Netrin 1
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30000153 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 8
Homology
BLAST of Netrin 1 vs. Ensembl Human
Match: NTN1 (netrin 1 [Source:HGNC Symbol;Acc:HGNC:8029]) HSP 1 Score: 312.768 bits (800), Expect = 1.742e-97 Identity = 193/590 (32.71%), Postives = 307/590 (52.03%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGK---CLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSL--LPNSRLIKEWVSVTDIMIS---LLTDGSKKRNKSPYDHLS----ISDITIGARSQCYGHAKSLIK-KNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPC------APETKNNLMATEERCGS---CGKNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRIQQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKCDR 1837
C E+G + C PDF+N A V+STCG+ + R C S E + C C+ P+ + + + D N + T W S +F H++ +T++L K +EV Y+ ++F P+SM +YK G+TW+P+ ++S+ C+K + P+ Q N+ C ++ ++ + + F+ + GR S NS ++++WV+ TDI ++ L T G + + S S +SD+ +G R +C GHA ++ ++ + VCDC H TAGP+C +C H D+PW+ E N C C CN HA C+F+ ++YK S K G CL+C H+T G HC+ C GYYR+ PI H + C+ C CHP G+ ++ CN G C CK GV G CNRC KGYQQ++S PC P T + + E C S K + +I +K+CKK ++I I + G+W +F V ++ +++ S + + S +W+ + DI CKCP ++ YLLL ++ +Q ++ D+ S + W D W RRL F+ RE++GKC +
Sbjct: 41 CSDENGHPRRCIPDFVNAAFGKDVRVSSTCGRPPA--RYC----VVSERGEERLRSCHLCNASDPKKAHPPAFLTDLNNPHNLTCWQSENYLQFP--HNVTLTLSLGKKFEVTYVSLQFCSPRPESMAIYKSMDYGRTWVPFQFYSTQCRKMYNRPHRAPITKQ----NEQEAVCTDSHTDMRPLSGGL--IAFSTLDGRPSAHDFDNSPVLQDWVTATDIRVAFSRLHTFGDENEDDSELARDSYFYAVSDLQVGGRCKCNGHAARCVRDRDDSLVCDCRHNTAGPECDRCKPFHYDRPWQRATARE--ANECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGYYRDMGKPITHRKACKACDCHPVGAAGKT-CNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCIKIPVAPPTTAASSVEEPEDCDSYCKASKGKLKINMKKYCKKDYAVQIHILKADKAGDWWKFTVNIISVYKQGTS-----RIRRGDQS---LWIRSRDIACKCPKIKPLKKYLLLGNA--EDSPDQSGIVADKSSLVIQWRDTWARRLRKFQQREKKGKCKK 603
BLAST of Netrin 1 vs. Ensembl Human
Match: NTN3 (netrin 3 [Source:HGNC Symbol;Acc:HGNC:8030]) HSP 1 Score: 240.35 bits (612), Expect = 2.739e-70 Identity = 161/571 (28.20%), Postives = 253/571 (44.31%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSV-QNITSNIKVLPFNPMTGRTSLLPNSRLIKEWVSVTDIMISLL---TDGSKKRNKS--PYDHLSISDITIGARSQCYGHA-KSLIKKNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPCAP-----ETKNNLMATEERCGS-CGKNRP--RIRFRKFCKKHIMIKISIQSK-EIHGEWTRFGVRVLKIWRIQQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTY----LLLTKIKLKNYLNQPELIVDRKSSALDWSDDWI 1786
C E G + C P +N A +STCG+ + CD P + + +L+ W S +P+ ++ +T+ L K +E+ ++ + F P S+ L K +G++W P +FSS C + L P F + Q S + + L +S ++++WV+ TD+ + L T G + ++ PY + + +D+ +G R +C GHA + L+ G +CDC H T GPDC +C + D+PW+ E + C C CN HA C+F+ ++Y+ S + G CL+C H+T G HC+ C G+YR+ ++ + C+ C CHP G+ ++ CN G C CK GV G CNRC G+QQ++S PC T+++ + C S C R RI +KFCKK +++++ ++ E G WTRF V VL ++R + + +WV D C CP L G Y P LI R S L W D W
Sbjct: 30 CHDEGGAPRGCVPGLVNAALGREVLASSTCGRPATR-------------------ACDASDPRRAHSPALLTSPGGTASPLCWRSESLPR--APLNVTLTVPLGKAFELVFVSLRFCSAPPASVALLKSQDHGRSWAPLGFFSSHCDLDYGR-----LPAPANGPAGPGPEALCFPAPLAQPDGSGLLAFSMQDSSPPGLDLDSSPVLQDWVTATDVRVVLTRPSTAGDPRDMEAVVPYSY-AATDLQVGGRCKCNGHASRCLLDTQGHLICDCRHGTEGPDCGRCKPFYCDRPWQRATARE--SHACLACSCNGHARRCRFNMELYRLSGRRSGGVCLNCRHNTAGRHCHYCREGFYRDPGRALSDRRACRACDCHPVGAAGKT-CNQTTGQCPCKDGVTGLTCNRCAPGFQQSRSPVAPCVKTPIPGPTEDSSPVQPQDCDSHCKPARGSYRISLKKFCKKDYAVQVAVGARGEARGAWTRFPVAVLAVFRSGEE--------RARRGSSALWVPAGDAACGCPRLLPGRRYLLLGGGPGAAAGGAGGRGPGLIAARGSLVLPWRDAWT 562
BLAST of Netrin 1 vs. Ensembl Human
Match: NTN5 (netrin 5 [Source:HGNC Symbol;Acc:HGNC:25208]) HSP 1 Score: 196.052 bits (497), Expect = 9.030e-55 Identity = 116/339 (34.22%), Postives = 160/339 (47.20%), Query Frame = 2
Query: 866 ARSQCYGHAKSLIKKNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPCA--PETKNNLMATEERCGS---------CGKNRPRIRFRKFCKKHIMIKISIQSKEIHG-EWTRFGVRVLKIW--RIQQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPE---LIVDRKSSALDWSDDWIRRLDMFKMREEQGKC 1831
R QC+GHA + C C H T GP C C +H D PW+ P P + C C CN HA C+F+ ++++ S G+ G C C HHT G HC+ C PG++R+ + PI + C+ C CHP G+ CN +G C CK+GV G CNRC GYQQ++S PC PE L T S R + R++C++ +++ + + E G W R VRVL ++ R Q + + WV AD+ C C LQ G YLL L + + P+ LI+DR AL W W R L + E G C
Sbjct: 155 GRCQCHGHAARCAARARPPRCHCRHHTTGPGCESCRPSHRDWPWRPATPRHP--HPCLPCSCNQHARRCRFNSELFRLSGGRSGGVCERCRHHTAGRHCHYCQPGFWRDPSQPIFSRRACRACQCHPIGAT-GGTCNQTSGQCTCKLGVTGLTCNRCGPGYQQSRSPRMPCQRIPEATTTLATTPGAYSSDPQCQNYCNMSDTRVHMSLRRYCQQDHVLRAQVLASEAAGPAWQRLAVRVLAVYKQRAQPVRRGDQD----------AWVPRADLTCGCLRLQPGTDYLL-----LGSAVGDPDPTRLILDRHGLALPWRPRWARPLKRLQQEERAGGC 475
BLAST of Netrin 1 vs. Ensembl Human
Match: NTN4 (netrin 4 [Source:HGNC Symbol;Acc:HGNC:13658]) HSP 1 Score: 186.037 bits (471), Expect = 2.902e-50 Identity = 160/617 (25.93%), Postives = 247/617 (40.03%), Query Frame = 2
Query: 122 GFSSCFSESGRA-KFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSLLPNSRLIKEWVSVTDIMISLLTDGS-----KKRNKSP--YDHLSISDITIGARSQCYGHAKSLIKKNGTTV-------------CDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGS--LPQ---SYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKS-SSTPCAPETKNNLMATEERCGSCGKNRPRI-------RFRKFCKK-----------------HIMIKISIQSKEIHGEWTRFGVRVLKIWRIQQSLKSQVNLYESEYSIYP-IWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMRE 1816
G S S R K C P N+A +TCGQ + + T + KC KC+ P L+ S + D + + T W S +H I ++L + ++IV F P +MVL + GKTW P+ YF+++C + ++ K + + + +P + P S ++E + +T++ + LL S N+ P + H +I D + C GHA I +G C C H TAG C C +ND+PW+ A N C C CN HA+ C FD +++ S + G C C H+T G +C C PG+YR+ P + P C+ C CHP GS LP ++C+P NG C CK GV G+ C+RC GY PC + + + G C + I FR K H ++KI I S G V++ K+ + +++ ++ + ++YP W + C CP L G YL+ ++ +LIV+ KS W R++ RE
Sbjct: 19 GLSGVAGVSSRCEKACNPRMGNLALGRKLWADTTCGQNATELYCFYSENTDLTCRQPKCDKCNAAYPHLAHLPSAMADSSFRFPRTWWQSAE-----DVHREKIQLDLEAEFYFTHLIVMFKSPRPAAMVLDRSQDFGKTWKPYKYFATNCSAT--------FGLEDDVVKKGAICTSKYSSPFPCTGGEVIFKALSPPYDTEN--PYSAKVQEQLKITNLRVQLLKRQSCPCQRNDLNEEPQHFTHYAIYDFIVKGSCFCNGHADQCIPVHGFRPVKAPGTFHMVHGKCMCKHNTAGSHCQHCAPLYNDRPWEAADGKTGAPNECRTCKCNGHADTCHFDVNVWEASGNRSGGVCDDCQHNTEGQYCQRCKPGFYRDLRRPFSAPDACKPCSCHPVGSAVLPANSVTFCDPSNGDCPCKPGVAGRRCDRCMVGYWGFGDYGCRPC-----DCAGSCDPITGDCISSHTDIDWYHEVPDFRPVHNKSEPAWEWEDAQGFSALLHSVLKIKILSAHDKGTHVEVNVKIKKVLK-----STKLKIFRGKRTLYPESWTDRG---CTCPILNPGLEYLVAGHEDIRT----GKLIVNMKSFVQHWKPSLGRKVMDILKRE 603
BLAST of Netrin 1 vs. Ensembl Human
Match: LAMC1 (laminin subunit gamma 1 [Source:HGNC Symbol;Acc:HGNC:6492]) HSP 1 Score: 190.274 bits (482), Expect = 4.340e-50 Identity = 134/436 (30.73%), Postives = 211/436 (48.39%), Query Frame = 2
Query: 125 FSSCFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLN--EGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIH---SINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSL--LPNSRLIKEWVSVTDIMISLL---TDGSKKRN-----KSPYDHLSISDITIGARSQCYGHAKSLIKKN-GTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQ-TKSSSTPCA 1381
C E GR + C P+F+N A N T T+TCG + +G+ C CD P L + + D N Q T W S + +G+ SIN+T++L K +++ Y+ ++F P+S +YKR + W+P+ Y+S SC+ ++ + + FS + +T + F+ + GR S NS +++EWV+ TDI ++L T G + N KS Y +ISD +G R +C GHA +K VC+C H T G DC +CL ND+PW+ + + + C C CN ++EC FD ++Y+++ HG C +C +T G HC C ++R + + + C C C P GSL + C+ G C CK GV G C+RC+ G+ T++ PC+
Sbjct: 37 MDECTDEGGRPQRCMPEFVNAAFNVTVVATNTCGTPP------EEYCVQTGVTGVTKSCHLCDAGQPHLQHGAAFLTDYNNQADTTWWQSQTM--LAGVQYPSSINLTLHLGKAFDITYVRLKFHTSRPESFAIYKRTREDGPWIPYQYYSGSCENTYSKANRGFIRTGGDEQQALCTDEFS---DISPLTGG--NVAFSTLEGRPSAYNFDNSPVLQEWVTATDIRVTLNRLNTFGDEVFNDPKVLKSYY--YAISDFAVGGRCKCNGHASECMKNEFDKLVCNCKHNTYGVDCEKCLPFFNDRPWRRA--TAESASECLPCDCNGRSQECYFDPELYRSTG--HGGHCTNCQDNTDGAHCERCRENFFR-----LGNNEACSSCHCSPVGSL-STQCD-SYGRCSCKPGVMGDKCDRCQPGFHSLTEAGCRPCS 446 HSP 2 Score: 65.0846 bits (157), Expect = 3.723e-10 Identity = 56/206 (27.18%), Postives = 89/206 (43.20%), Query Frame = 2
Query: 878 CYGHAKSLIKKNGTTVCDCLHKTAGPDCSQC---------LKTHND-QPW---------------KIIFPSEPAGNICERC-FCNNH--------------AEECKFDEKIYKNSSG---KHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQ-SYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKS 1363
C GH+++ + G VC+C TAGP C +C T +D QP +++ + P G +RC C++ C+ + I N+ G + CL C+++T G +C+ C G++ N P N C+ C C+ YG++ Q S CNP G C+C V G+ C C G+ +S
Sbjct: 726 CNGHSETCDPETG--VCNCRDNTAGPHCEKCSDGYYGDSTAGTSSDCQPCPCPGGSSCAVVPKTKEVVCTNCPTGTTGKRCELCDDGYFGDPLGRNGPVRLCRLCQCSDNIDPNAVGNCNRLTGECLKCIYNTAGFYCDRCKDGFFGNPLAP-NPADKCKACNCNLYGTMKQQSSCNPVTGQCECLPHVTGQDCGACDPGFYNLQS 928 HSP 3 Score: 52.7582 bits (125), Expect = 2.197e-6 Identity = 47/150 (31.33%), Postives = 64/150 (42.67%), Query Frame = 2
Query: 917 TTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRC--LHCLHHTTGPHCNSCSPGYYRNRAFPINH----PQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGY 1348
T C+CL G DC C P F + +G CERC C HA +++G+ R C TG HC C +NH P+ C+ C CHP GSL S D+G C+C+ G G C++C++ Y
Sbjct: 902 TGQCECLPHVTGQDCGAC------DPG---FYNLQSGQGCERCDC--HA---------LGSTNGQCDIRTGQCECQPGITGQHCERCE----------VNHFGFGPEGCKPCDCHPEGSL--SLQCKDDGRCECREGFVGNRCDQCEENY 1019
BLAST of Netrin 1 vs. Ensembl Celegans
Match: unc-6 (Netrin unc-6 [Source:UniProtKB/Swiss-Prot;Acc:P34710]) HSP 1 Score: 291.967 bits (746), Expect = 7.062e-90 Identity = 184/591 (31.13%), Postives = 298/591 (50.42%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSLLPNSRLIKEWVSVTDIMI-------------SLLTDGSKKRNKSPYD-----HLSISDITIGARSQCYGHA-KSLIKKNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPCAP-ETKNNLMAT-----EERCGSCGKNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRIQQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKC 1831
C +GR C P+FIN A + TCG D+ C G+ +C CD R S SL+ D N G+ T WVS P S ++++T++L K +E+ Y+ + F +LPDSM LYK GKTW P+ ++SS C++ F + N+ C + + N PF NS ++++WV+ TDI + L D + N++ + S+ ++ +G R +C GHA + + K G CDC H TAG +C C H D+PW + + N C C CN HA+ C+FD ++++ S + G CL+C H+T G +C+ C PG+ R+ + P+ H + C+ CGCHP GSL +S CN +G C CK GV G CNRC KGYQQ++S+ TPC TK + + + +++C C R+ +KFCK+ +++ + S+E+ W ++ + V +++ + N+ E S +W+ + CKCP L++G YLLL K + + L+V+ ++ ++W DD + ++ F +++ G+C
Sbjct: 37 CHDHTGRPVRCVPEFINAAFGKPVIASDTCGTN-RPDKYCTVKEGPDGIIREQCDTCDARNHFQSHPASLLTDLNSIGNMTCWVS--TPSLSP-QNVSLTLSLGKKFELTYVSMHFCSRLPDSMALYKSADFGKTWTPFQFYSSECRRIFGRDP----DVSITKSNEQEAVCTA--SHIMGPGGNRVAFPFLENRPSAQNFENSPVLQDWVTATDIKVVFSRLSPDQAELYGLSNDVNSYGNETDDEVKQRYFYSMGELAVGGRCKCNGHASRCIFDKMGRYTCDCKHNTAGTECEMCKPFHYDRPWGRA--TANSANSCVACNCNQHAKRCRFDAELFRLSGNRSGGVCLNCRHNTAGRNCHLCKPGFVRDTSLPMTHRKACKSCGCHPVGSLGKS-CNQSSGQCVCKPGVTGTTCNRCAKGYQQSRSTVTPCIKIPTKADFIGSSHSEEQDQCSKCRIVPKRLNQKKFCKRDHAVQMVVVSREMVDGWAKYKIVVESVFK-----RGTENMQRGETS---LWISPQGVICKCPKLRVGRRYLLLGKNDSDH--ERDGLMVNPQTVLVEWEDDIMDKVLRFSKKDKLGQC 604
BLAST of Netrin 1 vs. Ensembl Celegans
Match: unc-6 (Netrin unc-6 [Source:UniProtKB/Swiss-Prot;Acc:P34710]) HSP 1 Score: 291.967 bits (746), Expect = 7.062e-90 Identity = 184/591 (31.13%), Postives = 298/591 (50.42%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSLLPNSRLIKEWVSVTDIMI-------------SLLTDGSKKRNKSPYD-----HLSISDITIGARSQCYGHA-KSLIKKNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPCAP-ETKNNLMAT-----EERCGSCGKNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRIQQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKC 1831
C +GR C P+FIN A + TCG D+ C G+ +C CD R S SL+ D N G+ T WVS P S ++++T++L K +E+ Y+ + F +LPDSM LYK GKTW P+ ++SS C++ F + N+ C + + N PF NS ++++WV+ TDI + L D + N++ + S+ ++ +G R +C GHA + + K G CDC H TAG +C C H D+PW + + N C C CN HA+ C+FD ++++ S + G CL+C H+T G +C+ C PG+ R+ + P+ H + C+ CGCHP GSL +S CN +G C CK GV G CNRC KGYQQ++S+ TPC TK + + + +++C C R+ +KFCK+ +++ + S+E+ W ++ + V +++ + N+ E S +W+ + CKCP L++G YLLL K + + L+V+ ++ ++W DD + ++ F +++ G+C
Sbjct: 37 CHDHTGRPVRCVPEFINAAFGKPVIASDTCGTN-RPDKYCTVKEGPDGIIREQCDTCDARNHFQSHPASLLTDLNSIGNMTCWVS--TPSLSP-QNVSLTLSLGKKFELTYVSMHFCSRLPDSMALYKSADFGKTWTPFQFYSSECRRIFGRDP----DVSITKSNEQEAVCTA--SHIMGPGGNRVAFPFLENRPSAQNFENSPVLQDWVTATDIKVVFSRLSPDQAELYGLSNDVNSYGNETDDEVKQRYFYSMGELAVGGRCKCNGHASRCIFDKMGRYTCDCKHNTAGTECEMCKPFHYDRPWGRA--TANSANSCVACNCNQHAKRCRFDAELFRLSGNRSGGVCLNCRHNTAGRNCHLCKPGFVRDTSLPMTHRKACKSCGCHPVGSLGKS-CNQSSGQCVCKPGVTGTTCNRCAKGYQQSRSTVTPCIKIPTKADFIGSSHSEEQDQCSKCRIVPKRLNQKKFCKRDHAVQMVVVSREMVDGWAKYKIVVESVFK-----RGTENMQRGETS---LWISPQGVICKCPKLRVGRRYLLLGKNDSDH--ERDGLMVNPQTVLVEWEDDIMDKVLRFSKKDKLGQC 604
BLAST of Netrin 1 vs. Ensembl Celegans
Match: lam-2 (Laminin-like protein lam-2 [Source:UniProtKB/Swiss-Prot;Acc:Q18823]) HSP 1 Score: 176.792 bits (447), Expect = 4.321e-46 Identity = 129/439 (29.38%), Postives = 208/439 (47.38%), Query Frame = 2
Query: 134 CFSESGRA-KFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGI-HSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSL--LPNSRLIKEWVSVTDIMISL--LTDGSKKRNKSPYD----HLSISDITIGARSQCYGHAK-----SLIKKNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKG-YQQTKSSSTPCAPETKNNL 1402
C+ + R + C PDF+N A N +VT+TCG K T ++ +T C CDDR S + D N+ +ET W S + + + N+T+ L K +++ Y+ ++F P+S +YK+ W PW ++S SC+ ++ + P+ N+ + C + IT + F+ + GR S S ++++WV+ + I ISL + + K P + +ISD +G R +C GHA S + VC C H T G DC++CL +ND+PW+ E N C C C+ + C FD+++++ + HG C+ C +T G HC C ++R C CGC+ GSL + C+ + G CQCK GV G+ C++C G Y + + C ET +L
Sbjct: 40 CYDRATRQPQRCVPDFVNAAFNLEVQVTNTCGTKRPTKFCVQSGHTGQ---RSVCETCDDRHEGFSHPAKYLTDFNVGNNETWWQSDTMQEGQQYPTTTNLTLVLGKSFDITYVRLKFISPRPESFTIYKKTHTDSEWEPWQFYSGSCRATYGLSDRAPI----LPGNEATAQCTKEFSDISPITGG--NIAFSTLEGRPSAHAFEESEVLQKWVTASAIRISLNRMNTFGDEVFKDPQVLRSYYYAISDFAVGGRCKCNGHASECVGSSSVDGENRLVCRCEHNTQGADCNECLPFYNDRPWRSGTSVE--ANECIACNCSQLSNRCYFDQQLFEETG--HGGHCIDCQGNTQGVHCEQCIANHWRRPG-----ENYCVACGCNEIGSLS-TQCD-NEGKCQCKPGVTGRFCDQCLDGFYDFSTNGCKNCGCETSGSL 458 HSP 2 Score: 52.373 bits (124), Expect = 1.129e-6 Identity = 41/135 (30.37%), Postives = 59/135 (43.70%), Query Frame = 2
Query: 932 CLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRC 1336
C+ T G +C C + W EP GN C+ C C ++ + + G CL + G C+ C+ G+Y I CQ+C C P GS + C+ + G CQCK GV G+ C+RC
Sbjct: 859 CIFNTHGFNCENCKPGY----WGDALI-EPKGN-CQSCGCFAAGTRRPNNDYTLLECNQQDGQ--CDCLPNVIGIQCDQCAHGFYN-----ITSGLGCQECNCDPLGSEGNT-CDVNTGQCQCKPGVTGQRCDRC 979 HSP 3 Score: 49.2914 bits (116), Expect = 9.252e-6 Identity = 54/201 (26.87%), Postives = 77/201 (38.31%), Query Frame = 2
Query: 878 CYGHAKSLIKKNGTTVCDCLHKTAGPDCSQCLKTH------------------NDQP------WKIIFPSEPAGNICERC-------FCN----NHAEECKFDEKIYKNSSG---KHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGS-LPQS-----YCNPDNGICQCKIGVEGKLCNRCKKGY 1348
C+ H+ S ++G+ +C+ H TAG C +C + + ND P +I P G RC F N EC NS G K C C+ +T G +C +C PGY+ + I CQ CGC G+ P + CN +G C C V G C++C G+
Sbjct: 738 CHNHSNSCEAESGSCICE--HNTAGDTCERCARGYYGDALQGTEEDCQKCPCPNDGPCILHADGDVICTECPNGYTGRRCDECSDGYFGNPKDGTECVECACSGNTDPNSIGNCDKITGECKKCIFNTHGFNCENCKPGYWGDAL--IEPKGNCQSCGCFAAGTRRPNNDYTLLECNQQDGQCDCLPNVIGIQCDQCAHGF 934
