SMED30000022

Overview
NameSMED30000022
Smed IDSMED30000022
Length (bp)971
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30000022 (SMED30000022) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30000022 (SMED30000022) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30000022 (SMED30000022) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30000022 (SMED30000022) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30000022

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30000022SMESG000061316.1 SmedASXL_006851SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
parenchymaSMED30000022SMESG000061316.1 dd_Smed_v4_385_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30000022SMESG000061316.1 dd_385dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult fluorescence in situ hybridization evidence
parapharyngeal regionSMED30000022SMESG000061316.1 dd_Smed_v6_385_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
head regionSMED30000022SMESG000061316.1 OX_Smed_1.0.00608ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30000022 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.003279:14985..18192 +4829
v31.003279:13232..14200 +4515
v31.003279:21103..21942 -2716
Homology
BLAST of SMED30000022 vs. Planmine SMEST
Match: SMESG000061316.1 (SMESG000061316.1)

HSP 1 Score: 536.954 bits (1382), Expect = 9.248e-175
Identity = 323/323 (100.00%), Postives = 323/323 (100.00%), Query Frame = 3
Query:    3 HHPKISGKIVVRGRKSRNPHHRFHLDTRKQVGFLKLGVERLFLGFRDKDDTENILKTIKIHAPHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNIKLSLGAQLGGKRGFFGLNEXXXXXXXXXXXKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVXXXXXXXXXXXXXXXXXXXIDTKHRQHSVKISGKVKTHGKIHHPXXXXXXXXXXXXXXXXHKKPT 971
            HHPKISGKIVVRGRKSRNPHHRFHLDTRKQVGFLKLGVERLFLGFRDKDDTENILKTIKIHAPHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNIKLSLGAQLGGKRGFFGLNERLSSLLPRLRLKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVKIKIKQKKINKKYLKIVGKIDTKHRQHSVKISGKVKTHGKIHHPKVGGKIVIKSGKVDIKHKKPT
Sbjct: 2005 HHPKISGKIVVRGRKSRNPHHRFHLDTRKQVGFLKLGVERLFLGFRDKDDTENILKTIKIHAPHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNIKLSLGAQLGGKRGFFGLNERLSSLLPRLRLKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVKIKIKQKKINKKYLKIVGKIDTKHRQHSVKISGKVKTHGKIHHPKVGGKIVIKSGKVDIKHKKPT 2327          

HSP 2 Score: 531.561 bits (1368), Expect = 6.563e-173
Identity = 301/302 (99.67%), Postives = 302/302 (100.00%), Query Frame = 3
Query:    3 HHPKISGKIVVRGRKSRNPHHRFHLDTRKQVGFLKLGVERLFLGFRDKDDTENILKTIKIHAPHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNIKLSLGAQLGGKRGFFGLNEXXXXXXXXXXXKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVXXXXXXXXXXXXXXXXXXXIDTKHRQHSVKISGKVKTHGKIHHP 908
            HHPKISGKIVVRGRKSRNPHHRFHLDTRKQVGFLKLGVERLFLGFRDKDDTENILKTIKIHAPHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNIKLSLGAQLGGKRGFFGLNERLSSLLPRLRLKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVKIKIKQKKINKKYLKIVGKIDTKHRQHSVKI+GKVKTHGKIHHP
Sbjct:  847 HHPKISGKIVVRGRKSRNPHHRFHLDTRKQVGFLKLGVERLFLGFRDKDDTENILKTIKIHAPHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNIKLSLGAQLGGKRGFFGLNERLSSLLPRLRLKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVKIKIKQKKINKKYLKIVGKIDTKHRQHSVKITGKVKTHGKIHHP 1148          

HSP 3 Score: 531.561 bits (1368), Expect = 6.563e-173
Identity = 301/302 (99.67%), Postives = 302/302 (100.00%), Query Frame = 3
Query:    3 HHPKISGKIVVRGRKSRNPHHRFHLDTRKQVGFLKLGVERLFLGFRDKDDTENILKTIKIHAPHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNIKLSLGAQLGGKRGFFGLNEXXXXXXXXXXXKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVXXXXXXXXXXXXXXXXXXXIDTKHRQHSVKISGKVKTHGKIHHP 908
            HHPKISGKIVVRGRKSRNPHHRFHLDTRKQVGFLKLGVERLFLGFRDKDDTENILKTIKIHAPHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNIKLSLGAQLGGKRGFFGLNERLSSLLPRLRLKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVKIKIKQKKINKKYLKIVGKIDTKHRQHSVKI+GKVKTHGKIHHP
Sbjct: 1407 HHPKISGKIVVRGRKSRNPHHRFHLDTRKQVGFLKLGVERLFLGFRDKDDTENILKTIKIHAPHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNIKLSLGAQLGGKRGFFGLNERLSSLLPRLRLKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVKIKIKQKKINKKYLKIVGKIDTKHRQHSVKITGKVKTHGKIHHP 1708          

