Ribosome-binding protein 1
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30000038 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 20
Homology
BLAST of Ribosome-binding protein 1 vs. TrEMBL
Match: A0A1I8GEB2 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 88.5817 bits (218), Expect = 3.425e-14 Identity = 52/114 (45.61%), Postives = 75/114 (65.79%), Query Frame = 1 Query: 1756 YKTGLESTESILSKLQTSVNEEESKWRESLIXXXXXXXXXXXXXXIVYNDLMRIRNSRTDN-NELNFAYHCVDKSLSSLLDEMEARMIHFEKSLETVVQERDTALSQISSQING 2094 YK LE +E +L KL+ SV EEE++W+ +L SE +VEK K L ++L R R S D+ +LNFAYHCV+KSL++++DEME ++ E L V ERD+A QI +++ G Sbjct: 454 YKLALEYSEGVLKKLEASVEEEEARWKSALCASEADVEKYKAALASAVSELRRYRGSSNDDLTDLNFAYHCVEKSLTNIVDEMEQKVKEMEDKLGKVSAERDSA-KQIQAKLQG 566
BLAST of Ribosome-binding protein 1 vs. TrEMBL
Match: A0A267H9F3 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig001185g1 PE=4 SV=1) HSP 1 Score: 87.4261 bits (215), Expect = 5.164e-14 Identity = 52/115 (45.22%), Postives = 76/115 (66.09%), Query Frame = 1 Query: 1753 QYKTGLESTESILSKLQTSVNEEESKWRESLIXXXXXXXXXXXXXXIVYNDLMRIRNSRTDN-NELNFAYHCVDKSLSSLLDEMEARMIHFEKSLETVVQERDTALSQISSQING 2094 +YK LE +E +L KL+ SV EEE++W+ +L SE +VEK K L ++L R R S D+ +LNFAYHCV+KSL++++DEME ++ E L V ERD+A QI +++ G Sbjct: 258 KYKLALEYSEGVLKKLEASVEEEEARWKSALCASEADVEKYKAALASAVSELRRYRGSSNDDLTDLNFAYHCVEKSLTNIVDEMEQKVKEMEDKLGKVSAERDSA-KQIQAKLQG 371
BLAST of Ribosome-binding protein 1 vs. TrEMBL
Match: A0A1I8HIP6 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 75.8702 bits (185), Expect = 3.038e-10 Identity = 41/87 (47.13%), Postives = 62/87 (71.26%), Query Frame = 1 Query: 1756 YKTGLESTESILSKLQTSVNEEESKWRESLIXXXXXXXXXXXXXXIVYNDLMRIRNSRTDN-NELNFAYHCVDKSLSSLLDEMEARM 2013 YK LE +E +L KL+ SV EEE++W+ +L SE +VEK K L ++L R R S D+ +LNFAYHCV+KSL++++DE++A++ Sbjct: 402 YKLALEYSEGVLKKLEASVEEEEARWKSALCASEADVEKYKAALASAVSELRRYRGSSNDDLTDLNFAYHCVEKSLTNIVDEIQAKL 488
BLAST of Ribosome-binding protein 1 vs. TrEMBL
Match: A0A0B7BL77 (Uncharacterized protein OS=Arion vulgaris OX=1028688 GN=ORF192003 PE=4 SV=1) HSP 1 Score: 73.9442 bits (180), Expect = 1.308e-9 Identity = 99/355 (27.89%), Postives = 180/355 (50.70%), Query Frame = 1 Query: 1300 DLLTAKSAH-TDLLEENLRLQKQCAELEQSTVLKQQDLADFDNKLKQNEEQITCLKKQI------NDLNT-MNINLSVLKSDLEKQVDQLHSSQSTVNSQEIIELTE-KVKILTQQLIDAKTELATRQMKL------EEFDSSCAQHLTQLKELTTERDNLLALVDQYKTGLESTESILSKLQTSVNEEESKWRESLIXXXXXXXXXXXXXXIVYNDLMRIRNSRTDNNELNFAYHCVDKSLSSLLDEMEARMIHFEKSLE-------TVVQERDTALSQIS-SQINGVSVNHEIVNGDDHSIQNDDSEKLIVE----LEHQTELNKSLENQCNKFKSLIDIGKDALESEKSRVR 2283 ++LTAK +L E+N R+ KQ + E+ V +L + KLKQ E + LK + N+ T + + S LKSDLE N I+ TE K++ L ++ IDA+ + ++K E+ DS Q + +L+ L ++D + V +Y+ L TE L + ++ + +E W+E L +++++ ++K + ++ + S+ D ++L FAYHCV+KSL++++DEM+ ++ E L + +E++T ++Q++ +QI ++ +GD S+ EK +VE LE + + NK L K +I G+DAL E++ V+ Sbjct: 418 NVLTAKGNKLIELEEQNKRIHKQLNDYEKQFV----ELNSQNEKLKQVEAENYQLKSSVTSKGGDNERYTQLETDNSRLKSDLE-------------NYHSIVAETENKLRQL-EKSIDAEEKKWQEKLKQAQSHPKEQGDSGLPQRIKELELLVAQQD---SQVQEYRRVLSLTEDRLREFESKIESQEKTWQEKLETAQSKLTQTKTPVSSSSQEIQVSQGSQEDLSDLGFAYHCVEKSLTTIVDEMQTKVAELEDELREAHEMIIVITKEKETVITQLTETQI-------QVSSGDTKSL-----EKELVEIRTILESERKKNKDLSLNVVKLNGIIKTGQDALSQEQNVVK 739
