SAM domain-containing protein

Overview
NameSAM domain-containing protein
Smed IDSMED30000140
Length (bp)2101
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SAM domain-containing protein (SMED30000140) t-SNE clustered cells

Violin plots show distribution of expression levels for SAM domain-containing protein (SMED30000140) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SAM domain-containing protein (SMED30000140) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SAM domain-containing protein (SMED30000140) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30000140

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 10

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30000140SMESG000041461.1 SmedASXL_012522SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30000140SMESG000041461.1 SmedASXL_012522SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30000140SMESG000041461.1 dd_Smed_v4_19710_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
reproductive organSMED30000140SMESG000041461.1 Contig32731newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30000140SMESG000041461.1 Contig32731uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30000140SMESG000041461.1 Contig27143newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30000140SMESG000041461.1 Contig27143uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
zeta neoblastSMED30000140SMESG000041461.1 dd_Smed_v4_19710_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
neoblastSMED30000140SMESG000041461.1 SmWIOct06_011737GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30000140SMESG000041461.1 SMED_20461_V2_1GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SAM domain-containing protein vs. Ensembl Human
Match: SAMD15 (sterile alpha motif domain containing 15 [Source:HGNC Symbol;Acc:HGNC:18631])

HSP 1 Score: 55.4546 bits (132), Expect = 2.943e-7
Identity = 26/69 (37.68%), Postives = 41/69 (59.42%), Query Frame = 3 
Query:  525 SLIQLCNFQNAIYANRIDGKRLIFMEASNLVKMGIKDFDHIKAITAAVRERLNIEDPDYGRRIYLPPRN 731
            S +    ++     N I G++LI +  SNL +MGI +F+ +KAI+   +E L IE+P + R I LP R+
Sbjct:  555 SQLGFPQYKECFITNFISGRKLIHVNCSNLPQMGITNFEDMKAISRHTQELLEIEEPLFKRSISLPYRD 623
[Close]
BLAST of SAM domain-containing protein vs. Ensembl Xenopus
Match: smg8 (SMG8 nonsense mediated mRNA decay factor [Source:Xenbase;Acc:XB-GENE-965595])

HSP 1 Score: 54.299 bits (129), Expect = 3.125e-8
Identity = 23/64 (35.94%), Postives = 39/64 (60.94%), Query Frame = 3 
Query:  546 FQNAIYANRIDGKRLIFMEASNLVKMGIKDFDHIKAITAAVRERLNIEDPDYGRRIYLPPRNKM 737
            ++     N I G++LI +  S L ++G+ DF H+K I+  +R+ LNI +P + R + LP R+ M
Sbjct:   41 YEACFTQNYISGRKLIHVNCSTLPQIGVTDFQHMKEISRLIRDLLNITEPLWTRSVSLPHRDNM 104
[Close]
BLAST of SAM domain-containing protein vs. Ensembl Mouse
Match: Samd15 (sterile alpha motif domain containing 15 [Source:MGI Symbol;Acc:MGI:2685109])

HSP 1 Score: 56.225 bits (134), Expect = 1.187e-7
Identity = 27/69 (39.13%), Postives = 41/69 (59.42%), Query Frame = 3 
Query:  525 SLIQLCNFQNAIYANRIDGKRLIFMEASNLVKMGIKDFDHIKAITAAVRERLNIEDPDYGRRIYLPPRN 731
            S +    ++     N I+G++LI +  SNL +MGI DF+ +KAI+   R  L IE+P + R I LP R+
Sbjct:  490 SDLGFPQYKECFTENFINGQKLIHVNCSNLPQMGITDFEDMKAISYHTRVLLGIEEPLFSRSISLPYRD 558
[Close]
BLAST of SAM domain-containing protein vs. UniProt/SwissProt
Match: sp|Q95JY5|SAM15_MACFA (Sterile alpha motif domain-containing protein 15 OS=Macaca fascicularis OX=9541 GN=SAMD15 PE=2 SV=2)