BLAST of Netrin 1 vs. Ensembl Celegans
Match: lam-3 (LAMinin related. See also lmb; Laminin alpha chain [Source:UniProtKB/TrEMBL;Acc:G5ECE3]) HSP 1 Score: 147.517 bits (371), Expect = 1.809e-36 Identity = 122/439 (27.79%), Postives = 182/439 (41.46%), Query Frame = 2
Query: 140 SESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRN-----WYTSSGLNEGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHS----VQNITSNIKVLPFNPMTGRTSL---------LPNSRLIKEWVSVTDIMISLLTDGSKKRNKSPYDHLSISDITIGARSQCYGHAKSLIKK--NGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKN-------SSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDN--------GICQCKIGVEGKLCNRCKKGYQ 1351
SES R F P+ N+A N+ T TCGQ + + C+ ++ +C CD I D G W S + +NITI+L + Y+V YII++ + ++ +G+ + PW Y++ + + P F K + ++S ++N + ++ P TSL RLI D+MI S ++ + SISDI+IG + CYGHA+S G C+C H T G C++C N PWK S P N+C++C C NHA C +D+++ +N + G RCL C H+T G +C C GYYR C+ C C P GS+ + D G C CK G G+ C RC GY+
Sbjct: 42 SESERGLF--PNIFNLATNSLITATDTCGQY-TAEEYCKLVEHVLLRKTTNTQSPQCDICDANNVHKRHPIEYAID----GTRRWWQSPSLANGLRFEKVNITIDLRQEYQVAYIILKMGNSPRPGTWVLEKSLDGEYYEPWQYYAMQDAECMRQFGI-PATTGVPRFQKEDEVHCTSEYSKITPLENGEIHTSLVNGRPGAENTSLELQKFTRARFVRLRLISPRTLNADLMIINKKSDSLDKSVTMRYFYSISDISIGGQCICYGHAESCPSDPVTGQFKCECRHNTCGESCNRCCPLFNQLPWKPGTNSHP--NVCQQCQCFNHAHSCVYDDELDRNKWSITPEGVYEGGGRCLECAHNTEGFNCERCKDGYYRPSGLSHYREDACRTCECDPVGSVSDACVRDDQSAENGQKPGDCICKPGFGGRRCERCAPGYR 470 HSP 2 Score: 75.485 bits (184), Expect = 1.068e-13 Identity = 62/185 (33.51%), Postives = 77/185 (41.62%), Query Frame = 2
Query: 860 IGARSQCYGHAKSLIKKNGTTVC-DCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPCAPETKNNLMAT 1411
IG C H S T+VC DC H T G C CL P I E N C +C C E F + G+ G C C TG +C +C GYY + P + C C CHP GSL CNP +G C+CK GV G+ C+ C++ + T C LM T
Sbjct: 1534 IGWSEPCSCHGHSQTCNPDTSVCTDCEHNTFGDFCEHCL------PGYIGDAREGGANACTKCAC--PLVENSFSDSCVAVDHGR-GYVCNSCKPGYTGQYCETCVAGYYGD---PQHIGGTCSPCDCHPDGSL-HGACNPLSGQCECKPGVTGRTCSMCQERHAFINRVCTSCDQGCYLPLMET 1705 HSP 3 Score: 61.2326 bits (147), Expect = 2.728e-9 Identity = 40/125 (32.00%), Postives = 58/125 (46.40%), Query Frame = 2
Query: 1007 FPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSL-PQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPC 1378
F +P C C CN + D N + G +CL C+ HTTG C SC ++ N C+ CGCH G++ PQ C+ +NG C+CK G C+RCK+ + ++ C
Sbjct: 877 FFEDPLTGKCIECTCNGN-----IDPMGIGNCDSETG-KCLKCIGHTTGDSCESCKEHHWGNAQL-----HTCKPCGCHTQGAVNPQ--CSEENGECECKENYIGAQCDRCKENHGDVENGCPAC 988 HSP 4 Score: 59.3066 bits (142), Expect = 1.047e-8 Identity = 46/162 (28.40%), Postives = 64/162 (39.51%), Query Frame = 2
Query: 914 GTTVCDCLHKTAGPDCSQCLKTH---NDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYC-------------NPDNGIC-QCKIGVEGKLCNRCKKGY 1348
G VC+C AG C C+ + N+Q + G CE+C C+ ++EEC CL+C H+TTG C C PG+ N + C++C C P C N DN +C C +G EG C C G+
Sbjct: 737 GVEVCECPENFAGNSCESCVPGYRRVNNQLY---------GGRCEKCDCHGNSEECD-----------PFTGECLNCRHNTTGSRCELCKPGHVGNPSRG-GELGACEQCECPSLDLNPNPECISTELAVLGSVASNEDNYVCINCPLGYEGNKCEYCSDGF 877 HSP 5 Score: 56.9954 bits (136), Expect = 4.599e-8 Identity = 40/147 (27.21%), Postives = 55/147 (37.41%), Query Frame = 2
Query: 926 CDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRC------LHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGY 1348
C+C G C +C + H D N C C CN+ +G GS C +C G C+ C P Y+ CQ C C+ YGS+ C+ G C+C+ VEG +C +C GY
Sbjct: 960 CECKENYIGAQCDRCKENHGD-----------VENGCPACDCND---------------TGSIGSDCDQVSGQCNCKQGVFGKQCDQCRPSYFNFT------DAGCQFCHCNIYGSIEDGKCDQTTGKCECRENVEGTMCEKCADGY 1074
BLAST of Netrin 1 vs. Ensembl Celegans
Match: lam-1 (LAMinin related. See also lmb [Source:UniProtKB/TrEMBL;Acc:V6CIX7]) HSP 1 Score: 129.798 bits (325), Expect = 9.038e-31 Identity = 106/386 (27.46%), Postives = 158/386 (40.93%), Query Frame = 2
Query: 290 KCLKCDDRIP--------ELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXP--LHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTS-LLPNSRLIKEWVSVTDIMISLLTDGSKKRNKSPYDHLSISDITIGARSQCYGHAKSLIKKNG----TTV----------CDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDN--------GICQCKIGVEGKLCNRCKKGY 1348
KC CD R LS I V + W +G +++I+ +L + ++I+ F P +M++ + GKTW + YF+ C SF P H ++ S S + + ++ NP S LL + L + + + LL + K Y +I +I + CYGHA I + TV C+C+H T G +C +C +ND PW+ E N C +C CN HA C FD +Y++S G C C+H+T G +C C P +YR+ I+ P VC C C GS + C + G C CK V+G C+RCK GY
Sbjct: 77 KCFYCDSRTEWKPQREPYRLSHRIENVVTEVMDDKNRNWYQSE----NGAQNVSISFDLEAEFHFTHLIMTFKSFRPAAMIIERSADFGKTWQVYRYFAYDCDSSFPGIPEGPPKKHTDVICTSQYSDVAPSTGGEIVYKVISPHIVTENPYADEISTLLKITNLRFNFTKLHTLGDDLLDYRPEIDEKYYY---AIYEIVVRGSCSCYGHASRCIPIDPHVSPNTVMERADIVHGRCECMHNTEGLNCEKCKAFYNDLPWRPAIGDEK--NECRQCNCNRHALRCHFDRAVYESSGFVSGGVCDDCMHNTQGKNCEQCKPFFYRDPRRTIDDPHVCLPCECDKAGSQNKGICEGEEDAERGLVAGKCYCKTNVDGNRCDRCKNGY 453 HSP 2 Score: 66.6254 bits (161), Expect = 4.761e-11 Identity = 46/168 (27.38%), Postives = 70/168 (41.67%), Query Frame = 2
Query: 863 GARSQCYGHAKSLIKKNGTT-----VCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGS-LPQSYCNPDNGICQCKIGVEGKLCNRCKKGY 1348
G + Y HA + +N VC+C G C +C + H P ++ G CERC CN + + + S CL CLHHT G C C GYY + + CQ+C C+ G+ + C+ +G C C + G C++C + +
Sbjct: 928 GGPTSGYQHADTCYLRNSGNNTQDIVCNCKSGYQGERCGECAQNHWGSPREV-------GGTCERCDCNGNID------MAMEGSCDAATGECLKCLHHTEGAQCEHCVDGYYGDAKL-----KTCQRCVCNELGTNSTKGACDRVSGQCPCHDNIIGMQCDQCAENH 1077 HSP 3 Score: 50.447 bits (119), Expect = 4.617e-6 Identity = 42/158 (26.58%), Postives = 60/158 (37.97%), Query Frame = 2
Query: 926 CDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRC------LHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPCA 1381
C C G C QC + H + +G CE C C+++ + N G +C C G C+ C Y+ + P C +C C+P GS C+ +NG C+C+ G G LCN C +GY PC
Sbjct: 1059 CPCHDNIIGMQCDQCAENHFNLA---------SGAGCEACGCDSNG--------VVLNHEGVPHLQCNIFDGQCQCKPGRGGRKCDQCEDLYWGDPTTPDG----CHRCECNPTGS-KSLQCHRNNGTCECQAGSGGALCNECARGYTGQWPYCNPCG 1194
BLAST of Netrin 1 vs. Ensembl Fly
Match: NetA (gene:FBgn0015773 transcript:FBtr0073942) HSP 1 Score: 227.639 bits (579), Expect = 9.528e-65 Identity = 189/709 (26.66%), Postives = 293/709 (41.33%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFA--GQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSL--LPNSRLIKEWVSVTDI--------------MISLLTDGSKKRNKSPYD---------------------------HLSISDITIGARSQCYGHA-KSLIKKNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPC--APETKNNLMATE-------------------------------------ERCGSCGKNRPRIRFRKFCKKH--IMIKI-------SIQSKEIHGEWTRFGVRVLKIWRIQQ-SLKSQV-NLYESEYSIYP-------------------------------------------------IW-VENADIRCKCPNLQLGHTYLLLTKIKLK--NYLN-QPELIVDRKSSALDWSDDWIRRLDMFKMR 1813
C++++ + C PDF+N A +A +STCG G+ Y +E C CD P S + D N + T W S P ++ +T++L K +E+ Y+I++ PDSMV+YK +G +W P+ +FSS C++ F P N+ C + + + F+ + GR S L +S ++++WV+ TDI ++SL G+ Y H +ISD ++G R +C GHA K +G C+C H TAG DC +C H D+PW E N C+ C CN HA +C+F+ +I++ S G G C +C H TTG +C+ C G+YR+ P+ H +VC+ C CHP GS + CN +G C CK GV G CNRC +GYQQ++S PC P N++ T+ CG C + R+ KFCK+ IM K+ S+E+ V ++ ++Q S +S + +YE + + YP W + D+ C+CP +++ +YL+L + YL P IV ++W +DW RR+ F+ R
Sbjct: 40 CYNKAHEPRACIPDFVNAAYDAPVVASSTCGSSGA------QRYCEYQDHERSCHTCDMTDPLRSFPARSLTDLNNSNNVTCWRSE--PVTGSGDNVTLTLSLGKKFELTYVILQLCPHAPRPDSMVIYKSTDHGLSWQPFQFFSSQCRRLFGR----PARQSTGRHNEHEARCSDVTRPL------VSRIAFSTLEGRPSSRDLDSSPVLQDWVTATDIRVVFHRLQRPDPQALLSLEAGGATDLASGKYSVPLANGPAGNNIEANLGGDVATSGSGLHYAISDFSVGGRCKCNGHASKCSTDASGQLNCECSHNTAGRDCERCKPFHFDRPWARATAKE--ANECKECNCNKHARQCRFNMEIFRLSQGVSGGVCQNCRHSTTGRNCHQCKEGFYRDATKPLTHRKVCKACDCHPIGSSGK-ICNSTSGQCPCKDGVTGLTCNRCARGYQQSRSHIAPCIKQPPRMINMLDTQNTAPEPDEPESSPGSGGDRNGAAGMAAQSQYYRTEGGRECGKCRVSTKRLNLNKFCKRDYAIMAKVIGRDTSSEAVSREVQRRAMDPDVADYEMDQVQPGSARSPITGVYEFQAADYPNPNPNPRGSEMERFDLQIQAVFKRSRPGESSGAGNVYGMPNTTLKRGPMTWIIPTKDLECRCPRIRVNRSYLILGRDSEAPPGYLGIGPHSIV------IEWKEDWYRRMKRFQRR 721
BLAST of Netrin 1 vs. Ensembl Fly
Match: NetA (gene:FBgn0015773 transcript:FBtr0346088) HSP 1 Score: 227.639 bits (579), Expect = 9.528e-65 Identity = 189/709 (26.66%), Postives = 293/709 (41.33%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFA--GQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSL--LPNSRLIKEWVSVTDI--------------MISLLTDGSKKRNKSPYD---------------------------HLSISDITIGARSQCYGHA-KSLIKKNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPC--APETKNNLMATE-------------------------------------ERCGSCGKNRPRIRFRKFCKKH--IMIKI-------SIQSKEIHGEWTRFGVRVLKIWRIQQ-SLKSQV-NLYESEYSIYP-------------------------------------------------IW-VENADIRCKCPNLQLGHTYLLLTKIKLK--NYLN-QPELIVDRKSSALDWSDDWIRRLDMFKMR 1813
C++++ + C PDF+N A +A +STCG G+ Y +E C CD P S + D N + T W S P ++ +T++L K +E+ Y+I++ PDSMV+YK +G +W P+ +FSS C++ F P N+ C + + + F+ + GR S L +S ++++WV+ TDI ++SL G+ Y H +ISD ++G R +C GHA K +G C+C H TAG DC +C H D+PW E N C+ C CN HA +C+F+ +I++ S G G C +C H TTG +C+ C G+YR+ P+ H +VC+ C CHP GS + CN +G C CK GV G CNRC +GYQQ++S PC P N++ T+ CG C + R+ KFCK+ IM K+ S+E+ V ++ ++Q S +S + +YE + + YP W + D+ C+CP +++ +YL+L + YL P IV ++W +DW RR+ F+ R
Sbjct: 40 CYNKAHEPRACIPDFVNAAYDAPVVASSTCGSSGA------QRYCEYQDHERSCHTCDMTDPLRSFPARSLTDLNNSNNVTCWRSE--PVTGSGDNVTLTLSLGKKFELTYVILQLCPHAPRPDSMVIYKSTDHGLSWQPFQFFSSQCRRLFGR----PARQSTGRHNEHEARCSDVTRPL------VSRIAFSTLEGRPSSRDLDSSPVLQDWVTATDIRVVFHRLQRPDPQALLSLEAGGATDLASGKYSVPLANGPAGNNIEANLGGDVATSGSGLHYAISDFSVGGRCKCNGHASKCSTDASGQLNCECSHNTAGRDCERCKPFHFDRPWARATAKE--ANECKECNCNKHARQCRFNMEIFRLSQGVSGGVCQNCRHSTTGRNCHQCKEGFYRDATKPLTHRKVCKACDCHPIGSSGK-ICNSTSGQCPCKDGVTGLTCNRCARGYQQSRSHIAPCIKQPPRMINMLDTQNTAPEPDEPESSPGSGGDRNGAAGMAAQSQYYRTEGGRECGKCRVSTKRLNLNKFCKRDYAIMAKVIGRDTSSEAVSREVQRRAMDPDVADYEMDQVQPGSARSPITGVYEFQAADYPNPNPNPRGSEMERFDLQIQAVFKRSRPGESSGAGNVYGMPNTTLKRGPMTWIIPTKDLECRCPRIRVNRSYLILGRDSEAPPGYLGIGPHSIV------IEWKEDWYRRMKRFQRR 721
BLAST of Netrin 1 vs. Ensembl Fly
Match: NetA (gene:FBgn0015773 transcript:FBtr0308337) HSP 1 Score: 215.698 bits (548), Expect = 8.626e-61 Identity = 148/475 (31.16%), Postives = 223/475 (46.95%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFA--GQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSL--LPNSRLIKEWVSVTDI--------------MISLLTDGSKKRNKSPYD---------------------------HLSISDITIGARSQCYGHA-KSLIKKNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPC--APETKNNLMATE 1414
C++++ + C PDF+N A +A +STCG G+ Y +E C CD P S + D N + T W S P ++ +T++L K +E+ Y+I++ PDSMV+YK +G +W P+ +FSS C++ F P N+ C + + + F+ + GR S L +S ++++WV+ TDI ++SL G+ Y H +ISD ++G R +C GHA K +G C+C H TAG DC +C H D+PW E N C+ C CN HA +C+F+ +I++ S G G C +C H TTG +C+ C G+YR+ P+ H +VC+ C CHP GS + CN +G C CK GV G CNRC +GYQQ++S PC P N++ T+
Sbjct: 40 CYNKAHEPRACIPDFVNAAYDAPVVASSTCGSSGA------QRYCEYQDHERSCHTCDMTDPLRSFPARSLTDLNNSNNVTCWRS--EPVTGSGDNVTLTLSLGKKFELTYVILQLCPHAPRPDSMVIYKSTDHGLSWQPFQFFSSQCRRLFGR----PARQSTGRHNEHEARCSDVTRPL------VSRIAFSTLEGRPSSRDLDSSPVLQDWVTATDIRVVFHRLQRPDPQALLSLEAGGATDLASGKYSVPLANGPAGNNIEANLGGDVATSGSGLHYAISDFSVGGRCKCNGHASKCSTDASGQLNCECSHNTAGRDCERCKPFHFDRPWARATAKE--ANECKECNCNKHARQCRFNMEIFRLSQGVSGGVCQNCRHSTTGRNCHQCKEGFYRDATKPLTHRKVCKACDCHPIGSSGK-ICNSTSGQCPCKDGVTGLTCNRCARGYQQSRSHIAPCIKQPPRMINMLDTQ 493
BLAST of Netrin 1 vs. Ensembl Fly
Match: LanB2 (gene:FBgn0267348 transcript:FBtr0345031) HSP 1 Score: 172.555 bits (436), Expect = 1.515e-44 Identity = 135/426 (31.69%), Postives = 210/426 (49.30%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHS---INITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSLL--PNSRLIKEWVSVTDIMISL-----LTD---GSKKRNKSPYDHLSISDITIGARSQCYGHAKSLIKKNG-----TTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYY-RNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQ 1354
C+ GR + C P+FIN A E T+TCG++ + + C + N C K +D P S + D + T W S + F GI +N+T++L K Y++ Y+ + F P+S +YKR W+P+ ++S++C+ ++ + S+ + + + + + +S +++ + F+ + GR S + S ++EWV+ TDI I+L D G + KS + +ISDI +GAR +C GHA + G T VC+C H T GPDC +CL +ND WK +E N C+ C CN A++C FD ++ N +G HG CL C + GP+C C +Y R+ + +N C C P GS CN +G CQCK GV G C+RC Y Q
Sbjct: 57 CYDPYGRPQKCLPEFINAAYQLQIESTNTCGEQ-NDNHFCIQTMNQNHKNCEFC-KYNDHNP------SFLTDLHDPQSPTWWQSETM--FEGIQHPNYVNLTLHLGKSYDITYVRILFRSPRPESFTIYKRTSESGPWIPYQFYSATCRDTYSLPDSRAIRKGEGEAHALCTSEYSDISPLRD-----GEIAFSTLEGRPSGINFERSGELQEWVTATDIRITLDRLNTFGDELFGDSQVLKSYF--YAISDIAVGARCKCNGHASKCVPSTGMHGERTLVCECRHNTDGPDCDRCLPLYNDLKWKRSTSTEV--NECKACNCNGLADKCFFDANLF-NRTG-HGGHCLDCRENRDGPNCERCKENFYMRDDGYCVN-------CACDPVGSRSLQ-CNS-HGKCQCKPGVTGDKCDRCDNNYYQ 452 HSP 2 Score: 56.6102 bits (135), Expect = 7.981e-8 Identity = 44/157 (28.03%), Postives = 68/157 (43.31%), Query Frame = 2
Query: 917 TTVC-DCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQ-VCQKCGCHPYGSLPQ----SYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSS 1369
T +C +C G C QC P ++ + C+ C CN + D N + G CL C+H+T G HC+ C G++ + P+ P C +C C+ G+ + C+ G CQCK V G+ C C+ GY +S +
Sbjct: 808 TVICTECPKGYFGSRCEQCSDGFFGDPTGLLGEVQ----TCKSCDCNGN-----VDPNAVGNCNRTTG-ECLKCIHNTAGEHCDQCLSGHFGD---PLALPHGRCDRCSCYEAGTEQDEQSITRCDQVTGQCQCKPNVIGRDCGECQPGYFNIRSGN 951 HSP 3 Score: 50.0618 bits (118), Expect = 8.356e-6 Identity = 50/183 (27.32%), Postives = 60/183 (32.79%), Query Frame = 2
Query: 932 CLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSL--------PQSYCNP--------------------------------------DNGICQCKIGVEGKLCNRCKK 1342
C+H TAG C QCL H P + P G C+RC C E DE+ G C + G C C PGY F I C+ C C P GS Q +C P NG C C VEG+ C+RCK+
Sbjct: 871 CIHNTAGEHCDQCLSGHFGDPLAL-----PHGR-CDRCSCYEAGTE--QDEQSITRCDQVTGQ--CQCKPNVIGRDCGECQPGY-----FNIRSGNGCENCLCDPVGSYNSTCDRYSGQCHCRPGVMGQRCDQCENYFYGFSSEGCKPCECDESGSKGFQCDQNGQCPCNDNVEGRRCDRCKE 1038
BLAST of Netrin 1 vs. Ensembl Fly
Match: LanB2 (gene:FBgn0267348 transcript:FBtr0076382) HSP 1 Score: 172.555 bits (436), Expect = 1.515e-44 Identity = 135/426 (31.69%), Postives = 210/426 (49.30%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHS---INITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSLL--PNSRLIKEWVSVTDIMISL-----LTD---GSKKRNKSPYDHLSISDITIGARSQCYGHAKSLIKKNG-----TTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYY-RNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQ 1354
C+ GR + C P+FIN A E T+TCG++ + + C + N C K +D P S + D + T W S + F GI +N+T++L K Y++ Y+ + F P+S +YKR W+P+ ++S++C+ ++ + S+ + + + + + +S +++ + F+ + GR S + S ++EWV+ TDI I+L D G + KS + +ISDI +GAR +C GHA + G T VC+C H T GPDC +CL +ND WK +E N C+ C CN A++C FD ++ N +G HG CL C + GP+C C +Y R+ + +N C C P GS CN +G CQCK GV G C+RC Y Q
