SMED30000195

Overview
NameSMED30000195
Smed IDSMED30000195
Length (bp)1061
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30000195 (SMED30000195) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30000195 (SMED30000195) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30000195 (SMED30000195) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30000195 (SMED30000195) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30000195

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
non-ciliated neuronSMED30000195SMESG000028898.1 dd_Smed_v4_11270_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30000195 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000384:48281..49341 -5251
Homology
[Close]
BLAST of SMED30000195 vs. Ensembl Human
Match: STX17 (syntaxin 17 [Source:HGNC Symbol;Acc:HGNC:11432])

HSP 1 Score: 53.5286 bits (127), Expect = 2.076e-7
Identity = 59/258 (22.87%), Postives = 116/258 (44.96%), Query Frame = 3 
Query:  147 NLYAYEERITYFINTFMNLSLE-IKSHKISLQNS-----YEKINESQMNNVIYDASITIKSSEILYNDLSEFRKFIPFHLKIDFDRFII-KYKVNFEKEKTLYVQMENHISSLLKNMSSIHNNSNTDVNLDQIELIDDIC-----HFQPDNMYSESNSNI-LLPDF-------ENLDKIEDKINVVNDMFIEMSSLIETTKSTVDNTFKNVDAANQMVTNATSNIISASKYKAALIPVGTSLMGALICGPVGFCIGLK 860
             L   E  I  FI   +   LE ++ H+I+++       ++K++E  +N     A  T++    L +++ E  K     LK+  D  ++ K  ++  KE+      E  +   L+++  +    N +  L Q  L   +      H       S+S + I  LP+        E+ + +E  +  ++ +  + S L+ + +  +D+   +V++A   V   T N+  A+KYK A +PV  +L+G ++ GP+G   G K
Sbjct:    9 KLRRLEPAIQKFIKIVIPTDLERLRKHQINIEKYQRCRIWDKLHEEHIN-----AGRTVQQ---LRSNIREIEKLC---LKVRKDDLVLLKRMIDPVKEEASAATAE-FLQLHLESVEELKKQFNDEETLLQPPLTRSMTVGGAFHTTEAEASSQSLTQIYALPEIPQDQNAAESWETLEADLIELSQLVTDFSLLVNSQQEKIDSIADHVNSAAVNVEEGTKNLGKAAKYKLAALPVAGALIGGMVGGPIGLLAGFK 254
[Close]
BLAST of SMED30000195 vs. Ensembl Human
Match: STX17 (syntaxin 17 [Source:HGNC Symbol;Acc:HGNC:11432])

HSP 1 Score: 53.5286 bits (127), Expect = 2.076e-7
Identity = 59/258 (22.87%), Postives = 116/258 (44.96%), Query Frame = 3 
Query:  147 NLYAYEERITYFINTFMNLSLE-IKSHKISLQNS-----YEKINESQMNNVIYDASITIKSSEILYNDLSEFRKFIPFHLKIDFDRFII-KYKVNFEKEKTLYVQMENHISSLLKNMSSIHNNSNTDVNLDQIELIDDIC-----HFQPDNMYSESNSNI-LLPDF-------ENLDKIEDKINVVNDMFIEMSSLIETTKSTVDNTFKNVDAANQMVTNATSNIISASKYKAALIPVGTSLMGALICGPVGFCIGLK 860
             L   E  I  FI   +   LE ++ H+I+++       ++K++E  +N     A  T++    L +++ E  K     LK+  D  ++ K  ++  KE+      E  +   L+++  +    N +  L Q  L   +      H       S+S + I  LP+        E+ + +E  +  ++ +  + S L+ + +  +D+   +V++A   V   T N+  A+KYK A +PV  +L+G ++ GP+G   G K