HSP 1 Score: 57.3806 bits (137), Expect = 3.349e-7
Identity = 27/69 (39.13%), Postives = 41/69 (59.42%), Query Frame = 3 
Query:  525 SLIQLCNFQNAIYANRIDGKRLIFMEASNLVKMGIKDFDHIKAITAAVRERLNIEDPDYGRRIYLPPRN 731
            S +    ++     N I G++LI +  SNL +MGI +F+ +KAI+   RE L IE+P + R I LP R+
Sbjct:  548 SQLGFPQYKGCFITNFISGRKLIHVNCSNLPQMGITNFEDMKAISRHTRELLEIEEPLFKRSISLPHRD 616
[Close]
BLAST of SAM domain-containing protein vs. TrEMBL
Match: K1RET5 (SAM domain-containing protein OS=Crassostrea gigas OX=29159 GN=CGI_10017842 PE=4 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 6.779e-13
Identity = 36/65 (55.38%), Postives = 48/65 (73.85%), Query Frame = 3 
Query:  543 NFQNAIYANRIDGKRLIFMEASNLVKMGIKDFDHIKAITAAVRERLNIEDPDYGRRIYLPPRNKM 737
            N+++ I +NRIDG++LI +EAS L  +GI DF HIK I+ AVRE L IE+PD+ R I LPPR  +
Sbjct:   38 NYKSCITSNRIDGRKLITLEASQLPNIGITDFKHIKTISKAVRELLFIEEPDWKRSISLPPRENL 102
[Close]
BLAST of SAM domain-containing protein vs. TrEMBL
Match: A0A267ECP4 (SAM domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig029633g1 PE=4 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 2.836e-12
Identity = 31/71 (43.66%), Postives = 51/71 (71.83%), Query Frame = 3 
Query:  525 SLIQLCNFQNAIYANRIDGKRLIFMEASNLVKMGIKDFDHIKAITAAVRERLNIEDPDYGRRIYLPPRNKM 737
            S + L  +++    N I+G+RL+ ++ASNL K+G+ +F H++A++ AVR+ L IE P + RRIYLPPR+ +
Sbjct:   37 SSLGLGQYRDCFLTNGINGRRLVLVDASNLPKIGVHEFQHVQALSGAVRDLLKIESPRWDRRIYLPPRDNL 107
[Close]
BLAST of SAM domain-containing protein vs. TrEMBL
Match: A0A267DFE1 (SAM domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig031519g6 PE=4 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 3.523e-12
Identity = 31/71 (43.66%), Postives = 51/71 (71.83%), Query Frame = 3 
Query:  525 SLIQLCNFQNAIYANRIDGKRLIFMEASNLVKMGIKDFDHIKAITAAVRERLNIEDPDYGRRIYLPPRNKM 737
            S + L  +++    N I+G+RL+ ++ASNL K+G+ +F H++A++ AVR+ L IE P + RRIYLPPR+ +
Sbjct:   37 SSLGLGQYRDCFLTNGINGRRLVLVDASNLPKIGVHEFQHVQALSGAVRDLLKIESPRWDRRIYLPPRDNL 107
[Close]
BLAST of SAM domain-containing protein vs. TrEMBL
Match: A0A267DH85 (SAM domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig029199g1 PE=4 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 3.733e-12
Identity = 31/71 (43.66%), Postives = 51/71 (71.83%), Query Frame = 3 
Query:  525 SLIQLCNFQNAIYANRIDGKRLIFMEASNLVKMGIKDFDHIKAITAAVRERLNIEDPDYGRRIYLPPRNKM 737
            S + L  +++    N I+G+RL+ ++ASNL K+G+ +F H++A++ AVR+ L IE P + RRIYLPPR+ +
Sbjct:   37 SSLGLGQYRDCFLTNGINGRRLVLVDASNLPKIGVHEFQHVQALSGAVRDLLKIESPRWDRRIYLPPRDNL 107
[Close]
BLAST of SAM domain-containing protein vs. TrEMBL
Match: A0A1I8FG85 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 7.566e-11
Identity = 31/71 (43.66%), Postives = 51/71 (71.83%), Query Frame = 3 
Query:  525 SLIQLCNFQNAIYANRIDGKRLIFMEASNLVKMGIKDFDHIKAITAAVRERLNIEDPDYGRRIYLPPRNKM 737
            S + L  +++    N I+G+RL+ ++ASNL K+G+ +F H++A++ AVR+ L IE P + RRIYLPPR+ +
Sbjct:   75 SSLGLGQYRDCFLTNGINGRRLVLVDASNLPKIGVHEFQHVQALSGAVRDLLKIESPRWDRRIYLPPRDNL 145
[Close]
BLAST of SAM domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000040150.1 (pep primary_assembly:Astyanax_mexicanus-2.0:3:46330990:46335498:-1 gene:ENSAMXG00000031813.1 transcript:ENSAMXT00000040150.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 57.3806 bits (137), Expect = 7.897e-10
Identity = 26/64 (40.62%), Postives = 39/64 (60.94%), Query Frame = 3 
Query:  546 FQNAIYANRIDGKRLIFMEASNLVKMGIKDFDHIKAITAAVRERLNIEDPDYGRRIYLPPRNKM 737
             Q     N I G++LI++    L ++G+ DF H+KAI+A VRE L + +P + R I  PPR+ M
Sbjct:   19 LQACFTENLITGRKLIYVNCRYLPRLGMTDFQHMKAISAHVRELLGVSEPLWSRSIADPPRDNM 82
[Close]
BLAST of SAM domain-containing protein vs. Ensembl Nematostella
Match: EDO37353 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SG04])

