SMED30000247

Overview
NameSMED30000247
Smed IDSMED30000247
Length (bp)2780
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30000247 (SMED30000247) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30000247 (SMED30000247) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30000247 (SMED30000247) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30000247 (SMED30000247) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30000247

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30000247SMESG000023639.1 SMESG000023635.1 dd_Smed_v4_3360_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30000247SMESG000023639.1 SMESG000023635.1 dd_Smed_v4_3360_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30000247SMESG000023639.1 SMESG000023635.1 dd_Smed_v4_3360_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30000247SMESG000023639.1 SMESG000023635.1 dd_Smed_v4_3360_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30000247 dd_Smed_v4_14688_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30000247 vs. Planmine SMEST
Match: SMESG000054261.1 (SMESG000054261.1)

HSP 1 Score: 142.895 bits (359), Expect = 2.827e-78
Identity = 88/127 (69.29%), Postives = 106/127 (83.46%), Query Frame = 3 
Query:  777 ALLQANLQQDGSAERTELRNAFNE-DRRGSDLEVIHAPVHRFFTHPSSTQITINATGIESPTRISISRVLDPPPIETESLSGRSSPLIISVVEPTITPREEPRRSALNXXXXXXXXXXXXVDIDGDD 1154
            ALLQANL+Q+   + +EL+N F+E DR  SDL+VI+APV  FF HPSSTQIT+N T I+SPTRISIS +L+PPPIETE+ S  SSP+I+SVVEPTI+ +EEPRRSA NPP+RCPKRR LPVDID DD
Sbjct:  160 ALLQANLRQEEPMKMSELQNTFDEEDRCASDLDVIYAPVPPFFQHPSSTQITVNITRIKSPTRISISTMLNPPPIETETTSTHSSPVILSVVEPTISLQEEPRRSAPNPPVRCPKRRRLPVDIDDDD 286

HSP 2 Score: 135.191 bits (339), Expect = 2.827e-78
Identity = 70/92 (76.09%), Postives = 77/92 (83.70%), Query Frame = 2 
Query:  302 MSILPSLPRISKISPSIVAMSHTLDKLERENSILKEKYQKIKGTWSSPETAKGLYQKISRLSRARDDLIIENRSQRITISEQSVALR*IFTE 577
            MS L  LPRI KISPS+ A    LDKLEREN+IL+EKYQ++KGTWSS ET K LYQKISRLSRARDDLIIENRS+RITIS+QS AL  IFTE
Sbjct:    1 MSTLSGLPRIPKISPSVAATLQKLDKLERENAILQEKYQRVKGTWSSTETIKRLYQKISRLSRARDDLIIENRSRRITISKQSAALWQIFTE 92

HSP 3 Score: 56.9954 bits (136), Expect = 2.827e-78
Identity = 29/52 (55.77%), Postives = 33/52 (63.46%), Query Frame = 1 
Query:  568 FHRNELMTRLPSRRLAQELDIIPAHHLP---SKQKPRCLVYFPRPATPGTFW 714
            F  +EL+TRLPSRRL QELDIIPAHHLP   + Q P  L Y   P +    W
Sbjct:   90 FTEHELLTRLPSRRLTQELDIIPAHHLPLQTTIQMPNTLSYTNNPESVSGNW 141
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BLAST of SMED30000247 vs. Planmine SMEST
Match: SMESG000044502.1 (SMESG000044502.1)

HSP 1 Score: 141.739 bits (356), Expect = 3.032e-60
Identity = 72/93 (77.42%), Postives = 79/93 (84.95%), Query Frame = 2 
Query:  302 MSILPSLPRISKISPSIVAMSHTLDKLERENSILKEKYQKIKGTWSSPETAKGLYQKISRLSRARDDLIIENRSQRITISEQSVALR*IFTET 580
            MS L ++PRI +ISPS+VAMS  LDKLE EN+IL+EKYQKIK TW SPET KGLYQKISRLSRAR DLIIENRSQ ITIS+QS ALR IFTE 
Sbjct:    1 MSALSNMPRIPRISPSVVAMSQKLDKLELENAILQEKYQKIKDTWCSPETTKGLYQKISRLSRARGDLIIENRSQWITISKQSTALRQIFTEN 93

