLYGSFQGK--FGWFPCNYVEKVLSSEKALSPKKALLPPTVSLSATSTSSQPPASVTDYHNVSFSNLTVNTTWQQKSAFTRTVSPGSVSPIHGQGQ---AVENLKAQALCSWTAKKENHLNFSKHDVITVLEQQ-ENWWFGEVHG----GR-----GWFPKSYVKLIPGNEVQR----------GEPEALYAAVTKKPTSTAYPVTSTAYPVGEDYIALYSYSSVEPGDLTFTEGEEILVTQKDGEWWTG--SIGERTGIFPSNYVRPKDQENFGNASKSGASNKKP--EIAQV------TSAYAASGTEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTMPTFHAVCQVIAMYDYMANNEDELNFSKGQLINVMNKDDPDWWQGETNGLTGL-----FPSNYV 1142
BLAST of Intersectin-1 vs. Ensembl Mouse
Match: Itsn2 (intersectin 2 [Source:MGI Symbol;Acc:MGI:1338049]) HSP 1 Score: 510.375 bits (1313), Expect = 4.984e-151 Identity = 342/1010 (33.86%), Postives = 512/1010 (50.69%), Query Frame = 3
Query: 2430 NQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYV-RLLDEQNANHPDSGTVTPTA------------GSLEDLAS-KYSSLNLSVKYDLNTF-----------------DGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSES--IIDLMNASQHSAISLNSVTSNPYSTISDVENNSALY------------------YPQNTVP---------VNSNGAST--PEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGDH----LITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYN---TGGMPFVFPLETSSKDKMQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMFKK 5246
+++SF +G +I++ D+ GE L + + K GWFP YV ++L + A P + PT S+ D + +S+L ++ + + + L A + A LN D+I VL+Q+++WW + +G FP +YVK++ ++ E V + S + + I+L S +S ++ E L +P N V + +GAS PEIAQV + EQ+ L GQLI I +K+ SGWW+GE+Q RG++R+ GWFP +V ++ S ++P H +I MY+Y A N DEL+F K ++ +++K++P+WW G + GLFP NYV+ + S + W D + + E++RQ YI ELI+TE+ Y +DLQ V +VF + + +S LT ++ IF+NW +LI ++ AL++RK G K P+Q +GDIL + + Y+RFCS Q G LQ+K FKE + S+ RC+ MP++S+ LKPMQRIT+Y LL+ +IL++T +H D S ++ A+ A LC VNEGVRE+ENS+RLEW+Q H+QCE L+E+L FNS TNCLG RKL+HSG L K KS KEL FLFNDF LL G +F +SK QF+MYK PI LN+V++ + SS F + I V + LR + N+R WV+++ AS YI+TE KRE + R +T+G+GRL+V V+E L+ + + KS+P+CE S+G+Q + T+ + TLNPKWN QF IK++ QDVLCLT++++D F+P+DFLGR EV V + TE+ ++ P +RL L EV GE+ VR DL +F++
Sbjct: 762 DEMSFSSGDIIQV-----DEKTVGEPGWLYGSFQGKF--GWFPCNYVEKVLSSEKALSPKKALLPPTVSLSATSTSSQPPASVTDYHNVSFSNLTVNTTWQQKSAFTRTVSPGSVSPIHGQGQAVENLKAQALCSWTAKKENHLNFSKHDVITVLEQQENWW--FGEVHGGRGWFPKSYVKLIPGNEVQRGEPEALYAAVTKKPTSTAYPVTSTAYPVGEDYIALYSYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGERTGIFPSNYVRPKDQENFGNASKSGASNKKPEIAQVTSAYAASGTEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSER-----------------------------------------TMPTFHAVCQVIAMYDYMANNEDELNFSKGQLINVMNKDDPDWWQGET--NGLTGLFPSNYVKMTTDSDPSQQ-----------------------WCAD--LQALDTMQPTERKRQGYIHELIQTEERYMDDLQLVIEVFQKRMAESGFLTEADMALIFVNWKELIMSNTKLLRALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGATLLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPQSHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHAFLFNDFLLLTYLVRQFAAASGHEKLF----NSKSSAQFRMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRV------YTLRTDNINERTAWVQKIKGASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELK--ACKPNGKSNPYCEVSMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQE-SKGPTTRRLLLHEVPTGEVWVRFDLQLFEQ 1681 HSP 2 Score: 190.66 bits (483), Expect = 5.317e-48 Identity = 139/408 (34.07%), Postives = 208/408 (50.98%), Query Frame = 3
Query: 33 QIDLHMLNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLP--LSLNPLQTNATTMSYS-GYMPVSNTYNTMPVMHNTSHAMSNNN-----------------------PNNT------MAVPHASAFVNYXXXXXXXXXX-XXXXXXNAQFGSXXXXXXXXXXSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXKKNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAILDEQMRQ 1157
Q M PN+W ITSEE+ K D QF L P G+ITG+QA++ F +S LP VL IW L+D +KDG M+ EF+IAM LIK KL+G ++P VLP + P+ + + + G MP + + +P + + +S+ PN T +++P++S+ + +++S ++ S AQ S ++ S N P T +W +PQ R +YR +FN D+ G+L+G +A+ L+QS L T LA+IW+LADID DG L EEF +AMHL + A++G+ LPP L + G S+ NG + E+E Q+K +T ED+R+ANY +G ELEKRR +L EQ ++
Sbjct: 4 QFPTAMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIHQPLPPVAPIATPLSSATSGTSIPPLMMPAPLVPSVSTSSLPNGTASLIQPLSIPYSSSTLPHASSYSLMMGGFGGASIQKAQ--SLIDLGSSSSTSSTASLSGNSPK----TGTSEWAVPQPSRLKYRQKFNSLDKGMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQ-----PLPLTLPPELVPPSFRGGKQVDSV--------NGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQVLMEQQQR 392 HSP 3 Score: 102.834 bits (255), Expect = 4.127e-21 Identity = 128/562 (22.78%), Postives = 228/562 (40.57%), Query Frame = 3
Query: 1797 RMTHRGQKLA------TNRNKVRDLEKKVTSKLLSDIKKLQTSVCEKKNAAI------AILKKQKEPKTAVSEKNYNEFDKIGISKNPQLNSMPSNELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSG------------PPS------TESTQTKIDVNQNISSGFADL-----------FNNDITPKGTLRKSSECQWAEAIYHWKPESNVTY-GEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSA----------------LYYPQNTVPV--NSNGASTP---EIAQVVIEFNPLS--AEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYV 3287
R ++ Q+LA R+K++++E+K ++ KKL+ K +I K+++E + + E+ + + K S ++ LV Y A+Y FE R HDE++F +GDII ++ + EP GWLYG G FP YV K+ PP+ + S+Q V + F++L F ++P + Q A+ + K ++ ++ + N L+F +I +++ ++WW GE G+ GWFPK+YV+L+ G E L + + S Y + + +A Y Y + GDL G+ I V + +WW G G+FPSNYV+ E+ N + + P E+++ S ++A ++ P I ++ N++ ++P + V + S+ + P + QV+ ++ ++ +++ GQLI + K WW+GE FP YV
Sbjct: 652 RESYNTQQLALEQLHKIKRDKLKEIERKRLEQI--QKKKLEDEAARKAKQGKENLWRESIRKEEEEKQKRLQEEKSQDKTQEEERKAEAKQSETASALVNYRALYPFEARNHDEMSFSSGDIIQVDEKTVGEP--GWLYGSFQGK--FGWFPCNYVEKVLSSEKALSPKKALLPPTVSLSATSTSSQPPASVTDYHNVSFSNLTVNTTWQQKSAFTRTVSPGSVSPIHGQGQ---AVENLKAQALCSWTAKKENHLNFSKHDVITVLEQQ-ENWWFGEVHG----GR-----GWFPKSYVKLIPGNEVQR----------GEPEALYAAVTKKPTSTAYPVTSTAYPVGEDYIALYSYSSVEPGDLTFTEGEEILVTQKDGEWWTG--SIGERTGIFPSNYVRPKDQENFGNASKSGASNKKP--EIAQV------TSAYAASGTEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERTMPTFHAVCQVIAMYDYMANNEDELNFSKGQLINVMNKDDPDWWQGETNGLTGL-----FPSNYV 1169
BLAST of Intersectin-1 vs. UniProt/SwissProt
Match: sp|Q9WVE9|ITSN1_RAT (Intersectin-1 OS=Rattus norvegicus OX=10116 GN=Itsn1 PE=1 SV=2) HSP 1 Score: 603.208 bits (1554), Expect = 0.000e+0 Identity = 398/1124 (35.41%), Postives = 569/1124 (50.62%), Query Frame = 3
Query: 2028 LNSMPSNELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSECQ-----WAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIV-------TSEDLANTE---------SAKFAEGVPVEEMSESIIDLMNASQHSAISLNS-----VTSNPYSTISDVENNSALYYPQNTVPV-NSNGAST----------PEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGD-------HLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYNTGGMPFVFPLETSSKDK-------MQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMF 5240
+++ S ++VYY A+Y FE R HDEIT + GDI++++ EP GWL G L + G FP Y K+ P E V +++S A TP SSE WA+ W +N E P N D W + + + G+ + + P T + +P G E + + L A YP++A LN D+I VL+Q+ WW + +G FP +YVK++ TS D TE S +P EE + +S+H ++ VT + ++ +P N V + +S G+ T PEIAQV+ + EQ+ L GQLI IR+K+P GWW+GE+Q RG++R+IGWFP YV + ++ TS I T LP +I MY+Y+AQN DEL+F K I+ ++ K +P+WW G +S Q GLFP NYV+ + S + W D + +LT E++RQ YI ELI TE+ Y DLQ V ++F +P+ +S LLT E+ IF+NW +LI + AL++RK ++G K P++ +GDIL + P+ Y+RFCS Q G A +Q+K P FKE + + RC+ MP++S+ LKPMQR+T+Y L+++NIL++T +NHPD S ++ A+ A LC VNEGVRE+ENS+RLEW+Q H+QCE LSE+L FNS TNCLG RK +HSG L K KS KEL GFLFNDF LL PL +SS DK +Q+KMYK PI LN+V++ + S F + I V + LRA S N+R WV+++ AS YI TE KRE + +R R G+GRL+V VVEGI L+ KS+P+CE ++G+Q H TK + TLNPKWN+ QF I++++Q+VLC+TV+ +D F+P+DFLGR E+ V ++ ++ ++ P+ K L L EV GE+VVRLDL +F
Sbjct: 731 ISAQESAKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEP--GWLGGELKGKT--GWFPANYAEKI---PENEIPTPAKPVT-DLTSAPAPKLALRETPAPLPVTSSEPSTTPNNWADFSSTWPSSTN----EKPE-------------TDNWDTWAAQPSLTVPSAGQLRQRSAFTPATAT------------GSSPSPVLGQGEKV---------------------EGLQAQALYPWRAKKDNHLNFNKSDVITVLEQQDMWW--FGEVQGQKGWFPKSYVKLISGPVRKSTSIDTGPTEAPSSLKRVASPAAKPAIPGEEFV--AMYTYESSEHGDLTFQQGDVIVVTKKDGDWWTGTVGETSGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKL--------------------------------LSPGTSKITPT-----ELPKTAVQPAVCQVIGMYDYTAQNDDELAFSKGQIINVLSKEDPDWWKGE--VSGQVGLFPSNYVKLTTDMDPSQQ-----------------------WCSD--LHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLTESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILSAQLPHMQPYIRFCSCQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQ---------ITKPLGSSSTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRV------YTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELK--PCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQG-SKGPVTKCLLLHEVPTGEIVVRLDLQLF 1710 HSP 2 Score: 194.512 bits (493), Expect = 3.133e-48 Identity = 190/536 (35.45%), Postives = 288/536 (53.73%), Query Frame = 3
Query: 12 A*FPNIFQIDLHMLNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLPLSLNPLQTNATTMSYSGY-------MPVSNTYNTMPVMHNTSHAMSNNNPNNTMAVPHASA--FVNYXXXXXXXXXXXXXXXXNAQFGSXXXXXXXXXXSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXK-----KNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAILDEQMRQXXXXXXXXXXXXXXXXXXXXQEKEQARFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKMRLESEKKRLLEMEKRRLEVAIXXXXXXXXXXXXVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYKAGIGSMREHRD 1577
A FP F L ++W IT EE+ K D QFQ L P++GFITG+QA++ F +S LP VL +IW LAD +KDG M+ EF+IAM LIK KL+GY++P LP + Q A S + MP +P+ MS P +VP A+ N + + + + S+ + Q + + + +W +PQS R +YR FN D+ G LTG +A+ IL+QS LP LASIW+L+DIDQDG LT EEF +AMHL++ A SG+ LP LP +PPS ++ + +S SA +P P + E++ ++K +T ED++R N+ +G ELEKRR L EQ R+E+E ERAE ER E+ RQE+E+ R E+++Q+E +R+ E +REE+ R+ E RE + E E++R LE E+ R + +++R ++QE + + +++ L L +KK L+ +++ + L T + I S + R+
Sbjct: 2 AQFPTPFGGSL------DIWAITVEERAKHDQQFQSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNKDGRMDQVEFSIAMKLIKLKLQGYQLPPALPPVMK--QQPAAISSAPAFGIGGMAGMPPLTAVAPVPMGSIPVVGMS---PPLVSSVPQAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASAPAAAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGMSVISSSSADQRLPEEPSSE---------DEQQVEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLTQRNKDQEGIVVLKARRKTLEFELEALNDKKHQLEGKLQDIRCRLATQRQEIESTNKSRE 517
BLAST of Intersectin-1 vs. UniProt/SwissProt
Match: sp|Q9Z0R4|ITSN1_MOUSE (Intersectin-1 OS=Mus musculus OX=10090 GN=Itsn1 PE=1 SV=2) HSP 1 Score: 602.438 bits (1552), Expect = 0.000e+0 Identity = 397/1117 (35.54%), Postives = 569/1117 (50.94%), Query Frame = 3
Query: 2028 LNSMPSNELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSECQ-----WAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIV-------TSEDLANTESAKFAEGVPVEEMSESI-------IDLMNASQHSAISLNS-----VTSNPYSTISDVENNSALYYPQNTVPV-NSNGAST----------PEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGD-------HLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLA--TPCYNTGGMPFVFPLETSSKDKMQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMF 5240
+++ S ++VYY A+Y FE R HDEIT + GDI++++ EP GWL G L + G FP Y K+ P E V +++S A TP SSE WA+ W SN E P N D W + + + G+ + + P T + +P G E + + L A YP++A LN D+I VL+Q+ WW + +G FP +YVK++ TS D TES + V +I + +S+ ++ VT + + + +P N V + +S G+ T PEIAQV+ + EQ+ L GQLI IR+K+P GWW+GE+Q RG++R+IGWFP YV + ++ TS I T LP +I MY+Y+AQN DEL+F K I+ +++K +P+WW G +S Q GLFP NYV+ + S + W D + +LT E++RQ YI ELI TE+ Y DLQ V ++F +P+ +S LLT E+ IF+NW +LI + AL++RK ++G K P++ +GDIL + P+ Y+RFCS Q G A +Q+K P FKE + + RC+ MP++S+ LKPMQR+T+Y L+++NIL++T +NHPD S ++ A+ A LC VNEGVRE+ENS+RLEW+Q H+QCE LSE+L FNS TNCLG RK +HSG L K KS KEL GFLFNDF LL T + G VF S K +Q+KMYK PI LN+V++ + S F + I V + LRA S N+R WV+++ AS YI TE KRE + +R R G+GRL+V VVEGI L+ KS+P+CE ++G+Q H TK + TLNPKWN+ QF I++++Q+VLC+TV+ +D F+P+DFLGR E+ V ++ ++ ++ P+ K L L EV GE+VVRLDL +F
Sbjct: 732 ISAQESVKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEP--GWLGGELKGKT--GWFPANYAEKI---PENEVPTPAKPVT-DLTSAPAPKLALRETPAPLPVTSSEPSTTPNNWADFSSTWPSSSN----EKPE-------------TDNWDTWAAQPSLTVPSAGQLRQRSAFTPATAT------------GSSPSPVLGQGEKV---------------------EGLQAQALYPWRAKKDNHLNFNKSDVITVLEQQDMWW--FGEVQGQKGWFPKSYVKLISGPVRKSTSIDTGPTESPASLKRVASPAAKPAIPGEEFIAMYTYESSEQGDLTFQQGDVIVVTKKDGDWWTGTVGDKSGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYAATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKL--------------------------------LSPGTSKITPT-----ELPKTAVQPAVCQVIGMYDYTAQNDDELAFSKGQIINVLNKEDPDWWKGE--VSGQVGLFPSNYVKLTTDMDPSQQ-----------------------WCSD--LHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLTESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILSAQLPHMQPYIRFCSCQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSSGTDKVF----SPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRV------YTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELK--PCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQG-SKGPVTKCLLLHEVPTGEIVVRLDLQLF 1711 HSP 2 Score: 196.438 bits (498), Expect = 8.357e-49 Identity = 191/542 (35.24%), Postives = 288/542 (53.14%), Query Frame = 3
Query: 12 A*FPNIFQIDLHMLNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLP--LSLNPLQ-TNATTMSYSGY--MP-----VSNTYNTMPVMHNTSHAMSNNNPNNTMAVPHASAFVNYXXXXXXXXXXXXXXXXNAQF-----GSXXXXXXXXXXSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXK-----KNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAILDEQMRQXXXXXXXXXXXXXXXXXXXXQEKEQARFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKMRLESEKKRLLEMEKRRLEVAIXXXXXXXXXXXXVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYKAGIGSMREHRD 1577
A FP F L ++W IT EE+ K D QF L P+ GFITG+QA++ F +S LP VL +IW LAD + DG M+ EF+IAM LIK KL+GY++P LP + P+ ++A G MP ++PV+ + +S+ P P A+ + + ++ F GS S+ V + PP +W +PQS R +YR FN D+ G LTG +A+ IL+QS LP LASIW+L+DIDQDG LT EEF +AMHL++ A SG+ LP LP +PPS ++ + +S S +P P + E++ ++K +T ED++R N+ +G ELEKRR L EQ R+E+E ERAE ER E+ RQE+E+ R E+++Q+E +R+ E +REE+ R+ E RE + E E++R LE E+ R + +++R +EQE + +++ L L +KK L+ +++ + L T + I S + R+
Sbjct: 2 AQFPTPFGGSL------DVWAITVEERAKHDQQFLSLKPIAGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSTLPPVMKQQPVAISSAPAFGIGGIASMPPLTAVAPVPMGSIPVVGMSPPLVSSVPP--AAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDV---ASAPPAA------EWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGMSVISSSSVDQRLPEEPSSED---------EQQPEKKLPVTFEDKKRENFERGSVELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEGTVVLKARRKTLEFELEALNDKKHQLEGKLQDIRCRLATQRQEIESTNKSRE 517 HSP 3 Score: 65.0846 bits (157), Expect = 7.112e-9 Identity = 85/352 (24.15%), Postives = 128/352 (36.36%), Query Frame = 3
Query: 2007 GISKNPQLNSMPSNELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSECQWAEAIYHWKPESNVTY-GEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQN------------------------ANHPDSGTVTPTA--------------GSLEDLASKYSSLNLSVKYDLNTFDGSQKL-------------LC--VARYPYQASVLGDLNIQPGDIIEVLDQKQ-DWWKGYNRNTNSQGLFPSNYVKIVTSED 2897
G S +P L E + +A+Y + ++ + + F D+I + Q + W +G + G FP+ YV+ +SGP T ID S +L++ + AI + + TY L+F+ G +I ++ DWWTG T D +G FP YVRL D + A P+ T+ P G L+ K Y G+ K+ +C + Y Y A +L G II VL+++ DWWKG + GLFPSNYVK+ T D
Sbjct: 893 GSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKSDVITVLEQQDM-----WWFG--EVQGQKGWFPKSYVKLISGP---VRKSTSIDTGPTESP-------------ASLKRVASPAAKPAIPGEEFIAMYTYESSEQGDLTFQQGDVI-VVTKKDGDWWTG--TVGDKSGV-------FPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYAATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTELPKTAVQPAVCQVIGMYDYTAQNDDELAFSKGQIINVLNKEDPDWWKG--EVSGQVGLFPSNYVKLTTDMD 1209
BLAST of Intersectin-1 vs. UniProt/SwissProt
Match: sp|O42287|ITSN1_XENLA (Intersectin-1 OS=Xenopus laevis OX=8355 GN=itsn1 PE=1 SV=2) HSP 1 Score: 595.504 bits (1534), Expect = 1.925e-180 Identity = 383/1110 (34.50%), Postives = 566/1110 (50.99%), Query Frame = 3
Query: 2049 ELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSECQ-WAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIV-------TSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALY------------------YPQNTV-PVNSNGAST----------PEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYNTGGMPFVFPLETSSKDK-------MQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMF 5240
++VYY A+Y F+ R HDEIT + GDII+++ EP GWL G L + G FP Y ++ P +E T + + + + + P S+ WA+ W P +N K+ N D W + + + G+ + + P T + +P G E + + L A YP++A LN D+I VL+Q+ WW + +G FP +YVK++ TS D ++ES + V +I IS+ + SN ++ + + + +P N V P +S A + PEIAQV+ + EQ+ L GQLI IR+K+P GWW+GE+Q RG++R+IGWFP YV + +S N+ T + + P +I MY+Y AQN DEL+F K ++ +++K +P+WW G L+ GLFP NYV+ + S + W D + +L+ E++RQ YI ELI TE+ Y DLQ V + F +P+ +S+LLT E+ IF+NW +LI + AL++RK ++G K P++ +GDIL + P+ Y+RFCS Q G A +Q+K P+FKE + + RC+ MP++S+ LKPMQR+T+Y L+++NI+++T +NHPD S +++A+ A LC VNEGVRE+ENS+RLEW+Q H+QCE LSE+L FNS TNCLG RK +HSG L K KS KEL GFLFNDF LL + PL +S DK +Q+KMYK PI LN+V++ PT + + IF + + LRA S N+R WV+++ AS YI TE KRE + +R R G+GRL+V +VEGI L+ KS+P+CE ++G+Q H TK + TLNPKWN+ QF IK+++QDVLC+TV+ +D F+P+DFLGR E+ V ++ ++ ++ P+ K L L EV GE+VVRLDL +F
Sbjct: 733 KVVYYRALYPFDARSHDEITIEPGDIIMVDESQTGEP--GWLGGELKGKT--GWFPANYAERM---PESEFPSTTKPAAETTAKPTVHVAPSPVAPAAFTNTSTNSNNWADFSSTW-PTNNTD----------------KVESDNWDTWAAQPSLTVPSAGQHRQRSAFTPATVT------------GSSPSPVLGQGEKV---------------------EGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWW--FGEVQGQKGWFPKSYVKLISGPLRKSTSIDSTSSESPASLKRVSSPAFKPAI------QGEEYISMYTYESNEQGDLTFQQGDLIVVIKKDGDWWTGTVGEKTGVFPSNYVRPKDSEAAGSGGKTGSLGKKPEIAQVIASYAATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKL----------------------------LSPGTNKSTPT--EPPKPTSLPPTCQVIGMYDYIAQNDDELAFSKGQVINVLNKEDPDWWKGE--LNGHVGLFPSNYVKLTTDMDPSQQ-----------------------WCAD--LHLLDMLSPTERKRQGYIHELIVTEENYVSDLQLVTETFQKPLLESDLLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEVPEFKEFVKRLAMDPRCKGMPLSSFLLKPMQRVTRYPLIIKNIIENTPENHPDHSHLKQALEKAEELCSQVNEGVREKENSDRLEWIQGHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQ---------IIKPLGSSGNDKVFSPKSNLQYKMYKTPIFLNEVLVK--LPTDPSGDE----PIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNIVEGIELK--PCRTHGKSNPYCEITMGSQCHITKTIQDTLNPKWNSNCQFFIKDLEQDVLCITVFERDQFSPDDFLGRTEIRVADIKKDQG-SKGPVTKCLLLHEVPTGEIVVRLDLQLF 1702 HSP 2 Score: 203.371 bits (516), Expect = 5.215e-51 Identity = 185/539 (34.32%), Postives = 293/539 (54.36%), Query Frame = 3
Query: 54 NNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLP-----------------------LSLNPLQTNA----TTMSYSGYM-PVSNTYNTMPVMHNTSHAMSNNNP--NNTMAVPHASAFVNYXXXXXXXXXXXXXXXXNAQFGSXXXXXXXXXXSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXK-KNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAILDEQMRQXXXXXXXXXXXXXXXXXXXXQEKEQARFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKMRLESEKKRLLEMEKRRLEVAIXXXXXXXXXXXXVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYKAGIGSMREHRD 1577
N ++W IT EE+ K D QF L P G+ITG+QA++ F +S LP VL +IW LAD + DG M+ EF+IAM LIK KL+GY +P +LP + + PL A ++ G P+ ++ T+P + N + A+ ++P ++ +P +S+F + GS S+ VP PP+ +W +P S R +YR FN QD+ G LTG +A+ IL+QS LP + LA+IW+L+DIDQDG LT EEF +AMHL++ A SG+ LP LP +PPS ++ ++ G S+ +S++ + + + + + +K +T ED++R N+ +G ELEKRR L EQ R+E+E ERAE ER E+ RQ++E+ R ++++Q+E +R+ E +REE+ R+ E RE + E E++R LE E+ R + +++R +EQE + + +KK L L +KK L+ +++ + L T + I S + R+
Sbjct: 10 GNLDIWAITVEERAKHDQQFHGLKPTAGYITGDQARNFFLQSGLPQPVLAQIWALADMNNDGRMDQLEFSIAMKLIKLKLQGYPLPSILPSNMLKQPVAMPAAAVAGFGMSGIVGIPPLAAVAPVPMPSIPVVGMSPPLVSSVPTVPPLSNGAPAVIQSHPAFAHSATLPKSSSF------------------GRSVAGSQINTKLQKAQSFDVP----APPLVV-----EWAVPSSSRLKYRQLFNSQDKTMSGNLTGPQARTILMQSSLPQSQLATIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPILPPEYIPPSFRRVRSGSGLSIMSSVS-----VDQRLPEEPEEEEPQNADKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQDQERKRQQDLEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNREQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRHEIESTNKSRE 516 HSP 3 Score: 66.6254 bits (161), Expect = 2.984e-9 Identity = 77/299 (25.75%), Postives = 119/299 (39.80%), Query Frame = 3
Query: 2007 GISKNPQLNSMPSNELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSECQWAEAIYHWKPESNVTY-GEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQ-DWWKG--YNRNTNSQ-GLFPSNYVKIVT 2888
G S +P L E + +A+Y + ++ + + F D+I + +E W +G + G FP+ YV+ +SGP T ID + S +L++ S + AI + S TY L+F+ G LI +I + DWWTG EK G FP YVR D + A SG T + G ++A +S Y A+ L + PG +I + + WW+G R Q G FP+NYVK+++
Sbjct: 884 GSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITV-----LEQQDMWWFG--EVQGQKGWFPKSYVKLISGP---LRKSTSIDSTSSESP-------------ASLKRVSSPAFKPAIQGEEYISMYTYESNEQGDLTFQQGDLIVVIKKD-GDWWTGTVG-------EKT--GVFPSNYVRPKDSEAAG---SGGKTGSLGKKPEIAQVIAS-------------------------YAATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLS 1121
BLAST of Intersectin-1 vs. UniProt/SwissProt
Match: sp|Q15811|ITSN1_HUMAN (Intersectin-1 OS=Homo sapiens OX=9606 GN=ITSN1 PE=1 SV=3) HSP 1 Score: 594.349 bits (1531), Expect = 6.012e-180 Identity = 399/1145 (34.85%), Postives = 583/1145 (50.92%), Query Frame = 3
Query: 1938 ILKKQKEPKTAVSEKNYNEFDKIGISKNPQLNSMPSNELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSG---WLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSECQ-----WAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIV-------TSEDLANTESAKFAEGV------PVEEMSESI-IDLMNASQHSAISLNS-----VTSNPYSTISDVENNSALYYPQNTVPV-NSNGAST----------PEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGD--HLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLA--TPCYNTGGMPFVFPLETSSKDKMQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMF 5240
+ + +EP + ++ +K ++ + Q N ++VYY A+Y FE R HDEIT + GDI+++ + E +G WL G L + G FP Y K+ P E V + S+ L + TP SSE WA+ W +N E P N D W + + + G+ + + P T + +P G E + + L A YP++A LN D+I VL+Q+ WW + +G FP +YVK++ TS D ++ES + V PV E I + +S+ ++ VT + + A +P N V + +S G+ T PEIAQV+ + EQ+ L GQLI IR+K+P GWW+GE+Q RG++R+IGWFP YV + ++ TS I T + +I MY+Y+AQN DEL+F+K I+ +++K +P+WW G ++ Q GLFP NYV+ + S + W D + +LT E++RQ YI ELI TE+ Y DLQ V ++F +P+ +S LLT E+ IF+NW +LI + AL++RK ++G K P++ +GDIL + P+ Y+RFCS Q G A +Q+K P FKE + + RC+ MP++S+ LKPMQR+T+Y L+++NIL++T +NHPD S ++ A+ A LC VNEGVRE+ENS+RLEW+Q H+QCE LSE+L FNS TNCLG RK +HSG L K KS KEL GFLFNDF LL T + G VF S K +Q+KMYK PI LN+V++ + S F + I V + LRA S N+R WV+++ AS YI TE KRE + +R R G+GRL+V VVEGI L+ KS+P+CE ++G+Q H TK + TLNPKWN+ QF I++++Q+VLC+TV+ +D F+P+DFLGR E+ V ++ ++ ++ P+ K L L EV GE+VVRLDL +F
Sbjct: 708 LFHQHQEPAKPAVQAPWSTAEKGPLTISAQENV----KVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKT--GWFPANYAEKI---PENEVPAPVKPVTDSTSAPAPKLALRE-TPAPLAVTSSEPSTTPNNWADFSSTWPTSTN----EKPE-------------TDNWDAWAAQPSLTVPSAGQLRQRSAFTPATAT------------GSSPSPVLGQGEKV---------------------EGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWW--FGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKAGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKL--------------------------------LSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGE--VNGQVGLFPSNYVKLTTDMDPSQQ-----------------------WCSD--LHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILSAQLPHMQPYIRFCSRQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSSGTDKVF----SPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRV------YTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELK--PCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQG-SKGPVTKCLLLHEVPTGEIVVRLDLQLF 1718 HSP 2 Score: 202.986 bits (515), Expect = 6.519e-51 Identity = 194/540 (35.93%), Postives = 290/540 (53.70%), Query Frame = 3
Query: 12 A*FPNIFQIDLHMLNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLP--LSLNPLQTN---ATTMSYSGYMPVSNTYNTMPVMHNTSHAMSNNNPNN--TMAVPH-ASAFVNYXXXXXXXXXXXXXXXXNAQF-----GSXXXXXXXXXXSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXK-----KNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAILDEQMRQXXXXXXXXXXXXXXXXXXXXQEKEQARFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKMRLESEKKRLLEMEKRRLEVAIXXXXXXXXXXXXVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYKAGIGSMREHRD 1577
A FP F L ++W IT EE+ K D QF L P++GFITG+QA++ F +S LP VL +IW LAD + DG M+ EF+IAM LIK KL+GY++P LP + P+ + A M MP +P+ MS ++ T AVP A+ + + ++ F GS S+ V + PP+ +W +PQS R +YR FN D+ G LTG +A+ IL+QS LP LASIW+L+DIDQDG LT EEF +AMHL++ A SG+ LP LP +PPS ++ + +S S +P P+ +++ ++K +T ED++R N+ +G ELEKRR L EQ R+E+E ERAE ER E+ RQE+E+ R E+++Q+E +R+ E +REE+ R+ E RE + E E++R LE E+ R + +++R +EQE + + +KK L L +KK L+ +++ + L T + I S + R+
Sbjct: 2 AQFPTPFGGSL------DIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPTLVSSVPTAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDV---ASVPPVA------EWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLE--------DEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRE 518
BLAST of Intersectin-1 vs. UniProt/SwissProt
Match: sp|Q9NZM3|ITSN2_HUMAN (Intersectin-2 OS=Homo sapiens OX=9606 GN=ITSN2 PE=1 SV=3) HSP 1 Score: 521.931 bits (1343), Expect = 3.949e-154 Identity = 347/1008 (34.42%), Postives = 524/1008 (51.98%), Query Frame = 3
Query: 2430 NQLSFETGQLIKIIDTNLDD--WWTGETTALDANGKEKIIMGWFPKTYV---------RLLDEQNANHPDSGTVTPTAGSLEDLAS------------KYSSLNLSVKYD-------------LNTFDGS----QKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGV---PVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALY----------YPQNTV---------PVNSNGAST--PEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYN---TGGMPFVFPLETSSKDKMQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMFKK 5246
+++SF +G +I++ + + + W G + N GWFP YV + + + A P + +++ T+ S E L+S +S+L ++ + ++ G + L A + A LN DII VL+Q+++WW + +G FP +YVKI+ ++ E V P + + +S++ +T I + + + +P N V + +GAS PEIAQV + +EQ+ L GQLI I +K+ SGWW+GE+Q RG++R+ GWFP +V ++ SS +T + P +I MY+Y+A N DELSF K ++ +++K++P+WW G ++ GLFP NYV+ + S + W D T + + E++RQ YI ELI+TE+ Y DLQ V +VF + + +S LT E+ IF+NW +LI ++ AL++RK G K P+Q +GDIL + + Y+RFCS Q G A LQ+K FKE + S+ RC+ MP++S+ LKPMQRIT+Y LL+ +IL++T ++H D S ++ A+ A LC VNEGVRE+ENS+RLEW+Q H+QCE L+E+L FNS TNCLG RKL+HSG L K KS KEL GFLFNDF LL + G +F SSK QFKMYK PI LN+V++ + SS F + I V + LR + N+R WV+++ AS YI+TE KRE + R +T+G+GRL+V V+E L+ + + KS+P+CE S+G+Q + T+ + TLNPKWN QF IK++ QDVLCLT++++D F+P+DFLGR E+ V + TE+ ++ P+ +RL L EV GE+ VR DL +F++
Sbjct: 774 DEMSFNSGDIIQVDEKTVGEPGWLYG---SFQGN------FGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWW--FGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVNKKPTSAAYSVGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLL----------------------------------GPSSERATPAFH---PVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGE--INGVTGLFPSNYVKMTTDSDPSQQ-----------------------WCAD-LQTLDT-MQPIERKRQGYIHELIQTEERYMADLQLVVEVFQKRMAESGFLTEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPESHADHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLF----SSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRV------YTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELK--ACKPNGKSNPYCEISMGSQSYTTRTIQDTLNPKWNFNCQFFIKDLYQDVLCLTLFDRDQFSPDDFLGRTEIPVAKIRTEQE-SKGPMTRRLLLHEVPTGEVWVRFDLQLFEQ 1693 HSP 2 Score: 192.586 bits (488), Expect = 1.183e-47 Identity = 185/536 (34.51%), Postives = 284/536 (52.99%), Query Frame = 3
Query: 33 QIDLHMLNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLP--LSLNPLQTNATTMSYS-GYMPVSNTYNTMP---VMHNTSHAMSNNN-------------------PNNT------MAVPHASAFVNYXXXXXXXXXX-XXXXXXNAQFGSXXXXXXXXXXSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXKKNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAILDEQMRQXXXXXXXXXXXXXXXXXXXXQEKEQARFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKMRLESEKKRLLEMEKRRLEVAIXXXXXXXXXXXXVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYK 1544
Q M PN+W ITSEE+ K D QF L P G+ITG+QA++ F +S LP VL IW L+D +KDG M+ EF+IAM LIK KL+G ++P VLP + P+ + + + G MP + +P + + S A S N PN T + +P++S+ + + +S ++ S AQ S ++ S N P T +W +PQ R +YR +FN D++ G+L+G +A+ L+QS L T LA+IW+LAD+D DG L EEF +AMHL + A++G+ LP LP L S I+ I NG + E+E Q+K +T ED+R+ANY +G ELEKRR L EQ ++E E + KE+ E ER ++ QE+E + E+++++E +R+ E +REE+ R+ E RE + E E++R LE E+ R + ++++ +EQE + +NSKK NL L K + + R++ ++ +T K
Sbjct: 4 QFPTAMNGGPNMWAITSEERTKHDRQFDNLKPSGGYITGDQARNFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQPLPPAAPITSLSSATSGTNLPPLMMPTPLVPSVSTSSLPNGTASLIQPLPIPYSSSTLPHGSSYSLMMGGFGGASIQKAQ--SLIDLGSSSSTSSTASLSGNSPK----TGTSEWAVPQPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADVDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPEL------------VPPSFRGGKQIDSI-NGTLPSYQKMQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEWERIRRQELLNQKNREQEEIVRLNSKKKNLHLELEALNGKHQQISGRLQDVRLKKQTQK 520 HSP 3 Score: 97.8265 bits (242), Expect = 9.260e-19 Identity = 118/480 (24.58%), Postives = 196/480 (40.83%), Query Frame = 3