HSP 1 Score: 63.1586 bits (152), Expect = 4.918e-12
Identity = 35/85 (41.18%), Postives = 49/85 (57.65%), Query Frame = 3 
Query:  567 NRIDGKRLIFMEASNLVKMGIKDFDHIKAITAAVRERLNIEDPDYGRRIYLPPRNKMDSSANKEN-VGAPKDTAFILIYHRNAPK 818
            N ++G+RLI ++AS L +MG+ DF+HIK I+  +RE L IEDP + R I LP R  +     K++  GA  D      Y R   K
Sbjct:   38 NLVNGRRLIQVDASTLPRMGVTDFEHIKFISKKIREALGIEDPYWNRSISLPHREVLGMYLEKKSKTGAEADILTFEEYIRQLEK 122
[Close]
BLAST of SAM domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000045480.1 (sterile alpha motif domain containing 15 [Source:NCBI gene;Acc:101158569])

HSP 1 Score: 55.0694 bits (131), Expect = 9.093e-9
Identity = 26/57 (45.61%), Postives = 38/57 (66.67%), Query Frame = 3 
Query:  567 NRIDGKRLIFMEASNLVKMGIKDFDHIKAITAAVRERLNIEDPDYGRRIYLPPRNKM 737
            N+I+G++LIF+  SNL K+GI DF  ++ I+A VRE L I +  +   I  PPR+ M
Sbjct:   44 NQINGRKLIFVNCSNLPKLGIVDFKDMQVISARVRELLGITETPWSHSIADPPRDAM 100
[Close]
BLAST of SAM domain-containing protein vs. Planmine SMEST
Match: SMESG000028623.1 (SMESG000028623.1)