HSP 4 Score: 531.561 bits (1368), Expect = 6.563e-173
Identity = 301/302 (99.67%), Postives = 302/302 (100.00%), Query Frame = 3
Query:    3 HHPKISGKIVVRGRKSRNPHHRFHLDTRKQVGFLKLGVERLFLGFRDKDDTENILKTIKIHAPHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNIKLSLGAQLGGKRGFFGLNEXXXXXXXXXXXKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVXXXXXXXXXXXXXXXXXXXIDTKHRQHSVKISGKVKTHGKIHHP 908
            HHPKISGKIVVRGRKSRNPHHRFHLDTRKQVGFLKLGVERLFLGFRDKDDTENILKTIKIHAPHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNIKLSLGAQLGGKRGFFGLNERLSSLLPRLRLKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVKIKIKQKKINKKYLKIVGKIDTKHRQHSVKI+GKVKTHGKIHHP
Sbjct: 1706 HHPKISGKIVVRGRKSRNPHHRFHLDTRKQVGFLKLGVERLFLGFRDKDDTENILKTIKIHAPHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNIKLSLGAQLGGKRGFFGLNERLSSLLPRLRLKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVKIKIKQKKINKKYLKIVGKIDTKHRQHSVKITGKVKTHGKIHHP 2007          

HSP 5 Score: 480.33 bits (1235), Expect = 8.584e-155
Identity = 276/281 (98.22%), Postives = 281/281 (100.00%), Query Frame = 3
Query:   66 RFHLDTRKQVGFLKLGVERLFLGFRDKDDTENILKTIKIHAPHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNIKLSLGAQLGGKRGFFGLNEXXXXXXXXXXXKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVXXXXXXXXXXXXXXXXXXXIDTKHRQHSVKISGKVKTHGKIHHP 908
            ++++DTRKQVGFLKLGVERLFLGFRDKDDTENILKTIKIHAPHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNIKLSLGAQLGGKRGFFGLNERLSSLLPRLRLKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVKIKIKQKKINKKYLKIVGKIDTKHRQHSVKI+GKVKTHGKIHHP
Sbjct:  569 KYNIDTRKQVGFLKLGVERLFLGFRDKDDTENILKTIKIHAPHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNIKLSLGAQLGGKRGFFGLNERLSSLLPRLRLKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVKIKIKQKKINKKYLKIVGKIDTKHRQHSVKITGKVKTHGKIHHP 849          

HSP 6 Score: 436.802 bits (1122), Expect = 1.473e-139
Identity = 263/302 (87.09%), Postives = 264/302 (87.42%), Query Frame = 3
Query:    3 HHPKISGKIVVRGRKSRNPHHRFHLDTRKQVGFLKLGVERLFLGFRDKDDTENILKTIKIHAPHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNIKLSLGAQLGGKRGFFGLNEXXXXXXXXXXXKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVXXXXXXXXXXXXXXXXXXXIDTKHRQHSVKISGKVKTHGKIHHP 908
            HHPKISGKIVVRGRKSRNPHHRFHLDTRKQ                                      EIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNIKLSLGAQLGGKRGFFGLNERLSSLLPRLRLKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVKIKIKQKKINKKYLKIVGKIDTKHRQHSVKI+GKVKTHGKIHHP
Sbjct: 1146 HHPKISGKIVVRGRKSRNPHHRFHLDTRKQ--------------------------------------EIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNIKLSLGAQLGGKRGFFGLNERLSSLLPRLRLKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVKIKIKQKKINKKYLKIVGKIDTKHRQHSVKITGKVKTHGKIHHP 1409          