Sbjct: 57 CYDPYGRPQKCLPEFINAAYQLQIESTNTCGEQ-NDNHFCIQTMNQNHKNCEFC-KYNDHNP------SFLTDLHDPQSPTWWQSETM--FEGIQHPNYVNLTLHLGKSYDITYVRILFRSPRPESFTIYKRTSESGPWIPYQFYSATCRDTYSLPDSRAIRKGEGEAHALCTSEYSDISPLRD-----GEIAFSTLEGRPSGINFERSGELQEWVTATDIRITLDRLNTFGDELFGDSQVLKSYF--YAISDIAVGARCKCNGHASKCVPSTGMHGERTLVCECRHNTDGPDCDRCLPLYNDLKWKRSTSTEV--NECKACNCNGLADKCFFDANLF-NRTG-HGGHCLDCRENRDGPNCERCKENFYMRDDGYCVN-------CACDPVGSRSLQ-CNS-HGKCQCKPGVTGDKCDRCDNNYYQ 452 HSP 2 Score: 56.6102 bits (135), Expect = 7.981e-8 Identity = 44/157 (28.03%), Postives = 68/157 (43.31%), Query Frame = 2
Query: 917 TTVC-DCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQ-VCQKCGCHPYGSLPQ----SYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSS 1369
T +C +C G C QC P ++ + C+ C CN + D N + G CL C+H+T G HC+ C G++ + P+ P C +C C+ G+ + C+ G CQCK V G+ C C+ GY +S +
Sbjct: 808 TVICTECPKGYFGSRCEQCSDGFFGDPTGLLGEVQ----TCKSCDCNGN-----VDPNAVGNCNRTTG-ECLKCIHNTAGEHCDQCLSGHFGD---PLALPHGRCDRCSCYEAGTEQDEQSITRCDQVTGQCQCKPNVIGRDCGECQPGYFNIRSGN 951 HSP 3 Score: 50.0618 bits (118), Expect = 8.356e-6 Identity = 50/183 (27.32%), Postives = 60/183 (32.79%), Query Frame = 2
Query: 932 CLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSL--------PQSYCNP--------------------------------------DNGICQCKIGVEGKLCNRCKK 1342
C+H TAG C QCL H P + P G C+RC C E DE+ G C + G C C PGY F I C+ C C P GS Q +C P NG C C VEG+ C+RCK+
Sbjct: 871 CIHNTAGEHCDQCLSGHFGDPLAL-----PHGR-CDRCSCYEAGTE--QDEQSITRCDQVTGQ--CQCKPNVIGRDCGECQPGY-----FNIRSGNGCENCLCDPVGSYNSTCDRYSGQCHCRPGVMGQRCDQCENYFYGFSSEGCKPCECDESGSKGFQCDQNGQCPCNDNVEGRRCDRCKE 1038
BLAST of Netrin 1 vs. Ensembl Zebrafish
Match: ntn1a (netrin 1a [Source:ZFIN;Acc:ZDB-GENE-990415-169]) HSP 1 Score: 312.383 bits (799), Expect = 1.436e-97 Identity = 195/598 (32.61%), Postives = 310/598 (51.84%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGK-CLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIK---------VLPFNPMTGRTSL--LPNSRLIKEWVSVTDIMIS---LLTDGSKKRNKSPYDHLS----ISDITIGARSQCYGHAKSLIK-KNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPC-----APETKNNLMATEE--RCGS---CGKNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRIQQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKCDR 1837
C+ E+G + C PDF+N A +STCG+ S R C T G + C CD P+ + + + D N + T W S ++ ++ +T++L K +EV Y+ ++F P+SM ++K GK+W+P+ Y+S+ C ++ +NK S A + ++ + I ++ ++ F+ + GR S NS ++++WV+ TDI ++ L T G + + S S +SD+ +G R +C GHA +K ++G VC+C H TAGP+C +C H D+PW+ E N C C CN HA C+F+ ++YK S K G CL+C H+T G HC+ C GYYR+ + PI+H + C+ C CHP G+ ++ CN G C CK GV G CNRC GYQQ++S PC AP T +TEE C S K + +I +K+CKK +++ I + GEW +F V ++ +++ +S + + + +WV D+ CKCP ++ G YLLL ++ Q ++ D+ S + W D W RRL F+ RE++GKC +
Sbjct: 40 CYDENGHPRRCIPDFVNAAFGKEVRASSTCGKTPS--RYC--VVTEKGDERHRNCHTCDASDPKKNHPPAYLTDLNNPHNLTCWQSDNYLQYP--QNVTLTLSLGKKFEVTYVSLQFCSPRPESMAIFKSMDYGKSWVPFQYYSTQC---------------RKMYNKPSKATITKQNEQEAICTDSHTDMHPLSGGLIAFSTLDGRPSAHDFDNSPVLQDWVTATDIKVTFSRLHTFGDENEDDSELARDSYFYAVSDLQVGGRCKCNGHASRCVKDRDGNLVCECKHNTAGPECDRCKPFHYDRPWQRATARE--ANECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGYYRDMSKPISHRKACKACDCHPVGAAGKT-CNQTTGQCPCKDGVTGITCNRCANGYQQSRSPIAPCIKIPIAPPT-TTASSTEEPSDCESYCKASKGKLKINMKKYCKKDYAVQVHILKADKAGEWWKFTVNIISVYKQGESRIRRGDQF--------LWVRAKDVACKCPKIKSGKKYLLLGND--EDSPGQSGMVADKGSLVIQWRDTWARRLRKFQQREKKGKCKK 602
BLAST of Netrin 1 vs. Ensembl Zebrafish
Match: ntn1a (netrin 1a [Source:ZFIN;Acc:ZDB-GENE-990415-169]) HSP 1 Score: 312.383 bits (799), Expect = 1.436e-97 Identity = 195/598 (32.61%), Postives = 310/598 (51.84%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGK-CLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIK---------VLPFNPMTGRTSL--LPNSRLIKEWVSVTDIMIS---LLTDGSKKRNKSPYDHLS----ISDITIGARSQCYGHAKSLIK-KNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPC-----APETKNNLMATEE--RCGS---CGKNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRIQQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKCDR 1837
C+ E+G + C PDF+N A +STCG+ S R C T G + C CD P+ + + + D N + T W S ++ ++ +T++L K +EV Y+ ++F P+SM ++K GK+W+P+ Y+S+ C ++ +NK S A + ++ + I ++ ++ F+ + GR S NS ++++WV+ TDI ++ L T G + + S S +SD+ +G R +C GHA +K ++G VC+C H TAGP+C +C H D+PW+ E N C C CN HA C+F+ ++YK S K G CL+C H+T G HC+ C GYYR+ + PI+H + C+ C CHP G+ ++ CN G C CK GV G CNRC GYQQ++S PC AP T +TEE C S K + +I +K+CKK +++ I + GEW +F V ++ +++ +S + + + +WV D+ CKCP ++ G YLLL ++ Q ++ D+ S + W D W RRL F+ RE++GKC +
Sbjct: 40 CYDENGHPRRCIPDFVNAAFGKEVRASSTCGKTPS--RYC--VVTEKGDERHRNCHTCDASDPKKNHPPAYLTDLNNPHNLTCWQSDNYLQYP--QNVTLTLSLGKKFEVTYVSLQFCSPRPESMAIFKSMDYGKSWVPFQYYSTQC---------------RKMYNKPSKATITKQNEQEAICTDSHTDMHPLSGGLIAFSTLDGRPSAHDFDNSPVLQDWVTATDIKVTFSRLHTFGDENEDDSELARDSYFYAVSDLQVGGRCKCNGHASRCVKDRDGNLVCECKHNTAGPECDRCKPFHYDRPWQRATARE--ANECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGYYRDMSKPISHRKACKACDCHPVGAAGKT-CNQTTGQCPCKDGVTGITCNRCANGYQQSRSPIAPCIKIPIAPPT-TTASSTEEPSDCESYCKASKGKLKINMKKYCKKDYAVQVHILKADKAGEWWKFTVNIISVYKQGESRIRRGDQF--------LWVRAKDVACKCPKIKSGKKYLLLGND--EDSPGQSGMVADKGSLVIQWRDTWARRLRKFQQREKKGKCKK 602
BLAST of Netrin 1 vs. Ensembl Zebrafish
Match: ntn1b (netrin 1b [Source:ZFIN;Acc:ZDB-GENE-990415-168]) HSP 1 Score: 307.76 bits (787), Expect = 8.683e-96 Identity = 189/587 (32.20%), Postives = 302/587 (51.45%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGK-CLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSL--LPNSRLIKEWVSVTDIMIS---LLTDGSKKRNKSPYDHLS----ISDITIGARSQCYGHAKSLIK-KNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPC------APETKNNLMATEERCGS---CGKNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRI-QQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKC 1831
C+ E+G + C PDF+N A V+STCG S R C T G + C CD P+ + + + D N + T W S ++ ++ +T++L K +EV Y+ ++F P+SM ++K GKTW+P+ ++S+ CKK + + Q N+ C +Q +T + + F+ + GR S NS ++++WV+ TDI ++ L T G + + S S +SD+ +G R +C GHA +K + G VC+C H TAGP+C +C H D+PW+ E N C C CN HA C+F+ ++YK S + G CL+C H+T G HC+ C GYYR+ + I+H + C+ C CHP G+ ++ CN G C CK GV G CNRC KGYQQ++S PC AP + + C S K + ++ +K+CKK +++ + + GEW +F + ++ +++ ++ L +WV D+ CKCP ++ G YLL+ + ++ Q ++ DR S + W D W RRL F+ R+++GKC
Sbjct: 40 CYDENGNPRRCIPDFVNSAFGKDVRVSSTCGSPPS--RYC--VVTEKGEERSRDCNICDATDPKKTHPPAYLTDLNNPHNLTCWQSENYVQYP--QNVTLTLSLGKKFEVTYVSLQFCSPRPESMAIFKSMDYGKTWVPFQFYSTQCKKMYNKPSKAAITKQ----NEQEAICTDSHTDMQPLTGGL--IAFSTLDGRPSAHDFDNSPVLQDWVTATDIKVTFNRLHTFGDENEDDSELARDSYFYAVSDLQVGGRCKCNGHASKCVKDREGNLVCECKHNTAGPECDRCKPFHYDRPWQRATARE--ANECVACHCNLHARRCRFNMELYKLSGRRSGGVCLNCRHNTAGRHCHYCKEGYYRDMSKAISHRRACKACDCHPVGAAGKT-CNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCIKIPVAAPTSSYSSPDGPTDCDSHCKAPKGKMKVTMKKYCKKDFAVQVHVLKGDKAGEWWKFTINIISVYKQGGHRIRRGDQL---------LWVRAKDVACKCPKIKPGRKYLLMGSDEEEDSRGQSGVVADRGSLLIPWKDLWARRLRKFQQRDKRGKC 602
BLAST of Netrin 1 vs. Ensembl Zebrafish
Match: ntn1b (netrin 1b [Source:ZFIN;Acc:ZDB-GENE-990415-168]) HSP 1 Score: 307.76 bits (787), Expect = 8.683e-96 Identity = 189/587 (32.20%), Postives = 302/587 (51.45%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGK-CLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSL--LPNSRLIKEWVSVTDIMIS---LLTDGSKKRNKSPYDHLS----ISDITIGARSQCYGHAKSLIK-KNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPC------APETKNNLMATEERCGS---CGKNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRI-QQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKC 1831
C+ E+G + C PDF+N A V+STCG S R C T G + C CD P+ + + + D N + T W S ++ ++ +T++L K +EV Y+ ++F P+SM ++K GKTW+P+ ++S+ CKK + + Q N+ C +Q +T + + F+ + GR S NS ++++WV+ TDI ++ L T G + + S S +SD+ +G R +C GHA +K + G VC+C H TAGP+C +C H D+PW+ E N C C CN HA C+F+ ++YK S + G CL+C H+T G HC+ C GYYR+ + I+H + C+ C CHP G+ ++ CN G C CK GV G CNRC KGYQQ++S PC AP + + C S K + ++ +K+CKK +++ + + GEW +F + ++ +++ ++ L +WV D+ CKCP ++ G YLL+ + ++ Q ++ DR S + W D W RRL F+ R+++GKC
Sbjct: 40 CYDENGNPRRCIPDFVNSAFGKDVRVSSTCGSPPS--RYC--VVTEKGEERSRDCNICDATDPKKTHPPAYLTDLNNPHNLTCWQSENYVQYP--QNVTLTLSLGKKFEVTYVSLQFCSPRPESMAIFKSMDYGKTWVPFQFYSTQCKKMYNKPSKAAITKQ----NEQEAICTDSHTDMQPLTGGL--IAFSTLDGRPSAHDFDNSPVLQDWVTATDIKVTFNRLHTFGDENEDDSELARDSYFYAVSDLQVGGRCKCNGHASKCVKDREGNLVCECKHNTAGPECDRCKPFHYDRPWQRATARE--ANECVACHCNLHARRCRFNMELYKLSGRRSGGVCLNCRHNTAGRHCHYCKEGYYRDMSKAISHRRACKACDCHPVGAAGKT-CNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCIKIPVAAPTSSYSSPDGPTDCDSHCKAPKGKMKVTMKKYCKKDFAVQVHVLKGDKAGEWWKFTINIISVYKQGGHRIRRGDQL---------LWVRAKDVACKCPKIKPGRKYLLMGSDEEEDSRGQSGVVADRGSLLIPWKDLWARRLRKFQQRDKRGKC 602
BLAST of Netrin 1 vs. Ensembl Zebrafish
Match: ntn2 (netrin 2 [Source:ZFIN;Acc:ZDB-GENE-050310-2]) HSP 1 Score: 294.664 bits (753), Expect = 7.180e-91 Identity = 182/596 (30.54%), Postives = 297/596 (49.83%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSL--LPNSRLIKEWVSVTDIMI---------SLLTDGSKKRNKS------PYDHLSISDITIGARSQCYGHAKSLIK-KNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPC--APETK-NNLMATEERCGSCG------KNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRIQ-QSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKCDR 1837
C+ E+G A+ C P+FIN A V+S CG+ S R C C CD P + S + D N + T W S + S ++ +T++L K +E+ Y+ ++F P+S+ +YK G+TW P+ ++SS C++ + + Q N+ C + ++ + + F+ + GR S +S ++++WV+VTDI + +L G + + P + ++ D +G R +C GHA ++ K+G +C+C H T GP+C +C H D+PW+ E N C C CN HA C+F+ ++YK S K G C++C H+T G HC+ C G+YR+ I H + C+ C CHP G+ ++ CN G C CK GV G CNRC KGYQQ++S PC P K +++T E C K +I +K+CKK +++S+ E G+W +F V ++ +++ + LK ++ +WV D+ CKCP + +G +L+L + +P L D+ S + W D W RRL F+ +E++GKC +
Sbjct: 26 CYEETGAARRCIPEFINAAFGQEVSVSSVCGRPVS--RSCSLVERDDRPAVRTCQICDASDPRNAHPASYLTDLNSAHNLTCWQSENLQ--SSPLNVTLTLSLGKKFEITYVSLQFCSPRPESLAIYKSMDYGRTWTPYQFYSSQCRRMYNRPNKAAITKQ----NEQEALCTDGHTDLYPLSGGL--IAFSTLDGRPSGKDFDSSPVLQDWVTVTDIRVVFSRPQHTRALPMSGRESEDALGAPGSLPTYYYAVGDFQVGGRCKCNGHASRCVREKDGKLLCECKHNTEGPECDRCKPFHYDRPWQRATGKE--ANECLPCNCNLHARRCRFNMELYKLSGRKSGGVCMNCRHNTAGRHCHYCKEGFYRDMGKAITHRRACKACDCHPVGAAGKT-CNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPVAPCIKIPVIKPTAVISTTEEPADCDSYCKPLKGNLKINMKKYCKKDYAVQVSVLDMETVGDWAKFSVSLMSVYKSRGDPLKRGDHM---------LWVHMKDLACKCPRIHMGKRFLILGSTEGTANPERPGLQADKNSLVIQWRDIWTRRLRKFQRKEKKGKCSK 599
BLAST of Netrin 1 vs. Ensembl Xenopus
Match: rps16 (ribosomal protein S16 [Source:Xenbase;Acc:XB-GENE-5786511]) HSP 1 Score: 299.671 bits (766), Expect = 1.227e-92 Identity = 181/584 (30.99%), Postives = 293/584 (50.17%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSL--LPNSRLIKEWVSVTDIMIS------LLTDGSKKRNKSPYDHLSISDITIGARSQCYGHAKSLIK-KNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPC----APETKNNLMATEERCGSCGKNRP-----RIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRIQQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKC 1831
C+ E G + C P+F+N A N + +STCG+ + CD P S S + D N G+ T W S P+ S ++++T++L K +EV Y+ ++F P+S+ ++K GK+W+P+ Y+SS C+K + + Q N+ C + + +T + + F+ + GR S L NS ++++W++ TDI + L S+ ++ P + S+ + +G R +C GHA K K +CDC H T GP+C +C H D+PW+ E N C C CN HA C+F+ ++YK S K G CL+C H+T G HC+ C G+YR+ + I + C+ C CHP G+ ++ CN G C CK GV G CNRC KGYQQ++S PC A L +TEE +P +I +K+CKK ++++++ E+ G W +F V +L +++ + + + + +W+ D+ CKCP + L YL++ + + +P L D+ S + W D W RRL + RE++GKC
Sbjct: 41 CYDEHGLPRRCVPEFVNAAFNKEVQASSTCGRLATR-------------------HCDTTDPRKSYPASYLTDLNTAGNMTCWRSETFPRHSS-QNVSLTLSLGKKFEVTYVSLQFCSPRPESVAIFKSMDYGKSWVPYQYYSSQCRKVYGKPSRASVTKQ----NEQEALCTDGQTELYPLTGGL--IAFSTLDGRPSAQDLDNSPVLQDWLTATDIRVFFSRPHLLGNKESEASDEGPGFYYSVGEFQVGGRCKCNGHASRCGKDKEARLICDCKHNTEGPECDRCKPFHYDRPWQRATARE--ANECMACTCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGFYRDVSRAITDRRACKACDCHPVGAAGKT-CNQTTGQCPCKDGVTGLTCNRCAKGYQQSRSPIAPCIKIPAVVPTPALTSTEEPADCEQYCKPPKGNYKINLKKYCKKDFVVQVNVLEMEMVGSWAKFTVNILSVYKCRDDRIKRGDNF--------LWIHIKDLSCKCPKILLSRRYLVMGSSEGPS--ERPGLTADKNSMVIQWRDAWTRRLRKLQRREKKGKC 585
BLAST of Netrin 1 vs. Ensembl Xenopus
Match: ntn1 (netrin 1 [Source:NCBI gene;Acc:779839]) HSP 1 Score: 296.59 bits (758), Expect = 2.158e-91 Identity = 184/584 (31.51%), Postives = 293/584 (50.17%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSL--LPNSRLIKEWVSVTDIMISL--LTDGSKKRNKSPYDHLSISDITIGARSQCYGHAKSLIKKNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPCA----PETKNNLMATEERCGSCGK----------NRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRIQQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKC 1831
C+ R K+C PDFINVA ++TCG+ + ++C + S+ + +C CD + PE S S + D + Q T W S F ++ +T+ L + +E+ Y + F PDSM +YK GKTW+P+ ++S+ C++ + + + + C F+ V+ +T + + F P+ GR S S ++++WV+ TDI + L G K + +S+I +G R +C GHA +C+C H T GP+C C + D+PW+ PS + C C CN H+ C+F+ ++YK S K G CL+C H+T G HC+ C GY R+ + PI + C+ C CHP G++ + CN G CQCK GV G CNRC GYQQ++S+ PC T E + G+ G +R + RK+CKK +++ + S E GEW +F VL ++R + +V + + P+WV + D+ C C +Q+G +YL++ + + LI+D+ S AL W D W +L F+ +E +GKC
Sbjct: 42 CYDAKQRPKYCIPDFINVALGQEVITSNTCGR--TPQKLC-YFLDSTNVTSRRCQLCDQKRPENSYPPSYMTDTDAQ---TCWKSESGITFP--QNVTLTLPLGRRFELVYASLRFCSPRPDSMAIYKSMDYGKTWIPFQFYSTHCRRVYDQATTSAISKPMQH----EATCTDFQTGVKPLTKGL--VAFMPLAGRPSARRFEYSPVLQDWVTATDIRVVFNRLHHGKKMGIRRKTAFYGVSEIQVGGRCKCNGHASRCTAGKEGLMCECQHNTIGPECDACKPFYYDRPWQRATPSN--AHECVACECNLHSHRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKQGYKRDLSKPITSRKACKPCQCHPIGAV-NAMCNQTTGQCQCKTGVTGPTCNRCAPGYQQSRSAHMPCVRIQEEMTTTASYPQEWKAGTNGTECQSYCKPSHSRVHMNLRKYCKKDYVLRAQLLSMEKSGEWWQFTATVLTVYR-----QRRVPIRRGD---QPLWVPDHDLACDCLRVQVGKSYLIIGND--EESPDPARLILDKNSLALPWRDVWGHKLRRFQQQERRGKC 598
BLAST of Netrin 1 vs. Ensembl Xenopus
Match: rps16 (ribosomal protein S16 [Source:Xenbase;Acc:XB-GENE-5786511]) HSP 1 Score: 284.648 bits (727), Expect = 3.061e-87 Identity = 175/579 (30.22%), Postives = 283/579 (48.88%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSL--LPNSRLIKEWVSVTDIMIS------LLTDGSKKRNKSPYDHLSISDITIGARSQCYGHAKSLIK-KNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPC----APETKNNLMATEERCGSCGKNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRIQQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKC 1831
C+ E G + C P+F+N A N + +STCG+ + CD P S S + D N G+ T W S P+ S ++++T++L K +EV Y+ ++F P+S+ ++K GK+W+P+ Y+SS C+K + + Q N+ C + + +T + + F+ + GR S L NS ++++W++ TDI + L S+ ++ P + S+ + +G R +C GHA K K +CDC H T GP+C +C H D+PW+ E N C C CN HA C+F+ ++YK S K G CL+C H+T G HC+ C G+YR+ + I + C+ C CHP G+ ++ CN G C CK GV G CNRC KGYQQ++S PC A L +TEE ++++++ E+ G W +F V +L +++ + + + + +W+ D+ CKCP + L YL++ + + +P L D+ S + W D W RRL + RE++GKC
Sbjct: 41 CYDEHGLPRRCVPEFVNAAFNKEVQASSTCGRLATR-------------------HCDTTDPRKSYPASYLTDLNTAGNMTCWRSETFPRHSS-QNVSLTLSLGKKFEVTYVSLQFCSPRPESVAIFKSMDYGKSWVPYQYYSSQCRKVYGKPSRASVTKQ----NEQEALCTDGQTELYPLTGGL--IAFSTLDGRPSAQDLDNSPVLQDWLTATDIRVFFSRPHLLGNKESEASDEGPGFYYSVGEFQVGGRCKCNGHASRCGKDKEARLICDCKHNTEGPECDRCKPFHYDRPWQRATARE--ANECMACTCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGFYRDVSRAITDRRACKACDCHPVGAAGKT-CNQTTGQCPCKDGVTGLTCNRCAKGYQQSRSPIAPCIKIPAVVPTPALTSTEE--------------------PAVVQVNVLEMEMVGSWAKFTVNILSVYKCRDDRIKRGDNF--------LWIHIKDLSCKCPKILLSRRYLVMGSSEGPS--ERPGLTADKNSMVIQWRDAWTRRLRKLQRREKKGKC 560