HSP 1 Score: 238.039 bits (606), Expect = 2.059e-67
Identity = 141/220 (64.09%), Postives = 154/220 (70.00%), Query Frame = 3 
Query:  972 QRVLIAEQSAALRRIFTDAELRARVPGCIWAQSLNIIPAQHLPTEPAQHTRSEPTPSQIVEPPQSTMAEPRPGATQXXXXXXX--------------XXXXXXXXXXXXDLPPPYHSSNSST*IP-ILSTHPL**ISPPRSIYIDLVGDASEAPSLNHALDRLGLDMGDISVLTGSVPMSPTPANEPGPYTSVELNEITSPPLHRQPKR*SLPTDIDAED 1586
            QRVLIAEQSA LRRIFTD ELRARVPGCIWAQSLNIIPAQHLP   AQ   SEP P  IV+P QST A+PRPG T+                      +ES+R   RE+D PPPY         P I+    L  +SP RSIYIDLVGD +EAPSLNHALDRLGLD+G+I VLT SVPMSP PANEPG  T VELNE+TSPPLH+QPKR  LPTDIDAED
Sbjct:  631 QRVLIAEQSADLRRIFTDVELRARVPGCIWAQSLNIIPAQHLPVGSAQQPPSEPAPPLIVKPLQSTTAKPRPGVTRRRPNRNRRRRERRRAEANLLRATESTRMSNREDDPPPPYQEFEFPDFDPDIIYAPSLINLSPLRSIYIDLVGDTTEAPSLNHALDRLGLDVGEILVLTVSVPMSPPPANEPGLSTRVELNEMTSPPLHQQPKRRCLPTDIDAED 850
[Close]
BLAST of SAM domain-containing protein vs. Planmine SMEST
Match: SMESG000041408.1 (SMESG000041408.1)

HSP 1 Score: 168.703 bits (426), Expect = 1.339e-64
Identity = 109/243 (44.86%), Postives = 145/243 (59.67%), Query Frame = 3 
Query:  903 EEVISKITRLSRARDELVVESRSQRVLIAEQSAALRRIFTDAELRARVPGCIWAQSLNIIPAQHLPTEPAQHTRSEPTPSQIVEPPQSTMAEPRPGAT--------------QXXXXXXXXXXXXXXXXXXXDLPPPYHSSNSST*IPILSTHPL**I-SPPRSIYIDLVGDASEAPSLNHALDRLGLDMGDISVLTGSVPMSPTPANEPGPYTSVELNEITSPPLHRQPKR*SLPTDIDAED 1586
            +++  KI RL+RARDEL++ESRSQR+LIAEQS ALRRIFT+AELRAR+PGC  AQSL+IIPAQHLP        S PTPS   EP Q+T  +P PG +              + E+      E+          PP Y         P +   P+  I  PP S+YIDL+ D +E P+++ AL+RL L+M +IS+L+G VP S T  + PG  T +E N I +  L+R  KR  LP DID+++
Sbjct:   52 KKLFQKIARLTRARDELIIESRSQRILIAEQSTALRRIFTEAELRARLPGCSLAQSLDIIPAQHLP--------SNPTPSLPNEPSQNTTVDPTPGVSRRRPNQNRRRRERRRAEASLLRAIETVGTTSGISGPPPAYQEFEFPEFDPDIIYAPMVVIPPPPPSVYIDLIEDITETPTVDQALERLCLNMEEISILSGHVPTSRTSTSAPGTSTQIESNPIDNSSLYRHSKRRCLPVDIDSDN 286

HSP 2 Score: 98.2117 bits (243), Expect = 1.339e-64
Identity = 45/57 (78.95%), Postives = 52/57 (91.23%), Query Frame = 1 
Query:  751 IKKMSELPRIPRLSSSIIATRQRLEQLEKDHSILQVKYQKLKGTWT*PESTRKLFQK 921
            +  M+ELPRIPRLS SI+ATRQRLEQLEKDH++LQ KYQKLKGTW+ PEST+KLFQK
Sbjct:    1 MTNMTELPRIPRLSPSIVATRQRLEQLEKDHTVLQAKYQKLKGTWSSPESTKKLFQK 57
[Close]
BLAST of SAM domain-containing protein vs. Planmine SMEST
Match: SMESG000028450.1 (SMESG000028450.1)