Sbjct:    9 KLRRLEPAIQKFIKIVIPTDLERLRKHQINIEKYQRCRIWDKLHEEHIN-----AGRTVQQ---LRSNIREIEKLC---LKVRKDDLVLLKRMIDPVKEEASAATAE-FLQLHLESVEELKKQFNDEETLLQPPLTRSMTVGGAFHTTEAEASSQSLTQIYALPEIPQDQNAAESWETLEADLIELSQLVTDFSLLVNSQQEKIDSIADHVNSAAVNVEEGTKNLGKAAKYKLAALPVAGALIGGMVGGPIGLLAGFK 254
[Close]
BLAST of SMED30000195 vs. Ensembl Human
Match: STX17 (syntaxin 17 [Source:HGNC Symbol;Acc:HGNC:11432])

HSP 1 Score: 53.5286 bits (127), Expect = 2.076e-7
Identity = 59/258 (22.87%), Postives = 116/258 (44.96%), Query Frame = 3 
Query:  147 NLYAYEERITYFINTFMNLSLE-IKSHKISLQNS-----YEKINESQMNNVIYDASITIKSSEILYNDLSEFRKFIPFHLKIDFDRFII-KYKVNFEKEKTLYVQMENHISSLLKNMSSIHNNSNTDVNLDQIELIDDIC-----HFQPDNMYSESNSNI-LLPDF-------ENLDKIEDKINVVNDMFIEMSSLIETTKSTVDNTFKNVDAANQMVTNATSNIISASKYKAALIPVGTSLMGALICGPVGFCIGLK 860
             L   E  I  FI   +   LE ++ H+I+++       ++K++E  +N     A  T++    L +++ E  K     LK+  D  ++ K  ++  KE+      E  +   L+++  +    N +  L Q  L   +      H       S+S + I  LP+        E+ + +E  +  ++ +  + S L+ + +  +D+   +V++A   V   T N+  A+KYK A +PV  +L+G ++ GP+G   G K
Sbjct:    9 KLRRLEPAIQKFIKIVIPTDLERLRKHQINIEKYQRCRIWDKLHEEHIN-----AGRTVQQ---LRSNIREIEKLC---LKVRKDDLVLLKRMIDPVKEEASAATAE-FLQLHLESVEELKKQFNDEETLLQPPLTRSMTVGGAFHTTEAEASSQSLTQIYALPEIPQDQNAAESWETLEADLIELSQLVTDFSLLVNSQQEKIDSIADHVNSAAVNVEEGTKNLGKAAKYKLAALPVAGALIGGMVGGPIGLLAGFK 254
[Close]
BLAST of SMED30000195 vs. Ensembl Fly
Match: Syx17 (gene:FBgn0035540 transcript:FBtr0073293)

HSP 1 Score: 55.4546 bits (132), Expect = 1.995e-8
Identity = 28/107 (26.17%), Postives = 50/107 (46.73%), Query Frame = 3 
Query:  603 FENLDKIEDKINVVNDMFIEMSSLIETTKSTVDNTFKNVDAANQMVTNATSNIISASKYKAALIPVGTSLMGALICGPVGFCIGLKTGLVTGVAGAMFGGVCGKIVK 923
             + ++ ++ +I  ++ MF  M  L       V+    N + A + V     N+  A  YK A+ PV  +L+G  + GP+G   G+K G +  V   + G   G ++K
Sbjct:  163 LDQMENLQQEIYDLHGMFQGMRQLTAEQSVAVEKIADNAEEALENVQQGELNLRRALTYKKAMYPVVGALLGTCVGGPIGLVAGMKAGGLAAVGCGILGFTGGSVLK 269
[Close]
BLAST of SMED30000195 vs. Ensembl Fly
Match: Syx17 (gene:FBgn0035540 transcript:FBtr0330179)

HSP 1 Score: 54.6842 bits (130), Expect = 4.404e-8
Identity = 28/107 (26.17%), Postives = 50/107 (46.73%), Query Frame = 3 
Query:  603 FENLDKIEDKINVVNDMFIEMSSLIETTKSTVDNTFKNVDAANQMVTNATSNIISASKYKAALIPVGTSLMGALICGPVGFCIGLKTGLVTGVAGAMFGGVCGKIVK 923
             + ++ ++ +I  ++ MF  M  L       V+    N + A + V     N+  A  YK A+ PV  +L+G  + GP+G   G+K G +  V   + G   G ++K