HSP 2 Score: 111.309 bits (277), Expect = 3.032e-60
Identity = 81/130 (62.31%), Postives = 97/130 (74.62%), Query Frame = 3 
Query:  642 PFTLQAETKMSSLLPSTSNSGNILEXXXXXXXXXXXXXXXXXXXXALLQANLQQDGSAERTELRNAFNE--DRRGSDLEVIHAPVHRFFTHPSSTQITINATGIESPTRISISRVLDPPPIETESLSGRS 1025
            PF  QAET+M+S  P TSNS N   NR HKPNRNR  RQKRR +NALLQ+NL  +G  E TE R+  NE  +RR S LE+I+APV  FF+HPSS QITIN TGIESPT+++IS++L+PP IE E  SGRS
Sbjct:  117 PF--QAETQMTSQHPLTSNSENASGNRCHKPNRNRGWRQKRRVENALLQSNLGPNGCVEMTEFRDVSNEEKNRRASYLEIIYAPVQPFFSHPSSAQITINTTGIESPTQVNISKMLNPPLIEREPTSGRS 244

HSP 3 Score: 53.9138 bits (128), Expect = 1.702e-7
Identity = 29/64 (45.31%), Postives = 36/64 (56.25%), Query Frame = 1 
Query:  568 FHRNELMTRLPSRRLAQELDIIPAHHLPSKQKPRCLVYFPRPATPGTFWKIGGTSRTETVAGGR 759
            F  NEL+ RLPSR+LAQELDIIPAHHLP + + +     P             TS +E  +G R
Sbjct:   90 FTENELLNRLPSRKLAQELDIIPAHHLPFQAETQMTSQHPL------------TSNSENASGNR 141
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BLAST of SMED30000247 vs. Planmine SMEST
Match: SMESG000025328.1 (SMESG000025328.1)

HSP 1 Score: 133.265 bits (334), Expect = 3.388e-47
Identity = 99/169 (58.58%), Postives = 126/169 (74.56%), Query Frame = 3 
Query:  651 LQAETKMSSLLPSTSNSGNILEXXXXXXXXXXXXXXXXXXXXALLQANLQQDGSAERTELRNAF-NEDRRGSDLEVIHAPVHRFFTHPSSTQITINATGIESPTRISISRVLDPPPIETESLSGRSSPLIISVVEPTITPREEPRRSALNXXXXXXXXXXXXVDIDGDD 1154
            LQ   ++ ++LPST N  +   ++RH+PNRN   RQ+RRA+NALLQANL+ +G  E  EL+  F  EDRR SDL+ ++APV  FF HP ST+I INATG+  PTRISIS +L+PP IETE+ SG SSP+I+SVVEP  + +EEPRRSA NPP+R  KRR  PVDI+GDD
Sbjct:   64 LQPTIQLPNILPSTGNPESRSGSQRHRPNRNSLHRQRRRAENALLQANLRPEGHMEILELQTTFEREDRRASDLDAVYAPVLPFFRHPFSTEININATGLVPPTRISISTMLNPPSIETETSSGHSSPVILSVVEPATSSQEEPRRSAPNPPVRYHKRRRHPVDINGDD 232

HSP 2 Score: 50.0618 bits (118), Expect = 3.388e-47
Identity = 23/28 (82.14%), Postives = 25/28 (89.29%), Query Frame = 1 
Query:  568 FHRNELMTRLPSRRLAQELDIIPAHHLP 651
            F  +EL+T LPSRRLAQELDIIPAHHLP
Sbjct:   36 FTEHELLTSLPSRRLAQELDIIPAHHLP 63