Query: 2052 LVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSG--------------PP-----STESTQTKIDVNQNISS------GFADL-----------FNNDITPKGTLRKSSECQW-----AEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLN---LSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVP-VEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSA----------------LYYPQNTVPV--NSNGASTPE---IAQVVIEFNPLS--AEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYV 3287
LV Y A+Y FE R HDE++F +GDII ++ + EP GWLYG + G FP YV K+ PP +T ++ + NQ S F++L F ++P + Q A+A+ W + + N L+F +I +++ ++WW GE G+ GWFPK+YV+++ P S E+ + Y+++N S Y + G + +A YPY + GDL G+ I V + +WW G + G+FPSNYVK E + + + P + +++ + + AS +SL P I ++ N++ ++P + V + S+ +TP + QV+ ++ + +++ GQLI + K WW+GEI FP YV
Sbjct: 759 LVNYRALYPFEARNHDEMSFNSGDIIQVDEKTVGEP--GWLYGSFQG--NFGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKD-------NHLNFSKHDIITVLEQQ-ENWWFGEVHG----GR-----GWFPKSYVKII-------PGSEV------KREEPEALYAAVNKKPTSAAYSV----GEE---YIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTG--SIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYV----ASGSEQLSL-----APGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATPAFHPVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGEINGVTGL-----FPSNYV 1181
BLAST of Intersectin-1 vs. TrEMBL
Match: A0A452SWJ9 (Intersectin 1 OS=Ursus americanus OX=9643 GN=ITSN1 PE=4 SV=1) HSP 1 Score: 766.533 bits (1978), Expect = 0.000e+0 Identity = 612/1816 (33.70%), Postives = 907/1816 (49.94%), Query Frame = 3
Query: 12 A*FPNIFQIDLHMLNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLPLSLNPLQTNATTMSYSGYMPVSNTYNTMPVMHNTSHA----MSNNNPNNTMAVPHASAFVNYXXXXXXXXXXXXXXXXNAQFGSXXXXXXXXXXSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXKKNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAILDEQMRQXXXXXXXXXXXXXXXXXXXXQEKEQARFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKMRLESEKKRLLEMEKRRLEVAIXXXXXXXXXXXXVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYKAGIGSMREHRDKLIDTTDCXXXXXXXXXXXXSRCQREKXXXXXXXXXXXXXDPVLEQIKTVQ--ALKSSHXXXXXXXXXXXXXXXQRMTHRGQKLATNRNKVRDLEKKVTSKL---------LSDIKKLQTSVCEKKNAAIAILK-KQKEPKTAVSEKNYNEFDKIGISKNPQLNSMPSNELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLS----------GPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSECQWAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIV-------TSEDLANTESAKFAEGVPVEEMSESII-DLMNASQHSA----ISLNSVTSNPYSTISDVENNSALY------------------YPQNTVPV-NSNGAST----------PEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELND-SLPGD-HLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYNTG--GMPFVFPLETSSKDKMQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMF 5240
A FP F L ++W IT EE+ K D QF L P++GFITG+QA++ F +S LP VL +IW LAD + DG M+ EF+IAM LIK KL+GY++P LP + Q A +M G +PV + P++ + A ++N P + SAF + + S S S + GS S+ V + PP+ +W +PQS R +YR FN D+ G LTG +A+ IL+QS LP LASIW+L+DIDQDG LT EEF +AMHL++ A S GT S K +LT ED++R N+ +G ELEKRR L EQ R+E+E ERAE ER E+ RQE+E+ R E+++Q+E +R+ E +REE+ R+ E RE + E E++R LE E+ R + +++R +EQE + + +KK L L +KK L+ +++ + L T + I S + R+ I L+++L+ A+ L R EKQ L +Q+K VQ +L +K + + Q++ R+++ ++EK+ SKL L +++++ +K +I + KQKE E+ E +K +N ++VYY A+Y FE R HDEIT + GDI++++ EP GWL G L + G FP Y K+ GP T + K+ V + + TP WA+ W +N E P N D W + + + G+ + + P T + +P G E + + L A YP++A LN D+I VL+Q+ WW + +G FP +YVK++ TS D ++ES + V +I + ++ SQ+ + I++ + S+ ++ + + L +P N V + +S G+ T PEIAQV+ + EQ+ L GQLI IR+K+P GWW+GE+Q RG++R+IGWFP YV + ++ TS I T + P +I +Y+YSAQN DEL+F K I+ +++K++P+WW G ++ Q GLFP NYV+ + S + W D + +LT E++RQ YI ELI TE+ Y DLQ V ++F +P+ +S LLT E +F+NW +LI + AL++RK ++G K P++ +GDIL + P+ Y+RFCS Q G A +Q+K P FK+ + + RC+ MP++S+ LKPMQR+T+Y L+++NIL++T +NHPD S ++ A+ A LC VNEGVRE+ENS+RLEW+Q H+QCE LSE+L FNS TNCLG RK +HSG L K KS KEL GFLFNDF LL G G VF S K +Q+KMYK PI LN+V++ PT + + IF + + LRA S N+R WV+++ AS YI TE KRE + +R R G+GRL+V VVEGI L+ KS+P+CE ++G+Q H TK + TLNPKWN+ QF IK+++Q+VLC+TV+ +D F+P+DFLGR E+ V ++ ++ ++ P+ K L L EV GE+VVRLDL +F
Sbjct: 2 AQFPTPFGGSL------DIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMK-QQPVAISMPM-GSIPVVGM--SPPLVSSVPTAAVPPLANGAPP---VIQPLSAFAH-----PAATLPKSSSFSRSGPGSQLNTKLQKAQSFDV---ASVPPVA------EWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMS-----------------------GTFGS---------------------------YKKLLTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQEAQQMLGRLIPEKQILN-------------DQLKQVQQNSLHRDSLLTLKRALEAKELGRQQL----------RDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSIEAERLKQKE-----QERKIIELEKQKEEEN--------VKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEP--GWLGGELKGKT--GWFPANYAEKIPENEVPAPVKPGPDPTSTPAPKLAVRETPAPVAVTAPEPSTTPNN---------WADFSSTWPTSTN----EKPE-------------TDNWDAWAAQPSLTVPSAGQLRQRSAFTPATAT------------GSSPSPVLGQGEKV---------------------EGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWW--FGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVSSPAAKPAISGEALSVSQYFSPPEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKSGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIACYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKL--------------------------------LSPGTSKITPTEPPKPTAFPAVCQVIGVYDYSAQNDDELAFSKGQIINVLNKDDPDWWRGE--VNGQVGLFPSNYVKLTADTDPSQQ-----------------------WCSD--LHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEGAMVFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEAPDFKDFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQIIKPLGSSGTDKVF----SPKSNLQYKMYKTPIFLNEVLVK--LPTDPSGDE----PIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELK--PCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIKDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQG-SKGPVTKCLLLHEVPTGEIVVRLDLQLF 1567
BLAST of Intersectin-1 vs. TrEMBL
Match: A0A452HDK1 (Intersectin 1 OS=Gopherus agassizii OX=38772 GN=ITSN1 PE=4 SV=1) HSP 1 Score: 762.296 bits (1967), Expect = 0.000e+0 Identity = 600/1809 (33.17%), Postives = 907/1809 (50.14%), Query Frame = 3
Query: 54 NNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLPLSLNPLQTNATTMSYSGY-------MPVSNTYNTMPVMHNTSHAMSNNNPNN--TMAVP---HASAFVNYXXXXXXXXXXXXXXXXNAQFGSXXXXXXXXXXSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXKKNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAILDEQMRQXXXXXXXXXXXXXXXXXXXXQEKEQARFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKMRLESEKKRLLEMEKRRLEVAIXXXXXXXXXXXXVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYKAGIGSMREHRD-KLIDTTDCXXXXXXXXXXXXSRCQREKXXXXXXXXXXXXXDPVLEQIKTVQALKSSHXXXXXXXXXXXXXXXQRMTHRGQKLATNRNKVRDLEKKVTSKL---------LSDIKKL--QTSVCEKKNAAIAILKKQKEPKTAVSEKNYNEFDKIGISKNP-QLNSMPSNELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISS------------------------GFADLFNNDI---------TPKGTLRKSS-----------ECQWAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALYYPQNTVPVNSNGASTPEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYNTGGMPFVFPLETSSKDK-------MQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMFKK 5246
N ++W IT EE+ K D QF L P +GFITG+QA++ F +S LP VL +IW LAD + DG M+ EF+IAM LIK KL+GY++P LP ++ Q G+ MP +P+ MS ++ T AVP + + V + L N F ++ + + + + + P +V S +W +PQS R +YR FN D+ G LTG +A+ IL+QS LP T LA+IW+L+DIDQDG LT EEF +AMHL++ A SG+ LP LP I P + VSV +S+ S V T ED++R N+ +G ELEKRR L EQ R+E+E ERAE ER E+ RQE+E+ R E+++Q+E +R+ E +REE+ R+ E RE + E E++R LE E+ R + +++R +EQE + + +KK L L +KK L+ +++ + L + I S + R+ ++ + T ++ Q+ + L +SH + +++ + + R+++ ++EK+ SKL L +++++ + + ++KN + LK++++ + F + I K P +++ ++VYY A+Y FE R HDEI+ + GDI++++ EP GWL G L + G FP Y K+ P + T K V+ ++S +AD + + TP T+ SS E A+A+Y W+ + + N L+F +I +++ D WW GE G++ GWFPK+YV+L+ SG + + A S+ + L T +A Y Y++S GDL Q GD+I V + DWW G + + G+FPSNYV++ E VP +A S+ P I+ V + S A+ PE Q+ L GQLI IR+K+P GWW+GE+Q RG++R+IGWFP YV + + G++ +I MY+Y+AQN DEL+F+K I+ +++K++P+WW G ++ Q GLFP NYV+ + S + W D + +LT E++RQ YI ELI TE+ Y DLQ V ++F +P+ +S LLT E+ IF+NW +LI + AL++RK ++G + P++ +GDIL + P+ Y+RFCS Q G A +Q+K +FKE + + RC+ MP++S+ LKPMQR+T+Y L+++NI+++T +NHPD S ++ A+ A LC VNEGVRE+ENS+RLEW+Q H+QCE LSE+L FNS TNCLG RK +HSG L K KS KEL GFLFNDF LL + PL +SS DK +Q+KMYK PI LN+V++ PT + + IF + + LRA S N+R WV+++ AS YI TE KRE + +R R G+GRL+V +VEGI L+ KS+P+CE ++G+Q H TK + TLNPKWN+ QF IK+++QDVLC+TV+ +D F+P+DFLGR E+ V ++ ++ ++ P+ K L L EV GE+VVRLDL K
Sbjct: 11 GNLDIWAITVEERAKHDQQFHSLKPTSGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQMEFSIAMKLIKLKLQGYQLPSALPSAMK--QPPIAIPGAPGFGIGSIVSMPSLTAVAPVPMASIPVVGMSPPLVSSVPTAAVPPLANGAPAVIQPLPAFAHPEQVQLLHMNLNFSLSSCSLKLLSVT-----EFSLSPNSSVPSVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQTQLATIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEY----------------------IPPSFSSVSVDQSLISPFTFSCVKV-TFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLSIQRHEIESTNKSRELRIAEITHLQQQL---------------------------------QVSMLVFLINSHLTICLKTGDSLLTIKRALEAKELVRQQLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKNLEVEKLKQKEQERKMFL---ICIFLPLYIEKAPLTISAQEDVKIVYYRALYPFEARSHDEISIQPGDIVMVDESQTGEP--GWLGGELKGKT--GWFPANYAEKI--PDNQVPTSVKPIVDTTVASKVSLRETPPTPAAPAPAESTATTNNWADFSSTYVWQVRQRSAFTP-ATVTGSSPSPVLGQGEKVEGLQAQALYPWRAKKD-------NHLNFNKNDIITVLEQQ-DMWWFGE-----VQGQK----GWFPKSYVKLI---------SGPIRKSTRYFAKAAPT------SISHFLTTSPPE----YIAMYTYESSEQGDLIFQQGDMILVTKKDGDWWTGTLGDKS--GVFPSNYVRLKDCE-------------VP----------------GTAGKTGSLGKKP--EIAQV--------------IASYTATGPE--------------QLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYV------------KLLSPGTMC----------------------------------QVIGMYDYTAQNDDELAFNKGQIINVLNKDDPDWWKGE--VNGQVGLFPSNYVKLTTDMDPSQQ-----------------------WCAD--LHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGERMPVKMIGDILTAQLPHMQPYIRFCSCQLNGAALIQQKTDEVTEFKEFVKRLAMDPRCKGMPLSSFLLKPMQRVTRYPLIIKNIIENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQ---------IIKPLGSSSTDKVFSPKSNLQYKMYKTPIFLNEVLVK--LPTDPSGDE----PIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNIVEGIELK--PCRSHGKSNPYCEVTMGSQCHITKTMQDTLNPKWNSNCQFFIKDLEQDVLCITVFERDQFSPDDFLGRTEIRVADIKKDQG-SKGPVTKCLLLHEVPTGEIVVRLDLQFCDK 1558
BLAST of Intersectin-1 vs. TrEMBL
Match: A0A2U3ZAA7 (intersectin-2 OS=Odobenus rosmarus divergens OX=9708 GN=ITSN2 PE=4 SV=1) HSP 1 Score: 718.768 bits (1854), Expect = 0.000e+0 Identity = 601/1849 (32.50%), Postives = 927/1849 (50.14%), Query Frame = 3
Query: 33 QIDLHMLNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLP--LSLNPLQTNATTMSYS-GYMPVSNTYNTMPVMHNTSHAMSNNN-----------------------PNNT------MAVPHASAFVNYXXXXXXXXXXXXXXXXNAQFGSXXXXXXXXXXSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXKKNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAILDEQMRQXXXXXXXXXXXXXXXXXXXXQEKEQARFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKMRLESEKKRLLEMEKRRLEVAIXXXXXXXXXXXXVNSKKGNL---IDALANEKE----KKRDLQSRIESTQITLETYKAG----IGSMREHRDKLIDTTDCXXXXXXXXXXXXSRCQREKXXXXXXXXXXXXXDPVLEQIKTVQALKSSHXXXXXXXXXXXXXXXQ--------RMTHRGQKLA------TNRNKVRDLEKK----VTSKLLSDIKKLQTSVCEKKNAAIAILKKQKEPKTA-VSEKNYNEFDKIGISKNPQLNSMPSNELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKL-SG-----------PPSTESTQ-------------TKIDVNQNISSGFADL-----------FNNDITPKGTLRKSSECQW-----AEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLN---LSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALYYPQNTVPVNSNGASTPEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYN---TGGMPFVFPLETSSKDKMQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMFKK 5246
Q M PN+W ITSEE+ K D QF L P G+ITG+QA++ F +S LP VL IW L+D +KDG M+ EF+IAM LIK KL+G ++P VLP + P+ + + + G MP + +++P + + +S+ PN T +++P++S+ + +++S ++ S + S ++ S N P T +W +PQ R +YR +FN D++ G+L+G +A+ L+QS L T LA+IW+LADID DG L EEF +AMHL + A++G+ LP LP L S I+ I NG + E+E Q+K +T ED+R+ANY +G ELEKRR +L EQ ++E E + KE+ E ER ++ QE+E + E+++++E +R+ E +REE+ R+ E RE + E E++R LE E+ R + +++R +EQE + +NSKK +L ++AL + + + +D++ R ++ + LE I +++ + +L D + L + + R + + L L LE+ + Q LK K ++ E++ R ++ Q+LA R+K++++E+K + K L D + ++ + EK+N L+K++E K + E+ E + K + P++ LV Y+A+Y FE R HDE++F +GDII ++ + EP GWLYG + G FP YV K+ SG PP+ + + QN+S F +L F ++P + Q A+A+ W + + N L+F +I +++ ++WW GE G+ GWFPK+YV+++ E+ + Y+++N S Y + G + +A Y Y + GDL G+ I V + +WW G + G+FPSNYVK E + S TSN I+ V T ++G+ EQ+ L GQLI I +K+ SGWW+GE+Q RG++R+ GWFP +V ++ SS +T + P +I MY+Y A N DEL+F K ++ +++K++P+WW G ++ GLFP NYV+ + S + ++ + Q PV E++RQ YI ELI+TE+ Y +DLQ V +VF + + +S LT E+ IF+NW +LI ++ AL++RK G K P+Q +GDIL + + Y+RFCS Q G A LQ+K FKE + S+ RC+ MP++S+ LKPMQRIT+Y LL+ +IL++T +NH D S ++ A+ A LC VNEGVRE+ENS+RLEW+Q H+QCE L+E+L FNS TNCLG RKL+HSG L K KS KEL GFLFNDF LL + G +F SSK QFKMYK PI LN+V++ + SS F + I V + LR + N+R WV+++ AS YI+TE KRE + R +T+G+GRL+V V+E L+ + + KS+P+CE S+G+Q + T+ +P TLNPKWN QF IK++ QDVLCLT++++D F+P+DFLGR EV V + TE+ ++ P +RL L EV GE+ VR DL +F++
Sbjct: 4 QFPTAMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSMPHSLPPVAPMATPLSSATSGTTLPPLMMPAPLVPSVSTSSLPNGTAGLTQPLSIPYSSSTLPHTSSYSLMMGGLGSGASLQKAQSLIDLGSSSSTSSTASLSGNSPK----TGIAEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPEL------------VPPSFRGGKQIDSI-NGTLPSYQKIQEEEPQKKLPVTFEDKRKANYERGNVELEKRRQVLMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQDYQNKLIYLVPEKQLLNERIKNMQLGNTPDLGIGLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKELRESYNTQQLALEQLHKIKRDKLKEIERKRSELIQKKKLED-EAIRKAKQEKENLWRESLRKEEEEKQKRLQEEKTQEKVQEEERKVEEKQCEPASALVNYKALYRFEARSHDEMSFNSGDIIQVDEKTVGEP--GWLYGSFQG--NFGWFPCNYVEKMPSGEKSVSPKKALLPPTVSVSATSTSSESLSSNQPASVTDYQNVS--FTNLTVNTSWQKKSAFTRTVSPGSISPIHGQGQVVENLKAQALCSWTAKKD-------NHLNFSKHDIITVLEQQ-ENWWFGEVHG----GR-----GWFPKSYVKIIPGSEVKR-------------EEPEALYAAINKKPTSAAYTV----GEE---YIALYSYSSVEPGDLTFTEGEEILVTQKDGEWWTG--SIGDRTGIFPSNYVKPKDQESFGSAS-------------------------------KSGTSNKKPEIAQV-----------TSAYVASGS-----------------EQLSLAPGQLILILKKNSSGWWQGELQARGKKRQKGWFPASHVKLL----------------------------------GPSSERTTPAFH---PVCQVIAMYDYGANNEDELNFSKGQLINVLNKDDPDWWQGE--INGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQ--------PV-----------------ERKRQGYIHELIQTEERYVDDLQLVVEVFQKRMVESGFLTEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTLENHIDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLF----SSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRV------YTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVQVIEATELK--ACKPNGKSNPYCEISMGSQSYTTRTLPDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQE-SKGPTTRRLLLHEVPTGEVWVRFDLQLFEQ 1653