Sbjct:  163 LDQMENLQQEIYDLHGMFQGMRQLTAEQSVAVEKIADNAEEALENVQQGELNLRRALTYKKAMYPVVGALLGTCVGGPIGLVAGMKAGGLAAVGCGILGFTGGSVLK 269
[Close]
BLAST of SMED30000195 vs. UniProt/SwissProt
Match: sp|P56962|STX17_HUMAN (Syntaxin-17 OS=Homo sapiens OX=9606 GN=STX17 PE=1 SV=2)

HSP 1 Score: 53.5286 bits (127), Expect = 9.969e-7
Identity = 59/258 (22.87%), Postives = 116/258 (44.96%), Query Frame = 3 
Query:  147 NLYAYEERITYFINTFMNLSLE-IKSHKISLQNS-----YEKINESQMNNVIYDASITIKSSEILYNDLSEFRKFIPFHLKIDFDRFII-KYKVNFEKEKTLYVQMENHISSLLKNMSSIHNNSNTDVNLDQIELIDDIC-----HFQPDNMYSESNSNI-LLPDF-------ENLDKIEDKINVVNDMFIEMSSLIETTKSTVDNTFKNVDAANQMVTNATSNIISASKYKAALIPVGTSLMGALICGPVGFCIGLK 860
             L   E  I  FI   +   LE ++ H+I+++       ++K++E  +N     A  T++    L +++ E  K     LK+  D  ++ K  ++  KE+      E  +   L+++  +    N +  L Q  L   +      H       S+S + I  LP+        E+ + +E  +  ++ +  + S L+ + +  +D+   +V++A   V   T N+  A+KYK A +PV  +L+G ++ GP+G   G K
Sbjct:    9 KLRRLEPAIQKFIKIVIPTDLERLRKHQINIEKYQRCRIWDKLHEEHIN-----AGRTVQQ---LRSNIREIEKLC---LKVRKDDLVLLKRMIDPVKEEASAATAE-FLQLHLESVEELKKQFNDEETLLQPPLTRSMTVGGAFHTTEAEASSQSLTQIYALPEIPQDQNAAESWETLEADLIELSQLVTDFSLLVNSQQEKIDSIADHVNSAAVNVEEGTKNLGKAAKYKLAALPVAGALIGGMVGGPIGLLAGFK 254
[Close]
BLAST of SMED30000195 vs. TrEMBL
Match: E9FW19 (t-SNARE coiled-coil homology domain-containing protein OS=Daphnia pulex OX=6669 GN=DAPPUDRAFT_220863 PE=4 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 2.843e-13
Identity = 42/115 (36.52%), Postives = 65/115 (56.52%), Query Frame = 3 
Query:  588 ILLPDFENLDKIEDKINVVNDMFIEMSSLIETTKSTVDNTFKNVDAANQMVTNATSNIISASKYKAALIPVGTSLMGALICGPVGFCIGLKTGLVTGVAGAMFGGVCGKIVKQFQ 932
            I L  +E+L K    I  ++++F E +  + T  STV N   NVD A + V     N+  A+K+KA + PV  +L+G  I GPVGF  GLK G +  + G++ G   G+ +K++Q
Sbjct:  145 IQLETYEDLQK---DIEELHNLFSEFAQQVHTQGSTVSNIESNVDDALENVKEGQQNLAKAAKFKATMYPVTGALIGTCIGGPVGFLAGLKIGGLAALGGSLLGFASGRGLKKWQ 256
[Close]
BLAST of SMED30000195 vs. TrEMBL
Match: A0A3Q0JLC9 (syntaxin-17 OS=Diaphorina citri OX=121845 GN=LOC103521454 PE=4 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 3.153e-13
Identity = 60/226 (26.55%), Postives = 109/226 (48.23%), Query Frame = 3 
Query:  240 NSYEKINESQMNNVIYDASITIKSSEILYNDLSEFRKFIPFHLKIDFDRF------IIKYKVNFEKEKTLYVQMENHISSLLKNMSSIHNNSNTDVNLDQIELIDDICHFQPDNMYSESNSNILLPDFENLDKIEDKINVVNDMFIEMSSLIETTKSTVDNTFKNVDAANQMVTNATSNIISASKYKAALIPVGTSLMGALICGPVGFCIGLKTGLVTGVAGAMFG 899
            N++  IN+ Q+N     AS T+K       DL E    +  H   D D+F      I K  +N  KE   Y  +EN I  + +N +S  ++    +  D+ E ++++     +    + N  I +   E ++++E+ I+ VN++F +++ ++     TV     N++ A++ V    S +  A+KYKA + P+  ++ G  + GP+G   G K G V  V G + G