HSP 3 Score: 46.595 bits (109), Expect = 3.388e-47
Identity = 22/28 (78.57%), Postives = 26/28 (92.86%), Query Frame = 2 
Query:  494 RDDLIIENRSQRITISEQSVALR*IFTE 577
            +DDLIIENRS+RITIS+Q+ ALR IFTE
Sbjct:   11 QDDLIIENRSERITISKQNAALRQIFTE 38
[Close]
BLAST of SMED30000247 vs. Planmine SMEST
Match: SMESG000003316.1 (SMESG000003316.1)

HSP 1 Score: 137.502 bits (345), Expect = 2.904e-46
Identity = 68/93 (73.12%), Postives = 79/93 (84.95%), Query Frame = 2 
Query:  299 SMSILPSLPRISKISPSIVAMSHTLDKLERENSILKEKYQKIKGTWSSPETAKGLYQKISRLSRARDDLIIENRSQRITISEQSVALR*IFTE 577
            S+S LP +PRIS  +PS+VAM+  ++KLEREN+ILKEKY K+KGTW SPE  KGLYQKISRLSR  D+LIIENR+QRITISEQS ALR IFTE
Sbjct:    3 SLSQLPRIPRIS-FNPSVVAMTQKIEKLERENAILKEKYHKVKGTWCSPEATKGLYQKISRLSRTHDNLIIENRNQRITISEQSAALRLIFTE 94

HSP 2 Score: 68.9366 bits (167), Expect = 2.904e-46
Identity = 42/98 (42.86%), Postives = 56/98 (57.14%), Query Frame = 1 
Query:  568 FHRNELMTRLPSRRLAQELDIIPAHHLPSKQKPRCL----VYFPRPATPGTFWKIGGTSRTETVAGGRSAGQKMPYCKPIYSRMDLQKGLSYGMRSMR 849
            F   EL+ R+PSR+LAQELDIIPAHHLP++ + +             TPG  W     +RTE    G+ AG KMP    IY+  DL++  S  +RS+R
Sbjct:   92 FTERELLARVPSRKLAQELDIIPAHHLPTQAEAQISGQNHSTGISENTPGNRWHKSNRNRTE----GKDAGPKMPCYWLIYNVTDLRRRRSTEVRSLR 185
[Close]
BLAST of SMED30000247 vs. Planmine SMEST
Match: SMESG000037373.1 (SMESG000037373.1)