BLAST of Intersectin-1 vs. TrEMBL
Match: A0A3Q7Q5V7 (intersectin-2 isoform X3 OS=Callorhinus ursinus OX=34884 GN=ITSN2 PE=4 SV=1) HSP 1 Score: 716.072 bits (1847), Expect = 0.000e+0 Identity = 601/1849 (32.50%), Postives = 927/1849 (50.14%), Query Frame = 3
Query: 33 QIDLHMLNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLP--LSLNPLQTNATTMSYS-GYMPVSNTYNTMPVMHNTSHAMSNNN-----------------------PNNT------MAVPHASAFVNYXXXXXXXXXXXXXXXXNAQFGSXXXXXXXXXXSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXKKNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAILDEQMRQXXXXXXXXXXXXXXXXXXXXQEKEQARFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKMRLESEKKRLLEMEKRRLEVAIXXXXXXXXXXXXVNSKKGNL---IDALANEKE----KKRDLQSRIESTQITLETYKAG----IGSMREHRDKLIDTTDCXXXXXXXXXXXXSRCQREKXXXXXXXXXXXXXDPVLEQIKTVQALKSSHXXXXXXXXXXXXXXXQ--------RMTHRGQKLA------TNRNKVRDLEKK----VTSKLLSDIKKLQTSVCEKKNAAIAILKKQKEPKTA-VSEKNYNEFDKIGISKNPQLNSMPSNELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKL-SG-----------PPSTESTQ-------------TKIDVNQNISSGFADL-----------FNNDITPKGTLRKSSECQW-----AEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLN---LSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALYYPQNTVPVNSNGASTPEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYN---TGGMPFVFPLETSSKDKMQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMFKK 5246
Q M PN+W ITSEE+ K D QF L P G+ITG+QA++ F +S LP VL IW L+D +KDG M+ EF+IAM LIK KL+G ++P VLP + P+ + + + G MP + +++P + + +S+ PN T +++P++S+ + +++S ++ S + S ++ S N P T +W +PQ R +YR +FN D++ G+L+G +A+ L+QS L T LA+IW+LADID DG L EEF +AMHL + A++G+ LP LP L S I+ I NG + E+E Q+K +T ED+R+ANY +G ELEKRR +L EQ ++E E + KE+ E ER ++ QE+E + E+++++E +R+ E +REE+ R+ E RE + E E++R LE E+ R + +++R +EQE + +NSKK +L ++AL + + + +D++ R ++ + LE I +++ + +L D + L + + R + + L L LE+ + Q LK K ++ E++ R ++ Q+LA R+K++++E+K + K L D + ++ + EK+N L+K++E K + E+ E + K + P++ LV Y+A+Y FE R HDE++F +GDII ++ + EP GWLYG + G FP YV K+ SG PP+ + + QN+S F +L F ++P + Q A+A+ W + + N L+F +I +++ ++WW GE G+ GWFPK+YV+++ E+ + Y+++N S Y + G + +A Y Y + GDL G+ I V + +WW G + G+FPSNYVK E + S TSN I+ V T ++G+ EQ+ L GQLI I +K+ SGWW+GE+Q RG++R+ GWFP +V ++ SS +T + P +I MY+Y A N DEL+F K ++ +++K++P+WW G ++ GLFP NYV+ + S + ++ + Q PV E++RQ YI ELI+TE+ Y +DLQ V +VF + + +S LT E+ IF+NW +LI ++ AL++RK G K P+Q +GDIL + + Y+RFCS Q G A LQ+K FKE + S+ RC+ MP++S+ LKPMQRIT+Y LL+ +IL++T +NH D S ++ A+ A LC VNEGVRE+ENS+RLEW+Q H+QCE L+E+L FNS TNCLG RKL+HSG L K KS KEL GFLFNDF LL + G +F SSK QFKMYK PI LN+V++ + SS F + I V + LR + N+R WV+++ AS YI+TE KRE + R +T+G+GRL+V V+E L+ + + KS+P+CE S+G+Q + T+ +P TLNPKWN QF IK++ QDVLCLT++++D F+P+DFLGR EV V + TE+ ++ P +RL L EV GE+ VR DL +F++
Sbjct: 4 QFPTAMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSMPHSLPPVAPMATPLSSATSGTTLPPLMMPAPLVPSVSTSSLPNGTAGLIQPLSIPYSSSTLPHTSSYSLMMGGLGSGASLQKAQSLIDLGSSSSTSSTASLSGNSPK----TGIAEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPEL------------VPPSFRGGKQIDSI-NGTLPSYQKIQEEEPQKKLPVTFEDKRKANYERGNVELEKRRQVLMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQDYKNKLIYLVPEKQLLNERIKNMQLGNTPDLGIGLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKELRESYNTQQLALEQLHKIKRDKLKEIERKRSELIQKKKLED-EAIRKAKQEKENLWRESLRKEEEEKQKRLQEEKTQEKVQEEEQKVEEKQCEPASALVNYKALYRFEARSHDEMSFNSGDIIQVDEKTIGEP--GWLYGSFQG--NFGWFPCNYVEKMPSGEKSVSPKKALLPPTVSVSATSTSSESLSSNQPASVTDYQNVS--FTNLTVNTSWQKKSAFTRTVSPGSISPIHGQGQVVENLKAQALCSWTAKKD-------NHLNFSKHDIITVLEQQ-ENWWFGEVHG----GR-----GWFPKSYVKIIPGSEVKR-------------EEPEALYAAINKKPTSAAYTV----GEE---YIALYSYSSVEPGDLTFTEGEEILVTQKDGEWWTG--SIGDRTGIFPSNYVKPKDQESFGSAS-------------------------------KSGTSNKKPEIAQV-----------TSAYVASGS-----------------EQLSLAPGQLILILKKNSSGWWQGELQARGKKRQKGWFPASHVKLL----------------------------------GPSSERTTPAFH---PVCQVIAMYDYGANNEDELNFSKGQLINVLNKDDPDWWQGE--INGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQ--------PV-----------------ERKRQGYIHELIQTEERYVDDLQLVVEVFQKRMVESGFLTEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTLENHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLF----SSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRV------YTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVQVIEATELK--ACKPNGKSNPYCEISMGSQSYTTRTLPDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQE-SKGPTTRRLLLHEVPTGEVWVRFDLQLFEQ 1653
BLAST of Intersectin-1 vs. TrEMBL
Match: A0A3Q7NPQ8 (intersectin-2 isoform X2 OS=Callorhinus ursinus OX=34884 GN=ITSN2 PE=4 SV=1) HSP 1 Score: 716.072 bits (1847), Expect = 0.000e+0 Identity = 601/1842 (32.63%), Postives = 927/1842 (50.33%), Query Frame = 3
Query: 54 NNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLP--LSLNPLQTNATTMSYS-GYMPVSNTYNTMPVMHNTSHAMSNNN-----------------------PNNT------MAVPHASAFVNYXXXXXXXXXXXXXXXXNAQFGSXXXXXXXXXXSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXKKNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAILDEQMRQXXXXXXXXXXXXXXXXXXXXQEKEQARFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKMRLESEKKRLLEMEKRRLEVAIXXXXXXXXXXXXVNSKKGNL---IDALANEKE----KKRDLQSRIESTQITLETYKAG----IGSMREHRDKLIDTTDCXXXXXXXXXXXXSRCQREKXXXXXXXXXXXXXDPVLEQIKTVQALKSSHXXXXXXXXXXXXXXXQ--------RMTHRGQKLA------TNRNKVRDLEKK----VTSKLLSDIKKLQTSVCEKKNAAIAILKKQKEPKTA-VSEKNYNEFDKIGISKNPQLNSMPSNELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKL-SG-----------PPSTESTQ-------------TKIDVNQNISSGFADL-----------FNNDITPKGTLRKSSECQW-----AEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLN---LSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALYYPQNTVPVNSNGASTPEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYN---TGGMPFVFPLETSSKDKMQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMFKK 5246
PN+W ITSEE+ K D QF L P G+ITG+QA++ F +S LP VL IW L+D +KDG M+ EF+IAM LIK KL+G ++P VLP + P+ + + + G MP + +++P + + +S+ PN T +++P++S+ + +++S ++ S + S ++ S N P T +W +PQ R +YR +FN D++ G+L+G +A+ L+QS L T LA+IW+LADID DG L EEF +AMHL + A++G+ LP LP L S I+ I NG + E+E Q+K +T ED+R+ANY +G ELEKRR +L EQ ++E E + KE+ E ER ++ QE+E + E+++++E +R+ E +REE+ R+ E RE + E E++R LE E+ R + +++R +EQE + +NSKK +L ++AL + + + +D++ R ++ + LE I +++ + +L D + L + + R + + L L LE+ + Q LK K ++ E++ R ++ Q+LA R+K++++E+K + K L D + ++ + EK+N L+K++E K + E+ E + K + P++ LV Y+A+Y FE R HDE++F +GDII ++ + EP GWLYG + G FP YV K+ SG PP+ + + QN+S F +L F ++P + Q A+A+ W + + N L+F +I +++ ++WW GE G+ GWFPK+YV+++ P S E+ + Y+++N S Y + G + +A Y Y + GDL G+ I V + +WW G + G+FPSNYVK E + S TSN I+ V T ++G+ EQ+ L GQLI I +K+ SGWW+GE+Q RG++R+ GWFP +V ++ SS +T + P +I MY+Y A N DEL+F K ++ +++K++P+WW G ++ GLFP NYV+ + S + ++ + Q PV E++RQ YI ELI+TE+ Y +DLQ V +VF + + +S LT E+ IF+NW +LI ++ AL++RK G K P+Q +GDIL + + Y+RFCS Q G A LQ+K FKE + S+ RC+ MP++S+ LKPMQRIT+Y LL+ +IL++T +NH D S ++ A+ A LC VNEGVRE+ENS+RLEW+Q H+QCE L+E+L FNS TNCLG RKL+HSG L K KS KEL GFLFNDF LL + G +F SSK QFKMYK PI LN+V++ + SS F + I V + LR + N+R WV+++ AS YI+TE KRE + R +T+G+GRL+V V+E L+ + + KS+P+CE S+G+Q + T+ +P TLNPKWN QF IK++ QDVLCLT++++D F+P+DFLGR EV V + TE+ ++ P +RL L EV GE+ VR DL +F++
Sbjct: 45 GGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSMPHSLPPVAPMATPLSSATSGTTLPPLMMPAPLVPSVSTSSLPNGTAGLIQPLSIPYSSSTLPHTSSYSLMMGGLGSGASLQKAQSLIDLGSSSSTSSTASLSGNSPK----TGIAEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPEL------------VPPSFRGGKQIDSI-NGTLPSYQKIQEEEPQKKLPVTFEDKRKANYERGNVELEKRRQVLMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERIRRQELLNQRNREQEEIVRLNSKKKSLHLELEALNGKHQQISGRLQDVRLRKQTQKTELEVLDKQCDLEIMEIKQLQQELQDYKNKLIYLVPEKQLLNERIKNMQLGNTPDLGIGLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKELRESYNTQQLALEQLHKIKRDKLKEIERKRSELIQKKKLED-EAIRKAKQEKENLWRESLRKEEEEKQKRLQEEKTQEKVQEEEQKVEEKQCEPASALVNYKALYRFEARSHDEMSFNSGDIIQVDEKTIGEP--GWLYGSFQG--NFGWFPCNYVEKMPSGEKSVSPKKALLPPTVSVSATSTSSESLSSNQPASVTDYQNVS--FTNLTVNTSWQKKSAFTRTVSPGSISPIHGQGQVVENLKAQALCSWTAKKD-------NHLNFSKHDIITVLEQQ-ENWWFGEVHG----GR-----GWFPKSYVKII-------PGSEV------KREEPEALYAAINKKPTSAAYTV----GEE---YIALYSYSSVEPGDLTFTEGEEILVTQKDGEWWTG--SIGDRTGIFPSNYVKPKDQESFGSAS-------------------------------KSGTSNKKPEIAQV-----------TSAYVASGS-----------------EQLSLAPGQLILILKKNSSGWWQGELQARGKKRQKGWFPASHVKLL----------------------------------GPSSERTTPAFH---PVCQVIAMYDYGANNEDELNFSKGQLINVLNKDDPDWWQGE--INGVTGLFPSNYVKMTTDSDPSQQWCADLQSLDTMQ--------PV-----------------ERKRQGYIHELIQTEERYVDDLQLVVEVFQKRMVESGFLTEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTLENHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLF----SSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRV------YTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVQVIEATELK--ACKPNGKSNPYCEISMGSQSYTTRTLPDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLGRTEVPVAKIRTEQE-SKGPTTRRLLLHEVPTGEVWVRFDLQLFEQ 1687
BLAST of Intersectin-1 vs. Ensembl Cavefish
Match: itsn1 (intersectin 1 (SH3 domain protein) [Source:ZFIN;Acc:ZDB-GENE-030616-226]) HSP 1 Score: 554.288 bits (1427), Expect = 1.055e-167 Identity = 356/1039 (34.26%), Postives = 532/1039 (51.20%), Query Frame = 3
Query: 2289 NISSGFADLFNNDITPKGTLRKSSECQWAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQN--------------ANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQK---LLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTIS----DV--------------ENNSALYYPQNTV-PVNSNG----------ASTPEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYNTGGMPFVFPLETSSKDK-------MQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMF 5240
NI S F + + G + + A+Y ++ S+ ++++ + G ++ + D+ TGE L K K GWFP Y + E A P P+ G S ++ LS +K L A YP++A LN D+I VL+Q+ WW + +G FP +YVK+++ N + A ++ + ++N++ I++ + SN ++ DV + +P N V P +S+G PEIAQV+ + AEQ+ L GQLI IR+K+P GWW+GE+Q RG++R+IGWFP Y ++L +I MY+Y+AQN DEL F K I+ ++ + +P+WW G L+ GLFP NYV+ + S + W D + +LT E++RQ YI ELI TE+ Y DLQ V + F +P+ +S LLT E+ IF+NW +LI + AL++RK ++G + P++ +GDIL + P+ Y+RFCS Q G +Q+K P FK+ + + RC+ MP++S+ LKPMQR+T+Y L+++NIL++T ++HPD S + +A+ A LC VNEGVRE+ENS+RLEW+Q H+QCE LSE+L FNS TNCLG RK +HSG L K KS KEL GFLFNDF LL + PL +S+ DK +Q++MYK PI LN+V++ PT + + IF + + +RA S N+R WV+++ AS +I TE KRE + +R R G+GRL+V +VEGI L+ KS+P+CE ++G+Q H TK + TLNPKWN+ QF IK+++QDVLC+TV+ +D F+P+DFLGR E+ + ++ ++ ++ PI KRL L EV GE+VVRLDL +F
Sbjct: 630 NIISCIVVCFTDKVPMSGFNQDKVRVVYYRALYPFEARSH-------DEITIQPGDIVMV-----DESQTGEPGWLGGEIKGKT--GWFPANYAEKIPESEVPLNLRASATSGSPAQPPQPSLSVPSGGQGRQ-RSAFTPATLSGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWW--FGEVQGQRGWFPKSYVKLIS----GNKSISALAHEHSYMQIYR--LCMVNSTATPYIAMYTYESNEQGDLTFQQGDVITVLKKEGDWWTGTVSGKTGVFPSNYVKPKDSDGLGPAGKTGSLGKKPEIAQVIAPYTATGAEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANY----------------------------------------------VKLLSPSTMCQVIGMYDYAAQNDDELPFGKGQIINVLSREDPDWWKGE--LNGSVGLFPSNYVKLTTDTDPSQQ-----------------------WCAD--LHLLDMLTPMERKRQGYIHELIITEENYVNDLQLVTETFQKPLLESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGERMPVKMIGDILTAQLPHMQPYIRFCSCQLNGATLIQQKTDEVPDFKDFVKRLAMDPRCKGMPLSSFLLKPMQRVTRYPLIIKNILENTPESHPDHSHLRQALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQ---------VIKPLGSSATDKVFSAKSHLQYRMYKTPIFLNEVLVK--LPTDPSGDE----PIFHISHIDRVYTIRAESINERTAWVQKIKAASELFIETEKKKREKAYLVRSQRATGIGRLMVNIVEGIELK--PCRSHGKSNPYCEVTMGSQCHITKTLQDTLNPKWNSNCQFFIKDLEQDVLCVTVFERDQFSPDDFLGRTEIRLADIKKDQG-SKGPITKRLLLHEVPTGEIVVRLDLQLF 1554 HSP 2 Score: 124.79 bits (312), Expect = 5.353e-28 Identity = 122/446 (27.35%), Postives = 186/446 (41.70%), Query Frame = 3
Query: 2052 LVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRK---------LSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSS-----------ECQWAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALYYPQNT-----------VPVNSNGASTPEIAQVVIEFNPLSAE---QIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYV 3287
+VYY A+Y FE R HDEIT + GDI++++ EP GWL G + + G FP Y K L ++ S + ++ SG + TP TL SS E A+A+Y W+ + + N L+F +I +++ D WW GE G+ GWFPK+YV+L+ SG S+ LA ++S + + Y L + S +A Y Y+++ GDL Q GD+I VL ++ DWW G + G+FPSNYVK S+ L P E+++ I + ++A +T P I + N ++ P N +P VI +A+ ++P GQ+I + + WWKGE+ FP YV
Sbjct: 655 VVYYRALYPFEARSHDEITIQPGDIVMVDESQTGEP--GWLGGEIKGKT--GWFPANYAEKIPESEVPLNLRASATSGSPAQPPQPSLSVPSGGQGRQRSAFTP-ATLSGSSPSPVLGQGEKVEGLQAQALYPWRAKKD-------NHLNFNKNDVITVLEQQ-DMWWFGE-----VQGQR----GWFPKSYVKLI---------SGN-----KSISALAHEHSYMQI---YRLCMVN-STATPYIAMYTYESNEQGDLTFQQGDVITVLKKEGDWWTG--TVSGKTGVFPSNYVKPKDSDGLGPAGKTGSLGKKP--EIAQVI------APYTATGAEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPSTMCQVIGMYDYAAQNDDELPFGKGQIINVLSREDPDWWKGELNGSVGL-----FPSNYV 1045 HSP 3 Score: 116.316 bits (290), Expect = 2.011e-25 Identity = 77/168 (45.83%), Postives = 104/168 (61.90%), Query Frame = 3
Query: 654 DWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXKKNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAILDEQMRQ 1157
DW +PQS R +YR FN D+ G LTG +A+ IL+QS LP LA+IW+L+DIDQDG LT EEF +AMHL++ A SG LP LP LLPP+ + + TS+ +P V + + EKE+ +K +T ED++R N+ +G ELEKRR L EQ R+
Sbjct: 214 DWAVPQSSRLKYRQLFNSHDKMMSGHLTGPQARTILMQSSLPQAQLATIWNLSDIDQDGKLTAEEFILAMHLIDMAMSGLPLPPLLPPDLLPPTFRSDGVSVTSL--------HPADQRVQEESAEEEEKEADKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRK 373 HSP 4 Score: 69.707 bits (169), Expect = 3.159e-11 Identity = 32/59 (54.24%), Postives = 43/59 (72.88%), Query Frame = 3