BLAST of Netrin 1 vs. Ensembl Xenopus
Match: utp4 (UTP4 small subunit processome component [Source:Xenbase;Acc:XB-GENE-977755]) HSP 1 Score: 188.734 bits (478), Expect = 1.144e-49 Identity = 138/430 (32.09%), Postives = 213/430 (49.53%), Query Frame = 2
Query: 143 ESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLN--EGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIH---SINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSL--LPNSRLIKEWVSVTDIMIS---LLTDGSK-----KRNKSPYDHLSISDITIGARSQCYGHAKSLIKKN-GTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQ-TKSSSTPCA 1381
E G + C P+F+N A NAT T+TCG + +G+ C CD L + D N Q T W S + +GI +IN+T++L K +++ Y+ ++F P+S LYKR W+P+ Y+S SC+K+++ + + + FS + +T + F+ + GR S NS +++EWV+ TDI ++ L T G + K KS Y +ISD +G R +C GHA ++ VC+C H T G DC +CL +ND+PW+ P N C C CN ++EC FD ++Y+++ HG C C +T GP+C C YYR ++ + C C C+P GSL + C+ + G C CK GV G+ C+RC+ G+ T++ PCA
Sbjct: 30 EEGSPQRCMPEFVNAAFNATVVATNTCGTPP------EEYCVQTGVTGVTKSCHICDSGQFHLQHGAEYLTDYNNQAEITWWQSQTM--LAGIQYPSTINLTLHLGKAFDITYVRLKFHTSRPESFALYKRTHEDGPWIPYQYYSGSCEKTYQKFNRGFIRTGEDEQQALCTDEFS---DISPLTGG--NVAFSTLEGRPSAYNFDNSPVLQEWVTATDIRVTLNRLNTFGDEVFSDPKVLKSYY--YAISDFAVGGRCKCNGHASECVRNEFEKIVCNCKHNTFGSDCEKCLPFYNDRPWRRSTADSP--NECLPCNCNGRSQECYFDPELYRSTG--HGGHCTGCADNTDGPNCERCRENYYRQ-----DNNEPCHACQCNPVGSL-STQCD-NYGRCSCKPGVMGEKCDRCQPGFHSLTEAGCRPCA 433 HSP 2 Score: 51.9878 bits (123), Expect = 3.282e-6 Identity = 41/143 (28.67%), Postives = 55/143 (38.46%), Query Frame = 2
Query: 923 VCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGIC-QCKIGVEGKLCNRCKKGY 1348
+C C G C +C + + PS+ + C C CN H++ C + + C H+T GPHC CS GYY + CQ C C P GS +C C +G GK C C GY
Sbjct: 676 ICSCPTGYIGQFCERCAPGYRREN-----PSQGPYSPCVLCTCNGHSDTCDPESGV------------CDCQHNTAGPHCERCSEGYYGDST--TGSASDCQPCPC-PGGSSCAVVPRTKEVVCTNCPLGTTGKRCELCDDGY 798
BLAST of Netrin 1 vs. Ensembl Xenopus
Match: utp4 (UTP4 small subunit processome component [Source:Xenbase;Acc:XB-GENE-977755]) HSP 1 Score: 188.734 bits (478), Expect = 1.153e-49 Identity = 138/430 (32.09%), Postives = 213/430 (49.53%), Query Frame = 2
Query: 143 ESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLN--EGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIH---SINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSL--LPNSRLIKEWVSVTDIMIS---LLTDGSK-----KRNKSPYDHLSISDITIGARSQCYGHAKSLIKKN-GTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQ-TKSSSTPCA 1381
E G + C P+F+N A NAT T+TCG + +G+ C CD L + D N Q T W S + +GI +IN+T++L K +++ Y+ ++F P+S LYKR W+P+ Y+S SC+K+++ + + + FS + +T + F+ + GR S NS +++EWV+ TDI ++ L T G + K KS Y +ISD +G R +C GHA ++ VC+C H T G DC +CL +ND+PW+ P N C C CN ++EC FD ++Y+++ HG C C +T GP+C C YYR ++ + C C C+P GSL + C+ + G C CK GV G+ C+RC+ G+ T++ PCA
Sbjct: 39 EEGSPQRCMPEFVNAAFNATVVATNTCGTPP------EEYCVQTGVTGVTKSCHICDSGQFHLQHGAEYLTDYNNQAEITWWQSQTM--LAGIQYPSTINLTLHLGKAFDITYVRLKFHTSRPESFALYKRTHEDGPWIPYQYYSGSCEKTYQKFNRGFIRTGEDEQQALCTDEFS---DISPLTGG--NVAFSTLEGRPSAYNFDNSPVLQEWVTATDIRVTLNRLNTFGDEVFSDPKVLKSYY--YAISDFAVGGRCKCNGHASECVRNEFEKIVCNCKHNTFGSDCEKCLPFYNDRPWRRSTADSP--NECLPCNCNGRSQECYFDPELYRSTG--HGGHCTGCADNTDGPNCERCRENYYRQ-----DNNEPCHACQCNPVGSL-STQCD-NYGRCSCKPGVMGEKCDRCQPGFHSLTEAGCRPCA 442 HSP 2 Score: 63.5438 bits (153), Expect = 1.092e-9 Identity = 52/201 (25.87%), Postives = 85/201 (42.29%), Query Frame = 2
Query: 878 CYGHAKSLIKKNGTTVCDCLHKTAGPDCSQCLKTH-NDQPWKIIFPSEP----AGN-----------ICERCFCNNHAEECKF-DEKIYKNSSGKHGS--------------------------RCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLP-QSYCNPDNGICQCKIGVEGKLCNRCKKGY 1348
C GH+ + ++G VCDC H TAGP C +C + + D +P G+ +C C + C+ D+ + + G++G+ CL C+++T G +C+ C G+Y N N C+ C C+PYG++ QS CN G C+C V + C+ C+ G+
Sbjct: 691 CNGHSDTCDPESG--VCDCQHNTAGPHCERCSEGYYGDSTTGSASDCQPCPCPGGSSCAVVPRTKEVVCTNCPLGTTGKRCELCDDGYFGDPLGENGAPRPCRICECSNNIDPNAVGNCDRLTGECLKCIYNTGGFYCDRCRDGFYGN-PLAQNPDLKCRACSCNPYGTVKGQSGCNQVTGQCECLPHVTERDCSACEPGF 888 HSP 3 Score: 51.9878 bits (123), Expect = 3.573e-6 Identity = 41/143 (28.67%), Postives = 55/143 (38.46%), Query Frame = 2
Query: 923 VCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGIC-QCKIGVEGKLCNRCKKGY 1348
+C C G C +C ++ PS+ + C C CN H++ C + + C H+T GPHC CS GYY + CQ C C P GS +C C +G GK C C GY
Sbjct: 654 ICSCPTGYIGQFCERCAPG-----YRRENPSQGPYSPCVLCTCNGHSDTCDPESGV------------CDCQHNTAGPHCERCSEGYYGDST--TGSASDCQPCPC-PGGSSCAVVPRTKEVVCTNCPLGTTGKRCELCDDGY 776
BLAST of Netrin 1 vs. Ensembl Mouse
Match: Ntn1 (netrin 1 [Source:MGI Symbol;Acc:MGI:105088]) HSP 1 Score: 311.227 bits (796), Expect = 5.265e-97 Identity = 189/590 (32.03%), Postives = 308/590 (52.20%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGK---CLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSL--LPNSRLIKEWVSVTDIMIS---LLTDGSKKRNKSPYD----HLSISDITIGARSQCYGHAKSLIK-KNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPC------APETKNNLMATEERCGS---CGKNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRIQQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKCDR 1837
C E+G + C PDF+N A V+STCG+ + R C S E + C C+ P+ + + + D N + T W S +F H++ +T++L K +EV Y+ ++F P+SM +YK G+TW+P+ ++S+ C+K + P+ Q N+ C ++ ++ + + F+ + GR S NS ++++WV+ TDI ++ L T G + + S + ++SD+ +G R +C GHA ++ ++ + VCDC H TAGP+C +C H D+PW+ E N C C CN HA C+F+ ++YK S K G CL+C H+T G HC+ C G+YR+ PI H + C+ C CHP G+ ++ CN G C CK GV G CNRC KGYQQ++S PC P T + + E C S K + ++ +K+C+K ++I I + G+W +F V ++ +++ S + + S +W+ + DI CKCP ++ YLLL ++ +Q ++ D+ S + W D W RRL F+ RE++GKC +
Sbjct: 41 CSDENGHPRRCIPDFVNAAFGKDVRVSSTCGRPPA--RYC----VVSERGEERLRSCHLCNSSDPKKAHPPAFLTDLNNPHNLTCWQSENYLQFP--HNVTLTLSLGKKFEVTYVSLQFCSPRPESMAIYKSMDYGRTWVPFQFYSTQCRKMYNRPHRAPITKQ----NEQEAVCTDSHTDMRPLSGGL--IAFSTLDGRPSAHDFDNSPVLQDWVTATDIRVAFSRLHTFGDENEDDSELARDSYYYAVSDLQVGGRCKCNGHAARCVRDRDDSLVCDCRHNTAGPECDRCKPFHYDRPWQRATARE--ANECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGFYRDMGKPITHRKACKACDCHPVGAAGKT-CNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCIKIPVAPPTTAASSVEEPEDCDSYCKASKGKLKMNMKKYCRKDYAVQIHILKADKAGDWWKFTVNIISVYKQGTS-----RIRRGDQS---LWIRSRDIACKCPKIKPLKKYLLLGNA--EDSPDQSGIVADKSSLVIQWRDTWARRLRKFQQREKKGKCKK 603
BLAST of Netrin 1 vs. Ensembl Mouse
Match: Ntn1 (netrin 1 [Source:MGI Symbol;Acc:MGI:105088]) HSP 1 Score: 311.227 bits (796), Expect = 5.265e-97 Identity = 189/590 (32.03%), Postives = 308/590 (52.20%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGK---CLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSL--LPNSRLIKEWVSVTDIMIS---LLTDGSKKRNKSPYD----HLSISDITIGARSQCYGHAKSLIK-KNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPC------APETKNNLMATEERCGS---CGKNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRIQQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKCDR 1837
C E+G + C PDF+N A V+STCG+ + R C S E + C C+ P+ + + + D N + T W S +F H++ +T++L K +EV Y+ ++F P+SM +YK G+TW+P+ ++S+ C+K + P+ Q N+ C ++ ++ + + F+ + GR S NS ++++WV+ TDI ++ L T G + + S + ++SD+ +G R +C GHA ++ ++ + VCDC H TAGP+C +C H D+PW+ E N C C CN HA C+F+ ++YK S K G CL+C H+T G HC+ C G+YR+ PI H + C+ C CHP G+ ++ CN G C CK GV G CNRC KGYQQ++S PC P T + + E C S K + ++ +K+C+K ++I I + G+W +F V ++ +++ S + + S +W+ + DI CKCP ++ YLLL ++ +Q ++ D+ S + W D W RRL F+ RE++GKC +
Sbjct: 41 CSDENGHPRRCIPDFVNAAFGKDVRVSSTCGRPPA--RYC----VVSERGEERLRSCHLCNSSDPKKAHPPAFLTDLNNPHNLTCWQSENYLQFP--HNVTLTLSLGKKFEVTYVSLQFCSPRPESMAIYKSMDYGRTWVPFQFYSTQCRKMYNRPHRAPITKQ----NEQEAVCTDSHTDMRPLSGGL--IAFSTLDGRPSAHDFDNSPVLQDWVTATDIRVAFSRLHTFGDENEDDSELARDSYYYAVSDLQVGGRCKCNGHAARCVRDRDDSLVCDCRHNTAGPECDRCKPFHYDRPWQRATARE--ANECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGFYRDMGKPITHRKACKACDCHPVGAAGKT-CNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCIKIPVAPPTTAASSVEEPEDCDSYCKASKGKLKMNMKKYCRKDYAVQIHILKADKAGDWWKFTVNIISVYKQGTS-----RIRRGDQS---LWIRSRDIACKCPKIKPLKKYLLLGNA--EDSPDQSGIVADKSSLVIQWRDTWARRLRKFQQREKKGKCKK 603
BLAST of Netrin 1 vs. Ensembl Mouse
Match: Ntn3 (netrin 3 [Source:MGI Symbol;Acc:MGI:1341188]) HSP 1 Score: 246.899 bits (629), Expect = 5.243e-73 Identity = 166/572 (29.02%), Postives = 262/572 (45.80%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSLLP--NSRLIKEWVSVTDIMISLLTDGSKKRNKS-----PYDHLSISDITIGARSQCYGHA-KSLIKKNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPC------APETKNNLMATEERCGSCGKNRP--RIRFRKFCKKHIMIKISIQSK-EIHGEWTRFGVRVLKIWRIQQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPE----LIVDRKSSALDWSDDWI 1786
C+ E+ + C P +N A +STCG+ S +R+C D P+ + + L+ W S + + ++ +T+ L K +E+ ++ + F P S+ L K +G++W+P +FSSSC + P S CF + Q +L F+ G L NS ++++WV+ TDI I L + + PY + S +++ +G R +C GHA + L+ +G VCDC H T GPDCS+C + D+PW+ E + C C CN HA C+F+ ++Y+ S + G CL+C H+T G HC+ C G+YR+ ++ + C+ C CHP G+ ++ CN G C CK GV G CNRC G+QQ++S PC P +++ + ++ C R RI +KFC+K +++++ ++ E G WTRF V VL ++R + + +WV D C CP L G YLLL + L R S L W D W
Sbjct: 30 CYDEAREPRSCIPGLVNAALGREVLASSTCGR--SANRVC-----------------DSSDPQRAHSADLLTSAPGTASPLCWRSDLLQQ--APFNVTLTVPLGKAFELVFVSLRFCSAPPTSVALLKSQDHGRSWVPLGFFSSSCTLDYGRL---PAPADGPSGPGPEALCFP---APQAQPDGGGLLAFSVQDGSPQGLDLDNSPVLQDWVTATDIRIVLTRPAIQGDTRDGGVTVPYSY-SATELQVGGRCKCNGHASRCLLDTHGHLVCDCQHGTEGPDCSRCKPFYCDRPWQRATGQE--AHACLACSCNGHARRCRFNMELYRLSGRRSGGVCLNCRHNTAGRHCHYCREGFYRDPGRVLSDRRACRACDCHPVGAAGKT-CNQTTGQCPCKDGVTGLTCNRCAPGFQQSRSPVAPCVKTPVPGPTEESSPVEPQDCESHCRPARGSYRISLKKFCRKDYAVQVAVGARGEARGSWTRFPVAVLAVFRSGEE--------RARRGSSALWVPTLDAACGCPRLLPGRRYLLLGGGPGAAAGSTAGRGQGLSAARGSLVLPWRDAWT 562
BLAST of Netrin 1 vs. Ensembl Mouse
Match: Ntn5 (netrin 5 [Source:MGI Symbol;Acc:MGI:2685330]) HSP 1 Score: 191.43 bits (485), Expect = 2.636e-54 Identity = 120/381 (31.50%), Postives = 179/381 (46.98%), Query Frame = 2
Query: 770 VTDIMISLLTDGSKKRNKSPYDHLSISD----ITIGARS--QCYGHAKSLIKKNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPCA--PETKNNLMA------TEERC-GSCGKNRPRIR--FRKFCKKHIMIKISIQ------SKEIHGEWTRFGVRVLKIWRIQQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKL----KNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKC 1831
+TD + G K R + Y + +T G R QC+GHA + C C H T GP C C +H D PW+ P P + C C CN HA C+F+ ++++ S G+ G C C HHT G HC+ C PG++R+ + PI + C+ C CHP G+ CN +G C CK+GV G CNRC GYQQ++S PC PE ++ +C G C + + +++C++ ++ + S+ + EW R V VL +++ Q++ + E+ WV AD+ C C L+ G YLLL + NY + LI++R AL W W R L + +E G C
Sbjct: 1 MTDYRTLFSSPGPKTRIVASYLRVEFGGKAGLVTTGVRGRCQCHGHAARCATRAQPPRCRCRHHTTGPGCESCRPSHRDWPWRPATPQHP--HPCLPCSCNQHARRCRFNSELFRLSGGRSGGVCERCRHHTAGRHCHYCQPGFWRDPSQPITSHKACRACQCHPIGAT-GGMCNQTSGQCSCKLGVTGLTCNRCGPGYQQSRSPRMPCQRIPEATTTPATTPVASRSDPQCQGYCNVSVSSVHMSLQRYCQQDYVLHAQVSASSSQPSEAVGPEWWRLAVHVLAVFK-QRAWPVRRGGQEA-------WVPRADLICGCLRLRPGADYLLLGRAAQTHDDDNY-DPARLILNRHGLALPWRPRWARPLRRLQQKERGGAC 369
BLAST of Netrin 1 vs. Ensembl Mouse
Match: Lamc3 (laminin gamma 3 [Source:MGI Symbol;Acc:MGI:1344394]) HSP 1 Score: 194.512 bits (493), Expect = 9.303e-52 Identity = 146/450 (32.44%), Postives = 215/450 (47.78%), Query Frame = 2
Query: 125 FSSCFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFS-GIH---SINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSL--LPNSRLIKEWVSVTDIMISL--LTDGSKKRNKSPYD----HLSISDITIGARSQCYGHAKSL-IKKNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQ-TKSSSTPCAPETKNNLMATEERCGSC 1432
SC+ GRA+ C P+F N A E + TCG+ D +GL +C +CDD P + S + D + T W S P + G+ S+N+T++L K YE+ Y+ ++F P+S +YKR W P+ Y+S+SC+K++ H + ++ C S + + N + F+ + GR S S +++EWV+ TDI+ISL L K P + ++SD ++G R +C GHA G C C H T G DC +CL D+PW + N C C C+ H+EEC FD ++Y+++ HG C C HTTGPHC C YYR + P CQ C CHP GSL S ++G+C CK V G C+RC G+ ++ PCA +L + R G+C
Sbjct: 31 MGSCYDGVGRAQRCLPEFENAAFGRRAEASHTCGRP-PEDFCPHVGAPGAGL---QCQRCDDADPGRRHDASYLTDFHSPDDSTWWQS---PSMAFGVQYPTSVNLTLSLGKAYEITYVRLKFHTSRPESFAIYKRTYASGPWEPYQYYSASCQKTYGRPEG---HYLRPGEDERVAFCTSEFSDISPL--NGGNVAFSTLEGRPSAYNFEESPVLQEWVTSTDILISLDRLNTFGDDIFKDPRVLQSYYYAVSDFSVGGRCKCNGHASECEPNAAGQLACRCQHNTTGVDCERCLPFFQDRPWARGTAED--ANECLPCNCSGHSEECTFDRELYRSTG--HGGHCQRCRDHTTGPHCERCEKNYYR---WSPKTP--CQPCDCHPAGSL--SLQCDNSGVCPCKPTVTGWKCDRCLPGFHSLSEGGCRPCACNVAGSLGTCDPRSGNC 457 HSP 2 Score: 65.855 bits (159), Expect = 1.620e-10 Identity = 49/159 (30.82%), Postives = 65/159 (40.88%), Query Frame = 2
Query: 890 AKSLIKKNGTTVCD-CLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKS 1363
A + I ++G VC C G C C P + +P C RC C+ + D N H CL CL++TTG HC C G+Y A C C C GS+ + CNP G C C V G+ C+RC G+ +S
Sbjct: 774 ACATIPESGDVVCTHCPPGQRGRRCESCEDGFFGDPLGLSGAPQP----CRRCQCSGN-----VDLNAVGNCD-PHSGHCLRCLYNTTGAHCEHCREGFY-GSAVATRPVDKCAPCSCDLRGSVSEKTCNPVTGQCVCLPYVSGRDCSRCSPGFYDLQS 921 HSP 3 Score: 65.4698 bits (158), Expect = 1.988e-10 Identity = 51/168 (30.36%), Postives = 76/168 (45.24%), Query Frame = 2
Query: 869 RSQCYGHAKSLIKKNGTTVCD--------CLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGY 1348
R QC G+ + N CD CL+ T G C C + + + P + C C C+ EK +G+ C+ CL + +G C+ CSPG+Y + + CQ C CHP GSL ++ C+P G C C+ GV G+ C+RC+ G+
Sbjct: 821 RCQCSGN----VDLNAVGNCDPHSGHCLRCLYNTTGAHCEHCREGF----YGSAVATRPV-DKCAPCSCDLRG---SVSEKTCNPVTGQ----CV-CLPYVSGRDCSRCSPGFYD-----LQSGRGCQSCKCHPLGSL-ENKCHPKTGQCPCRPGVTGQACDRCQLGF 965
BLAST of Netrin 1 vs. UniProt/SwissProt
Match: sp|O95631|NET1_HUMAN (Netrin-1 OS=Homo sapiens OX=9606 GN=NTN1 PE=1 SV=2) HSP 1 Score: 312.768 bits (800), Expect = 8.364e-97 Identity = 193/590 (32.71%), Postives = 307/590 (52.03%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGK---CLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSL--LPNSRLIKEWVSVTDIMIS---LLTDGSKKRNKSPYDHLS----ISDITIGARSQCYGHAKSLIK-KNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPC------APETKNNLMATEERCGS---CGKNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRIQQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKCDR 1837
C E+G + C PDF+N A V+STCG+ + R C S E + C C+ P+ + + + D N + T W S +F H++ +T++L K +EV Y+ ++F P+SM +YK G+TW+P+ ++S+ C+K + P+ Q N+ C ++ ++ + + F+ + GR S NS ++++WV+ TDI ++ L T G + + S S +SD+ +G R +C GHA ++ ++ + VCDC H TAGP+C +C H D+PW+ E N C C CN HA C+F+ ++YK S K G CL+C H+T G HC+ C GYYR+ PI H + C+ C CHP G+ ++ CN G C CK GV G CNRC KGYQQ++S PC P T + + E C S K + +I +K+CKK ++I I + G+W +F V ++ +++ S + + S +W+ + DI CKCP ++ YLLL ++ +Q ++ D+ S + W D W RRL F+ RE++GKC +
Sbjct: 41 CSDENGHPRRCIPDFVNAAFGKDVRVSSTCGRPPA--RYC----VVSERGEERLRSCHLCNASDPKKAHPPAFLTDLNNPHNLTCWQSENYLQFP--HNVTLTLSLGKKFEVTYVSLQFCSPRPESMAIYKSMDYGRTWVPFQFYSTQCRKMYNRPHRAPITKQ----NEQEAVCTDSHTDMRPLSGGL--IAFSTLDGRPSAHDFDNSPVLQDWVTATDIRVAFSRLHTFGDENEDDSELARDSYFYAVSDLQVGGRCKCNGHAARCVRDRDDSLVCDCRHNTAGPECDRCKPFHYDRPWQRATARE--ANECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGYYRDMGKPITHRKACKACDCHPVGAAGKT-CNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCIKIPVAPPTTAASSVEEPEDCDSYCKASKGKLKINMKKYCKKDYAVQIHILKADKAGDWWKFTVNIISVYKQGTS-----RIRRGDQS---LWIRSRDIACKCPKIKPLKKYLLLGNA--EDSPDQSGIVADKSSLVIQWRDTWARRLRKFQQREKKGKCKK 603