BLAST of Ribosome-binding protein 1 vs. TrEMBL
Match: A0A0B7BLU2 (Uncharacterized protein OS=Arion vulgaris OX=1028688 GN=ORF192034 PE=4 SV=1) HSP 1 Score: 73.9442 bits (180), Expect = 1.386e-9 Identity = 99/355 (27.89%), Postives = 180/355 (50.70%), Query Frame = 1 Query: 1300 DLLTAKSAH-TDLLEENLRLQKQCAELEQSTVLKQQDLADFDNKLKQNEEQITCLKKQI------NDLNT-MNINLSVLKSDLEKQVDQLHSSQSTVNSQEIIELTE-KVKILTQQLIDAKTELATRQMKL------EEFDSSCAQHLTQLKELTTERDNLLALVDQYKTGLESTESILSKLQTSVNEEESKWRESLIXXXXXXXXXXXXXXIVYNDLMRIRNSRTDNNELNFAYHCVDKSLSSLLDEMEARMIHFEKSLE-------TVVQERDTALSQIS-SQINGVSVNHEIVNGDDHSIQNDDSEKLIVE----LEHQTELNKSLENQCNKFKSLIDIGKDALESEKSRVR 2283 ++LTAK +L E+N R+ KQ + E+ V +L + KLKQ E + LK + N+ T + + S LKSDLE N I+ TE K++ L ++ IDA+ + ++K E+ DS Q + +L+ L ++D + V +Y+ L TE L + ++ + +E W+E L +++++ ++K + ++ + S+ D ++L FAYHCV+KSL++++DEM+ ++ E L + +E++T ++Q++ +QI ++ +GD S+ EK +VE LE + + NK L K +I G+DAL E++ V+ Sbjct: 418 NVLTAKGNKLIELEEQNKRIHKQLNDYEKQFV----ELNSQNEKLKQVEAENYQLKSSVTSKGGDNERYTQLETDNSRLKSDLE-------------NYHSIVAETENKLRQL-EKSIDAEEKKWQEKLKQAQSHPKEQGDSGLPQRIKELELLVAQQD---SQVQEYRRVLSLTEDRLREFESKIESQEKTWQEKLETAQSKLTQTKTPVSSSSQEIQVSQGSQEDLSDLGFAYHCVEKSLTTIVDEMQTKVAELEDELREAHEMIIVITKEKETVITQLTETQI-------QVSSGDTKSL-----EKELVEIRTILESERKKNKDLSLNVVKLNGIIKTGQDALSQEQNVVK 739
BLAST of Ribosome-binding protein 1 vs. Planmine SMEST
Match: SMESG000031975.1 (SMESG000031975.1) HSP 1 Score: 1400.96 bits (3625), Expect = 0.000e+0 Identity = 764/768 (99.48%), Postives = 765/768 (99.61%), Query Frame = 1 Query: 61 MISSFLELCLTFGLGILISGLAFLWTRRSLSVDDDIVLPKEDYKIKNKISKVPVKAKVTKKDFTTAKLKNNIKTAQKSTVNAVKNNETFKDVTENKVSAVERNRNDSTAEWXXXXXXXXXXXXESKSDSFDLSAQEESVKHSTLENNQKETVKSCDKPKVENPIKIELDKELKTEKVPEKPIEAVKNQTVKSKKFVTVEEEPKTLPQPEKIETKPTPVSPDSDNIQQKIAEMMVDLDKMRNNQKDDNKGNSQIXXXXXXXXXXXXTSQANEQKLLEDVKKKGQAVSDLKNQFLIIQSELTKNIVANQSINREKLSLENRLRSVEEQNALITEKCQQFENLKIENEGLEKNFKAIEQELKSVKLEYSDIIKSQEREMLTQINVLQSEKHRMQDQLVRVQNDLIGNNNKMQNVQQDLLTAKSAHTDLLEENLRLQKQCAELEQSTVLKQQDLADFDNKLKQNEEQITCLKKQINDLNTMNINLSVLKSDLEKQVDQLHSSQSTVNSQEIIELTEKVKILTQQLIDAKTELATRQMKLEEFDSSCAQHLTQLKELTTERDNLLALVDQYKTGLESTESILSKLQTSVNEEESKWRESLIXXXXXXXXXXXXXXIVYNDLMRIRNSRTDNNELNFAYHCVDKSLSSLLDEMEARMIHFEKSLETVVQERDTALSQISSQINGVSVNHEIVNGDDHSIQNDDSEKLIVELEHQTELNKSLENQCNKFKSLIDIGKDALESEKSRVRXXXXXXXXXXXXITNVSTNNVEVSLDA 2364 MISSFLELCLTFGLGILISGLAFLWTRRSLSVDDDIVLPKEDYKIKNKISKVPVKAKVTKKDFTTAKLKNNIKTAQKSTVNAVKNNETFKDVTENKVSAVERNRNDSTAEWTKVTKPTKKPKKESKSDSFDLSAQEESVKHSTLENNQKETVKSCDKPKVENPIKIELDKELKTEKVPEKPIE VKNQTVKSKKFVTVEEEPKTLPQPEKIETKPT VS