Query: 126 PVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGY 302
P + F TG+QAK+ F +S LP VL +IW LAD + DG M+ +EF+IAM LIK KL+G+
Sbjct: 34 PNHSFSTGDQAKNFFLQSGLPAPVLAQIWALADLNNDGKMDMHEFSIAMKLIKLKLQGH 92 HSP 5 Score: 58.9214 bits (141), Expect = 6.005e-8 Identity = 28/81 (34.57%), Postives = 47/81 (58.02%), Query Frame = 3
Query: 60 PNLWFITSEEKVKFDVQFQCLNPV-NGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEG 299
P W + ++K+ F + + +G +TG QA+++ +S+LP L IW L+D D+DG + + EF +AMHLI + G
Sbjct: 212 PIDWAVPQSSRLKYRQLFNSHDKMMSGHLTGPQARTILMQSSLPQAQLATIWNLSDIDQDGKLTAEEFILAMHLIDMAMSG 292 HSP 6 Score: 58.5362 bits (140), Expect = 8.848e-8 Identity = 32/90 (35.56%), Postives = 45/90 (50.00%), Query Frame = 3
Query: 621 KPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSG 890
+PP H + D W S + + + F TG +AK +QSGLP LA IW+LAD++ DG + EF IAM L++ G
Sbjct: 5 QPPAHNGGTLDHWTTRYSNNLCF---YGMTNLPNHSFSTGDQAKNFFLQSGLPAPVLAQIWALADLNNDGKMDMHEFSIAMKLIKLKLQG 91
BLAST of Intersectin-1 vs. Ensembl Cavefish
Match: ENSAMXT00000039253.1 (pep primary_assembly:Astyanax_mexicanus-2.0:3:25018794:25036691:1 gene:ENSAMXG00000029292.1 transcript:ENSAMXT00000039253.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 449.899 bits (1156), Expect = 7.162e-141 Identity = 246/597 (41.21%), Postives = 359/597 (60.13%), Query Frame = 3
Query: 3465 MYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYNTGGMPFVFPLETSSKDKMQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMFKKN 5249
MY+Y A + DE+SF K ++ + +K++ +WW G L+ GLFP NY++ + S + W D ++ +++ +E E++RQ YI ELI TE Y +DL+ V +VF++P+ +S LT E+ IF+NW +LI + AL++RK AG + P+Q +GDIL S + Y+RFCS Q A LQ+K + P FK + +N RC+ MP++S+ LKPMQRIT+Y LL+ NIL++T H D++++ EA+ A LC VNEGVRE+ENS+RLEW+Q H+QCE + E L FNS TNCLG RKL+HSG + K KS KEL FLF+DF LL Y S K +KMYK P+ LN+V++ S SS + F + I V + L+A S ++R WV+R+ AS +I TE LKRE + R +T+G+GRLLVTV E + L+ + KS+P+CE S+G Q + ++ T+NPKWN QF IK++ QDVLC+T++ +D F+P+DFLGR EV V + ++ + P+ +RL L EV GE+ VRLDL ++++
Sbjct: 1 MYDYKAASEDEMSFLKGQLINVFNKDDSDWWKGE--LNGVTGLFPTNYIKMTTESDPSQQ-----------------------WCAD-LLSLDSMSSE-ERKRQGYIHELIHTEHTYLQDLELVLEVFYKPMTESGRLTEAEMGVIFVNWRELIMCNTKLLKALRVRKKTAGERMPVQMIGDILSSELAHMQAYIRFCSCQLNAAALLQQKTDASPDFKLFLKKIATNYRCKGMPLSSFLLKPMQRITRYPLLIRNILENTPAGHADQANLREALDRAEELCSQVNEGVREKENSDRLEWIQNHVQCEGVIEHLVFNSLTNCLGPRKLLHSGRVWKTKSSKELWAFLFSDFLLLT---YTAKQFSSSTDKLFSPKSNTMYKMYKTPVFLNEVLVKMPSDPSSDDPIFHISHIDRV------YTLKADSISERTTWVQRIKAASEHFIETEKLKREKAYQARSQKTSGIGRLLVTVQEAVELK--PCKPNGKSNPYCELSMGAQCYTSRPQNDTVNPKWNFNCQFFIKDLYQDVLCITIFERDQFSPDDFLGRTEVPVATIKKDQE-GKGPLTRRLLLHEVPTGEVRVRLDLQLYEQG 558
BLAST of Intersectin-1 vs. Ensembl Cavefish
Match: ENSAMXT00000007791.2 (pep primary_assembly:Astyanax_mexicanus-2.0:9:9948415:9965257:-1 gene:ENSAMXG00000007547.2 transcript:ENSAMXT00000007791.2 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 421.779 bits (1083), Expect = 3.012e-131 Identity = 227/501 (45.31%), Postives = 314/501 (62.67%), Query Frame = 3
Query: 3750 LTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLL--ATPCYNTGGMPFVFPLETSSKDKMQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMF 5240
++ EK+RQ YI ELIETE+ Y EDLQ V +VF +P+ +S LT E+ IF+NW +LI + AL++RK G K P+Q VGDIL + + Y+RFCS Q A LQ+K P FK+ + ++ RC+ MP++S+ LKPMQRIT+Y L ++NIL+ T D+H D S + EA+ A LC VNEGVRE+ENS+RLEW+Q H+QCE ++E L FNS TNCLG RKL+HSG L K KS KEL FLF DF L A + + G +F S K Q+++YK P+ LN+V++ S SS F + I V + L+ + N+R WV+++ AS +I TE KRE + R + +G+GRLLVT+ E L+ SS KS+P+CE ++G Q + ++ + T+NPKWN Q QF IK++ QDVLC+T+Y ++ FAP+DFLGR EV V + E + P +RL L EV GE+ VRLDL +F
Sbjct: 22 MSPQEKKRQGYIHELIETEERYMEDLQLVLEVFHKPMSESGRLTEAEMNMIFVNWKELIMCNTKLVKALRVRKKTGGEKMPVQMVGDILAAELSHMQAYIRFCSCQLNAAALLQQKTDQEPDFKDFLKKIATDYRCKGMPLSSFLLKPMQRITRYPLHIKNILESTPDSHVDHSQLREALEKAEELCSQVNEGVREKENSDRLEWIQNHVQCEGITESLVFNSLTNCLGPRKLLHSGKLFKTKSNKELYAFLFTDFLLFTQAVKQFTSSGADKLF----SPKSSTQYRIYKSPVFLNEVLVKMPSDPSSDEPVFHISHIDRV------YTLKTENINERTAWVQKIKAASEEFIETEKKKREKAYQARSMKASGIGRLLVTIQEATELK--SSKPSGKSNPYCEVTMGAQCYTSRTLNDTVNPKWNFQCQFHIKDLYQDVLCITIYEREQFAPDDFLGRTEVPVATIKKELE-NKGPATRRLLLHEVPTGEVWVRLDLQLF 509
BLAST of Intersectin-1 vs. Ensembl Cavefish
Match: ENSAMXT00000053298.1 (pep primary_assembly:Astyanax_mexicanus-2.0:3:25025725:25036691:1 gene:ENSAMXG00000029292.1 transcript:ENSAMXT00000053298.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 416.772 bits (1070), Expect = 9.792e-129 Identity = 222/502 (44.22%), Postives = 317/502 (63.15%), Query Frame = 3
Query: 3750 LTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYNTGGMPFVFPLETSSKDKMQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMFKKN 5249
++ +E++RQ YI ELI TE Y +DL+ V +VF++P+ +S LT E+ IF+NW +LI + AL++RK AG + P+Q +GDIL S + Y+RFCS Q A LQ+K + P FK + +N RC+ MP++S+ LKPMQRIT+Y LL+ NIL++T H D++++ EA+ A LC VNEGVRE+ENS+RLEW+Q H+QCE + E L FNS TNCLG RKL+HSG + K KS KEL FLF+DF LL Y S K +KMYK P+ LN+V++ S SS + F + I V + L+A S ++R WV+R+ AS +I TE LKRE + R +T+G+GRLLVTV E + L+ + KS+P+CE S+G Q + ++ T+NPKWN QF IK++ QDVLC+T++ +D F+P+DFLGR EV V + ++ + P+ +RL L EV GE+ VRLDL ++++
Sbjct: 82 MSSEERKRQGYIHELIHTEHTYLQDLELVLEVFYKPMTESGRLTEAEMGVIFVNWRELIMCNTKLLKALRVRKKTAGERMPVQMIGDILSSELAHMQAYIRFCSCQLNAAALLQQKTDASPDFKLFLKKIATNYRCKGMPLSSFLLKPMQRITRYPLLIRNILENTPAGHADQANLREALDRAEELCSQVNEGVREKENSDRLEWIQNHVQCEGVIEHLVFNSLTNCLGPRKLLHSGRVWKTKSSKELWAFLFSDFLLLT---YTAKQFSSSTDKLFSPKSNTMYKMYKTPVFLNEVLVKMPSDPSSDDPIFHISHIDRV------YTLKADSISERTTWVQRIKAASEHFIETEKLKREKAYQARSQKTSGIGRLLVTVQEAVELK--PCKPNGKSNPYCELSMGAQCYTSRPQNDTVNPKWNFNCQFFIKDLYQDVLCITIFERDQFSPDDFLGRTEVPVATIKKDQE-GKGPLTRRLLLHEVPTGEVRVRLDLQLYEQG 571
BLAST of Intersectin-1 vs. Ensembl Cavefish
Match: arhgef11 (Rho guanine nucleotide exchange factor (GEF) 11 [Source:ZFIN;Acc:ZDB-GENE-070604-1]) HSP 1 Score: 124.79 bits (312), Expect = 5.800e-28 Identity = 82/250 (32.80%), Postives = 133/250 (53.20%), Query Frame = 3
Query: 3750 LTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPI-QSVGDILLSNFPN-----FIVYV-RFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEK---LYFNSNTNCLGHRKLIHSGTLS 4469
L+ E RQ I EL TE + L+ + QVFF+ K N+LT +EL IF N S + N ++K +++ PI QS+GD++L+ F + F V CS Q+Q + ++ KQ+ P+F L + CE++ C+++ + + MQR+TKY LL++NI+K+TE PD ++ A A R + ++VNE VRE E+ +RL Q + L + + L +++IH G L+
Sbjct: 716 LSSREIDRQAVIYELFTTEASHLRTLRVLDQVFFQ--KMRNVLTAEELSCIFPNLSQVYELHANLLESMKKQRE-----SPIVQSIGDVMLARFEDAAGEEFQEQVSHLCSQQSQALELIKSKQRKDPRFAHLIQECEASPLCRRLQLKDLLVSEMQRLTKYPLLLDNIIKYTEAVSPDLQQLQRAQACCRGILQTVNEVVRETEHRQRLSQYQRRLDSSPLERQANPVAAQFKNLDLTTKRMIHEGPLT 958
BLAST of Intersectin-1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007679.1 (pep scaffold:Pmarinus_7.0:GL483514:540:14719:1 gene:ENSPMAG00000006927.1 transcript:ENSPMAT00000007679.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 130.183 bits (326), Expect = 4.070e-31 Identity = 107/320 (33.44%), Postives = 159/320 (49.69%), Query Frame = 3
Query: 216 LADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLPLSL------NPLQTNATTMSYSGYMPVSNTYNTMPVMHNTSHAMSNNNPNNTMAVPHASAFVNYXXXXXXXXXXXXXXXXNAQFGSXXXXXXXXXXSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXKKNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAILDEQMRQ 1157
LAD + DG M++ EF+IAM LIK +L G+ +P VLP ++ PL A + G + +T P+ +++ P +T+ +P A + + GS P + K T DW +PQS R +YR FN D+ FLTG +A+ IL+QS L LA+IW L+DIDQDG LT EEF +AMHL++ A SG+QLP L L+PPS ++ + S + ++ + +E+E ++K +T ED++R N+ KG ELE+RRA L E R+
Sbjct: 8 LADLNGDGRMDAQEFSIAMKLIKMRLHGHALPAVLPPAILQRSPFGPLVGGAPLLGMGGSL--GSTLVGGPLGAVAPSSLTP--PLSTVGIP-APMANGVQGLMQPFPTTGHSCGATLPKGSTFGRPGAGVGPGIGPIQMQKALSAEATMAADWAVPQSSRLKYRQLFNSHDKTMSSFLTGPQARNILMQSHLTQPQLAAIWGLSDIDQDGKLTAEEFILAMHLIDMALSGQQLPPALTADLMPPSFRRSRTGSSLSGGSSSSGVSVSLLEQKPPEEAAAEEEQEKKLPVTFEDKKRENFEKGNQELERRRAALAETQRR 322 HSP 2 Score: 67.0106 bits (162), Expect = 2.796e-11 Identity = 31/91 (34.07%), Postives = 51/91 (56.04%), Query Frame = 3
Query: 69 WFITSEEKVKFDVQFQCLNP-VNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLPLSLNP 338
W + ++K+ F + ++ F+TG QA+++ +S+L L IW L+D D+DG + + EF +AMHLI L G ++P L L P
Sbjct: 156 WAVPQSSRLKYRQLFNSHDKTMSSFLTGPQARNILMQSHLTQPQLAAIWGLSDIDQDGKLTAEEFILAMHLIDMALSGQQLPPALTADLMP 246
BLAST of Intersectin-1 vs. Ensembl Sea Lamprey
Match: ARHGEF11 (Rho guanine nucleotide exchange factor 11 [Source:HGNC Symbol;Acc:HGNC:14580]) HSP 1 Score: 122.094 bits (305), Expect = 6.062e-28 Identity = 79/241 (32.78%), Postives = 137/241 (56.85%), Query Frame = 3
Query: 3789 ELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMAL-KIRKDLAGVKQPIQSVGDILLSNFPNFIVYV------RFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDN---HPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNC----LGHRKLIHSGTLS 4469
EL TEK + ++L+ +++VFF + + +LT +E IF+N +++P + A+ KIR+ Q + +GD++L+ F V +FCS+Q+Q + ++ KQ+ P+F++L ES +C+++ + + L MQR+TKY LL++NILK+T+ HPD + +A R + VNE VRE EN++RLE Q + +L E+ Y T+ L H+K+++ G L+
Sbjct: 721 ELFHTEKKHVQNLKVLYEVFFS--RLAPVLTNNETYQIFINLDEILPIHESLNEAMRKIREK----SQVVAEIGDLMLARFDGEAGEVLKAATAKFCSLQSQALDIVRSKQKKDPRFQQLMMEAESKPKCRRLQLKDFMLTEMQRLTKYPLLLQNILKYTQVRTVEHPDHKKLHKAGECCRRILNDVNETVRETENAQRLEDYQRRLDLSSL-ERSYNPLVTDFKNLDLTHKKMLYEGPLT 954
BLAST of Intersectin-1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009021.1 (pep scaffold:Pmarinus_7.0:GL482604:596:7954:-1 gene:ENSPMAG00000008160.1 transcript:ENSPMAT00000009021.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 97.8265 bits (242), Expect = 4.437e-22 Identity = 74/296 (25.00%), Postives = 131/296 (44.26%), Query Frame = 3
Query: 3468 YNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFI-VYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSE 4352
+++ N DELSF K ++ + + WW G L + G FP NYVR L+ GN + + S ++ G +N +L + ++ETE+ Y+ +LQ + F P+ S+ L+ ++ ++ N D+ AL+ D V + Q+VG +S +Y+ +C + LQ+ + +F E L + +T+ KP R+ KY L++ + +H ED HPDR+D+ A+ A ++L E +R+R+ E
Sbjct: 16 FDFQRNNEDELSFSKGDVITVTRPEDGGWWEG--TLGGRSGWFPSNYVRELK-GNERPTSPKSKSSGKSVDSPISNKG-----------YYNVVL-----------QNIVETEREYWRELQHLLGTFLRPLHNSDKLSATDVSTLMGNLEDVCAFQQGLMQALE---DCTKVPESQQTVGGCFMSLMNQMHGLYLAYCCNHPWAVRTLQEHSEVLGEFMESKGVSSPGL----LMLTTSLSKPFMRLDKYPTLLKELERHMEDIHPDRNDIHGAMTAFKTLATQCRE-MRKRKELE 278
BLAST of Intersectin-1 vs. Ensembl Sea Lamprey
Match: net1 (neuroepithelial cell transforming 1 [Source:ZFIN;Acc:ZDB-GENE-041121-18]) HSP 1 Score: 98.9821 bits (245), Expect = 6.212e-22 Identity = 77/302 (25.50%), Postives = 136/302 (45.03%), Query Frame = 3
Query: 3750 LTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENS---ERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYNTGGMPFVFPLETSSKDKMQFKMYKKPIILNQVMI 4646
+ E +RQ I EL + E+ EDL+ + + +P+ K +++T EL IF LIP + L+ + G + VG IL+ P Y +C+ Q A L +K+Q P+ ++ + C + +K+ + S+ P R+ KY LL+ IL+HT + HPD+ +EEA++ + + VN E E ++L++L E + + L S T +H + G +L LF D +L+ P + D +++Y++PI L + +
Sbjct: 105 FSRSEIKRQEAIFELAQGEQDLIEDLKLAKKAYRDPMLKLSIMTAVELAQIFGALDSLIPLHEDLLTGLQKARKPNGA---TERVGHILVEWLPCLNAYTCYCANQVAAKALLDQKKQD-PRVQDFLQRCLESPFSRKLELWSFLDVPRSRLVKYPLLLREILRHTPNEHPDQQCLEEAISIIQGVVADVNTRTGESECCYFRDKLDYLDERQR-----DPLIATSRT--------LHLHGELRNNRGTKLFVLLFGDVLVLSRPA-------------SRGNDGCCYQVYRQPIPLRDLRL 376
BLAST of Intersectin-1 vs. Ensembl Sea Lamprey
Match: ehd1a (EH-domain containing 1a [Source:ZFIN;Acc:ZDB-GENE-040426-2518]) HSP 1 Score: 98.9821 bits (245), Expect = 6.587e-22 Identity = 46/89 (51.69%), Postives = 63/89 (70.79%), Query Frame = 3
Query: 72 FITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLPLSLNP 338
++ S++K +D F L+P++G I+G AK KS LP VLGRIW LAD DKDG ++ +EFA+A HLIK KLEG+E+P+ LP+ L P
Sbjct: 305 WVVSKDKPAYDEIFYTLSPIDGKISGASAKREMVKSKLPNTVLGRIWKLADVDKDGMLDDDEFALANHLIKVKLEGHELPEELPMHLLP 393 HSP 2 Score: 58.151 bits (139), Expect = 1.074e-8 Identity = 32/79 (40.51%), Postives = 45/79 (56.96%), Query Frame = 3
Query: 654 DWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSG 890
DWV+ + +P Y F G ++G AK +V+S LPNT L IW LAD+D+DG L +EF +A HL++ G
Sbjct: 304 DWVVSKD-KPAYDEIFYTLSPID-GKISGASAKREMVKSKLPNTVLGRIWKLADVDKDGMLDDDEFALANHLIKVKLEG 380
BLAST of Intersectin-1 vs. Ensembl Yeast
Match: CDC24 (Guanine nucleotide exchange factor (GEF) for Cdc42p; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress; thermosensitivity of the cdc24-4 mutant in the presence of sorbitol is functionally complemented by human CDC42 [Source:SGD;Acc:S000000039]) HSP 1 Score: 71.2478 bits (173), Expect = 1.081e-12 Identity = 66/254 (25.98%), Postives = 120/254 (47.24%), Query Frame = 3
Query: 3783 IRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHM------QCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLL 4526
I+E + TE+ Y DL E+ + + + SNL+T +EL +F N D I F ++L+I A V+ Q +G + + + F +Y + QN I L S K + + + K+ + S+ KP+QR+ +Y LLV+ +L + D++ + ++E A+ ++++ S+NE R EN + ++ L + + E LYF+ K+ S T S + +E +LF +L
Sbjct: 283 IKEFVATERKYVHDL-EILDKYRQQLLDSNLITSEELYMLFPNLGDAIDFQRRFLISLEIN---ALVEPSKQRIGALFMHSKHFFKLYEPWSIGQNAAIEFL-----SSTLHKMRVDESQRFIINNKLELQSFLYKPVQRLCRYPLLVKELLAESSDDN-NTKELEAALDISKNIARSINENQRRTENHQVVKKLYGRVVNWKGYRISKFGELLYFD---------KVFISTTNSSSEPEREFEVYLFEKIIIL 517
BLAST of Intersectin-1 vs. Ensembl Yeast
Match: ROM2 (Guanine nucleotide exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p; ROM2 has a paralog, ROM1, that arose from the whole genome duplication [Source:SGD;Acc:S000004363]) HSP 1 Score: 66.2402 bits (160), Expect = 4.796e-11 Identity = 65/269 (24.16%), Postives = 128/269 (47.58%), Query Frame = 3
Query: 3750 LTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDE----LKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKEL-CETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHT--EDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSK---YKSGKELVGFLFNDFFLL 4526
L + E +RQ I E+ TEK + + L+ F + + ++N+++ D +K +F + +D+ + F AL R+ + V ++ +GDI+L P F +V + + + ++ ++ P F + S+LR + S+ + + R +Y LLV+ I+K T E + D D+ +A+ A R + +++ ++ ++ L++ + +N L N RK+ H G LS+ KS +VG + F+LL