BLAST of Netrin 1 vs. UniProt/SwissProt
Match: sp|O09118|NET1_MOUSE (Netrin-1 OS=Mus musculus OX=10090 GN=Ntn1 PE=1 SV=3) HSP 1 Score: 311.227 bits (796), Expect = 3.683e-96 Identity = 189/590 (32.03%), Postives = 308/590 (52.20%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGK---CLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSL--LPNSRLIKEWVSVTDIMIS---LLTDGSKKRNKSPYD----HLSISDITIGARSQCYGHAKSLIK-KNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPC------APETKNNLMATEERCGS---CGKNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRIQQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKCDR 1837
C E+G + C PDF+N A V+STCG+ + R C S E + C C+ P+ + + + D N + T W S +F H++ +T++L K +EV Y+ ++F P+SM +YK G+TW+P+ ++S+ C+K + P+ Q N+ C ++ ++ + + F+ + GR S NS ++++WV+ TDI ++ L T G + + S + ++SD+ +G R +C GHA ++ ++ + VCDC H TAGP+C +C H D+PW+ E N C C CN HA C+F+ ++YK S K G CL+C H+T G HC+ C G+YR+ PI H + C+ C CHP G+ ++ CN G C CK GV G CNRC KGYQQ++S PC P T + + E C S K + ++ +K+C+K ++I I + G+W +F V ++ +++ S + + S +W+ + DI CKCP ++ YLLL ++ +Q ++ D+ S + W D W RRL F+ RE++GKC +
Sbjct: 41 CSDENGHPRRCIPDFVNAAFGKDVRVSSTCGRPPA--RYC----VVSERGEERLRSCHLCNSSDPKKAHPPAFLTDLNNPHNLTCWQSENYLQFP--HNVTLTLSLGKKFEVTYVSLQFCSPRPESMAIYKSMDYGRTWVPFQFYSTQCRKMYNRPHRAPITKQ----NEQEAVCTDSHTDMRPLSGGL--IAFSTLDGRPSAHDFDNSPVLQDWVTATDIRVAFSRLHTFGDENEDDSELARDSYYYAVSDLQVGGRCKCNGHAARCVRDRDDSLVCDCRHNTAGPECDRCKPFHYDRPWQRATARE--ANECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGFYRDMGKPITHRKACKACDCHPVGAAGKT-CNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCIKIPVAPPTTAASSVEEPEDCDSYCKASKGKLKMNMKKYCRKDYAVQIHILKADKAGDWWKFTVNIISVYKQGTS-----RIRRGDQS---LWIRSRDIACKCPKIKPLKKYLLLGNA--EDSPDQSGIVADKSSLVIQWRDTWARRLRKFQQREKKGKCKK 603
BLAST of Netrin 1 vs. UniProt/SwissProt
Match: sp|Q90922|NET1_CHICK (Netrin-1 OS=Gallus gallus OX=9031 GN=NTN1 PE=1 SV=1) HSP 1 Score: 307.375 bits (786), Expect = 1.183e-94 Identity = 192/586 (32.76%), Postives = 305/586 (52.05%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGK-CLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSL--LPNSRLIKEWVSVTDIMIS---LLTDGSKKRNKSPYDHLS----ISDITIGARSQCYGHAKSLIK-KNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPC---APETKNNLMATEERCGSC------GKNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRIQQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKC 1831
C+ E G + C PDF+N A +V+STCG+ S R C T G + + C C+ P+ + S + D N + T W S ++ H++ +T++L K +EV Y+ ++F P+SM +YK GKTW+P+ ++S+ C+K + + Q N+ C V+ ++ + + F+ + GR + NS ++++WV+ TDI ++ L T G + + S S +SD+ +G R +C GHA ++ ++ VCDC H TAGP+C +C H D+PW+ E N C C CN HA C+F+ ++YK S K G CL+C H+T G HC+ C G+YR+ + PI+H + C++C CHP G+ Q+ CN G C CK GV G CNRC KGYQQ++S PC ++ E C K + +I +K+CKK ++I I E + +W +F V ++ +++ + L + + +WV DI CKCP ++ YLLL ++ +Q +I D+ S + W D W RRL F+ RE++GKC
Sbjct: 43 CYDEHGLPRRCIPDFVNSAFGKEVKVSSTCGKPPS--RYC--VVTEKGEEQVRSCHLCNASDPKRAHPPSFLTDLNNPHNLTCWQSDSYVQYP--HNVTLTLSLGKKFEVTYVSLQFCSPRPESMAIYKSMDYGKTWVPFQFYSTQCRKMYNKPSRAAITKQ----NEQEAICTDSHTDVRPLSGGL--IAFSTLDGRPTAHDFDNSPVLQDWVTATDIKVTFSRLHTFGDENEDDSELARDSYFYAVSDLQVGGRCKCNGHASRCVRDRDDNLVCDCKHNTAGPECDRCKPFHYDRPWQRATARE--ANECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGFYRDLSKPISHRKACKECDCHPVGAAGQT-CNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCIKIPAAPPPTAASSTEEPADCDSYCKASKGKLKINMKKYCKKDYAVQIHILKAEKNADWWKFTVNIISVYK-----QGSNRLRRGDQT---LWVHAKDIACKCPKVKPMKKYLLLGST--EDSPDQSGIIADKSSLVIQWRDTWARRLRKFQQREKKGKC 603
BLAST of Netrin 1 vs. UniProt/SwissProt
Match: sp|Q924Z9|NET1_RAT (Netrin-1 OS=Rattus norvegicus OX=10116 GN=Ntn1 PE=1 SV=1) HSP 1 Score: 305.449 bits (781), Expect = 5.722e-94 Identity = 187/590 (31.69%), Postives = 305/590 (51.69%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGK---CLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSL--LPNSRLIKEWVSVTDIMIS---LLTDGSKKRNKSPYD----HLSISDITIGARSQCYGHAKSLIK-KNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPC------APETKNNLMATEERCGS---CGKNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRIQQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKCDR 1837
C E+G + C PDF+N A V+STCG+ + R C S E + C C+ P+ + + + D N + T W S +F H++ +T++L K +EV Y+ ++F P+SM +YK G+TW+P+ ++S+ C+K + P+ Q N+ C ++ ++ + + F+ + GR S NS ++++WV+ TDI ++ L T G + + S + ++SD+ +G R +C GHA ++ ++ + VCDC H TAGP+C +C H D+PW+ E N C C CN HA C+F+ ++YK S K G CL+C H+T G HC+ C G+YR+ PI H + C+ C CHP G+ ++ CN G C CK GV G CNRC KGYQQ++S PC P T + M E C S K + ++ +K+C+K ++I I + G+W +F V ++ +++ S + + S +W+ + DI KCP ++ YLLL ++ +Q ++ D+ + W D W RR F+ RE++GKC +
Sbjct: 41 CSDENGHPRRCIPDFVNAAFGKDVRVSSTCGRPPA--RYC----VVSERGEERLRSCHLCNSSDPKKAHPPAFLTDLNNPHNLTCWQSENYLQFP--HNVTLTLSLGKKFEVTYVSLQFCSPRPESMAIYKSMDYGRTWVPFQFYSTQCRKMYNRPHRAPITKQ----NEQEAVCTDSHTDMRPLSGGL--IAFSTLDGRPSAHDFDNSPVLQDWVTATDIRVAFSRLHTFGDENEDDSELARDSYYYAVSDLQVGGRCKCNGHAARCVRDRDDSLVCDCRHNTAGPECDRCKPFHYDRPWQRATARE--ANECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGFYRDMGKPITHRKACKACDCHPVGAAGKT-CNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCIKIPVAPPTTAASSMEEPEDCDSYCKASKGKLKMNMKKYCRKDYAVQIHILKADKAGDWWKFTVNIISVYKQGTS-----RIRRGDQS---LWIRSRDIAXKCPKIKPLKKYLLLGNA--EDSPDQSGIVADKSXLVIQWRDTWARRXRKFQQREKKGKCKK 603
BLAST of Netrin 1 vs. UniProt/SwissProt
Match: sp|Q2HXW4|NET1_PIG (Netrin-1 OS=Sus scrofa OX=9823 GN=NTN1 PE=2 SV=1) HSP 1 Score: 304.294 bits (778), Expect = 1.336e-93 Identity = 188/585 (32.14%), Postives = 302/585 (51.62%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGK---CLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSL--LPNSRLIKEWVSVTDIMIS---LLTDGSKKRNKSPYDHLS----ISDITIGARSQCYGHAKSLIK-KNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPC------APETKNNLMATEERCGS---CGKNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRIQQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQ 1822
C E+G + C PDF+N A V+STCG+ + R C S E + C C+ P+ + + + D N + T W S +F H++ +T++L K +EV Y+ ++F P+SM +YK G+TW+P+ ++S+ C+K + P+ Q N+ C ++ ++ + + F+ + GR S NS ++++WV+ TDI ++ L T G + + S S +SD+ +G R +C GHA ++ ++ + VCDC H TAGP+C +C H D+PW+ E N C C CN HA C+F+ ++YK S K G CL+C H+T G HC+ C GY+R+ PI H + C+ C CHP G+ ++ CN G C CK GV G CNRC KGYQQ++S PC P T + + E C S K + +I +K+CKK ++I I + G+W +F V ++ +++ S + + +W+ + DI CKCP ++ YLLL ++ +Q ++ D+ S + W D W RRL F+ RE++
Sbjct: 41 CSDENGHPRRCIPDFVNAAFGKDVRVSSTCGRPPA--RYC----VVSERGEERLRSCHLCNASDPKKAHPPAFLTDLNNPHNLTCWQSENYLQFP--HNVTLTLSLGKKFEVTYVSLQFCSPRPESMAIYKSMDYGRTWVPFQFYSTQCRKMYNRPHRAPITKQ----NEQEAVCTDSHTDMRPLSGGL--IAFSTLDGRPSAHDFDNSPVLQDWVTATDIRVAFSRLHTFGDENEDDSELARDSYFYAVSDLQVGGRCKCNGHAARCVRDRDDSLVCDCRHNTAGPECDRCKPFHYDRPWQRATARE--ANECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGYFRDLGKPITHRKACKACDCHPVGAAGKT-CNQTTGQCPCKDGVTGVTCNRCAKGYQQSRSPIAPCIKIPVAPPTTAASSVEEPEDCDSYCKASKGKLKINMKKYCKKDYAVQIHILKADKAGDWWKFTVNIISVYKQGASRIRRGDQN--------LWIRSRDIACKCPKIKPLKKYLLLGNA--EDSPDQSGIVADKSSLVIQWRDTWARRLRKFQQREKK 598
BLAST of Netrin 1 vs. TrEMBL
Match: A0A4D6FZR0 (Netrin-5 (Fragment) OS=Schmidtea mediterranea OX=79327 GN=netrin-5 PE=2 SV=1) HSP 1 Score: 565.844 bits (1457), Expect = 0.000e+0 Identity = 288/288 (100.00%), Postives = 288/288 (100.00%), Query Frame = 2
Query: 152 RAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSLLPNSRLIKEWVSVTDIMISLLTDGSKKRNKSPYDHLSISDITIGARSQCYGHAKSLIKKNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPS 1015
RAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFSSSCKKSFKMKRSKPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSLLPNSRLIKEWVSVTDIMISLLTDGSKKRNKSPYDHLSISDITIGARSQCYGHAKSLIKKNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPS
Sbjct: 1 RAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFSSSCKKSFKMKRSKPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSLLPNSRLIKEWVSVTDIMISLLTDGSKKRNKSPYDHLSISDITIGARSQCYGHAKSLIKKNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPS 288
BLAST of Netrin 1 vs. TrEMBL
Match: Q49BF9 (Netrin 1 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 338.191 bits (866), Expect = 2.444e-103 Identity = 222/652 (34.05%), Postives = 341/652 (52.30%), Query Frame = 2
Query: 128 SSCFSESGRAKFCEPDFINVAQNAT-FEVTSTCGQKGSTDRICRNWYTSSGLNEGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQL-PDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPA-------CFSFKHS-VQNITSNIKVLPFNPMTGRTSLLPN-SRLIKEWVSVTDIMISLL----------TDG-------------------------------SKKRNKSPY----DHLSISDITIGARSQCYGHAKSLIKK--NGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPCAPETKNNLM----ATEERCGSCGKNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRIQQS---------LKSQVNLYESEY--SIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKCDRYQYKRKHSK 1864
S C+SE G+A C P F N+ +N E+TSTCGQ + W +N C+K + +I+ + D+++ + T W S + ++NIT + KL+E+YYI + L PDS+++ K NG +W W+YFS C +SF ++ P+ S +KI+ + C S S + N+ ++ VL F T + + S W+S T+I I+L DG KKRN D +++DI+IG R QCYGHA +K + T CDC H TAG DC C + D+ W + + A C+RC CN H+++C+F+EK+Y S+ + G C+ C H+T G +C+ C+ GY+R+ + P++H VC KC CHP GS+ C+ N C CK GV G CNRC+KG+QQTKS TPC + + A E RC C R RIRF+K+C+K ++ +++QS+E HGE RF ++V ++RI S L S + +++ S +P+W++ D++CKCP+LQLG TYL++ K+ YL + EL++D+KS AL W W RR+ F +E QG C++++ KR++ +
Sbjct: 33 SLCYSE-GKAFRCVPPFTNIIENMIPDEITSTCGQNK------KQWICVDHINCHSCMK-------DTHSINYLTDQHLNNNITFWASDFVNHGD---NVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQ---PMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFT--TNNPNFFSHASDQYNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAE--CKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVDCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMAQAPENRCQHCNNKRKRIRFKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCEKWKLKRRYYR 660
BLAST of Netrin 1 vs. TrEMBL
Match: A0A4S2M7W2 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_002075 PE=4 SV=1) HSP 1 Score: 334.724 bits (857), Expect = 1.379e-100 Identity = 212/667 (31.78%), Postives = 329/667 (49.33%), Query Frame = 2
Query: 128 SSCFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGKCLKC--DDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQN--ITSNIKVLPFNPMTGRTSLLPNSRLIKEWVSVTDIMISL-----LTD------------------------GSKKRNKSPYD----------------------HLSISDITIGARSQCYGHAKSLIKKN------------GTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPC------------------APETKNNLMATEE--RCGSCGKNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRIQQSLKSQVNLYESEYSI--YPIWVE---------NADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKCD 1834
S C SE GRA C P F N+A+ A TSTCG T++ C+ ++G + +C +C +D+ P + DR+ +ET WVSG + + +INIT+++ K +EVYY+ ++ G+LPD++ LYK GKTW PW YFS+ C ++F++ S + Q + N C + K S N ++KV+ F+ GR S P S + +W+++TD+ +SL L D G ++NK H + SD+ IG R +C GH+ ++ G CDC H TAG DC +C + D+PW + N C+ C C NH EC F K +K S G+ G C+ C H+T G HC+ CS G+ RN A P+ H Q C++C CHP GS+ C+ +G C CK GV G+ CNRC++GY+QT+ S PC A + + + TE +C C +R RIRF+KFC++ + ++S G+ TRF +R+ + WR+ + ++ ++ +S+ +P+W++ N IRC CP L+ G +YL T+ + N+ EL++D S + W + W RRL+ F R + +C+
Sbjct: 53 SPCHSE-GRASQCMPPFTNIAEGAPVVATSTCGASDKTEKFCKQQVDATGRIQWRCEQCTSEDKFP-----THHLTDRHQMVNETYWVSGAVLRG---QTINITLSMGKTFEVYYVSLQPYGRLPDAIALYKSSDFGKTWHPWQYFSTDCFRAFRLPTSNEHNAQISAANIQEVLCVALKSSEDNEPYGRSLKVIAFSTTIGRPSSQPWSAALVDWMTMTDLRVSLTRFSDLADMTQHDASGLLLTPNLLSPAEVFRVGEVEKNKMSAGTLGMSRHHFQSDAASFMPDKGIHFAFSDLAIGGRCKCNGHSNRCVRDRLVDAAGKETGEWGPLRCDCQHNTAGIDCEKCAPGYLDRPWARA--TRELANECKPCRCGNHTSECVFSVKHFKQSGGQTGGICVACRHNTEGIHCDQCSAGFTRNPAVPMGHEQACRECRCHPIGSVANEPCDRKSGQCPCKPGVIGQACNRCEEGYKQTRLSDHPCVRVMEHFAYRRKTVPPPVAASSHHQPINTEPHLKCPPCKPHRRRIRFKKFCRREAVFMGKVESYRAEGDKTRFEIRIRETWRVDGA-AGRLLPKKASHSLPPFPVWIQSQLIGGDNGNPAIRCLCPELKTGESYLFFTQPYQIPHGNRMELMLDPSSIIIPWRESWRRRLNKFVRRAAKNRCN 707
BLAST of Netrin 1 vs. TrEMBL
Match: A0A5N5Q759 (Uncharacterized protein OS=Pangasianodon hypophthalmus OX=310915 GN=PHYPO_G00011220 PE=4 SV=1) HSP 1 Score: 326.635 bits (836), Expect = 5.718e-100 Identity = 187/583 (32.08%), Postives = 291/583 (49.91%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSL--LPNSRLIKEWVSVTDIMISLLTDGSKKRNKSPYDHLSISDITIGARSQCYGHAKSLIK-KNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPCAPETKNN---LMATEERCGSC------GKNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRIQQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKCDRYQY 1846
C+SESG A++C P+F+N A +STCG G + Y++ L T W S + ++ +T++L K +E+ YI + F + PDSM +YK G++W P+ ++SS C++ + P + N+ P C +S N + +L F+ + GR S +S ++++W++VTDI ++ K + P ++SD +G R +C GHA I+ K G VCDC H T GP+C +C H D+PW+ P E N C C CN HA C+F+ ++YK S K G C++C H+T G HC+ C GYYR+ + PI H + C C CHP G+ ++ CN +G CQCK GV G CNRC KGYQQ++S PC N L+++ E C K +I +K+CKK + +S+ E G+W +F V ++ +++ ++ LK N +WV+ D+ CKCP + +G +L+L + L D+ S + W D W RRL F+ RE++GKC ++
Sbjct: 41 CYSESGAARYCIPEFVNAAFGQEVVASSTCGHHGCESTHLTDLYSAQNL-------------------------------TCWQSEMLRSMP--FNVTLTLSLGKKFEITYISLHFCSEKPDSMAIYKSMDYGRSWTPFQFYSSQCRRVYDR----PNRAEITKLNEQEPLCMELPNSADNSNA---ILVFSTLDGRPSRTNFVSSPVLQDWITVTDIRVTFARPKMSKDVREPAYFYAVSDFQVGGRCKCNGHAHRCIRDKEGKMVCDCKHNTEGPECDRCKPFHYDRPWQRATPKE--ANECLPCNCNLHARRCRFNMELYKLSGRKSGGVCMNCRHNTAGRHCHYCKEGYYRDMSRPITHRKACTACDCHPVGAAGKT-CNQTSGQCQCKDGVTGLTCNRCAKGYQQSRSPVAPCIKIPTVNPTALVSSTEGPADCESYCRPAKGNLKINTKKYCKKDYAVLVSVLDMETVGDWAKFSVSLVSVYKSREPLKRGENT---------LWVQMKDLACKCPRIHMGKRFLILGSGGGGASAERAGLTADKNSLVIQWRDIWTRRLRKFQRREKKGKCSAAEH 571
BLAST of Netrin 1 vs. TrEMBL
Match: W5UHM1 (Netrin-1 OS=Ictalurus punctatus OX=7998 GN=NTN1 PE=2 SV=1) HSP 1 Score: 325.865 bits (834), Expect = 2.124e-99 Identity = 193/585 (32.99%), Postives = 299/585 (51.11%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSL--LPNSRLIKEWVSVTDIMISLLTDGSKKRNKSPYDH-----LSISDITIGARSQCYGHAKSLIK-KNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPC------APETKNNLMATEERCGS---CGKNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRIQQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKCDR 1837
C+ E+G+A+ C PDF+N A +STCGQK + R C G +C CD P+ S + D + + T W S ++ ++ +T++L K +EV Y+ +F PDSM ++K GKTW+P+ Y+SS C+K + + Q + A + Q S ++ F+ + GR S NS ++++WV+ TDI I+ ++R D ++SD+ +G R +C GHA ++ + G VC+C H TAGP+C +C H D+PW+ E N C C CN HA C+F+ ++YK S K G CL+C H+T G HC+ C GYYR+ + PI H + C+ C CHP G+ ++ CN G C CK GV G CNRC KGYQQ++S PC P T ++ C S K + +I +K+CKK +++ I + GEW +F V ++ +++ +S + + +WV D+ CKCP ++ G YLLL ++ Q ++ D+ S + W D W RRL F+ RE++GKC +