HSP 1 Score: 180.259 bits (456), Expect = 1.152e-63
Identity = 114/242 (47.11%), Postives = 143/242 (59.09%), Query Frame = 3 
Query:  903 EEVISKITRLSRARDELVVESRSQRVLIAEQSAALRRIFTDAELRARVPGCIWAQSLNIIPAQHLPTEPAQHTRSEPTPSQIVEPPQSTMAEPRPGATQXXXXXXXXXXXXXXXXXXX--------------DLPPPYHSSNSST*IPILSTHPL**ISPPRSIYIDLVGDASEAPSLNHALDRLGLDMGDISVLTGSVPMSPTPANEPGPYTSVELNEITSPPLHRQPKR*SLPTDIDAED 1586
            +++  KI+RLSRARDEL+VESRSQRVLI EQ   LR+IFTDAELRAR+PG IWA++LNIIPAQHLP +      S P  S   E PQ+ + EPR GA +       R    RR +                 DLPPPYH          ++ H     SP RSI+I+LV D +E P  N  L RLGLD  +I++LTG+VP      +EPGP T V+ N   +PP +R  KR   PTDIDA++
Sbjct:  204 KKLYQKISRLSRARDELIVESRSQRVLIKEQGTTLRQIFTDAELRARIPGSIWARNLNIIPAQHLPLDVPLPVHSVPA-SAGTEHPQTPVVEPRTGAARRRPNRNCRRHERRRRETHLLSSTEPISAPYSIHDLPPPYH----------VTFH-----SPARSIHINLVDDENEVPVANQTLGRLGLDPSEIAILTGNVPGCLPHPDEPGPSTCVDFNINVNPPPYRPAKRRCFPTDIDADE 429

HSP 2 Score: 83.9593 bits (206), Expect = 1.152e-63
Identity = 39/53 (73.58%), Postives = 46/53 (86.79%), Query Frame = 1 
Query:  763 SELPRIPRLSSSIIATRQRLEQLEKDHSILQVKYQKLKGTWT*PESTRKLFQK 921
            + LPRIPRLS SIIATRQRLEQLEKDH+ LQ KYQK+KGTW  P++ +KL+QK
Sbjct:  157 NNLPRIPRLSPSIIATRQRLEQLEKDHAALQDKYQKVKGTWLSPDAAKKLYQK 209
[Close]
BLAST of SAM domain-containing protein vs. Planmine SMEST
Match: SMESG000070051.1 (SMESG000070051.1)

HSP 1 Score: 198.364 bits (503), Expect = 8.027e-58
Identity = 123/254 (48.43%), Postives = 154/254 (60.63%), Query Frame = 3 
Query:  849 LASQISEIKRNVDLTRIDEEVISKITRLSRARDELVVESRSQRVLIAEQSAALRRIFTDAELRARVPGCIWAQSLNIIPAQHLPTEPAQHTRSEPTPSQIVEPPQSTMAEPRPGATQXXXXXXXXXX-------XXXXXXXXXDLPPPYHSSNSST*IPILSTHPL**I-SPPRSIYIDLVGDASEAPSLNHALDRLGLDMGDISVLTGSVPMSPTPANEPGPYTSVELNEITSPPLHRQPKR*SLPTDIDAED 1586
            L S+  ++K         +++  KI+RLSRARD+LVVESRSQR+LIAEQSAALRRIFT+AELRAR+PGC WAQ LNIIPAQHL         S PTP    EPPQ+ + EPRPG ++                 ++    R  D PP Y         P +   P   I +PP SIYIDLV DA + P+L++AL +LGLD+G+I++LTG VP SPT    PG  T +  N   + PL+ QPK    PTDIDAED
Sbjct:   31 LQSKYEKLKGTWSSPEFTQKLFQKISRLSRARDDLVVESRSQRILIAEQSAALRRIFTEAELRARLPGCSWAQGLNIIPAQHL--------LSNPTPPN--EPPQNVVIEPRPGVSRRRRKRRREEANLLRAIETTGTTSRANDPPPAYQEFEFPDFDPNIIYAPAVTIHTPPPSIYIDLVEDAIKVPTLDNALGQLGLDIGEITILTGRVPTSPTLVFAPGTSTQMGENSAVNSPLYHQPKHRRFPTDIDAED 274