Sbjct:   44 NNWHAINQEQIN-----ASRTVKQLNATLKDLREIEAKVQRH---DVDKFKRSIADIKKDALNVLKE---YQDLENKIKLVKENETSKSDDERKRME-DEEEAMNELSRQMLE--IKQDNEQIQMVAAE-MERLENNIHEVNEIFTDLAQIVHAQGETVSTIEDNIETAHENVIAGESQLAKAAKYKAGMYPLIGAVAGICVGGPIGALAGFKMGSVAAVTGTIIG 254
[Close]
BLAST of SMED30000195 vs. TrEMBL
Match: A0A162STE0 (Syntaxin-17 OS=Daphnia magna OX=35525 GN=APZ42_011596 PE=4 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 1.447e-12
Identity = 39/120 (32.50%), Postives = 66/120 (55.00%), Query Frame = 3 
Query:  603 FENLDKIEDKINVVNDMFIEMSSLIETTKSTVDNTFKNVDAANQMVTNATSNIISASKYKAALIPVGTSLMGALICGPVGFCIGLKTGLVTGVAGAMFGGVCGKIVKQFQ---INEISNS 953
            FE    ++  I  ++D+F E S  + T ++ V N  +NV+AA + V     N+  A+++K  L P+  +L+G  I GP+G   GLK G +  + G   G   G+ +K++Q   +NE +NS
Sbjct:  150 FETYQNLQKDIEELHDLFSEFSHQVNTQETAVSNIEENVEAALEDVKEGQHNLAKAARFKTTLYPLTGALIGTCIGGPIGLLAGLKIGGLAALGGTALGFASGRGLKRWQESAVNESANS 269
[Close]
BLAST of SMED30000195 vs. TrEMBL
Match: A0A401S3V0 (t-SNARE coiled-coil homology domain-containing protein OS=Chiloscyllium punctatum OX=137246 GN=chiPu_0003456 PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 8.561e-11
Identity = 37/116 (31.90%), Postives = 65/116 (56.03%), Query Frame = 3 
Query:  585 NILLPDF-------ENLDKIEDKINVVNDMFIEMSSLIETTKSTVDNTFKNVDAANQMVTNATSNIISASKYKAALIPVGTSLMGALICGPVGFCIGLKTGLVTGVAGAMFGGVCG 911
            N+ LP         E+ +K+E+ +  ++++  E S L+ T +  +D+   +V  A + V   T N+  A+KYK A++PV  +L+G ++ GP+G   G K   + GVA A+ GG+ G
Sbjct:  160 NVPLPQIPQDQDAAESWEKLEEDLIDLSNLVNEFSQLLHTQQEKIDSIEDHVSTAAENVEEGTKNLAKAAKYKLAMLPVAGALIGGVMGGPIGLLAGFK---IVGVAAAVSGGILG 272
[Close]
BLAST of SMED30000195 vs. TrEMBL
Match: A0A4S2LB00 (Syntaxin-17 OS=Temnothorax longispinosus OX=300112 GN=DBV15_08320 PE=4 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.285e-10
Identity = 34/119 (28.57%), Postives = 63/119 (52.94%), Query Frame = 3 
Query:  615 DKIEDKINVVNDMFIEMSSLIETTKSTVDNTFKNVDAANQMVTNATSNIISASKYKAALIPVGTSLMGALICGPVGFCIGLKTGLVTGVAGAMFGGVCGKIVKQFQINEISNSEKCLNK 971
            ++++ +I  ++++F+E + ++   K  VDN   N++     V      +  AS+YK A  P+  +++G  I GP+G   GLK G +  V   + G   G ++K+ Q+ E    E CL+K
Sbjct:  162 NRLQGEIQQLHELFVEFNKVVHDQKEMVDNMEDNIEETQINVNEGAKYLQKASRYKVAAYPLAGAMLGTCIGGPIGLLAGLKIGGLAAVGCGILGFTGGSLLKKKQV-ESRKQEPCLDK 279
[Close]
BLAST of SMED30000195 vs. Ensembl Sea Lamprey
Match: stx17 (syntaxin 17 [Source:ZFIN;Acc:ZDB-GENE-041114-164])