HSP 7 Score: 111.694 bits (278), Expect = 6.975e-27
Identity = 79/223 (35.43%), Postives = 126/223 (56.50%), Query Frame = 3
Query:   96 GFLKLGVERLFLGFRDKDDTENILKTIKIHAPHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNIKLSLGAQLGGKRGFFGLNEXXXXXXXXXXXKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVI 764
            GFL+LG+ +++         EN +K +K     ++I ++Q L  E+  L + L   E +  +L  I +LLY+L+ NNIKFE LR KYF  LN+  +F  +   K +V     K  L  QLGG  GFFGL + L  L+  L+   +  NVER++KP V   +W  ++ L  E+++I +KL  +E+   K   +S +LFKL  K   +E Y++K+F K+ ++ +I
Sbjct:  326 GFLELGLFKVYTLSSFDRVIENYIKMMKSKVSQSIIIKVQNLFREIEYLFKGLNSREKNKRKLEYIIHLLYKLNDNNIKFERLRKKYFRELNMIDVFQKNMKKKNKVSK---KRKLINQLGGNEGFFGLQQELGELIANLKYHQLEGNVERYIKPKVPRNLWYSLKKLIKENIEILKKLNRLEKQHRKFTILSKVLFKLKTKIKTYETYKVKHFRKIPIRKII 545          

HSP 8 Score: 83.1889 bits (204), Expect = 2.822e-17
Identity = 73/242 (30.17%), Postives = 126/242 (52.07%), Query Frame = 3
Query:   93 VGFLKLGVERLFLGFRDKDDTENILKTIKIHAPHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFN---AKIRVKLLNI---------KLSLGAQL--GGKRGFFGLNEXXXXXXXXXXXKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNV 776
            +GF + G+ R       +   ENI  +IK +   +V  E++ L+ ++ +LI  L + ET+S +L  ISN+L ++ +   +FE +R KYF  +     F    N   +KI+ K   I          L+ G+ L  GG+     + + LS  L +  LK +  NV R +K  +  EI++ ++ +      + EKL  +ERD  KLE  S +LFKL+E+N +FER+  +YF+++    + + N+
Sbjct:   59 LGF-EFGLSRFLSEINTEGLVENISNSIKRYVSISVCNEVRILLNKIVSLINRLNRLETNSKKLEKISNVLIKILKKTFEFESIRRKYFKHIYFINDFKKILNLNESKIKRKYSVIFRHGKKRFSHLNFGSGLIIGGENSLIEIEDGLSRYLTKQILKRLSQNVLRSIKNRLPHEIFKSLKAILKTVTDLIEKLRYLERDVVKLEHESELLFKLHEQNMEFERFHAQYFSEIDWTEIREKNL 299          

HSP 9 Score: 76.2554 bits (186), Expect = 5.676e-15
Identity = 62/206 (30.10%), Postives = 107/206 (51.94%), Query Frame = 3
Query:  153 TENILKTIKIHAPHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNI---KLSLGAQLGGKRGFFGLNEXXXXXXXXXXXKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNV 761
            ++N+L++IK   PH + K ++ ++  +  LI+ L   E D  +L   S LL++LH+ N++FE   A+YFS ++   +   +    I     N      S   Q   K GF  L   L  +        ++ N  + +K  VS  I  +++ LF E   +F+ L + E++  KLE I ++L+KL + N KFER R KYF ++++ +V
Sbjct:  218 SQNVLRSIKNRLPHEIFKSLKAILKTVTDLIEKLRYLERDVVKLEHESELLFKLHEQNMEFERFHAQYFSEIDWTEIREKNLKGDIEKPNGNEIEENKSYNFQFHEKHGFLELG--LFKVYTLSSFDRVIENYIKMMKSKVSQSIIIKVQNLFREIEYLFKGLNSREKNKRKLEYIIHLLYKLNDNNIKFERLRKKYFRELNMIDV 421          

HSP 10 Score: 59.3066 bits (142), Expect = 2.310e-9
Identity = 37/103 (35.92%), Postives = 57/103 (55.34%), Query Frame = 3
Query:  450 QLGGKRGFFGLNEXXXXXXXXXXXKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQN 758
            + GGK G  G    LS  L  +  + ++ N+   +K  VS+ +  E+RIL  + V +  +L  +E +S KLEKISN+L K+ +K  +FE  R KYF  +   N
Sbjct:   51 RFGGKNGILGFEFGLSRFLSEINTEGLVENISNSIKRYVSISVCNEVRILLNKIVSLINRLNRLETNSKKLEKISNVLIKILKKTFEFESIRRKYFKHIYFIN 153          