HSP 2 Score: 82.4185 bits (202), Expect = 6.695e-17
Identity = 40/54 (74.07%), Postives = 48/54 (88.89%), Query Frame = 1 
Query:  760 MSELPRIPRLSSSIIATRQRLEQLEKDHSILQVKYQKLKGTWT*PESTRKLFQK 921
            MS+L  IPRLS SIIATRQRL+QLE+DH++LQ KY+KLKGTW+ PE T+KLFQK
Sbjct:    1 MSDLHWIPRLSPSIIATRQRLKQLERDHAVLQSKYEKLKGTWSSPEFTQKLFQK 54
[Close]
BLAST of SAM domain-containing protein vs. Planmine SMEST
Match: SMESG000059849.1 (SMESG000059849.1)

HSP 1 Score: 166.392 bits (420), Expect = 1.336e-57
Identity = 107/248 (43.15%), Postives = 145/248 (58.47%), Query Frame = 3 
Query:  903 EEVISKITRLSRARDELVVESRSQRVLIAEQSAALRRIFTDAELRARVPGCIWAQSLNIIPAQHLPTEPAQHTRSEPTPSQIVEPPQSTMAEPRPG--------------ATQXXXXXXXXXXXXXXXXXXXDLPPPYHSSN------SST*IPILSTHPL**ISPPRSIYIDLVGDASEAPSLNHALDRLGLDMGDISVLTGSVPMSPTPANEPGPYTSVELNEITSPPLHRQPKR*SLPTDIDAED 1586
            +++  KI+RLSR RD+L++ESR QR+LI EQ   LR+IFTDAELRAR+PG + A++LNIIPAQHL  E    T S PTP  I E  Q+ +AEPR G                +  +   S +E S  P   +DLPPPY               PI+  H     SP RSI+IDLV +  E P++N +L+RL LD  +I+VLTG +P      +EPGP + ++ N   +PP +R  KR   PTD+DA++
Sbjct:  537 KKLYQKISRLSRVRDDLIIESRGQRILIKEQGTTLRQIFTDAELRARIPGSVLARNLNIIPAQHLSLETPPVTLSTPTPV-IAESSQTPVAEPRSGAARRRLNRNRRRREQRRRAASLFSATEPSNAPYSIQDLPPPYQEFEFPDMEADIIYAPIVVLH-----SPARSIHIDLVDEVDETPTVNRSLERLMLDPDEIAVLTGRLPERLPLPDEPGPSSRIDFNIHINPPPYRPAKRRCFPTDMDADE 778