HSP 1 Score: 46.9802 bits (110), Expect = 3.175e-6
Identity = 19/85 (22.35%), Postives = 46/85 (54.12%), Query Frame = 3 
Query:  606 ENLDKIEDKINVVNDMFIEMSSLIETTKSTVDNTFKNVDAANQMVTNATSNIISASKYKAALIPVGTSLMGALICGPVGFCIGLK 860
            E+ + + +++  +N +  + S ++ + +  +D+    ++ A+  V   T  I  A+K    ++PV  +++G ++ GPVG  +G K
Sbjct:  179 ESWEHLCEELTELNSLVTDFSYMVHSQQEAIDSIEDKLEMASDNVRQGTKQIGKAAKSGTVMLPVAGAVLGGILGGPVGMLVGFK 263
[Close]
BLAST of SMED30000195 vs. Planmine SMEST
Match: SMESG000028898.1 (SMESG000028898.1)

HSP 1 Score: 571.237 bits (1471), Expect = 0.000e+0
Identity = 282/284 (99.30%), Postives = 283/284 (99.65%), Query Frame = 3 
Query:  126 MISEIPQNLYAYEERITYFINTFMNLSLEIKSHKISLQNSYEKINESQMNNVIYDASITIKSSEILYNDLSEFRKFIPFHLKIDFDRFIIKYKVNFEKEKTLYVQMENHISSLLKNMSSIHNNSNTDVNLDQIELIDDICHFQPDNMYSESNSNILLPDFENLDKIEDKINVVNDMFIEMSSLIETTKSTVDNTFKNVDAANQMVTNATSNIISASKYKAALIPVGTSLMGALICGPVGFCIGLKTGLVTGVAGAMFGGVCGKIVKQFQINEISNSEKCLNKLK 977
            MISEIPQNLYAYEERITYF+N FMNLSLEIKSHKISLQNSYEKINESQMNNVIYDASITIKSSEILYNDLSEFRKFIPFHLKIDFDRFIIKYKVNFEKEKTLYVQMENHISSLLKNMSSIHNNSNTDVNLDQIELIDDICHFQPDNMYSESNSNILLPDFENLDKIEDKINVVNDMFIEMSSLIETTKSTVDNTFKNVDAANQMVTNATSNIISASKYKAALIPVGTSLMGALICGPVGFCIGLKTGLVTGVAGAMFGGVCGKIVKQFQINEISNSEKCLNKLK
Sbjct:    1 MISEIPQNLYAYEERITYFVNIFMNLSLEIKSHKISLQNSYEKINESQMNNVIYDASITIKSSEILYNDLSEFRKFIPFHLKIDFDRFIIKYKVNFEKEKTLYVQMENHISSLLKNMSSIHNNSNTDVNLDQIELIDDICHFQPDNMYSESNSNILLPDFENLDKIEDKINVVNDMFIEMSSLIETTKSTVDNTFKNVDAANQMVTNATSNIISASKYKAALIPVGTSLMGALICGPVGFCIGLKTGLVTGVAGAMFGGVCGKIVKQFQINEISNSEKCLNKLK 284
[Close]
BLAST of SMED30000195 vs. Planmine SMEST
Match: SMESG000028898.1 (SMESG000028898.1)

HSP 1 Score: 531.946 bits (1369), Expect = 0.000e+0
Identity = 266/284 (93.66%), Postives = 268/284 (94.37%), Query Frame = 3 
Query:  126 MISEIPQNLYAYEERITYFINTFMNLSLEIKSHKISLQNSYEKINESQMNNVIYDASITIKSSEILYNDLSEFRKFIPFHLKIDFDRFIIKYKVNFEKEKTLYVQMENHISSLLKNMSSIHNNSNTDVNLDQIELIDDICHFQPDNMYSESNSNILLPDFENLDKIEDKINVVNDMFIEMSSLIETTKSTVDNTFKNVDAANQMVTNATSNIISASKYKAALIPVGTSLMGALICGPVGFCIGLKTGLVTGVAGAMFGGVCGKIVKQFQINEISNSEKCLNKLK 977
            MISEIPQNLYAYEERITYF+N FMNLSLEIKSHKISLQNSYEKINESQMNNVIYDASITIKSSEILYNDLSEFRKFIPFHLKIDFDRFIIKYKVNFEKEKTLYVQMENHISSLLKNMSSIHNNSNTDVNLDQIELIDDICHFQPDNMYSESNSNILLPDFENLDKIEDKINVVNDMFIEMSSLIETTKSTVDNTFKNVDAANQMVTNAT               +GTSLMGALICGPVGFCIGLKTGLVTGVAGAMFGGVCGKIVKQFQINEISNSEKCLNKLK