Sbjct: 653 LPKKEIKRQEAIYEVYITEKNFVKSLEITRDTFMKTLAETNIISADIRKNFIKHVFAHINDIYSVNRRFLKALTDRQRSSPV---VRGIGDIVLRFIPFFEPFVSYVASRPYAKYLIETQRSVNPYFARFDDDMMSSSLR---HGIDSFLSQGVSRPGRYMLLVKEIMKSTDPEKDKSDYEDLSKAMDALRDFMKRIDQASGAAQDRHDVKLLKQKILFKNEYVNLGLNDE-----RRKIKHEGILSRKELSKSDGTVVGDI--QFYLL 908
BLAST of Intersectin-1 vs. Ensembl Yeast
Match: PAN1 (Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p; associates with actin patches on cell cortex; promotes protein-protein interactions essential for endocytosis; binds to and activates Arp2/3 complex in vitro; phosphorylation of Thr-1225 is regulated by MAPK Hog1p in response to osmotic stress; previously thought to be a subunit of poly(A) ribonuclease [Source:SGD;Acc:S000001445]) HSP 1 Score: 63.929 bits (154), Expect = 2.389e-10 Identity = 38/102 (37.25%), Postives = 56/102 (54.90%), Query Frame = 3
Query: 45 HMLNNPNLWFITSEEKVKFDVQFQCLNPVN-GFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLPLSLNPLQT 347
++++N + I++EEK F F+ + N G + A +F KS L L +IW L D + G +N EFA+ MHL+ KL G +P+VLP SL P T
Sbjct: 586 NLIDNSSQDKISTEEKSLFYKIFETFDTQNKGLLDSPTAVEIFRKSGLNRADLEQIWNLCDINNTGQLNKQEFALGMHLVYGKLNGKPIPNVLPSSLIPSST 687 HSP 2 Score: 61.6178 bits (148), Expect = 1.300e-9 Identity = 32/84 (38.10%), Postives = 48/84 (57.14%), Query Frame = 3
Query: 66 LWFITSEEKVKFDVQFQCLNPVNG--FITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVP 311
L FIT++++ KF+ F+ + NG ++G + + +S LP L RIW L D K G + EFA+AMHLI L+G +P
Sbjct: 263 LSFITAQDQAKFETLFRSIV-TNGSNTVSGANCRKILMRSGLPPSQLARIWTLCDTSKAGELLFPEFALAMHLINDVLQGDTIP 345 HSP 3 Score: 52.373 bits (124), Expect = 8.267e-7 Identity = 23/58 (39.66%), Postives = 33/58 (56.90%), Query Frame = 3
Query: 717 NKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSG 890
N ++G + IL++SGLP + LA IW+L D + G L EF +AMHL+ G
Sbjct: 284 NGSNTVSGANCRKILMRSGLPPSQLARIWTLCDTSKAGELLFPEFALAMHLINDVLQG 341
BLAST of Intersectin-1 vs. Ensembl Yeast
Match: EDE1 (Scaffold protein involved in the formation of early endocytic sites; putative regulator of cytokinesis; homo-oligomerization is required for localization to and organization of endocytic sites; has a network of interactions with other endocytic proteins; binds membranes in a ubiquitin-dependent manner; may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death; homolog of mammalian Eps15 [Source:SGD;Acc:S000000143]) HSP 1 Score: 58.5362 bits (140), Expect = 9.692e-9 Identity = 32/86 (37.21%), Postives = 46/86 (53.49%), Query Frame = 3
Query: 69 WFITSEEKVKFDVQFQCLNPVN-GFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLP 323
W ++ E+K +FD F L+ + G ++ F S L + L IW LAD + EFAIAM LI++K G E+PDV+P
Sbjct: 271 WSLSFEKKQQFDAIFDSLDKQHAGSLSSAVLVPFFLSSRLNQETLATIWDLADIHNNAEFTKLEFAIAMFLIQKKNAGVELPDVIP 356 HSP 2 Score: 58.5362 bits (140), Expect = 1.081e-8 Identity = 32/97 (32.99%), Postives = 50/97 (51.55%), Query Frame = 3
Query: 54 NNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEV----PDVLPLSL 332
NN ++ ++S + KF F + G++AK +F K+ LP LG IW L D D G ++ +EF +AM+LI+ + + P VLP L
Sbjct: 124 NNTDIPALSSNDIAKFSQLFDRTAKGAQTVAGDKAKDIFLKARLPNQTLGEIWALCDRDASGVLDKSEFIMAMYLIQLCMSHHPSMNTPPAVLPTQL 220 HSP 3 Score: 52.373 bits (124), Expect = 7.967e-7 Identity = 23/72 (31.94%), Postives = 42/72 (58.33%), Query Frame = 3
Query: 75 ITSEEKVKFDVQFQCLNPVN-GFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKR 287
++S+E+ ++ +F L+ + G +TG + LF S LP +L ++W D D G +N NEF+ A+ +I +
Sbjct: 10 LSSQEQAFYNQKFHQLDTEDLGVVTGEAVRPLFASSGLPGQLLSQVWATVDIDNKGFLNLNEFSAALRMIAQ 81 HSP 4 Score: 50.8322 bits (120), Expect = 2.247e-6 Identity = 22/64 (34.38%), Postives = 33/64 (51.56%), Query Frame = 3
Query: 687 YRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQ 878
Y +F+Q D G +TG + + SGLP L+ +W+ DID G L EF A+ ++ Q
Sbjct: 18 YNQKFHQLDTEDLGVVTGEAVRPLFASSGLPGQLLSQVWATVDIDNKGFLNLNEFSAALRMIAQ 81
BLAST of Intersectin-1 vs. Ensembl Yeast
Match: ROM1 (Guanine nucleotide exchange factor (GEF) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP; ROM1 has a paralog, ROM2, that arose from the whole genome duplication [Source:SGD;Acc:S000003302]) HSP 1 Score: 51.6026 bits (122), Expect = 1.413e-6 Identity = 60/269 (22.30%), Postives = 120/269 (44.61%), Query Frame = 3
Query: 3750 LTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDE----LKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKEL-CETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLG----HRKLIHSGTLSKYKSGKELVGFLFN-DFFLL 4526
L++ + +RQ I EL TEK + + L+ + F + + ++N++ D +K +F + +++ + F AL R+ L+ + + DI L P F ++ + + + ++ ++ P F E S+LR + S+ + + R +Y LLV I+ H D D+ D+ + + + L + + + + + Q+ + L +K+ F + LG RK+ H G LS+ K F + F+LL
Sbjct: 458 LSKQQIKRQEAIYELFTTEKKFVKSLEIIRDTFMKKLLETNIIPSDVRINFVKHVFAHINEIYSVNREFLKALAQRQSLSPI---CPGIADIFLQYLPFFDPFLSYIASRPYAKYLIETQRSVNPNFARFDDEVSNSSLR---HGIDSFLSQGVSRPGRYSLLVREII-HFSDPVTDKDDL-QMLMKVQDLLKDLMKRIDRASGAA-----QDRYDVKVLKQKILFKNEYVNLGLNNEKRKIKHEGLLSRKDVNKTDASFSGDIQFYLL 713
BLAST of Intersectin-1 vs. Ensembl Nematostella
Match: EDO44650 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUY7]) HSP 1 Score: 307.375 bits (786), Expect = 1.332e-93 Identity = 164/341 (48.09%), Postives = 229/341 (67.16%), Query Frame = 3
Query: 4218 LLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYN-TGGMPFVFPLETSSKDKMQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDL 5231
L + ILKHT D HPDR ++E A+ A LC VNEGVR +ENS+RLEWLQ H++ E L E+L FNS TNCLG RKL+HSGTL K KSGKEL FLFNDF LL P TG + E S+ +Q+ +Y+KPI LN+V++ + +S FS + I V + +A S+ DR++W+E+L A+ YI TE R+ R RT+G+G+L+VT+VEG+ L+ SS SDP+CE S+G+Q H+T+V P TLNPKWN+ M F +K+++QDVLC+TV+++D+F+PNDFLGR EVS+ +++ + + P +RL L EV GE++V+L+L
Sbjct: 28 LYLSEILKHTPDIHPDRMNLEIALEKAEELCNQVNEGVRSQENSDRLEWLQNHVKREGLGEQLIFNSATNCLGPRKLLHSGTLFKVKSGKELKAFLFNDFLLLTRPQSTITGTLSKKIGFE-SNDASIQYTIYRKPIFLNEVVVKRPADMNSEEPVFSVSHIDRV------YTFKAESKGDRNRWIEKLEKAAEHYIETERFMRQKMHRTRSIRTSGIGKLVVTIVEGVDLK--SSDPSGMSDPYCEVSMGSQEHKTRVCPQTLNPKWNSTMTFTVKDMEQDVLCITVFDRDFFSPNDFLGRTEVSLASLLKK---GKGPWHERLLLHEVTTGEVLVKLEL 356
BLAST of Intersectin-1 vs. Ensembl Nematostella
Match: EDO44654 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUZ2]) HSP 1 Score: 136.732 bits (343), Expect = 2.456e-37 Identity = 64/128 (50.00%), Postives = 98/128 (76.56%), Query Frame = 3
Query: 4848 RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDL 5231
RT+G+G+L+VT+VEG+ L+SS S P+CE S+G+Q H+T+V P TLNPKWN+ M F +K+++QDVLC+TV+++D+F+PNDFLGR EVS+ +++ + + P +RL L EV GE++V+L+L
Sbjct: 4 RTSGIGKLVVTIVEGVDLKSSDPSGMSD--PYCEVSMGSQEHKTRVCPQTLNPKWNSTMTFTVKDMEQDVLCITVFDRDFFSPNDFLGRTEVSLASLLKKG---KGPWHERLLLHEVTTGEVLVKLEL 126
BLAST of Intersectin-1 vs. Ensembl Nematostella
Match: EDO44656 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUZ5]) HSP 1 Score: 135.961 bits (341), Expect = 5.132e-37 Identity = 63/128 (49.22%), Postives = 98/128 (76.56%), Query Frame = 3
Query: 4848 RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDL 5231
RT+G+G+L+VT+VEG+ ++SS S P+CE S+G+Q H+T+V P TLNPKWN+ M F +K+++QDVLC+TV+++D+F+PNDFLGR EVS+ +++ + + P +RL L EV GE++V+L+L
Sbjct: 4 RTSGIGKLVVTIVEGVDIKSSDPSGMSD--PYCEVSMGSQEHKTRVCPQTLNPKWNSTMTFTVKDMEQDVLCITVFDRDFFSPNDFLGRTEVSLASLLKKG---KGPWHERLLLHEVTTGEVLVKLEL 126
BLAST of Intersectin-1 vs. Ensembl Nematostella
Match: EDO29744 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T2S0]) HSP 1 Score: 130.183 bits (326), Expect = 4.686e-33 Identity = 111/402 (27.61%), Postives = 176/402 (43.78%), Query Frame = 3
Query: 2721 LCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALYYPQNTVPVNSNGASTPEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLI 3926
+CVA Y Y GDL+ + GD+I + + +WW+G R + GLFP+NY + A + S +SLNS+ + S EIA VV + ++A+Q+ L GQLI++ K PSGWW+GE+Q RG++R+ G FP +V I+ + S ST + + G+ ++ M+ Y+AQ DEL+F K ++ ++ K + EWW G L+ Q G+FP NYV+ L AS ++ W A +++ E+ RQ I ELI +E+ Y + L +VF+ P+ +S LLT +E+ +IF+NW ++I
Sbjct: 1 MCVALYTYNGEE-GDLSFKEGDVIGITKDEGEWWEG--RLRENYGLFPANY------------------------------VKKKEAERKSTLSLNSL----FCLFS-------------------------EIATVVSPYTAIAADQLSLAPGQLIRVTRKDPSGWWEGELQARGKKRQSGIFPANHVKILAKGPG---------------------------GSQSGSCTSTQQPPEG--GNQVLAMFPYTAQKGDELTFYKGSVFNVMSK-DGEWWKGE--LNGQVGMFPSNYVQSLGDLPASTKQ----------------------WTGSFDAAILASMSDTERMRQNAIYELINSEQAYMDQLSLTLEVFYNPLAESGLLTANEMNTIFVNWKEII 286 HSP 2 Score: 72.7886 bits (177), Expect = 1.523e-13 Identity = 87/315 (27.62%), Postives = 135/315 (42.86%), Query Frame = 3
Query: 2067 AIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSECQWAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQ-GLFPSNYVKIV------TSEDLANTESAKFAEGVPVEEMSE-SIIDLMNASQ 2987
A+Y + G + D ++FK GD+I I T+D+ E W G L + GLFP YV+K + + K ++ N LF C ++E P + + +QLS GQLI++ + WW GE L A GK++ G FP +V++L + SG+ T T E G+Q L A +PY A +L G + V+ + +WWKG N Q G+FPSNYV+ + T + + ++A A E M + +I +L+N+ Q
Sbjct: 4 ALYTYNGEEGD-LSFKEGDVIGI-TKDEGE----WWEGRL--RENYGLFPANYVKK-------KEAERKSTLSLN------SLF---------------CLFSEIATVVSPYTAIA----ADQLSLAPGQLIRVTRKDPSGWWEGE---LQARGKKRQ-SGIFPANHVKIL-AKGPGGSQSGSCTSTQQPPEG--------------------GNQVL---AMFPYTAQKGDELTFYKGSVFNVMSKDGEWWKG---ELNGQVGMFPSNYVQSLGDLPASTKQWTGSFDAAILASMSDTERMRQNAIYELINSEQ 247
BLAST of Intersectin-1 vs. Ensembl Nematostella
Match: EDO33665 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SRI8]) HSP 1 Score: 124.405 bits (311), Expect = 1.701e-30 Identity = 79/249 (31.73%), Postives = 134/249 (53.82%), Query Frame = 3
Query: 3750 LTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFI------VYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQ---CENLSEKLYFNSNTNCLGHRKLIHSGTLS 4469
L + E +RQ +I ELI TEK + +L+ + +VF +P+KK N++ + +K +F N +LI + ++K R+ V IQ +GDI+L+ F N FC+ Q + +L+++Q+ PK + CE++ C+++ + QR+TKY LL+E I K+T + PD D+E A+ A+ L VN+ V+E EN +RLE Q+ + ++ + K T +KLI+ G L+
Sbjct: 7 LKQKEIKRQEFIHELIYTEKTHVRNLKVLDKVFHKPMKKMNIMPYEYIKKLFPNLQELIEIHGDLLQSMKKREAEGEV---IQCIGDIMLNRFDNEAGEAAKQACATFCNYQKNALEELRQRQKKDPKLLQFIMNCEASPLCRRLKLNEIISTSYQRLTKYPLLLEGIGKNTPSSLPDSRDIERAIQCAKDLLAFVNQSVKECENQQRLEEFQKKVDRKPMDSATNKQMEEFKTLDFSKKKLIYDGPLT 252
BLAST of Intersectin-1 vs. Ensembl Medaka
Match: itsn2a (intersectin-2 [Source:NCBI gene;Acc:101165932]) HSP 1 Score: 447.973 bits (1151), Expect = 4.128e-130 Identity = 247/601 (41.10%), Postives = 354/601 (58.90%), Query Frame = 3
Query: 3453 HLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLL--ATPCYNTGGMPFVFPLETSSKDKMQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMFK 5243
+I MY+Y+A N DELSF K ++ ++DK P+WW G ++ GL P NY++ + S + W D MT + +T E++RQ YI ELI+TE+ Y EDL+ V +VF +P+ +S LT E+ +IF+NW LI ++ F AL+ RK G PIQ +GD+L S Y+ FCS Q A LQ K + P FK+ +N RC+ MP++S+ LKPMQRIT+Y LL++NIL++T ++H D + EA+ A LC VNEGVRE+ENS+RLEW+Q H+QCE E L FNS TNCLG RKL+HSG L K K +EL FLFNDF LL +T ++ G +F +TS+ Q KMYK P+ LN+V++ SS F + I V + L+ + ++R WV+++ AS +I TE KRE + R +T+G+GRLLVT++E L+ + + KS+P+CE ++G Q + ++ T NPKWN QF I ++ QDVLC+TV+ KD F+P+DFLGR EV V + + T+ +RL L EV GE+ V+LDL +++
Sbjct: 1013 QVIAMYDYTAANQDELSFSKGQLINILDKTNPDWWKGE--VNGVTGLLPTNYIKMTTESDPSQQ-----------------------WCAD-LMTLDT-MTPQERKRQGYIHELIQTEETYVEDLELVLEVFHKPMSESGRLTEAEMATIFVNWRKLIMCNHKFLKALRARKKTGGENMPIQLIGDVLASELAQMHPYICFCSGQLNAAALLQSKTNNQPDFKDFLRKIATNYRCKGMPLSSFLLKPMQRITRYPLLIKNILENTPESHADHGPLREALERAEELCFQVNEGVREKENSDRLEWMQSHIQCEGPIENLVFNSLTNCLGPRKLLHSGRLYKTKGTRELWAFLFNDFLLLTHSTKHFSFSGSDKLFSPKTSA----QLKMYKTPLFLNEVLVKMPPDPSSDEPVFHVSHIDRV------YTLKTETLSERATWVQKIKAASEHFIETEKKKREKAYQARSLKTSGIGRLLVTIIEAQELK--ACKPNGKSNPYCELTMGAQCYTSRPASDTPNPKWNFSCQFFINDLYQDVLCITVFEKDQFSPDDFLGRTEVPVATIKKDME-TKGAANRRLLLHEVPTGEVWVKLDLQLYE 1573 HSP 2 Score: 95.9005 bits (237), Expect = 3.197e-19 Identity = 45/98 (45.92%), Postives = 63/98 (64.29%), Query Frame = 3
Query: 30 FQIDLHMLNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLP 323
FQ L+ + +W IT EE+ K D QF L+P+ G+++G QA+ F +S LP VL IW L+D D DG M+ EF+IAM LIK +L+G+ +P LP
Sbjct: 5 FQTALNTI----VWAITPEERGKHDKQFDSLSPILGYVSGEQARKFFLQSGLPASVLAEIWNLSDLDSDGKMDRLEFSIAMKLIKHRLQGWNLPSSLP 98 HSP 3 Score: 95.9005 bits (237), Expect = 3.753e-19 Identity = 56/162 (34.57%), Postives = 89/162 (54.94%), Query Frame = 3
Query: 654 DWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXKKNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAIL 1139
DW +PQ+ R +YR QFN D+ G+L+G + + L+ S L T LA+IW LAD+D+DG L +EF +AMHLV+ A++G P ++++ S+ + + G T S+ E++ ++ ED+ + N+ +G AELEKRR L
Sbjct: 244 DWAVPQASRLKYRQQFNTLDKLMSGYLSGPQVRNALMASNLTQTQLATIWYLADVDKDGQLRADEFILAMHLVDMAKTG----------YPLPLTLPQDLVPPSLRGGIKLVELVNGTGPCTTPSLIDTAETEPSQKVSYEDKLKENFARGSAELEKRRLAL 395 HSP 4 Score: 73.559 bits (179), Expect = 2.290e-12 Identity = 34/91 (37.36%), Postives = 53/91 (58.24%), Query Frame = 3
Query: 69 WFITSEEKVKFDVQFQCLNPV-NGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLPLSLNP 338
W + ++K+ QF L+ + +G+++G Q ++ SNL L IW+LAD DKDG + ++EF +AMHL+ GY +P LP L P
Sbjct: 245 WAVPQASRLKYRQQFNTLDKLMSGYLSGPQVRNALMASNLTQTQLATIWYLADVDKDGQLRADEFILAMHLVDMAKTGYPLPLTLPQDLVP 335 HSP 5 Score: 59.6918 bits (143), Expect = 3.763e-8 Identity = 78/292 (26.71%), Postives = 121/292 (41.44%), Query Frame = 3
Query: 2046 NELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHC---GLFPEIYVRKLSGPPSTESTQT---KIDVNQNISSGFADLFNNDITPKGTLRKSSECQWAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNL-SVKYDLNTFDGSQKLLCV-ARYPYQASVLGDLNIQPGDIIEVLDQKQ-DWWKG--YNRNTNSQ-GLFPSNYVKIV 2885
+L Y+A+Y+F R DE++ +I +N EP GWL G ++C G FP+ Y K ++E+ K Q + A + I + S A AI W S V LS +I ++ D+WW G+ NG + GWFP++YV L + + + D P A L A K L L S Y + + + +Q ++ A P+Q L + PG +I + + WW G R Q G F S++VK++
Sbjct: 741 GQLTTYKALYSFVARNADELSIDANCLIQVNELTVGEP--GWLCG-----TYCGKRGWFPQSYAEKCPTFLTSEAAAPAAGKKSCLQPNTKYEAKVKQFWIFSLKVVSSCSVTLLARAISSWSATSGVL-------LSLSQDDIITVLQQR-DEWWLGQL-----NGTQ----GWFPRSYVTLEMDGSGEYDD-----PEASRLGASAKKPVFLLLISDLYSVWSTEIAQSVVTAEAPAPHQ------LLLSPGQLIVICAKNSTGWWLGELQARGKKPQRGWFHSSHVKLL 997 HSP 6 Score: 58.151 bits (139), Expect = 1.088e-7 Identity = 29/78 (37.18%), Postives = 45/78 (57.69%), Query Frame = 3
