60S ribosomal protein L4

Overview
Name60S ribosomal protein L4
Smed IDSMED30000134
Length (bp)1238
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of 60S ribosomal protein L4 (SMED30000134) t-SNE clustered cells

Violin plots show distribution of expression levels for 60S ribosomal protein L4 (SMED30000134) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of 60S ribosomal protein L4 (SMED30000134) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for 60S ribosomal protein L4 (SMED30000134) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30000134

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30000134SMESG000009479.1 SmedASXL_009666SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
gutSMED30000134SMESG000009479.1 dd_Smed_v4_211_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
neoblastSMED30000134SMESG000009479.1 dd_Smed_v4_211_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30000134SMESG000009479.1 dd_Smed_v4_211_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
epidermal cellSMED30000134SMESG000009479.1 dd_Smed_v4_211_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
progenitor cellSMED30000134SMESG000009479.1 dd_Smed_v6_211_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30000134SMESG000009479.1 dd_Smed_v6_211_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of 60S ribosomal protein L4 vs. Ensembl Human
Match: RPL4 (ribosomal protein L4 [Source:HGNC Symbol;Acc:HGNC:10353])

HSP 1 Score: 337.421 bits (864), Expect = 2.308e-112
Identity = 196/343 (57.14%), Postives = 249/343 (72.59%), Query Frame = 2
Query:  110 AMSRPLINVYKVNLPTDDSKPKLECIIDGSVKFPSVFNAPLRMDILSSVHDLIRKNSRQPYAVSTGAGHNNSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGHMFAPTKVWRKWHHRVPIQQRRYAMVTAIAATGYTPLVAARGHKVEKVPELPLVISEEYEGLKKTKMAVKVLKGCGVWPDIEKVKDSRKVRAGRGKMRNRRYVQKCGPIVIYKSDNGITKAFRNIPGVTXXXXXXXXXXXXSPGGHMGRLTIWTKPAIEELEKIYGSWKTASQSKVDFLLPAPTMKNADLMRIINSEEIQSVLKARNMXXXXXXXXXXXXXXXNDGVMAKLNPYA 1138
            A +RPLI+VY     +             +V  P+VF AP+R DI++ VH  +RKN+RQPYAVS  AGH  SAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGG MFAPTK WR+WH RV   Q+RYA+ +A+AA+    LV ++GH++E+VPELPLV+ ++ EG KKTK AV +LK    W DI+KV  S+++RAG+GKMRNRR +Q+ GP +IY  DNGI KAFRNIPG+TL+NV  LNILKL+PGGH+GR  IWT+ A  +L+++YG+W+ A+  K ++ LP   M N DL RI+ S EIQ  L+A   + +     +KKNPLKN  +M KLNPYA
Sbjct:    2 ACARPLISVYSEKGESSGK----------NVTLPAVFKAPIRPDIVNFVHTNLRKNNRQPYAVSELAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKTWRRWHRRVNTTQKRYAICSALAASALPALVMSKGHRIEEVPELPLVVEDKVEGYKKTKEAVLLLKKLKAWNDIKKVYASQRMRAGKGKMRNRRRIQRRGPCIIYNEDNGIIKAFRNIPGITLLNVSKLNILKLAPGGHVGRFCIWTESAFRKLDELYGTWRKAASLKSNYNLPMHKMINTDLSRILKSPEIQRALRA--PRKKIHRRVLKKNPLKNLRIMLKLNPYA 332          
BLAST of 60S ribosomal protein L4 vs. Ensembl Human
Match: RPL4 (ribosomal protein L4 [Source:HGNC Symbol;Acc:HGNC:10353])

HSP 1 Score: 218.394 bits (555), Expect = 2.745e-67
Identity = 133/240 (55.42%), Postives = 176/240 (73.33%), Query Frame = 2
Query:  419 MCRGGHMFAPTKVWRKWHHRVPIQQRRYAMVTAIAATGYTPLVAARGHKVEKVPELPLVISEEYEGLKKTKMAVKVLKGCGVWPDIEKVKDSRKVRAGRGKMRNRRYVQKCGPIVIYKSDNGITKAFRNIPGVTXXXXXXXXXXXXSPGGHMGRLTIWTKPAIEELEKIYGSWKTASQSKVDFLLPAPTMKNADLMRIINSEEIQSVLKARNMXXXXXXXXXXXXXXXNDGVMAKLNPYA 1138
            MCRGG MFAPTK WR+WH RV   Q+RYA+ +A+AA+    LV ++GH++E+VPELPLV+ ++ EG KKTK AV +LK    W DI+KV  S+++RAG+GKMRNRR +Q+ GP +IY  DNGI KAFRNIPG+TL+NV  LNILKL+PGGH+GR  IWT+ A  +L+++YG+W+ A+  K ++ LP   M N DL RI+ S EIQ  L+A   + +     +KKNPLKN  +M KLNPYA
Sbjct:    1 MCRGGRMFAPTKTWRRWHRRVNTTQKRYAICSALAASALPALVMSKGHRIEEVPELPLVVEDKVEGYKKTKEAVLLLKKLKAWNDIKKVYASQRMRAGKGKMRNRRRIQRRGPCIIYNEDNGIIKAFRNIPGITLLNVSKLNILKLAPGGHVGRFCIWTESAFRKLDELYGTWRKAASLKSNYNLPMHKMINTDLSRILKSPEIQRALRA--PRKKIHRRVLKKNPLKNLRIMLKLNPYA 238          
BLAST of 60S ribosomal protein L4 vs. Ensembl Human
Match: RPL4 (ribosomal protein L4 [Source:HGNC Symbol;Acc:HGNC:10353])

HSP 1 Score: 189.504 bits (480), Expect = 2.976e-58
Identity = 106/192 (55.21%), Postives = 129/192 (67.19%), Query Frame = 2
Query:  110 AMSRPLINVYKVNLPTDDSKPKLECIIDGSVKFPSVFNAPLRMDILSSVHDLIRKNSRQPYAVSTGAGHNNSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGHMFAPTKVWRKWHHRVPIQQRRYAMVTAIAATGYTPLVAARGHKVEKVPELPLVISEEYEGLKKTKMAVKVLKGCGVWPDIEKV 685
            A +RPLI+VY       +S  K       +V  P+VF AP+R DI++ VH  +RKN+RQPYAVS  AGH  SAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGG MFAPTK WR+WH RV   Q+RYA+ +A+AA+    LV ++             + ++ EG KKTK AV +LK    W DI+KV
Sbjct:    2 ACARPLISVYS---EKGESSGK-------NVTLPAVFKAPIRPDIVNFVHTNLRKNNRQPYAVSELAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKTWRRWHRRVNTTQKRYAICSALAASALPALVMSK-------------VEDKVEGYKKTKEAVLLLKKLKAWNDIKKV 170          
BLAST of 60S ribosomal protein L4 vs. Ensembl Human
Match: RPL4 (ribosomal protein L4 [Source:HGNC Symbol;Acc:HGNC:10353])

HSP 1 Score: 162.925 bits (411), Expect = 5.807e-48
Identity = 91/157 (57.96%), Postives = 107/157 (68.15%), Query Frame = 2
Query:  110 AMSRPLINVYKVNLPTDDSKPKLECIIDGSVKFPSVFNAPLRMDILSSVHDLIRKNSRQPYAVSTGAGHNNSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGHMFAPTKVWRKWHHRV-PIQQRRYAM-VTAIAAT--GYTPLVAARGHKV 568
            A +RPLI+VY       +S  K       +V  P+VF AP+R DI++ VH  +RKN+RQPYAVS  AGH  SAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGG MFAPTK WR+WH R  P++  R  + +   A T    T L  AR HK+
Sbjct:    2 ACARPLISVYS---EKGESSGK-------NVTLPAVFKAPIRPDIVNFVHTNLRKNNRQPYAVSELAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKTWRRWHRRKNPLKNLRIMLKLNPYAKTMRRNTILRQARNHKL 148          
BLAST of 60S ribosomal protein L4 vs. Ensembl Celegans
Match: rpl-4 (60S ribosomal protein L4 [Source:UniProtKB/Swiss-Prot;Acc:O02056])