HSP 2 Score: 77.411 bits (189), Expect = 1.336e-57
Identity = 36/53 (67.92%), Postives = 45/53 (84.91%), Query Frame = 1 
Query:  763 SELPRIPRLSSSIIATRQRLEQLEKDHSILQVKYQKLKGTWT*PESTRKLFQK 921
            + LPRIPRLS SIIATRQRLEQLE++H+  Q KYQK+KGTW  P++ +KL+QK
Sbjct:  490 TNLPRIPRLSPSIIATRQRLEQLEEEHAAHQKKYQKVKGTWLSPDAAKKLYQK 542
The following BLAST results are available for this feature:
BLAST of SAM domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
SAMD152.943e-737.68sterile alpha motif domain containing 15 [Source:H... [more]
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BLAST of SAM domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SAM domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SAM domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SAM domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
smg83.125e-835.94SMG8 nonsense mediated mRNA decay factor [Source:X... [more]
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BLAST of SAM domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Samd151.187e-739.13sterile alpha motif domain containing 15 [Source:M... [more]
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BLAST of SAM domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1
Match NameE-valueIdentityDescription
sp|Q95JY5|SAM15_MACFA3.349e-739.13Sterile alpha motif domain-containing protein 15 O... [more]
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BLAST of SAM domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
K1RET56.779e-1355.38SAM domain-containing protein OS=Crassostrea gigas... [more]
A0A267ECP42.836e-1243.66SAM domain-containing protein (Fragment) OS=Macros... [more]
A0A267DFE13.523e-1243.66SAM domain-containing protein (Fragment) OS=Macros... [more]
A0A267DH853.733e-1243.66SAM domain-containing protein (Fragment) OS=Macros... [more]
A0A1I8FG857.566e-1143.66Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of SAM domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSAMXT00000040150.17.897e-1040.63pep primary_assembly:Astyanax_mexicanus-2.0:3:4633... [more]
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BLAST of SAM domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SAM domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SAM domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO373534.918e-1241.18Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SAM domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSORLT00000045480.19.093e-945.61sterile alpha motif domain containing 15 [Source:N... [more]
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BLAST of SAM domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000028623.12.059e-6764.09SMESG000028623.1[more]
SMESG000041408.11.339e-6444.86SMESG000041408.1[more]
SMESG000028450.11.152e-6347.11SMESG000028450.1[more]
SMESG000070051.18.027e-5848.43SMESG000070051.1[more]
SMESG000059849.11.336e-5743.15SMESG000059849.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30000140 ID=SMED30000140|Name=SAM domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=2101bp
AATTGAAACTTTGTTTCAATAATAAAATGGATGATAAATTAATTGTTTTA
GGCGAAAGCGTTTGTGAAGAAGTAAATGAAAACTGGAACATAAATCGGGA
TGACGAAGATGATTTAAGAGTTCCAGTTGCTTTGTTTTGGTCTGTTGAAG
ACGCTGCAAATTTTATGAAAGATATTGGATTCCCTCAATATATGGTTTTT
AATTTTATTAGAATTTGGTCTATATTTCAGCATTTTATTAATATAATCAA
TAAACTTAATGTTTCGTTGGTGTTACTAAATAATTTTCTTTATGAACGCC
TTACTCAATTTGTGTATAATATCCAAGTAATTACTTTATGCGTCAAAATA
ATTTGATGTTTCTAAATTTTCTGCAATCAACTGGGTGAAACAATTAGTGG
ATATCTCTGATTTTTAATGGGTTTCATTTGAAATTATTGTACTTAAAATT
TATAATGCATTTTTTATTCGTAAAAGAATTATAATATGTAGCTCGTTATT
TTTATTAATAAAATTCTAAGTTTCAGTTTAATTCAACTATGTAATTTTCA
GAACGCCATTTATGCAAATAGGATAGATGGTAAACGGTTAATATTCATGG
AAGCATCTAATCTAGTAAAAATGGGAATTAAAGATTTTGATCATATTAAA
GCAATTACGGCTGCTGTAAGAGAGCGCTTAAATATCGAAGATCCTGATTA
TGGAAGAAGAATTTATCTTCCTCCACGAAACAAAATGGATTCCTCAGCTA
ATAAAGAAAATGTCGGAGCTCCCAAGGATACCGCGTTTATCCTCATCTAT
CATCGCAACGCGCCAAAGACTCGAACAACTGGAGAAAGACCATTCGATCT
TGCAAGTCAAATATCAGAAATTAAAAGGAACGTGGACCTAACCCGAATCG
ACGAGGAAGTTATTTCAAAAATCACCCGCCTGTCTCGGGCCAGAGATGAA
TTGGTGGTCGAATCTCGTAGCCAACGAGTGCTAATAGCAGAACAGAGCGC
CGCCCTCAGGCGAATATTCACAGACGCCGAACTCCGAGCTCGTGTACCTG
GCTGCATCTGGGCTCAAAGCCTAAACATTATACCAGCTCAACACCTCCCT
ACAGAACCAGCTCAGCATACTCGTTCAGAGCCAACCCCATCTCAAATTGT
CGAACCCCCTCAGAGCACAATGGCCGAACCTAGGCCCGGAGCGACCCAAC
CGGAATCGCCGTCGCCGAGCAGAAGCGAATCTTCTAGGAGGCCCAAACGA
GAGGAGGATCTACCCCCACCGTATCATAGTTCGAATTCCTCGACTTAGAT
CCCGATATTATCTACGCACCCTCTCTAATAAATCTCTCCTCCACGATCTA
TCTATATAGACTTGGTGGGAGATGCATCAGAAGCTCCATCCCTAAACCAC
GCTCTAGACCGACTAGGCCTCGATATGGGCGACATTTCGGTTCTTACCGG
AAGCGTTCCAATGAGCCCTACTCCGGCTAACGAACCAGGACCCTACACCA
GCGTGGAGCTCAACGAAATAACCAGCCCTCCTCTCCATCGACAGCCAAAA
CGCTGATCTCTGCCGACAGACATCGATGCAGAGGATTAGAATTGAGGCTG
TCGAGGTCTGCCGCGATAACCGCTCTGGTTTTCAAAATTGCCATCCTCGT
CAGAGCGACGAAGCTATTTAATCTTTCGTAACATTTCCCAAACTCTTATC
TCGAGCAATGGGCGCGTATGATACATGATAACTGCATGATATTTTATAAT
CTGTTCTTGTCTCTTGCCTATTATTTTTAAATGGTATATGTAAAAATAAA
ATAAATTGTGTTAACAATCTTTGTAGTGTTTGTTTTTCGGTAAAGTGAAC
GTGAATGATTAGACCAAAGGAGTAAAATAATAATGACGAACTAACAATTA
ATTGAACGGAGAGACTAAAGGTACATCTGCCAATAGAGAAAGCTGTTAAT
TAACTAACAATACTAAACCGGAGTAAAACCCTTATTGAAATTCTCACAAT
TCCAGGAATAGGGTAAGAGTGGACTCTAAGGGTTCTGTGATAGTACAACT
TCAAAGTAAAGTGCGTAAGTCCTTCTATCGGCAGCCAATGATCGGGAAAA
C
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protein sequence of SMED30000140-orf-1