Query: 657 WVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSG 890
W I R ++ QF+ G+++G +A+ +QSGLP + LA IW+L+D+D DG + EF IAM L++ G
Sbjct: 14 WAITPEERGKHDKQFDSLS-PILGYVSGEQARKFFLQSGLPASVLAEIWNLSDLDSDGKMDRLEFSIAMKLIKHRLQG 90
BLAST of Intersectin-1 vs. Ensembl Medaka
Match: ENSORLT00000014039.2 (pep primary_assembly:ASM223467v1:3:24695019:24701881:-1 gene:ENSORLG00000011188.2 transcript:ENSORLT00000014039.2 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 391.349 bits (1004), Expect = 1.594e-120 Identity = 209/497 (42.05%), Postives = 316/497 (63.58%), Query Frame = 3
Query: 3762 EKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLL--ATPCYNTGGMPFVFPLETSSKDKMQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMF 5240
EK+RQ YI EL++ E+ Y +DLQ V +VF +P+ +S LT E+ IF+NW +L+ + AL++RK G P+Q +GDIL + + Y+ FCS Q G + LQ + + P FKE + ++ RC+ MP++S+ LKPMQRIT+Y L ++NIL T + H DR ++EA+ A LC+ VNEGVRE+ENS+RLEW+Q H+QC+ +E L FNS TNCLG RKL+HSG + K KS KEL FLFNDF LL A +++ G +F S+++ +Q KMYK P++LN+++ + S +SS F I V ++L+ + N+R WV+++ AS +I TE KRE + R + +G+GRLLVT++E L+ ++ + KS+P+CE ++G Q+ ++ + TLNPKWN QF +K++ QDVLC+T+ ++ F+P++FLGR E+ V + E + + +RL L EV GE+ VRLDL ++
Sbjct: 25 EKKRQGYIHELMDAEEKYVDDLQVVLEVFHKPMSESGRLTDSEMAMIFVNWKELLACNTKLVKALRVRKKTTGENMPVQMIGDILAAELSHMQPYISFCSCQINGASLLQTRTDNEPDFKEFLKKLGTDYRCKGMPLSSFLLKPMQRITRYPLHIKNILDSTIEGHADRGPLKEALERAEELCQQVNEGVREKENSDRLEWIQNHVQCDGPAENLVFNSLTNCLGPRKLLHSGKMYKAKSNKELWAFLFNDFLLLTHAAKQFSSSGPDKLF----SNRNNVQLKMYKPPVLLNEIL-AKISDSSSDEPTFQITHIDRV------YVLKTENINERTAWVQKIKAASEEFIETELKKREKAYQGRSIKVSGIGRLLVTILEATELK--ATRPNGKSNPYCEVNVGAQVFTSRTLNDTLNPKWNFSCQFHLKDLYQDVLCITISDRAQFSPDEFLGRTEIPVATIKKEME-NKGSVTRRLLLHEVPTGEVWVRLDLQLY 507
BLAST of Intersectin-1 vs. Ensembl Medaka
Match: ENSORLT00000027169.1 (pep primary_assembly:ASM223467v1:15:636421:646545:1 gene:ENSORLG00000030643.1 transcript:ENSORLT00000027169.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 210.305 bits (534), Expect = 1.382e-60 Identity = 122/290 (42.07%), Postives = 168/290 (57.93%), Query Frame = 3
Query: 4401 KLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYNTGGMPFVFPLETSSKDK------MQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIR--RTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPNDFLGRIEVSVNNVVTEKRITRDPIEKRLKLMEVEQGELVVRLDLAMFKK 5246
+L FNS TNCLG RK +HSG L K KS KEL GFLFNDF LL + PL +S DK +Q++MYK PI LN+V++ + S F + I V + LRA S N+R WV+++ AS +I TE KRE + +R R G+GRL+V +VEGI L+ C + L + TLNPKWN+ QF IK+++QDVLC+TV+ +D F+P+DFLGR E+ + + ++ ++ PI KRL L EV GE+VVRLDL +F++
Sbjct: 11 QLVFNSVTNCLGPRKFLHSGKLFKAKSSKELYGFLFNDFLLLTQ---------VIKPLGSSGSDKVFSKTHLQYRMYKTPIFLNEVLVKLPTDPSGDEPLFHISHIDRV------YTLRAESINERTAWVQKIKAASELFIETEKKKREKAYLVRSQRATGIGRLMVNIVEGIELKP------------CRSHGTKTLGVHTIRTDTLNPKWNSNCQFFIKDLEQDVLCVTVFERDQFSPDDFLGRTEIRLAEIKKDQG-SKGPITKRLLLHEVPTGEIVVRLDLQLFEE 272
BLAST of Intersectin-1 vs. Ensembl Medaka
Match: ENSORLT00000013993.2 (pep primary_assembly:ASM223467v1:15:553091:605492:1 gene:ENSORLG00000011152.2 transcript:ENSORLT00000013993.2 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 176.792 bits (447), Expect = 1.195e-45 Identity = 140/385 (36.36%), Postives = 202/385 (52.47%), Query Frame = 3
Query: 54 NNPNLWFITSEEKVKFDVQFQCLNP-VNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLPLSLN----PLQTNATTMSYSGYMPVS------NTYNTM-PVMHNTS-----HAMSNNNPNNTMAVPHASAFVNYXXXXXXXXXXXXXXXXNAQFGSXXXXXXXXXXSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXKKNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAILDEQMRQ 1157
+ P+++ I+ +E+ K D QF L+P GFITG+QA++ F +S L +L +IW LAD + DG M+ +EF+IAM LIK KL+G+ +P LP S+ PL TT + P S N+ M P + +T+ ++N P T +P S F ++ +S + F S++ S PQ PP DW +PQS R +YR FN D+ G LTG +A+ IL+QS LP LA+IWSL+DIDQDG LT EEF +AMHL++ A SG LP LP LPP+ + + S+ + + +SE ++K +T ED++R N+ +G ELEKRR L EQ R+
Sbjct: 13 SGPDVFTISVDERAKHDQQFHGLSPSAGGFITGDQARNFFLQSGLSPPILAQIWALADMNSDGRMDIHEFSIAMKLIKLKLQGHPLPSALPPSMKQPLLPLPPQ-TTFGECSFTPKSLSNCADNSVVFMSPPLVSTAPPPLPQPIANGAPP-TALMPPLSGF-------SLPGKLLLVSNLHCVFYSSSFC------SAPSPQ----PPT-------DWAVPQSSRLKYRQLFNSHDKMMSGHLTGPQARTILMQSSLPQGQLATIWSLSDIDQDGKLTAEEFILAMHLIDMAMSGLPLPPVLPPEYLPPTF--RRVRSDSVQSDQ--------KSIQEEVEDESESCQEKKLPVTFEDKKRENFERGNLELEKRRQALQEQQRK 361
BLAST of Intersectin-1 vs. Ensembl Medaka
Match: net1 (neuroepithelial cell transforming 1 [Source:NCBI gene;Acc:101166022]) HSP 1 Score: 102.064 bits (253), Expect = 1.188e-21 Identity = 81/298 (27.18%), Postives = 140/298 (46.98%), Query Frame = 3
Query: 3762 EKRRQFYIRELIETEKVYYEDLQEVHQVFFEPIKKSNLLTLDELKSIFLNWSDLIPNSYNFFMALKIRKDLAGVKQPIQSVGDILLSNFPNFIVYVRFCSMQNQGIAKLQKKQQSCPKFKELCETCESNLRCQKMPMTSYFLKPMQRITKYKLLVENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENS---ERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYNTGGMPFVFPLETSSKDKMQFKMYKKPIILNQVMI 4646
E +RQ I EL E+ EDLQ + + +P+ K ++++ EL IF N IP L G + +G I+++ P Y +CS Q A L +K+Q P+ ++ + C + +K+ + S+ P R+ KY LL++ IL+HT +HPD+ +EEA+ + +N E E E+LE+L+E Q + L E+ R L+ G L + KSG +L FLF + +L P + ++ F++Y++PI ++ +++
Sbjct: 93 EIKRQEAIFELSRGEQDLIEDLQLARKAYHDPMLKLSIMSEVELTHIFGNLDAYIPLHEELLAQLSKATRPDGT---VGQIGQIVINWLPRLDAYKNYCSNQLAAKALLDQKKQD-PRVQDFLQRCLESPFSRKLDLWSFLDIPRSRLVKYPLLLKEILRHTPVDHPDKGSLEEAITIIHGVLSDINVKKGESECQYYIEKLEYLEER-QKDPLMEQC-----------RSLLCHGEL-RNKSGTKLHVFLFTELLVLTRP--------------VTRNERQCFQVYRQPIPVHDLVL 359
BLAST of Intersectin-1 vs. Planmine SMEST
Match: SMESG000066021.1 (SMESG000066021.1) HSP 1 Score: 2098.94 bits (5437), Expect = 0.000e+0 Identity = 1216/1255 (96.89%), Postives = 1223/1255 (97.45%), Query Frame = 3
Query: 48 MLNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLPLSLNPLQTNATTMSYSGYMPVSNTYNTMPVMHNTSHAMSNNNPNNTMAVPHASAFVNYXXXXXXXXXXXXXXXXNAQFGSXXXXXXXXXXSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXKKNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAILDEQMRQXXXXXXXXXXXXXXXXXXXXQEKEQARFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKMRLESEKKRLLEMEKRRLEVAIXXXXXXXXXXXXVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYKAGIGSMREHRDKLIDTTDCXXXXXXXXXXXXSRCQREKXXXXXXXXXXXXXDPVLEQIKTVQALKSSHXXXXXXXXXXXXXXXQRMTHRGQKLATNRNKVRDLEKKVTSKLLSDIKKLQTSVCEKKNAAIAILKKQKEPKTAVSEKNYNEFDKIGISKNPQLNSMPSNELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSECQWAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALYYPQNTVPVNSNGASTPEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKV 3812
MLNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYE+PDVLPLSLNPLQTNATTMSYSGYMPVSNTYNTMPVMHNTSHAMSNNNPNNTMAVPHASAFVNYSTSSTMKSFSTSLSTSNAQFGSNNIPFPINNPSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRQLPNQLPQSLLPPSLQKKNILGTSMSASMNIPINPITNGVSVTKSIQSEKESQQKSVLTGEDERRANYLKGQAELEKRRAILDEQMRQEEEGRKAKERAEIERLEKIRQEKEQARFAEIQRQMEIRRQQELEREEQLRRVAEEREKMRLESEKKRLLEMEKRRLEVAISERRQEQESLSEVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYKAGIGSMREHRDKLIDTTDCLREELEAAKAELSRCQREKQNLELQLQSNLQSDPVLEQIKTVQALKSSH + ++ R K+VTSKLLSDIKKLQTSVCEKKNAAIAILKKQKEPKTAVSEKNYNEFDKIGISKNPQLNSMPSNELVYYEAIYAFEGRQHDEI+FKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSECQWAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALYYPQNTVPVNSNGASTPEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEIQQRGQQRRIGWFPGQYVSIINSSQSIVSDSMYNDGSLTPPTCTTPTPMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEK+
Sbjct: 1 MLNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYELPDVLPLSLNPLQTNATTMSYSGYMPVSNTYNTMPVMHNTSHAMSNNNPNNTMAVPHASAFVNYSTSSTMKSFSTSLSTSNAQFGSNNIPFPINNPSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRQLPNQLPQSLLPPSLQKKNILGTSMSASMNIPINPITNGVSVTKSIQSEKESQQKSVLTGEDERRANYLKGQAELEKRRAILDEQMRQEEEGRKAKERAEIERLEKIRQEKEQARFAEIQRQMEIRRQQELEREEQLRRVAEEREKMRLESEKKRLLEMEKRRLEVAISERRQEQESLSEVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYKAGIGSMREHRDKLIDTTDCLREELEAAKAELSRCQREKQNLELQLQSNLQSDPVLEQIKTVQALKSSH--------------KDKINQR-------------FRKEVTSKLLSDIKKLQTSVCEKKNAAIAILKKQKEPKTAVSEKNYNEFDKIGISKNPQLNSMPSNELVYYEAIYAFEGRQHDEISFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSECQWAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALYYPQNTVPVNSNGASTPEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEIQQRGQQRRIGWFPGQYVSIINSSQSIVSDSMYNDGSLTPPTCTTPTPMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKI 1228 HSP 2 Score: 591.267 bits (1523), Expect = 0.000e+0 Identity = 290/292 (99.32%), Postives = 290/292 (99.32%), Query Frame = 3
Query: 4224 VENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYNTGGMPFVFPLETSSKDKMQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIRRTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPN 5099
E ILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYNTGGMPFVFPLETSSKDKMQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIRRTAGVGRLLVTVVEGISLRSSSSHKDSKSDPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPN
Sbjct: 1225 TEKILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYNTGGMPFVFPLETSSKDKMQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIRRTAGVGRLLVTVVEGISLRSSSSHKDSKSDPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPN 1516
BLAST of Intersectin-1 vs. Planmine SMEST
Match: SMESG000066021.1 (SMESG000066021.1) HSP 1 Score: 2031.53 bits (5262), Expect = 0.000e+0 Identity = 1184/1222 (96.89%), Postives = 1190/1222 (97.38%), Query Frame = 3
Query: 48 MLNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLPLSLNPLQTNATTMSYSGYMPVSNTYNTMPVMHNTSHAMSNNNPNNTMAVPHASAFVNYXXXXXXXXXXXXXXXXNAQFGSXXXXXXXXXXSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXKKNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAILDEQMRQXXXXXXXXXXXXXXXXXXXXQEKEQARFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKMRLESEKKRLLEMEKRRLEVAIXXXXXXXXXXXXVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYKAGIGSMREHRDKLIDTTDCXXXXXXXXXXXXSRCQREKXXXXXXXXXXXXXDPVLEQIKTVQALKSSHXXXXXXXXXXXXXXXQRMTHRGQKLATNRNKVRDLEKKVTSKLLSDIKKLQTSVCEKKNAAIAILKKQKEPKTAVSEKNYNEFDKIGISKNPQLNSMPSNELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSECQWAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALYYPQNTVPVNSNGASTPEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVS 3713
MLNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYE+PDVLPLSLNPLQTNATTMSYSGYMPVSNTYNTMPVMHNTSHAMSNNNPNNTMAVPHASAFVNYSTSSTMKSFSTSLSTSNAQFGSNNIPFPINNPSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRQLPNQLPQSLLPPSLQKKNILGTSMSASMNIPINPITNGVSVTKSIQSEKESQQKSVLTGEDERRANYLKGQAELEKRRAILDEQMRQEEEGRKAKERAEIERLEKIRQEKEQARFAEIQRQMEIRRQQELEREEQLRRVAEEREKMRLESEKKRLLEMEKRRLEVAISERRQEQESLSEVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYKAGIGSMREHRDKLIDTTDCLREELEAAKAELSRCQREKQNLELQLQSNLQSDPVLEQIKTVQALKSSH + ++ R K+VTSKLLSDIKKLQTSVCEKKNAAIAILKKQKEPKTAVSEKNYNEFDKIGISKNPQLNSMPSNELVYYEAIYAFEGRQHDEI+FKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSECQWAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALYYPQNTVPVNSNGASTPEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEIQQRGQQRRIGWFPGQYVSIINSSQSIVSDSMYNDGSLTPPTCTTPTPMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVS
Sbjct: 1 MLNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYELPDVLPLSLNPLQTNATTMSYSGYMPVSNTYNTMPVMHNTSHAMSNNNPNNTMAVPHASAFVNYSTSSTMKSFSTSLSTSNAQFGSNNIPFPINNPSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRQLPNQLPQSLLPPSLQKKNILGTSMSASMNIPINPITNGVSVTKSIQSEKESQQKSVLTGEDERRANYLKGQAELEKRRAILDEQMRQEEEGRKAKERAEIERLEKIRQEKEQARFAEIQRQMEIRRQQELEREEQLRRVAEEREKMRLESEKKRLLEMEKRRLEVAISERRQEQESLSEVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYKAGIGSMREHRDKLIDTTDCLREELEAAKAELSRCQREKQNLELQLQSNLQSDPVLEQIKTVQALKSSH--------------KDKINQR-------------FRKEVTSKLLSDIKKLQTSVCEKKNAAIAILKKQKEPKTAVSEKNYNEFDKIGISKNPQLNSMPSNELVYYEAIYAFEGRQHDEISFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSSECQWAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPTAGSLEDLASKYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALYYPQNTVPVNSNGASTPEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEIQQRGQQRRIGWFPGQYVSIINSSQSIVSDSMYNDGSLTPPTCTTPTPMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVS 1195
BLAST of Intersectin-1 vs. Planmine SMEST
Match: SMESG000066021.1 (SMESG000066021.1) HSP 1 Score: 1192.95 bits (3085), Expect = 0.000e+0 Identity = 780/995 (78.39%), Postives = 826/995 (83.02%), Query Frame = 3
Query: 48 MLNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLPLSLNPLQTNATTMSYSGYMPVSNTYNTMPVMHNTSHAMSNNNPNNTMAVPHASAFVNYXXXXXXXXXXXXXXXXNAQFGSXXXXXXXXXXSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXKKNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAILDEQMRQXXXXXXXXXXXXXXXXXXXXQEKEQARFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKMRLESEKKRLLEMEKRRLEVAIXXXXXXXXXXXXVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYKAGIGSMREHRDKLIDTTDCXXXXXXXXXXXXSRCQREKXXXXXXXXXXXXXDPVLEQIKTVQALKSSHXXXXXXXXXXXXXXXQRMTHRGQKLATNRNKVRDLEKKVTSKLLSDIKKLQTSVCEKKNAAIAILKKQKEPKTAVSEKNYNEFDKIGISKNPQLNSMPSNELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSS---ECQWAEAIYHWKPESNVTYGEYPNQ------LSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGT------VTPTAGSLEDL--ASKYSSLNL------------------SVKYDLNTFDGSQKLLCVARYPYQASVLGDLN--------IQPGDIIEVLDQK-QDWWKGYNRNTNSQ---GLFPSNYVKIVTS 2891
MLNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYE+PDVLPLSLNPLQTNATTMSYSGYMPVSNTYNTMPVMHNTSHAMSNNNPNNTMAVPHASAFVNYSTSSTMKSFSTSLSTSNAQFGSNNIPFPINNPSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRQLPNQLPQSLLPPSLQKKNILGTSMSASMNIPINPITNGVSVTKSIQSEKESQQKSVLTGEDERRANYLKGQAELEKRRAILDEQMRQEEEGRKAKERAEIERLEKIRQEKEQARFAEIQRQMEIRRQQELEREEQLRRVAEEREKMRLESEKKRLLEMEKRRLEVAISERRQEQESLSEVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYKAGIGSMREHRDKLIDTTDCLREELEAAKAELSRCQREKQNLELQLQSNLQSDPVLEQIKTVQALKSSH + ++ R K+VTSKLLSDIKKLQTSVCEKKNAAIAILKKQKEPKTAVSEKNYNEFDKIGISKNPQLNSMPSNELVYYEAIYAFEGRQHDEI+FKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLR SS ++ + + + YP Q L+ + G +I+++D DWW G ++ G FP YV+++ ++ + +S V + S+ DL AS++S+++L ++ Y NT + A P A V+ + N +Q G +I++ ++ WWKG + Q G FP YV I+ S