HSP 1 Score: 328.561 bits (841), Expect = 1.497e-110
Identity = 175/350 (50.00%), Postives = 240/350 (68.57%), Query Frame = 2
Query:  116 SRPLINVYKVNLPTDDSKPKLECIIDGSVKFPSVFNAPLRMDILSSVHDLIRKNSRQPYAVSTGAGHNNSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGHMFAPTKVWRKWHHRVPIQQRRYAMVTAIAATGYTPLVAARGHKVEKVPELPLVISEEYEGLKKTKMAVKVLKGCGVWPDIEKVKDSRKVRAGRGKMRNRRYVQKCGPIVIYKSDNGITKAFRNIPGVTXXXXXXXXXXXXSPGGHMGRLTIWTKPAIEELEKIYGSW-KTASQSKVDFLLPAPTMKNADLMRIINSEEIQSVLKARNMXXXXXXXXXXXXXXXNDGVMAKLNPYAAGEKKINK 1162
            +RPL+ VY        S+          ++ P+VF  P+R D++S + D +R+N RQ +AV+T AG  +SAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGHMFAP KV+R+WH  V I Q+RYA+ +AIAA+G   L+ ARGH +++V E+PLV+S++ E  +KTK AV  L+   +W DIEKV +S++ RAG+GK+RNR++ QK GP+VIY  D    +AFRNIPGV ++NV+ LN+LKL+PGGH+GRL IWT+ A ++L+ IYG+    +SQ K  + +P P M N+D  RII SEE+   ++A                 +   ++ KLNPYA+  +K +K
Sbjct:    3 ARPLVTVYDEKYEATQSQ----------IRLPAVFRTPIRPDLVSFIADQVRRNRRQAHAVNTKAGKQHSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGHMFAPLKVFRRWHRNVNIAQKRYAVSSAIAASGIPALLQARGHVIDQVAEVPLVVSDKVESFRKTKEAVVFLRRSHLWADIEKVYNSKRNRAGKGKLRNRQHKQKLGPVVIYGQDAECARAFRNIPGVDVMNVERLNLLKLAPGGHLGRLIIWTESAFKKLDTIYGTTVANSSQLKKGWSVPLPIMANSDFSRIIRSEEVVKAIRAPKKNPVLPKVHRNPLKKRT--LLYKLNPYASILRKASK 340          
BLAST of 60S ribosomal protein L4 vs. Ensembl Fly
Match: RpL4 (gene:FBgn0003279 transcript:FBtr0085248)

HSP 1 Score: 339.732 bits (870), Expect = 6.484e-114
Identity = 183/341 (53.67%), Postives = 243/341 (71.26%), Query Frame = 2
Query:  116 SRPLINVYKVNLPTDDSKPKLECIIDGSVKFPSVFNAPLRMDILSSVHDLIRKNSRQPYAVSTGAGHNNSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGHMFAPTKVWRKWHHRVPIQQRRYAMVTAIAATGYTPLVAARGHKVEKVPELPLVISEEYEGLKKTKMAVKVLKGCGVWPDIEKVKDSRKVRAGRGKMRNRRYVQKCGPIVIYKSDNGITKAFRNIPGVTXXXXXXXXXXXXSPGGHMGRLTIWTKPAIEELEKIYGSWKTASQSKVDFLLPAPTMKNADLMRIINSEEIQSVLKARNMXXXXXXXXXXXXXXXNDGVMAKLNPYA 1138
            +RPL++VY     T+ ++P      D ++  P+VF AP+R D+++ VH L+R+N+RQ YAVS  AGH  SAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGG MFAPTK +R+WH +V + QRRYA+V+AIAA+G   LV ++GH ++ V E PLV+S+E + ++KTK AV  L+   +W DI+KV  S++ RAGRG MR+RR + + GP+V+Y  D G+ KAFRNIPG+  INV  LN+LKL+PGGH+GR  IWT+ A   L  ++G+WK  S  K  + LP P M N DL R++ SEEI+ VL  R+ + +   S  + NPL N   + KLNPYA
Sbjct:    6 ARPLVSVY-----TEKNEPAK----DKNICLPAVFKAPIRPDVVNEVHQLLRRNNRQAYAVSELAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKTFRRWHRKVNVNQRRYALVSAIAASGVPALVQSKGHVIDGVSEFPLVVSDEVQKVQKTKQAVIFLRRLKIWADIQKVYKSQRFRAGRGTMRDRRRIARRGPLVVYDKDEGLRKAFRNIPGIETINVDKLNLLKLAPGGHVGRFVIWTESAFARLNDLFGTWKKPSTLKKGYNLPQPKMANTDLSRLLKSEEIRKVL--RDPRKRVFRSVRRLNPLTNVRQLIKLNPYA 335          
BLAST of 60S ribosomal protein L4 vs. Ensembl Zebrafish
Match: rpl4 (ribosomal protein L4 [Source:ZFIN;Acc:ZDB-GENE-030131-9034])

HSP 1 Score: 368.622 bits (945), Expect = 1.330e-125
Identity = 196/343 (57.14%), Postives = 247/343 (72.01%), Query Frame = 2
Query:  110 AMSRPLINVYKVNLPTDDSKPKLECIIDGSVKFPSVFNAPLRMDILSSVHDLIRKNSRQPYAVSTGAGHNNSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGHMFAPTKVWRKWHHRVPIQQRRYAMVTAIAATGYTPLVAARGHKVEKVPELPLVISEEYEGLKKTKMAVKVLKGCGVWPDIEKVKDSRKVRAGRGKMRNRRYVQKCGPIVIYKSDNGITKAFRNIPGVTXXXXXXXXXXXXSPGGHMGRLTIWTKPAIEELEKIYGSWKTASQSKVDFLLPAPTMKNADLMRIINSEEIQSVLKARNMXXXXXXXXXXXXXXXNDGVMAKLNPYA 1138
            A +RPLI+VY     T             +V  P+VF AP+R DI++ VH  +RKN RQPYAVS  AGH  SAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGG MFAPTK WR+WH RV + Q+RYAM +A+AA+    LV A+GH++E++PE+PLV+ ++ EG KKTK AV +LK    W DI+KV  S+++RAG+GKMRNRR +Q+ GP +IY  DNG+T+AFRNIPG+TL +V  L++LKL+PGGH+GR  IWT+ A  +L+ +YG+W+ AS  KVD+ LP   M   DL RII SEE+Q  ++  N   +      KKNPLKN  VM KLNPYA
Sbjct:    2 ACARPLISVYSEKGETSGK----------NVVMPAVFRAPIRPDIVNFVHTNMRKNKRQPYAVSELAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKTWRRWHRRVNVNQKRYAMCSALAASALPSLVMAKGHRIEEIPEVPLVVDDKVEGYKKTKEAVLLLKKLKAWNDIKKVYASQRMRAGKGKMRNRRRIQRKGPCIIYNEDNGVTRAFRNIPGITLQSVSRLDLLKLAPGGHIGRFCIWTEGAFRKLDDLYGTWRKASTLKVDYNLPMHKMSITDLNRIIKSEEVQKAIRPAN--RKINRRVFKKNPLKNLRVMMKLNPYA 332          
BLAST of 60S ribosomal protein L4 vs. Ensembl Xenopus
Match: rpl4 (ribosomal protein L4 [Source:NCBI gene;Acc:100327250])

HSP 1 Score: 340.117 bits (871), Expect = 6.067e-114
Identity = 193/345 (55.94%), Postives = 252/345 (73.04%), Query Frame = 2
Query:  104 SKAMSRPLINVYKVNLPTDDSKPKLECIIDGSVKFPSVFNAPLRMDILSSVHDLIRKNSRQPYAVSTGAGHNNSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGHMFAPTKVWRKWHHRVPIQQRRYAMVTAIAATGYTPLVAARGHKVEKVPELPLVISEEYEGLKKTKMAVKVLKGCGVWPDIEKVKDSRKVRAGRGKMRNRRYVQKCGPIVIYKSDNGITKAFRNIPGVTXXXXXXXXXXXXSPGGHMGRLTIWTKPAIEELEKIYGSWKTASQSKVDFLLPAPTMKNADLMRIINSEEIQSVLKARNMXXXXXXXXXXXXXXXNDGVMAKLNPYA 1138
            S A +RPLI+VY     T             +V  P+VF AP+R DI++ VH  +RKN+RQPYAVS  AGH  SAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGG MFAPTK WR+WH RV   Q+RYA+ +A+AA+    LV ++GH++E++PE+PLV+ ++ E  KKTK AV +LK    W DI+KV  S+++RAG+GKMRNRR +Q+ GP V+Y  +NGI KAFRNIPG+TL+NV  LN+L+L+PGGH+GR  IWT+ A  +L+ +YG+W+ +++ K D+ LP   M N DL RI+ S+EIQ  L+A N   + K  ++KKNPLKN  +M +LNPYA
Sbjct:    4 SMACARPLISVYSEKGETSGK----------NVTMPAVFKAPIRPDIVNFVHTNLRKNNRQPYAVSELAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKTWRRWHRRVNTTQKRYAVCSALAASALPALVMSKGHRIEEIPEVPLVVEDKVESYKKTKEAVLLLKKLKAWNDIKKVYASQRMRAGKGKMRNRRRIQRRGPCVVYNENNGIVKAFRNIPGITLLNVSKLNLLRLAPGGHVGRFCIWTESAFRKLDDLYGTWRKSAKLKADYNLPMHKMTNTDLTRILKSQEIQRALRAPN--KKVKRRELKKNPLKNLRIMMRLNPYA 336          
BLAST of 60S ribosomal protein L4 vs. Ensembl Mouse
Match: Rpl4 (ribosomal protein L4 [Source:MGI Symbol;Acc:MGI:1915141])

