X1.A.G7.1
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30000282 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 10
Homology
BLAST of X1.A.G7.1 vs. TrEMBL
Match: V9XRT9 (X1.A.G7.1 (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 70.4774 bits (171), Expect = 9.262e-11 Identity = 64/204 (31.37%), Postives = 97/204 (47.55%), Query Frame = 2 Query: 203 PPPRYD----GLVMNNQVIVVSQPAPEPPQNVVLRFK-----------KAHLKLGILLTIVGGIQMILDPLLPALFMYPYSLFPGGLFMLISGICSIHGSRREDRCSLRASLTFNILALPLVFXXXXXXXXXXXSGTSILYCQGSSYLCYPNMNFYASGLKGFSIFNLIYGII---LFVFLCWDSGIKCGDVCCCKRRQPRVTF 760 PP Y L++N+ I Q P + V+L K K+H G +L G + +DPLLP L YP+S+ G+FM+ISGI SI +R + L + NILAL + I I+ +ILY G+++ Y N G IF + YGI+ L V L ++S + C ++CC ++R +V + Sbjct: 16 SPPEYSQQPTNLLINSTGIHSDQVQDGPVRLVLLESKEIRSGSKFINSKSHRIFGYILIACGIFVLCIDPLLPILLEYPFSISIAGVFMIISGIFSIQAVKRNLKLYLIFAKILNILALINIGIGIL---------VAILY--GTAF--YHRQN-------GILIFTVFYGILTMSLLVLLIFNSALICKEICCRQQRMSQVQY 199
BLAST of X1.A.G7.1 vs. Planmine SMEST
Match: SMESG000046817.1 (SMESG000046817.1) HSP 1 Score: 417.542 bits (1072), Expect = 5.526e-149 Identity = 213/236 (90.25%), Postives = 228/236 (96.61%), Query Frame = 2 Query: 53 MARPNEGFTTDEYATVSEPVPPYPQTFQSPNPPNYNGIIKLPTDVNAIYLPPPRYDGLVMNNQVIVVSQPAPEPPQNVVLRFKKAHLKLGILLTIVGGIQMILDPLLPALFMYPYSLFPGGLFMLISGICSIHGSRREDRCSLRASLTFNILALPLVFXXXXXXXXXXXSGTSILYCQGSSYLCYPNMNFYASGLKGFSIFNLIYGIILFVFLCWDSGIKCGDVCCCKRRQPRVTF 760 MARPNEGFTTDEYATVSEPVPPYPQTFQ PNPPNYN IIK+PTDVN +YLPPPRYDGLVMNNQVIVVSQPAPEPPQNV+LRFKKAHLKLGILLTIVGGIQMILDPLLPALFMYPYSLFPGGLFMLISGICSIHGSRREDRCSLRASLTF+ILAL LVFIAII+AIVV+++G SIL+C+ S YLCYP+++FY+SGLKGFSIFNLIYGIILFV LCWDSGIKCGDVCCCKRRQPRVT+ Sbjct: 1 MARPNEGFTTDEYATVSEPVPPYPQTFQPPNPPNYNDIIKIPTDVNVMYLPPPRYDGLVMNNQVIVVSQPAPEPPQNVILRFKKAHLKLGILLTIVGGIQMILDPLLPALFMYPYSLFPGGLFMLISGICSIHGSRREDRCSLRASLTFDILALVLVFIAIIIAIVVIIAGDSILFCKSSIYLCYPSVDFYSSGLKGFSIFNLIYGIILFVLLCWDSGIKCGDVCCCKRRQPRVTY 236
BLAST of X1.A.G7.1 vs. Planmine SMEST
Match: SMESG000046814.1 (SMESG000046814.1) HSP 1 Score: 417.542 bits (1072), Expect = 5.526e-149 Identity = 213/236 (90.25%), Postives = 228/236 (96.61%), Query Frame = 2 Query: 53 MARPNEGFTTDEYATVSEPVPPYPQTFQSPNPPNYNGIIKLPTDVNAIYLPPPRYDGLVMNNQVIVVSQPAPEPPQNVVLRFKKAHLKLGILLTIVGGIQMILDPLLPALFMYPYSLFPGGLFMLISGICSIHGSRREDRCSLRASLTFNILALPLVFXXXXXXXXXXXSGTSILYCQGSSYLCYPNMNFYASGLKGFSIFNLIYGIILFVFLCWDSGIKCGDVCCCKRRQPRVTF 