Sbjct:    1 MISEIPQNLYAYEERITYFVNIFMNLSLEIKSHKISLQNSYEKINESQMNNVIYDASITIKSSEILYNDLSEFRKFIPFHLKIDFDRFIIKYKVNFEKEKTLYVQMENHISSLLKNMSSIHNNSNTDVNLDQIELIDDICHFQPDNMYSESNSNILLPDFENLDKIEDKINVVNDMFIEMSSLIETTKSTVDNTFKNVDAANQMVTNAT---------------IGTSLMGALICGPVGFCIGLKTGLVTGVAGAMFGGVCGKIVKQFQINEISNSEKCLNKLK 269
The following BLAST results are available for this feature:
BLAST of SMED30000195 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
STX172.076e-722.87syntaxin 17 [Source:HGNC Symbol;Acc:HGNC:11432][more]
STX172.076e-722.87syntaxin 17 [Source:HGNC Symbol;Acc:HGNC:11432][more]
STX172.076e-722.87syntaxin 17 [Source:HGNC Symbol;Acc:HGNC:11432][more]
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BLAST of SMED30000195 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000195 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Syx171.995e-826.17gene:FBgn0035540 transcript:FBtr0073293[more]
Syx174.404e-826.17gene:FBgn0035540 transcript:FBtr0330179[more]
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BLAST of SMED30000195 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000195 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000195 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000195 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1
Match NameE-valueIdentityDescription
sp|P56962|STX17_HUMAN9.969e-722.87Syntaxin-17 OS=Homo sapiens OX=9606 GN=STX17 PE=1 ... [more]
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BLAST of SMED30000195 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
E9FW192.843e-1336.52t-SNARE coiled-coil homology domain-containing pro... [more]
A0A3Q0JLC93.153e-1326.55syntaxin-17 OS=Diaphorina citri OX=121845 GN=LOC10... [more]
A0A162STE01.447e-1232.50Syntaxin-17 OS=Daphnia magna OX=35525 GN=APZ42_011... [more]
A0A401S3V08.561e-1131.90t-SNARE coiled-coil homology domain-containing pro... [more]
A0A4S2LB001.285e-1028.57Syntaxin-17 OS=Temnothorax longispinosus OX=300112... [more]
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BLAST of SMED30000195 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000195 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
stx173.175e-622.35syntaxin 17 [Source:ZFIN;Acc:ZDB-GENE-041114-164][more]
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BLAST of SMED30000195 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000195 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000195 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000195 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000028898.10.000e+099.30SMESG000028898.1[more]