DSDNIQQKIAEMMVDLDKMRNNQKDDNKGNSQISKELESLKLKLKTSQANEQKLLEDVKKKGQAVSDLKNQFLIIQSELTKNIVANQSINREKLSLENRLRSVEEQNALITEKCQQFENLKIENEGLEKNFKAIEQELKSVKLEYSDIIKSQEREMLTQINVLQSEKHRMQDQLVRVQNDLIGNNNKMQNVQQDLLTAKSAHTDLLEENLRLQKQCAELEQSTVLKQQDLADFDNKLKQNEEQITCLKKQINDLNTMNINLSVLKSDLEKQVDQLHSSQSTVNSQEIIELTEKVKILTQQLIDAKTELATRQMKLEEFDSSCAQHLTQLKELTTERDNLLALVDQYKTGLESTESILSKLQTSVNEEESKWRESLIKSENEVEKSKKELEIVYNDLMRIRNSRTDNNELNFAYHCVDKSLSSLLDEMEARMIHFEKSLETVVQERDTALSQISSQINGVSVNHEIVNG+DHSIQNDDSEKLIVELEHQTELNKSLENQCNKFKSLIDIGKDALESEKSRVRELEQELSSLQSLITNVSTNNVEVSLDA Sbjct: 1 MISSFLELCLTFGLGILISGLAFLWTRRSLSVDDDIVLPKEDYKIKNKISKVPVKAKVTKKDFTTAKLKNNIKTAQKSTVNAVKNNETFKDVTENKVSAVERNRNDSTAEWTKVTKPTKKPKKESKSDSFDLSAQEESVKHSTLENNQKETVKSCDKPKVENPIKIELDKELKTEKVPEKPIEPVKNQTVKSKKFVTVEEEPKTLPQPEKIETKPTLVSHDSDNIQQKIAEMMVDLDKMRNNQKDDNKGNSQISKELESLKLKLKTSQANEQKLLEDVKKKGQAVSDLKNQFLIIQSELTKNIVANQSINREKLSLENRLRSVEEQNALITEKCQQFENLKIENEGLEKNFKAIEQELKSVKLEYSDIIKSQEREMLTQINVLQSEKHRMQDQLVRVQNDLIGNNNKMQNVQQDLLTAKSAHTDLLEENLRLQKQCAELEQSTVLKQQDLADFDNKLKQNEEQITCLKKQINDLNTMNINLSVLKSDLEKQVDQLHSSQSTVNSQEIIELTEKVKILTQQLIDAKTELATRQMKLEEFDSSCAQHLTQLKELTTERDNLLALVDQYKTGLESTESILSKLQTSVNEEESKWRESLIKSENEVEKSKKELEIVYNDLMRIRNSRTDNNELNFAYHCVDKSLSSLLDEMEARMIHFEKSLETVVQERDTALSQISSQINGVSVNHEIVNGEDHSIQNDDSEKLIVELEHQTELNKSLENQCNKFKSLIDIGKDALESEKSRVRELEQELSSLQSLITNVSTNNVEVSLDA 768 The following BLAST results are available for this feature:
BLAST of Ribosome-binding protein 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of Ribosome-binding protein 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of Ribosome-binding protein 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Ribosome-binding protein 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of Ribosome-binding protein 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of Ribosome-binding protein 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of Ribosome-binding protein 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of Ribosome-binding protein 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Ribosome-binding protein 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of Ribosome-binding protein 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Ribosome-binding protein 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Ribosome-binding protein 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of Ribosome-binding protein 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of Ribosome-binding protein 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30000038 ID=SMED30000038|Name=Ribosome-binding protein 1|organism=Schmidtea mediterranea sexual|type=transcript|length=2845bpback to top protein sequence of SMED30000038-orf-1 >SMED30000038-orf-1 ID=SMED30000038-orf-1|Name=SMED30000038-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=769bp MISSFLELCLTFGLGILISGLAFLWTRRSLSVDDDIVLPKEDYKIKNKISback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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