Sbjct: 36 CYDENGQARRCIPDFVNAAFGREVRASSTCGQK-TPSRYCLGTERGGGERSRECRMCDASDPKRRHPASHLTDLHNPHNLTCWQSENGIRYP--QNVTLTLSLGKKFEVTYVSAQFCSIRPDSMAIFKSMDYGKTWVPFQYYSSQCRKVYNKPNRAAITKQNEQEAVCTDAHQQQQPHHQQPVSG-GLVAFSTLDGRPSAHDFDNSPVLQDWVTATDIRITFSRLHGEERKAEAEDEDDSHFYAVSDLQVGGRCKCNGHASRCVRDRTGELVCECKHNTAGPECDRCKPFHYDRPWQRATARE--ANECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGYYRDMSKPITHRKACKACDCHPVGAAGKT-CNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCIKIPSAPPTTTASITEQPADCDSYCKASKGKLKINMKKYCKKDYAVQVHILKSDKAGEWWKFTVNIVSVYKQSESRIRRGDQL--------LWVRAKDVACKCPKMKTGKKYLLLGNN--EDAPGQSGMVADKGSLVIQWRDTWARRLRKFQQREKKGKCKK 603
BLAST of Netrin 1 vs. Ensembl Cavefish
Match: NTN1 (netrin 1 [Source:HGNC Symbol;Acc:HGNC:8029]) HSP 1 Score: 318.546 bits (815), Expect = 4.713e-100 Identity = 194/586 (33.11%), Postives = 301/586 (51.37%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGR-IPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSL--LPNSRLIKEWVSVTDIMISL--LTDGSKKRNKSPYDHLSISDITIGARSQCYGHAKSLIKK--NGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPC-----APETKNNLMATEERCGSC------GKNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRIQQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKCDR 1837
C+ E G A+ C PDF+N A +STCG KG + R C G C CD P+ + S + D N + T W S + G ++ +T++L K +EV Y+ V+F + PDSM ++K GKTW+P+ ++S+ C+K F + Q N+ C + ++ + + F+ + GR S NS ++++WV+ TD+ ++ LT + ++SD+ +G R +C GHA +K+ G VCDC H TAGP+C +C H D+PW+ E + C C CN HA C+F+ ++YK S K G CL+C H+T G HC+ C GYYR+ + PI+H + C+ C CHP G+ ++ CN G C CK GV G CNRC KGYQQ++S PC AP T ++ E C K + +I +K+CKK +++ I + GEW +F V ++ +++ +S + + +WV D+ CKCP ++ G YLLL ++ Q ++ D+ S + W D W RRL F+ RE++GKC +
Sbjct: 37 CYDEHGAARRCIPDFVNAAFGKEVRASSTCGLKGPS-RYCAQDKGEEGART--CHTCDASDPKRAHPGSYLTDLNNPHNLTCWQSETGVQALQG--NVTLTLSLGKKFEVTYVSVQFCSKRPDSMAIFKSMDYGKTWVPFQFYSTQCRKVFNRPNRAAITKQ----NEQEAVCTDAHADGRPLSGGL--VAFSTLDGRPSAHDFDNSPVLQDWVTATDVRVTFSRLTAPGEPDEDEDSLFYAVSDLQVGGRCKCNGHASRCVKERDTGGLVCDCKHNTAGPECDRCKPFHYDRPWQRATARE--AHECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGYYRDLSKPISHRKACKACDCHPVGAAGKT-CNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCIKIPVAPPT--TTASSPEEPADCDSYCKASKGKLKINMKKYCKKDYAVQVHILKSDKAGEWWKFTVNIISVYKQGESRIRRGDQL--------LWVRAKDVACKCPKIKTGKKYLLLGND--EDSPGQSGVVADKGSLVIQWRDTWARRLRKFQQREKKGKCKK 596
BLAST of Netrin 1 vs. Ensembl Cavefish
Match: NTN1 (netrin 1 [Source:HGNC Symbol;Acc:HGNC:8029]) HSP 1 Score: 318.161 bits (814), Expect = 4.607e-98 Identity = 193/593 (32.55%), Postives = 303/593 (51.10%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGR-IPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIK---------VLPFNPMTGRTSL--LPNSRLIKEWVSVTDIMISL--LTDGSKKRNKSPYDHLSISDITIGARSQCYGHAKSLIKK--NGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPC-----APETKNNLMATE-ERCGS---CGKNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRIQQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKCDR 1837
C+ E G A+ C PDF+N A +STCG KG + R C G C CD P+ + S + D N + T W S + G ++ +T++L K +EV Y+ V+F + PDSM ++K GKTW+P+ ++S+ C ++ FN+ + A + ++ + + ++ ++ F+ + GR S NS ++++WV+ TD+ ++ LT + ++SD+ +G R +C GHA +K+ G VCDC H TAGP+C +C H D+PW+ E + C C CN HA C+F+ ++YK S K G CL+C H+T G HC+ C GYYR+ + PI+H + C+ C CHP G+ ++ CN G C CK GV G CNRC KGYQQ++S PC AP T E C S K + +I +K+CKK +++ I + GEW +F V ++ +++ +S + + +WV D+ CKCP ++ G YLLL ++ Q ++ D+ S + W D W RRL F+ RE++GKC +
Sbjct: 214 CYDEHGAARRCIPDFVNAAFGKEVRASSTCGLKGPS-RYCAQDKGEEGART--CHTCDASDPKRAHPGSYLTDLNNPHNLTCWQSETGVQALQG--NVTLTLSLGKKFEVTYVSVQFCSKRPDSMAIFKSMDYGKTWVPFQFYSTQC---------------RKVFNRPNRAAITKQNEQEAVCTDAHADGRPLSGGLVAFSTLDGRPSAHDFDNSPVLQDWVTATDVRVTFSRLTAPGEPDEDEDSLFYAVSDLQVGGRCKCNGHASRCVKERDTGGLVCDCKHNTAGPECDRCKPFHYDRPWQRATARE--AHECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGYYRDLSKPISHRKACKACDCHPVGAAGKT-CNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCIKIPVAPPTTTASSPEEPADCDSYCKASKGKLKINMKKYCKKDYAVQVHILKSDKAGEWWKFTVNIISVYKQGESRIRRGDQL--------LWVRAKDVACKCPKIKTGKKYLLLGND--EDSPGQSGVVADKGSLVIQWRDTWARRLRKFQQREKKGKCKK 773
BLAST of Netrin 1 vs. Ensembl Cavefish
Match: ntn1b (netrin-1-like [Source:NCBI gene;Acc:111191060]) HSP 1 Score: 297.745 bits (761), Expect = 8.906e-92 Identity = 187/588 (31.80%), Postives = 297/588 (50.51%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGK-CLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSL--LPNSRLIKEWVSVTDIMIS---LLTDGSKKRNKSPYDHLS----ISDITIGARSQCYGHAKSLIKK--NGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPC-----APETKNNLMATE-ERCGS---CGKNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRI-QQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKC 1831
C+ E+G+ + C PDF+N A +STCG + + R C T G + C CD P+ + + + D N + T W S + ++ +T++L K +EV Y+ ++F P+SM +YK GKTW+P+ ++S+ CKK F + Q N+ C ++ + + + F+ + GR S NS ++++WV+ TD+ ++ L T G + + S S +SD+ +G R +C GHA +K G VC+C H TAG +C +C H D+PW+ E N C C CN HA C+F+ ++YK S + G CL+C H+T G HC+ C GYYR+ I+H + C+ C CHP G+ ++ CN G C CK GV G CNRC KGYQQ++S PC A T A E C S K + ++ +K+CKK +++ + + GEW +F + ++ +++ + ++ L +WV D+ CKCP ++ G YLLL + Q ++ D+ S + W D W RRL F+ R+++GKC
Sbjct: 57 CYDENGQPRRCIPDFVNAAFGKEVRASSTCGAQTAA-RYC--VVTERGEERSRECHTCDAADPKKAHPAAYLTDLNNPHNLTCWQSESYVQHP--QNVTLTLSLGKKFEVTYVSLQFCSPRPESMAIYKSMDYGKTWVPFQFYSTQCKKMFNRPSRAAITKQ----NEQEAVCTDSHTDMRPLAGGL--IAFSTLDGRPSAHDFDNSPVLQDWVTATDVRVTFSRLHTFGDENEDDSELARDSYFYAVSDLQVGGRCKCNGHASKCVKDRDGGGLVCECKHNTAGAECDRCKPFHYDRPWQRATARE--ANECVACHCNLHARRCRFNMELYKLSGRRSGGVCLNCRHNTAGRHCHYCKEGYYRDMTKAISHRRACKACDCHPVGAAGKT-CNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCIKIPVASPTATYTSAEEPTDCDSHCKASKGKMKVTMKKYCKKDFAVQVHVLKGDKAGEWWKFTINIISVYKQGEHRIRRGDQL---------LWVRAKDVACKCPKIKPGKKYLLLGSDD-DDSRGQSGVVADKGSLLIPWKDLWARRLRKFQQRDKRGKC 620
BLAST of Netrin 1 vs. Ensembl Cavefish
Match: NTN1 (netrin 1 [Source:HGNC Symbol;Acc:HGNC:8029]) HSP 1 Score: 257.299 bits (656), Expect = 1.206e-75 Identity = 168/584 (28.77%), Postives = 276/584 (47.26%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGR-IPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIK---------VLPFNPMTGRTSL--LPNSRLIKEWVSVTDIMISL--LTDGSKKRNKSPYDHLSISDITIGARSQCYGHAKSLIKK--NGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPCAPETKNNLMATEERCGSCGKNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRIQQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKCDR 1837
C+ E G A+ C PDF+N A +STCG KG + R C G C CD P+ + S + D N + T W S + G ++ +T++L K +EV Y+ V+F + PDSM ++K GKTW+P+ ++S+ C ++ FN+ + A + ++ + + ++ ++ F+ + GR S NS ++++WV+ TD+ ++ LT + ++SD+ +G R +C GHA +K+ G VCDC H TAGP+C +C H D+PW+ E + C C CN HA C+F+ ++YK S K G CL+C H+T G HC+ C GYYR+ + PI+H + C++ P + S P + CK +GKL +I +K+CKK +++ I + GEW +F V ++ +++ +S + + +WV D+ CKCP ++ G YLLL ++ Q ++ D+ S + W D W RRL F+ RE++GKC +
Sbjct: 214 CYDEHGAARRCIPDFVNAAFGKEVRASSTCGLKGPS-RYCAQDKGEEGART--CHTCDASDPKRAHPGSYLTDLNNPHNLTCWQSETGVQALQG--NVTLTLSLGKKFEVTYVSVQFCSKRPDSMAIFKSMDYGKTWVPFQFYSTQC---------------RKVFNRPNRAAITKQNEQEAVCTDAHADGRPLSGGLVAFSTLDGRPSAHDFDNSPVLQDWVTATDVRVTFSRLTAPGEPDEDEDSLFYAVSDLQVGGRCKCNGHASRCVKERDTGGLVCDCKHNTAGPECDRCKPFHYDRPWQRATARE--AHECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGYYRDLSKPISHRKACKEIPVAPPTTTASSPEEPADCDSYCKAS-KGKL-----------------------------------------KINMKKYCKKDYAVQVHILKSDKAGEWWKFTVNIISVYKQGESRIRRGDQL--------LWVRAKDVACKCPKIKTGKKYLLLGND--EDSPGQSGVVADKGSLVIQWRDTWARRLRKFQQREKKGKCKK 723
BLAST of Netrin 1 vs. Ensembl Cavefish
Match: ntn1b (netrin-1-like [Source:NCBI gene;Acc:111191060]) HSP 1 Score: 236.113 bits (601), Expect = 3.776e-69 Identity = 161/579 (27.81%), Postives = 271/579 (46.80%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGK-CLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSL--LPNSRLIKEWVSVTDIMIS---LLTDGSKKRNKSPYDHLS----ISDITIGARSQCYGHAKSLIKK--NGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPCAPETKNNLMATEERCGSCGKNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRI-QQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKC 1831
C+ E+G+ + C PDF+N A +STCG + + R C T G + C CD P+ + + + D N + T W S + ++ +T++L K +EV Y+ ++F P+SM +YK GKTW+P+ ++S+ CKK F + Q N+ C ++ + + + F+ + GR S NS ++++WV+ TD+ ++ L T G + + S S +SD+ +G R +C GHA +K G VC+C H TAG +C +C H D+PW+ E N C C CN HA C+F+ ++YK S + G CL+C H+T G HC+ C GYYR+ I+H + C++ P S +Y + + T C K + K + ++ +K+CKK +++ + + GEW +F + ++ +++ + ++ L +WV D+ CKCP ++ G YLLL + Q ++ D+ S + W D W RRL F+ R+++GKC
Sbjct: 57 CYDENGQPRRCIPDFVNAAFGKEVRASSTCGAQTAA-RYC--VVTERGEERSRECHTCDAADPKKAHPAAYLTDLNNPHNLTCWQSESYVQHP--QNVTLTLSLGKKFEVTYVSLQFCSPRPESMAIYKSMDYGKTWVPFQFYSTQCKKMFNRPSRAAITKQ----NEQEAVCTDSHTDMRPLAGGL--IAFSTLDGRPSAHDFDNSPVLQDWVTATDVRVTFSRLHTFGDENEDDSELARDSYFYAVSDLQVGGRCKCNGHASKCVKDRDGGGLVCECKHNTAGAECDRCKPFHYDRPWQRATARE--ANECVACHCNLHARRCRFNMELYKLSGRRSGGVCLNCRHNTAGRHCHYCKEGYYRDMTKAISHRRACKEI---PVASPTATYTSAEE---------------------------PTDCDSHCKAS------------KGKMKVTMKKYCKKDFAVQVHVLKGDKAGEWWKFTINIISVYKQGEHRIRRGDQL---------LWVRAKDVACKCPKIKPGKKYLLLGSDD-DDSRGQSGVVADKGSLLIPWKDLWARRLRKFQQRDKRGKC 570
BLAST of Netrin 1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009682.1 (pep scaffold:Pmarinus_7.0:GL479835:738:11671:-1 gene:ENSPMAG00000008750.1 transcript:ENSPMAT00000009682.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 140.584 bits (353), Expect = 3.829e-37 Identity = 102/335 (30.45%), Postives = 162/335 (48.36%), Query Frame = 2
Query: 446 KLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSL--LPNSRLIKEWVSVTDIMISLL---TDGSKKRN-----KSPYDHLSISDITIGARSQCYGHAKSLIKKN-GTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPCAPETKNNLMATEE 1417
K +++ Y+ ++F P+S +YKR W P+ Y+S SC+ ++ + L ++ +P C + +T + F+ + GR S NS ++++WV+ T+I ++L T G + N +S Y +ISD +G R +C GHA +K G C C H T G DC +CL +ND+PW + + N C C CN A+EC FD ++Y+ + HG C +C +T G C C +YR A + C C C+P GS + C+ + G C CK GV G+ C++C G+ + C P T N E+
Sbjct: 2 KAFDITYVRLKFHTSRPESFAIYKRASMDGPWTPYQYYSGSCEPTYGKRNRGFLRSGD---DEQTPLCTDEFSDISPLTGG--NVAFSTLEGRPSAYNFDNSPVLQDWVTSTEIRVTLNRLNTFGDEVFNDHKVLRSYY--YAISDFAVGGRCKCNGHASECVKNELGALACKCRHNTMGADCEKCLPLYNDRPWHRA--TSESANECTLCDCNGRADECYFDPELYRATG--HGGHCTNCRDNTDGKTCERCLENFYRRSA-----SEHCLPCNCNPVGSYSRQ-CD-EQGRCSCKQGVMGERCDQCLPGFHSMDEAG--CRPCTCNPAGTLED 316
BLAST of Netrin 1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008925.1 (pep scaffold:Pmarinus_7.0:GL476496:30277:70426:-1 gene:ENSPMAG00000008054.1 transcript:ENSPMAT00000008925.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 104.375 bits (259), Expect = 2.123e-23 Identity = 114/477 (23.90%), Postives = 189/477 (39.62%), Query Frame = 2
Query: 446 KLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSLLPNSRLIKEWVSVTDIMI------SLLTDGSKKRNKSPYD---------HLSISDITIGARSQCYGHAKSLIKKNGT--TVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSG-------KHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQ------SYCNPDN----GICQCKIGVEGKLCNRCK---KGYQQTK---------SSSTPCAPETKNNLMATEERCGSC--GKNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRI--QQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLN 1726
++++V Y+IV+ A + +R +G T+ PW Y+S S + P + + + + + V + + K+ + + GR S S + ++ S I + +L D ++ P + + SI DI++G C GHA + T C C H T G +C C +N + W+ + GN CE+C C+ E+C +D ++ + + G C++C +T G +C +C G+YR N P C+ C C P GSL Q + +P+ G C C+ G G+ C RC +GY K ++ PC+ E C C G R R + C +S + + W V ++ WR+ +Q L+ E + + V NAD+R P L Y L L N L
Sbjct: 1 QVFQVAYVIVKAANSPRPGNWILERSLDGATYHPWQYYSVSDSECLTRYGITPT-VGNPVYRRDDEVICTSYYVVLLMLARGKIH-TSLINGRPSADDPSPKLLDFTSARYIRLRLQRIRTLNADLMTLSHRDPREVDPIVTRRYYYSIKDISVGGMCICSGHASTCPWNEDTQKMECQCEHNTCGENCQHCCPGYNQRRWRPGTINN--GNTCEKCNCHGKTEDCYYDAEVDRTNRSLSVHGRFSGGGVCVNCSANTAGTNCETCRDGFYRPTGVLPNDPYPCRLCQCDPQGSLSQVCIKDEKHADPEKDLSPGQCLCRPGFAGERCERCAFAYRGYPDCKPCLCSMAGGTNDDPCSEPCVCKERVEGEHCDRCRAGFYDLRPRNPRGCSACFCFGLSSSCRSL--PWGVTQVVDMRGWRVTDRQGLRKVKTFVE----VDQVAVRNADVRRTLPALY----YWLAPTSYLGNKLT 463 HSP 2 Score: 61.6178 bits (148), Expect = 4.692e-10 Identity = 57/177 (32.20%), Postives = 76/177 (42.94%), Query Frame = 2
Query: 914 GTTVCD-CLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGS-LPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKS----SSTPCAP-ETKNNLMATEERC 1423
G CD CL G C +C +P S P G+ C RC CN + EE ++ +G+ CL C T G HC C+ G+Y + CQ C CHP G+ PQ C+ NG C C+ V G+ C +C G+ S S PC P T + + RC
Sbjct: 700 GVLTCDQCLPGYIGLRCERCADDFFGEP------SSPGGS-CRRCECNGN-EEAWGGGRVCDARTGQ----CLRCRERTAGFHCERCADGFYGD----ATGTGGCQPCQCHPEGATAPQ--CDRINGQCPCRPSVVGRTCEQCAIGFYGLSSGAGCSPCPCHPVGTAGVACSADGRC 858 HSP 3 Score: 51.9878 bits (123), Expect = 3.954e-7 Identity = 48/173 (27.75%), Postives = 71/173 (41.04%), Query Frame = 2
Query: 680 TSNIKVLPFNPMTGRTSLLPNSR--LIKEWVSVTDIMISLLTDGSKKRNKSPYDHLSISDITIGARSQCYGHAKSLIKKNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRN 1192
T ++++LP N R++ P SR L+ +VT + I S K LS + + + G +L + C C H AG C C++ H + G CE C C+ HA++C + SG C H+T GPHC C PG+Y N
Sbjct: 517 TLSLRLLPENFFDFRSNA-PVSRDALMTALANVTRLQIR----ASYSSVKQAVYRLSAVSLDVASPDAAVGSPAALDVEQ----CHCPHGYAGTSCESCMRGHRRVDGTL------HGGRCEPCRCHGHADDCD-------DLSGDCMLPLSGCRHNTMGPHCELCRPGFYGN 667
BLAST of Netrin 1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004606.1 (pep scaffold:Pmarinus_7.0:GL477874:5526:15188:-1 gene:ENSPMAG00000004198.1 transcript:ENSPMAT00000004606.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 86.2705 bits (212), Expect = 1.341e-19 Identity = 58/197 (29.44%), Postives = 96/197 (48.73%), Query Frame = 2
Query: 443 NKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSLLP---NSRLIKEWVSVTDIMISL-----LTD---GSKKRNKSPYDHLSISDITIGARSQCYGHAKSLIK-KNGTTVCDCLHKTAGPDCSQCLKTHNDQPW 997
K +++ Y+ + F P+S +YKR W P+ ++S +C+++F + L + FS + +T + F+ + GR L N +L +EWV+ TD+ +SL D G K +S Y ++SD+ +G R +C+GHA + +G VC C H T G DC +CL +HN +PW
Sbjct: 3 GKAFQITYVRLRFHTSRPESFAIYKRTSPASPWQPYQFYSGTCERTFGLPSRGFLRAGDEERTALCSDEFS---DISPLTGG--NVAFSTLEGRPGALAFDGNDKLQQEWVTATDVRVSLRRLNTFGDEVFGDPKVLRSYY--YAVSDLAVGGRCKCHGHASECGRGSDGRLVCRCQHNTTGDDCERCLPSHNSRPW 192
BLAST of Netrin 1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004614.1 (pep scaffold:Pmarinus_7.0:GL477874:5547:15173:-1 gene:ENSPMAG00000004198.1 transcript:ENSPMAT00000004614.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 79.7221 bits (195), Expect = 1.485e-17 Identity = 55/202 (27.23%), Postives = 89/202 (44.06%), Query Frame = 2
Query: 452 YEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRT-----------SLLPNSRLIKEWVSVTDIMISL-----LTD---GSKKRNKSPYDHLSISDITIGARSQCYGHAKSLIK-KNGTTVCDCLHKTAGPDCSQCLKTHNDQPW 997
+++ Y+ + F P+S +YKR W P+ ++S +C +R+F S + + + +P+TG S P S +EWV+ TD+ +SL D G K +S Y ++SD+ +G R +C+GHA + +G VC C H T G DC +CL +HN +PW