HSP 1 Score: 338.961 bits (868), Expect = 3.101e-113
Identity = 196/343 (57.14%), Postives = 250/343 (72.89%), Query Frame = 2
Query:  110 AMSRPLINVYKVNLPTDDSKPKLECIIDGSVKFPSVFNAPLRMDILSSVHDLIRKNSRQPYAVSTGAGHNNSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGHMFAPTKVWRKWHHRVPIQQRRYAMVTAIAATGYTPLVAARGHKVEKVPELPLVISEEYEGLKKTKMAVKVLKGCGVWPDIEKVKDSRKVRAGRGKMRNRRYVQKCGPIVIYKSDNGITKAFRNIPGVTXXXXXXXXXXXXSPGGHMGRLTIWTKPAIEELEKIYGSWKTASQSKVDFLLPAPTMKNADLMRIINSEEIQSVLKARNMXXXXXXXXXXXXXXXNDGVMAKLNPYA 1138
            A +RPLI+VY     +             +V  P+VF AP+R DI++ VH  +RKN+RQPYAVS  AGH  SAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGG MFAPTK WR+WH RV   Q+RYA+ +A+AA+    LV ++GH++E+VPELPLV+ ++ EG KKTK AV++LK    W DI+KV  S+++RAG+GKMRNRR +Q+ GP +IY  DNGI KAFRNIPG+TL+NV  LNILKL+PGGH+GR  IWT+ A  +L+++YG+W+ A+  K ++ LP   M N DL RI+ S EIQ  L+A   + +     +KKNPLKN  +M KLNPYA
Sbjct:    2 ACARPLISVYSEKGESSGK----------NVTLPAVFKAPIRPDIVNFVHTNLRKNNRQPYAVSELAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKTWRRWHRRVNTTQKRYAICSALAASALPALVMSKGHRIEEVPELPLVVEDKVEGYKKTKEAVQLLKKLKAWNDIKKVYASQRMRAGKGKMRNRRRIQRRGPCIIYNEDNGIIKAFRNIPGITLLNVSKLNILKLAPGGHVGRFCIWTESAFRKLDELYGTWRKAASLKSNYNLPMHKMMNTDLSRILKSPEIQRALRA--PRKKIHRRVLKKNPLKNLRIMLKLNPYA 332          
BLAST of 60S ribosomal protein L4 vs. UniProt/SwissProt
Match: sp|P49165|RL4_URECA (60S ribosomal protein L4 OS=Urechis caupo OX=6431 GN=RPL4 PE=2 SV=1)

HSP 1 Score: 369.007 bits (946), Expect = 1.080e-124
Identity = 194/340 (57.06%), Postives = 249/340 (73.24%), Query Frame = 2
Query:  116 SRPLINVYKVNLPTDDSKPKLECIIDGSVKFPSVFNAPLRMDILSSVHDLIRKNSRQPYAVSTGAGHNNSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGHMFAPTKVWRKWHHRVPIQQRRYAMVTAIAATGYTPLVAARGHKVEKVPELPLVISEEYEGLKKTKMAVKVLKGCGVWPDIEKVKDSRKVRAGRGKMRNRRYVQKCGPIVIYKSDNGITKAFRNIPGVTXXXXXXXXXXXXSPGGHMGRLTIWTKPAIEELEKIYGSWKTASQSKVDFLLPAPTMKNADLMRIINSEEIQSVLKARNMXXXXXXXXXXXXXXXNDGVMAKLNPY 1135
            +RPLI V+       +S          +V  P+VF AP+R DI++ VH  ++KN RQPYAVS  AGH  SAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRG  MFAPTK WR+WH RV   QRR+A+V+AIAATG   +V ++GH +E++PE+PLV+S+  E +KKTK AV VL+    WPD+EKVK+SR+ RAG+GK+RNRR +Q+ GP++IY +DNGI++AFRNIPG+TL+NV  LN+L ++PGGH+GR  IWT+ A ++L+ IYG+WK  S  K  + LP P M N DL R++ S+EIQ+ L  R  K  K     KKNPLKN  VM +LNP+
Sbjct:    3 ARPLITVHSDKGAASES----------NVTLPAVFRAPIRPDIVNFVHFELKKNGRQPYAVSQKAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGRRMFAPTKTWRRWHRRVNTTQRRHALVSAIAATGIPAVVMSKGHCIEQIPEVPLVVSDSVESIKKTKEAVVVLRRLKAWPDVEKVKNSRRFRAGKGKLRNRRAIQRRGPLIIYGTDNGISRAFRNIPGITLVNVNRLNLLSMAPGGHVGRFCIWTESAFKQLDNIYGTWKRPSAEKSHYNLPMPKMTNTDLSRLLKSDEIQNAL--REPKKDKARRLQKKNPLKNYRVMMRLNPF 330          
BLAST of 60S ribosomal protein L4 vs. UniProt/SwissProt
Match: sp|Q9SF40|RL4A_ARATH (60S ribosomal protein L4-1 OS=Arabidopsis thaliana OX=3702 GN=RPL4A PE=1 SV=1)

HSP 1 Score: 357.451 bits (916), Expect = 6.620e-120
Identity = 197/353 (55.81%), Postives = 253/353 (71.67%), Query Frame = 2
Query:  110 AMSRPLINVYKVN--LPTDDSKPKLECIIDGSVKFPSVFNAPLRMDILSSVHDLIRKNSRQPYAVSTGAGHNNSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGHMFAPTKVWRKWHHRVPIQQRRYAMVTAIAATGYTPLVAARGHKVEKVPELPLVISEEYEGLKKTKMAVKVLKGCGVWPDIEKVKDSRKVRAGRGKMRNRRYVQKCGPIVIYKSDNG-ITKAFRNIPGVTXXXXXXXXXXXXSPGGHMGRLTIWTKPAIEELEKIYGSWKTASQSKVDFLLPAPTMKNADLMRIINSEEIQSVLKARNMXXXXXXXXXXXXXXXNDGVMAKLNPYAAGEKKIN 1159
            A +RPL+ +  ++  + TD S          +V  P V  AP+R DI++ VH  I  NSRQPYAVS  AGH  SAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGG MFAPTK+WR+WH RV +  +R+A+V+AIAAT    LV ARGHK+E VPE+PLV+S+  E ++KT  A+KVLK  G + D EK K+S  +R G+GKMRNRRY+ + GP+V+Y ++   I KAFRN+PGV L +V+ LN+LKL+PGGH+GR  IWTK A E+LE IYGS++  S+ K  ++LP   M NADL RIINS+EIQSV+    +K   K + +KKNPLKN  VM KLNPYA   K+++
Sbjct:    4 AAARPLVTIQTLDGDMSTDQS---------STVVLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVSKKAGHQTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRVNVNMKRHAIVSAIAATAVPALVMARGHKIENVPEMPLVVSDSAEAVEKTSAAIKVLKQIGAYDDAEKAKNSIGIRPGKGKMRNRRYISRKGPLVVYGTEGSKIVKAFRNLPGVELCHVERLNLLKLAPGGHLGRFVIWTKSAFEKLESIYGSFEKPSEKKKGYVLPRAKMVNADLARIINSDEIQSVVNP--IKKDAKRAVLKKNPLKNLNVMLKLNPYAKTAKRMS 345          
BLAST of 60S ribosomal protein L4 vs. UniProt/SwissProt
Match: sp|P49691|RL4B_ARATH (60S ribosomal protein L4-2 OS=Arabidopsis thaliana OX=3702 GN=RPL4D PE=1 SV=2)

HSP 1 Score: 355.525 bits (911), Expect = 3.978e-119
Identity = 196/355 (55.21%), Postives = 254/355 (71.55%), Query Frame = 2
Query:  104 SKAMSRPLINVYKVN--LPTDDSKPKLECIIDGSVKFPSVFNAPLRMDILSSVHDLIRKNSRQPYAVSTGAGHNNSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGHMFAPTKVWRKWHHRVPIQQRRYAMVTAIAATGYTPLVAARGHKVEKVPELPLVISEEYEGLKKTKMAVKVLKGCGVWPDIEKVKDSRKVRAGRGKMRNRRYVQKCGPIVIYKSDNG-ITKAFRNIPGVTXXXXXXXXXXXXSPGGHMGRLTIWTKPAIEELEKIYGSWKTASQSKVDFLLPAPTMKNADLMRIINSEEIQSVLKARNMXXXXXXXXXXXXXXXNDGVMAKLNPYAAGEKKIN 1159
            + A +RPL+ V  ++  + TD S          +V  P V  AP+R DI++ VH  I  NSRQPYAVS  AGH  SAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGG MFAPTK+WR+WH RV +  +R+A+V+AIAAT    LV ARGHK+E VPE+PLV+S+  E ++KT  A+KVLK  G + D EK K+S  +R G+GKMRNRRY+ + GP+V++ ++   I KAFRN+PGV L +V+ LN+LKL+PGGH+GR  IWTK A E+LE IYGS++  S+ K  ++LP   M NADL RIINS+E+QSV+    +K   K + +KKNPLKN  VM KLNPYA   K+++
Sbjct:    3 ASAAARPLVTVQGLDGDMSTDQST---------TVTLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVSKKAGHQTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRVNVNMKRHAIVSAIAATAVPALVMARGHKIENVPEMPLVVSDSAEAVEKTSAAIKVLKQIGAYDDAEKAKNSIGIRPGKGKMRNRRYISRKGPLVVFGTEGAKIVKAFRNLPGVELCHVERLNLLKLAPGGHLGRFVIWTKSAFEKLESIYGSFEKPSEKKKGYVLPRAKMVNADLARIINSDEVQSVVNP--IKDGSKRAVLKKNPLKNLNVMFKLNPYAKTAKRMS 346          
BLAST of 60S ribosomal protein L4 vs. UniProt/SwissProt
Match: sp|P35679|RL4A_SCHPO (60S ribosomal protein L4-A OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=rpl402 PE=1 SV=2)