HSP 1 Score: 160.614 bits (405), Expect = 4.919e-45
Identity = 113/170 (66.47%), Postives = 129/170 (75.88%), Query Frame = 3 
Query:  651 LQAETKMSSLLPSTSNSGNILEXXXXXXXXXXXXXXXXXXXXALLQANLQQDGSAERTELRNAFN--EDRRGSDLEVIHAPVHRFFTHPSSTQITINATGIESPTRISISRVLDPPPIETESLSGRSSPLIISVVEPTITPREEPRRSALNXXXXXXXXXXXXVDIDGDD 1154
            LQ ET+M+    STSNS N   +   +   NR +RQKRRA+NAL QANL+  GSAE TEL++ FN  EDRR SDLEVI+APV  FF HPSS Q+TIN TGIESPT+ISIS +L+PP IE E  SG SSPLI+SVVEPTITP EEPRRS  NP LRC KRRCL V+IDGDD
Sbjct:   15 LQTETQMTDQHLSTSNSENA--SGNRRHKPNRNQRQKRRAENALQQANLRHYGSAEMTELQDVFNKEEDRRASDLEVIYAPVQPFFLHPSSAQVTINTTGIESPTQISISTMLNPPSIEAEPTSGHSSPLIVSVVEPTITPLEEPRRSTQNPTLRCTKRRCLTVEIDGDD 182
The following BLAST results are available for this feature:
BLAST of SMED30000247 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000247 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000247 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000247 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000247 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000247 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000247 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000247 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000247 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000247 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000247 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000247 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000247 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000247 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000054261.12.827e-7869.29SMESG000054261.1[more]
SMESG000044502.13.032e-6077.42SMESG000044502.1[more]
SMESG000025328.13.388e-4758.58SMESG000025328.1[more]
SMESG000003316.12.904e-4673.12SMESG000003316.1[more]
SMESG000037373.14.919e-4566.47SMESG000037373.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30000247 ID=SMED30000247|Name=SMED30000247|organism=Schmidtea mediterranea sexual|type=transcript|length=2780bp
TTGTGATGCACCCTAACCGCCACATAACATTTTGTCGATAATATGTAAAT
TTAACTGAAAATATTTTGATGTGCACGCATAAATGCTTCTTCAAAATTAT
ATTATAACTAAAATTTAAATAAATTAAATCTATTATAGAACATTTCTTTC
CCCAGCTGCCTTCATATAAATCACATTCAGTTTCTAATTTCTCGCTAGTA
TTCTGAGAAACTTTCTAACTAAGTCGAAGTACACGAAAGCGATTGCAGAA
CCCATCTACATAATCTGCAAAAAATCAGACTATCGTCTACCTGCTTTCTC
AATGTCTATATTACCCAGTCTACCGCGAATTTCCAAAATTTCTCCATCCA
TCGTGGCAATGTCGCATACGCTTGACAAATTAGAACGGGAAAACTCCATC
TTAAAGGAGAAGTACCAGAAGATTAAAGGCACTTGGAGCTCGCCAGAAAC
TGCCAAAGGGCTCTATCAAAAGATATCGCGTCTCTCCCGAGCCCGAGACG
ATTTGATTATAGAAAACCGGAGCCAGCGGATCACCATATCCGAACAGAGC
GTTGCGCTCCGGTAAATTTTCACAGAAACGAACTCATGACTCGCCTGCCG
AGTAGGAGACTGGCTCAGGAGTTAGATATAATTCCCGCCCACCATTTACC
CTCCAAGCAGAAACCAAGATGTCTAGTTTACTTCCCTCGACCAGCAACTC
CGGGAACATTTTGGAAAATCGGCGGCACAAGCCGAACAGAAACCGTCGCC
GGAGGCAGAAGCGCAGGGCAAAAAATGCCCTATTGCAAGCCAATCTACAG
CAGGATGGATCTGCAGAAAGGACTGAGCTACGGAATGCGTTCAATGAGGA
TCGGCGCGGATCGGACCTGGAAGTAATACATGCGCCGGTGCACCGTTTCT
TTACACACCCCTCCTCGACTCAAATTACTATCAACGCGACGGGAATCGAG
TCCCCCACCCGAATTAGCATCTCCAGGGTGCTCGATCCTCCACCAATAGA
AACAGAGTCGCTAAGTGGACGTTCTTCGCCACTTATCATTTCAGTGGTGG
AACCGACAATCACACCACGAGAGGAACCGAGGCGATCTGCTCTAAATCCG
CCTTTGAGGTGCCCCAAACGTCGGTGTCTACCAGTAGACATCGACGGGGA
TGATTGATTTACCCACCTCCTGATTTTGAATTCCTCGACTTTAGTTTGCG
CTTGTTGACTTTTTGACTAAAGCTTACAACTCTGTGTTTGTCATTAATTT
ACTGTGCTGTTGTACTTGCGTTATTAAAATATACATGGTATGTTATCTTA
TTTCGCAAATTAAATATTAATTCATTTAAAAATTTTAATCACGTAGTATT
TTATGTTATTAGTGTAACCCCTAAATTAAATATCATTTAAACCGAATTTG
TAATTGTGTGCTAAATATTTTTTTTGTGAGTTAAAAAGAGTTAGTTTCAA
ACTTAGTGTAATTTATGATAAGGGTTATGTAGACCTTAGTAATAGAATAG
AGTAAATTAATAGGTAACTTATCTCGTATTGTCGACTGGAAAAATAAAAA
TAGTGGTTGAATTCTGTTAACGTCCTCTAATCAGCAGTTAAAATTCATGA
AATGATTAAGGTCATTGATGTTCGATACCATCCCAATCATGTTTACCATT
CCAGCTATAGGAACCAGGTATATTCAGGAATCGAGGGATCATAACATATC
GCGCAAGACCTACTAACGGCCGCTTTGAACCTGAAACGAAAGTTATACGA
GGAGTAGTATTGGTAACAGTGCTCAATTGCTCGAATGGCATTCGGCAGTC
ATACACATTACAAACATGGCTTCAACCATGGGTGTATGATGGTAATCCGT
AAATCGTGTGGTGTAGAGGGAAATTAACCCTAGAATAGAAAGAGGCGAGT
ATTTTTAGCGGAGGTTATGAATCATTATTAATAAAGTTTTCCCAGTACAC
CAACACTAATAAACTACCAGTTCACAAGGAAACTTGTTTCCCAATTTTTA
ACACGACCAGACCTTATAAATTAAGTTTTATTTCAAAATCTTATCAAAAT
TCTCTAGAAATTTGAAAAAATTATTTATAACCAGTTTCGGTATGGTAATT
AGTAAAATAATTAGATTTTGAATAACTGTCAAAGCATGGAAAACGAATTT
TATAAAAACAATAATCTCGGTGCTTACTTTCTGAACCTGGCACTTTAAGA
TAAGCATTAGAACTTCCACATGGTGTGTCATCCTTTTTTTTCCAGAATGT
TTCATTTTTTTTTAGCAATTTATGTCTGAATATGTATATTAAAACTAGGG
ATAGATGGATTAAAAAGATGATGATAATGATAGCGATGATGACATCAACC
TTTTTGTTAATTGCACTTTGATCTGGTATCAATTTGGACCAATCTTTGAA
CCGATCGTCTATTGATGAATTAAGACCCACCCGCAAAGTTTTAAACGCAT
TCTTCATTTCTTCAATACTAATATTCAAAATATTTTTATTATCGTTCAAA
ATACGAGACAAAATCAAAACCGCATAATCAGACGGTGGGCACTGTTTGCA
TGATTCCAATACTTTCTTACATTCTTCATCTCCTTTCTTAATGCAGATAT
CGGTAAAACTGCTACATGATGAGTCAGATAATCGAAATGGATACTCACTA
ACACAGTAAGACACTGGTTGCTCTTCATAAGCTGGTTTAATTAACAAAGT
AAATAAAACGAAATATAATTTAAATAATATCATGAATAAAGGTTATGGAT
GTATTTTAATACTTTATTCAAACACTTTAC
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protein sequence of SMED30000247-orf-1