Sbjct: 1 FQITYVRLRFHTSRPESFAIYKRTSPASPWQPYQFYSGTC---------------ERTFGLPSRGFLRAGDEERTALCSDEFSDISPLTGGNVAFSTLEGLFHSTGPLSSATQEWVTATDVRVSLRRLNTFGDEVFGDPKVLRSYY--YAVSDLAVGGRCKCHGHASECGRGSDGRLVCRCQHNTTGDDCERCLPSHNSRPW 185
BLAST of Netrin 1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007784.1 (pep scaffold:Pmarinus_7.0:GL477336:382472:417638:1 gene:ENSPMAG00000007035.1 transcript:ENSPMAT00000007784.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 85.8853 bits (211), Expect = 1.613e-17 Identity = 79/307 (25.73%), Postives = 126/307 (41.04%), Query Frame = 2
Query: 305 DDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHS-INITINL-NKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSLLPN------SRLIKEWVSVTDIMISLL-----TDGSKKRNKSPYDHLSISDITIGARSQCYGHAKSLIKKNGTTVCDCLHK--TAGPDCSQCLKTHNDQPWKIIFPSEPAG-----NICERCFCNNHAEECKFDEKIYKNSSGKH----GSRCLHCLHHTTG 1153
DDR+P +S + + N TVWVS P + ++I L Y+V+Y+IV+F P+ + + +R G W+ W F+ C + M ++ PL + S N C F V N+ F+ +T +L P + ++ +V ++ + L G PY ++S++ + R +C+GHA+ C C + T GP+C C D+PW+ AG N C C C A C++ E I G H G C C +TTG
Sbjct: 67 DDRVPRVSPHARPLSHVNDDDASTVWVSAPSPGPGQPPPSVAVSIELRGGSYQVFYVIVQFLSPQPEGVRIQRRPAAGSPWVDWQVFAKDCVSMYNMSKNGPLE-KPDSVN-----CLQFPREVPLSRGNVT---FSVLTPEPNLRPGYNAFYATPALQAFVLAAEVRVLLEGRLLGVAGVAGGPLRPYH--AVSEVIVSGRCECHGHAERCDSSTTPYRCLCSAQSNTEGPNCELCRPLFRDKPWR-------AGDTRNQNACRPCLCYGRALSCRY-EAILDPHPGLHWRGGGGVCQDCTQNTTG 354
BLAST of Netrin 1 vs. Ensembl Nematostella
Match: EDO36836 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SHI5]) HSP 1 Score: 253.062 bits (645), Expect = 4.594e-76 Identity = 166/581 (28.57%), Postives = 290/581 (49.91%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGR--TSLLPNSRLIKEWVSVTDIMISLLTDGSKKRNKSPYDHL--SISDITIGARSQCYGHAKSLIK-KNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPCAPETKNNLMATE--------ERCGSCGKNRPRIRFRKFCKKHIMIKISI--QSKEIHGEWTRFGVRVLKIWRIQQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKC 1831
C++E RA+ C+P F ++A N V+ TCG+K + R C S C CD+R + S + D + + + T W + + ++ + ++ K Y++ Y+ ++F G PDSM +YK GK+W + ++SS C+K + + + + A + H ++ + + F+ + GR S + S +++EWV TDI + R+ P D L ++S+ +G R C GHA + +NG VCDC H T G DC C ND+PW+ + + N C+ C CN HA C F +++K S G+ G C+ C H+T G +C+ C G+Y++ I H +VC++C CHP+GS + CN G C CK GV G+ C++C GY+ TKS+ PC ET N +M+ E + C C R ++ ++FC+K +K+++ K++ W R V + +++ ++ + + Y +W++ D RC+CP L+ G +Y + L+ + ++++ +S L W++ ++R+ F + C
Sbjct: 5 CYNEFNRAQLCQPAFHDLAYNKDVYVSHTCGEK--SRRFCP---PMSVRGRTACGFCDERNAKTSHPAKYLTDVHNENNVTCWQADPV---QSRENVTLILSFGKTYDITYVSLQFCGIRPDSMGIYKSMDYGKSWQAYQFYSSRCEKMYGRVTNGEISPENEQV-----ALCTDGHLLRPLYGG--RIAFSTLEGRPSASKMDYSAVMQEWVMATDIKVEF---NMISRSARPRDSLYYAVSEFNVGGRCHCNGHASECVSDRNGRQVCDCKHNTVGVDCGACRPFFNDRPWRPA--THHSANECKMCNCNLHARTCVFSMELFKLSGGRSGGVCVKCRHNTAGRYCHYCREGFYKDPNKHITHIKVCRQCNCHPHGSRGR-VCNQSTGQCPCKEGVVGRSCDKCAPGYKSTKSNIVPCI-ETPNVVMSQESSTPRSRDQGCSQC--RRVPLKVKRFCRKDFALKVTVVRVEKKLPNSWVRATVNINTLFKRSRTRRVRRG---DGY----VWIKKRDFRCRCPKLKPGRSYFIAG--NLRGQSRKRSIVLNGRSVVLPWNEKLLKRIYRFIKLQRNNNC 552
BLAST of Netrin 1 vs. Ensembl Nematostella
Match: EDO36523 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SIF3]) HSP 1 Score: 160.999 bits (406), Expect = 3.531e-41 Identity = 126/422 (29.86%), Postives = 197/422 (46.68%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNE--GKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSL--LPNSRLIKEWVSVTDIMISL-----LTD---GSKKRNKSPYDHLSISDITIGARSQCYGHAKSLIKK---NGTT--VCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGY 1348
C+ GRA+ C P F N A +TCG + + +G+ E +C C+ P L + D + T W S + + + + +T++L K Y+V YI + F P+SM +YK+ W+P+ YFS++C+K++ + + + + N+ C ++ +T + F+ + R NS ++EWV+ + I L D G +S Y +I D++IG R +C GHA + +G T C C H T G DC +C +ND+PW S N C RC CN AE C FDE +Y+ + HG RC +C +T GP+C C +YR + + C CGC+P GS+ CN + G C+CK GV G C++C+ Y
Sbjct: 21 CYDSQGRAQRCMPQFGNAAFGKRVISNNTCGDPP------KKFCVQTGVLEVRKECEMCNSSDPALEHPALKITDIKDDTNRTWWQSETLLE-NPDKPVTLTLDLGKSYDVSYIRIRFRSPRPESMAIYKKTTPSSKWIPYQYFSATCEKTYGVTTNGIVDPR----NQQVALCTDAFSTITPLTGG--NVAFSTLEDRPDAYNFDNSPALQEWVTAVAVRIHLRRMNTFGDEVFGDPNVLRSYY--YAIIDLSIGGRCKCNGHASECEETRLWDGQTRLQCKCQHDTEGVDCERCKPLYNDRPW--ARASSRNANECVRCNCNGLAESCVFDEALYRQTG--HGGRCQNCKQNTDGPNCERCKENFYRKTSL-----EPCVACGCNPVGSVSLQ-CNSE-GQCRCKPGVTGIKCDKCQANY 416
BLAST of Netrin 1 vs. Ensembl Nematostella
Match: EDO36501 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SIF6]) HSP 1 Score: 152.525 bits (384), Expect = 2.247e-38 Identity = 121/411 (29.44%), Postives = 189/411 (45.99%), Query Frame = 2
Query: 167 EPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVS-------GRIPKFSGIHSINITINLNKLYEVY-YIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKV-LPFNPMTGRTSLLPNSRLIKEWVSVTDIMISLL---TDG---SKKRNKSPYDHLSISDITIGARSQCYGHAK--SLIKKNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGY 1348
EP F ++ TSTCG S CR LN C KC+ P I + D++ T W S + P+ +N++I+ NK Y++ +I+ F P +L K G+TW P Y+S +C+K F M+ + P + F S C + K+S + N KV F + S +++++S TD+ + + TDG +K + ++SD+ + AR +C+GHA+ + G CDCLH T G DC +CL N++ W + SE N CERC C++HA+ C + ++ + C C H+T G C C +YRN + +NHP++C C C P G CN G C+C+ V G+ C+ C +
Sbjct: 35 EPRFAAKMKHRAVSSTSTCGSPPS--EYCR----VKPLN--GCFKCNASSPTERHPIEYIADKSPGSDATFWQSETWWDWYQKNPRDD--LKVNVSISFNKTYDITGHIMFTFESPRPSKAILEKSFDKGQTWDPLQYYSFNCRKDFGMENTDPT---ENRFGDFSAKC-TEKYSSSSPQQNGKVTFDFLRRYNKASFFDTQ--VQQYLSATDLRLRMEYPGTDGLEVAKDEVTLKQYYYAVSDLEVIARCKCHGHARWCDFPHETGKN-CDCLHNTTGDDCERCLPLFNNKTW-MPATSETEPNPCERCECHSHADSCTYSQQ-------EGYGTCDDCYHNTEGHFCERCKDKFYRNMSLSLNHPEICPDCNCFPQGITNNGTCNQTTGQCECRANVTGRQCDSCADTF 420
BLAST of Netrin 1 vs. Ensembl Nematostella
Match: EDO39502 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S9X8]) HSP 1 Score: 120.168 bits (300), Expect = 5.420e-28 Identity = 91/338 (26.92%), Postives = 149/338 (44.08%), Query Frame = 2
Query: 407 SGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSLL-PNSRLIKEWVSVTDIMIS---LLTDGSKKRNKSPYD----HLSISDITIGARSQCYGHAKSLIKKNGTTV--------CDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDN--------GICQCKIGVEGKLCNRCKKGY 1348
+G ++ + ++L + ++I+ F P MV+ + GK W + YFS+ C K F I + I + K+S ++N +V+ F + + + S ++ + +T+I I+ L T G + P + ++ D+ I CYGHA+ + + G C+C H T G +C +C + D PW+ P N C +C CN HA +C+FD +Y+ S G C C H+T G +C C P +Y++ + + P C C C P G + C N G C CK V G C+RCK G+
Sbjct: 102 NGRENVYLQLDLESEFHFTHLIMRFRTFRPAGMVIERSWDYGKKWQIYRYFSADCDKIFP-------GIPKGEPKDIDDVICTNKYSTVEPSTNGEVI-FRALKSQIPIKNAYSSKVQNLLKITNIRINFTKLHTLGDDLLDPRPEIKRKYYYAVYDLVIRGSCSCYGHAEQCLPEPGQPTTPGMVYGRCNCTHNTTGKNCERCQDGYFDVPWRPADADNP--NECRKCNCNGHAVKCRFDPAVYQASGNVSGGVCQDCEHNTVGRNCERCKPLHYQDPSKKFSDPDACIACNCDPTGGVNGGECEAKNDPAAGLVAGRCICKRNVGGVRCDRCKAGF 429 HSP 2 Score: 64.3142 bits (155), Expect = 1.181e-10 Identity = 60/210 (28.57%), Postives = 80/210 (38.10%), Query Frame = 2
Query: 875 QCYGHAKSLIKKNGTTVCDCLHKTAGPDCSQCL---------KTHND----------------QPWKIIFPSEPAGNICERC---FCNNHAEECK----------------------FDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSY--CNPDNGICQCKIGVEGKLCNRCKKGY 1348
+CYGHA S K G V DC H T G +C C T ND + + + P +C++C + EEC D + N GS CL CL++T G +C C PGY+ + A N C C C G+ P + CN G C C VEGK C+RC +G+
Sbjct: 851 RCYGHASSCNPKTGVCV-DCQHNTVGKNCEACAVGFYGIATKGTPNDCKRCQCPGGSSGNKFSESCYVDTSTTPPSVLCDKCQVGYTGRQCEECADGYYGNPLVPGGSCKPCSCNNNIDPTVSGNCDRLTGS-CLQCLYNTAGFNCERCKPGYFGD-AIARN----CTSCVCDKKGTNPGFHYSCNHKTGQCNCLPDVEGKQCDRCAEGF 1053
BLAST of Netrin 1 vs. Ensembl Nematostella
Match: EDO46760 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RNY3]) HSP 1 Score: 113.235 bits (282), Expect = 8.382e-26 Identity = 99/376 (26.33%), Postives = 174/376 (46.28%), Query Frame = 2
Query: 425 NITINLNKLYE---VYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKIS-PACFSFKHSVQNITSNIKVLPFNPMTGRTSLLPNSRLIKEWVSVTDIMISLLTDGSKKRNKSPYDHLSISDITIGARSQCYGHAK--SLIKKNGTTV------CDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKH---GSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCH-PYGSLPQSYCNPDN-------GICQCKIGVEGKLCNRCKKGYQQTKSSSTPCAPETKNNLMATEERCGSCGKNRPRIRFRKFCKKHI 1483
N T+ ++ LY+ ++++I+ F G P+++++ + G T+ PW YF+S C+ SF M+ + L + S N I C H+ Q +T IK +P R P++ E +++ I L + K Y ++ + + R +C+GHA+ + IK N ++ CD T G C CL +N++ + +P C +C CNNHA C ++ + + + + G C++CLH+T G C+ C ++R ++ VC C C P ++ C D+ G C+CK +G+ C+ CK G+ KSS + N T R SC ++R + +CK ++
Sbjct: 353 NATVEVDLLYQELQIFFVIMLFYGPPPEAILIERSRDRGSTYKPWQYFASDCQSSFGMENNGNL-VNPDSINCIQYQRCECHGHA-QKVTF-IKGNSSSPGRCRCECDPSTFTQGE--KASNVRIQLYNHTLIQNEKHRY--FALKRVLVTGRCECHGHAQKVTFIKGNSSSPGRCRCECDPSTFTQGEKCESCLPMYNNKAFLRGTNDDPYP--CVKCECNNHATACYYNSSLDPSPASRTVGGGGVCINCLHNTAGQFCHVCRDDFFREPGKSLSAVDVCSPCQCTGPGVETGKTSCARDDSTPGVIAGQCECKQYTQGRQCDACKPGFYDLKSSHSTGCVTCGCNTAGTVNRNASCDQSRGQC----YCKSNV 715 HSP 2 Score: 78.9518 bits (193), Expect = 4.006e-15 Identity = 53/185 (28.65%), Postives = 79/185 (42.70%), Query Frame = 2
Query: 842 SISDITIGARSQCYG----HAKSLIKKNGTT------VCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSS 1366
S+S + + + QC G K+ ++ +T C+C T G C C D + S G C C CN + +N+S +C + TG C++C G+Y R N P C+ C C P+G+LP S C+ +G C+CK G G C+ C+ GY SS
Sbjct: 618 SLSAVDVCSPCQCTGPGVETGKTSCARDDSTPGVIAGQCECKQYTQGRQCDACKPGFYD-----LKSSHSTG--CVTCGCNTAG-------TVNRNASCDQSRGQCYCKSNVTGRTCDTCESGFYGLRD---NDPSGCRACECDPFGTLPGSVCDAGSGQCRCKAGSTGLRCHLCEDGYHSLDSS 785 HSP 3 Score: 67.0106 bits (162), Expect = 2.267e-11 Identity = 52/173 (30.06%), Postives = 68/173 (39.31%), Query Frame = 2
Query: 926 CDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHA----EECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPCAPETKNNLMATEERCGSC 1432
C C P C QC+K + + + S P G C+ C CN + E S+GK CL + G C+ C YY N P + C C C GSL + CN G CQCK + G+ C+RC G S PC +L T + G C
Sbjct: 865 CTCKKYVREPTCGQCMKGYFN-----LSSSNPEG--CQACNCNPKGTLGGDRTPAGELECSGSNGKCA-----CLTNVQGLRCDQCVSAYYWN---PSGYG--CSPCNCDASGSLATN-CN-STGYCQCKPNIGGRRCDRCMPGSYGGPGSCKPCNCNMAGSLSDTCDDSGKC 1018 HSP 4 Score: 60.4622 bits (145), Expect = 2.372e-9 Identity = 43/150 (28.67%), Postives = 59/150 (39.33%), Query Frame = 2
Query: 926 CDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRC-----LHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTK 1360
C CL G C QC+ + W P+G C C C+ +SG + C C + G C+ C PG Y P C+ C C+ GSL + C+ D+G C CK V+G C+ CK G Q +
Sbjct: 924 CACLTNVQGLRCDQCVSAYY---WN------PSGYGCSPCNCD---------------ASGSLATNCNSTGYCQCKPNIGGRRCDRCMPGSY-------GGPGSCKPCNCNMAGSLSDT-CD-DSGKCNCKPNVQGDKCDTCKDGTQNLQ 1040
BLAST of Netrin 1 vs. Ensembl Medaka
Match: NTN1 (netrin-1 [Source:NCBI gene;Acc:101155405]) HSP 1 Score: 320.857 bits (821), Expect = 6.447e-101 Identity = 194/588 (32.99%), Postives = 306/588 (52.04%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGK-CLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSL--LPNSRLIKEWVSVTDIMI---SLLTDGSKKRNKSPYDH----LSISDITIGARSQCYGHAKSLIK-KNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPC------APETKNNLMATEERCGS---CGKNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRIQQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKCDR 1837
C+ E G + C PDF+N A +V+STCG+ S R C G + C CD P+ + + D N + T W S ++ ++ +T++L K +EV Y+ ++F P+SMV+YK NGKTW+P+ ++S+ C+K + + Q N+ C + +T + + F+ + GR S NS ++++WV+ TDI + L T G + + S ++SD+ +G R +C GHA +K ++G+ VCDC H TAGP+C +C H D+PW+ E N C C CN HA C+F+ ++YK S K G CL+C H+T G HC+ C G+YR+ + PI+H + C+ C CHP G+ ++ CN G C CK GV G CNRC KGYQQ++S PC P T + C S K + +I +K+CKK +++ I + GEW +F V ++ +++ +S + + + +WV D+ CKCP ++ G YLLL ++ +Q ++ D+ S + W D W RRL F+ RE++GKC +
Sbjct: 38 CYDEHGNPRRCIPDFVNAAFGKEVKVSSTCGKTPS--RYCVVTSAEKGEERTRNCHSCDASDPKKYHPPAYLTDLNNPHNLTCWQSENFIQYP--QNVTLTLSLGKKFEVTYVSLQFCSPRPESMVIYKSMDNGKTWVPFQFYSTQCRKMYNKPNRAVITKQ----NEQEAVCTDSHTDMYPLTGGL--IAFSTLDGRPSAHDFDNSPVLQDWVTATDIKVVFSRLHTFGDENEDDSELARDSYFYAVSDLQVGGRCKCNGHASRCVKDRDGSLVCDCKHNTAGPECDRCKPFHYDRPWQRATARE--ANECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGFYRDLSKPISHRKACKACDCHPVGAAGKT-CNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCIKIPVSPPTTPASSTEEASDCESYCKASKGKLKINMKKYCKKDYAVQVHILKADKMGEWWKFTVNIISVYKQGESRIRRGDQF--------LWVRAKDVACKCPKIKPGKKYLLLGNDEVSP--SQSGVVADKGSLVIQWRDTWARRLRKFQQREKKGKCKK 602
BLAST of Netrin 1 vs. Ensembl Medaka
Match: NTN1 (netrin-1 [Source:NCBI gene;Acc:101155405]) HSP 1 Score: 313.153 bits (801), Expect = 3.767e-98 Identity = 189/587 (32.20%), Postives = 300/587 (51.11%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSL--LPNSRLIKEWVSVTDIMI---SLLTDGSKKRNKSPYDH----LSISDITIGARSQCYGHAKSLIK-KNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPC------APETKNNLMATEERCGS---CGKNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRIQQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKCDR 1837
C+ E G + C PDF+N A +V+STCG+ CD P+ + + D N + T W S ++ ++ +T++L K +EV Y+ ++F P+SMV+YK NGKTW+P+ ++S+ C+K + + Q N+ C + +T + + F+ + GR S NS ++++WV+ TDI + L T G + + S ++SD+ +G R +C GHA +K ++G+ VCDC H TAGP+C +C H D+PW+ E N C C CN HA C+F+ ++YK S K G CL+C H+T G HC+ C G+YR+ + PI+H + C+ C CHP G+ ++ CN G C CK GV G CNRC KGYQQ++S PC P T + C S K + +I +K+CKK +++ I + GEW +F V ++ +++ +S + + + +WV D+ CKCP ++ G YLLL ++ +Q ++ D+ S + W D W RRL F+ RE++GKC +
Sbjct: 38 CYDEHGNPRRCIPDFVNAAFGKEVKVSSTCGKT-----------------------CDASDPKKYHPPAYLTDLNNPHNLTCWQSENFIQYP--QNVTLTLSLGKKFEVTYVSLQFCSPRPESMVIYKSMDNGKTWVPFQFYSTQCRKMYNKPNRAVITKQ----NEQEAVCTDSHTDMYPLTGGL--IAFSTLDGRPSAHDFDNSPVLQDWVTATDIKVVFSRLHTFGDENEDDSELARDSYFYAVSDLQVGGRCKCNGHASRCVKDRDGSLVCDCKHNTAGPECDRCKPFHYDRPWQRATARE--ANECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGFYRDLSKPISHRKACKACDCHPVGAAGKT-CNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCIKIPVSPPTTPASSTEEASDCESYCKASKGKLKINMKKYCKKDYAVQVHILKADKMGEWWKFTVNIISVYKQGESRIRRGDQF--------LWVRAKDVACKCPKIKPGKKYLLLGNDEVSP--SQSGVVADKGSLVIQWRDTWARRLRKFQQREKKGKCKK 580
BLAST of Netrin 1 vs. Ensembl Medaka
Match: ntn1b (netrin-1 [Source:NCBI gene;Acc:101161061]) HSP 1 Score: 298.901 bits (764), Expect = 1.869e-92 Identity = 185/587 (31.52%), Postives = 300/587 (51.11%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGK-CLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSL--LPNSRLIKEWVSVTDIMI---SLLTDGSKKRNKSPYDHLS----ISDITIGARSQCYGHAKSLIK-KNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPC------APETKNNLMATEERCGS---CGKNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRI-QQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKC 1831
C+ E+G + C PDF+N A V+STCG + R C + G + C CD P+ + + + D N + T W S ++ ++ +T++L K +EV Y+ ++F P+SM +YK GK+W+P+ ++S+ CKK + + + Q N+ C + +T + + F+ + GR S NS ++++WV+ TDI + L T G + + S S +SD+ +G R +C GHA +K + G VC+C H TAGP+C +C H D+PW+ E N C C CN HA C+F+ ++YK S + G CL+C H+T G HC+ C GY+R+ + PI+H + C+ C CHP G+ ++ CN G C CK GV G CNRC KGYQQ++S PC +P + + C S K + +I +K+CKK +++ + + GEW +F V ++ +++ + ++ L +WV D+ C+CP ++ G YLLL + ++ D+ S + W D W RRL F+ R+++GKC