HSP 1 Score: 353.599 bits (906), Expect = 6.910e-119
Identity = 183/347 (52.74%), Postives = 247/347 (71.18%), Query Frame = 2
Query:  110 AMSRPLINVYKVNLPTDDSKPKLECIIDGSVKFPSVFNAPLRMDILSSVHDLIRKNSRQPYAVSTGAGHNNSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGHMFAPTKVWRKWHHRVPIQQRRYAMVTAIAATGYTPLVAARGHKVEKVPELPLVISEEYEGLKKTKMAVKVLKGCGVWPDIEKVKDSRKVRAGRGKMRNRRYVQKCGPIVIYKSDNGITKAFRNIPGVTXXXXXXXXXXXXSPGGHMGRLTIWTKPAIEELEKIYGSWKTASQSKVDFLLPAPTMKNADLMRIINSEEIQSVLKARNMXXXXXXXXXXXXXXXNDGVMAKLNPYAAGEK 1150
            A +RP +++Y           K   +   ++  P VF AP+R D++ SVH  + KN RQPYAVS  AGH  SAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR G MFAPTK WRKWH +V   ++RYA+ +A+AA+G   L+ ARGH++E++PE+PLV+ +  +  +KTK AV +LK    + D+ KV +SRK+RAG+GK+RNRR+VQ+ GP+V++  D GI KAFRNIPGV ++NV+ LN+L+L+PGGH+GR  IWTK A   L+ ++GS   A+Q K ++ LP   + NAD+ R+INS+EIQS++KA      K++   KKNPLKN  V+A+LNPYA   K
Sbjct:    2 AAARPTVSIYS----------KDGSVSSETIALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEKAGHQTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTKTWRKWHVKVNQNEKRYAISSAVAASGVPSLLLARGHRIEEIPEVPLVVDDAVQSFQKTKEAVALLKEIKAYRDVVKVANSRKLRAGKGKLRNRRHVQRRGPLVVFNEDAGIVKAFRNIPGVEIVNVRRLNLLQLAPGGHLGRFVIWTKSAFGLLDSVFGSTTEAAQLKKNYFLPENIISNADVTRLINSDEIQSIVKAAGPSRVKRAHVQKKNPLKNKAVLARLNPYAKAYK 338          
BLAST of 60S ribosomal protein L4 vs. UniProt/SwissProt
Match: sp|Q9P784|RL4B_SCHPO (60S ribosomal protein L4-B OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=rpl401 PE=3 SV=1)

HSP 1 Score: 353.214 bits (905), Expect = 8.496e-119
Identity = 185/357 (51.82%), Postives = 251/357 (70.31%), Query Frame = 2
Query:  110 AMSRPLINVYKVNLPTDDSKPKLECIIDGSVKFPSVFNAPLRMDILSSVHDLIRKNSRQPYAVSTGAGHNNSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGHMFAPTKVWRKWHHRVPIQQRRYAMVTAIAATGYTPLVAARGHKVEKVPELPLVISEEYEGLKKTKMAVKVLKGCGVWPDIEKVKDSRKVRAGRGKMRNRRYVQKCGPIVIYKSDNGITKAFRNIPGVTXXXXXXXXXXXXSPGGHMGRLTIWTKPAIEELEKIYGSWKTASQSKVDFLLPAPTMKNADLMRIINSEEIQSVLKARNMXXXXXXXXXXXXXXXNDGVMAKLNPYAAGEK---KINKRKS 1171
            A +RP +++Y           K   +   ++  P VF AP+R D++ SVH  + KN RQPYAVS  AGH  SAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR G MFAPTK WRKWH +V   ++RYA+ +A+AA+G   L+ ARGH++E++PE+PLV+ +  +  +KTK AV +LK    + D+ KV +SRK+RAG+GK+RNRR+VQ+ GP+V++  D GI KAFRNIPGV ++NV+ LN+L+L+PGGH+GR  IWTK A   L+ ++GS    +Q K ++ LP   + NAD+ R+INS+EIQS++KA      K++   KKNPLKN  V+++LNPYA   K   KIN  K+
Sbjct:    2 AAARPTVSIYN----------KDGSVSSETLALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEKAGHQTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTKTWRKWHVKVNQNEKRYAIASAVAASGVPSLLLARGHRIEEIPEVPLVVDDAVQSFQKTKEAVALLKEIKAYRDVIKVANSRKLRAGKGKLRNRRHVQRRGPLVVFNEDTGIVKAFRNIPGVEIVNVRRLNLLQLAPGGHLGRFVIWTKSAFGLLDSVFGSTTEVAQLKKNYFLPENIISNADVTRLINSDEIQSIVKAAGPSRVKRAHVQKKNPLKNKAVLSRLNPYAKAYKANVKINSEKT 348          
BLAST of 60S ribosomal protein L4 vs. TrEMBL
Match: A0A504YWQ0 (Ribosomal protein l4 OS=Fasciola gigantica OX=46835 GN=FGIG_09016 PE=4 SV=1)

HSP 1 Score: 426.017 bits (1094), Expect = 1.454e-144
Identity = 226/347 (65.13%), Postives = 272/347 (78.39%), Query Frame = 2
Query:  110 AMSRPLINVYKVNLPTDDSKPKLECIIDGSVKFPSVFNAPLRMDILSSVHDLIRKNSRQPYAVSTGAGHNNSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGHMFAPTKVWRKWHHRVPIQQRRYAMVTAIAATGYTPLVAARGHKVEKVPELPLVISEEYEGLKKTKMAVKVLKGCGVWPDIEKVKDSRKVRAGRGKMRNRRYVQKCGPIVIYKSDNGITKAFRNIPGVTXXXXXXXXXXXXSPGGHMGRLTIWTKPAIEELEKIYGSWKTASQSKVDFLLPAPTMKNADLMRIINSEEIQSVLKARNMXXXXXXXXXXXXXXXNDGVMAKLNPYAAGEK 1150
            A++RP++NV+K     D S   +      +VK P+VF+APLR D+L++VHD +RKNSRQPYAVS  AGH  SAESWGTGRAVARIPRV GGGTHRSGQGAFGNMCRGGHMFAPTKVWR WH R+ +QQRRYAM +AIAATG   LV ARGH++++VPELPLV+S++ E +KKTK AVK+LK    W DI+KV +S++ RAGRGKMRNRR +QK GPI+IY+ D+G+T+AFRNIPGVTLINV  LNILKL+PGGHMGRL IWT  A  +L+KIYG+WKT S  K DF LP P M NADL R+++  E+ SVL+ R M  Q+   KVKKNPLKN   + KLNPYA GEK
Sbjct:    2 AVTRPIVNVFKA----DGSSSGM------TVKMPAVFSAPLRSDLLNTVHDQMRKNSRQPYAVSAKAGHQTSAESWGTGRAVARIPRVHGGGTHRSGQGAFGNMCRGGHMFAPTKVWRHWHRRINVQQRRYAMCSAIAATGVPALVMARGHRIDRVPELPLVLSDDIESIKKTKEAVKILKSIKAWSDIQKVYNSQRFRAGRGKMRNRRRIQKRGPIIIYRKDDGLTRAFRNIPGVTLINVSALNILKLAPGGHMGRLAIWTAGAFRQLDKIYGTWKTPSVKKTDFNLPMPKMTNADLGRLLHCREVTSVLRPRLMHFQRPGPKVKKNPLKNMQALLKLNPYAIGEK 338          
BLAST of 60S ribosomal protein L4 vs. TrEMBL
Match: A0A5J4P0I8 (Large subunit ribosomal protein L4e OS=Paragonimus westermani OX=34504 GN=DEA37_0014265 PE=4 SV=1)