HSP 11 Score: 48.9062 bits (115), Expect = 4.449e-6
Identity = 38/46 (82.61%), Postives = 44/46 (95.65%), Query Frame = 3
Query:  834 IDTKHRQHSVKISGKVKTHGKIHHPXXXXXXXXXXXXXXXXHKKPT 971
            +D KH++ +VKISGKVKTHGK+HHPKVGGKIVIKSGKV+IKHKKPT
Sbjct: 2356 VDIKHKKPTVKISGKVKTHGKVHHPKVGGKIVIKSGKVEIKHKKPT 2401          

HSP 12 Score: 48.9062 bits (115), Expect = 4.449e-6
Identity = 38/46 (82.61%), Postives = 44/46 (95.65%), Query Frame = 3
Query:  834 IDTKHRQHSVKISGKVKTHGKIHHPXXXXXXXXXXXXXXXXHKKPT 971
            +D KH++ +VKISGKVKTHGK+HHPKVGGKIVIKSGKV+IKHKKPT
Sbjct: 2430 VDIKHKKPTVKISGKVKTHGKVHHPKVGGKIVIKSGKVEIKHKKPT 2475          
The following BLAST results are available for this feature:
BLAST of SMED30000022 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000022 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000022 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000022 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000022 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000022 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000022 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000022 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000022 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000022 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000022 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000022 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000022 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30000022 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000061316.19.248e-175100.00SMESG000061316.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30000022 ID=SMED30000022|Name=SMED30000022|organism=Schmidtea mediterranea sexual|type=transcript|length=971bp
TTCATCATCCTAAAATAAGTGGAAAAATAGTTGTAAGAGGTAGAAAATCA
AGAAATCCTCATCACCGATTCCATCTAGACACTCGGAAACAAGTAGGTTT
CTTAAAACTGGGAGTAGAACGATTATTCCTTGGATTCAGAGACAAAGATG
ATACTGAAAATATTCTAAAAACAATAAAAATACACGCCCCACATAACGTC
ATAAAGGAAATTCAACAACTGGTATGGGAAATGAGAACATTGATTCAAAC
CTTATACAAATATGAGACAGATTCAGATAGATTAATAACCATCAGCAATT
TGTTATATCGTCTACATCAAAATAATATCAAATTCGAAATGTTACGGGCA
AAATATTTCTCAAGACTCAACGTGAAAACATTGTTCGGCAGTAGTTTCAA
TGCAAAGATCCGAGTTAAACTACTCAATATTAAATTATCTCTCGGAGCAC
AACTTGGAGGTAAACGTGGATTCTTTGGATTAAACGAACGTCTAAGTAGT
TTGCTACCAAGATTAAGATTGAAAAATATCCTTTCGAATGTAGAAAGACA
TTTAAAACCAATTGTTTCCGTCGAAATATGGCAGGAAATAAGAATATTAT
TTATAGAAAGTGTAAAAATATTCGAAAAACTGACAACTATTGAAAGAGAC
TCCGGAAAATTAGAAAAGATCAGCAATATATTATTCAAACTGTATGAGAA
AAATGATAAATTTGAAAGATATCGAATCAAATATTTCACAAAAGTCTCAC
TTCAAAATGTCATTGATACCAATGTTAAAATTAAAATTAAACAGAAAAAG
ATTAATAAAAAATACCTAAAAATTGTTGGTAAAATTGATACAAAACATAG
ACAACATTCCGTTAAAATCTCTGGAAAAGTCAAAACTCACGGAAAAATTC
ATCATCCGAAAGTTGGTGGCAAAATTGTTATTAAAAGTGGAAAAGTTGAT
ATAAAACATAAGAAACCAACT
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protein sequence of SMED30000022-orf-1

>SMED30000022-orf-1 ID=SMED30000022-orf-1|Name=SMED30000022-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=323bp
HHPKISGKIVVRGRKSRNPHHRFHLDTRKQVGFLKLGVERLFLGFRDKDD
TENILKTIKIHAPHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNL
LYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNIKLSLGAQ
LGGKRGFFGLNERLSSLLPRLRLKNILSNVERHLKPIVSVEIWQEIRILF
IESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSL
QNVIDTNVKIKIKQKKINKKYLKIVGKIDTKHRQHSVKISGKVKTHGKIH
HPKVGGKIVIKSGKVDIKHKKPT
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0000419prepharyngeal region
PLANA:0000420parapharyngeal region
PLANA:0002142parenchyma