>SMED30000140-orf-1 ID=SMED30000140-orf-1|Name=SMED30000140-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=234bp
MEASNLVKMGIKDFDHIKAITAAVRERLNIEDPDYGRRIYLPPRNKMDSS
ANKENVGAPKDTAFILIYHRNAPKTRTTGERPFDLASQISEIKRNVDLTR
IDEEVISKITRLSRARDELVVESRSQRVLIAEQSAALRRIFTDAELRARV
PGCIWAQSLNIIPAQHLPTEPAQHTRSEPTPSQIVEPPQSTMAEPRPGAT
QPESPSPSRSESSRRPKREEDLPPPYHSSNSST*
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protein sequence of SMED30000140-orf-2

>SMED30000140-orf-2 ID=SMED30000140-orf-2|Name=SMED30000140-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=118bp
LKLCFNNKMDDKLIVLGESVCEEVNENWNINRDDEDDLRVPVALFWSVED
AANFMKDIGFPQYMVFNFIRIWSIFQHFINIINKLNVSLVLLNNFLYERL
TQFVYNIQVITLCVKII*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0000429neoblast
PLANA:0002089reproductive organ
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR013761SAM/pointed_sf
Vocabulary: biological process
TermDefinition
GO:0000027ribosomal large subunit assembly
GO:0006364rRNA processing
Vocabulary: molecular function
TermDefinition
GO:0003674molecular_function
Vocabulary: cellular component
TermDefinition
GO:0030687preribosome, large subunit precursor
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..43