HSP 1 Score: 425.631 bits (1093), Expect = 1.722e-144
Identity = 229/358 (63.97%), Postives = 273/358 (76.26%), Query Frame = 2
Query:  110 AMSRPLINVYKVNLPTDDSKPKLECIIDGSVKFPSVFNAPLRMDILSSVHDLIRKNSRQPYAVSTGAGHNNSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGHMFAPTKVWRKWHHRVPIQQRRYAMVTAIAATGYTPLVAARGHKVEKVPELPLVISEEYEGLKKTKMAVKVLKGCGVWPDIEKVKDSRKVRAGRGKMRNRRYVQKCGPIVIYKSDNGITKAFRNIPGVTXXXXXXXXXXXXSPGGHMGRLTIWTKPAIEELEKIYGSWKTASQSKVDFLLPAPTMKNADLMRIINSEEIQSVLKARNMXXXXXXXXXXXXXXXNDGVMAKLNPYAAGEKKINKRKSCGID 1183
            A++RP +NV+KV     D  P        +VK PSVF APLR D+L+ VHD +RKNSRQPYAVS  AGH  SAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGHMFAPTKVWR+WH R+ +QQRRYAM TAIAATG   LV ARGH+++++PE+PLVIS+E E +KKTK AVK+LK    W DI+KV +S++ RAGRGKMRNRR +QK GPIVI+  DNGIT+AFRNIPGVTLINV  LN+LKL+PGGHMGRL IW+  A  +L+K+YG+WKTAS  K  F LP   M N+DL R+++  E+ SVLK R M  Q+   KVKKNPLKN   + KLNP+A GEK + K +   I 
Sbjct:    2 AVARPTVNVFKV-----DGAPS-----GMTVKMPSVFTAPLRSDLLNFVHDQMRKNSRQPYAVSAKAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGHMFAPTKVWRRWHRRINVQQRRYAMCTAIAATGVPALVMARGHRIDRLPEVPLVISDEVESVKKTKEAVKILKAVKAWSDIQKVYNSQRFRAGRGKMRNRRRIQKRGPIVIFHKDNGITRAFRNIPGVTLINVNALNLLKLAPGGHMGRLAIWSAGAFRQLDKLYGTWKTASIKKTGFHLPMAKMTNSDLGRLLHCREVTSVLKPRRMHCQRPGPKVKKNPLKNMQALLKLNPFAIGEKNLRKARQLTIQ 349          
BLAST of 60S ribosomal protein L4 vs. TrEMBL
Match: A0A4E0RYD3 (Ribosomal protein l4 OS=Fasciola hepatica OX=6192 GN=D915_007907 PE=4 SV=1)

HSP 1 Score: 425.631 bits (1093), Expect = 2.084e-144
Identity = 226/347 (65.13%), Postives = 272/347 (78.39%), Query Frame = 2
Query:  110 AMSRPLINVYKVNLPTDDSKPKLECIIDGSVKFPSVFNAPLRMDILSSVHDLIRKNSRQPYAVSTGAGHNNSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGHMFAPTKVWRKWHHRVPIQQRRYAMVTAIAATGYTPLVAARGHKVEKVPELPLVISEEYEGLKKTKMAVKVLKGCGVWPDIEKVKDSRKVRAGRGKMRNRRYVQKCGPIVIYKSDNGITKAFRNIPGVTXXXXXXXXXXXXSPGGHMGRLTIWTKPAIEELEKIYGSWKTASQSKVDFLLPAPTMKNADLMRIINSEEIQSVLKARNMXXXXXXXXXXXXXXXNDGVMAKLNPYAAGEK 1150
            A++RP++NV+K     D S   +      +VK P+VF+APLR D+L++VHD +RKNSRQPYAVS  AGH  SAESWGTGRAVARIPRV GGGTHRSGQGAFGNMCRGGHMFAPTKVWR WH R+ +QQRRYAM +AIAATG   LV ARGH++++VPELPLV+S++ E +KKTK AVK+LK    W DI+KV +S++ RAGRGKMRNRR +QK GPI+IY+ D+G+T+AFRNIPGVTLINV  LNILKL+PGGHMGRL IWT  A  +L+KIYG+WKT S  K DF LP P M NADL R+++  E+ SVL+ R M  Q+   KVKKNPLKN   + KLNPYA GEK
Sbjct:    2 AVTRPIVNVFKA----DGSSSGM------TVKMPAVFSAPLRSDLLNTVHDQMRKNSRQPYAVSAKAGHQTSAESWGTGRAVARIPRVHGGGTHRSGQGAFGNMCRGGHMFAPTKVWRHWHRRINVQQRRYAMCSAIAATGVPALVMARGHRIDRVPELPLVLSDDIESIKKTKEAVKILKSIKAWSDIQKVYNSQRFRAGRGKMRNRRRIQKRGPIIIYRKDDGLTRAFRNIPGVTLINVSALNILKLAPGGHMGRLAIWTAGAFRQLDKIYGTWKTPSVKKTDFNLPMPKMTNADLGRLLHCREVTSVLRPRLMHFQRPGPKVKKNPLKNMQALLKLNPYAIGEK 338          
BLAST of 60S ribosomal protein L4 vs. TrEMBL
Match: A0A1S8WLA0 (Ribosomal protein, L4/L1 family OS=Opisthorchis viverrini OX=6198 GN=X801_09014 PE=4 SV=1)

HSP 1 Score: 420.624 bits (1080), Expect = 2.114e-142
Identity = 222/350 (63.43%), Postives = 273/350 (78.00%), Query Frame = 2
Query:  107 KAMSRPLINVYKVNLPTDDSKPKLECIIDGSVKFPSVFNAPLRMDILSSVHDLIRKNSRQPYAVSTGAGHNNSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGHMFAPTKVWRKWHHRVPIQQRRYAMVTAIAATGYTPLVAARGHKVEKVPELPLVISEEYEGLKKTKMAVKVLKGCGVWPDIEKVKDSRKVRAGRGKMRNRRYVQKCGPIVIYKSDNGITKAFRNIPGVTXXXXXXXXXXXXSPGGHMGRLTIWTKPAIEELEKIYGSWKTASQSKVDFLLPAPTMKNADLMRIINSEEIQSVLKARNMXXXXXXXXXXXXXXXNDGVMAKLNPYAAGEKKI 1156
            +A+ RP++NV+KV     D  P        ++K P+VF+APLR D+L+ VHD +RKNSRQPYAVS  AGH  SAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGHMFAPTKVWR+WH R+ +QQRRYAM +AIAATG   LV ARGH+++++PE+PLVIS+E E +KKTK AVKVLK    W DI+KV +S++ RAGRGKMRNRR +QK GPI+I++ D+GIT+AFRNIPGVTLINV  LN+LKL+PGGHMGRL IW+  A  +L+K+YG+WKT S  K  F LP P M N+DL R+++  E+ SVLK R +  Q+   KVKKNPLKN   + KLNP+A GEK +
Sbjct:    6 RAVCRPIVNVFKV-----DGAPS-----GMTIKMPAVFSAPLRSDLLNFVHDQMRKNSRQPYAVSAKAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGHMFAPTKVWRRWHKRINVQQRRYAMCSAIAATGVPALVMARGHRIDRLPEVPLVISDEIESVKKTKEAVKVLKSVKAWSDIQKVYNSQRFRAGRGKMRNRRRIQKRGPIIIFRKDDGITRAFRNIPGVTLINVNVLNLLKLAPGGHMGRLAIWSASAFRQLDKLYGTWKTPSLKKSGFHLPKPKMTNSDLGRLLHCREVTSVLKPRRLHLQRPGPKVKKNPLKNIQALLKLNPFAIGEKNM 345          
BLAST of 60S ribosomal protein L4 vs. TrEMBL
Match: A0A4S2LI53 (Ribos_L4_asso_C domain-containing protein OS=Opisthorchis felineus OX=147828 GN=CRM22_008495 PE=4 SV=1)

HSP 1 Score: 417.927 bits (1073), Expect = 2.147e-141
Identity = 221/349 (63.32%), Postives = 272/349 (77.94%), Query Frame = 2
Query:  110 AMSRPLINVYKVNLPTDDSKPKLECIIDGSVKFPSVFNAPLRMDILSSVHDLIRKNSRQPYAVSTGAGHNNSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGHMFAPTKVWRKWHHRVPIQQRRYAMVTAIAATGYTPLVAARGHKVEKVPELPLVISEEYEGLKKTKMAVKVLKGCGVWPDIEKVKDSRKVRAGRGKMRNRRYVQKCGPIVIYKSDNGITKAFRNIPGVTXXXXXXXXXXXXSPGGHMGRLTIWTKPAIEELEKIYGSWKTASQSKVDFLLPAPTMKNADLMRIINSEEIQSVLKARNMXXXXXXXXXXXXXXXNDGVMAKLNPYAAGEKKI 1156
            A+ RP++NV+KV     D  P        ++K P+VF+APLR D+L+ VHD +RKNSRQPYAVS  AGH  SAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGHMFAPTKVWR+WH R+ +QQRRYAM +AIAATG   LV ARGH+++++PE+PLVIS+E E +KKTK AVKVLK    W DI+KV +S++ RAGRGKMRNRR +QK GPI+I++ D+GIT+AFRNIPGVTLINV  LN+LKL+PGGHMGRL +W+  A  +L+K+YG+WKT S  K  F LP P M N+DL R+++  E+ SVLK R +  Q+   KVKKNPLKN   + KLNP+A GEK +
Sbjct:    2 AVCRPIVNVFKV-----DGAPS-----GMTIKMPAVFSAPLRSDLLNFVHDQMRKNSRQPYAVSAKAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGHMFAPTKVWRRWHKRINVQQRRYAMCSAIAATGVPALVMARGHRIDRLPEVPLVISDEIESVKKTKEAVKVLKSVKAWSDIQKVYNSQRFRAGRGKMRNRRRIQKRGPIIIFRKDDGITRAFRNIPGVTLINVNVLNLLKLAPGGHMGRLALWSAGAFRQLDKLYGTWKTPSLKKSGFHLPKPKMTNSDLGRLLHCREVTSVLKPRRLHLQRPGPKVKKNPLKNIQALLKLNPFAIGEKNM 340          
BLAST of 60S ribosomal protein L4 vs. Ensembl Cavefish
Match: rpl4 (ribosomal protein L4 [Source:NCBI gene;Acc:103028337])