760 MARPNEGFTTDEYATVSEPVPPYPQTFQ PNPPNYN IIK+PTDVN +YLPPPRYDGLVMNNQVIVVSQPAPEPPQNV+LRFKKAHLKLGILLTIVGGIQMILDPLLPALFMYPYSLFPGGLFMLISGICSIHGSRREDRCSLRASLTF+ILAL LVFIAII+AIVV+++G SIL+C+ S YLCYP+++FY+SGLKGFSIFNLIYGIILFV LCWDSGIKCGDVCCCKRRQPRVT+ Sbjct: 1 MARPNEGFTTDEYATVSEPVPPYPQTFQPPNPPNYNDIIKIPTDVNVMYLPPPRYDGLVMNNQVIVVSQPAPEPPQNVILRFKKAHLKLGILLTIVGGIQMILDPLLPALFMYPYSLFPGGLFMLISGICSIHGSRREDRCSLRASLTFDILALVLVFIAIIIAIVVIIAGDSILFCKSSIYLCYPSVDFYSSGLKGFSIFNLIYGIILFVLLCWDSGIKCGDVCCCKRRQPRVTY 236
BLAST of X1.A.G7.1 vs. Planmine SMEST
Match: SMESG000046812.1 (SMESG000046812.1) HSP 1 Score: 221.476 bits (563), Expect = 8.614e-72 Identity = 109/232 (46.98%), Postives = 161/232 (69.40%), Query Frame = 2 Query: 53 MARPNEGFTTDEYATVSEPVPPYPQTFQSPNPPNYNGIIKLPTDVNAIYLPPPRYDGLVMNNQVIVVSQPAPEPPQNVVLRFKKAHLKLGILLTIVGGIQMILDPLLPALFMYPYSLFPGGLFMLISGICSIHGSRREDRCSLRASLTFNILALPLVFXXXXX-XXXXXXSGTSILYCQGSSYLCYPNMNFYASGLKGFSIFNLIYGIILFVFLCWDSGIKCGDVCCCKRRQ 745 M + + GFTT+E+A + QTF+ +PPNYN I+K P + + +PPP+Y+G IVV E QN +LR+K+ HL G + ++GGIQ+ +DP+LP++F YPYS++PGGLFM+ISG+CSI+GSR+ ++CSL ASL FNILA+ ++F I+ + + SG S C + +CYP+++FY G+KGF+IFN+++GIILFV + WDS +KCGDVC K++Q Sbjct: 1 MEQSDAGFTTEEHAIILNSDRHNLQTFEPLSPPNYNDIVKSPLNGPTLSIPPPQYEG-------IVVPPQTTEESQNSMLRYKRMHLIYGTIYVVLGGIQVAIDPILPSIFQYPYSMYPGGLFMIISGLCSIYGSRKNNKCSLIASLIFNILAMIVIFTNYIIGILALNQSGFSFTPCNQYNSICYPSVSFYTEGVKGFAIFNVVFGIILFVLMIWDSSLKCGDVCFKKQKQ 225
BLAST of X1.A.G7.1 vs. Planmine SMEST
Match: SMESG000046815.1 (SMESG000046815.1) HSP 1 Score: 220.705 bits (561), Expect = 2.340e-71 Identity = 110/232 (47.41%), Postives = 157/232 (67.67%), Query Frame = 2 Query: 53 MARPNEGFTTDEYATVSEPVPPYPQTFQSPNPPNYNGIIKLPTDVNAIYLPPPRYDGLVMNNQVIVVSQPAPEPPQNVVLRFKKAHLKLGILLTIVGGIQMILDPLLPALFMYPYSLFPGGLFMLISGICSIHGSRREDRCSLRASLTFNILALPLVFXXXXX-XXXXXXSGTSILYCQGSSYLCYPNMNFYASGLKGFSIFNLIYGIILFVFLCWDSGIKCGDVCCCKRRQ 745 M + N GFTT+EYA + QTF+ +PPNYN I+K P + +PPP+Y+G I V E QN +LR+K+ HL G + ++GGIQ+ +DP+LP++F YPYS++PGGLFM+ISG+CSI+GSR+ ++CSL ASL FNILAL ++F I+ + SG C + +CYP+++FY G+KGF+IFN+++GIILFV + WDS +KCGDVC K++Q Sbjct: 1 MEQSNAGFTTEEYAIILNSDRHNLQTFEPLSPPNYNDIVKSPLTGPTLSIPPPQYEG-------IAVPPQTTEESQNSMLRYKRMHLIYGTISVVLGGIQVAIDPILPSIFQYPYSMYPGGLFMIISGLCSIYGSRKNNKCSLIASLIFNILALIVIFTNYIIGILASNQSGFFFTPCNQYNAICYPSVSFYTEGVKGFAIFNVVFGIILFVLMIWDSSLKCGDVCFKKQKQ 225