Sbjct: 1 MLNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYELPDVLPLSLNPLQTNATTMSYSGYMPVSNTYNTMPVMHNTSHAMSNNNPNNTMAVPHASAFVNYSTSSTMKSFSTSLSTSNAQFGSNNIPFPINNPSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRQLPNQLPQSLLPPSLQKKNILGTSMSASMNIPINPITNGVSVTKSIQSEKESQQKSVLTGEDERRANYLKGQAELEKRRAILDEQMRQEEEGRKAKERAEIERLEKIRQEKEQARFAEIQRQMEIRRQQELEREEQLRRVAEEREKMRLESEKKRLLEMEKRRLEVAISERRQEQESLSEVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYKAGIGSMREHRDKLIDTTDCLREELEAAKAELSRCQREKQNLELQLQSNLQSDPVLEQIKTVQALKSSH--------------KDKINQR-------------FRKEVTSKLLSDIKKLQTSVCEKKNAAIAILKKQKEPKTAVSEKNYNEFDKIGISKNPQLNSMPSNELVYYEAIYAFEGRQHDEISFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRNSSLNLSVKYDLNTFDGS-QKLLCVARYPYQASVLGDLNIQPGDIIEVLDQK-QDWWKGYNRNTNS-------QGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALYYPQNTVPVNSN---GASTPEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEIQQRGQQRRIGWFPGQYVSIINS 956 HSP 2 Score: 657.907 bits (1696), Expect = 0.000e+0 Identity = 383/475 (80.63%), Postives = 399/475 (84.00%), Query Frame = 3
Query: 2355 SSECQWAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTN-----LDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPT--------AGSLEDLAS---------KYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALYYPQNTVPVNSNGASTPEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVS 3713
S+E + EAIY ++ G +++SF+TG +I II+T W G LD + G FP+ YVR L + P S T T + DL + + SSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALYYPQNTVPVNSNGASTPEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEIQQRGQQRRIGWFPGQYVSIINSSQSIVSDSMYNDGSLTPPTCTTPTPMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVS
Sbjct: 639 SNELVYYEAIYAFE-------GRQHDEISFKTGDII-IINTQDQIEPSSGWLYG---WLDNSSH----CGLFPEIYVRKL-----SGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRNSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALYYPQNTVPVNSNGASTPEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEIQQRGQQRRIGWFPGQYVSIINSSQSIVSDSMYNDGSLTPPTCTTPTPMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVS 1093
BLAST of Intersectin-1 vs. Planmine SMEST
Match: SMESG000066021.1 (SMESG000066021.1) HSP 1 Score: 1188.71 bits (3074), Expect = 0.000e+0 Identity = 780/995 (78.39%), Postives = 826/995 (83.02%), Query Frame = 3
Query: 48 MLNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLPLSLNPLQTNATTMSYSGYMPVSNTYNTMPVMHNTSHAMSNNNPNNTMAVPHASAFVNYXXXXXXXXXXXXXXXXNAQFGSXXXXXXXXXXSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXKKNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAILDEQMRQXXXXXXXXXXXXXXXXXXXXQEKEQARFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKMRLESEKKRLLEMEKRRLEVAIXXXXXXXXXXXXVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYKAGIGSMREHRDKLIDTTDCXXXXXXXXXXXXSRCQREKXXXXXXXXXXXXXDPVLEQIKTVQALKSSHXXXXXXXXXXXXXXXQRMTHRGQKLATNRNKVRDLEKKVTSKLLSDIKKLQTSVCEKKNAAIAILKKQKEPKTAVSEKNYNEFDKIGISKNPQLNSMPSNELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSS---ECQWAEAIYHWKPESNVTYGEYPNQ------LSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGT------VTPTAGSLEDL--ASKYSSLNL------------------SVKYDLNTFDGSQKLLCVARYPYQASVLGDLN--------IQPGDIIEVLDQK-QDWWKGYNRNTNSQ---GLFPSNYVKIVTS 2891
MLNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYE+PDVLPLSLNPLQTNATTMSYSGYMPVSNTYNTMPVMHNTSHAMSNNNPNNTMAVPHASAFVNYSTSSTMKSFSTSLSTSNAQFGSNNIPFPINNPSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRQLPNQLPQSLLPPSLQKKNILGTSMSASMNIPINPITNGVSVTKSIQSEKESQQKSVLTGEDERRANYLKGQAELEKRRAILDEQMRQEEEGRKAKERAEIERLEKIRQEKEQARFAEIQRQMEIRRQQELEREEQLRRVAEEREKMRLESEKKRLLEMEKRRLEVAISERRQEQESLSEVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYKAGIGSMREHRDKLIDTTDCLREELEAAKAELSRCQREKQNLELQLQSNLQSDPVLEQIKTVQALKSSH + ++ R K+VTSKLLSDIKKLQTSVCEKKNAAIAILKKQKEPKTAVSEKNYNEFDKIGISKNPQLNSMPSNELVYYEAIYAFEGRQHDEI+FKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLR SS ++ + + + YP Q L+ + G +I+++D DWW G ++ G FP YV+++ ++ + +S V + S+ DL AS++S+++L ++ Y NT + A P A V+ + N +Q G +I++ ++ WWKG + Q G FP YV I+ S
Sbjct: 1 MLNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYELPDVLPLSLNPLQTNATTMSYSGYMPVSNTYNTMPVMHNTSHAMSNNNPNNTMAVPHASAFVNYSTSSTMKSFSTSLSTSNAQFGSNNIPFPINNPSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRQLPNQLPQSLLPPSLQKKNILGTSMSASMNIPINPITNGVSVTKSIQSEKESQQKSVLTGEDERRANYLKGQAELEKRRAILDEQMRQEEEGRKAKERAEIERLEKIRQEKEQARFAEIQRQMEIRRQQELEREEQLRRVAEEREKMRLESEKKRLLEMEKRRLEVAISERRQEQESLSEVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYKAGIGSMREHRDKLIDTTDCLREELEAAKAELSRCQREKQNLELQLQSNLQSDPVLEQIKTVQALKSSH--------------KDKINQR-------------FRKEVTSKLLSDIKKLQTSVCEKKNAAIAILKKQKEPKTAVSEKNYNEFDKIGISKNPQLNSMPSNELVYYEAIYAFEGRQHDEISFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRNSSLNLSVKYDLNTFDGS-QKLLCVARYPYQASVLGDLNIQPGDIIEVLDQK-QDWWKGYNRNTNS-------QGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALYYPQNTVPVNSN---GASTPEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEIQQRGQQRRIGWFPGQYVSIINS 956 HSP 2 Score: 726.087 bits (1873), Expect = 0.000e+0 Identity = 415/508 (81.69%), Postives = 432/508 (85.04%), Query Frame = 3
Query: 2355 SSECQWAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTN-----LDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPT--------AGSLEDLAS---------KYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALYYPQNTVPVNSNGASTPEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKV 3812
S+E + EAIY ++ G +++SF+TG +I II+T W G LD + G FP+ YVR L + P S T T + DL + + SSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALYYPQNTVPVNSNGASTPEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEIQQRGQQRRIGWFPGQYVSIINSSQSIVSDSMYNDGSLTPPTCTTPTPMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEK+
Sbjct: 639 SNELVYYEAIYAFE-------GRQHDEISFKTGDII-IINTQDQIEPSSGWLYG---WLDNSSH----CGLFPEIYVRKL-----SGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRNSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALYYPQNTVPVNSNGASTPEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEIQQRGQQRRIGWFPGQYVSIINSSQSIVSDSMYNDGSLTPPTCTTPTPMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKI 1126 HSP 3 Score: 592.423 bits (1526), Expect = 0.000e+0 Identity = 290/292 (99.32%), Postives = 290/292 (99.32%), Query Frame = 3
Query: 4224 VENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYNTGGMPFVFPLETSSKDKMQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIRRTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPN 5099
E ILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYNTGGMPFVFPLETSSKDKMQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIRRTAGVGRLLVTVVEGISLRSSSSHKDSKSDPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPN
Sbjct: 1123 TEKILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYNTGGMPFVFPLETSSKDKMQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIRRTAGVGRLLVTVVEGISLRSSSSHKDSKSDPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPN 1414
BLAST of Intersectin-1 vs. Planmine SMEST
Match: SMESG000066021.1 (SMESG000066021.1) HSP 1 Score: 1179.85 bits (3051), Expect = 0.000e+0 Identity = 757/893 (84.77%), Postives = 790/893 (88.47%), Query Frame = 3
Query: 48 MLNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYEVPDVLPLSLNPLQTNATTMSYSGYMPVSNTYNTMPVMHNTSHAMSNNNPNNTMAVPHASAFVNYXXXXXXXXXXXXXXXXNAQFGSXXXXXXXXXXSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRXXXXXXXXXXXXXXXXKKNILGTSMSASMNIPINPITNGVSVTXXXXXXXXXXXXXVLTGEDERRANYLKGQAELEKRRAILDEQMRQXXXXXXXXXXXXXXXXXXXXQEKEQARFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKMRLESEKKRLLEMEKRRLEVAIXXXXXXXXXXXXVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYKAGIGSMREHRDKLIDTTDCXXXXXXXXXXXXSRCQREKXXXXXXXXXXXXXDPVLEQIKTVQALKSSHXXXXXXXXXXXXXXXQRMTHRGQKLATNRNKVRDLEKKVTSKLLSDIKKLQTSVCEKKNAAIAILKKQKEPKTAVSEKNYNEFDKIGISKNPQLNSMPSNELVYYEAIYAFEGRQHDEITFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRKSS---ECQWAEAIYHWKPESNVTYGEYPNQ------LSFETGQLIKIIDTNLDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGT------VTPTAGSLEDL--ASKYSSLNL 2675
MLNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYE+PDVLPLSLNPLQTNATTMSYSGYMPVSNTYNTMPVMHNTSHAMSNNNPNNTMAVPHASAFVNYSTSSTMKSFSTSLSTSNAQFGSNNIPFPINNPSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRQLPNQLPQSLLPPSLQKKNILGTSMSASMNIPINPITNGVSVTKSIQSEKESQQKSVLTGEDERRANYLKGQAELEKRRAILDEQMRQEEEGRKAKERAEIERLEKIRQEKEQARFAEIQRQMEIRRQQELEREEQLRRVAEEREKMRLESEKKRLLEMEKRRLEVAISERRQEQESLSEVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYKAGIGSMREHRDKLIDTTDCLREELEAAKAELSRCQREKQNLELQLQSNLQSDPVLEQIKTVQALKSSH + ++ R K+VTSKLLSDIKKLQTSVCEKKNAAIAILKKQKEPKTAVSEKNYNEFDKIGISKNPQLNSMPSNELVYYEAIYAFEGRQHDEI+FKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLR SS ++ + + + YP Q L+ + G +I+++D DWW G ++ G FP YV+++ ++ + +S V + S+ DL AS++S+++L
Sbjct: 1 MLNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVLGRIWFLADYDKDGCMNSNEFAIAMHLIKRKLEGYELPDVLPLSLNPLQTNATTMSYSGYMPVSNTYNTMPVMHNTSHAMSNNNPNNTMAVPHASAFVNYSTSSTMKSFSTSLSTSNAQFGSNNIPFPINNPSYAVPQDINKPPIHTVTSDDDWVIPQSVRPRYRLQFNQQDRNKRGFLTGVEAKAILVQSGLPNTSLASIWSLADIDQDGNLTCEEFCIAMHLVEQARSGRQLPNQLPQSLLPPSLQKKNILGTSMSASMNIPINPITNGVSVTKSIQSEKESQQKSVLTGEDERRANYLKGQAELEKRRAILDEQMRQEEEGRKAKERAEIERLEKIRQEKEQARFAEIQRQMEIRRQQELEREEQLRRVAEEREKMRLESEKKRLLEMEKRRLEVAISERRQEQESLSEVNSKKGNLIDALANEKEKKRDLQSRIESTQITLETYKAGIGSMREHRDKLIDTTDCLREELEAAKAELSRCQREKQNLELQLQSNLQSDPVLEQIKTVQALKSSH--------------KDKINQR-------------FRKEVTSKLLSDIKKLQTSVCEKKNAAIAILKKQKEPKTAVSEKNYNEFDKIGISKNPQLNSMPSNELVYYEAIYAFEGRQHDEISFKTGDIIIINTQDQIEPSSGWLYGWLDNSSHCGLFPEIYVRKLSGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRNSSLNLSVKYDLNTFDGS-QKLLCVARYPYQASVLGDLNIQPGDIIEVLDQK-QDWWKGYNRNTNS-------QGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISL 857 HSP 2 Score: 681.404 bits (1757), Expect = 0.000e+0 Identity = 382/508 (75.20%), Postives = 399/508 (78.54%), Query Frame = 3
Query: 2355 SSECQWAEAIYHWKPESNVTYGEYPNQLSFETGQLIKIIDTN-----LDDWWTGETTALDANGKEKIIMGWFPKTYVRLLDEQNANHPDSGTVTPT--------AGSLEDLAS---------KYSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALYYPQNTVPVNSNGASTPEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPSGWWKGEXXXXXXXXXXXWFPGQYVXXXXXXXXXXXXXMYNDGSLXXXXXXXXXXMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKV 3812
S+E + EAIY ++ G +++SF+TG +I II+T W G LD + G FP+ YVR L + P S T T + DL + + SSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALYYPQNTVPVNSNGASTPEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPS VSDSMYNDGSLTPPTCTTPTPMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEK+
Sbjct: 639 SNELVYYEAIYAFE-------GRQHDEISFKTGDII-IINTQDQIEPSSGWLYG---WLDNSSH----CGLFPEIYVRKL-----SGPPSTESTQTKIDVNQNISSGFADLFNNDITPKGTLRNSSLNLSVKYDLNTFDGSQKLLCVARYPYQASVLGDLNIQPGDIIEVLDQKQDWWKGYNRNTNSQGLFPSNYVKIVTSEDLANTESAKFAEGVPVEEMSESIIDLMNASQHSAISLNSVTSNPYSTISDVENNSALYYPQNTVPVNSNGASTPEIAQVVIEFNPLSAEQIPLQVGQLIQIREKSPS---------------------------------VSDSMYNDGSLTPPTCTTPTPMSSWINQDTSSINSTIELNDSLPGDHLITMYNYSAQNVDELSFDKNAILVLIDKNEPEWWMGRNLLSSQEGLFPVNYVRRLQAGNASDEEAMKEVAVASSQYNVNVAGRPVSWYVDGTMTFNAILTEDEKRRQFYIRELIETEKI 1093 HSP 3 Score: 592.808 bits (1527), Expect = 0.000e+0 Identity = 290/292 (99.32%), Postives = 290/292 (99.32%), Query Frame = 3
Query: 4224 VENILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYNTGGMPFVFPLETSSKDKMQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIRRTAGVGRLLVTVVEGISLRXXXXXXXXXXXPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPN 5099
E ILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYNTGGMPFVFPLETSSKDKMQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIRRTAGVGRLLVTVVEGISLRSSSSHKDSKSDPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPN
Sbjct: 1090 TEKILKHTEDNHPDRSDMEEAVAAARSLCESVNEGVRERENSERLEWLQEHMQCENLSEKLYFNSNTNCLGHRKLIHSGTLSKYKSGKELVGFLFNDFFLLATPCYNTGGMPFVFPLETSSKDKMQFKMYKKPIILNQVMISSGSPTSSANSQFSSNDIFTVKEPTCEHILRALSQNDRDKWVERLMNASMFYINTENLKRECSMSIRRTAGVGRLLVTVVEGISLRSSSSHKDSKSDPFCEASLGNQLHRTKVVPSTLNPKWNTQMQFLIKNIDQDVLCLTVYNKDYFAPN 1381 The following BLAST results are available for this feature:
BLAST of Intersectin-1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Intersectin-1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Intersectin-1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Intersectin-1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Intersectin-1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Intersectin-1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Intersectin-1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Intersectin-1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Intersectin-1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Intersectin-1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Intersectin-1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 5
BLAST of Intersectin-1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Intersectin-1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Intersectin-1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30000291 ID=SMED30000291|Name=Intersectin-1|organism=Schmidtea mediterranea sexual|type=transcript|length=5564bpback to top protein sequence of SMED30000291-orf-1 >SMED30000291-orf-1 ID=SMED30000291-orf-1|Name=SMED30000291-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1735bp MLNNPNLWFITSEEKVKFDVQFQCLNPVNGFITGNQAKSLFTKSNLPTDVback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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