HSP 1 Score: 352.829 bits (904), Expect = 2.220e-119
Identity = 192/343 (55.98%), Postives = 249/343 (72.59%), Query Frame = 2
Query:  110 AMSRPLINVYKVNLPTDDSKPKLECIIDGSVKFPSVFNAPLRMDILSSVHDLIRKNSRQPYAVSTGAGHNNSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGHMFAPTKVWRKWHHRVPIQQRRYAMVTAIAATGYTPLVAARGHKVEKVPELPLVISEEYEGLKKTKMAVKVLKGCGVWPDIEKVKDSRKVRAGRGKMRNRRYVQKCGPIVIYKSDNGITKAFRNIPGVTXXXXXXXXXXXXSPGGHMGRLTIWTKPAIEELEKIYGSWKTASQSKVDFLLPAPTMKNADLMRIINSEEIQSVLKARNMXXXXXXXXXXXXXXXNDGVMAKLNPYA 1138
            A +RPLI+VY     T             +V  P+VF AP+R D+++ VH  +RKN RQPYAVS  AGH  SAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGG MFAPTK WR+WH R+ + Q+RYA+ +A+AA+    LV A+GH++E++PE+PLV+ ++ EG KKTK AV +LK    W DI+KV  S+++RAG+GKMRNRR +Q+ GP +IY  DNG+T+AFRNIPG+TL  V  L++L+L+PGGH+GR  IWT+ A  +L+ +YG+W+ AS  KVD+ LP   M N DL R++ SEEI   L+A   K + +   +KKNPLKN  +M KLNPYA
Sbjct:    2 ACARPLISVYSEKGETSGK----------NVVMPAVFKAPIRPDVVNFVHTNMRKNKRQPYAVSELAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKTWRRWHRRINVTQKRYAICSALAASALPALVLAKGHRIEEIPEVPLVVDDKVEGYKKTKEAVLLLKKLKAWNDIKKVYASQRMRAGKGKMRNRRRIQRKGPCIIYNEDNGVTRAFRNIPGITLQQVGKLDLLRLAPGGHIGRFCIWTESAFRKLDDLYGTWRKASTLKVDYNLPMHKMTNTDLGRLLQSEEILKALRA--PKRKIQRRVLKKNPLKNLRIMMKLNPYA 332          
BLAST of 60S ribosomal protein L4 vs. Ensembl Sea Lamprey
Match: rpl4 (ribosomal protein L4 [Source:ZFIN;Acc:ZDB-GENE-030131-9034])

HSP 1 Score: 338.576 bits (867), Expect = 1.093e-113
Identity = 196/346 (56.65%), Postives = 255/346 (73.70%), Query Frame = 2
Query:  107 KAMSRPLINVYKVNLPTDDSKPKLECIIDGSVKFPSVFNAPLRMDILSSVHDLIRKNSRQPYAVSTGAGHNNSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGHMFAPTKVWRKWHHRVPIQQRRYAMVTAIAATGYTPLVAARGHKVEKVPELPLVISEEYEGLKKTKMAVKVLKGCGVWPDIEKVKDSRKVRAGRGKMRNRRYVQKCGPIVIYKSDNGITKAFRNIP--GVTXXXXXXXXXXXXSPGGHMGRLTIWTKPAIEELEKIYGSWKTASQSKVDFLLPAPTMKNADLMRIINSEEIQSVLKARNMXXXXXXXXXXXXXXXNDGVMAKLNPYA 1138
            +A +RPLI++Y       +S  K       +V  P+VF AP+R DI++ VH  +RKNSRQPYAVS  AGH  SAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGG MFAPTK WR+WH RV + Q+RYA+ +A+AAT    LV ++GH++E++PE+PLV+ ++ EG KKTK AV +LK    W D++KV  S+++RAG+GKMRNRR +Q+ GP +IY  DNG+T+AFRNIP  G+TL++VK LN+L+L+PGGH+GR  IWT+ A  +L+ IYG+W+  S+ K ++ LP   M N+DL RI+ SEEI  VL+  N   +     +KKNPLKN  VM KLNPYA
Sbjct:   13 QACARPLISIYS---EKGESSGK-------NVVMPAVFRAPIRPDIVNFVHTNLRKNSRQPYAVSVNAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKTWRRWHRRVNVTQKRYAICSALAATALPALVMSKGHRIEEIPEVPLVVEDKVEGYKKTKEAVLLLKKLKAWNDVKKVYASKRLRAGKGKMRNRRRIQRRGPCIIYNEDNGLTRAFRNIPGAGITLLSVKKLNLLRLAPGGHVGRFCIWTESAFSKLDDIYGTWRKPSKEKSNYNLPMNKMTNSDLSRILKSEEIHKVLRPPN--KKIVRRVLKKNPLKNLRVMIKLNPYA 346          
BLAST of 60S ribosomal protein L4 vs. Ensembl Yeast
Match: RPL4B (Ribosomal 60S subunit protein L4B; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4B has a paralog, RPL4A, that arose from the whole genome duplication [Source:SGD;Acc:S000002419])

HSP 1 Score: 293.123 bits (749), Expect = 4.240e-97
Identity = 184/343 (53.64%), Postives = 241/343 (70.26%), Query Frame = 2
Query:  113 MSRPLINVYKVN-LPTDDSKPKLECIIDGSVKFPSVFNAPLRMDILSSVHDLIRKNSRQPYAVSTGAGHNNSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGHMFAPTKVWRKWHHRVPIQQRRYAMVTAIAATGYTPLVAARGHKVEKVPELPLVISEEYEGLKKTKMAVKVLKGCGVWPDIEKVKDSRKVRAGRGKMRNRRYVQKCGPIVIYKSDNGITKAFRNIPGVTXXXXXXXXXXXXSPGGHMGRLTIWTKPAIEELEKIYGSWKTASQSKVDFLLPAPTMKNADLMRIINSEEIQSVLKARNMXXXXXXXXXXXXXXXNDGVMAKLNPYA 1138
            MSRP + V+ +    T ++ P            P+VF+AP+R DI+ +V   + KN RQ YAVS  AGH  SAESWGTGRAVARIPRV GGGT RSGQGAFGNMCRGG MFAPTK WRKW+ +V   ++RYA  +AIAAT    LV ARGH+VEK+PE+PLV+S + E ++KTK AV  LK  G   D+ KV  S+K+RAG+GK RNRR+ Q+ GP+V+Y  DNGI KA RN+PGV   NV +LN+L+L+PG H+GR  IWT+ A  +L++++GS +T + SKV + LP+  +  +D+ RIINS EIQS ++      QK++  +KKNPLKN  V+ +LNPYA
Sbjct:    1 MSRPQVTVHSLTGEATANALP-----------LPAVFSAPIRPDIVHTVFTSVNKNKRQAYAVSEKAGHQTSAESWGTGRAVARIPRVGGGGTGRSGQGAFGNMCRGGRMFAPTKTWRKWNVKVNHNEKRYATASAIAATAVASLVLARGHRVEKIPEIPLVVSTDLESIQKTKEAVAALKAVGAHSDLLKVLKSKKLRAGKGKYRNRRWTQRRGPLVVYAEDNGIVKALRNVPGVETANVASLNLLQLAPGAHLGRFVIWTEAAFTKLDQVWGS-ETVASSKVGYTLPSHIISTSDVTRIINSSEIQSAIRPAGQATQKRTHVLKKNPLKNKQVLLRLNPYA 331          
BLAST of 60S ribosomal protein L4 vs. Ensembl Yeast
Match: RPL4A (Ribosomal 60S subunit protein L4A; N-terminally acetylated; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4A has a paralog, RPL4B, that arose from the whole genome duplication [Source:SGD;Acc:S000000235])