Sbjct: 41 CYDENGNPRRCIPDFVNSAFGKDVRVSSTCGNPAA--RFC--VVSEKGEERTRDCHTCDSNDPKKAHPPAYLTDLNNPHNLTCWQSETYVQYP--QNVTLTLSLGKKFEVTYVSLQFCSPRPESMAIYKSMDYGKSWVPFQFYSTQCKKMYNRQNKATITKQ----NEQEAICTDSHTDMHPLTGGL--IAFSTLDGRPSAHDFDNSPVLQDWVTATDIKVIFSRLHTFGDENEDDSELARDSYFYAVSDLQVGGRCKCNGHASKCVKDREGNLVCECKHNTAGPECDRCKPFHYDRPWQRATARE--ANECVACHCNLHARRCRFNMELYKLSGRRSGGVCLNCRHNTAGRHCHYCKEGYFRDMSKPISHRRACKACDCHPVGAAGKT-CNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCIKIPVASPTSTYSSYEEPSDCDSYCKASKGKMKITMKKYCKKDYAVQVHVLKGDKAGEWWKFTVNIISVYKQGRHRIRRGDQL---------LWVRAKDVACRCPKIKPGRKYLLLGTD--DDSPGHSGVVADKGSLLIPWKDLWGRRLRKFQQRDKRGKC 601
BLAST of Netrin 1 vs. Ensembl Medaka
Match: ntn1b (netrin-1 [Source:NCBI gene;Acc:101161061]) HSP 1 Score: 298.516 bits (763), Expect = 2.090e-92 Identity = 185/587 (31.52%), Postives = 300/587 (51.11%), Query Frame = 2
Query: 134 CFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGK-CLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSL--LPNSRLIKEWVSVTDIMI---SLLTDGSKKRNKSPYDHLS----ISDITIGARSQCYGHAKSLIK-KNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPC------APETKNNLMATEERCGS---CGKNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRI-QQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKC 1831
C+ E+G + C PDF+N A V+STCG + R C + G + C CD P+ + + + D N + T W S ++ ++ +T++L K +EV Y+ ++F P+SM +YK GK+W+P+ ++S+ CKK + + + Q N+ C + +T + + F+ + GR S NS ++++WV+ TDI + L T G + + S S +SD+ +G R +C GHA +K + G VC+C H TAGP+C +C H D+PW+ E N C C CN HA C+F+ ++YK S + G CL+C H+T G HC+ C GY+R+ + PI+H + C+ C CHP G+ ++ CN G C CK GV G CNRC KGYQQ++S PC +P + + C S K + +I +K+CKK +++ + + GEW +F V ++ +++ + ++ L +WV D+ C+CP ++ G YLLL + ++ D+ S + W D W RRL F+ R+++GKC
Sbjct: 40 CYDENGNPRRCIPDFVNSAFGKDVRVSSTCGNPAA--RFCV--VSEKGEERTRDCHTCDSNDPKKAHPPAYLTDLNNPHNLTCWQSETYVQYP--QNVTLTLSLGKKFEVTYVSLQFCSPRPESMAIYKSMDYGKSWVPFQFYSTQCKKMYNRQNKATITKQ----NEQEAICTDSHTDMHPLTGGL--IAFSTLDGRPSAHDFDNSPVLQDWVTATDIKVIFSRLHTFGDENEDDSELARDSYFYAVSDLQVGGRCKCNGHASKCVKDREGNLVCECKHNTAGPECDRCKPFHYDRPWQRATARE--ANECVACHCNLHARRCRFNMELYKLSGRRSGGVCLNCRHNTAGRHCHYCKEGYFRDMSKPISHRRACKACDCHPVGAAGKT-CNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCIKIPVASPTSTYSSYEEPSDCDSYCKASKGKMKITMKKYCKKDYAVQVHVLKGDKAGEWWKFTVNIISVYKQGRHRIRRGDQL---------LWVRAKDVACRCPKIKPGRKYLLLGTD--DDSPGHSGVVADKGSLLIPWKDLWGRRLRKFQQRDKRGKC 600
BLAST of Netrin 1 vs. Ensembl Medaka
Match: ntn2 (netrin-3 [Source:NCBI gene;Acc:101160359]) HSP 1 Score: 289.656 bits (740), Expect = 1.210e-88 Identity = 190/633 (30.02%), Postives = 305/633 (48.18%), Query Frame = 2
Query: 125 FSSCFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGK-------CLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIK---------VLPFNPMTGRTSL--LPNSRLIKEWVSVTDIMIS----------LLTDGSKK--RNKSPYDHLS--------ISDITIGARSQCYGHAKSLIK-KNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPCAPETKNN---LMATEERCGSCG------KNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRIQ-QSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNY-------------LNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKCDR 1837
C+ E+G A+ C P+FIN A V+S CG+ S R C S + G C CD P + S + D N + T W S + + H++ +T++L K +E+ Y+ ++F P+S+ +YK GKTW+P+ ++SS C +R +NK + A + ++ + + ++ ++ F+ + GR S NS ++++WV+VTDI + +L GS R+ P S + D +G R +C GHA +K K G VCDC H T GP+C +C H D+PW+ E N C C CN HA C+F+ ++YK S K G C++C H+T G HC+ C G+YR+ PI H + C+ C CHP G+ ++ CN G C CK GV G CNRC KGYQQ++S PC N ++++ E C K +I +K+CKK +++++ E G+W +F V ++ +++ + + LK N+ +WV D+ CKCP +Q+ +L++ + + L+ D+ S + W D W RRL F+ +E++GKC +
Sbjct: 34 LDPCYDETGTARRCIPEFINAAFGKEVMVSSVCGRPPS--RSC------SVVERGDERPSVRTCQICDAADPRRAHPASYLTDLNSAHNLTCWQSENL--NTSPHNVTLTLSLGKKFEITYVSLQFCSPRPESLAIYKSMDYGKTWMPYQFYSSQC---------------RRMYNKPNKATITKQNEQEALCTDGHTDLYPLSGGLIAFSTLDGRPSSKDFDNSPVLQDWVTVTDIRVVFGRPQLSREFVLGAGSNNGGRDDDPVAVTSTLSTYFYAVGDFQVGGRCKCNGHASRCLKDKEGKLVCDCKHNTEGPECDRCKPFHYDRPWQRANARE--ANECLPCNCNLHARRCRFNMELYKLSGRKSGGVCMNCRHNTAGRHCHYCKEGFYRDMGRPITHRRACKACDCHPVGAAGKT-CNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPVAPCIKIPVVNPTAVVSSTEEPADCESYCKPVKGNLKINMKKYCKKDYAVQVNVLDMETVGDWAKFSVSLVSVYKSRGEPLKRGDNI---------LWVHMKDLACKCPKIQMSKKFLVMGGSEGGTGPAAGPGVGATSPGAERVGLVADKNSLVIQWRDVWSRRLRKFQRKEKKGKCSK 629
BLAST of Netrin 1 vs. Planmine SMEST
Match: SMESG000006779.1 (SMESG000006779.1) HSP 1 Score: 1186.4 bits (3068), Expect = 0.000e+0 Identity = 586/586 (100.00%), Postives = 586/586 (100.00%), Query Frame = 2
Query: 122 GFSSCFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSLLPNSRLIKEWVSVTDIMISLLTDGSKKRNKSPYDHLSISDITIGARSQCYGHAKSLIKKNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPCAPETKNNLMATEERCGSCGKNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRIQQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKCDRYQYKRKHSKLMNSR 1879
GFSSCFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFSSSCKKSFKMKRSKPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSLLPNSRLIKEWVSVTDIMISLLTDGSKKRNKSPYDHLSISDITIGARSQCYGHAKSLIKKNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPCAPETKNNLMATEERCGSCGKNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRIQQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKCDRYQYKRKHSKLMNSR
Sbjct: 22 GFSSCFSESGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFSSSCKKSFKMKRSKPLHIQQRSFNKISPACFSFKHSVQNITSNIKVLPFNPMTGRTSLLPNSRLIKEWVSVTDIMISLLTDGSKKRNKSPYDHLSISDITIGARSQCYGHAKSLIKKNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPCAPETKNNLMATEERCGSCGKNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRIQQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKCDRYQYKRKHSKLMNSR 607
BLAST of Netrin 1 vs. Planmine SMEST
Match: SMESG000050895.1 (SMESG000050895.1) HSP 1 Score: 385.185 bits (988), Expect = 1.021e-124 Identity = 215/606 (35.48%), Postives = 327/606 (53.96%), Query Frame = 2
Query: 146 SGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGK-CLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFK--HSVQNITSNIKVLPFNPMTGRTSLLPNSRLIKEWVSVTDIMISLLTDGSKK-------------------------RNKSPYDHLSISDITIGARSQCYGHA-KSLIKKNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPCAPETKNNLMA-TEERCGSCGKNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRIQQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKCDRYQYKRKHSKLMN 1873
+GR + C P F N+ +N E TSTCG G ++ICRNWY + + C CD + S S + DR+I ++T W SG + + G + +NIT++L K +EVYYI ++ G LPDS+ +YK G +W PW +FS C ++FKM + + Q N C K + VLPF+ + GR S P S+ + EW+++TD+ ISL+ +K P H +SD+ +G R +C GHA + LI N CDC H T G +C +C + D+PW+ + N+C+ C C NH+++C F +YK + G+HG CL C H+T G C+ C+ +YR+ P+ HPQ C C CH GS+ Q+ C+ G C C+ GV G+ C+RCK GY+Q+ S+ PC P + MA T C C + R RI F+KFC++ + + + SKEIHG R+ +++ +IWR+ +++ + Y W+ + D+RC CP ++LG +YL+LT+ K YL EL +D +S L W + W RRL F+ RE++GKC+ QY +K K+++
Sbjct: 33 NGRPQQCLPSFTNIIENVKAETTSTCGTNGP-EQICRNWYDKTTAEARQYCSICDAK--HRSYPPSHITDRHIPKNQTCWFSGPLQENPGQNEVNITVSLKKSFEVYYIALQICGTLPDSIAIYKSLDFGVSWKPWQFFSQDCYRAFKMPTTNEQNSQITPANIHEVLCVELKAPERYNDYGQAETVLPFSTIYGRPSGPPWSQDLVEWMTMTDLRISLMRFTPEKSTPYRLKELYYHTESLPQAPPNLVNHKTEPIVHFGLSDLAVGGRCKCNGHANRCLIDDNDEIKCDCHHNTEGQECERCKSNYLDRPWQ--RATRQNANVCKLCQCYNHSDKCMFSMGMYKQTRGQHGGVCLECKHNTEGHACDECAAFHYRDITKPVTHPQACTSCKCHTIGSIKQNECDKKTGQCYCRDGVTGQTCDRCKDGYKQSNSTVRPCIPSSGMADMANTSPVCKKCNEKRKRIVFKKFCRRKSVFRATALSKEIHGNMIRYELKIDEIWRVDKNVWEFLPRETPAMHTYRAWINSEDVRCNCPKVELGQSYLILTRAKYYLYLRNKELYIDSRSVMLPWHNSWERRLQKFQKREKRGKCE--QYFKKKMKIID 631
BLAST of Netrin 1 vs. Planmine SMEST
Match: SMESG000050895.1 (SMESG000050895.1) HSP 1 Score: 375.17 bits (962), Expect = 6.372e-121 Identity = 213/606 (35.15%), Postives = 325/606 (53.63%), Query Frame = 2
Query: 146 SGRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGK-CLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSFK--HSVQNITSNIKVLPFNPMTGRTSLLPNSRLIKEWVSVTDIMISLLTDGSKK-------------------------RNKSPYDHLSISDITIGARSQCYGHA-KSLIKKNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPCAPETKNNLMA-TEERCGSCGKNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRIQQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKCDRYQYKRKHSKLMN 1873
+GR + C P F N+ +N E TSTCG G ++ICRNWY + + C CD + S S + DR+I ++T W SG + + G + +NIT++L K +EVYYI ++ G LPDS+ +YK G +W PW +FS C ++FKM + + Q N C K + VLPF+ + GR S P S+ + EW+++TD+ ISL+ +K P H +SD+ +G R +C GHA + LI N CDC H T G +C +C + D+PW+ + N+C+ C CN H+ C F+ +IY ++ +G C++C HHTTG +C +C G++RN P H C C CH GS+ Q+ C+ G C C+ GV G+ C+RCK GY+Q+ S+ PC P + MA T C C + R RI F+KFC++ + + + SKEIHG R+ +++ +IWR+ +++ + Y W+ + D+RC CP ++LG +YL+LT+ K YL EL +D +S L W + W RRL F+ RE++GKC+ QY +K K+++
Sbjct: 33 NGRPQQCLPSFTNIIENVKAETTSTCGTNGP-EQICRNWYDKTTAEARQYCSICDAK--HRSYPPSHITDRHIPKNQTCWFSGPLQENPGQNEVNITVSLKKSFEVYYIALQICGTLPDSIAIYKSLDFGVSWKPWQFFSQDCYRAFKMPTTNEQNSQITPANIHEVLCVELKAPERYNDYGQAETVLPFSTIYGRPSGPPWSQDLVEWMTMTDLRISLMRFTPEKSTPYRLKELYYHTESLPQAPPNLVNHKTEPIVHFGLSDLAVGGRCKCNGHANRCLIDDNDEIKCDCHHNTEGQECERCKSNYLDRPWQ--RATRQNANVCKYCDCNLHSYRCYFNSQIYVRNNYTNGGVCINCRHHTTGNNCENCENGFFRNWTKPQEHRAACTSCKCHTIGSIKQNECDKKTGQCYCRDGVTGQTCDRCKDGYKQSNSTVRPCIPSSGMADMANTSPVCKKCNEKRKRIVFKKFCRRKSVFRATALSKEIHGNMIRYELKIDEIWRVDKNVWEFLPRETPAMHTYRAWINSEDVRCNCPKVELGQSYLILTRAKYYLYLRNKELYIDSRSVMLPWHNSWERRLQKFQKREKRGKCE--QYFKKKMKIID 631
BLAST of Netrin 1 vs. Planmine SMEST
Match: SMESG000076695.1 (SMESG000076695.1) HSP 1 Score: 365.54 bits (937), Expect = 2.346e-117 Identity = 212/627 (33.81%), Postives = 334/627 (53.27%), Query Frame = 2
Query: 149 GRAKFCEPDFINVAQNATFEVTSTCGQKGSTDRICRNWYTSSGLNEGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQLPDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPACFSF--KHSVQNITSNIKVLPFNPMTGRTSLLPNSRLIKEWVSVTDIMISL--LTD------------------GSKKRNKSPYD---------HLSISDITIGARSQCYGHA-KSLIKKNGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPC--------APETKN--------NLMATEERCGSCGKNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRIQQSLKSQVNLYESEYSIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKCDRYQYKRKHSKLMNSR*N 1885
G+ +C P FIN+AQ A VTS+CG+ D IC +W S + C CD+ +I + D Q + T W SG + G++ +NI+I K +EV+Y+ ++F +LPDS+ +YK NGK W+PW Y+S +C+K+F + ++ IQ+ +SP CF K N+ +LPF+ GR N LIK W+++TD+ I+L D G N S + + +SD+++G R QCYGH+ K +I +G VCDC H T G DC +C + +D W+ P C+RC CN H++ CKF++ +Y + K G C C H+T G C+ C GYYR+ P++H C+KC CH GS + C+ G C CK GV G+ C++CK GY QT+S PC + +TK ++ TE C C + R RIRF+KFC++ + I+SKE+HG+ RF +++ ++WR+ + ++ E P+W+ + ++RC CPN+ LG +YL+L K K+ ++ ++ EL++D ++ L W W RRL F+ + + KC+++ K+ SK+ ++R N
Sbjct: 37 GKDYYCTPPFINIAQRAKITVTSSCGEILPDD-ICPHWKYYSSNHFRDCSICDNDRKNSIYSIKSLTDPLNQFNHTCWFSGPVYNQHGLNHVNISIAFKKNFEVFYVALQFCSELPDSITIYKSHDNGKKWIPWQYYSKNCQKAFNLPPTQEFPIQEGLEYSLSPTCFDLNQKQKDGNLGQAESILPFSSTIGRKM---NDTLIK-WITITDLRITLSRFDDQMSSYFDYGKYFLLKHRRGRDILNMSTFSVNDKSMSKVYYGVSDLSVGGRCQCYGHSNKCIINNDGNIVCDCKHNTEGADCDKCKEGFHDLQWERASKLNPFS--CKRCQCNLHSQHCKFEKNVYMKTGQKSGGVCRKCQHNTAGNQCHFCDNGYYRDWTKPLSHDLACKKCKCHAVGSTNVNSCDKKLGQCFCKEGVIGRKCDKCKIGYLQTRSMLKPCVKTYDIIESSQTKKSDLNSKYMDMPNTESECKPCDEKRKRIRFKKFCRRDSVFMGVIESKEVHGKMVRFELQIKEVWRVSGKISQFISEISQEQYAVPLWISHEEVRCDCPNISLGRSYLILMKYKIYHHRHREELLLDSRTVMLQWRPSWERRLQKFQTKANEEKCEKFLQKK--SKIKHNRIN 654
BLAST of Netrin 1 vs. Planmine SMEST
Match: SMESG000006806.1 (SMESG000006806.1) HSP 1 Score: 337.035 bits (863), Expect = 1.842e-106 Identity = 224/652 (34.36%), Postives = 343/652 (52.61%), Query Frame = 2
Query: 128 SSCFSESGRAKFCEPDFINVAQNAT-FEVTSTCGQKGSTDRICRNWYTSSGLNEGKCLKCDDRIPELSRNISLVFDRNIQGHETVWVSGRIPKFSGIHSINITINLNKLYEVYYIIVEFAGQL-PDSMVLYKRDKNGKTWLPWNYFXXXXXXXXXXXXXXPLHIQQRSFNKISPA-------CFSFKHS-VQNITSNIKVLPFNPMTGRTSLLPN-SRLIKEWVSVTDIMISLL----------TDG-------------------------------SKKRNKSPY----DHLSISDITIGARSQCYGHAKSLIKK--NGTTVCDCLHKTAGPDCSQCLKTHNDQPWKIIFPSEPAGNICERCFCNNHAEECKFDEKIYKNSSGKHGSRCLHCLHHTTGPHCNSCSPGYYRNRAFPINHPQVCQKCGCHPYGSLPQSYCNPDNGICQCKIGVEGKLCNRCKKGYQQTKSSSTPCAPETKNNLM----ATEERCGSCGKNRPRIRFRKFCKKHIMIKISIQSKEIHGEWTRFGVRVLKIWRIQQS---------LKSQVNLYESEY--SIYPIWVENADIRCKCPNLQLGHTYLLLTKIKLKNYLNQPELIVDRKSSALDWSDDWIRRLDMFKMREEQGKCDRYQYKRKHSK 1864
S C+SE G+A C P F N+ +N E+TSTCGQ RIC + +N C+K + +I+ + D+++ + T W S + ++NIT + KL+E+YYI + L PDS+++ K NG +W W+YFS C +SF ++ P+ S +KI+ + C S S + N+ ++ VL F T + + S W+S T+I I+L DG KKRN D +++DI+IG R QCYGHA +K + T CDC H TAG DC C + D+ W + + A C+RC CN H+++C+F+EK+Y S+ + G C+ C H+T G +C+ C+ GY+R+ + P++H VC KC CHP GS+ C+ N C CK GV G CNRC+KG+QQTKS TPC + + A E RC C R RIRF+K+C+K ++ +++QS+E HGE RF ++V ++RI S L S + +++ S +P+W++ D++CKCP+LQLG TYL++ K+ YL + EL++D+KS AL W W RR+ F +E QG C++++ KR++ +
Sbjct: 33 SLCYSE-GKAFRCVPPFTNIIENMIPDEITSTCGQ-NKKQRICVD-----HINCHSCMK-------DTHSINYLTDQHLNNNITFWASDFVNHGD---NVNITFSFGKLFEIYYISLVLIPLLEPDSIIISKSIDNGTSWTEWHYFSKDCYRSFGIQ---PMDFNHYSDSKITESTVDQSLTCTSIPDSKLGNLVNSPSVLAFT--TNNPNFFSHASDQYNSWMSATNIRITLRKSETNFLNSHLDGFTYPPHLKQYFNRIYRKNNYSSILPVNRKLKQKKRNSPSVLKISDFFALADISIGGRCQCYGHAGRCLKNPVDNTYHCDCQHNTAGKDCEMCREGFVDKRWSVATVNSAAE--CKRCNCNLHSKKCEFNEKLYIVSNKQSGGVCVGCEHNTDGRYCHQCNKGYHRDWSKPLSHHHVCIKCRCHPIGSIYPDVCDQRNSQCTCKTGVGGLSCNRCQKGFQQTKSPITPCVDVIDLHKIPMAQAPENRCQHCNNKRKRIRFKKYCRKDAVLLVTMQSREQHGEMARFEMKVNYVYRIDTSKFTEMHPDFLASMNGIMSNQFIHSTFPMWIKETDLKCKCPSLQLGMTYLVILKLHAIKYLERSELLLDQKSVALPWQKTWERRMKKFSNKEHQGLCEKWKLKRRYYR 660 The following BLAST results are available for this feature:
BLAST of Netrin 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Netrin 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Netrin 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Netrin 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Netrin 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Netrin 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Netrin 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Netrin 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Netrin 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Netrin 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Netrin 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Netrin 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Netrin 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Netrin 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30000153 ID=SMED30000153|Name=Netrin 1|organism=Schmidtea mediterranea sexual|type=transcript|length=1945bpback to top protein sequence of SMED30000153-orf-1 >SMED30000153-orf-1 ID=SMED30000153-orf-1|Name=SMED30000153-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=608bp MNSTNILLILLNIIWKLTIIKGFSSCFSESGRAKFCEPDFINVAQNATFEback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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