>SMED30000247-orf-1 ID=SMED30000247-orf-1|Name=SMED30000247-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=163bp
MSSLLPSTSNSGNILENRRHKPNRNRRRRQKRRAKNALLQANLQQDGSAE
RTELRNAFNEDRRGSDLEVIHAPVHRFFTHPSSTQITINATGIESPTRIS
ISRVLDPPPIETESLSGRSSPLIISVVEPTITPREEPRRSALNPPLRCPK
RRCLPVDIDGDD*
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protein sequence of SMED30000247-orf-2

>SMED30000247-orf-2 ID=SMED30000247-orf-2|Name=SMED30000247-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=221bp
MILFKLYFVLFTLLIKPAYEEQPVSYCVSEYPFRLSDSSCSSFTDICIKK
GDEECKKVLESCKQCPPSDYAVLILSRILNDNKNILNISIEEMKNAFKTL
RVGLNSSIDDRFKDWSKLIPDQSAINKKVDVIIAIIIIIFLIHLSLVLIY
IFRHKLLKKNETFWKKKDDTPCGSSNAYLKVPGSESKHRDYCFYKIRFPC
FDSYSKSNYFTNYHTETGYK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000101muscle cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..20
score: 0.76
NoneNo IPR availableTMHMMTMhelixcoord: 130..152
NoneNo IPR availableTMHMMTMhelixcoord: 190..212