HSP 1 Score: 293.123 bits (749), Expect = 4.624e-97
Identity = 184/343 (53.64%), Postives = 241/343 (70.26%), Query Frame = 2
Query:  113 MSRPLINVYKVN-LPTDDSKPKLECIIDGSVKFPSVFNAPLRMDILSSVHDLIRKNSRQPYAVSTGAGHNNSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGHMFAPTKVWRKWHHRVPIQQRRYAMVTAIAATGYTPLVAARGHKVEKVPELPLVISEEYEGLKKTKMAVKVLKGCGVWPDIEKVKDSRKVRAGRGKMRNRRYVQKCGPIVIYKSDNGITKAFRNIPGVTXXXXXXXXXXXXSPGGHMGRLTIWTKPAIEELEKIYGSWKTASQSKVDFLLPAPTMKNADLMRIINSEEIQSVLKARNMXXXXXXXXXXXXXXXNDGVMAKLNPYA 1138
            MSRP + V+ +    T ++ P            P+VF+AP+R DI+ +V   + KN RQ YAVS  AGH  SAESWGTGRAVARIPRV GGGT RSGQGAFGNMCRGG MFAPTK WRKW+ +V   ++RYA  +AIAAT    LV ARGH+VEK+PE+PLV+S + E ++KTK AV  LK  G   D+ KV  S+K+RAG+GK RNRR+ Q+ GP+V+Y  DNGI KA RN+PGV   NV +LN+L+L+PG H+GR  IWT+ A  +L++++GS +T + SKV + LP+  +  +D+ RIINS EIQS ++      QK++  +KKNPLKN  V+ +LNPYA
Sbjct:    1 MSRPQVTVHSLTGEATANALP-----------LPAVFSAPIRPDIVHTVFTSVNKNKRQAYAVSEKAGHQTSAESWGTGRAVARIPRVGGGGTGRSGQGAFGNMCRGGRMFAPTKTWRKWNVKVNHNEKRYATASAIAATAVASLVLARGHRVEKIPEIPLVVSTDLESIQKTKEAVAALKAVGAHSDLLKVLKSKKLRAGKGKYRNRRWTQRRGPLVVYAEDNGIVKALRNVPGVETANVASLNLLQLAPGAHLGRFVIWTEAAFTKLDQVWGS-ETVASSKVGYTLPSHIISTSDVTRIINSSEIQSAIRPAGQATQKRTHVLKKNPLKNKQVLLRLNPYA 331          
BLAST of 60S ribosomal protein L4 vs. Ensembl Nematostella
Match: EDO31005 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SZ61])

HSP 1 Score: 362.844 bits (930), Expect = 5.498e-124
Identity = 197/346 (56.94%), Postives = 251/346 (72.54%), Query Frame = 2
Query:  116 SRPLINVYKVNLPTDDSKPKLECIIDGSVKFPSVFNAPLRMDILSSVHDLIRKNSRQPYAVSTGAGHNNSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGHMFAPTKVWRKWHHRVPIQQRRYAMVTAIAATGYTPLVAARGHKVEKVPELPLVISEEYEGLKKTKMAVKVLKGCGVWPDIEKVKDSRKVRAGRGKMRNRRYVQKCGPIVIYKSDNGITKAFRNIPGVTXXXXXXXXXXXXSPGGHMGRLTIWTKPAIEELEKIYGSWKTASQSKVDFLLPAPTMKNADLMRIINSEEIQSVLKARNMXXXXXXXXXXXXXXXNDGVMAKLNPYAAGEKK 1153
            +RP+I V+  N  +            G    P+VF AP+R D+++ VH  I KN RQPYAV+  AGH  SAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGG MFAPTK WRKWH +V +QQRR+A+ +A+AA+    L+ ARGH++EK+ E+PLVIS+  E + KT  AVK+LK    + D+EK  DS+K+RAG+GKMRNRR V + GP++IY +D G+ +AFRN+PGV L +V  LN+LKL PGGH+GR  IW++ A E+L+ IYG+WK AS  K +F LP P M N+DL R+INSEEIQSV+  R MK + + +  KKNPLKN G M +LNPYA   K+
Sbjct:    3 ARPVITVFNENGESA-----------GQTTLPAVFKAPIRPDLVNFVHSNIAKNKRQPYAVNKLAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKTWRKWHTKVNVQQRRFAVCSALAASALPALIMARGHRIEKIAEVPLVISDAIESVTKTSAAVKLLKAVNAYEDVEKCIDSKKIRAGKGKMRNRRTVMRKGPLIIYNNDQGLRQAFRNLPGVELQHVDRLNLLKLCPGGHLGRFIIWSQGAFEKLDAIYGTWKKASTEKANFNLPRPIMLNSDLTRLINSEEIQSVI--RPMKPKARRAIHKKNPLKNLGTMLRLNPYAKSAKR 335          
BLAST of 60S ribosomal protein L4 vs. Ensembl Medaka
Match: rpl4 (ribosomal protein L4 [Source:NCBI gene;Acc:101162922])

HSP 1 Score: 362.844 bits (930), Expect = 1.630e-123
Identity = 192/343 (55.98%), Postives = 249/343 (72.59%), Query Frame = 2
Query:  110 AMSRPLINVYKVNLPTDDSKPKLECIIDGSVKFPSVFNAPLRMDILSSVHDLIRKNSRQPYAVSTGAGHNNSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGHMFAPTKVWRKWHHRVPIQQRRYAMVTAIAATGYTPLVAARGHKVEKVPELPLVISEEYEGLKKTKMAVKVLKGCGVWPDIEKVKDSRKVRAGRGKMRNRRYVQKCGPIVIYKSDNGITKAFRNIPGVTXXXXXXXXXXXXSPGGHMGRLTIWTKPAIEELEKIYGSWKTASQSKVDFLLPAPTMKNADLMRIINSEEIQSVLKARNMXXXXXXXXXXXXXXXNDGVMAKLNPYA 1138
            A +RPLI+VY     +          +  +V  P+VF AP+R D+++ VH  +RKN+RQPYAVS  AGH  SAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGG MFAPTK WR+WH R+   Q+RYA+ +A+AA+    LV ++GH++E++PE+PLV+ ++ EG KKTK AV +LK    W DI+KV  S+++RAG+GKMRNRR +Q+ GP +IY  D G+TKAFRNIPG+TL NV  LN+L+L+PGGH+GR  IWT+ A  +L+ +YG+W+  +  KVD+ LP   M N DL RI+ SEEIQ  L+A N   +     +KKNPLKN  +M KLNPYA
Sbjct:    2 ACARPLISVYSEKGES----------VGKNVLMPAVFKAPIRPDVVNFVHTNMRKNNRQPYAVSELAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKTWRRWHRRINTPQKRYAICSALAASAIPALVMSKGHRIEEIPEVPLVVEDKVEGYKKTKEAVLLLKKLKAWNDIKKVYASQRMRAGKGKMRNRRRIQRKGPCIIYNQDAGLTKAFRNIPGITLQNVNKLNLLRLAPGGHVGRFCIWTESAFRKLDDLYGTWRKPATLKVDYNLPMHKMTNTDLSRILKSEEIQKALRAPN--KKINRRVMKKNPLKNLKIMFKLNPYA 332          
BLAST of 60S ribosomal protein L4 vs. Planmine SMEST
Match: SMESG000009479.1 (SMESG000009479.1)