BLAST of X1.A.G7.1 vs. Planmine SMEST
Match: SMESG000046819.1 (SMESG000046819.1) HSP 1 Score: 215.312 bits (547), Expect = 2.907e-69 Identity = 107/232 (46.12%), Postives = 155/232 (66.81%), Query Frame = 2 Query: 53 MARPNEGFTTDEYATVSEPVPPYPQTFQSPNPPNYNGIIKLPTDVNAIYLPPPRYDGLVMNNQVIVVSQPAPEPPQNVVLRFKKAHLKLGILLTIVGGIQMILDPLLPALFMYPYSLFPGGLFMLISGICSIHGSRREDRCSLRASLTFNILALPLVFXXXXX-XXXXXXSGTSILYCQGSSYLCYPNMNFYASGLKGFSIFNLIYGIILFVFLCWDSGIKCGDVCCCKRRQ 745 M + N GFTT+EYA + QTF+ +PPNYN I+K P + +PPP+Y+G I V E QN +LR+K+ HL G + ++GGIQ+ +DP+LP++F YPYS++PGGLFM+ISG+CSI+GSR+ ++CSL ASL FNILAL ++F I+ + SG C + +CYP+++FY G+KG +IFN+++G+ILFV + WDS +KCGDVC ++Q Sbjct: 1 MEQSNAGFTTEEYAIILNSDRHNLQTFEPLSPPNYNDIVKSPLTGPTLSIPPPQYEG-------IAVPPQTTEESQNSMLRYKRMHLIYGTISVVLGGIQVAIDPILPSIFQYPYSMYPGGLFMIISGLCSIYGSRKNNKCSLIASLIFNILALIVIFTNYIIGILASNQSGFFFTPCNQYNAICYPSVSFYTEGVKGLAIFNVVFGMILFVLMIWDSSLKCGDVCFKNQKQ 225 The following BLAST results are available for this feature:
BLAST of X1.A.G7.1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of X1.A.G7.1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of X1.A.G7.1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of X1.A.G7.1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of X1.A.G7.1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of X1.A.G7.1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of X1.A.G7.1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of X1.A.G7.1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 1
BLAST of X1.A.G7.1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of X1.A.G7.1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of X1.A.G7.1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of X1.A.G7.1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of X1.A.G7.1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of X1.A.G7.1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30000282 ID=SMED30000282|Name=X1.A.G7.1|organism=Schmidtea mediterranea sexual|type=transcript|length=875bpback to top protein sequence of SMED30000282-orf-1 >SMED30000282-orf-1 ID=SMED30000282-orf-1|Name=SMED30000282-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=243bp MARPNEGFTTDEYATVSEPVPPYPQTFQSPNPPNYNGIIKLPTDVNAIYLback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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