SMESG000028898.10.000e+093.66SMESG000028898.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30000195 ID=SMED30000195|Name=SMED30000195|organism=Schmidtea mediterranea sexual|type=transcript|length=1061bp
TTCAAATGTTTTAAATATCTAATTTAAAAAAATATTTATTTTACAATTTA
TTATTACAATTGTACTATTTCATTTGATTAATTTTCCAGTTTTCGTGTAT
TTCTGATTTTCTAAATTATTTTATCATGATTTCAGAAATTCCTCAAAATT
TGTATGCATATGAAGAGCGTATCACATATTTTATAAACACTTTTATGAAT
TTATCACTAGAAATTAAATCTCATAAAATTAGCTTACAAAATTCATATGA
AAAAATAAATGAAAGTCAAATGAACAATGTCATTTACGATGCCTCAATTA
CTATTAAATCATCTGAAATTTTGTATAATGATTTGAGTGAATTTCGCAAA
TTTATTCCATTTCATCTAAAAATTGATTTTGACAGATTTATAATTAAATA
TAAAGTTAATTTCGAAAAAGAAAAAACTTTGTATGTACAGATGGAGAACC
ATATCAGTTCACTTTTAAAAAATATGTCTTCTATTCATAATAATTCTAAT
ACTGATGTGAATCTTGACCAAATTGAATTAATTGATGACATTTGTCATTT
TCAACCAGACAATATGTATTCAGAATCAAATTCTAACATTTTACTTCCTG
ACTTTGAAAATTTAGACAAGATAGAAGATAAAATTAATGTTGTTAATGAT
ATGTTTATTGAAATGTCATCTTTGATCGAAACTACAAAATCTACAGTGGA
TAATACATTTAAAAATGTTGATGCAGCTAATCAAATGGTAACTAATGCAA
CGAGTAATATTATCAGTGCTTCTAAATACAAAGCAGCTTTAATTCCAGTT
GGAACATCTTTAATGGGTGCTTTAATTTGTGGCCCGGTAGGATTTTGTAT
TGGACTAAAAACTGGTTTGGTTACCGGTGTTGCTGGAGCCATGTTTGGAG
GAGTTTGCGGAAAAATTGTCAAACAATTTCAGATTAATGAAATATCGAAT
TCAGAAAAATGTTTAAATAAATTAAAATAATACATTTGTAGTTGTTGCAT
TTAAACAAGGTATTATTGGTAATGTAAAATAAATAAAACTTTATTATCGA
CTAATTACAGC
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protein sequence of SMED30000195-orf-1

>SMED30000195-orf-1 ID=SMED30000195-orf-1|Name=SMED30000195-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=285bp
MISEIPQNLYAYEERITYFINTFMNLSLEIKSHKISLQNSYEKINESQMN
NVIYDASITIKSSEILYNDLSEFRKFIPFHLKIDFDRFIIKYKVNFEKEK
TLYVQMENHISSLLKNMSSIHNNSNTDVNLDQIELIDDICHFQPDNMYSE
SNSNILLPDFENLDKIEDKINVVNDMFIEMSSLIETTKSTVDNTFKNVDA
ANQMVTNATSNIISASKYKAALIPVGTSLMGALICGPVGFCIGLKTGLVT
GVAGAMFGGVCGKIVKQFQINEISNSEKCLNKLK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: INTERPRO
TermDefinition
IPR000727T_SNARE_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0000149SNARE binding
Vocabulary: cellular component
TermDefinition
GO:0005737cytoplasm
GO:0016020membrane
Vocabulary: biological process
TermDefinition
GO:0006888ER to Golgi vesicle-mediated transport
GO:0016192vesicle-mediated transport
GO:0097111endoplasmic reticulum-Golgi intermediate compartment organization
GO:0097352autophagosome maturation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.20.5.110coord: 160..222
e-value: 1.2E-8
score: 36.8
NoneNo IPR availableSUPERFAMILYSSF58038SNARE fusion complexcoord: 161..219
IPR000727Target SNARE coiled-coil homology domainPROSITEPS50192T_SNAREcoord: 153..215
score: 10.141