HSP 1 Score: 688.723 bits (1776), Expect = 0.000e+0
Identity = 364/366 (99.45%), Postives = 365/366 (99.73%), Query Frame = 2
Query:  113 MSRPLINVYKVNLPTDDSKPKLECIIDGSVKFPSVFNAPLRMDILSSVHDLIRKNSRQPYAVSTGAGHNNSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGHMFAPTKVWRKWHHRVPIQQRRYAMVTAIAATGYTPLVAARGHKVEKVPELPLVISEEYEGLKKTKMAVKVLKGCGVWPDIEKVKDSRKVRAGRGKMRNRRYVQKCGPIVIYKSDNGITKAFRNIPGVTXXXXXXXXXXXXSPGGHMGRLTIWTKPAIEELEKIYGSWKTASQSKVDFLLPAPTMKNADLMRIINSEEIQSVLKARNMXXXXXXXXXXXXXXXNDGVMAKLNPYAAGEKKINKRKSCGIDNVLPKKIKA 1210
            MSRPLINVYKVNLPTDDSKPKLECIIDGSVKFPSVFNAPLRMDILSSVHDLIRKNSRQPYAVSTGAGHNNSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGHMFAPTKVWRKWHHRVPIQQRRYAMVTAIAATGYTPLVAARGHKVEKVPELPLVISEEYEGLKKTKMAVKVLKGCGVW DIEKVKDSRKVRAGRGKMRNRRYVQKCGPIVIYKSDNGITKAFRNIPGVTLINVKNLNILKLSPGGHMGRLTIWTKPAIEELEKIYGSWKTASQSKVDF+LPAPTMKNADLMRIINSEEIQSVLKARNMKAQKKSSKVKKNPLKNDGVMAKLNPYAAGEKKINKRKSCGIDNVLPKKIKA
Sbjct:    1 MSRPLINVYKVNLPTDDSKPKLECIIDGSVKFPSVFNAPLRMDILSSVHDLIRKNSRQPYAVSTGAGHNNSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGHMFAPTKVWRKWHHRVPIQQRRYAMVTAIAATGYTPLVAARGHKVEKVPELPLVISEEYEGLKKTKMAVKVLKGCGVWADIEKVKDSRKVRAGRGKMRNRRYVQKCGPIVIYKSDNGITKAFRNIPGVTLINVKNLNILKLSPGGHMGRLTIWTKPAIEELEKIYGSWKTASQSKVDFVLPAPTMKNADLMRIINSEEIQSVLKARNMKAQKKSSKVKKNPLKNDGVMAKLNPYAAGEKKINKRKSCGIDNVLPKKIKA 366          
The following BLAST results are available for this feature:
BLAST of 60S ribosomal protein L4 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4
Match NameE-valueIdentityDescription
RPL42.308e-11257.14ribosomal protein L4 [Source:HGNC Symbol;Acc:HGNC:... [more]
RPL42.745e-6755.42ribosomal protein L4 [Source:HGNC Symbol;Acc:HGNC:... [more]
RPL42.976e-5855.21ribosomal protein L4 [Source:HGNC Symbol;Acc:HGNC:... [more]
RPL45.807e-4857.96ribosomal protein L4 [Source:HGNC Symbol;Acc:HGNC:... [more]
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BLAST of 60S ribosomal protein L4 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rpl-41.497e-11050.0060S ribosomal protein L4 [Source:UniProtKB/Swiss-... [more]
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BLAST of 60S ribosomal protein L4 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
RpL46.484e-11453.67gene:FBgn0003279 transcript:FBtr0085248[more]
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BLAST of 60S ribosomal protein L4 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rpl41.330e-12557.14ribosomal protein L4 [Source:ZFIN;Acc:ZDB-GENE-030... [more]
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BLAST of 60S ribosomal protein L4 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rpl46.067e-11455.94ribosomal protein L4 [Source:NCBI gene;Acc:1003272... [more]
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BLAST of 60S ribosomal protein L4 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Rpl43.101e-11357.14ribosomal protein L4 [Source:MGI Symbol;Acc:MGI:19... [more]
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BLAST of 60S ribosomal protein L4 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P49165|RL4_URECA1.080e-12457.0660S ribosomal protein L4 OS=Urechis caupo OX=6431 ... [more]
sp|Q9SF40|RL4A_ARATH6.620e-12055.8160S ribosomal protein L4-1 OS=Arabidopsis thaliana... [more]
sp|P49691|RL4B_ARATH3.978e-11955.2160S ribosomal protein L4-2 OS=Arabidopsis thaliana... [more]
sp|P35679|RL4A_SCHPO6.910e-11952.7460S ribosomal protein L4-A OS=Schizosaccharomyces ... [more]
sp|Q9P784|RL4B_SCHPO8.496e-11951.8260S ribosomal protein L4-B OS=Schizosaccharomyces ... [more]
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BLAST of 60S ribosomal protein L4 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A504YWQ01.454e-14465.13Ribosomal protein l4 OS=Fasciola gigantica OX=4683... [more]
A0A5J4P0I81.722e-14463.97Large subunit ribosomal protein L4e OS=Paragonimus... [more]
A0A4E0RYD32.084e-14465.13Ribosomal protein l4 OS=Fasciola hepatica OX=6192 ... [more]
A0A1S8WLA02.114e-14263.43Ribosomal protein, L4/L1 family OS=Opisthorchis vi... [more]
A0A4S2LI532.147e-14163.32Ribos_L4_asso_C domain-containing protein OS=Opist... [more]
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BLAST of 60S ribosomal protein L4 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rpl42.220e-11955.98ribosomal protein L4 [Source:NCBI gene;Acc:1030283... [more]
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BLAST of 60S ribosomal protein L4 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rpl41.093e-11356.65ribosomal protein L4 [Source:ZFIN;Acc:ZDB-GENE-030... [more]
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BLAST of 60S ribosomal protein L4 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2
Match NameE-valueIdentityDescription
RPL4B4.240e-9753.64Ribosomal 60S subunit protein L4B; homologous to m... [more]
RPL4A4.624e-9753.64Ribosomal 60S subunit protein L4A; N-terminally ac... [more]
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BLAST of 60S ribosomal protein L4 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO310055.498e-12456.94Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of 60S ribosomal protein L4 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rpl41.630e-12355.98ribosomal protein L4 [Source:NCBI gene;Acc:1011629... [more]
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BLAST of 60S ribosomal protein L4 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000009479.10.000e+099.45SMESG000009479.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30000134 ID=SMED30000134|Name=60S ribosomal protein L4|organism=Schmidtea mediterranea sexual|type=transcript|length=1238bp
TTTAATTATTAATAAAATTTCGTGATATTTACAAAACTTTTAAATTTTAT
TTTACTGATTAAAATTTGTTTTAATTAATTGAACATATTGAATAAAAAAA
AATTCAAAAGCAATGTCTCGTCCATTGATAAATGTTTATAAAGTTAATTT
ACCCACTGATGATTCCAAGCCTAAGCTTGAATGCATTATTGATGGATCAG
TTAAATTTCCATCAGTCTTTAATGCTCCTTTGAGAATGGATATTTTGTCA
TCAGTACATGATCTAATTAGGAAAAATAGCCGTCAACCATATGCTGTTAG
TACTGGTGCTGGTCATAATAATTCGGCTGAATCTTGGGGAACTGGTAGAG
CTGTTGCTCGTATCCCAAGAGTTCGTGGAGGTGGAACTCACAGATCAGGT
CAAGGTGCCTTTGGAAACATGTGCCGTGGAGGTCATATGTTCGCCCCAAC
TAAAGTTTGGAGAAAATGGCATCATCGTGTCCCAATTCAACAAAGAAGAT
ATGCTATGGTAACTGCAATTGCTGCAACCGGATACACCCCACTTGTTGCT
GCTAGAGGACATAAAGTTGAAAAAGTCCCTGAACTTCCATTGGTTATCAG
TGAAGAATATGAAGGTTTGAAGAAAACTAAAATGGCTGTTAAGGTCTTGA
AAGGATGCGGTGTCTGGCCAGATATTGAAAAGGTAAAAGATTCGAGGAAA
GTGCGAGCTGGAAGAGGAAAAATGCGCAATAGAAGATATGTTCAAAAATG
TGGTCCAATTGTTATATACAAATCAGACAATGGTATCACTAAAGCATTCA
GAAATATTCCAGGTGTTACACTCATTAACGTTAAGAACTTAAACATTCTT
AAATTGTCTCCTGGTGGTCATATGGGAAGATTGACTATTTGGACAAAACC
AGCAATTGAAGAATTGGAAAAGATTTATGGATCATGGAAAACAGCAAGTC
AATCTAAAGTTGACTTTCTATTACCAGCGCCGACAATGAAAAATGCTGAT
TTAATGAGAATTATTAACTCTGAAGAAATCCAGTCAGTTTTAAAAGCAAG
AAATATGAAAGCTCAAAAGAAATCTAGTAAGGTTAAAAAGAATCCATTGA
AGAATGATGGAGTTATGGCTAAATTGAATCCATATGCAGCGGGTGAAAAG
AAGATTAACAAAAGAAAATCATGTGGAATTGACAATGTTTTGCCCAAAAA
AATCAAAGCATAATTTTTAATGAATAAATTTGGATTTT
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protein sequence of SMED30000134-orf-1

>SMED30000134-orf-1 ID=SMED30000134-orf-1|Name=SMED30000134-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=367bp
MSRPLINVYKVNLPTDDSKPKLECIIDGSVKFPSVFNAPLRMDILSSVHD
LIRKNSRQPYAVSTGAGHNNSAESWGTGRAVARIPRVRGGGTHRSGQGAF
GNMCRGGHMFAPTKVWRKWHHRVPIQQRRYAMVTAIAATGYTPLVAARGH
KVEKVPELPLVISEEYEGLKKTKMAVKVLKGCGVWPDIEKVKDSRKVRAG
RGKMRNRRYVQKCGPIVIYKSDNGITKAFRNIPGVTLINVKNLNILKLSP
GGHMGRLTIWTKPAIEELEKIYGSWKTASQSKVDFLLPAPTMKNADLMRI
INSEEIQSVLKARNMKAQKKSSKVKKNPLKNDGVMAKLNPYAAGEKKINK
RKSCGIDNVLPKKIKA*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000120progenitor cell
PLANA:0000429neoblast
PLANA:0002032epidermal cell
PLANA:0002109X1 cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR023574Ribosomal_L4_dom_sf
IPR025755Ribos_L4_C_dom
IPR002136Ribosomal_L4/L1e
Vocabulary: molecular function
TermDefinition
GO:0003735structural constituent of ribosome
Vocabulary: biological process
TermDefinition
GO:0006412translation
Vocabulary: cellular component
TermDefinition
GO:0005840ribosome
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002136Ribosomal protein L4/L1ePFAMPF00573Ribosomal_L4coord: 33..270
e-value: 2.0E-37
score: 128.7
IPR023574Ribosomal protein L4 domain superfamilyGENE3DG3DSA:3.40.1370.10coord: 1..312
e-value: 1.1E-124
score: 417.3
IPR023574Ribosomal protein L4 domain superfamilySUPERFAMILYSSF52166Ribosomal protein L4coord: 28..270
IPR02575560S ribosomal protein L4, C-terminal domainPFAMPF14374Ribos_L4_asso_Ccoord: 284..350
e-value: 2.0E-24
score: 85.4
NoneNo IPR availablePANTHERPTHR1943160S RIBOSOMAL PROTEIN L4coord: 2..347